WO2006057377A1 - レムナント様リポタンパク質中のコレステロールの測定方法 - Google Patents
レムナント様リポタンパク質中のコレステロールの測定方法 Download PDFInfo
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- WO2006057377A1 WO2006057377A1 PCT/JP2005/021757 JP2005021757W WO2006057377A1 WO 2006057377 A1 WO2006057377 A1 WO 2006057377A1 JP 2005021757 W JP2005021757 W JP 2005021757W WO 2006057377 A1 WO2006057377 A1 WO 2006057377A1
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- cholesterol
- activity
- lipoprotein
- remnant
- cholesterol esterase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
Definitions
- the present invention relates to a method for measuring cholesterol in a remnant-like lipoprotein and a measurement reagent.
- arteriosclerosis occupies an important position in the development of cardiovascular diseases such as heart diseases and cerebrovascular diseases (cerebral infarction, etc.).
- Lipoprotein is composed of cholesterol, cholesterol ester, neutral fat, phospholipid, and apotanic protein.
- HDL high-density lipoprotein
- LDL low-density lipoprotein
- Patent Document 1 JP-A-2001-231597
- Patent Document 2 Japanese Patent No. 2949354
- Non-Patent Document 1 Arteriosclerosis, 25 (9, 10), 371 (1998)
- An object of the present invention is to provide a method for measuring cholesterol in a remnant-like protein more easily and accurately and a reagent for carrying out the method.
- cholesterol esterase has lipoprotein lipase activity in addition to esterase activity, and measures cholesterol in lipotan protein.
- cholesterol esterase having a higher ratio of lipoprotein lipase activity than cholesterol esterase activity was selected, and cholesterol oxidase or cholesterol dehydrogenase was used to select cholesterol esterase.
- HDL high density lipoprotein
- LDL low density lipoprotein
- CM chylomicron
- VLDL very low density lipoprotein
- IDL medium density lipoprotein
- the present invention allows cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase to act on a test sample containing lipoprotein, and measures the peroxyhydrogen or reduced coenzyme produced.
- the activity ratio of lipoprotein lipase and cholesterol esterase lipoprotein lipase activity Z cholesterol esterase
- the present invention relates to a method for measuring cholesterol in a remnant-like lipoprotein, wherein a cholesterol esterase having an activity of 12 to 7000 is used.
- the present invention relates to a cholesterol monoreesterase having a lipoprotein lipase and cholesterol esterase activity ratio (lipoprotein lipase activity Z cholesterol esterase activity) of 12 to 7000, and a cholesterol monoreoxidase or a cholesterol monodehydrogenase.
- the present invention relates to a reagent for measuring cholesterol in the contained remnant-like lipoprotein.
- FIG. 1 is a view showing the reaction specificity of LDL when LP enzyme is used.
- FIG. 2 shows the reaction specificity of HDL when LP enzyme is used.
- FIG. 3 is a diagram showing reaction specificity for unbound-VLDL and bound-VLDL in the method of the present invention.
- FIG. 4 is a diagram showing the reactivity to unbound-VLDL and bound-VLDL in antibody-added cells.
- FIG. 5 is a diagram showing a correlation when remnant-like lipoproteins in blood are measured in the measurement method of the present invention and the existing measurement method (RLP-IV).
- the method for measuring cholesterol in a remnant-like lipoprotein of the present invention comprises measuring cholesterol or reduced coenzyme produced by the action of cholesterol-free esterase and cholesterol-free reoxidase or cholesterol-free rehydrogenase.
- a cholesterol esterase having an activity ratio of lipoprotein lipase (LPL) to cholesterol esterase (CE) (lipoprotein lipase activity / cholesterol esterase activity) of 12 to 7000 is used.
- Cholesterol esterase is an enzyme that hydrolyzes cholesterol ester contained in lipoproteins to produce cholesterol.
- lipoprotein lipase activity that is, lipase activity that hydrolyzes neutral fat.
- the lipoprotein lipase activity is stronger than the cholesterol esterase activity.
- the activity ratio of lipoprotein lipase and cholesterol esterase is 12 to 7000. It is necessary to use a cholesterol esterase, which causes the enzyme to act selectively on the remnant-like lipoprotein and selectively hydrolyze the cholesterol ester in the protein, resulting in the desired remnant. Only cholesterol in lipoproteins can be measured selectively.
