WO1999061623A3 - Genetisches verfahren zur herstellung von riboflavin - Google Patents

Genetisches verfahren zur herstellung von riboflavin Download PDF

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Publication number
WO1999061623A3
WO1999061623A3 PCT/EP1999/003196 EP9903196W WO9961623A3 WO 1999061623 A3 WO1999061623 A3 WO 1999061623A3 EP 9903196 W EP9903196 W EP 9903196W WO 9961623 A3 WO9961623 A3 WO 9961623A3
Authority
WO
WIPO (PCT)
Prior art keywords
riboflavin
organisms
nucleic acid
seq
genetic method
Prior art date
Application number
PCT/EP1999/003196
Other languages
English (en)
French (fr)
Other versions
WO1999061623A2 (de
Inventor
Henning Althoefer
Harald Seulberger
Oskar Zelder
Doval Jose Luis Revuelta
Original Assignee
Basf Ag
Henning Althoefer
Harald Seulberger
Oskar Zelder
Doval Jose Luis Revuelta
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag, Henning Althoefer, Harald Seulberger, Oskar Zelder, Doval Jose Luis Revuelta filed Critical Basf Ag
Priority to KR1020007013352A priority Critical patent/KR20010043867A/ko
Priority to EP99924924A priority patent/EP1082438A2/de
Priority to JP2000551007A priority patent/JP2002516108A/ja
Priority to AU41409/99A priority patent/AU4140999A/en
Publication of WO1999061623A2 publication Critical patent/WO1999061623A2/de
Publication of WO1999061623A3 publication Critical patent/WO1999061623A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P25/00—Preparation of compounds containing alloxazine or isoalloxazine nucleus, e.g. riboflavin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Die vorliegende Erfindung betrifft ein genetisches Verfahren zur Herstellung von Riboflavin. Durch die spezielle Auswahl von Genen der Riboflavinbiosynthese bzw. deren Kombination in Organismen, speziell in Bakterien, Pilzen, Hefen und Pflanzen und deren Expression, wird die Riboflavinproduktion in diesen Organismen gesteigert. Die Erfindung betrifft weiterhin Nukleinsäurefragment enthaltend Gene mit den Sequenzen SEQ ID No. 1, SEQ ID No. 3 oder SEQ ID No. 5 oder deren funktionellen Äquivalente, Expressionsvektoren enthaltend die Nukleinsäurefragmente und Organismen enthaltend mindestens ein Nukleinsäurefragment oder mindestens einen Vektor.
PCT/EP1999/003196 1998-05-28 1999-05-10 Genetisches verfahren zur herstellung von riboflavin WO1999061623A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
KR1020007013352A KR20010043867A (ko) 1998-05-28 1999-05-10 리보플라빈을 생산하는 유전적 방법
EP99924924A EP1082438A2 (de) 1998-05-28 1999-05-10 Genetisches verfahren zur herstellung von riboflavin
JP2000551007A JP2002516108A (ja) 1998-05-28 1999-05-10 リボフラビンの遺伝的製法
AU41409/99A AU4140999A (en) 1998-05-28 1999-05-10 Genetic method for producing riboflavin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19823834A DE19823834A1 (de) 1998-05-28 1998-05-28 Genetisches Verfahren zur Herstellung von Riboflavin
DE19823834.7 1998-05-28

Publications (2)

Publication Number Publication Date
WO1999061623A2 WO1999061623A2 (de) 1999-12-02
WO1999061623A3 true WO1999061623A3 (de) 2000-01-27

Family

ID=7869161

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/003196 WO1999061623A2 (de) 1998-05-28 1999-05-10 Genetisches verfahren zur herstellung von riboflavin

Country Status (9)

