WO2019170951A1 - Flexible device for freezing semen doses - Google Patents

Flexible device for freezing semen doses Download PDF

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Publication number
WO2019170951A1
WO2019170951A1 PCT/ES2019/070152 ES2019070152W WO2019170951A1 WO 2019170951 A1 WO2019170951 A1 WO 2019170951A1 ES 2019070152 W ES2019070152 W ES 2019070152W WO 2019170951 A1 WO2019170951 A1 WO 2019170951A1
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WO
WIPO (PCT)
Prior art keywords
freezing
flexible
straight
doses
flexible device
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PCT/ES2019/070152
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Spanish (es)
French (fr)
Inventor
Carlos Carmelo PÉREZ MARÍN
Original Assignee
Universidad de Córdoba
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Publication of WO2019170951A1 publication Critical patent/WO2019170951A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes

Definitions

  • the present invention consists of a device for reproductive use, and is based on a device whose body is flexible for the packaging and freezing of semen, preferably animal, and which allows to keep the body of the device completely straight when it is to be used. the content after defrosting or bending during filling and preservation.
  • the invention can be framed within reproductive technology, specifically within the field of instrumentation for artificial insemination. Its application is directed towards the veterinary sector related to sperm cryopreservation and its use in artificial insemination.
  • This invention allows the preparation of inseminating doses in a single indivisible device, commonly referred to as a straw, which guarantees that its concentration and volume is exactly what the reference laboratory considers adequate, and also that it belongs to a specific ejaculate, avoiding errors in the use of such inseminating doses. Its design offers the advantages of small-caliber containers, in which the cold transmission is optimal for the preservation of seminal material.
  • this concentration of cells is distributed in approximately 4-6 straws of a fixed volume of 0.25 or 0.5 ml each.
  • the inseminating dose should have 200-300 x 10 6 mobile sperm, which should be packed in approximately 2-4 straws for later use. And in the case of the pig, it is also mandatory for the freezing of said material to pack the inseminating dose in several straws.
  • US5868178 discloses a straw for storing a biological liquid such as animal semen, said straw comprising a thin tube open at both ends and closed by a waterproof cap coupled at one of said ends, wherein said waterproof cap is formed by a powder. that when in contact with a liquid it becomes an impermeable and leak-proof paste, and where said powder is disposed between an external block and an internal block of a fiber material that is permeable to air and liquids.
  • US2016278905 discloses a solution in which the device for storing a substance such as semen is constituted by a tube that has a gas-tight and liquid-tight cap, and in which said cap is arranged near one of its ends, and where the set has a system to control the filling of the straw; and where this device has the peculiarity that the cap comprises an indicator component that has a first predetermined color in the absence of prior contact with said substance, and a second predetermined color, of a different shade than the first color, once it has been in contact with said substance.
  • a straw comprising a tube comprising a cap placed near one of its ends, where the cap has the peculiarity that at its end a barrier pad is provided that It consists of hydrophobic threads throughout the cross-section of the tube and because after a liquid substance has come into contact with the cap, it blocks the passage of the liquid substance through it.
  • WO2017125679 discloses a device for preserving a predetermined dose of liquid-based substance, especially animal semen, consisting of a tube made of a weldable plastic material, where the tube allows a state of service in which a receiving portion intended to contain said
  • the predetermined dose has a circular cross-section, while a sealing portion comprises a welded step or recess and a transition zone.
  • a tube-shaped container which comprises a cap that is gas permeable and liquid impermeable, and which is arranged in the tube near one end, and where It has an indicator configured to emit, at least when the straw is full, a peak of predetermined wavelength.
  • patent WO2016170263 stands out where another type of straw-shaped device is disclosed that has a gas-permeable but liquid-impermeable cap, where the tube is made of a thermochromic material that changes color to a preset temperature threshold, so that the tube has a first visual appearance if the temperature thereof is below said preset threshold and a second aspect that is different from the first visual aspect if the temperature thereof is above said threshold preset
  • the known solutions refer to other aspects related to the straws, especially the different closures designed for the ends, which gives them particularities in their use; to elements of signaling or emission of information of the liquid, or semen, that lodge in its interior; or to the need to use several of these devices for the reproductive purpose.
  • a device for reproductive use is described, and consists of a device whose body is flexible and serves for the packaging and freezing of semen, preferably animal.
  • the semen of certain animal species such as the horse, the dog or the pig, is frozen in ducts generally known as 0.5 or 0.25 ml straws, and each inseminating dose is usually composed of 4-6 of these straws .
  • insemination catheters When carrying out artificial insemination, various types of insemination catheters are available which, by means of a guide that is introduced into the straw, expel the contents within the female's genital apparatus and then is removed, repeating this action as many times as straws should be used.
  • the 4-6 sperm doses are deposited in a container and then loaded into a syringe for later introduction into the female's genital apparatus (approx. 1.00-1.25 ml).
  • dose manipulation is complicated and keeps the uterus in communication with the outside for the duration of the preparation of the various straws to be used.
  • the seminal manipulation of such a small volume can detrimentally affect its quality and part of it may be lost during this process.