- the lipoprotein lipase activity indicates an activity unit calculated by a lipotan lipase activity assay using olive oil emulsion (Horiuchi Y. et a 1. J Biochem 1976; 80: 367). — 370.), cholesterol esterase activity refers to the activity unit calculated by CEN activity analysis using calf serum (Kameno Y. et al. Jap J Clin Path 1976; 24: 650.).
- the activity ratio of lipoprotein lipase and cholesterol esterase is a force of 12 to 7000, preferably 20 to: L000, more preferably 28-800.
- cholesterol esterase having such an activity ratio examples include LP (Asahi Kasei Pharma, activity ratio 460 to 800), LPBP (Asahi Kasei Pharma, activity ratio 560 0 to 6900), CEN (Asahi Kasei Pharma, active Ratio (13.0 to 16.0), CE (Amano Enzym, activity ratio 13.0 to 16.0), COE311 (Toyobo, activity ratio 28.0 to 35.0) or equivalent
- LP Aligni Kasei Pharma, activity ratio 460 to 800
- LPBP Asahi Kasei Pharma, activity ratio 560 0 to 6900
- CEN Asahi Kasei Pharma, active Ratio (13.0 to 16.0)
- CE Amano Enzym, activity ratio 13.0 to 16.0
- COE311 Toyobo, activity ratio 28.0 to 35.0 or equivalent
- the cholesterol esterase may be derived from microorganisms or animals as long as it satisfies the conditions.
- the amount of cholesterol esterase used depends on the content or physical properties of the remnant-like lipoprotein in the test sample. Usually 0.1 to 200 units ZmL is preferably used. : Can be used in the range of L00 units / mL. Usually, in healthy individuals, good measurement results can be obtained with 1-5 units ZmL. It should be noted that it is desirable to use 50 units ZmL or more among hyperlipidemia patients, especially in patients with in-family hyperlipidemia, for which a simple measurement method has been desired. Moreover, in healthy pregnant women, the ability to generally increase blood cholesterol levels can be obtained at 20 units / mL using the cholesterol esterase of the present invention.
- a test sample may be pretreated with anti-apo B-100 antibody or anti-apo A-I antibody.
- anti-apo B-100 antibody or anti-apo A-I antibody By adding such an antibody, it is possible to increase the selectivity of cholesterol esterase for LDL, VLDL having apo B-100 as a constituent, and HDL and CM having apo A-1 as a constituent.
- Such an antibody treatment technique is a force manipulation method disclosed in Japanese Patent No. 2949354, in which an antibody-binding gel is added to a test sample and left for several hours to allow remnant-like lipoproteins and others. It is aimed to isolate the lipoproteins (HDL, LDL, VLDL, CM) of the lipase and to increase the selectivity of cholesterol esterase secondarily.
- the cholesterol esterase of the present invention it is not necessary to immobilize the antibody with a gel, and the action of the enzyme on lipoproteins other than the remnant-like lipoprotein can be suppressed simply by adding the antibody. As a result, selectivity for remnant-like lipoprotein can be increased.
- the object can be achieved by the binding of the antibody and the lipoprotein. Therefore, it is possible to proceed to the next operation within a few minutes after the addition, and therefore, it is possible to dramatically improve the simplicity of the operation method, the measurement accuracy and the time reduction.
- any polyclonal antibody, monoclonal antibody or combination thereof can be used as long as it is an antibody against Apo B-100 or Apo A-I.
- B-100-recognizing antibodies include JI-H (manufactured by Japan Antibody Laboratories), and examples of apo A-I-recognizing antibodies include H-12 (manufactured by Japan Antibody Laboratories).
- apo A-I-recognizing antibodies include H-12 (manufactured by Japan Antibody Laboratories).
- the amount of antibody-added marker depends on the amount of HDL, LDL, VLDL or CM in the test sample, it is sufficient to add an amount capable of binding to the lipoprotein.
- Cholesterol oxidase oxidizes cholesterol to produce hydrogen peroxide
- the enzyme is not particularly limited as long as it has the ability to act, and examples thereof include cholesterol oxidase derived from microorganisms or animals.
- the cholesterol dehydrogenase is not particularly limited as long as it is an enzyme having the ability to oxidize cholesterol and reduce oxidized coenzyme, and examples thereof include microorganism or animal-derived cholesterol dehydrogenase.
- the amount of cholesterol oxidase and cholesterol dehydrogenase used is preferably 0.01 to 200 UZmL in the reaction solution, respectively 0.1 to LOOUZmL is more preferable.
