WO2019170951A1 - Dispositif flexible pour la congélation de doses séminales - Google Patents

Dispositif flexible pour la congélation de doses séminales Download PDF

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Publication number
WO2019170951A1
WO2019170951A1 PCT/ES2019/070152 ES2019070152W WO2019170951A1 WO 2019170951 A1 WO2019170951 A1 WO 2019170951A1 ES 2019070152 W ES2019070152 W ES 2019070152W WO 2019170951 A1 WO2019170951 A1 WO 2019170951A1
Authority
WO
WIPO (PCT)
Prior art keywords
freezing
flexible
straight
doses
flexible device
Prior art date
Application number
PCT/ES2019/070152
Other languages
English (en)
Spanish (es)
Inventor
Carlos Carmelo PÉREZ MARÍN
Original Assignee
Universidad de Córdoba
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Córdoba filed Critical Universidad de Córdoba
Publication of WO2019170951A1 publication Critical patent/WO2019170951A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes

Definitions

  • the present invention consists of a device for reproductive use, and is based on a device whose body is flexible for the packaging and freezing of semen, preferably animal, and which allows to keep the body of the device completely straight when it is to be used. the content after defrosting or bending during filling and preservation.
  • the invention can be framed within reproductive technology, specifically within the field of instrumentation for artificial insemination. Its application is directed towards the veterinary sector related to sperm cryopreservation and its use in artificial insemination.
  • This invention allows the preparation of inseminating doses in a single indivisible device, commonly referred to as a straw, which guarantees that its concentration and volume is exactly what the reference laboratory considers adequate, and also that it belongs to a specific ejaculate, avoiding errors in the use of such inseminating doses. Its design offers the advantages of small-caliber containers, in which the cold transmission is optimal for the preservation of seminal material.
  • this concentration of cells is distributed in approximately 4-6 straws of a fixed volume of 0.25 or 0.5 ml each.
  • the inseminating dose should have 200-300 x 10 6 mobile sperm, which should be packed in approximately 2-4 straws for later use. And in the case of the pig, it is also mandatory for the freezing of said material to pack the inseminating dose in several straws.
  • US5868178 discloses a straw for storing a biological liquid such as animal semen, said straw comprising a thin tube open at both ends and closed by a waterproof cap coupled at one of said ends, wherein said waterproof cap is formed by a powder. that when in contact with a liquid it becomes an impermeable and leak-proof paste, and where said powder is disposed between an external block and an internal block of a fiber material that is permeable to air and liquids.
  • US2016278905 discloses a solution in which the device for storing a substance such as semen is constituted by a tube that has a gas-tight and liquid-tight cap, and in which said cap is arranged near one of its ends, and where the set has a system to control the filling of the straw; and where this device has the peculiarity that the cap comprises an indicator component that has a first predetermined color in the absence of prior contact with said substance, and a second predetermined color, of a different shade than the first color, once it has been in contact with said substance.
  • a straw comprising a tube comprising a cap placed near one of its ends, where the cap has the peculiarity that at its end a barrier pad is provided that It consists of hydrophobic threads throughout the cross-section of the tube and because after a liquid substance has come into contact with the cap, it blocks the passage of the liquid substance through it.
  • WO2017125679 discloses a device for preserving a predetermined dose of liquid-based substance, especially animal semen, consisting of a tube made of a weldable plastic material, where the tube allows a state of service in which a receiving portion intended to contain said
  • the predetermined dose has a circular cross-section, while a sealing portion comprises a welded step or recess and a transition zone.
  • a tube-shaped container which comprises a cap that is gas permeable and liquid impermeable, and which is arranged in the tube near one end, and where It has an indicator configured to emit, at least when the straw is full, a peak of predetermined wavelength.
  • patent WO2016170263 stands out where another type of straw-shaped device is disclosed that has a gas-permeable but liquid-impermeable cap, where the tube is made of a thermochromic material that changes color to a preset temperature threshold, so that the tube has a first visual appearance if the temperature thereof is below said preset threshold and a second aspect that is different from the first visual aspect if the temperature thereof is above said threshold preset
  • the known solutions refer to other aspects related to the straws, especially the different closures designed for the ends, which gives them particularities in their use; to elements of signaling or emission of information of the liquid, or semen, that lodge in its interior; or to the need to use several of these devices for the reproductive purpose.
  • a device for reproductive use is described, and consists of a device whose body is flexible and serves for the packaging and freezing of semen, preferably animal.
