Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
  • C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
  • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
  • C07D211/36—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
  • C07D211/56—Nitrogen atoms
  • C07D211/58—Nitrogen atoms attached in position 4
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
  • A61K31/445—Non condensed piperidines, e.g. piperocaine
  • A61K31/4468—Non condensed piperidines, e.g. piperocaine having a nitrogen directly attached in position 4, e.g. clebopride, fentanyl
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
  • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
  • A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/20—Hypnotics; Sedatives
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/22—Anxiolytics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/24—Antidepressants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P9/00—Drugs for disorders of the cardiovascular system
  • A61P9/12—Antihypertensives
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
  • C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
  • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
  • C07D211/36—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
  • C07D211/40—Oxygen atoms
  • C07D211/44—Oxygen atoms attached in position 4
  • C07D211/46—Oxygen atoms attached in position 4 having a hydrogen atom as the second substituent in position 4

Definitions

• Cisapride is one of a class of compounds known as benzamide derivatives, the parent compound of which is metoclopramide.
• U.S. Pat. Nos. 4,962,115 and 5,057,525 disclose N-(3- hydroxy-4-piperidenyl) benzamides of cisapride. Van Daele discloses that these compounds, the pharmaceutically acceptable acid addition salts thereof and the stereochemically isomeric forms thereof, stimulate the motility of the gastrointestinal system.
• these benzamide derivatives have several prominent pharmacological actions.
• the prominent pharmacological activities of the benzamide derivatives are due to their effects on the neuronal systems which are modulated by the neurotransmitter serotonin.
• the role of serotonin, and thus the pharmacology of the benzamide derivatives, has been broadly implicated in a variety of conditions for many years. Thus, research has focused on locating the production and storage sites of serotonin as well as the location of serotonin receptors in the human body in order to determine the connection between these sites and various disease states or conditions.
• serotonin a major site of production and storage of serotonin is the enterochromaffin cell of the gastrointestinal mucosa. It was also discovered that serotonin has a powerful stimulating action on intestinal motility by stimulating intestinal smooth muscle, speeding intestinal transit, and decreasing absorption time, as in diarrhea. This stimulating action is also associated with nausea and vomiting.
• benzamide derivatives are effective anti-emetic agents and are commonly used to control vomiting during cancer chemotherapy or radiotherapy, especially when highly emetogenic compounds such as cisplatin are used.
• This action is almost certainly the result of the ability of the compounds to block the actions of serotonin (5HT) at specific sites of action, called the 5HT3 -receptor, which was classically designated in the scientific literature as the serotonin M-receptor.
• Chemotherapy and radiation therapy may induce nausea and vomiting by the release of serotonin from damaged enterochromaffin cells in the gastrointestinal tract.
• a second prominent action of the benzamide derivatives is in augmenting gastrointestinal smooth muscle activity from the esophagus through the proximal small bowel, thus accelerating esophageal and small intestinal transit as well as facilitating gastric emptying and increasing lower esophageal sphincter tone (Decktor et al., Eur. J. Pharmacol. 147: 313-316, 1988).
• the benzamide derivatives are not cholinergic receptor agonists per se, the aforementioned smooth muscle effects may be blocked by muscarinic receptor blocking agents such as atropine or neuronal transmission inhibitors of the tetrodotoxin type which affect sodium channels.
• Cisapride is presently used primarily to treat gastroesophageal reflux disease. This disease is characterized as the backward flow of the stomach contents into the esophagus.
• gastroesophageal reflux disease is characterized as the backward flow of the stomach contents into the esophagus.
• One of the most important factors in the pathogenesis of gastroesophageal reflux disease is a reduction in the pressure barrier due to the failure of the lower esophageal sphincter. Failure of the lower esophageal sphincter can arise due to a low basal pressure, sphincter relaxation, or to a non-compensated increase in intragastric pressure.
• Cisapride is thought to strengthen the anti-reflux barrier and improve esophageal clearance by increasing the lower esophageal sphincter pressure and enhancing peristaltic contractions. Because of its activity as a prokinetic agent, cisapride would also appear to be useful to treat dyspepsia, gastroparesis, constipation, post-operative ileus, and intestinal pseudo- obstruction.
• Dyspepsia is a condition characterized by an impairment of the power or function of digestion that can arise as a symptom of a primary gastrointestinal dysfunction or as a complication due to other disorders such as appendicitis, gallbladder disturbances, or malnutrition.
