WO2006006960A1 - One-step enzymatic and amine detection technique - Google Patents

One-step enzymatic and amine detection technique Download PDF

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Publication number
WO2006006960A1
WO2006006960A1 PCT/US2005/011050 US2005011050W WO2006006960A1 WO 2006006960 A1 WO2006006960 A1 WO 2006006960A1 US 2005011050 W US2005011050 W US 2005011050W WO 2006006960 A1 WO2006006960 A1 WO 2006006960A1
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WO
WIPO (PCT)
Prior art keywords
enzyme
amine
detection zone
test kit
diagnostic test
Prior art date
Application number
PCT/US2005/011050
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English (en)
French (fr)
Inventor
Xuedong Song
Rameshbabu Boga
Chibueze Obi Chidebelu-Eze
Original Assignee
Kimberly-Clark Worldwide, Inc.
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Publication date
Application filed by Kimberly-Clark Worldwide, Inc. filed Critical Kimberly-Clark Worldwide, Inc.
Publication of WO2006006960A1 publication Critical patent/WO2006006960A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PCT/US2005/011050 2004-06-30 2005-03-31 One-step enzymatic and amine detection technique WO2006006960A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/881,010 2004-06-30
US10/881,010 US7521226B2 (en) 2004-06-30 2004-06-30 One-step enzymatic and amine detection technique

Publications (1)

Publication Number Publication Date
WO2006006960A1 true WO2006006960A1 (en) 2006-01-19

Family

ID=34964751

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/011050 WO2006006960A1 (en) 2004-06-30 2005-03-31 One-step enzymatic and amine detection technique

Country Status (2)

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US (2) US7521226B2 (US07521226-20090421-C00009.png)
WO (1) WO2006006960A1 (US07521226-20090421-C00009.png)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1920253A2 (en) * 2005-08-31 2008-05-14 Kimberly-Clark Worldwide, Inc. Detection of proteases secreted from pathogenic microorganisms

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7413550B2 (en) 2003-10-16 2008-08-19 Kimberly-Clark Worldwide, Inc. Visual indicating device for bad breath
US7582485B2 (en) * 2003-10-16 2009-09-01 Kimberly-Clark Worldride, Inc. Method and device for detecting ammonia odors and helicobacter pylori urease infection
US7906276B2 (en) 2004-06-30 2011-03-15 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
US8003399B2 (en) 2005-08-31 2011-08-23 Kimberly-Clark Worldwide, Inc. Nitrite detection technique
US7504235B2 (en) 2005-08-31 2009-03-17 Kimberly-Clark Worldwide, Inc. Enzyme detection technique
US8758989B2 (en) 2006-04-06 2014-06-24 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
US8044257B2 (en) * 2006-10-30 2011-10-25 Kimberly-Clark Worldwide, Inc. Absorbent article containing lateral flow assay device
US7897360B2 (en) 2006-12-15 2011-03-01 Kimberly-Clark Worldwide, Inc. Enzyme detection techniques
US9034593B2 (en) 2010-11-22 2015-05-19 Kimberly-Clark Worldwide, Inc. Vaginal indicator to detect biomarkers of good health
NZ703227A (en) * 2012-06-22 2016-10-28 Scandinavian Micro Biodevices Aps A method and a system for quantitative or qualitative determination of a target component
CN105044093A (zh) * 2015-06-26 2015-11-11 上海纳米技术及应用国家工程研究中心有限公司 纳米氧化锰、氧化铬作为过氧化物模拟酶检测过氧化氢的应用
CN106872420B (zh) * 2016-12-27 2019-12-17 厦门奥德生物科技有限公司 一种时间分辨荧光定量检测尿微量白蛋白的试剂盒及方法
CN107192823B (zh) * 2017-06-08 2024-03-19 杭州遂真生物技术有限公司 一种b族链球菌酶联免疫检测试剂盒
CN112798779A (zh) * 2019-11-13 2021-05-14 中粮集团有限公司 一种量子点标记的直接竞争荧光免疫检测超氧化物歧化酶的方法
CN111505291B (zh) * 2020-04-14 2023-04-25 山东省千佛山医院 一种排除巨酶分子对血清酶浓度检测带来干扰的方法
CN113358639B (zh) * 2021-06-15 2022-06-21 山东大学 一种基于酶联显色法检测乙酸根含量的方法及其应用

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0244932A2 (en) * 1986-03-10 1987-11-11 Board Of Regents, The University Of Texas System Solid-phase substrate yielding soluble labeled products upon hydrolysis by a glycosaminoglycan endoglycosidase
US5124254A (en) * 1988-02-08 1992-06-23 University College Cardiff Consultants Limited Detection of diamines in biological fluids
US5292652A (en) * 1990-10-05 1994-03-08 Athena Neurosciences, Inc. Amyloidin protease and uses thereof
US5585273A (en) * 1993-04-14 1996-12-17 Litmus Concepts, Inc. Test device for assays for hydrolytic enzyme activity
US6243980B1 (en) * 1997-03-07 2001-06-12 Tropix, Inc. Protease inhibitor assay
US6451619B1 (en) * 1994-06-29 2002-09-17 Inverness Medical Switzerland Gmbh Monitoring methods and devices for use therein
WO2003085403A1 (en) * 2002-03-30 2003-10-16 Xenotope Diagnostics, Inc. Method and device for trichomonas detection
US20040121480A1 (en) * 2002-12-19 2004-06-24 Kimberly-Clark Worldwide, Inc. Reduction of the hook effect in membrane-based assay devices