  • the semen of certain animal species such as the horse, the dog or the pig, is frozen in ducts generally known as 0.5 or 0.25 ml straws, and each inseminating dose is usually composed of 4-6 of these straws .
  • insemination catheters When carrying out artificial insemination, various types of insemination catheters are available which, by means of a guide that is introduced into the straw, expel the contents within the female's genital apparatus and then is removed, repeating this action as many times as straws should be used.
  • the 4-6 sperm doses are deposited in a container and then loaded into a syringe for later introduction into the female's genital apparatus (approx. 1.00-1.25 ml).
  • dose manipulation is complicated and keeps the uterus in communication with the outside for the duration of the preparation of the various straws to be used.
  • the seminal manipulation of such a small volume can detrimentally affect its quality and part of it may be lost during this process.
  • straws of 0.25 ml are usually used and inseminating doses should contain around 250 and 500 million sperm.
  • frozen semen it is recommended to inseminate mares every 6-8 hours and as close as possible to ovulation to achieve maximum fertility.
  • straws of 0.25 and 0.5 ml are also used, it being recommended that these straws have between 50 and 150 million sperm cells, and it is necessary to inseminate with approx. 200-300 million.
  • 5-6 straws are used to reach a total concentration of 1000 million sperm per inseminating dose.
  • the new type of device defined in the present invention reduces the handling difficulties encountered during the use of the existing devices in the state of the art with several straws, offering an easy to defrost and manipulate presentation, and that guarantees maximum Hygiene and quality of the sample.
  • each ejaculate corresponding to a dog, a horse or a pig is usually frozen in 2-5 straws, as mentioned above.
  • the doses of the same species or of the same animal are not always similar, and the quality of the ejaculate, its volume and concentration will determine the number of straws that make up the inseminant dose. This means that when selling seminal doses or using them, there may be some doubt about their contents, especially when the doses have been able to travel to different storage tanks.
  • the device object of the present invention guarantees that the seminal dose housed in its Inside is exactly the one that has been prepared in the laboratory from a certain ejaculate, with a fixed concentration and volume, preventing it from suffering variations during the processing and transport of conventional devices, packages or straws.
  • the flexible device consists of a conduit of a preferably plastic nature between 1 and 5 mm. in diameter for semen packaging that has a plurality of straight bodies, joined through an area that comprises a flexible and flexible wall, in the form of a bellows or accordion, which allows the device to be left completely straight when the content is to be used after the defrosting, or zigzagging or flexed during filling and preservation.
  • this device for semen packaging comprises between 2 and 7 straight parts, between 10 and 20 cm in length, joined by the aforementioned folding areas, which allows to decide what total volume will be used in freezing, since it gives the possibility of cutting that area that you do not want to use, maintaining the characteristic of making the inseminant dose indivisible.
  • closure of the device is achieved by sealing at least one of its ends with double cotton and polyvinyl pyrrolidone to ensure its closure upon contact with the liquid, being normally prior to use or after its use. Open use at both ends.
  • the device incorporates a stiffener or a slight plastic connection between the different bodies or straight areas that compose it to prevent said container from losing its shape during the process of handling, filling or defrosting.
  • Figure 1 Scheme of a flexible device for freezing sperm made in accordance with the object of the present invention, when said devices are folded or in a zigzag shape.
  • Figure 2 Expanded detail of the plastic joint between the different bodies that make up the flexible device, and the flexion system used to connect the different bodies of the device and which allows it to be kept in a folded position or can be extended for use direct.
  • Figure 3 Scheme of a flexible device for freezing sperm made in accordance with the object of the present invention when said device is straight.
  • the flexible device for freezing seminal doses can be seen to consist of a conduit of a plastic nature comprising a plurality of straight bodies (1) joined at their ends by joints (2) with folding and flexible walls in bellows shape that are precisely those that allow the device to have a folded (Fig. 1) or straight (Fig. 3) configuration, and that is closed by using a plug (3) at least at one of its ends.
  • a preferred embodiment of the invention consists of a conduit with 5 straight bodies (1), said straight bodies having a diameter of 2 mm, and a length of 13 cm; wherein the plug (3) is constituted by a double cotton and polyvinylpyrrolidone and is disposed about 2 cm from the end of the duct; and where, in order to maintain the folded shape between the different straight bodies (1), there is a plurality of stiffeners (4) or light plastic connections that fix said straight bodies (1).

