WO2008102728A1 - 核酸導入用導電性基板および核酸導入方法 - Google Patents

核酸導入用導電性基板および核酸導入方法 Download PDF

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Publication number
WO2008102728A1
WO2008102728A1 PCT/JP2008/052658 JP2008052658W WO2008102728A1 WO 2008102728 A1 WO2008102728 A1 WO 2008102728A1 JP 2008052658 W JP2008052658 W JP 2008052658W WO 2008102728 A1 WO2008102728 A1 WO 2008102728A1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
conductive substrate
delivering
delivery
acid delivery
Prior art date
Application number
PCT/JP2008/052658
Other languages
English (en)
French (fr)
Inventor
Hiroo Iwata
Yuuki Inoue
Original Assignee
Kyoto University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyoto University filed Critical Kyoto University
Priority to EP08711482A priority Critical patent/EP2113565A4/en
Priority to US12/526,487 priority patent/US20110091974A1/en
Priority to JP2009500171A priority patent/JPWO2008102728A1/ja
Publication of WO2008102728A1 publication Critical patent/WO2008102728A1/ja

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Biophysics (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

細胞への核酸導入用導電性基板であって、カチオン性表面電極基板に、カルボキシル基を有するカーボンナノチューブおよび核酸が担持されてなることを特徴とする核酸導入用導電性基板。
PCT/JP2008/052658 2007-02-19 2008-02-18 核酸導入用導電性基板および核酸導入方法 WO2008102728A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP08711482A EP2113565A4 (en) 2007-02-19 2008-02-18 CONDUCTIVE SUBSTRATE FOR NUCLEIC ACID RELIEF AND NUCLEIC ACID RELIEF PROCESS
US12/526,487 US20110091974A1 (en) 2007-02-19 2008-02-18 Conductive Substrate and Method for Introducing Nucleic Acid
JP2009500171A JPWO2008102728A1 (ja) 2007-02-19 2008-02-18 核酸導入用導電性基板および核酸導入方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007038067 2007-02-19
JP2007-038067 2007-02-19

Publications (1)

Publication Number Publication Date
WO2008102728A1 true WO2008102728A1 (ja) 2008-08-28

Family

ID=39710005

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/052658 WO2008102728A1 (ja) 2007-02-19 2008-02-18 核酸導入用導電性基板および核酸導入方法

Country Status (4)

Country Link
US (1) US20110091974A1 (ja)
EP (1) EP2113565A4 (ja)
JP (1) JPWO2008102728A1 (ja)
WO (1) WO2008102728A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012003466A1 (en) * 2010-07-02 2012-01-05 Ensysce Biosciences, Inc. Single-walled carbon nanotube/sirna complexes and methods related thereto
WO2014126979A1 (en) 2013-02-14 2014-08-21 Novartis Ag Substituted bisphenyl butanoic phosphonic acid derivatives as nep (neutral endopeptidase) inhibitors

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2911034T3 (es) 2006-08-08 2022-05-17 Univ Bonn Rheinische Friedrich Wilhelms Estructura y uso de oligonucleótidos 5' fosfato
US9738680B2 (en) 2008-05-21 2017-08-22 Rheinische Friedrich-Wilhelms-Universität Bonn 5′ triphosphate oligonucleotide with blunt end and uses thereof
FR2952648B1 (fr) * 2009-11-17 2013-05-03 Thales Sa Procede de poration de cellules comprises dans un substrat comportant l'utilisation de nanotubes comme recepteur hyperfrequences
EP2508530A1 (en) 2011-03-28 2012-10-10 Rheinische Friedrich-Wilhelms-Universität Bonn Purification of triphosphorylated oligonucleotides using capture tags
EP2712870A1 (en) 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Novel RIG-I ligands and methods for producing them
CA2901759C (en) * 2013-02-20 2021-01-12 Jian Chen Methods and devices for electroporation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070875A2 (de) * 2002-02-20 2003-08-28 Amaxa Gmbh Behälter mit zumindest einer elektrode
WO2005035755A1 (ja) 2003-10-08 2005-04-21 Kyoto University 核酸導入方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7850645B2 (en) * 2005-02-11 2010-12-14 Boston Scientific Scimed, Inc. Internal medical devices for delivery of therapeutic agent in conjunction with a source of electrical power

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070875A2 (de) * 2002-02-20 2003-08-28 Amaxa Gmbh Behälter mit zumindest einer elektrode
WO2005035755A1 (ja) 2003-10-08 2005-04-21 Kyoto University 核酸導入方法

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
CAI D. ET AL.: "Highly efficient molecular delivery into mammalian cells using carbon nanotube spearing", NATURE METHODS, vol. 2, no. 6, 2005, pages 449 - 454, XP008112005 *
F. YAMAUCHI; K. KATO; H. IWATA, NUCLEIC ACIDS RES., vol. 32, 2004, pages E187
J. LIU ET AL., SCIENCE, vol. 280, 1998, pages 1253 - 1256
J. ZIAUDDIN; D. M. SABATINI, NATURE, vol. 411, 2001, pages 107 - 110
ROJAS-CHAPANA J. ET AL.: "Multi-walled carbon nanotubes for plasmid delivery into Escherichia coli cells", LAB. ON A CHIP, vol. 5, 2005, pages 536 - 539, XP008112007 *
See also references of EP2113565A4 *
YAMAUCHI F. ET AL.: "Layer-by-layer assembly of cationic lipid and plasmid DNA onto gold surface for stent-assisted gene transfer", BIOMATERIALS, vol. 27, 2006, pages 3497 - 3504, XP005341728 *
YAMAUCHI F. ET AL.: "Layer-by-Layer assembly of poly(ethyleneimine) and plasmid DNA onto transparent Indium-Tin oxide electrodes for temporally and spatially specific gene transfer", LANGMUIR, vol. 21, no. 18, 2005, pages 8360 - 8367, XP002404718 *
YAMAUCHI F. ET AL.: "Spatially and temporally controlled gene transfer by electroporation into adherent cells on plasmid DNA-loaded electrodes", NUCLEIC ACIDS RESEARCH, vol. 32, no. 22, E187, 2004, pages 1 - 8, XP002404717 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012003466A1 (en) * 2010-07-02 2012-01-05 Ensysce Biosciences, Inc. Single-walled carbon nanotube/sirna complexes and methods related thereto
US8772258B2 (en) 2010-07-02 2014-07-08 Ensysce Biosciences, Inc. Single walled carbon nanotube/sirna complexes and methods related thereto
WO2014126979A1 (en) 2013-02-14 2014-08-21 Novartis Ag Substituted bisphenyl butanoic phosphonic acid derivatives as nep (neutral endopeptidase) inhibitors

Also Published As

Publication number Publication date
US20110091974A1 (en) 2011-04-21
JPWO2008102728A1 (ja) 2010-05-27
EP2113565A4 (en) 2010-08-18
EP2113565A1 (en) 2009-11-04

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