Family Cites Families (274)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1366241A (en) * 1919-10-03 1921-01-18 Frederick W Burch Ratchet mechanism for camp-beds
US3772076A (en) 1970-01-26 1973-11-13 Hercules Inc Reaction products of epihalohydrin and polymers of diallylamine and their use in paper
US3700623A (en) 1970-04-22 1972-10-24 Hercules Inc Reaction products of epihalohydrin and polymers of diallylamine and their use in paper
CS179075B1 (en) 1974-11-26 1977-10-31 Stoy Vladimir Mode of manufacture of spherical particles from polymer
SE388694B (sv) 1975-01-27 1976-10-11 Kabi Ab Sett att pavisa ett antigen exv i prov av kroppvetskor, med utnyttjande av till porost berarmaterial bundna eller adsorberande antikroppar
USRE30267E (en) * 1975-06-20 1980-05-06 Eastman Kodak Company Multilayer analytical element
US4094647A (en) 1976-07-02 1978-06-13 Thyroid Diagnostics, Inc. Test device
US4210723A (en) 1976-07-23 1980-07-01 The Dow Chemical Company Method of coupling a protein to an epoxylated latex
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4374925A (en) * 1978-11-24 1983-02-22 Syva Company Macromolecular environment control in specific receptor assays
US4441373A (en) * 1979-02-21 1984-04-10 American Hospital Supply Corporation Collection tube for drawing samples of biological fluids
US4312228A (en) * 1979-07-30 1982-01-26 Henry Wohltjen Methods of detection with surface acoustic wave and apparati therefor
US5432057A (en) 1979-12-26 1995-07-11 Syva Company Simultaneous calibration heterogeneous immunoassay
US4849338A (en) 1982-07-16 1989-07-18 Syntex (U.S.A.) Inc. Simultaneous calibration heterogeneous immunoassay
US4843000A (en) 1979-12-26 1989-06-27 Syntex (U.S.A.) Inc. Simultaneous calibration heterogeneous immunoassay
US4299916A (en) 1979-12-26 1981-11-10 Syva Company Preferential signal production on a surface in immunoassays
US4533629A (en) 1981-04-17 1985-08-06 Syva Company Simultaneous calibration heterogeneous immunoassay
US5156953A (en) 1979-12-26 1992-10-20 Syntex (U.S.A.) Inc. Simultaneous calibration heterogeneous immunoassay
US4540659A (en) 1981-04-17 1985-09-10 Syva Company Simultaneous calibration heterogeneous immunoassay
CH648052A5 (de) 1980-02-14 1985-02-28 Ciba Geigy Ag Verfahren zur herstellung von triarylmethanverbindungen.
US4427836A (en) * 1980-06-12 1984-01-24 Rohm And Haas Company Sequential heteropolymer dispersion and a particulate material obtainable therefrom, useful in coating compositions as a thickening and/or opacifying agent
US4366241A (en) 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
WO1982000641A1 (en) * 1980-08-25 1982-03-04 Ab Kabivitrum Peptide substrates for determination of protease activity
US4385126A (en) * 1980-11-19 1983-05-24 International Diagnostic Technology, Inc. Double tagged immunoassay
US4426451A (en) * 1981-01-28 1984-01-17 Eastman Kodak Company Multi-zoned reaction vessel having pressure-actuatable control means between zones
US4442204A (en) * 1981-04-10 1984-04-10 Miles Laboratories, Inc. Homogeneous specific binding assay device and preformed complex method
US4444592A (en) * 1981-06-02 1984-04-24 The Sherwin-Williams Company Pigment compositions and processes therefor
US4363874A (en) 1981-08-07 1982-12-14 Miles Laboratories, Inc. Multilayer analytical element having an impermeable radiation nondiffusing reflecting layer
EP0073593A1 (en) 1981-09-01 1983-03-09 E.I. Du Pont De Nemours And Company Size-exclusion heterogeneous immunoassay
US4480042A (en) 1981-10-28 1984-10-30 E. I. Du Pont De Nemours And Company Covalently bonded high refractive index particle reagents and their use in light scattering immunoassays
US4477635A (en) 1982-01-04 1984-10-16 Minnesota Mining And Manufacturing Company Polymeric triarylmethane dyes
US4435504A (en) * 1982-07-15 1984-03-06 Syva Company Immunochromatographic assay with support having bound "MIP" and second enzyme
US4534356A (en) 1982-07-30 1985-08-13 Diamond Shamrock Chemicals Company Solid state transcutaneous blood gas sensors
US4632559A (en) 1982-11-29 1986-12-30 Miles Laboratories, Inc. Optical readhead
US4537861A (en) 1983-02-03 1985-08-27 Elings Virgil B Apparatus and method for homogeneous immunoassay
GB8314523D0 (en) 1983-05-25 1983-06-29 Lowe C R Diagnostic device
EP0127797B1 (de) 1983-06-03 1987-06-16 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Markermoleküle für Fluoreszenz-Immuno-Assays sowie Verfahren und Zwischenprodukte zu deren Herstellung
CH662421A5 (de) 1983-07-13 1987-09-30 Suisse Horlogerie Rech Lab Piezoelektrischer kontaminationsdetektor.
US4537657A (en) 1983-08-26 1985-08-27 Hercules Incorporated Wet strength resins
US4552458A (en) 1983-10-11 1985-11-12 Eastman Kodak Company Compact reflectometer
US4595661A (en) 1983-11-18 1986-06-17 Beckman Instruments, Inc. Immunoassays and kits for use therein which include low affinity antibodies for reducing the hook effect
US4703017C1 (en) 1984-02-14 2001-12-04 Becton Dickinson Co Solid phase assay with visual readout
US4698262A (en) 1984-04-27 1987-10-06 Becton, Dickinson And Company Fluorescently labeled microbeads
US4632901A (en) * 1984-05-11 1986-12-30 Hybritech Incorporated Method and apparatus for immunoassays
US4586695A (en) * 1984-06-22 1986-05-06 Miller Charlie D Continuous tube extractor
FI842992A0 (fi) * 1984-07-26 1984-07-26 Labsystems Oy Immunologiskt definitionsfoerfarande.