- various metal ions are added to improve the specificity and stability of the enzyme. Can be used in a casual manner.
- Cholesterol monoreesterase, cholesterol monoreoxidase, and cholesterol monoredehydrogenase have a group mainly composed of polyethylene glycol, a water-soluble oligosaccharide residue, a sulfopropyl group and the like as long as they have equivalent activities.
- An improved enzyme having an equivalent activity obtained by genetic engineering may be used which may be modified chemically.
- the enzymatic reaction of the present invention is preferably carried out in a buffer solution, particularly in a force carried out in an aqueous medium.
- buffer examples include tris (hydroxymethyl) aminomethane, phosphate buffer, borate buffer, Good's buffer, and the like.
- Good buffering agents include N- (2-acetamido) 2-aminoethanesulfonic acid (ACES), N- (2-acetamido) iminoniacetic acid (AD A), N, N bis (2 hydroxyethyl) —2 Aminoethanesulfonic acid (BES), 3— [N, N Bis (2-hydroxyethyl) amino] — 2-Hydroxypropanesulfonic acid (DIPS 0), 2-— (2-Hydroxyethyl) piperazine N , 1 2-Ethanesulfonic acid (HEPES), 3- (N monophorino) propanesulfonic acid (MOPSO), piperazine-N, N, -bis (2-ethaneethanerephonic acid) (PIPES), piperazine 1 N, N, 1 bis (2-hydroxypropane-3-
- the pH of the buffer is 4-10, preferably 5-9.
- the concentration of the buffer used is preferably 5 to 5 OOmM, more preferably 10 to 200mM, and particularly preferably 20 to LOOmM.
- oxygen power and hydrogen peroxide are generated by the reaction of cholesterol.
- the generated hydrogen peroxide can be quantified, for example, in the presence of peroxidase using 4-aminoantipyrine and phenols, 4-aminoantipyrine and a Trinder reagent or a highly sensitive dye source.
- phenols examples include phenol, 4-chlorophenol, m-cresol, 3-hydroxy-2,4,6 tolyobenzoic acid (HTIB) and the like.
- the Trinder reagent (Ninjin Chemical Laboratory 19th edition general catalog, 2002) includes N-sulfopropylene, N-ethyl N- (2-hydroxy-1-sulfopropyl) m-toluidine (TOOS), N-Ethylu N— (2-Hydroxy-3-sulfopropyl) -3,5 Dimethylaline (MAOS), N-Ethylu N— (2 Hydroxy-3-sulfopropyl) -3,5-Dimethoxyaline (DAOS) ), N-ethyl-N-sulfopropyl-m-toluidine (TOPS), N- (2-hydroxy-m-toluidine, N, N-disrenopropynole-3,5-dimethoxyaniline, N-ethyl-N —Sulfopropyl—m-anisidine, N-ethyl-N-sulfopropylaniline, N-
- NAD (P) H which is a reduced coenzyme, is generated from NAD (P), which is an acid-containing coenzyme, by the reaction of cholesterol.
- NAD (P) H can be quantified by measuring the absorbance of the reaction solution at about 300 to 500 nm, preferably 330 to 400 nm, particularly preferably about 340 nm.
- NAD (P) H can also be quantified by adding a forazan dye by adding diaphorase or tetrazolium salt and then colorimetric formazan dye.
- the reaction may be carried out at 10 to 50 ° C, preferably 30 to 40 ° C, usually 37 ° C for 1 to 30 minutes, preferably 2 to 10 minutes.
- the reagent of the present invention has higher lipoprotein lipase activity than cholesterol esterase activity, and contains cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase.
- the reagent as the first reagent containing the antibody and the second reagent containing the enzyme solution.
- the first reagent is added to the test sample and reacted at 25-40 ° C for 1-10 minutes, then the second reagent is added and allowed to act at 25-40 ° C for 2-20 minutes. Is desirable.
- the measurement reagent can contain various salts, lipoprotein flocculants, other enzymes, and the like for solubilizing proteins such as globulins in a biological sample, if necessary.
- Such salts include sodium chloride, sodium sulfate, potassium salt, potassium sulfate, magnesium chloride, magnesium sulfate, magnesium acetate, lithium chloride, lithium sulfate, ammonium sulfate, magnesium nitrate, calcium nitrate.