  • straws of 0.25 ml are usually used and inseminating doses should contain around 250 and 500 million sperm.
  • frozen semen it is recommended to inseminate mares every 6-8 hours and as close as possible to ovulation to achieve maximum fertility.
  • straws of 0.25 and 0.5 ml are also used, it being recommended that these straws have between 50 and 150 million sperm cells, and it is necessary to inseminate with approx. 200-300 million.
  • 5-6 straws are used to reach a total concentration of 1000 million sperm per inseminating dose.
  • the new type of device defined in the present invention reduces the handling difficulties encountered during the use of the existing devices in the state of the art with several straws, offering an easy to defrost and manipulate presentation, and that guarantees maximum Hygiene and quality of the sample.
  • each ejaculate corresponding to a dog, a horse or a pig is usually frozen in 2-5 straws, as mentioned above.
  • the doses of the same species or of the same animal are not always similar, and the quality of the ejaculate, its volume and concentration will determine the number of straws that make up the inseminant dose. This means that when selling seminal doses or using them, there may be some doubt about their contents, especially when the doses have been able to travel to different storage tanks.
  • the device object of the present invention guarantees that the seminal dose housed in its Inside is exactly the one that has been prepared in the laboratory from a certain ejaculate, with a fixed concentration and volume, preventing it from suffering variations during the processing and transport of conventional devices, packages or straws.
  • the flexible device consists of a conduit of a preferably plastic nature between 1 and 5 mm. in diameter for semen packaging that has a plurality of straight bodies, joined through an area that comprises a flexible and flexible wall, in the form of a bellows or accordion, which allows the device to be left completely straight when the content is to be used after the defrosting, or zigzagging or flexed during filling and preservation.
  • this device for semen packaging comprises between 2 and 7 straight parts, between 10 and 20 cm in length, joined by the aforementioned folding areas, which allows to decide what total volume will be used in freezing, since it gives the possibility of cutting that area that you do not want to use, maintaining the characteristic of making the inseminant dose indivisible.
  • closure of the device is achieved by sealing at least one of its ends with double cotton and polyvinyl pyrrolidone to ensure its closure upon contact with the liquid, being normally prior to use or after its use. Open use at both ends.
  • the device incorporates a stiffener or a slight plastic connection between the different bodies or straight areas that compose it to prevent said container from losing its shape during the process of handling, filling or defrosting.
  • Figure 1 Scheme of a flexible device for freezing sperm made in accordance with the object of the present invention, when said devices are folded or in a zigzag shape.
  • Figure 2 Expanded detail of the plastic joint between the different bodies that make up the flexible device, and the flexion system used to connect the different bodies of the device and which allows it to be kept in a folded position or can be extended for use direct.
  • Figure 3 Scheme of a flexible device for freezing sperm made in accordance with the object of the present invention when said device is straight.
  • the flexible device for freezing seminal doses can be seen to consist of a conduit of a plastic nature comprising a plurality of straight bodies (1) joined at their ends by joints (2) with folding and flexible walls in bellows shape that are precisely those that allow the device to have a folded (Fig. 1) or straight (Fig. 3) configuration, and that is closed by using a plug (3) at least at one of its ends.
  • a preferred embodiment of the invention consists of a conduit with 5 straight bodies (1), said straight bodies having a diameter of 2 mm, and a length of 13 cm; wherein the plug (3) is constituted by a double cotton and polyvinylpyrrolidone and is disposed about 2 cm from the end of the duct; and where, in order to maintain the folded shape between the different straight bodies (1), there is a plurality of stiffeners (4) or light plastic connections that fix said straight bodies (1).

Abstract

The present invention relates to a device intended for reproductive use, which is based on a device having a flexible body for packaging and freezing semen, preferably animal semen, and which allows the body of the device to be completely straight when the content thereof is to be used after thawing, or allows the body to be bent during filling and preservation.

Description

DESCRIPCIÓN  DESCRIPTION
DISPOSITIVO FLEXIBLE PARA LA CONGELACIÓN DE DOSIS SEMINALES FLEXIBLE DEVICE FOR FREEZING WEEKLY DOSE
Campo de la invención Field of the Invention
La presente invención consiste en un dispositivo destinado a un uso reproductivo, y se basa en un dispositivo cuyo cuerpo es flexible para el envasado y congelación de semen, preferentemente animal, y que permite mantener el cuerpo del dispositivo recto por completo cuando se va a utilizar el contenido tras su descongelación o flexionada durante su llenado y conservación. The present invention consists of a device for reproductive use, and is based on a device whose body is flexible for the packaging and freezing of semen, preferably animal, and which allows to keep the body of the device completely straight when it is to be used. the content after defrosting or bending during filling and preservation.
