WO2002085110A1 - Outil de conservation d'oeuf par congelation et outil de maintien a element cylindrique - Google Patents

Outil de conservation d'oeuf par congelation et outil de maintien a element cylindrique Download PDF

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Publication number
WO2002085110A1
WO2002085110A1 PCT/JP2002/003788 JP0203788W WO02085110A1 WO 2002085110 A1 WO2002085110 A1 WO 2002085110A1 JP 0203788 W JP0203788 W JP 0203788W WO 02085110 A1 WO02085110 A1 WO 02085110A1
Authority
WO
WIPO (PCT)
Prior art keywords
holding
egg
cylindrical member
tubular member
main body
Prior art date
Application number
PCT/JP2002/003788
Other languages
English (en)
Japanese (ja)
Inventor
Tamotsu Inoue
Futoshi Inoue
Original Assignee
Kabushikikaisha Kitazato Supply
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=18970306&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2002085110(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Kabushikikaisha Kitazato Supply filed Critical Kabushikikaisha Kitazato Supply
Publication of WO2002085110A1 publication Critical patent/WO2002085110A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/022—Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing
    • A61D19/024—Tube-like containers, e.g. straws

Definitions

  • the present invention relates to an egg cryopreservation tool used for cryopreserving eggs such as mammalian ova and embryos.
  • Cryopreservation of mammalian embryos allows the preservation of genetic resources for specific lines and varieties. It is also effective in maintaining endangered species. In addition, it is useful in treating infertility in humans.
  • cryopreservation container such as a frozen straw, frozen pial or cryotube obtained by sterilizing mammalian embryos or ova. Affix a minimal amount of vitrification liquid sufficient to enclose these embryos or eggs, seal the container for freezing and storage, and rapidly cool the container by contact with liquid nitrogen It has been proposed.
  • the cryopreservation container stored by the above method is removed from the liquid nitrogen, one end of the container is opened, and a diluent at 33 to 39 ° C is directly injected into the container. It is used for thawing and diluting the freezing of embryos or eggs. According to this method, there is an excellent effect that a mammalian embryo or ovum can be stored and thawed at a high survival rate without fear of infection by a virus or a bacterium.
  • cryopreservation such as cryo-straws, cryo-vials or cryo-tubes It was not always easy to attach the minimum amount of vitrification liquid sufficient to cover the eggs such as embryos or eggs on the inner surface of the storage container.
  • an object of the present invention is to provide an egg cryopreservation tool which can perform cryopreservation work of eggs reliably and easily. Disclosure of the invention
  • the present invention relates to a body portion formed of a cold-resistant material, a strip for attaching and holding eggs attached to one end of the body portion and formed of a flexible, transparent, and liquid nitrogen-resistant material.
  • An egg cryopreservation tool comprising a cylindrical member formed by a cold-resistant material, one end of which is detachably attached to the main body so as to be capable of enclosing an adhesion holding strip, and which is formed of a cold-resistant material.
  • the present invention also provides a holding member having a holding portion on a front end side and a holding portion on a rear end side, and a rotatable shaft between the holding portion and the holding portion of the two holding members.
  • a supported scissors forceps-like cylindrical member holding device wherein a pair of concave portions facing each other are formed in opposing holding portions of the two holding members so as to hold the cylindrical member without substantially crushing it. This is a tubular member holding device.
  • FIG. 1 is a front view of an egg cryopreservation tool according to an embodiment of the present invention.
  • FIG. 2 is a front view of the egg freezing storage tool shown in FIG. 1 in a state where a tubular member is detached from a main body member.
  • FIG. 3 is a side view of a main body member of the storage device for freezing eggs shown in FIG. Fig. 4 shows the tip of the main body of the egg cryopreservation device shown in Fig. 1 and the cylinder.