Country Link
EP (1) EP1082438A2 (de)
JP (1) JP2002516108A (de)
KR (1) KR20010043867A (de)
CN (1) CN1303434A (de)
AU (1) AU4140999A (de)
DE (1) DE19823834A1 (de)
ID (1) ID27073A (de)
RU (1) RU2000133310A (de)
WO (1) WO1999061623A2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TR200103706T2 (tr) 1999-06-25 2002-10-21 Basf Aktiengesellschaft Karbon metabolizma ve enerji üretimindeki proteinleri kodlayan corynebacterium glutamicum genleri.
PL359501A1 (en) 1999-07-01 2004-08-23 Basf Aktiengesellschaft Orynebacterium glutamicum
DE1246922T1 (de) 1999-07-01 2003-03-20 Basf Ag Für proteine des phosphoenolpyruvat:zucker phosphotransferase systems kodierende gene aus corynebacterium glutamicum
WO2002006448A2 (en) * 2000-07-14 2002-01-24 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
US7009045B2 (en) 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
DE10046870A1 (de) 2000-09-20 2002-03-28 Basf Ag Verfahren zur Veränderung des Genoms von Corynebakterien
ATE421577T1 (de) 2001-04-04 2009-02-15 Genencor Int Entkoppelte anabole und katabole stoffwechselwege in wirtszellen
EP1383903B1 (de) 2001-04-04 2008-09-24 Genencor International, Inc. Verfahren zur herstellung von ascorbinsäurezwischenprodukten in wirtszellen
WO2003012101A1 (de) * 2001-07-27 2003-02-13 Basf Aktiengesellschaft Neue genprodukte aus ashbya gossypii, die mit den mechanismen der signaltransduktion und isnbesondere mit der verbesserung der vitamin b2-produktion assoziiert sind
CN1604964A (zh) * 2001-08-23 2005-04-06 巴斯福股份公司 棉桃阿舒氏囊霉的酶
DE10159396A1 (de) * 2001-12-04 2003-06-12 Basf Ag Genetische Stammoptimierung zur verbesserten Herstellung von Riboflavin
DE10209363A1 (de) * 2002-03-02 2003-09-11 Basf Ag Verfahren zur Herstellung von Riboflavin
JP5565992B2 (ja) * 2005-09-28 2014-08-06 興人ライフサイエンス株式会社 酵母キャンディダ・ユティリスの形質転換法
JP5498651B2 (ja) * 2006-07-28 2014-05-21 花王株式会社 ジピコリン酸又はその塩の製造方法
CN104531745B (zh) * 2014-12-09 2018-05-04 江南大学 一种新型抗性质粒的构建及其在核黄素生产菌中的运用
CN113755551A (zh) * 2021-09-30 2021-12-07 天津科技大学 一种提高核黄素产量的发酵方法
CN114317557B (zh) * 2022-01-06 2023-07-07 河南农业大学 玉米ZmRIBA1基因在高赖氨酸玉米育种中的应用
CN116463305B (zh) * 2023-06-15 2023-10-17 北京易醒生物科技有限公司 一种提高用于乙醇氧化的醇氧化酶表达量的方法、优化的核黄素生物合成基因

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0405370A1 (de) * 1989-06-22 1991-01-02 F. Hoffmann-La Roche Ag Riboflavin À¼berproduzierende Bakterienstämme
WO1993004180A1 (de) * 1991-08-22 1993-03-04 Basf Aktiengesellschaft Verfahren zur gezielten genetischen veränderung von ashbya gossypii
EP0569806A2 (de) * 1992-05-11 1993-11-18 BASF Aktiengesellschaft DNA-Verbindungen und rekombinante, die Ribloflavinsynthetaseaktivität von S. cerevisiae codierende DNA-Expressionsvektoren
DE4420785A1 (de) * 1994-03-25 1995-10-05 Basf Ag Riboflavin-Biosynthese in Pilzen
WO1997003208A1 (de) * 1995-07-13 1997-01-30 Basf Aktiengesellschaft Verfahren zur herstellung von riboflavin mittels mikroorganismen mit veränderter isocitratlyase-aktivität
EP0821063A2 (de) * 1996-07-24 1998-01-28 F. Hoffmann-La Roche Ag Verbesserte Riboflavin-Herstellung

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0405370A1 (de) * 1989-06-22 1991-01-02 F. Hoffmann-La Roche Ag Riboflavin À¼berproduzierende Bakterienstämme
WO1993004180A1 (de) * 1991-08-22 1993-03-04 Basf Aktiengesellschaft Verfahren zur gezielten genetischen veränderung von ashbya gossypii
EP0569806A2 (de) * 1992-05-11 1993-11-18 BASF Aktiengesellschaft DNA-Verbindungen und rekombinante, die Ribloflavinsynthetaseaktivität von S. cerevisiae codierende DNA-Expressionsvektoren
DE4420785A1 (de) * 1994-03-25 1995-10-05 Basf Ag Riboflavin-Biosynthese in Pilzen
WO1997003208A1 (de) * 1995-07-13 1997-01-30 Basf Aktiengesellschaft Verfahren zur herstellung von riboflavin mittels mikroorganismen mit veränderter isocitratlyase-aktivität
EP0821063A2 (de) * 1996-07-24 1998-01-28 F. Hoffmann-La Roche Ag Verbesserte Riboflavin-Herstellung

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BROWN D H JR ET AL: "Stable transformation and regulated expression of an inducible reporter construct in Candida albicans using restriction enzyme - mediated integration.", MOLECULAR AND GENERAL GENETICS, (1996 APR 24) 251 (1) 75-80., XP002123511 *

Also Published As

Publication number Publication date
WO1999061623A2 (de) 1999-12-02
EP1082438A2 (de) 2001-03-14
ID27073A (id) 2001-02-22
DE19823834A1 (de) 1999-12-02
RU2000133310A (ru) 2002-12-27
CN1303434A (zh) 2001-07-11
AU4140999A (en) 1999-12-13
JP2002516108A (ja) 2002-06-04
KR20010043867A (ko) 2001-05-25

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