El invento puede encuadrarse dentro de la tecnología reproductiva, concretamente dentro del campo de la instrumentación para inseminación artificial. Su aplicación está dirigida hacia el sector veterinario relacionado con la criopreservación espermática y su utilización en la inseminación artificial. Este invento permite la preparación de dosis inseminantes en un único dispositivo indivisible, comúnmente denominado como pajuela, que garantiza que su concentración y volumen sea exactamente la que el laboratorio de referencia estima como adecuada, y además que pertenezca a un eyaculado concreto, evitando errores en el uso de tales dosis inseminantes. Su diseño ofrece las ventajas propias de envases de pequeño calibre, en las cuales la transmisión de frío es óptima para la preservación del material seminal. The invention can be framed within reproductive technology, specifically within the field of instrumentation for artificial insemination. Its application is directed towards the veterinary sector related to sperm cryopreservation and its use in artificial insemination. This invention allows the preparation of inseminating doses in a single indivisible device, commonly referred to as a straw, which guarantees that its concentration and volume is exactly what the reference laboratory considers adequate, and also that it belongs to a specific ejaculate, avoiding errors in the use of such inseminating doses. Its design offers the advantages of small-caliber containers, in which the cold transmission is optimal for the preservation of seminal material.
Estado de la técnica State of the art
Es conocido dentro del sector de la tecnología reproductiva que una práctica común y cada vez más demandada en Reproducción Animal es la criopreservación de material seminal en las diferentes especies animales con diferentes objetivos, entre los que se encuentra la conservación de material genético de animales valiosos por su funcionalidad o por encontrarse en peligro de extinción o amenazados. Así mismo, la criopreservación espermática facilita la difusión del material genético y su conservación indefinida, y constituye una herramienta indispensable a la hora de optimizar el manejo de sementales o reducir los riesgos sanitarios asociados a la monta natural. En el caso de la congelación de semen de caballo, las muestras son diluidas con determinadas sustancias crioprotectoras y se preparan de manera que cada dosis inseminante alcance un total de 250-500 x 106 espermatozoides móviles. Para ello, esta concentración de células es repartida en aproximadamente 4-6 pajuelas de un volumen fijo de 0.25 o 0.5 mi cada una. Si nos referimos a semen canino congelado, la dosis inseminante debe tener 200-300 x 106 espermatozoides móviles, los cuales se deben envasar en aproximadamente 2-4 pajuelas para su posterior uso. Y en el caso del porcino, también es preceptivo para la congelación de dicho material envasar la dosis inseminante en varias pajuelas. It is known within the reproductive technology sector that a common and increasingly demanded practice in Animal Reproduction is the cryopreservation of seminal material in different animal species with different objectives, among which is the conservation of genetic material of valuable animals by its functionality or because it is in danger of extinction or threatened. Likewise, sperm cryopreservation facilitates the diffusion of genetic material and its indefinite conservation, and constitutes an indispensable tool when it comes to optimizing the management of stallions or reducing the health risks associated with the natural mountain. In the case of freezing horse semen, the samples are diluted with certain cryoprotective substances and are prepared so that each inseminating dose reaches a total of 250-500 x 10 6 mobile sperm. For this, this concentration of cells is distributed in approximately 4-6 straws of a fixed volume of 0.25 or 0.5 ml each. If we refer to frozen canine semen, the inseminating dose should have 200-300 x 10 6 mobile sperm, which should be packed in approximately 2-4 straws for later use. And in the case of the pig, it is also mandatory for the freezing of said material to pack the inseminating dose in several straws.
En el mercado existen pajuelas de plástico, resina ionómera o de otros materiales, con un volumen de 0.25 y 0.5 mi. Numerosos estudios científicos han descrito cómo se produce la transmisión de calor en dichos envases durante los procesos de congelación con la intención de obtener unas rampas de descenso de temperatura óptimas para la supervivencia espermática tras la descongelación. In the market there are plastic straws, ionomer resin or other materials, with a volume of 0.25 and 0.5 ml. Numerous scientific studies have described how heat transmission occurs in these containers during freezing processes with the intention of obtaining optimum temperature drop ramps for sperm survival after thawing.
En este sentido, a continuación se detalla el contenido de ciertos documentos relacionados con el campo de aplicación de la invención, en los cuales se hace referencia al diseño de dispositivos convencionales utilizados para congelar semen. In this regard, the contents of certain documents related to the field of application of the invention are detailed below, in which reference is made to the design of conventional devices used to freeze semen.
La patente US5868178 divulga una pajita para almacenar un líquido biológico tal como semen animal, comprendiendo dicha pajita un tubo fino abierto en ambos extremos y cerrado por un tapón impermeable acoplado en uno de dichos extremos, en el que dicho tapón impermeable está formado por un polvo que al ponerse en contacto con un líquido se transforma en una pasta impermeable y a prueba de fugas, y donde dicho polvo se dispone entre un taco externo y un taco interno de un material de fibra que es permeable al aire y a líquidos. US5868178 discloses a straw for storing a biological liquid such as animal semen, said straw comprising a thin tube open at both ends and closed by a waterproof cap coupled at one of said ends, wherein said waterproof cap is formed by a powder. that when in contact with a liquid it becomes an impermeable and leak-proof paste, and where said powder is disposed between an external block and an internal block of a fiber material that is permeable to air and liquids.
