WO2004078959A1 - Enzyme de rupture de pullulane, procede d'elaboration et utilisation - Google Patents

Enzyme de rupture de pullulane, procede d'elaboration et utilisation Download PDF

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Publication number
WO2004078959A1
WO2004078959A1 PCT/JP2004/002567 JP2004002567W WO2004078959A1 WO 2004078959 A1 WO2004078959 A1 WO 2004078959A1 JP 2004002567 W JP2004002567 W JP 2004002567W WO 2004078959 A1 WO2004078959 A1 WO 2004078959A1
Authority
WO
WIPO (PCT)
Prior art keywords
pullulan
degrading enzyme
molecular weight
enzyme
producing
Prior art date
Application number
PCT/JP2004/002567
Other languages
English (en)
Japanese (ja)
Inventor
Kazuhisa Mukai
Michio Kubota
Shigeharu Fukuda
Toshio Miyake
Original Assignee
Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo filed Critical Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
Publication of WO2004078959A1 publication Critical patent/WO2004078959A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C12P19/10—Pullulan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)

Definitions

  • the pullulanase is an enzyme that specifically hydrolyzes the 1,6 glucosidic bond in pullulan to finally produce maltotriose, and is composed of maltotriose and maltotriose units when acted on pullulan. Since oligosaccharides are formed relatively quickly, the molecular weight distribution of the obtained pullulan-containing material is wide.
  • the molecular weight distribution width can be represented by a numerical value (MwZMn) obtained by dividing a weight average molecular weight (hereinafter abbreviated as Mw) by a number average molecular weight (hereinafter abbreviated as Mn). The larger the ratio of, the wider the molecular weight distribution width.
  • the ratio of Mw / Mn approaches 1, it means that the molecular weight distribution width is narrower, and when the ratio of MwZMn is 1, it means that the molecular weight is single.
  • M w the hydrolysis of pullulan by Purura kinase
  • M w the ratio of 000 at 1 ⁇ 1 3 ⁇ 4 7 ZM n
  • M w the ratio of 000 at 1 ⁇ 1 3 ⁇ 4 7 ZM n
  • Hydrolysis to pullulan produces a pullulan-containing material with a broad molecular weight distribution with an MwZMn ratio of about 11.15.
  • FIG. 4 is a diagram showing the pH stability of pullulan-degrading enzyme derived from Aureopacidium 'pullulans IFO 6353. BEST MODE FOR CARRYING OUT THE INVENTION
  • a known solid-liquid separation method for example, a precoat filter, a membrane filtration method using a flat membrane or a hollow fiber membrane, a centrifugation method, or the like is appropriately employed.
  • a method for shaking a salting out method of ammonium sulfate, an acetone and alcohol precipitation method, a membrane concentration method using a flat membrane or a hollow membrane, etc. are appropriately adopted.
  • the purification method polyethylene glycol fractionation, ion-exchange column chromatography, water column chromatography, gel filtration chromatography, etc. are appropriately employed, and by appropriately combining one or more of these purification methods, An enzyme showing a single band by electrophoresis can be obtained.
  • the solution and powdered pullulan products and purified pullulan products obtained in this way can be advantageously used in many applications such as foods and drinks, cosmetics, pharmaceuticals, agriculture, forestry, fisheries and livestock products, as well as molecular weight standard reagents. it can.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne une enzyme de rupture de pullulane, et un procédé d'élaboration. On utilise un micro-organisme du genre Aureobasidium Genus à cet effet. L'invention concerne également un procédé de dégradation de pullulane faisant appel à cette enzyme, et un procédé de production de pullulane à viscosité réduite et/ou de rapport Mw/Mn réduit. On peut utiliser cette enzyme pour produire un pullulane à faible viscosité et de rapport Mw/Mn réduit, et également facile à manipuler.
PCT/JP2004/002567 2003-03-04 2004-03-02 Enzyme de rupture de pullulane, procede d'elaboration et utilisation WO2004078959A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003-56474 2003-03-04
JP2003056474A JP2004261132A (ja) 2003-03-04 2003-03-04 プルラン分解酵素とその製造方法並びに用途

Publications (1)

Publication Number Publication Date
WO2004078959A1 true WO2004078959A1 (fr) 2004-09-16

Family

ID=32958708

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2004/002567 WO2004078959A1 (fr) 2003-03-04 2004-03-02 Enzyme de rupture de pullulane, procede d'elaboration et utilisation

Country Status (3)

Country Link
JP (1) JP2004261132A (fr)
TW (1) TW200502393A (fr)
WO (1) WO2004078959A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10568839B2 (en) 2011-01-11 2020-02-25 Capsugel Belgium Nv Hard capsules
US11319566B2 (en) 2017-04-14 2022-05-03 Capsugel Belgium Nv Process for making pullulan
US11576870B2 (en) 2017-04-14 2023-02-14 Capsugel Belgium Nv Pullulan capsules

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2897489T3 (es) 2010-06-17 2022-03-01 Hayashibara Co Método de producción de polvo que contiene pululano
JP2017505847A (ja) * 2014-02-20 2017-02-23 ダウ グローバル テクノロジーズ エルエルシー 新規の高分子量及び高均一性のエステル化セルロースエーテル

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2817264A1 (fr) * 2000-11-29 2002-05-31 Roquette Freres Pullulan alpha-1,4-isomaltohydrolase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2817264A1 (fr) * 2000-11-29 2002-05-31 Roquette Freres Pullulan alpha-1,4-isomaltohydrolase

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BARDAGE S. L. ET AL.: "Isolation of an aureobasidium pullulans polysaccharide that promotes adhesion of blastospores to water-borne paints", CAN. J. MICROBIOL., vol. 44, no. 10, 1998, pages 954 - 958, XP002977690 *
LAZARIDOU A. ET AL: "Production and characterization of pullulan from beet molasses using a nonpigmented strain of aureobasidium pullulans in batch culture", APPL. BIOCHEM. BIOTECHNOL., vol. 97, no. 1, 2002, pages 1 - 22, XP002977689 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10568839B2 (en) 2011-01-11 2020-02-25 Capsugel Belgium Nv Hard capsules
US11319566B2 (en) 2017-04-14 2022-05-03 Capsugel Belgium Nv Process for making pullulan
US11576870B2 (en) 2017-04-14 2023-02-14 Capsugel Belgium Nv Pullulan capsules
US11878079B2 (en) 2017-04-14 2024-01-23 Capsugel Belgium Nv Pullulan capsules

Also Published As

Publication number Publication date
TW200502393A (en) 2005-01-16
JP2004261132A (ja) 2004-09-24

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