US4661235A (en) * 1984-08-03 1987-04-28 Krull Ulrich J Chemo-receptive lipid based membrane transducers
US4596697A (en) 1984-09-04 1986-06-24 The United States Of America As Represented By The Secretary Of The Army Chemical sensor matrix
US5310687A (en) * 1984-10-31 1994-05-10 Igen, Inc. Luminescent metal chelate labels and means for detection
US4722889A (en) * 1985-04-02 1988-02-02 Leeco Diagnostics, Inc. Immunoassays using multiple monoclonal antibodies and scavenger antibodies
US5026653A (en) 1985-04-02 1991-06-25 Leeco Diagnostic, Inc. Scavenger antibody mixture and its use for immunometric assay
CA1272127A (en) 1985-04-04 1990-07-31 Hybritech Incorporated Solid phase system for use in ligand-receptor assays
US4743542A (en) * 1985-04-11 1988-05-10 Ortho Diagnostic Method for forestalling the hook effect in a multi-ligand immunoassay system
GB8509492D0 (en) 1985-04-12 1985-05-15 Plessey Co Plc Optical assay
US4963498A (en) 1985-08-05 1990-10-16 Biotrack Capillary flow device
US5238815A (en) 1985-08-30 1993-08-24 Toyo Soda Manufacturing Co., Ltd. Enzymatic immunoassay involving detecting fluorescence while oscillating magnetic beads
US5500350A (en) * 1985-10-30 1996-03-19 Celltech Limited Binding assay device
US4917503A (en) * 1985-12-02 1990-04-17 Lifelines Technology, Inc. Photoactivatable leuco base time-temperature indicator
CA1291031C (en) 1985-12-23 1991-10-22 Nikolaas C.J. De Jaeger Method for the detection of specific binding agents and their correspondingbindable substances
US5585279A (en) 1986-01-23 1996-12-17 Davidson; Robert S. Time-resolved luminescence binding assays using a fluorescent transition metal label other than ruthenium
US4916056A (en) * 1986-02-18 1990-04-10 Abbott Laboratories Solid-phase analytical device and method for using same
US5482830A (en) * 1986-02-25 1996-01-09 Biostar, Inc. Devices and methods for detection of an analyte based upon light interference
US4776944A (en) 1986-03-20 1988-10-11 Jiri Janata Chemical selective sensors utilizing admittance modulated membranes
US5591581A (en) * 1986-04-30 1997-01-07 Igen, Inc. Electrochemiluminescent rhenium moieties and methods for their use
EP0272320B1 (en) 1986-06-17 1994-03-23 Baxter Diagnostics Inc. Homogeneous fluoroassay methods employing fluorescent background rejection
GB8618133D0 (en) * 1986-07-24 1986-09-03 Pa Consulting Services Biosensors
JPH0692969B2 (ja) 1986-07-30 1994-11-16 株式会社シノテスト 免疫的測定方法
US5182135A (en) * 1986-08-12 1993-01-26 Bayer Aktiengesellschaft Process for improving the adherency of metallic coatings deposited without current on plastic surfaces
GB2197065A (en) 1986-11-03 1988-05-11 Stc Plc Optical sensor device
US4857453A (en) 1987-04-07 1989-08-15 Syntex (U.S.A.) Inc. Immunoassay device
US4855240A (en) 1987-05-13 1989-08-08 Becton Dickinson And Company Solid phase assay employing capillary flow
US4842783A (en) 1987-09-03 1989-06-27 Cordis Corporation Method of producing fiber optic chemical sensors incorporating photocrosslinked polymer gels
US5670381A (en) 1988-01-29 1997-09-23 Abbott Laboratories Devices for performing ion-capture binding assays
US5268306A (en) 1988-02-29 1993-12-07 Boehringer Mannheim Gmbh Preparation of a solid phase matrix containing a bound specific binding pair
US5145784A (en) 1988-05-04 1992-09-08 Cambridge Biotech Corporation Double capture assay method employing a capillary flow device
EP0341928A1 (en) 1988-05-10 1989-11-15 AMERSHAM INTERNATIONAL plc Improvements relating to surface plasmon resonance sensors
DE68907519T2 (de) 1988-05-10 1993-10-21 Amersham Int Plc Biosensoren.
GB8811919D0 (en) 1988-05-20 1988-06-22 Amersham Int Plc Biological sensors
GB8813307D0 (en) 1988-06-06 1988-07-13 Amersham Int Plc Biological sensors
US4877586A (en) 1988-07-27 1989-10-31 Eastman Kodak Company Sliding test device for assays
US5075077A (en) 1988-08-02 1991-12-24 Abbott Laboratories Test card for performing assays
AT390517B (de) * 1988-08-04 1990-05-25 Avl Verbrennungskraft Messtech Optischer sensor und verfahren zu dessen herstellung
US4962024A (en) 1988-08-11 1990-10-09 Becton, Dickinson And Company Signal enhancement in assay for an enzyme
US4973670A (en) 1988-08-12 1990-11-27 The Dow Chemical Company Method for preparing hollow latexes
US5252459A (en) 1988-09-23 1993-10-12 Abbott Laboratories Indicator reagents, diagnostic assays and test kits employing organic polymer latex particles
EP0363504A1 (en) 1988-10-10 1990-04-18 Dräger Nederland B.V. Method of providing a substrate with a layer comprising a polyvinylbased hydrogel and a biochemically active material
US6448091B1 (en) 1988-11-03 2002-09-10 Igen International, Inc. Method and apparatus for improved luminescence assays using particle concentration chemiluminescence detection
SE8902043L (sv) 1988-11-10 1990-05-11 Pharmacia Ab Foerfarande foer karakterisering av makromolekyler
SE462454B (sv) 1988-11-10 1990-06-25 Pharmacia Ab Maetyta foer anvaendning i biosensorer
SE8804074D0 (sv) * 1988-11-10 1988-11-10 Pharmacia Ab Sensorenhet och dess anvaendning i biosensorsystem
US5003178A (en) * 1988-11-14 1991-03-26 Electron Vision Corporation Large-area uniform electron source
US5063081A (en) 1988-11-14 1991-11-05 I-Stat Corporation Method of manufacturing a plurality of uniform microfabricated sensing devices having an immobilized ligand receptor
MY104234A (en) * 1988-11-17 1994-02-28 Becton Dickinson Co Immunoassay on a preblocked solid surface