La patente US2016278905 divulga una solución en la que el dispositivo destinado a almacenar una sustancia como semen está constituido por un tubo que dispone de un tapón hermético a los gases y estanco a los líquidos, y en el que dicho tapón está dispuesto cerca de uno de sus extremos, y donde el conjunto tiene un sistema para controlar el llenado de la pajita; y donde este dispositivo tiene la particularidad de que el tapón comprende un componente indicador que tiene un primer color predeterminado en ausencia de contacto previo con dicha sustancia, y un segundo color predeterminado, de un tono diferente al del primer color, una vez que ha estado en contacto con dicha sustancia. También se conoce lo divulgado en la patente US2017065390 donde se da a conocer una pajita que comprende un tubo que comprende un tapón colocado cerca de uno de sus extremos, donde el tapón tiene la particularidad de que en su extremo se dispone una almohadilla de barrera que consiste en hilos hidrófobos en toda la sección transversal del tubo y porque después de que una sustancia líquida haya entrado en contacto con el tapón, este bloquea el paso de la sustancia líquida a través del mismo. US2016278905 discloses a solution in which the device for storing a substance such as semen is constituted by a tube that has a gas-tight and liquid-tight cap, and in which said cap is arranged near one of its ends, and where the set has a system to control the filling of the straw; and where this device has the peculiarity that the cap comprises an indicator component that has a first predetermined color in the absence of prior contact with said substance, and a second predetermined color, of a different shade than the first color, once it has been in contact with said substance. It is also known what is disclosed in the patent US2017065390 where a straw is disclosed comprising a tube comprising a cap placed near one of its ends, where the cap has the peculiarity that at its end a barrier pad is provided that It consists of hydrophobic threads throughout the cross-section of the tube and because after a liquid substance has come into contact with the cap, it blocks the passage of the liquid substance through it.
La patente WO2017125679 divulga un dispositivo para conservar una dosis predeterminada de sustancia basada en líquido, especialmente semen animal, constituido por un tubo hecho de un material plástico soldable, donde el tubo permite un estado de servicio en el que una porción receptora destinada a contener dicha dosis predeterminada tiene una sección transversal circular, mientras que una porción de sellado comprende un escalón o rebaje soldado y una zona de transición. WO2017125679 discloses a device for preserving a predetermined dose of liquid-based substance, especially animal semen, consisting of a tube made of a weldable plastic material, where the tube allows a state of service in which a receiving portion intended to contain said The predetermined dose has a circular cross-section, while a sealing portion comprises a welded step or recess and a transition zone.
Es también conocido lo divulgado en la patente CN 105873541 donde se describe un recipiente en forma de tubo que comprende un tapón que es permeable a los gases e impermeable a los líquidos, y que está dispuesto en el tubo cerca de un extremo, y donde se dispone de un indicador configurado para emitir, al menos cuando la pajita del está llena, un pico de longitud de onda predeterminada. It is also known what is disclosed in the patent CN 105873541 where a tube-shaped container is described which comprises a cap that is gas permeable and liquid impermeable, and which is arranged in the tube near one end, and where It has an indicator configured to emit, at least when the straw is full, a peak of predetermined wavelength.
Se destaca, a su vez, la patente WO2016170263 donde se divulga otro tipo de dispositivo en forma de pajita que dispone de un tapón permeable a los gases pero impermeable a los líquidos, donde el tubo está hecho de un material termocrómico que cambia de color a un umbral de temperatura preestablecido, de modo que el tubo tiene una primera apariencia visual si la temperatura del mismo está por debajo de dicho umbral preestablecido y un segundo aspecto que es diferente del primer aspecto visual si la temperatura del mismo está por encima de dicho umbral preestablecido. In turn, patent WO2016170263 stands out where another type of straw-shaped device is disclosed that has a gas-permeable but liquid-impermeable cap, where the tube is made of a thermochromic material that changes color to a preset temperature threshold, so that the tube has a first visual appearance if the temperature thereof is below said preset threshold and a second aspect that is different from the first visual aspect if the temperature thereof is above said threshold preset
Finalmente, se cita que los dispositivos convencionales tienen una terminación de tipo de tres partes, originalmente descrita en la patente FR995878, y que tiene en el extremo dos piezas de una sustancia fibrosa que rodean a un polvo, el cual en contacto con un líquido queda solidificado y sella el extremo del tubo o pajuela. Diversas patentes han sido desarrolladas también con ciertas modificaciones para alcanzar un método de sellado de pajuelas más eficaz donde se desarrollan sistemas que reducen la pérdida de material biológico durante su envasado en la pajuela por alojarse en la pieza de sellado, es decir, en el material de fibra y polvo que hay en el extremo. Finally, it is cited that conventional devices have a three-part type termination, originally described in patent FR995878, and that it has at the end two pieces of a fibrous substance surrounding a powder, which in contact with a liquid remains solidified and seals the end of the tube or straw. Various patents have also been developed with certain modifications to achieve a more efficient method of sealing straws where systems are developed that reduce the loss of biological material during packaging in the straw by staying in the sealing piece, that is, in the material of fiber and dust in the end.