- 0 ⁇ Used in LOO mM.
- lipoprotein flocculants include divalent metal salts such as phosphotungstate, dextran sulfate, heparin and other polyion-on magnesium, calcium and cobalt.
- Other enzymes include ascorbate oxidase. I can get lost.
- test sample applied to the present invention is not particularly limited. Specifically, for a biological sample, blood itself or blood fractions such as plasma and serum can be used. .
- Serum components were fractionated into an LDL fraction and an HDL fraction, and each lipoprotein was measured.
- the method for fractionating the protein is shown below.
- Anti-apo B-100 antibody-binding affinity columns and anti-apo A-I antibody-binding affinity columns were prepared, and serum was added to collect the bound fractions, which were designated as LDL and HDL, respectively.
- VLDL fractions were separated by ultracentrifugation (Havel RJ et al. J Clin Invest 195 5; 34: 1345., 120, OOOrpm, 1.5 hr, 4. (Total—VLDL) and then immunized.
- the fraction bound to the adsorption method was Boun d-VLDL, and the flow-through fraction was Unbound-VLDL (remnant-like lipoprotein fraction) Examples 2 and 3 In the measurement, cholesterol concentrations adjusted to 40 mgZd L were used.
- Reagent 1 When antibody pretreatment was performed, 210 L of Reagent 1 was placed in a spectrophotometer cell, and serum 2 O / z L was added and stirred, followed by heating at 37 ° C. Then 5 minutes later (reaction point
- reaction point 33 70 ⁇ L of Reagent 2 previously heated to 37 ° C was added, and then measured until 5 minutes (reaction point 33).
- the measurement wavelength is 7 at the main wavelength of 572nm and the sub wavelength of 748nm.
- the standard for evaluation was the rate of change in absorbance up to reaction point 17-33.
- Cholesterol was measured using LDL and HDL treated with 0.1% surfactant (Emulgen, Kao) and an untreated solution as a test sample.
- LP enzyme Asahi Kasei Pharma, activity ratio 512
- CE enzyme Automatic Enzym, activity ratio 14.3
- Figure 1 shows the LDL
- Figure 2 shows the HDL.
- the measurement method was in accordance with Example 1. In both cases, the reactivity was significantly reduced when LP enzyme was used compared to the total cholesterol reaction (treated with surfactant). When CE enzyme was used, the absorption was higher than that of LP enzyme.
- the reaction specificity of the VLDL fraction in the measurement method of the present invention was examined using LP enzyme.
- the measurement method was in accordance with Example 1.
- Fig. 3 shows the reactivity of the anti-apo B-100 antibody (JI-H) and anti-apo A-I antibody (H-12) not added
- Fig. 4 shows the reactivity in the antibody-added column.
- the antibody is added to the first solution so that the final concentration of both JI-H and H-12 is 100 gZmL. It was.
- FIG. 3 and FIG. 4 showed similar reaction curves for both unbound-VLDL and bound-VLDL, confirming the selective measurement of cholesterol in remnant-like lipoproteins.
- the bound-VLDL reactivity was further reduced to about 1 Z3, and the enzyme reaction was suppressed.
- the remnant-like lipoprotein can be measured with higher accuracy by the antibody-added column.
- Lipoprotein lipase (L P L) activity L P activity analysis method (Oriip oil oil marjool cholesterol esterase (C E) activity: CE activity analysis method (calf serum).