• Gastroparesis is a paralysis of the stomach brought about by a motor abnormality in the stomach or as a complication of diseases such as diabetes, progressive systemic sclerosis, anorexia nervosa or myotonic dystrophy.
• Constipation is a condition characterized by infrequent or difficult evacuation of feces resulting from conditions such as lack of intestinal muscle tone or intestinal spasticity.
• Post-operative ileus is an obstruction in the intestine due to a disruption in muscle tone following surgery. Intestinal pseudo- obstruction is a condition characterized by constipation, colicky pain, and vomiting, but without evidence of physical obstruction.
• Drug toxicity is an important consideration in the treatment of humans and animals. Toxic side effects (adverse effects) resulting from the administration of drugs include a variety of conditions which range from low grade fever to death. Drug therapy is justified only when the benefits of the treatment protocol outweigh the potential risks associated with the treatment. The factors balanced by the practitioner include the qualitative and quantitative impact of the drug to be used as well as the resulting outcome if the drug is not provided to the individual. Other factors considered include the physical condition of the patient, the disease stage and its history of progression, and any known adverse effects associated with a drug. Drug elimination is typically the result of metabolic activity upon the drug and the subsequent excretion of the drug from the body. Metabolic activity can take place within the vascular supply and/or within cellular compartments or organs.
• the liver is a principal site of drug metabolism.
• the metabolic process can be categorized into synthetic and nonsynthetic reactions.
• nonsynthetic reactions the drug is chemically altered by oxidation, reduction, hydrolysis, or any combination of the aforementioned processes. These processes are collectively referred to as Phase I reactions.
• Phase II reactions also known as synthetic reactions or conjugations
• the parent drug, or intermediate metabolites thereof are combined with endogenous substrates to yield an addition or conjugation product.
• Metabolites formed in syntlietic reactions are, typically, more polar and biologically inactive. As a result, these metabolites are more easily excreted via the kidneys (in urine) or the liver (in bile).
• Synthetic reactions include glucuronidation, amino acid conjugation, acetylation, sulfoconjugation, and methylation.
• More than 90% of a dose of cisapride is metabolized by oxidative N-dealkylation at the piperidine nitrogen or by aromatic hydroxylation occurring on either the 4- fluorophenoxy or benzamide rings.
• cisapride The administration of cisapride to a human has been found to cause adverse effects including, CNS disorders, increased systolic pressure, interactions with other drugs, diarrhea, and abdominal cramping. Further, it has been reported that intravenous administration of cisapride demonstrates the occurrence of additional adverse (side) effects not experienced after oral administration of cisapride (Stacher et al. [1987] Digestive Diseases and Sciences 32(11): 1223-1230). It is believed that these side effects are caused by the metabolites which result from the oxidative dealkylation or aromatic hydroxylation of the compound which occurs in the cytochrome P-450 detoxification system. Between July 1993 and December 1999, cisapride (PROPULSID, Janssen
• compositions of the subject invention comprise esterifled cisapride derivatives. These compositions possess potent activity in treating gastroesophageal reflux disease and substantially reduce adverse effects associated with the administration of cisapride. These adverse effects include, but are not limited to, diarrhea, abdominal cramping and elevations of blood pressure and heart rate.
• novel compositions of the subject invention are useful in treating emesis and other conditions, including but not limited to dyspepsia, gastroparesis, constipation, and intestinal pseudo-obstruction.
• adverse effects associated with the administration of cisapride are also reduced in these methods of treatment.
• the subject invention provides compounds which are readily metabolized by the physiological metabolic drug detoxification systems.
• the therapeutic compounds of the subject invention contain a moiety, which does not detract from the ability of these compounds to provide a therapeutic benefit, but which makes these compounds more susceptible to degradation by hydrolases.
• compounds which contain an ester group making them susceptible to degradation by serum and/or cytosolic esterases thereby avoiding the cytochrome P-450 drug detoxification system associated with adverse effects caused by cisapride and reducing the incidence of adverse events.
• the subject invention further provides methods of treatment comprising the administration of these compounds to individuals in need of treatment for gastroesophageal reflux disease and related conditions.