DE68920126T2 (de) * 1988-11-23 1995-05-11 Cytec Tech Corp Poröse Polymerperlen und Verfahren.
US4940734A (en) 1988-11-23 1990-07-10 American Cyanamid Process for the preparation of porous polymer beads
US4895017A (en) * 1989-01-23 1990-01-23 The Boeing Company Apparatus and method for early detection and identification of dilute chemical vapors
US5096671A (en) * 1989-03-15 1992-03-17 Cordis Corporation Fiber optic chemical sensors incorporating electrostatic coupling
US5120662A (en) 1989-05-09 1992-06-09 Abbott Laboratories Multilayer solid phase immunoassay support and method of use
US5234813A (en) 1989-05-17 1993-08-10 Actimed Laboratories, Inc. Method and device for metering of fluid samples and detection of analytes therein
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) * 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
JPH0366384A (ja) 1989-08-04 1991-03-22 Senjiyu Seiyaku Kk 生理活性物質放出制御システム
US5235238A (en) 1989-08-10 1993-08-10 Dainabot Company, Limited Electrode-separated piezoelectric crystal oscillator and method for measurement using the electrode-separated piezoelectric crystal oscillator
AU635314B2 (en) * 1989-09-08 1993-03-18 Terumo Kabushiki Kaisha Measuring apparatus
CA2003942A1 (en) 1989-09-26 1991-03-26 Julie Lia Rudolph Solid assay support systems
GB8923699D0 (en) 1989-10-20 1989-12-06 Univ Strathclyde Apparatus for assessing a particular property in a medium
US5225935A (en) 1989-10-30 1993-07-06 Sharp Kabushiki Kaisha Optical device having a microlens and a process for making microlenses
US5252743A (en) 1989-11-13 1993-10-12 Affymax Technologies N.V. Spatially-addressable immobilization of anti-ligands on surfaces
GB8927503D0 (en) 1989-12-04 1990-02-07 Kronem Systems Inc Enzyme-amplified lanthanide chelate luminescence
US5508171A (en) * 1989-12-15 1996-04-16 Boehringer Mannheim Corporation Assay method with enzyme electrode system
ES2092523T3 (es) 1990-05-09 1996-12-01 Abbott Lab Ensayos que utilizan enlaces con recuperacion de conjugado.
US5139934A (en) 1990-05-25 1992-08-18 Becton, Dickinson And Company Substrate composition and method for solid phase urease immunoassay
DK138090D0 (da) * 1990-06-06 1990-06-06 Novo Nordisk As Diagnostisk analysemetode
US5200084A (en) * 1990-09-26 1993-04-06 Immunicon Corporation Apparatus and methods for magnetic separation
US5076094A (en) 1990-10-03 1991-12-31 The United States Of America As Represented By The United States Department Of Energy Dual output acoustic wave sensor for molecular identification
US5700636A (en) 1990-10-19 1997-12-23 Becton Dickinson And Company Methods for selectively detecting microorganisms associated with vaginal infections in complex biological samples
US5726064A (en) * 1990-11-22 1998-03-10 Applied Research Systems Ars Holding Nv Method of assay having calibration within the assay
US6027944A (en) * 1990-11-22 2000-02-22 Applied Research Systems Ars Holding Nv Capillary-fill biosensor device comprising a calibration zone
US5510481A (en) * 1990-11-26 1996-04-23 The Regents, University Of California Self-assembled molecular films incorporating a ligand
US5208535A (en) * 1990-12-28 1993-05-04 Research Development Corporation Of Japan Mr position detecting device
US5834226A (en) 1991-01-31 1998-11-10 Xytronyx, Inc. One-step test for aspartate aminotransferase
GB9102646D0 (en) * 1991-02-07 1991-03-27 Fisons Plc Analytical device
IL97318A0 (en) 1991-02-20 1992-05-25 Diagnostic Markers Inc Method for the very rapid detection of polyamines
US5196350A (en) * 1991-05-29 1993-03-23 Omnigene, Inc. Ligand assay using interference modulation
JPH06508214A (ja) 1991-05-30 1994-09-14 アボツト・ラボラトリーズ イオン捕獲ジゴキシンアッセイを実施するための方法及び試薬
JPH06508211A (ja) 1991-05-30 1994-09-14 アボツト・ラボラトリーズ ２ステップイオン捕獲結合アッセイを実施するための試薬及び方法
WO1992021769A1 (en) 1991-05-30 1992-12-10 Abbott Laboratories Reagents containing a nonspecific binding blocker in ion-capture binding assays
US5179288A (en) * 1991-09-30 1993-01-12 Ortho Pharmaceutical Corporation Apparatus and method for measuring a bodily constituent
US5418136A (en) * 1991-10-01 1995-05-23 Biostar, Inc. Devices for detection of an analyte based upon light interference
EP0608370B1 (en) * 1991-10-15 1998-01-07 Multilyte Limited Binding assay employing labelled reagent
US5424219A (en) 1991-10-25 1995-06-13 Cytech Biomedical, Inc. Method of performing assays for biomolecules and solid supports for use in such methods
EP0643777A4 (en) 1992-01-22 1995-06-07 Abbott Lab CALIBRATION REAGENTS FOR SEMI-QUANTITATIVE BINDING ASSAYS AND DEVICES.
US5137609A (en) 1992-01-31 1992-08-11 Biometric Imaging Inc. Differential separation assay
US5221454A (en) 1992-01-31 1993-06-22 Biometric Imaging Inc. Differential separation assay
US5885527A (en) * 1992-05-21 1999-03-23 Biosite Diagnostics, Inc. Diagnostic devices and apparatus for the controlled movement of reagents without membrances
EP0606422B1 (en) * 1992-07-02 1997-09-03 SOINI, Erkki Biospecific multiparameter assay method
US5395754A (en) * 1992-07-31 1995-03-07 Hybritech Incorporated Membrane-based immunoassay method
GB9217864D0 (en) * 1992-08-21 1992-10-07 Unilever Plc Monitoring method
US5356782A (en) 1992-09-03 1994-10-18 Boehringer Mannheim Corporation Analytical test apparatus with on board negative and positive control
DE59304876D1 (de) 1992-09-14 1997-02-06 Siemens Ag Gassensor
