WO2005035755A1 - 核酸導入方法 - Google Patents
核酸導入方法 Download PDFInfo
- Publication number
- WO2005035755A1 WO2005035755A1 PCT/JP2004/012004 JP2004012004W WO2005035755A1 WO 2005035755 A1 WO2005035755 A1 WO 2005035755A1 JP 2004012004 W JP2004012004 W JP 2004012004W WO 2005035755 A1 WO2005035755 A1 WO 2005035755A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- electrode substrate
- cationic
- nucleic acid
- cells
- substrate
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Nanotechnology (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Composite Materials (AREA)
- Crystallography & Structural Chemistry (AREA)
- Materials Engineering (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Electromagnetism (AREA)
- Sustainable Development (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE602004023631T DE602004023631D1 (de) | 2003-10-08 | 2004-08-20 | Verfahren zur einführung einer nukleinsäure |
JP2005514533A JP4534043B2 (ja) | 2003-10-08 | 2004-08-20 | 核酸導入方法 |
US10/572,920 US7846731B2 (en) | 2003-10-08 | 2004-08-20 | Method of introducing nucelic acid |
AU2004280029A AU2004280029B2 (en) | 2003-10-08 | 2004-08-20 | Method of introducing nucleic acid |
EP04771965A EP1672063B1 (en) | 2003-10-08 | 2004-08-20 | Method of introducing nucleic acid |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2003-349343 | 2003-10-08 | ||
JP2003349343 | 2003-10-08 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2005035755A1 true WO2005035755A1 (ja) | 2005-04-21 |
Family
ID=34431002
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2004/012004 WO2005035755A1 (ja) | 2003-10-08 | 2004-08-20 | 核酸導入方法 |
Country Status (7)
Country | Link |
---|---|
US (1) | US7846731B2 (ja) |
EP (1) | EP1672063B1 (ja) |
JP (1) | JP4534043B2 (ja) |
CN (1) | CN1863910A (ja) |
AU (1) | AU2004280029B2 (ja) |
DE (1) | DE602004023631D1 (ja) |
WO (1) | WO2005035755A1 (ja) |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006197872A (ja) * | 2005-01-21 | 2006-08-03 | Tohoku Univ | 電気穿孔法および電気穿孔用キュベット |
WO2008102728A1 (ja) | 2007-02-19 | 2008-08-28 | Kyoto University | 核酸導入用導電性基板および核酸導入方法 |
JP2011518561A (ja) * | 2008-04-24 | 2011-06-30 | バイオ−ラッド ラボラトリーズ,インコーポレイティド | 付着細胞のエレクトロポレーションのためのディスク表面の使用 |
JP2013516163A (ja) * | 2009-12-30 | 2013-05-13 | アイメック | 改良マイクロニードル |
JP2014131014A (ja) * | 2012-11-28 | 2014-07-10 | Shin Etsu Chem Co Ltd | 透明酸化物電極用表面修飾剤、表面修飾された透明酸化物電極、及び表面修飾された透明酸化物電極の製造方法 |
JP2018134102A (ja) * | 2007-12-14 | 2018-08-30 | ビオエヌテヒ・アクチエンゲゼルシャフト | 体細胞を再プログラムするためのrnaの使用 |
EP3459588A1 (en) | 2017-09-20 | 2019-03-27 | L'oreal | Electrical method of delivering hyaluronic acid through the skin |
JP2019118291A (ja) * | 2017-12-28 | 2019-07-22 | 株式会社ベックス | エレクトロポレーション用チャンバー及びチャンバーホルダー |
WO2019185168A1 (en) | 2018-03-30 | 2019-10-03 | L'oreal | Electroporation of hyaluronic acid and heating |
KR20210017238A (ko) * | 2019-08-07 | 2021-02-17 | 엘지전자 주식회사 | 전기천공 장치용 챔버 |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008104086A1 (en) | 2007-03-01 | 2008-09-04 | Queen's University At Kingston | Planar electroporation apparatus and method |
US20100168202A1 (en) * | 2007-06-06 | 2010-07-01 | Lieberman Howard B | Rad 9 as a diagnostic,prognostic,and therapeutic tool for prostate cancer |
CN101955973B (zh) * | 2009-07-14 | 2012-11-07 | 复旦大学附属眼耳鼻喉科医院 | 一种利用电穿孔辅助基因转移的方法及装置 |
KR101362076B1 (ko) * | 2010-08-06 | 2014-02-12 | 명지대학교 산학협력단 | 주사 탐침 현미경을 이용하는 전기 천공법 및 전기 천공 장치 |
CN102367414B (zh) * | 2011-06-04 | 2014-04-09 | 苏州文曲生物微系统有限公司 | 一种用于向活体组织中的细胞内部输运核酸的柔性电极芯片 |
US9382510B2 (en) | 2011-08-25 | 2016-07-05 | Jian Chen | Methods and devices for electroporation |
WO2013167185A1 (en) | 2012-05-09 | 2013-11-14 | Universitat Politècnica De Catalunya | Electrode assembly for generating electric field pulses to perform electroporation to a biological sample |
KR102036623B1 (ko) * | 2013-02-20 | 2019-11-26 | 지엔 첸 | 전기천공 방법 및 장치 |
CN108251268A (zh) * | 2016-12-29 | 2018-07-06 | 上海新微技术研发中心有限公司 | 一种基因转染器及基因转染方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0594282A2 (en) * | 1992-07-29 | 1994-04-27 | Director General Of Fruit Tree Research Station Of Japan, Ministry Of Agriculture, Forestry And Fisheries | Electrode for electrical cell fusion and electrical introduction of nucleic acids |
