WO2000071749A3 - Erkennungssystem zur untersuchung von molekülwechselwirkungen, seine herstellung und verwendung - Google Patents

Erkennungssystem zur untersuchung von molekülwechselwirkungen, seine herstellung und verwendung Download PDF

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Publication number
WO2000071749A3
WO2000071749A3 PCT/EP2000/004791 EP0004791W WO0071749A3 WO 2000071749 A3 WO2000071749 A3 WO 2000071749A3 EP 0004791 W EP0004791 W EP 0004791W WO 0071749 A3 WO0071749 A3 WO 0071749A3
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WO
WIPO (PCT)
Prior art keywords
detection system
component
utilization
bonded
region
Prior art date
Application number
PCT/EP2000/004791
Other languages
English (en)
French (fr)
Other versions
WO2000071749A2 (de
Inventor
Dirk Boekenkamp
Hans-Ulrich Hoppe
Petra Burgstaller
Dirk Konz
Uwe Woelk
Marc Pignot
Original Assignee
Aventis Res & Tech Gmbh & Co
Dirk Boekenkamp
Hoppe Hans Ulrich
Petra Burgstaller
Dirk Konz
Uwe Woelk
Marc Pignot
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Res & Tech Gmbh & Co, Dirk Boekenkamp, Hoppe Hans Ulrich, Petra Burgstaller, Dirk Konz, Uwe Woelk, Marc Pignot filed Critical Aventis Res & Tech Gmbh & Co
Priority to EEP200100616A priority Critical patent/EE200100616A/xx
Priority to IL14637100A priority patent/IL146371A0/xx
Priority to JP2000620126A priority patent/JP2003500066A/ja
Priority to CA002374438A priority patent/CA2374438A1/en
Priority to EP00941987A priority patent/EP1185704A2/de
Priority to AU56760/00A priority patent/AU5676000A/en
Publication of WO2000071749A2 publication Critical patent/WO2000071749A2/de
Publication of WO2000071749A3 publication Critical patent/WO2000071749A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Die Erfindung bezieht sich auf ein Erkennungssystem enthaltend: a) einen Träger (Komponente (a) und (b)), mindestens eine an den Träger gebundene Erkennungseinheit (Komponente (b)), vorzugsweise eine Nukleinsäure, wobei die genannte Erkennungseinheit (Komponente (b)), eine Region (A) mit einer konstanten Struktur und eine zu der Region (A) benachbarte Region (B) mit einer variablen Struktur enthält, auf ein Verfahren zur Auftrennung einzelner Bestandteile in einer Probe, bei dem an mindestens eine an einen Träger gebundene Erkennungseinheit (Komponente (b)) unter geeigneten Bedingungen mindestens ein Bestandteil der Probe gebunden wird, und auf die Verwendung des erfindungsgemäßen Erkennungssystems und des erfindungsgemäßen Verfahrens zum Auffinden und/oder zur Identifizierung bzw. Charakterisierung von Nukleinsäuren und/oder Proteinen einer Probe bzw. zum Auffinden und/oder zur Identifizierung von zellulären oder künstlichen Bindepartnern.
PCT/EP2000/004791 1999-05-25 2000-05-25 Erkennungssystem zur untersuchung von molekülwechselwirkungen, seine herstellung und verwendung WO2000071749A2 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EEP200100616A EE200100616A (et) 1999-05-25 2000-05-25 Detekteerimissüsteem, selle valmistamismeetod, meetod üksikute komponentide fraktsioneerimiseks ja identifitseerimiseks, meetod sidumispartneri interaktsioonide identifitseerimiseks ja konjugaat
IL14637100A IL146371A0 (en) 1999-05-25 2000-05-25 Detection system for studying molecular interactions and its preparation and use
JP2000620126A JP2003500066A (ja) 1999-05-25 2000-05-25 分子相互作用の研究用検出システムおよびその製造および使用
CA002374438A CA2374438A1 (en) 1999-05-25 2000-05-25 Detection system for studying molecular interactions and its preparationand use
EP00941987A EP1185704A2 (de) 1999-05-25 2000-05-25 Erkennungssystem zur untersuchung von molekülwechselwirkungen, seine herstellung und verwendung
AU56760/00A AU5676000A (en) 1999-05-25 2000-05-25 Detection system for analyzing molecular interactions, production and utilization thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19923966A DE19923966C2 (de) 1999-05-25 1999-05-25 Erkennungssystem zur Auftrennung von Probenbestandteilen, seine Herstellung und Verwendung
DE19923966.5 1999-05-25

Publications (2)