GB9221329D0 (en) * 1992-10-10 1992-11-25 Delta Biotechnology Ltd Preparation of further diagnostic agents
GB2273772A (en) 1992-12-16 1994-06-29 Granta Lab Ltd Detection of macromolecules utilising light diffraction
US5358852A (en) 1992-12-21 1994-10-25 Eastman Kodak Company Use of calcium in immunoassay for measurement of C-reactive protein
TW239881B (US07521226-20090421-C00009.png) 1992-12-22 1995-02-01 Sienna Biotech Inc
US6200820B1 (en) * 1992-12-22 2001-03-13 Sienna Biotech, Inc. Light scatter-based immunoassay
US5422726A (en) * 1993-02-16 1995-06-06 Tyler; Jonathan M. Solid state spectrofluorimeter and method of using the same
DE4309393A1 (de) 1993-03-23 1994-09-29 Boehringer Mannheim Gmbh Verringerung des Hook-Effekts in Immuntests mit teilchenförmigem Trägermaterial
DE4310142A1 (de) * 1993-03-29 1994-10-06 Boehringer Mannheim Gmbh Immunologisch aktive Konjugate und ein Verfahren zu ihrer Herstellung
JP3479100B2 (ja) * 1993-06-02 2003-12-15 帝国臓器製薬株式会社 免疫化学的簡易半定量方法および装置
US5658443A (en) 1993-07-23 1997-08-19 Matsushita Electric Industrial Co., Ltd. Biosensor and method for producing the same
US5512131A (en) * 1993-10-04 1996-04-30 President And Fellows Of Harvard College Formation of microstamped patterns on surfaces and derivative articles
KR0177182B1 (ko) * 1993-10-20 1999-05-15 최근선 중공구조를 갖는 유화중합체의 제조방법
DK0653625T3 (da) * 1993-11-12 2003-01-13 Inverness Medical Switzerland Aflæseindretninger til teststrimler
PT653639E (pt) * 1993-11-12 2000-06-30 Unilever Nv Equipamentos analiticos e metodos para a sua utilizacao
US5527711A (en) 1993-12-13 1996-06-18 Hewlett Packard Company Method and reagents for binding chemical analytes to a substrate surface, and related analytical devices and diagnostic techniques
JP3504750B2 (ja) 1993-12-22 2004-03-08 オルソ−クリニカル ダイアグノスティクス，インコーポレイティド 検量関係式の再校正法及び定量試験キット
GB9416002D0 (en) * 1994-08-08 1994-09-28 Univ Cranfield Fluid transport device
US5464739A (en) 1994-08-22 1995-11-07 Bayer Corporation Composition method for determining the presence of leukocyte cells, esterase or protease in a test sample
AUPM769394A0 (en) 1994-08-25 1994-09-15 Commonwealth Scientific And Industrial Research Organisation Assay for the detection of proteases
US6117090A (en) 1994-08-25 2000-09-12 Caillouette; James C. Method and apparatus for detecting amine producing organisms in the vagina
US5599668A (en) * 1994-09-22 1997-02-04 Abbott Laboratories Light scattering optical waveguide method for detecting specific binding events
DE69524337T2 (de) 1994-09-23 2002-10-17 Unilever Nv Überwachungsverfahren und dazu verwendbare Vorrichtungen
US5620850A (en) * 1994-09-26 1997-04-15 President And Fellows Of Harvard College Molecular recognition at surfaces derivatized with self-assembled monolayers
US20010046668A1 (en) 1994-10-21 2001-11-29 G.D. Searle & Co. Fluorescence polarization method for determining protease activity
US5571684A (en) 1994-11-07 1996-11-05 Litmus Concepts, Inc. Assay for proline iminopeptidase and other hydrolytic activities
AUPM950094A0 (en) 1994-11-16 1994-12-08 Australian Membrane And Biotechnology Research Institute Detection device and method
US5866434A (en) * 1994-12-08 1999-02-02 Meso Scale Technology Graphitic nanotubes in luminescence assays
US5567596A (en) 1994-12-29 1996-10-22 Research Foundation Of State University Of New York Rapid assay of activators and inhibitors of clotting
US5489988A (en) * 1995-01-03 1996-02-06 Motorola Environmental sensor and method therefor
AU4213396A (en) 1995-01-26 1996-08-01 Nippon Paint Co., Ltd. Kit for immunologically assaying biological substance and assay process
US5569608A (en) 1995-01-30 1996-10-29 Bayer Corporation Quantitative detection of analytes on immunochromatographic strips
FR2730810B1 (fr) 1995-02-21 1997-03-14 Thomson Csf Capteur chimique hautement selectif
AU4927496A (en) * 1995-02-21 1996-09-11 Iqbal W. Siddiqi Apparatus and method for mixing and separation employing magnetic particles
AU6378696A (en) 1995-06-05 1996-12-24 Kimberly-Clark Worldwide, Inc. Novel pre-dyes
ATE358276T1 (de) 1995-06-07 2007-04-15 Bioveris Corp Elektrochemilumineszenz-enzymimmunoassay
US5518689A (en) * 1995-09-05 1996-05-21 Bayer Corporation Diffused light reflectance readhead
AUPN527995A0 (en) 1995-09-07 1995-09-28 Agen Biomedical Limited Method and apparatus for semiquantification of an analyte
CA2245664A1 (en) * 1996-02-08 1997-08-14 Australian Membrane And Biotechnology Research Institute Enzyme detection biosensors
EP0890093A4 (en) 1996-03-19 2000-03-15 Univ Utah Res Found SYSTEM FOR DETERMINING THE CONCENTRATION OF A SAMPLE TO BE ANALYZED
US5753517A (en) * 1996-03-29 1998-05-19 University Of British Columbia Quantitative immunochromatographic assays
DE69705938D1 (de) * 1996-03-29 2001-09-06 Univ British Columbia Bestimmung der plättchenzahl unter verwendung eines plättchen-körnchenproteins
US6048352A (en) 1996-05-17 2000-04-11 Mercury Diagnostics, Inc. Disposable element for use in a body fluid sampling device
US5951492A (en) 1996-05-17 1999-09-14 Mercury Diagnostics, Inc. Methods and apparatus for sampling and analyzing body fluid
DE19621133A1 (de) * 1996-05-24 1997-11-27 Boehringer Mannheim Gmbh Bestimmungsverfahren mit oligomerisierten Rezeptoren
DE19622458C2 (de) * 1996-05-24 1998-03-26 Senslab Ges Zur Entwicklung Un Enzymatisch-elektrochemischer Einschritt-Affinitätssensor zur quantitativen Bestimmung von Analyten in wäßrigen Medien und Affinitätsassay