JPH11103858A (ja) * | 1997-10-01 | 1999-04-20 | Tr Tec Kk | エレクトロポレーションによるdna導入方法 |
JP2003075402A (ja) * | 2001-09-03 | 2003-03-12 | Fuji Photo Film Co Ltd | 核酸断片固定電極及びその利用 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4441972A (en) * | 1983-04-08 | 1984-04-10 | D.E.P. Systems, Inc. | Apparatus for electrofusion of biological particles |
CA2019758C (en) * | 1990-06-25 | 2001-09-04 | Kevin L. Firth | Improved electroporation device and method |
-
2004
- 2004-08-20 JP JP2005514533A patent/JP4534043B2/ja active Active
- 2004-08-20 WO PCT/JP2004/012004 patent/WO2005035755A1/ja active Application Filing
- 2004-08-20 US US10/572,920 patent/US7846731B2/en not_active Expired - Fee Related
- 2004-08-20 EP EP04771965A patent/EP1672063B1/en not_active Not-in-force
- 2004-08-20 CN CN200480029594.3A patent/CN1863910A/zh active Pending
- 2004-08-20 AU AU2004280029A patent/AU2004280029B2/en not_active Expired - Fee Related
- 2004-08-20 DE DE602004023631T patent/DE602004023631D1/de active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0594282A2 (en) * | 1992-07-29 | 1994-04-27 | Director General Of Fruit Tree Research Station Of Japan, Ministry Of Agriculture, Forestry And Fisheries | Electrode for electrical cell fusion and electrical introduction of nucleic acids |
JPH11103858A (ja) * | 1997-10-01 | 1999-04-20 | Tr Tec Kk | エレクトロポレーションによるdna導入方法 |
JP2003075402A (ja) * | 2001-09-03 | 2003-03-12 | Fuji Photo Film Co Ltd | 核酸断片固定電極及びその利用 |
Non-Patent Citations (4)
Title |
---|
See also references of EP1672063A4 * |
WU, R.Z ET AL: "CELL-BIOLOGICAL APPLICATIONS OF TRANSFECTED-CELL MICROARRAYS", TRENDS IN CELL BIOLOGY, vol. 12, no. 10, 2002, pages 485 - 488, XP001184158 * |
YAMAUCHI F. ET AL: "MICROPATTERNED, SELF-ASSEMBLED MONOLAYERS FOR FABRICATION OF TRANSFECTED CELL MICROARRAYS", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1672, no. 3, 11 June 2004 (2004-06-11), pages 138 - 147, XP004514833 * |
ZLAUDDIN J. ET AL: "MICROARRAYS OF CELLS EXPRESSING DEFINED cDNAS", NATURE, vol. 411, no. 6833, 2001, pages 107 - 110, XP002246363 * |
Cited By (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006197872A (ja) * | 2005-01-21 | 2006-08-03 | Tohoku Univ | 電気穿孔法および電気穿孔用キュベット |
WO2008102728A1 (ja) | 2007-02-19 | 2008-08-28 | Kyoto University | 核酸導入用導電性基板および核酸導入方法 |
JPWO2008102728A1 (ja) * | 2007-02-19 | 2010-05-27 | 国立大学法人京都大学 | 核酸導入用導電性基板および核酸導入方法 |
JP2018134102A (ja) * | 2007-12-14 | 2018-08-30 | ビオエヌテヒ・アクチエンゲゼルシャフト | 体細胞を再プログラムするためのrnaの使用 |
US11008550B2 (en) | 2007-12-14 | 2021-05-18 | BioNTech SE | Use of RNA for reprogramming somatic cells |
JP2011518561A (ja) * | 2008-04-24 | 2011-06-30 | バイオ−ラッド ラボラトリーズ,インコーポレイティド | 付着細胞のエレクトロポレーションのためのディスク表面の使用 |
JP2013516163A (ja) * | 2009-12-30 | 2013-05-13 | アイメック | 改良マイクロニードル |
JP2014131014A (ja) * | 2012-11-28 | 2014-07-10 | Shin Etsu Chem Co Ltd | 透明酸化物電極用表面修飾剤、表面修飾された透明酸化物電極、及び表面修飾された透明酸化物電極の製造方法 |
EP3459588A1 (en) | 2017-09-20 | 2019-03-27 | L'oreal | Electrical method of delivering hyaluronic acid through the skin |
WO2019057511A2 (en) | 2017-09-20 | 2019-03-28 | L'oreal | ELECTRICAL METHOD OF DELIVERING HYALURONIC ACID THROUGH THE SKIN |
JP2019118291A (ja) * | 2017-12-28 | 2019-07-22 | 株式会社ベックス | エレクトロポレーション用チャンバー及びチャンバーホルダー |
WO2019185168A1 (en) | 2018-03-30 | 2019-10-03 | L'oreal | Electroporation of hyaluronic acid and heating |
KR20210017238A (ko) * | 2019-08-07 | 2021-02-17 | 엘지전자 주식회사 | 전기천공 장치용 챔버 |
KR102348640B1 (ko) | 2019-08-07 | 2022-01-07 | 엘지전자 주식회사 | 전기천공 장치 |
Also Published As
Publication number | Publication date |
---|---|
DE602004023631D1 (de) | 2009-11-26 |
US20070059832A1 (en) | 2007-03-15 |
EP1672063A4 (en) | 2006-12-13 |
US7846731B2 (en) | 2010-12-07 |
AU2004280029B2 (en) | 2009-02-26 |
EP1672063A1 (en) | 2006-06-21 |
JPWO2005035755A1 (ja) | 2006-12-21 |
EP1672063B1 (en) | 2009-10-14 |
CN1863910A (zh) | 2006-11-15 |
AU2004280029A1 (en) | 2005-04-21 |
JP4534043B2 (ja) | 2010-09-01 |
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