Abstract

La présente invention concerne un dispositif qui destiné à un usage de reproduction et repose sur un dispositif dont le corps est flexible pour le conditionnement et la congélation de sperme, de préférence animal et qui permet de maintenir le corps du dispositif totalement droit lorsqu'on va utiliser le contenu après sa décongélation ou en flexion pendant son remplissage et sa conservation.
PCT/ES2019/070152 2018-03-07 2019-03-08 Dispositif flexible pour la congélation de doses séminales WO2019170951A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES201830304U ES1208736Y (es) 2018-03-07 2018-03-07 Dispositivo flexible para la congelacion de dosis seminales
ESU201830304 2018-03-07

Publications (1)

Publication Number Publication Date
WO2019170951A1 true WO2019170951A1 (fr) 2019-09-12

Family

ID=61696150

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2019/070152 WO2019170951A1 (fr) 2018-03-07 2019-03-08 Dispositif flexible pour la congélation de doses séminales

Country Status (2)

Country Link
ES (1) ES1208736Y (fr)
WO (1) WO2019170951A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5916144A (en) * 1995-05-09 1999-06-29 The Curators Of The University Of Missouri System for introducing a fluid into the uterus of an animal
GB2342912A (en) * 1998-05-20 2000-04-26 Imv Technologies Bag for packaging liquid substances for animal artificial insemination
EP1066802A2 (fr) * 1999-07-09 2001-01-10 Livestock Improvement Association of Japan Inc. Dispositif et méthode pour l'injection de semence ou d'embryons dans un animal domestique
US20020151765A1 (en) * 2000-01-03 2002-10-17 Javier Gil Pascual Artificial insemination device for pigs
WO2002085110A1 (fr) * 2001-04-18 2002-10-31 Kabushikikaisha Kitazato Supply Outil de conservation d'oeuf par congelation et outil de maintien a element cylindrique
US20150282916A1 (en) * 2013-07-03 2015-10-08 Brad K. Stroud Method, apparatus and kit for artificial insemination of bovine

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5916144A (en) * 1995-05-09 1999-06-29 The Curators Of The University Of Missouri System for introducing a fluid into the uterus of an animal
GB2342912A (en) * 1998-05-20 2000-04-26 Imv Technologies Bag for packaging liquid substances for animal artificial insemination
EP1066802A2 (fr) * 1999-07-09 2001-01-10 Livestock Improvement Association of Japan Inc. Dispositif et méthode pour l'injection de semence ou d'embryons dans un animal domestique
US20020151765A1 (en) * 2000-01-03 2002-10-17 Javier Gil Pascual Artificial insemination device for pigs
WO2002085110A1 (fr) * 2001-04-18 2002-10-31 Kabushikikaisha Kitazato Supply Outil de conservation d'oeuf par congelation et outil de maintien a element cylindrique
US20150282916A1 (en) * 2013-07-03 2015-10-08 Brad K. Stroud Method, apparatus and kit for artificial insemination of bovine

Also Published As

Publication number Publication date
ES1208736Y (es) 2018-06-21
ES1208736U (es) 2018-03-28

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