US5876944A (en) 1996-06-10 1999-03-02 Bayer Corporation Method for amplification of the response signal in a sandwich immunoassay
US6556299B1 (en) * 1996-07-10 2003-04-29 Packard Instrument Company, Inc. Imaging system for fluorescence assays
US5660790A (en) 1996-08-13 1997-08-26 Litmus Concepts, Inc. PH and amine test elements
US6020047A (en) * 1996-09-04 2000-02-01 Kimberly-Clark Worldwide, Inc. Polymer films having a printed self-assembling monolayer
US5798273A (en) 1996-09-25 1998-08-25 Becton Dickinson And Company Direct read lateral flow assay for small analytes
US6194220B1 (en) * 1996-09-25 2001-02-27 Becton, Dickinson And Company Non-instrumented assay with quantitative and qualitative results
US6165798A (en) 1996-10-10 2000-12-26 University Of British Columbia Optical quantification of analytes in membranes
US5922537A (en) 1996-11-08 1999-07-13 N.o slashed.AB Immunoassay, Inc. Nanoparticles biosensor
US6048623A (en) * 1996-12-18 2000-04-11 Kimberly-Clark Worldwide, Inc. Method of contact printing on gold coated films
US5827748A (en) 1997-01-24 1998-10-27 The United States Of America As Represented By The Secretary Of The Navy Chemical sensor using two-dimensional lens array
EP0859230A1 (en) 1997-02-10 1998-08-19 Cranfield University Detection of analytes using electrochemistry
US6180288B1 (en) * 1997-03-21 2001-01-30 Kimberly-Clark Worldwide, Inc. Gel sensors and method of use thereof
US6235471B1 (en) * 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6103536A (en) * 1997-05-02 2000-08-15 Silver Lake Research Corporation Internally referenced competitive assays
US6171780B1 (en) * 1997-06-02 2001-01-09 Aurora Biosciences Corporation Low fluorescence assay platforms and related methods for drug discovery
US6136611A (en) 1997-07-31 2000-10-24 Research International, Inc. Assay methods and apparatus
EP0898169B1 (en) 1997-08-11 2002-02-06 F. Hoffmann-La Roche Ag Microparticle enhanced light scattering assay and microparticle reagents therefor
US6080391A (en) 1997-08-14 2000-06-27 Novo Nordisk A/S Reduction of malodour
TR200000565T2 (tr) 1997-08-29 2000-07-21 Fertilitiy Acoustics Inc. Biyolojik örneklerdeki analiz bileşenlerinin hızlı analizi için yöntem
US5872261A (en) * 1997-09-18 1999-02-16 Pierce Chemical Company Preparation of sulfo-N- hydroxy succinimide salts with intermediate formation of diester
US5906921A (en) * 1997-09-29 1999-05-25 Matsushita Electric Industrial Co., Ltd. Biosensor and method for quantitative measurement of a substrate using the same
WO1999018438A1 (en) 1997-10-02 1999-04-15 Aclara Biosciences, Inc. Capillary assays involving separation of free and bound species
US6174646B1 (en) * 1997-10-21 2001-01-16 Konica Corporation Image forming method
US6030792A (en) * 1997-11-13 2000-02-29 Pfizer Inc Assays for measurement of protein fragments in biological media
US6306642B1 (en) 1997-11-24 2001-10-23 Quidel Corporation Enzyme substrate delivery and product registration in one step enzyme immunoassays
US6074725A (en) * 1997-12-10 2000-06-13 Caliper Technologies Corp. Fabrication of microfluidic circuits by printing techniques
EP1046027A1 (en) 1997-12-11 2000-10-25 Quidel Corporation One-step fluorescent immunosensor test
US6060256A (en) * 1997-12-16 2000-05-09 Kimberly-Clark Worldwide, Inc. Optical diffraction biosensor
SE9704933D0 (sv) 1997-12-30 1997-12-30 Pharmacia & Upjohn Diag Ab Metod som utnyttjar en ny kalibrator och test kit som innehåller kalibratorn
DE19811622A1 (de) 1998-03-17 1999-09-23 Lre Technology Partner Gmbh Meßgerät zur Bestimmung der Konzentration einer Substanz in einer Flüssigkeit
US6017723A (en) * 1998-03-27 2000-01-25 Long Island Jewish Medical Center Method for isolating inhibitors of protease activity
US6368873B1 (en) * 1998-04-09 2002-04-09 Applied Biotech, Inc. Identification of human urine for drug testing
US6241863B1 (en) 1998-04-27 2001-06-05 Harold G. Monbouquette Amperometric biosensors based on redox enzymes
US6451607B1 (en) 1998-05-07 2002-09-17 Litmus Concepts, Inc. External dried-reagent control for analytical test devices
WO1999064864A1 (en) 1998-06-12 1999-12-16 New Horizons Diagnostics Inc. Optimizing sensitivity in colloidal colorimetric flow through and lateral flow tests
US6030840A (en) * 1998-06-15 2000-02-29 Nen Life Sciences, Inc. Neutral enhancement of lanthanides for time resolved fluorescence
US6183972B1 (en) * 1998-07-27 2001-02-06 Bayer Corporation Method for the determination of analyte concentration in a lateral flow sandwich immunoassay exhibiting high-dose hook effect
US6171870B1 (en) * 1998-08-06 2001-01-09 Spectral Diagnostics, Inc. Analytical test device and method for use in medical diagnoses
WO2000019199A1 (en) 1998-09-29 2000-04-06 Fertility Acoustics Inc. A method of and device for determining ovulation in mammals
US6284472B1 (en) 1998-10-05 2001-09-04 Dade Behring Inc. Method for extending the range of an immunoassay
US6338790B1 (en) 1998-10-08 2002-01-15 Therasense, Inc. Small volume in vitro analyte sensor with diffusible or non-leachable redox mediator
BE1012241A3 (fr) 1998-10-21 2000-08-01 D Tek Procede de depistage d'analyte et trousse pour la mise en oeuvre d'un tel procede.
US6455861B1 (en) 1998-11-24 2002-09-24 Cambridge Research & Instrumentation, Inc. Fluorescence polarization assay system and method