Teniendo en cuenta las particularidades de los dispositivos conocidos dentro de este sector industrial, se puede advertir que las soluciones conocidas se refieren a otros aspectos relacionados con las pajuelas, en especial a los diferentes cierres diseñados para los extremos, lo que les confiere particularidades en su uso; a elementos de señalización o emisión de información del líquido, o semen, que albergan en su interior; o a la necesidad de utilizar varios de estos dispositivos para la finalidad reproductiva. Taking into account the particularities of the known devices within this industrial sector, it can be noted that the known solutions refer to other aspects related to the straws, especially the different closures designed for the ends, which gives them particularities in their use; to elements of signaling or emission of information of the liquid, or semen, that lodge in its interior; or to the need to use several of these devices for the reproductive purpose.
Habida cuenta de las soluciones y antecedentes existentes en el estado de la técnica, se señala que ninguna de estas invenciones tiene como objetivo el garantizar el envasado de una dosis completa de semen que además mantenga las características propias de congelación que ofrecen las pajuelas cilindricas convencionales. Por tanto, por medio de la presente invención, se resuelve el problema técnico de que, a diferencia de las existentes, el envase o pajuela se desdoble para ser usada para inseminación, sin tener que vaciar previamente su contenido en otro recipiente, y utilizándose un único dispositivo. Given the solutions and background in the state of the art, it is pointed out that none of these inventions aims to guarantee the packaging of a full dose of semen that also maintains the characteristics of freezing offered by conventional cylindrical straws. Therefore, by means of the present invention, the technical problem is solved that, unlike the existing ones, the container or straw is folded to be used for insemination, without having to previously empty its contents into another container, and using a single device
Descripción de la invención Description of the invention
En la presente invención se describe un dispositivo destinado a un uso reproductivo, y consiste en un dispositivo cuyo cuerpo es flexible y sirve para el envasado y congelación de semen, preferentemente animal. In the present invention a device for reproductive use is described, and consists of a device whose body is flexible and serves for the packaging and freezing of semen, preferably animal.
En la práctica rutinaria, el semen de ciertas especies animales como el caballo, el perro o el cerdo, se congela en conductos generalmente conocidos como pajuelas de 0.5 o 0.25 mi, y cada dosis inseminante suele estar compuesta por entre 4-6 de dichas pajuelas. In routine practice, the semen of certain animal species such as the horse, the dog or the pig, is frozen in ducts generally known as 0.5 or 0.25 ml straws, and each inseminating dose is usually composed of 4-6 of these straws .
A la hora de llevar a cabo la inseminación artificial se encuentran disponibles diversos tipos de catéteres de inseminación los que, mediante una guía que se introduce en la pajuela, expelen el contenido dentro del aparato genital de la hembra y luego es retirada, repitiendo esta acción tantas veces como pajuelas deban ser utilizadas. En otras ocasiones, las 4-6 dosis espermáticas son depositadas en un recipiente y seguidamente cargadas en una jeringa para su posterior introducción en el aparato genital de la hembra (aprox. 1.00-1.25 mi). En el primer caso, la manipulación de la dosis resulta complicada y mantiene el útero en comunicación con el exterior durante el tiempo que dura la preparación de las diversas pajuelas que van a utilizarse. En el segundo caso, la manipulación seminal de un volumen tan pequeño puede afectar de manera detrimental a su calidad y puede perderse parte de éste durante este proceso. When carrying out artificial insemination, various types of insemination catheters are available which, by means of a guide that is introduced into the straw, expel the contents within the female's genital apparatus and then is removed, repeating this action as many times as straws should be used. On other occasions, the 4-6 sperm doses are deposited in a container and then loaded into a syringe for later introduction into the female's genital apparatus (approx. 1.00-1.25 ml). In the first case, dose manipulation is complicated and keeps the uterus in communication with the outside for the duration of the preparation of the various straws to be used. In the second case, the seminal manipulation of such a small volume can detrimentally affect its quality and part of it may be lost during this process.
Si nos fijamos en las actuales recomendaciones hechas para la gestión del semen congelado equino, se suelen utilizar pajuelas de 0.25 mi y las dosis inseminantes deben albergar en torno a 250 y 500 millones de espermatozoides. En el caso de semen congelado se recomienda inseminar a las yeguas cada 6-8 horas y lo más próximo posible a la ovulación para alcanzar la máxima fertilidad. En el caso del perro también se utilizan pajuelas de 0.25 y 0.5 mi, recomendándose que dichas pajuelas tengan entre 50 y 150 millones de espermatozoides, y siendo necesario inseminar con aprox. 200-300 millones. En cerdo se utilizan 5-6 pajuelas para alcanzar una concentración total de 1000 millones de espermatozoides por dosis inseminante. If we look at the current recommendations made for the management of equine frozen semen, straws of 0.25 ml are usually used and inseminating doses should contain around 250 and 500 million sperm. In the case of frozen semen it is recommended to inseminate mares every 6-8 hours and as close as possible to ovulation to achieve maximum fertility. In the case of the dog, straws of 0.25 and 0.5 ml are also used, it being recommended that these straws have between 50 and 150 million sperm cells, and it is necessary to inseminate with approx. 200-300 million. In pigs, 5-6 straws are used to reach a total concentration of 1000 million sperm per inseminating dose.