Publication Number Publication Date
WO2000071749A2 WO2000071749A2 (de) 2000-11-30
WO2000071749A3 true WO2000071749A3 (de) 2001-09-07

Family

ID=7909147

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/EP2000/002938 WO2000071747A2 (de) 1999-05-25 2000-04-01 Erkennungssystem zur auftrennung von probenbestandteilen, seine herstellung und verwendung
PCT/EP2000/004791 WO2000071749A2 (de) 1999-05-25 2000-05-25 Erkennungssystem zur untersuchung von molekülwechselwirkungen, seine herstellung und verwendung

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/002938 WO2000071747A2 (de) 1999-05-25 2000-04-01 Erkennungssystem zur auftrennung von probenbestandteilen, seine herstellung und verwendung

Country Status (10)

Country Link
EP (1) EP1185704A2 (de)
JP (1) JP2003500066A (de)
CN (1) CN1413262A (de)
AU (2) AU3659100A (de)
CA (1) CA2374438A1 (de)
CZ (1) CZ20014210A3 (de)
DE (1) DE19923966C2 (de)
EE (1) EE200100616A (de)
IL (1) IL146371A0 (de)
WO (2) WO2000071747A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1208224A2 (de) * 1999-03-22 2002-05-29 Paul Cullen Nukleinsäurekombination
US20040161741A1 (en) * 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
JP2003299489A (ja) * 2002-02-08 2003-10-21 Mitsubishi Chemicals Corp 核酸構築物
JPWO2005012902A1 (ja) * 2003-07-31 2006-10-05 有限会社ジーン・フィールド 有用タンパク質のスクリーニング方法

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993017126A1 (en) * 1992-02-19 1993-09-02 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
WO1997031256A2 (en) * 1996-02-09 1997-08-28 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
WO1998031700A1 (en) * 1997-01-21 1998-07-23 The General Hospital Corporation Selection of proteins using rna-protein fusions
US5849486A (en) * 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
DE19741716A1 (de) * 1997-09-22 1999-03-25 Hoechst Ag Adressierbares modulares Erkennungssystem, seine Herstellung und Verwendung
WO1999051773A1 (en) * 1998-04-03 1999-10-14 Phylos, Inc. Addressable protein arrays
WO2001016352A1 (en) * 1999-08-27 2001-03-08 Phylos, Inc. Methods for encoding and sorting in vitro translated proteins

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5700637A (en) * 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
US5795714A (en) * 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
AU2253397A (en) * 1996-01-23 1997-08-20 Affymetrix, Inc. Nucleic acid analysis techniques
WO1999023492A1 (en) * 1997-10-31 1999-05-14 Sarnoff Corporation Method for enhancing fluorescence

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993017126A1 (en) * 1992-02-19 1993-09-02 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5849486A (en) * 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
WO1997031256A2 (en) * 1996-02-09 1997-08-28 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
WO1998031700A1 (en) * 1997-01-21 1998-07-23 The General Hospital Corporation Selection of proteins using rna-protein fusions
DE19741716A1 (de) * 1997-09-22 1999-03-25 Hoechst Ag Adressierbares modulares Erkennungssystem, seine Herstellung und Verwendung
WO1999051773A1 (en) * 1998-04-03 1999-10-14 Phylos, Inc. Addressable protein arrays
WO2001016352A1 (en) * 1999-08-27 2001-03-08 Phylos, Inc. Methods for encoding and sorting in vitro translated proteins

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NIEMEYER C M ET AL: "OLIGONUCLEOTIDE-DIRECTED SELF-ASSEMBLY OF PROTEINS: SEMISYNTHETIC DNA-STREPTAVIDIN HYBRID MOLECULES AS CONNECTORS FOR THE GENERATION OF MACROSCOPIC ARRAYS AND THE CONSTRUCTION OF SUPRAMOLECULAR BIOCONJUGATES", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 22, no. 25, 1994, pages 5530 - 5539, XP000645135, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
EP1185704A2 (de) 2002-03-13
AU3659100A (en) 2000-12-12
EE200100616A (et) 2003-02-17
CN1413262A (zh) 2003-04-23
AU5676000A (en) 2000-12-12
CA2374438A1 (en) 2000-11-30
JP2003500066A (ja) 2003-01-07
IL146371A0 (en) 2002-07-25
WO2000071747A3 (de) 2001-06-14
DE19923966C2 (de) 2003-04-24
DE19923966A1 (de) 2000-11-30
WO2000071747A2 (de) 2000-11-30
CZ20014210A3 (cs) 2002-06-12
WO2000071749A2 (de) 2000-11-30

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