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Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
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JP2006547888A JP4842838B2 (ja) | 2004-11-29 | 2005-11-28 | レムナント様リポタンパク質中のコレステロールの測定方法 |
CN2005800407163A CN101065495B (zh) | 2004-11-29 | 2005-11-28 | 残粒样脂蛋白中胆固醇的测定方法 |
US11/720,338 US7799537B2 (en) | 2004-11-29 | 2005-11-28 | Cholesterol measuring reagent containing a cholesterol esterase |
DE602005020678T DE602005020678D1 (de) | 2004-11-29 | 2005-11-28 | Verfahren zur cholesterinmessung in übrigbleibenden lipoproteinen |
EP05809660A EP1818412B1 (en) | 2004-11-29 | 2005-11-28 | Method of measuring cholesterol in remnant-like lipoproteins |
CA2589459A CA2589459C (en) | 2004-11-29 | 2005-11-28 | Method of measuring cholesterol in remnant-like lipoproteins |
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US (1) | US7799537B2 (ja) |
EP (1) | EP1818412B1 (ja) |
JP (1) | JP4842838B2 (ja) |
CN (1) | CN101065495B (ja) |
CA (1) | CA2589459C (ja) |
DE (1) | DE602005020678D1 (ja) |
ES (1) | ES2344304T3 (ja) |
WO (1) | WO2006057377A1 (ja) |
Cited By (1)
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WO2012063866A1 (ja) | 2010-11-10 | 2012-05-18 | デンカ生研株式会社 | レムナント様リポ蛋白コレステロールの定量方法及びそのためのキット |
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WO2012118017A1 (ja) * | 2011-02-28 | 2012-09-07 | デンカ生研株式会社 | 高密度リポタンパク質2中のコレステロールの定量方法およびそのための試薬キット |
US10077286B2 (en) * | 2012-04-20 | 2018-09-18 | Denka Seiken Co., Ltd. | Method for removal of triglycerides in lipoproteins other than low-density lipoproteins |
CN105044364B (zh) * | 2015-05-02 | 2016-09-14 | 王贤俊 | 一种采用联合遮蔽法制备的ldl-c检测试剂盒 |
CN106645763B (zh) * | 2017-01-03 | 2019-03-22 | 长沙中生众捷生物技术有限公司 | 总胆固醇的检测试纸 |
CN106950095B (zh) * | 2017-04-01 | 2020-01-10 | 合肥迪安医学检验所有限公司 | 一种减少脂血样本检测干扰的方法 |
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JP2001231597A (ja) * | 2000-02-28 | 2001-08-28 | Kyowa Medex Co Ltd | レムナント様リポ蛋白中のコレステロールの測定方法および測定試薬 |
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JPH03251304A (ja) | 1990-02-27 | 1991-11-08 | Sumitomo Electric Ind Ltd | Si↓3N↓4焼結体製マイクロドリル |
ATE446378T1 (de) * | 2000-06-07 | 2009-11-15 | Sysmex Corp | Verfahren zur analyse von komponenten biologischer proben |
KR20060127111A (ko) * | 2004-01-29 | 2006-12-11 | 교와 메덱스 가부시키가이샤 | 초저밀도 지단백질 잔유물 (vldl 잔유물) 중의콜레스테롤 정량 방법, 시약 및 키트 |
WO2006085654A1 (ja) * | 2005-02-14 | 2006-08-17 | Kyowa Medex Co., Ltd. | レムナント様リポ蛋白(rlp)中のコレステロールの定量方法、試薬およびキット |
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JPH03245058A (ja) * | 1990-02-22 | 1991-10-31 | Nippon Koutai Kenkyusho:Kk | 変性リポタンパクの測定法及びこれに用いるキット |
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WANG T ET AL: "Ratio of Remnant-like Particle-Cholesterol to Serum Total Triglycerides is an Effective Alternative to Ultracentrifugal and Electrophoretic Methods in the Diagnosis of Familial Type III Hyperlipoproteinemia.", CLINICAL CHEMISTRY., vol. 45, 1999, pages 1981 - 1987, XP003007142 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012063866A1 (ja) | 2010-11-10 | 2012-05-18 | デンカ生研株式会社 | レムナント様リポ蛋白コレステロールの定量方法及びそのためのキット |
JP2012100581A (ja) * | 2010-11-10 | 2012-05-31 | Denka Seiken Co Ltd | レムナント様リポ蛋白コレステロールの定量方法及びそのためのキット |
KR20140027060A (ko) | 2010-11-10 | 2014-03-06 | 덴카 세이켄 가부시키가이샤 | 렘난트형 리포단백질 콜레스테롤의 정량 방법 및 그것을 위한 키트 |
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EP1818412A4 (en) | 2008-06-04 |
CN101065495B (zh) | 2011-05-04 |
DE602005020678D1 (de) | 2010-05-27 |
US20080268461A1 (en) | 2008-10-30 |
EP1818412A1 (en) | 2007-08-15 |
JPWO2006057377A1 (ja) | 2008-06-05 |
CA2589459A1 (en) | 2006-06-01 |
ES2344304T3 (es) | 2010-08-24 |
CN101065495A (zh) | 2007-10-31 |
JP4842838B2 (ja) | 2011-12-21 |
CA2589459C (en) | 2014-01-07 |
EP1818412B1 (en) | 2010-04-14 |
US7799537B2 (en) | 2010-09-21 |
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