US6221579B1 (en) 1998-12-11 2001-04-24 Kimberly-Clark Worldwide, Inc. Patterned binding of functionalized microspheres for optical diffraction-based biosensors
US6579673B2 (en) 1998-12-17 2003-06-17 Kimberly-Clark Worldwide, Inc. Patterned deposition of antibody binding protein for optical diffraction-based biosensors
US5981207A (en) 1998-12-18 1999-11-09 Pharmacopeia, Inc. Caged enzyme substrates as probes for reporter enzyme activity
CA2364288A1 (en) 1999-02-11 2000-08-17 Robert D. Macphee Enzyme-linked immuno-magnetic electrochemical biosensor
EP1163513A1 (en) 1999-02-26 2001-12-19 Fertility Acoustics Inc. Analyzing strip having a fluid cell and a method of analyzing a sample
US6511814B1 (en) * 1999-03-26 2003-01-28 Idexx Laboratories, Inc. Method and device for detecting analytes in fluids
WO2000078917A1 (en) 1999-06-18 2000-12-28 Umedik, Inc. Device and method for analyzing a biologic sample
US6294392B1 (en) 1999-07-21 2001-09-25 The Regents Of The University Of California Spatially-encoded analyte detection
US6372895B1 (en) 2000-07-07 2002-04-16 3M Innovative Properties Company Fluorogenic compounds
CA2385613C (en) 1999-09-29 2005-12-06 Japan Science And Technology Corporation High sensitivity immunoassay method
US6136549A (en) 1999-10-15 2000-10-24 Feistel; Christopher C. systems and methods for performing magnetic chromatography assays
AU1241901A (en) 1999-11-24 2001-06-04 Biotronics Technologies, Inc. Devices and methods for detecting analytes using electrosensor having capture reagent
US6399295B1 (en) 1999-12-17 2002-06-04 Kimberly-Clark Worldwide, Inc. Use of wicking agent to eliminate wash steps for optical diffraction-based biosensors
US6485926B2 (en) 1999-12-22 2002-11-26 Fuji Photo Film Co., Ltd. Method for measuring protease activity
US6509196B1 (en) * 2000-01-04 2003-01-21 Response Biomedical Corp. Compensation for non-specific signals in quantitative immunoassays
US6255066B1 (en) 2000-02-08 2001-07-03 Allan L. Louderback Bacterial vaginosis screening technique and a diagnostic kit for use therein
CA2437298A1 (en) 2000-02-23 2001-08-30 Besst-Test Aps Method for correlating blood coagulation activity with markers in body fluids, e.g. urine
US6607922B2 (en) 2000-03-17 2003-08-19 Quantum Design, Inc. Immunochromatographic assay method and apparatus
US6436722B1 (en) 2000-04-18 2002-08-20 Idexx Laboratories, Inc. Device and method for integrated diagnostics with multiple independent flow paths
US20050032051A1 (en) 2000-06-19 2005-02-10 Hayes Mark A Rapid flow-based immunoassay microchip
EP1311839B1 (en) 2000-06-21 2006-03-01 Bioarray Solutions Ltd Multianalyte molecular analysis using application-specific random particle arrays
US6528321B1 (en) * 2000-06-26 2003-03-04 Beckman Coulter, Inc. Opposable-element chromatographic assay device for detection of analytes in whole blood samples
WO2002033396A1 (en) * 2000-10-14 2002-04-25 Saunders Alex M Ligand based solution assay for low concentration analytes
US6720007B2 (en) 2000-10-25 2004-04-13 Tufts University Polymeric microspheres
US20020164659A1 (en) 2000-11-30 2002-11-07 Rao Galla Chandra Analytical methods and compositions
US20030162236A1 (en) 2001-03-26 2003-08-28 Response Biomedical Corporation Compensation for variability in specific binding in quantitative assays
JP2002303629A (ja) 2001-04-06 2002-10-18 Matsushita Electric Ind Co Ltd 免疫クロマトデバイス及びそれを用いた被検物質測定方法
US6818456B2 (en) 2001-07-20 2004-11-16 Varian, Inc. Color contrast system for lateral flow immunoassay tests
EP1425585A2 (en) 2001-09-11 2004-06-09 MERCK PATENT GmbH Lateral flow test format for enzyme assays
US20030119073A1 (en) 2001-12-21 2003-06-26 Stephen Quirk Sensors and methods of detection for proteinase enzymes
US8367013B2 (en) 2001-12-24 2013-02-05 Kimberly-Clark Worldwide, Inc. Reading device, method, and system for conducting lateral flow assays
US20030119203A1 (en) 2001-12-24 2003-06-26 Kimberly-Clark Worldwide, Inc. Lateral flow assay devices and methods for conducting assays
US20040029205A1 (en) 2002-07-30 2004-02-12 Small Parker Adams Diagnostic system for differentiating sputum from saliva
US7285424B2 (en) 2002-08-27 2007-10-23 Kimberly-Clark Worldwide, Inc. Membrane-based assay devices
US7314763B2 (en) 2002-08-27 2008-01-01 Kimberly-Clark Worldwide, Inc. Fluidics-based assay devices
US7432105B2 (en) 2002-08-27 2008-10-07 Kimberly-Clark Worldwide, Inc. Self-calibration system for a magnetic binding assay
US7041469B2 (en) 2002-10-10 2006-05-09 Quidel Corporation Assays for trichomonal and other hydrolases
US7781172B2 (en) 2003-11-21 2010-08-24 Kimberly-Clark Worldwide, Inc. Method for extending the dynamic detection range of assay devices
US20040106190A1 (en) 2002-12-03 2004-06-03 Kimberly-Clark Worldwide, Inc. Flow-through assay devices
EP1704245A1 (en) 2003-12-31 2006-09-27 Applera Corporation Multiplex protease profiling
GB2422664A (en) 2005-01-28 2006-08-02 Ethicon Inc Device for detecting an enzyme in a sample
US20050191704A1 (en) * 2004-03-01 2005-09-01 Kimberly-Clark Worldwide, Inc. Assay devices utilizing chemichromic dyes
US7906276B2 (en) * 2004-06-30 2011-03-15 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
US7094528B2 (en) 2004-06-30 2006-08-22 Kimberly-Clark Worldwide, Inc. Magnetic enzyme detection techniques
GB2435511A (en) 2006-02-23 2007-08-29 Mologic Ltd Protease detection
GB2437311A (en) 2006-04-07 2007-10-24 Mologic Ltd A protease detection product