• the therapeutic compounds of the subject invention are stable in storage and provide for safer metabolism of the drugs as compared to other drugs which are available for treatment of gastroesophageal reflux, dyspepsia, gastroparesis, constipation, post-operative ileus, and intestinal pseudo-obstruction; therefore, the compounds of the subject invention can be used with a lower incidence of side effects and toxicity.
• the subject invention pertains to the breakdown products which are formed when the therapeutic compounds of the subject invention are acted upon by hydrolases. These breakdown products can be used as described herein to monitor the clearance of the therapeutic compounds from a patient.
• the subject invention provides methods for synthesizing the therapeutic compounds of the subject invention.
• the subject invention provides novel compounds which are more easily metabolized by the metabolic drug detoxification systems. This invention is also drawn to methods of treating disorders, such as gastroesophageal reflux disease, and related conditions. Specifically, the subject invention provides analogs of cisapride which have been designed to be more susceptible to degradation by hydrolases, particularly serum and/or cytosolic esterases and methods of treatment comprising the administration of these analogs to individuals.
• the therapeutic compounds of the subject invention are stable in storage but have a relatively short half-life in the physiological environment; therefore, the compounds of the subject invention can be used with a lower incidence of side effects and toxicity.
• therapeutic compounds are provided which are useful in the treatment of gastroesophageal reflux disease and which contain a moiety, such as an ester group, which is susceptible to degradation by hydrolases, thereby breaking down the compound and facilitating its efficient removal from the treated individual.
• the therapeutic compounds are metabolized by the Phase I drug detoxification system.
• a further aspect of the subject invention pertains to the breakdown products which are produced when the therapeutic compounds of the subject invention are acted upon by a hydrolase.
• the presence of these breakdown products in the urine or serum can be used to monitor the rate of clearance of the therapeutic compound from a patient.
• Degradation of the compounds of the subject invention by enzymes such as hydrolases is particularly advantageous for drug metabolism because these enzymes are ubiquitously distributed and their activity is not dependent on age, gender, or disease state to the same extent as oxidative hepatic drug metabolism.
• the subject invention further provides methods of treating disorders, such as gastroesophageal reflux disease comprising the administration of a therapeutically effective amount of cisapride analogs to an individual in need of treatment.
• disorders such as gastroesophageal reflux disease
• the subject invention provides esterifled cisapride analogs and pharmaceutical compositions of these esterifled compounds.
• the subject invention further provides materials and methods for the treatment of emesis and such other conditions, including but not limited to dyspepsia, gastroparesis, constipation, and intestinal pseudo-obstruction, while substantially reducing adverse effects associated with the administration of cisapride.
• therapeutic compounds are provided which are useful in the treatment of gastroesophageal reflux, dyspepsia, gastroparesis, constipation, post-operative ileus, and intestinal pseudo-obstruction and which contain an ester group which is acted upon by esterases thereby breaking down the compound and facilitating its efficient removal from the treated individual.
• the subject invention further provides methods of synthesizing the unique and advantageous therapeutic compounds of the subject invention.
• ester linkage may be introduced into the compound at a site which is convenient in the manufacturing process for the target drug.
• sensitivity of the ester linkage may be manipulated by the addition of side groups which hinder or promote the hydrolytic activity of the hydrolases or esterases responsible for cleaving the drug at the ester locus. Methods of adding such side groups, as well as the side groups themselves, are well known to the skilled artisan and can be readily carried out utilizing the guidance provided herein.
• the chemical synthesis of the disclosed analogs of cisapride can be performed by the method described in European Patent Application No. 0,076,530 A2 published Apr. 13, 1983, U.S. Pat.
• X is O orN
• Ri and X are in the cis- or trans-configuration;
• R 3 is H or C 1 - 3 alkyl (if X is an oxygen atom, then R 3 does not exist);
• X is -CH(OH)-, -NH-, -S-, -O-, or a direct bond;
• m is an integer from 0 to 4 inclusive;
• p is 0 or 1 ;
• R 9 and R lo are, independently, H, C M alkyl, or R R ⁇ ) are linked and together form a 5- or a 6-membered cycloalkyl ring;
• Y is H, d- 14 alkyl or cycloalkyl optionally substituted by 1 or more (2 to 8) heteroatoms selected from the group consisting of O; N; S; or aryl or heteroaryl optionally substituted by 1 or more (2 to 8) halogen atoms, C alkyl, hydroxy, cyano, amino, alkylamino, dialkylamino, trifluoromethyl, -COOH, or -COOC M alkyl (when Y is hydrogen, the compounds can also be quaternary ammonium complexes such as tetrabutyl or tetraethylammonium and trigonellinium).