El nuevo tipo de dispositivo que se define en la presente invención reduce las dificultades de manejo encontradas durante los usos de los dispositivos existentes en el estado de la técnica con varias pajuelas, ofreciendo una presentación fácil de descongelar y manipular, y que garantiza al máximo la higiene y calidad de la muestra. The new type of device defined in the present invention reduces the handling difficulties encountered during the use of the existing devices in the state of the art with several straws, offering an easy to defrost and manipulate presentation, and that guarantees maximum Hygiene and quality of the sample.
Cuando se analiza la implicación práctica de este invento se aprecia que puede mejorar el proceso de producción de dosis seminales, así como el éxito comercial de la inseminación con semen congelado, haciendo el proceso más práctico, eficiente y controlado. Con todo ello se pretende simplificar el proceso y reducir los errores humanos asociados a la manipulación de material criopreservado. When the practical implication of this invention is analyzed, it is appreciated that it can improve the production process of seminal doses, as well as the commercial success of insemination with frozen semen, making the process more practical, efficient and controlled. With all this, it is intended to simplify the process and reduce the human errors associated with the manipulation of cryopreserved material.
En la actualidad, cada eyaculado correspondiente a un perro, a un caballo o a un cerdo suele congelarse en 2-5 pajuelas, como se mencionó anteriormente. No siempre las dosis de una misma especie o de un mismo animal son similares, y la calidad del eyaculado, su volumen y su concentración determinará el número de pajuelas que conforman la dosis inseminante. Esto hace que a la hora de vender dosis seminales o de utilizarlas pueda existir alguna duda sobre su contenido, más aún cuando las dosis han podido viajar a diferentes tanques de almacenamiento. Currently, each ejaculate corresponding to a dog, a horse or a pig is usually frozen in 2-5 straws, as mentioned above. The doses of the same species or of the same animal are not always similar, and the quality of the ejaculate, its volume and concentration will determine the number of straws that make up the inseminant dose. This means that when selling seminal doses or using them, there may be some doubt about their contents, especially when the doses have been able to travel to different storage tanks.
El dispositivo objeto de la presente invención garantiza que la dosis seminal alojada en su interior es exactamente la que se ha preparado en el laboratorio a partir de un determinado eyaculado, con una concentración y volumen fijos, evitando que pueda sufrir variaciones durante el procesado y transporte de los dispositivos, envases o pajuelas convencionales. The device object of the present invention guarantees that the seminal dose housed in its Inside is exactly the one that has been prepared in the laboratory from a certain ejaculate, with a fixed concentration and volume, preventing it from suffering variations during the processing and transport of conventional devices, packages or straws.
En ocasiones, los usuarios no se siguen las especificaciones hechas por el laboratorio que prepara las dosis inseminantes y, en lugar de utilizar el número de dispositivos recomendados, algunos técnicos la reducen a la mitad con la intención de tener más oportunidades para inseminar a uno o varios animales, comprometiendo la fertilidad esperada. Este nuevo dispositivo envasador permite al consumidor tener la certeza de que va a utilizar la dosis inseminante tal y como la ha preparado el Centro Homologado de Inseminación, lo que podría incrementar los resultados de fertilidad al cumplirse con rigor las recomendaciones de las dosis inseminantes. Sometimes, users do not follow the specifications made by the laboratory that prepares the inseminating doses and, instead of using the number of recommended devices, some technicians reduce it by half with the intention of having more opportunities to inseminate one or more. several animals, compromising the expected fertility. This new packaging device allows the consumer to be sure that he will use the inseminating dose as prepared by the Approved Insemination Center, which could increase the fertility results by strictly following the recommendations of the inseminating doses.
El dispositivo flexible consiste en un conducto de naturaleza preferentemente plástica de entre 1 y 5 mm. de diámetro para envasar semen que presenta una pluralidad de cuerpos rectos, unidos a través de una zona que comprende una pared plegable y flexible, en forma de fuelle o acordeón, que permite dejar el dispositivo completamente recto cuando se va a utilizar el contenido tras la descongelación, o con forma zigzagueante o flexionada durante su llenado y conservación. The flexible device consists of a conduit of a preferably plastic nature between 1 and 5 mm. in diameter for semen packaging that has a plurality of straight bodies, joined through an area that comprises a flexible and flexible wall, in the form of a bellows or accordion, which allows the device to be left completely straight when the content is to be used after the defrosting, or zigzagging or flexed during filling and preservation.
En concreto, este dispositivo para envasar semen comprende entre 2 y 7 partes rectas, de entre 10 y 20 cm de longitud, unidas por las citadas zonas plegables, lo que permite decidir qué volumen total será utilizado en la congelación, ya que da la posibilidad de cortar aquella zona que no se desea utilizar, manteniendo la característica de hacer indivisible la dosis inseminante. Specifically, this device for semen packaging comprises between 2 and 7 straight parts, between 10 and 20 cm in length, joined by the aforementioned folding areas, which allows to decide what total volume will be used in freezing, since it gives the possibility of cutting that area that you do not want to use, maintaining the characteristic of making the inseminant dose indivisible.