  • FIG. 4 is an enlarged front view of the shape member.
  • FIG. 5 is an enlarged cross-sectional view of a tip portion of a main body member and a tubular member of the egg cryopreservation tool shown in FIG.
  • FIG. 6 is a front view of the tubular member holding device of the present invention.
  • FIG. 7 is an enlarged view of a distal end portion of the tubular member holding device shown in FIG. 6 in a released state.
  • FIG. 8 is an explanatory diagram for explaining a holding state maintaining mechanism of the tubular member holding device of the present invention.
  • the egg cryopreservation tool 1 of the present invention comprises a main body 2 formed of a cold-resistant material, and an egg attachment holding member attached to one end of the main body 2 and formed of a flexible, transparent, liquid nitrogen-resistant material. And a tubular member 4 which is detachably attached to the main body 2 so as to enclose the strip 3 for holding eggs and is detachably attached to the main body 2 and which is formed of a cold-resistant material. It is.
  • the said main-body part is an elongated flat main-body part.
  • the egg attachment holding strip is an elongated flat film-like egg attachment holding strip.
  • the tubular member is air-permeablely sealed by a cotton-like body inserted into one end side.
  • the main body portion includes a columnar portion that holds one end of the egg attachment holding strip and that penetrates into and engages with the other end of the tubular member.
  • the other end of the cylindrical member has a tapered shape. It is preferable that the tapered portion has an enlarged diameter.
  • the egg cryopreservation tool of the present invention can be referred to as an egg vitrification storage tool, an egg or embryo cryopreservation tool, an egg or embryo vitrification storage tool.
  • the egg cryopreservation tool of the present invention comprises an egg cryopreservation tool main body member 10 and a tubular member 4 detachably attached thereto. Note that the egg cryopreservation tool main body member 10 can be used alone or as an egg cryopreservation tool without providing the tubular member 4.
  • the egg cryopreservation tool main body member 10 includes a main body 2 formed of a cold-resistant material, and an egg attachment holding formed by a flexible, transparent and liquid nitrogen-resistant material attached to one end of the main body 2. It consists of strip 3.
  • the main body 2 is an elongated flat main body.
  • the length of the main body is preferably about 50 to 200 mm.
  • the width of the flat main body is preferably about 3 to 10 mm, and the thickness is preferably about 1 to 3 mm.
  • the main body member 10 has a columnar portion 5 that holds one end of the egg attachment holding strip 3 and that penetrates into and engages with the other end of the cylindrical member 4.
  • the diameter of the columnar portion is slightly reduced in a tapered shape toward the distal end.
  • the outer diameter of the cylindrical portion is preferably about 0.5 to 3 mm.
  • a concave portion extending in the axial direction for accommodating one end of the egg attachment holding strip 3 is formed at the tip of the cylindrical portion 5.
  • the main body 2 is made of polyester (for example, polyethylene terephthalate, polybutylene terephthalate), polyolefin (for example, polyethylene, ultrahigh molecular weight polyethylene, polypropylene, ethylene-propylene copolymer, ethylene-vinyl acetate copolymer), styrene Resin (eg, Cold-resistant resins such as polystyrene, methacrylate-styrene copolymer, methacrylate-butylene-styrene copolymer, polyamide (for example, 6-nylon, 66-nylon), polysulfone, fluororesin, and polyimide are used. Those that can withstand the temperature of liquid nitrogen are preferred.
  • polyester for example, polyethylene terephthalate, polybutylene terephthalate
  • polyolefin for example, polyethylene, ultrahigh molecular weight polyethylene, polypropylene, ethylene-propylene copolymer, ethylene-vin
  • the egg attachment holding strip 3 is made of a flexible, transparent and liquid nitrogen-resistant material.
  • the strip 3 has an elongated flat film shape.
  • the strip 3 is a flexible, colorless and transparent flat film in order to facilitate the work of attaching eggs.
  • the length of the exposed portion of the egg attachment holding strip 3 is about 10 to 30 mm, the width is about 0.5 to 2.0 mm, and the thickness is about 0.01 to 0.4 mm. Is used.