• the structure of Formula I has at least 2 asymmetric centers at the 3- and 4-positions of the piperidine ring (carbon atoms bearing the Ri and R 2 groups). Substituents on the piperidinyl ring can have the cis- or the trans- configuration. Accordingly, the subject invention includes the 4 individual enantiomers associated with these 2 carbon centers, namely the 3R,4R; 3S,4S; 3R,4S; and 3S,4R conformations.
• Preferred compounds are those wherein 4 , R 5 , and R 6 are, independently, selected from the group consisting of halo, amino, mono- and dialkylamino, and lower alkyloxy.
• Particularly preferred compounds are those wherein R 4 is methoxy, R 5 is amino or methylamino, and R 5 is chloro, in the 2-, 4-, and 5- position of the phenyl ring, respectively.
• R] and X are in the cis- conf ⁇ guration.
• Preferred compounds within the scope of this invention have the cis- configuration.
• Particularly preferred compounds of this invention have the following formulae:
• the compounds of Formula I can generally be prepared by the reaction of an amine of Formula (IV) with a carboxylic acid of Formula (V).
• Functional derivatives of the carboxylic acids shown as Formula (V) can also be used, as would be known to persons skilled in the art of synthetic chemistry. Suitable functional derivatives include acyl halides, anhydrides, and esters.
• the reaction conditions for mixing (IV) and (V) to produce (I) are well known conditions to the ordinary skilled synthetic chemist.
• the compounds of Formula I wherein Ri is hydrogen and the substituents in the 3- and 4- positions of the piperidine ring have the trans configuration, said compounds being represented by the Formula (la), can be prepared by reacting a 7-oxo-3- azabicyclo[4,l,0]heptane of Formula (VI) with a benzamide of Formula (VII). These compounds can be further alkylated in order to obtain a product wherein R 1 is other than hydrogen.
• the compounds of Formula (I) wherein the substituents in the 3- and 4- positions of the piperidine ring have the cis configuration, said compounds being represented by the Formula (lb), can be prepared by the reductive alkylation of a piperidone of Formula (VIII) with a benzamide of Formula (VII). This approach is applicable only when R 2 is hydrogen. Another approach, which is applicable whether R 2 is hydrogen or lower alkyl, is to react an amine of Formula (IX), having the 3- and 4- substituents of the piperidine ring in the cis configuration, with a carboxylic acid of Formula (V) or a suitable functional derivative thereof (an ester, an anhydride, or an acyl chloride for example).
• the compounds of this invention have therapeutic properties similar to those of the unmodified parent compounds. Accordingly, dosage rates and routes of administration of the disclosed compounds are similar to those already used in the art and known to the skilled artisan (see, for example, Physicians' Desk Reference, 54th Ed., Medical Economics Company, Mon vale, NJ, 2000).
• esterifled cisapride in the acute or chronic management of diseases and/or disorders described herein will vary with the severity of the condition to be treated, and the route of administration.
• the dose, and perhaps the dose frequency, will also vary according to the age, body weight, and response of the individual patient.
• the total daily dose range for esterifled cisapride, for the conditions described herein is from about 1 mg to about 200 mg, in single or divided doses.
• a daily dose range should be between about 5 mg to about 100 mg, in single or divided doses, while most preferably, a daily dose range should be between about 5 mg to about 75 mg, in single or divided doses.
• the doses are administered from 1 to 4 times a day.
• the therapy should be initiated at a lower dose, perhaps about 5 mg to about 10 mg, and increased up to about 50 mg or higher depending on the patient's global response. It is further recommended that children, and patients over 65 years, and those with impaired renal or hepatic function, initially receive low doses, and that they be titrated based on individual response(s) and blood level(s). It may be necessary to use dosages outside these ranges in some cases as will be apparent to those skilled in the art. Further, it is noted that the clinician or treating physician will know how and when to interrupt, adjust, or terminate therapy in conjunction with individual patient response.
• compositions of the subject invention can be formulated according to known methods for preparing pharmaceutically useful compositions. Formulations are described in detail in a number of sources which are well known and readily available to those skilled in the art. For example, Remington's Pharmaceutical Science by E.W. Martin describes formulations which can be used in connection with the subject invention. In general, the compositions of the subject invention are formulated such that an effective amount of the bioactive compound(s) is combined with a suitable carrier in order to facilitate effective administration of the composition.