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0244932A2 (en) * 1986-03-10 1987-11-11 Board Of Regents, The University Of Texas System Solid-phase substrate yielding soluble labeled products upon hydrolysis by a glycosaminoglycan endoglycosidase
US5124254A (en) * 1988-02-08 1992-06-23 University College Cardiff Consultants Limited Detection of diamines in biological fluids
US5292652A (en) * 1990-10-05 1994-03-08 Athena Neurosciences, Inc. Amyloidin protease and uses thereof
US5585273A (en) * 1993-04-14 1996-12-17 Litmus Concepts, Inc. Test device for assays for hydrolytic enzyme activity
US6451619B1 (en) * 1994-06-29 2002-09-17 Inverness Medical Switzerland Gmbh Monitoring methods and devices for use therein
US6243980B1 (en) * 1997-03-07 2001-06-12 Tropix, Inc. Protease inhibitor assay
WO2003085403A1 (en) * 2002-03-30 2003-10-16 Xenotope Diagnostics, Inc. Method and device for trichomonas detection
US20040121480A1 (en) * 2002-12-19 2004-06-24 Kimberly-Clark Worldwide, Inc. Reduction of the hook effect in membrane-based assay devices

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1920253A2 (en) * 2005-08-31 2008-05-14 Kimberly-Clark Worldwide, Inc. Detection of proteases secreted from pathogenic microorganisms

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