  • the other end (base end) of the egg attachment holding strip 3 is housed in the recess of the main body 2 described above, and is fixed to the main body 2 with an adhesive.
  • the material for forming the egg attachment holding strip 3 include 3-fluorinated polyethylene, low-density polyethylene, medium-density polyethylene, high-density polyethylene, polycarbonate, nylon, polysulfone, polyester, polystyrene, polyimide, and ultra-high-molecular-weight polyethylene.
  • a film made of a synthetic resin such as an ethylene-vinyl acetate copolymer or a laminate thereof.
  • a liquid nitrogen-resistant material in other words, a material that does not become brittle even when in contact with liquid nitrogen is used.
  • the cylindrical member 4 is formed of a cold-resistant material, one end of which is detachably attached to the main body 2 so as to be capable of enclosing the egg attachment holding strip 3 and is detachable.
  • the tubular member 4 of this embodiment is air-permeablely sealed by a cotton-like body 12 inserted inside one end, and the other end is tapered. It is a tapered portion that expands in a shape. Then, the cotton-like body 12 housed in the tubular member 4 is fixed by the adhesive 13.
  • the cylindrical member 4 has an inner diameter and a length that can be encapsulated without contacting the egg attachment holding strip 3.
  • the tapered portion of the tubular member 4 facilitates the insertion of the strip 3 into the tubular member, and also facilitates the penetration of the cylindrical portion of the main body 2 into the tubular member.
  • Examples of the material for forming the cylindrical member 4 include 3-fluoropolyethylene, low-density polyethylene, medium-density polyethylene, high-density polyethylene, polycarbonate, nylon, polysulfone, polyester, polystyrene, polyimide, ultra-high-molecular-weight polyethylene, and ethylene.
  • Examples of the film include a film made of a synthetic resin such as a vinyl monoacetate copolymer and a laminate thereof.
  • a material resistant to liquid nitrogen in other words, a material that does not become brittle even when it comes into contact with liquid nitrogen is preferable. .
  • FIG. 6 is a front view of the tubular member holding device of the present invention.
  • FIG. 7 is a partially enlarged view of the distal end of the tubular member holding device shown in FIG. 6 in a released state.
  • the tubular member holding device 20 of the present invention is provided with holding portions 25 and 26 on the front end side and holding portions 23 and 24 on the rear end side. This is a scissors forceps-shaped tubular member holding device rotatably supported between the holding portions 25 and 26 and the grip portions 23 and 24 of the two holding members 21 and 22.
  • the opposite holding portions 25 and 26 of the two holding members 21 and 22 are provided with a pair of facing members for holding the small-diameter cylindrical member without substantially crushing it. Recesses 31 and 32 are formed.
  • the said cylindrical member holding device is for holding the said cylindrical member of the said egg cryopreservation tool. Further, it is preferable that the cylindrical member holding device includes a holding state maintaining mechanism provided on two holding members for maintaining a state of holding the cylindrical member.
  • This tubular member holding device is effectively used to hold the tubular member 4 of the above-described egg cryopreservation tool.
  • the tubular member holding device 20 of the present invention includes two holding members 21 and 22, which are substantially symmetrical.
  • the holding member 21 has a holding portion 25 on the front end side and a grip portion 23 on the rear end side.
  • the holding member 22 includes a holding portion 26 at the front end and a grip portion 24 at the rear end.
  • the holding portions 25 and 26 are provided with opposed blade portions 27 and 28 and a pair of opposed recesses 31 and 32 provided at the distal end of the holding portions 25 and 26.
  • the two holding members 21 and 22 are rotatably supported by a shaft 29 between the holding portions 25 and 26 and the holding portions 23 and 24.