• compositions of the subject invention include compositions such as suspensions, solutions and elixirs; aerosols; or carriers such as starches, sugars, microcrystalline cellulose, diluents, granulating agents, lubricants, binders, disintegrating agents, and the like, in the case of oral solid preparations (such as powders, capsules, and tablets) with the oral solid preparations being preferred over the oral liquid preparations.
• oral solid preparations such as powders, capsules, and tablets
• a preferred oral solid preparation is capsules.
• the most preferred oral solid preparation is tablets.
• Preferred amounts of active ingredient (i.e., an esterifled cisapride analog) in a solid dosage form are about 5 mg, 10 mg, and 25 mg.
• acceptable carriers can be either solid or liquid.
• Solid form preparations include powders, tablets, pills, capsules, cachets, suppositories and dispersible granules.
• a solid carrier can be one or more substances which may act as diluents, flavoring agents, solubilizers, lubricants, suspending agents, binders, preservatives, tablet disintegrating agents or encapsulating materials.
• the disclosed pharmaceutical compositions may be subdivided into unit doses containing appropriate quantities of the active component.
• the unit dosage form can be a packaged preparation, such as packeted tablets, capsules, and powders in paper or plastic containers or in vials or ampules.
• the unit dosage can be a liquid based preparation or formulated to be incorporated into solid food products, chewing gum, or lozenge.
• the compounds of the present invention may also be administered by controlled release means and/or delivery devices such as those described in U.S. Pat. Nos.: 3,845,770; 3,916,899; 3,536,809; 3,598,123; and 4,008,719, the disclosures of which are hereby incorporated by reference in their entirety.
• Any suitable route of administration may be employed for providing the patient with an effective dosage of esterifled cisapride.
• oral, rectal, parenteral (subcutaneous, intramuscular, intravenous), transdermal, and like forms of administration may be employed.
• Dosage forms include tablets, troches, dispersions, suspensions, solutions, capsules, patches, and the like.
• One embodiment of the invention provides a method of treating gastroesophageal reflux disease in a mammal, while substantially reducing the concomitant adverse effects associated with the administration of cisapride, which comprises administering to a human in need of such treatment, a therapeutically effective amount of esterifled cisapride, or a pharmaceutically acceptable salt thereof.
• a preferred embodiment is the treatment of gastroesophageal reflux disease in humans.
• Another embodiment of the invention provides a composition for the treatment of a human suffering from gastroesophageal reflux disease, which comprises a therapeutically effective amount of esterifled cisapride, or a pharmaceutically acceptable salt thereof.
• Yet another embodiment of the present invention provides a method of eliciting an anti-emetic effect in a mammal, while substantially reducing the adverse effects associated with the administration of cisapride, which comprises administering to a mammal in need of such anti-emetic therapy, a therapeutically effective amount of esterifled cisapride, or a pharmaceutically acceptable salt thereof.
• the mammal is a human.
• the present invention encompasses an anti-emetic composition for the treatment of a mammal in need of anti-emetic therapy, which comprises a therapeutically effective amount of esterifled cisapride, or a pharmaceutically acceptable salt thereof.
• a further aspect of the present invention includes a method of treating a condition caused by gastrointestinal motility dysfunction in a mammal which comprises administering to a mammal in need of treatment for gastrointestinal motility dysfunction, a therapeutically effective amount of esterifled cisapride, or a pharmaceutically acceptable salt thereof.
• Conditions caused by gastrointestinal motility dysfunction include, but are not limited to, dyspepsia, gastroparesis, constipation, post-operative ileus, and intestinal pseudo- obstruction.
• the mammal is a human.
• the observation that cisapride enters the central nervous system and binds to 5HT4 receptors indicates that cisapride may have centrally-mediated effects.
• Cisapride is a potent ligand at 5HT4 receptors, and these receptors are located in several areas of the central nervous system. Modulation of serotonergic systems has a variety of behavioral effects. According, the compounds of the subject invention can be used in the treatment of: 1) cognitive disorders, including but not limited to Alzheimer's disease; 2) behavioral disorders, including but not limited to schizophrenia, mania, obsessive-compulsive disorder, and psychoactive substance use disorders; 3) mood disorders, including but not limited to depression and anxiety; and 4) disorders of control of autonomic function, including but not limited to essential hypertension and sleep disorders.