Otro elemento destacable de la presente invención es que el cierre del dispositivo se consigue mediante el sellado en al menos uno de sus extremos con doble algodón y polivinilpirrolidona para garantizar su cierre al contacto con el líquido, estando de manera normal previo al uso o tras su uso abierto en ambos extremos. Another remarkable element of the present invention is that the closure of the device is achieved by sealing at least one of its ends with double cotton and polyvinyl pyrrolidone to ensure its closure upon contact with the liquid, being normally prior to use or after its use. Open use at both ends.
Adicionalmente, el dispositivo incorpora un rigidizador o una ligera conexión plástica entre los diferentes cuerpos o zonas rectas que lo componen para evitar que dicho envase pierda su forma durante el proceso de manipulación, llenado o descongelación. Se ha de tener en cuenta que, a lo largo de la descripción y las reivindicaciones, el término “comprende” y sus variantes no pretenden excluir otras características técnicas o elementos adicionales. Además, con el objeto de completar la descripción y de ayudar a una mejor comprensión de las características del invento, se presenta un juego de figuras y dibujos en donde con carácter ilustrativo y no limitativo se representa lo siguiente: Additionally, the device incorporates a stiffener or a slight plastic connection between the different bodies or straight areas that compose it to prevent said container from losing its shape during the process of handling, filling or defrosting. It should be borne in mind that, throughout the description and the claims, the term "comprises" and its variants are not intended to exclude other technical characteristics or additional elements. In addition, in order to complete the description and to help a better understanding of the features of the invention, a set of figures and drawings is presented, where the following is illustrated and not limited to:
Figura 1. Esquema de un dispositivo flexible para congelación de esperma realizado de acuerdo con el objeto de la presente invención, cuando dicho dispositivos esta plegado o en forma zigzagueante. Figure 1. Scheme of a flexible device for freezing sperm made in accordance with the object of the present invention, when said devices are folded or in a zigzag shape.
Figura 2. Detalle ampliado de la unión de plástico entre los diferentes cuerpos que conforman el dispositivo flexible, y del sistema de flexión utilizado para conectar los diferentes cuerpos del dispositivo y que permite que este pueda ser conservado en posición plegada o pueda alargarse para su uso directo. Figure 2. Expanded detail of the plastic joint between the different bodies that make up the flexible device, and the flexion system used to connect the different bodies of the device and which allows it to be kept in a folded position or can be extended for use direct.
Figura 3. Esquema de un dispositivo flexible para congelación de esperma realizado de acuerdo con el objeto de la presente invención cuando dicho dispositivo está recto. Figure 3. Scheme of a flexible device for freezing sperm made in accordance with the object of the present invention when said device is straight.
Descripción detallada de los dibujos Detailed description of the drawings
En las Figuras 1 y 2 se observa una realización preferente de la invención donde el dispositivo está con forma plegada o zigzagueante, y en la Figura 3 se observa una disposición de ese mismo dispositivo completamente recta. En todo caso, el dispositivo flexible para la congelación de dosis seminales se puede observar que consiste en un conducto de naturaleza plástica que comprende una pluralidad de cuerpos rectos (1) unidos en sus extremos por unas uniones (2) con paredes plegables y flexibles en forma de fuelle que son precisamente las que permiten al dispositivo tener una configuración plegada (Fig.1 ) o recta (Fig.3), y que se cierra mediante la utilización de un tapón (3) al menos en uno de sus extremos. In Figures 1 and 2 a preferred embodiment of the invention is observed where the device is in a folded or zigzag shape, and in Figure 3 an arrangement of that same device is observed completely straight. In any case, the flexible device for freezing seminal doses can be seen to consist of a conduit of a plastic nature comprising a plurality of straight bodies (1) joined at their ends by joints (2) with folding and flexible walls in bellows shape that are precisely those that allow the device to have a folded (Fig. 1) or straight (Fig. 3) configuration, and that is closed by using a plug (3) at least at one of its ends.
Una realización preferente de la invención consiste en un conducto con 5 cuerpos rectos (1), teniendo dichos cuerpos rectos un diámetro de 2 mm, y una longitud de 13 cm; en el que el tapón (3) está constituido por un doble algodón y polivinilpirrolidona y se dispone a unos 2 cm del extremo del conducto; y donde, de cara a que se mantenga la forma plegada entre los diferentes cuerpos rectos (1), se dispone de una pluralidad de rigidizadores (4) o unas ligeras conexiones plásticas que fijan dichos cuerpos rectos (1). A preferred embodiment of the invention consists of a conduit with 5 straight bodies (1), said straight bodies having a diameter of 2 mm, and a length of 13 cm; wherein the plug (3) is constituted by a double cotton and polyvinylpyrrolidone and is disposed about 2 cm from the end of the duct; and where, in order to maintain the folded shape between the different straight bodies (1), there is a plurality of stiffeners (4) or light plastic connections that fix said straight bodies (1).