  • the concave portions 31 and 32 are formed without substantially crushing the small-diameter cylindrical member. It is formed in a size that can be held. Specifically, it is formed so that the inner diameter of the circular opening formed by the concave portions 31 and 32 is slightly smaller than the outer diameter of the cylindrical member to be held.
  • the tubular member holding device includes a holding state maintaining mechanism 35, provided on two holding members 21 and 22 for maintaining a state of holding the cylindrical member. It has 36. Specifically, the two holding members 21 and 22 extend from the vicinity of the gripping portions 23 and 24 toward the other holding member. As shown in FIG. 8, a stepwise engaging portion having a sawtooth-shaped cross section is formed on the inner surface of the flat projecting portion in contact with the flat projecting portion. I have. For this reason, when the gripping portions 23, 24 of the holding members 21, 22, are pushed in the approaching direction in a state where the cylindrical member is held between the concave portions of the cylindrical member holding device 20, the holding state maintaining mechanism is provided. The saw blades 35, 36 are engaged and maintain that state.
  • the tubular member holding device 20 it is not necessary to apply a special force to maintain the tubular member in the holding state, and the tubular member 4 covers the strip 3, and the main body 2 It is easy to attach to Further, the engagement of the holding state maintaining mechanisms 35, 36 can be released by expanding the two holding members 21, 22 in the horizontal direction, in other words, in the vertical direction in FIG.
  • the tubular member holding device is made of a metal such as stainless steel or a titanium alloy.
  • the egg is collected at the tip of the pipette, the work of replacing the intracellular fluid of the egg with the equilibrium solution, and the work of replacing the extracellular fluid with the vitrification solution. Then, under a microscope, the egg was attached to the tip of the egg attachment holding strip 3 of the egg cryopreservation tool 1 of the present invention together with a small amount of vitrification liquid, and the strip portion to which the egg was attached was prepared in advance. Immerse in liquid nitrogen and freeze (vitrify). Then, the tubular member 4 was attached to the main body member 10 by using a tubular member holding device that holds the tubular member 4 prepared in advance and that state is maintained, and the tubular member holding device was removed. Then, after storing the egg cryopreservation tool that holds the vitrified eggs in the storage container (cane), store the container in a liquid nitrogen tank. Industrial applicability
  • the egg cryopreservation tool of the present invention comprises: a main body formed of a cold-resistant material; an egg attached to one end of the main body, formed of a flexible, transparent, liquid nitrogen-resistant material. It is composed of an attachment holding strip, and a tubular member which is detachably attached to the main body so as to be capable of enclosing the egg attachment holding strip and which is closed at one end and formed of a cold-resistant material. is there.
  • the provision of the egg attachment holding strip makes it easy to attach and hold eggs, such as eggs or embryos, together with the vitrification liquid, and also facilitates immersion of the eggs in liquid nitrogen.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Reproductive Health (AREA)
  • Public Health (AREA)
  • Hematology (AREA)
  • Mechanical Engineering (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un outil de conservation d'oeuf par congélation (1) qui comprend un corps principal (2) constitué d'un matériau résistant au froid, une bande de maintien d'oeuf par adhérence (3), attachée à une extrémité du corps principal (2) et formé d'un matériau souple, transparent et résistant à l'azote liquide, ainsi qu'un élément cylindrique (4) fermé à une extrémité. La bande de maintien d'oeuf par adhérence (3), constituée d'un matériau résistant au froid, est attachée au corps principal (2) de façon amovible et de manière à pouvoir être encapsulée.
PCT/JP2002/003788 2001-04-18 2002-04-16 Outil de conservation d'oeuf par congelation et outil de maintien a element cylindrique WO2002085110A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001-120225 2001-04-18
JP2001120225A JP4373025B2 (ja) 2001-04-18 2001-04-18 卵凍結保存用具および筒状部材保持器具