• cognitive disorders including but not limited to Alzheimer's disease
• behavioral disorders including but not limited to schizophrenia, mania, obsessive-compulsive disorder, and psychoactive substance use disorders
• mood disorders including but not limited to depression and anxiety
• disorders of control of autonomic function including but not limited to essential hypertension and sleep disorders.
• the present invention also provides methods of treating cognitive, behavioral, mood, or autonomic function control disorders in a mammal comprising the administration of a therapeutically effective amount of esterifled cisapride, or a pharmaceutically acceptable salt thereof.
• the mammal is a human.
• pharmaceutically acceptable salts or "a pharmaceutically acceptable salt thereof refer to salts prepared from pharmaceutically acceptable non-toxic acids or bases including inorganic acids and bases and organic acids and bases. Since the compound of the present invention is basic, salts may be prepared from pharmaceutically acceptable non- toxic acids.
• Suitable pharmaceutically acceptable acid addition salts for the compound of the present invention include acetic, benzenesulfonic (besylate), benzoic, camphorsulfonic, citric, ethenesulfonic, fumaric, gluconic, glutamic, hydrobromic, hydrochloric, isethionic, lactic, maleic, malic, mandelic, methanesulfonic, mucic, nitric, pamoic, pantothenic, phosphoric, succinic, sulfuric, tartaric acid, p-toluenesulfonic, and the like.
• Preferred acid addition salts are the chloride and sulfate salts.
• esterifled cisapride analogs are administered as the free base.
• therapeutically effective amount means: 1) an amount sufficient to alleviate reflux disease, 2) an amount sufficient to alleviate nausea and vomiting, or 3) an . amount sufficient to alleviate a condition caused by gastrointestinal motility dysfunction.
• Therapeutically effective amounts of esterifled cisapride are encompassed by the above- described dosage amounts and dose frequency schedule.
• a "mammal” may be, for example, a mouse, rat, pig, horse, rabbit, goat, pig, cow, cat, dog, or human. In a preferred embodiment, the mammal is a human.
• the term "individual(s)" is defined as a single mammal to which is administered a compound of the present invention.
• the mammal may be a rodent, for example a mouse or rat, pig, horse, rabbit, goat, pig, cow, cat, dog, or human. In a preferred embodiment, the individual is a human.
• esterifled cisapride means therapeutic compounds of the subject invention which contain an ester group which does not detract from the ability of these compounds to provide a therapeutic benefit, but which makes these compounds more susceptible to degradation by hydrolases, particularly serum and/or cytosolic esterases, and which reduces the interaction of the cytochrome P-450 drug detoxification system with the cisapride compounds.
• Esterase mediated metabolism of the esterifled cisapride compounds reduces the role of the cytochrome P-450 drug detoxification system in cisapride metabolism and reduces or eliminates adverse effects caused by cisapride.
• abnormal effects includes, but is not limited to, gastrointestinal disorders such as diarrhea, abdominal cramping, and abdominal grumbling; tiredness; headache; increased systolic pressure; death; ventricular tachycardia; ventricular fibrillation; torsades de pointes; QT prolongation; increased heart rate; neurological and CNS disorders; and interaction of cisapride with other drugs given concurrently such as digoxin, diazepam, ethanol, acenocoumarol, cimetidine, ranitidine, paracetamol, and propranolol.
• stomach reflux disease means the incidence of, and the symptoms of, those conditions causing the backward flow of the stomach contents into the esophagus.
• eliciting an anti-emetic effect and "anti-emetic therapy” as used herein mean providing relief from or preventing the symptoms of nausea and vomiting induced spontaneously or associated with emetogenic cancer chemotherapy or irradiation therapy.
• treating a condition caused by gastrointestinal motility dysfunction means treating the symptoms and conditions associated with this disorder which include, but are not limited to, dyspepsia, gastroparesis, constipation, post-operative ileus, and intestinal pseudo-obstruction.
• prokinetic means the enhancement of peristalsis in, and thus the movement through the gastrointestinal tract.
• dispepsia as used herein means a condition characterized by an impairment of the power or function of digestion that can arise as a symptom of a primary gastrointestinal dysfunction or as a complication due to other disorders such as appendicitis, gallbladder disturbances, or malnutrition.