Claims

REIVINDICACIONES
1.- Dispositivo flexible para la congelación de dosis seminales, destinado a un uso reproductivo preferentemente en animales, en el que el dispositivo es un conducto de naturaleza plástica que se caracteriza por que comprende una pluralidad de cuerpos rectos (1) conectados en sus extremos por unas uniones (2) con paredes plegables y flexibles en forma de fuelle que le permite al dispositivo tener una configuración recta o plegada, y que se cierra mediante la utilización de un tapón (3) al menos en uno de sus extremos. 1.- Flexible device for freezing seminal doses, intended for reproductive use, preferably in animals, in which the device is a conduit of a plastic nature characterized by comprising a plurality of straight bodies (1) connected at its ends by joints (2) with folding and flexible bellows-shaped walls that allows the device to have a straight or folded configuration, and which is closed by using a plug (3) at least at one of its ends.
2.- Dispositivo flexible para la congelación de dosis seminales, según la reivindicación 1 , que se caracteriza por que el número de partes rectas del dispositivo se encuentra comprendido entre 2 y 7. 2. Flexible device for freezing seminal doses according to claim 1, characterized in that the number of straight parts of the device is between 2 and 7.
3.- Dispositivo flexible para la congelación de dosis seminales, según la reivindicación 1 , que se caracteriza por que el diámetro del conducto de las partes rectas del dispositivo se encuentra comprendido entre 1 y 5 mm. 3. Flexible device for freezing seminal doses according to claim 1, characterized in that the diameter of the conduit of the straight parts of the device is between 1 and 5 mm.
4 Dispositivo flexible para la congelación de dosis seminales, según la reivindicación 1 , que se caracteriza por que la longitud del conducto de las partes rectas del dispositivo se encuentra comprendido entre 10 y 20 cm. 4 Flexible device for freezing seminal doses according to claim 1, characterized in that the length of the duct of the straight parts of the device is between 10 and 20 cm.
5.- Dispositivo flexible para la congelación de dosis seminales, según la reivindicación 1 , que se caracteriza por que el tapón de sellado, en al menos uno de los extremos del dispositivo, está constituido de un doble algodón y polivinilpirrolidona. 5. Flexible device for freezing seminal doses, according to claim 1, characterized in that the sealing cap, at least one of the ends of the device, consists of a double cotton and polyvinylpyrrolidone.
6.- Dispositivo flexible para la congelación de dosis seminales, según la reivindicación 1 , que se caracteriza porque el dispositivo incorpora un rigidizador (4) plástico para el mantenimiento de la forma plegada entre los diferentes cuerpos rectos. rein 6. Flexible device for freezing seminal doses, according to claim 1, characterized in that the device incorporates a plastic stiffener (4) for maintaining the folded form between the different straight bodies. rein
PCT/ES2019/070152 2018-03-07 2019-03-08 Flexible device for freezing semen doses WO2019170951A1 (en)

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ES201830304U ES1208736Y (en) 2018-03-07 2018-03-07 FLEXIBLE DEVICE FOR FREEZING WEEKLY DOSE
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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5916144A (en) * 1995-05-09 1999-06-29 The Curators Of The University Of Missouri System for introducing a fluid into the uterus of an animal
GB2342912A (en) * 1998-05-20 2000-04-26 Imv Technologies Bag for packaging liquid substances for animal artificial insemination
EP1066802A2 (en) * 1999-07-09 2001-01-10 Livestock Improvement Association of Japan Inc. Injector of sperm or of a fertilized ovum into a domestic animal and method of operation thereof
US20020151765A1 (en) * 2000-01-03 2002-10-17 Javier Gil Pascual Artificial insemination device for pigs
WO2002085110A1 (en) * 2001-04-18 2002-10-31 Kabushikikaisha Kitazato Supply Egg freezing preservation tool, and cylindrical member holding tool
US20150282916A1 (en) * 2013-07-03 2015-10-08 Brad K. Stroud Method, apparatus and kit for artificial insemination of bovine

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5916144A (en) * 1995-05-09 1999-06-29 The Curators Of The University Of Missouri System for introducing a fluid into the uterus of an animal
GB2342912A (en) * 1998-05-20 2000-04-26 Imv Technologies Bag for packaging liquid substances for animal artificial insemination
EP1066802A2 (en) * 1999-07-09 2001-01-10 Livestock Improvement Association of Japan Inc. Injector of sperm or of a fertilized ovum into a domestic animal and method of operation thereof
US20020151765A1 (en) * 2000-01-03 2002-10-17 Javier Gil Pascual Artificial insemination device for pigs
WO2002085110A1 (en) * 2001-04-18 2002-10-31 Kabushikikaisha Kitazato Supply Egg freezing preservation tool, and cylindrical member holding tool
US20150282916A1 (en) * 2013-07-03 2015-10-08 Brad K. Stroud Method, apparatus and kit for artificial insemination of bovine

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ES1208736Y (en) 2018-06-21

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