WO2005080958A1 - Procédé de détection d'adn à double brin présentant une séquence spécifique - Google Patents

Procédé de détection d'adn à double brin présentant une séquence spécifique Download PDF

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Publication number
WO2005080958A1
WO2005080958A1 PCT/JP2005/002687 JP2005002687W WO2005080958A1 WO 2005080958 A1 WO2005080958 A1 WO 2005080958A1 JP 2005002687 W JP2005002687 W JP 2005002687W WO 2005080958 A1 WO2005080958 A1 WO 2005080958A1
Authority
WO
WIPO (PCT)
Prior art keywords
double
stranded dna
dna
electrophoresis
polymer gel
Prior art date
Application number
PCT/JP2005/002687
Other languages
English (en)
Japanese (ja)
Inventor
Norihiko Minoura
Masayo Ogiso
Original Assignee
National Institute Of Advanced Industrial Science And Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Institute Of Advanced Industrial Science And Technology filed Critical National Institute Of Advanced Industrial Science And Technology
Priority to JP2006510260A priority Critical patent/JP4423397B2/ja
Publication of WO2005080958A1 publication Critical patent/WO2005080958A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

Abstract

Un échantillon contenant de l'ADN à double brin subit une électrophorèse en utilisant un gel de polymère qui comporte un pore présentant un modèle sous la forme d'une molécule d'ADN à double brin imprégné sur celui-ci et présentant en outre un substituant reconnaissant une paire de base de l'ADN à double brin dans le pore en tant que milieu d'électrophorèse. Ainsi, l'ADN à double brin peut être facilement et rapidement détecté et/ou séparé dans l'état de double brin en tant que tel.
PCT/JP2005/002687 2004-02-23 2005-02-21 Procédé de détection d'adn à double brin présentant une séquence spécifique WO2005080958A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2006510260A JP4423397B2 (ja) 2004-02-23 2005-02-21 特異的配列をもつニ本鎖ｄｎａを検出する方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004046474 2004-02-23
JP2004-046474 2004-02-23

Publications (1)

Publication Number Publication Date
WO2005080958A1 true WO2005080958A1 (fr) 2005-09-01

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ID=34879443

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2005/002687 WO2005080958A1 (fr) 2004-02-23 2005-02-21 Procédé de détection d'adn à double brin présentant une séquence spécifique

Country Status (2)

Country Link
JP (1) JP4423397B2 (fr)
WO (1) WO2005080958A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007093230A (ja) * 2005-09-27 2007-04-12 National Institute Of Advanced Industrial & Technology 標的ｄｎａ解析方法及び解析装置

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001019886A1 (fr) * 1999-09-17 2001-03-22 Mip Technologies Ab Nouveaux polymeres a empreinte moleculaire greffes sur des supports solides

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001019886A1 (fr) * 1999-09-17 2001-03-22 Mip Technologies Ab Nouveaux polymeres a empreinte moleculaire greffes sur des supports solides

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
HAUPT K. ET AL.: "Molecularly imprinted polymers in analytical chemistry.", ANALYST., 2001, pages 747 - 756, XP002997699 *
HUANG Y. ET AL.: "Preparation and evaluation of molecularly imprinted polymers based on 9-ethladenine for the recognition of nucleotide bases in capillary electrochromatography.", ELECTROPHORESIS, vol. 25, no. 4-5, February 2004 (2004-02-01), pages 554 - 561, XP002997700 *
MAYES A. ET AL.: "Molecularly imprinted polymers: useful materials for analytical chemistry?", TRENSDS IN ANALYTICAL CHEMISTRY., vol. 16, no. 6, 1997, pages 321 - 332, XP004086340 *
MINOURA N. ET AL.: "DNA Imprinting Polymer o Mochiita Nihonsa DNA Kenshutsu Hoho no Kaihatsu.", MEMBRANE SYMPOSIUM (KYOTO), no. 16, 1 November 2004 (2004-11-01), pages 125 - 128, XP002998403 *
MINOURA N. ET AL.: "DNA Sentakuteki Ninshiki Hyomen o Motsu Usumaku no Keisei Maku Symposium.", MEMBRANE SYMPOSIUM (KYOTO), no. 14, 1 November 2002 (2002-11-01), pages 37 - 40, XP002998401 *
MINOURA N. ET AL: "Nihonsa DNA o Ninshiki suru Polymer Gel no Gosei to Soreo Riyo shita DNA no Kenshutsu.", DAI 42 KAI KOBUNSHI TO MIZU NI KANSURU TORONKAI KOEN YOSHISHU., 3 December 2004 (2004-12-03), pages 17 - 18, XP002998404 *
OGISO M ET AL.: "Molecularly Imprinted Polymer for Recognizing Double-stranded DNA.", CHEMICAL SENSORS., vol. 20, 11 July 2004 (2004-07-11), pages 494 - 495, XP002998402 *
OGISO M. ET AL.: "DNA Sentakuteki Ninshiki Hyomen p Motsu Bunshi Imprinting Polymer Gel no Tokusei.", DAI 41 KAI KOBUNSHI TO MIZU NI KANSURU TORONKAI KOEN YOSHISHU, 12 December 2003 (2003-12-12), pages 11 - 12, XP002997698 *
TAKEUCHI T. ET AL.: "Separation and sensing based on moleculr recognition using molecularly imprinted polymers.", CHROMATOGRAPHY B., vol. 728, no. 1, 14 May 1999 (1999-05-14), pages 1 - 20, XP004166183 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007093230A (ja) * 2005-09-27 2007-04-12 National Institute Of Advanced Industrial & Technology 標的ｄｎａ解析方法及び解析装置

Also Published As

Publication number Publication date
JPWO2005080958A1 (ja) 2007-10-25
JP4423397B2 (ja) 2010-03-03

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