Publications (1)

Publication Number Publication Date
WO2002085110A1 true WO2002085110A1 (fr) 2002-10-31

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JP (1) JP4373025B2 (fr)
WO (1) WO2002085110A1 (fr)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004092358A1 (fr) * 2003-04-15 2004-10-28 Kabushiki Kaisha Kitazato Supply Outil et procede de congelation et de stockage d'oeufs
WO2006066385A1 (fr) * 2004-12-21 2006-06-29 Mcgill University Outil retractable et procede de manipulation de cellules du developpement lors de leur cryoconservation
EP2140814A1 (fr) 2008-07-03 2010-01-06 Kabushiki Kaisha Kitazato Supply Appareil pour la collecte et la cryoconservation d'un morceau de tissu
WO2011144352A1 (fr) 2010-05-21 2011-11-24 Universitat Autonoma De Barcelona Dispositif de manipulation de matériaux biologiques dans un procédé de cryoconservation et utilisation d'un tel dispositif
WO2011146998A1 (fr) 2010-05-28 2011-12-01 Sydney Ivf Limited Appareils et procédés améliorés de stockage et de micromanipulation
WO2013051522A1 (fr) 2011-10-05 2013-04-11 株式会社北里バイオファルマ Outil pour la cryoconservation des cellules vivantes
WO2013051521A1 (fr) 2011-10-04 2013-04-11 株式会社北里バイオファルマ Outil pour la cryoconservation des cellules
WO2013051520A1 (fr) 2011-10-03 2013-04-11 株式会社北里バイオファルマ Outil pour la cryoconservation des cellules vivantes
US9700038B2 (en) 2009-02-25 2017-07-11 Genea Limited Cryopreservation of biological cells and tissues
WO2019170951A1 (fr) * 2018-03-07 2019-09-12 Universidad de Córdoba Dispositif flexible pour la congélation de doses séminales
WO2020174481A1 (fr) * 2019-02-25 2020-09-03 Payeli Sravan Kumar Paille de vitrification et dispositif de cryoconservation
WO2021025017A1 (fr) 2019-08-05 2021-02-11 株式会社北里コーポレーション Plaque d'opération de prétraitement de cryoconservation de cellules
WO2023002974A1 (fr) 2021-07-21 2023-01-26 株式会社北里コーポレーション Plateau de travail pour le traitement de cellules biologiques et kit de travail pour le traitement de précongélation de cellules biologiques

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WO2011070973A1 (fr) * 2009-12-08 2011-06-16 学校法人北里研究所 Tube étroit pour la conservation par vitrification d'un embryon ou d'un ovule animal
CN102405901A (zh) * 2010-09-21 2012-04-11 北京微创介入医疗装备有限公司 一种吸取式玻璃化冷冻载体装置
CN103202285B (zh) * 2012-01-11 2015-11-25 山东艾维夫生物科技有限公司 一种细胞冷冻保存装置
CN102669088B (zh) * 2012-05-30 2013-08-14 河南省农业科学院 哺乳动物胚胎和卵母细胞玻璃化冷冻载体
DE112014002424T5 (de) 2013-05-16 2016-02-04 Mitsubishi Paper Mills Limited Hilfsmittel zur Kryokonservierung durch Vitrifikation für Zellen oder Gewebe
JP6022602B2 (ja) * 2013-10-29 2016-11-09 学校法人北里研究所 細胞または組織のガラス化凍結保存用治具
CN104642299A (zh) * 2013-11-21 2015-05-27 浙江星博生物科技有限公司 一种玻璃化冷冻载体系统
JP6124845B2 (ja) 2014-06-30 2017-05-10 三菱製紙株式会社 細胞又は組織のガラス化凍結保存用治具
WO2016063806A1 (fr) 2014-10-23 2016-04-28 三菱製紙株式会社 Outil pour la cryoconservation d'une cellule ou d'un tissu et procédé de cryoconservation
EP3228191B1 (fr) * 2014-12-02 2020-08-05 Kitazato Corporation Outil de cryoconservation de tissu biologique prélevé et procédé de congélation de fragment de tissu prélevé
WO2017099865A1 (fr) 2015-12-07 2017-06-15 Coopersurgical, Inc. Supports d'échantillon à basse température et procédés associés
JP6768955B2 (ja) 2017-06-12 2020-10-14 三菱製紙株式会社 細胞又は組織の凍結保存用治具
US11116206B2 (en) 2018-10-01 2021-09-14 Cook Medical Technologies Llc Cryocontainer
WO2020250766A1 (fr) 2019-06-12 2020-12-17 三菱製紙株式会社 Dispositif de fixation de gabarit de cryoconservation et procédé de congélation et de décongélation utilisant ledit dispositif de fixation
JP7144380B2 (ja) 2019-08-23 2022-09-29 三菱製紙株式会社 細胞又は組織の凍結保存用治具
KR102487654B1 (ko) 2020-12-02 2023-01-12 대한민국 돼지 난자의 유리화 동결 보존용 도구 및 이를 이용한 돼지 난자의 유리화 동결 보존방법
WO2023132279A1 (fr) * 2022-01-07 2023-07-13 株式会社北里コーポレーション Instrument de congélation/conservation de cellules biologiques