• gastroparesis means a paralysis of the stomach brought about by a motor abnormality in the stomach or as a complication of diseases such as diabetes, progressive systemic sclerosis, anorexia nervosa, or myotonic dystrophy.
• constipation means a condition characterized by infrequent or difficult evacuation of feces resulting from conditions such as lack of intestinal muscle tone or intestinal spasticity.
• post-operative ileus means an obstruction in the intestine due to a disruption in muscle tone following surgery.
• intestinal pseudo-obstruction means a condition characterized by constipation, colicky pain, and vomiting, but without evidence of physical obstruction.
• Preferred compounds of the subject invention have the Formula (lb) where the substituents at the 3- and 4- positions of the piperidine ring have the cis- configuration, Ri is methoxy, R 2 is hydrogen, R 4 is methoxy, R 5 is amino, R 6 is chloro in the 2-, 4-, and 5- positions of the benzamide ring, respectively.
• the common intermediate to these preferred compounds is compound 9 as described below.
• l-carbethoxy-4-piperidone 2 reacts with bromine in an inert solvent such as- dichloromethane to give high yields of l-carbethoxy-3-bromo-4-piperidone 2.
• the bromo compound 2 reacts with sodium methoxide in methanol to give l-carbethoxy-3-hydroxy- 4,4-dimethoxypiperidine 3, which in turn is alkylated to the corresponding 3 -methoxy analog 4 with iodomethane in dimethylformamide in the presence of sodium hydride.
• the ketal 4 is hydrolyzed to l-carbethoxy-3-methoxy-4-piperidone 5 by stirring in 1% sulfuric acid at room temperature.
• the amine 6 of cis-conflguration is then readily obtained by reductive alkylation of 5 with benzylamine in the presence of hydrogen gas and 10% Pd/C with a small amount of thiophene. Further hydrogenolysis of the benzyl moiety with Pd/C and no thiophene gives the primary amine 7.
• Compound 7 in turn reacts with the commercially available 4-amino-5-chloro-2-methoxybenzoic acid in the presence of DCC and dimethyammopyridine in dichloromethane to give the benzamide 8.
• Compound 8 is then hydrolyzed to the intermediate 9 with potassium hydroxide in ethanol/water.
• the intermediate 9 reacts with acrylic acid or an ester thereof in the presence of a base such as diethylamine to give the final compounds H (see diagram below).
• the compounds of Formula (I) wherein X is oxygen and Ri is methoxy can be prepared by reduction of a compound of Formula (VIII) with sodium borohydride in lower alkanol solvent, followed by coupling of the resulting alcohol (X) with a substituted benzoic acid of Formula (V) in the presence of a coupling reagent such as a dialkylcarbodiimide.
• Compounds of Formula I where L is CH 2 CHMeCOOR can be prepared by reacting the amine intermediate IX with methacrylic acid or an ester thereof, optionally in the presence of a base such as Triton B or triethylamine.
• Compounds of Formula I where L is CH 2 CMe 2 COOR can be prepared according to Katritzky et al., Synthesis (1989), 747 by reacting the benzotriazolylmethyl derivative of the amine intermediate IX with a 2-bromoisobutyric acid ester in the presence of zinc and trimethylsilyl chloride.
• Compounds of Formula I where L is CH 2 COOR are prepared by alkylating intermediate IX with bromoacetic acid or an ester thereof in the presence of a base such as potassium carbonate or triethylamine in an inert solvent such as tetrahydrofuran or dimethylformamide.
• Compounds of Formula I where L is (CH 2 ) 3 COOR can be made by alkylating intermediate IX with 4-bromobutyric acid or an ester thereof in the presence of a base such as potassium carbonate or triethylamine in an inert solvent such as tetrahydrofuran or dimethylformamide.
• a base such as potassium carbonate or triethylamine
• an inert solvent such as tetrahydrofuran or dimethylformamide.
• a segment of oesophagus obtained from Wistar derived male or female rats weighing 270 ⁇ 25 g and sacrificed by C0 2 overexposure is used.
• the tissue is placed under 1 g tension in a 10 mL bath containing 3 ⁇ M indomethacin and 1 ⁇ M ketanserin in Krebs solution pH 7.4 and at 32°C and submaximal tonic isometrically recorded contraction is induced by carbachol (1 ⁇ M).

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