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WO2000021365A1 (fr) * 1998-10-14 2000-04-20 Forest Katrina T Procede de vitrification d'un specimen biologique
JP2000189155A (ja) * 1998-12-25 2000-07-11 Livestock Improvement Association Of Japan Inc 哺乳動物胚または卵子の保存方法並びに凍結胚または卵子の融解希釈方法
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JPH01277485A (ja) * 1988-04-30 1989-11-07 Shimadzu Corp 細胞凍結保存方法
JP3037141U (ja) * 1996-10-23 1997-05-06 株式会社山谷産業 プライヤー
JP3047339U (ja) * 1997-09-19 1998-04-10 株式会社スーパーツール プライヤー
JPH1190841A (ja) * 1997-09-19 1999-04-06 San Nishimura:Kk 眼鏡枠のパッド用プライヤ
WO2000021365A1 (fr) * 1998-10-14 2000-04-20 Forest Katrina T Procede de vitrification d'un specimen biologique
JP2000189155A (ja) * 1998-12-25 2000-07-11 Livestock Improvement Association Of Japan Inc 哺乳動物胚または卵子の保存方法並びに凍結胚または卵子の融解希釈方法
JP2001252293A (ja) * 2000-03-14 2001-09-18 Hyogo Prefecture 卵子または胚のガラス化用具及び方法

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7316896B2 (en) 2003-04-15 2008-01-08 Kabushiki Kaisha Kitazato Supply Egg freezing and storing tool and method
CN100368533C (zh) * 2003-04-15 2008-02-13 株式会社北里萨普利 卵冻结保存用具和卵冻结保存方法
KR100828484B1 (ko) 2003-04-15 2008-05-13 가부시키가이샤 기타자토 서플라이 알 동결보존용 도구 및 알 동결보존 방법
WO2004092358A1 (fr) * 2003-04-15 2004-10-28 Kabushiki Kaisha Kitazato Supply Outil et procede de congelation et de stockage d'oeufs
WO2006066385A1 (fr) * 2004-12-21 2006-06-29 Mcgill University Outil retractable et procede de manipulation de cellules du developpement lors de leur cryoconservation
EP2140814A1 (fr) 2008-07-03 2010-01-06 Kabushiki Kaisha Kitazato Supply Appareil pour la collecte et la cryoconservation d'un morceau de tissu
US9700038B2 (en) 2009-02-25 2017-07-11 Genea Limited Cryopreservation of biological cells and tissues
WO2011144352A1 (fr) 2010-05-21 2011-11-24 Universitat Autonoma De Barcelona Dispositif de manipulation de matériaux biologiques dans un procédé de cryoconservation et utilisation d'un tel dispositif
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US11033022B2 (en) 2010-05-28 2021-06-15 Genea Ip Holdings Pty Limited Micromanipulation and storage apparatus and methods
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WO2013051521A1 (fr) 2011-10-04 2013-04-11 株式会社北里バイオファルマ Outil pour la cryoconservation des cellules
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WO2013051522A1 (fr) 2011-10-05 2013-04-11 株式会社北里バイオファルマ Outil pour la cryoconservation des cellules vivantes
WO2019170951A1 (fr) * 2018-03-07 2019-09-12 Universidad de Córdoba Dispositif flexible pour la congélation de doses séminales
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JP2022525731A (ja) * 2019-02-25 2022-05-19 スラヴァン クマール パイェリ、 ガラス化ストロー及び凍結保存装置
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WO2023002974A1 (fr) 2021-07-21 2023-01-26 株式会社北里コーポレーション Plateau de travail pour le traitement de cellules biologiques et kit de travail pour le traitement de précongélation de cellules biologiques

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