WO1999036432A3 - Orotidin-5'-phosphatdecarboxylase-gen, genkonstrukt enthaltend dieses gen und seine verwendung - Google Patents

Orotidin-5'-phosphatdecarboxylase-gen, genkonstrukt enthaltend dieses gen und seine verwendung Download PDF

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Publication number
WO1999036432A3
WO1999036432A3 PCT/EP1998/008382 EP9808382W WO9936432A3 WO 1999036432 A3 WO1999036432 A3 WO 1999036432A3 EP 9808382 W EP9808382 W EP 9808382W WO 9936432 A3 WO9936432 A3 WO 9936432A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
orotidine
utilization
phosphate decarboxylase
construct containing
Prior art date
Application number
PCT/EP1998/008382
Other languages
English (en)
French (fr)
Other versions
WO1999036432A2 (de
Inventor
Markus Pompejus
Doval Jose Luis Revuelta
Garcia Maria Angeles Santos
Original Assignee
Basf Ag
Markus Pompejus
Doval Jose Luis Revuelta
Garcia Maria Angeles Santos
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag, Markus Pompejus, Doval Jose Luis Revuelta, Garcia Maria Angeles Santos filed Critical Basf Ag
Priority to EP98966388A priority Critical patent/EP1047710B1/de
Priority to JP2000540147A priority patent/JP2002508950A/ja
Priority to IL13700698A priority patent/IL137006A0/xx
Priority to DE59812837T priority patent/DE59812837D1/de
Priority to US09/582,779 priority patent/US6927026B1/en
Priority to AT98966388T priority patent/ATE296835T1/de
Publication of WO1999036432A2 publication Critical patent/WO1999036432A2/de
Publication of WO1999036432A3 publication Critical patent/WO1999036432A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01023—Orotidine-5'-phosphate decarboxylase (4.1.1.23)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein Orotidin-5'-Phosphatdecarboxylase-Gen mit der Sequenz SEQ ID No. 1 oder seine Homologen, ein Genkonstrukt enthaltend dieses Gen oder seine Homologen und dessen Verwendung. Die Erfindung betrifft ausserdem Vektoren oder Organismen enthaltend ein Orotidin-5'-Phosphatdecarboxylase-Gen mit der Sequenz SEQ ID No. 1 oder seine Homologen. Weiterhin betrifft die Erfindung ein Verfahren zur Herstellung von Uracil auxotrophen Mikroorganismen sowie ein Verfahren zum Einbringen von DNA in Uracil auxotrophe Mikroorganismen.
PCT/EP1998/008382 1998-01-15 1998-12-18 Orotidin-5'-phosphatdecarboxylase-gen, genkonstrukt enthaltend dieses gen und seine verwendung WO1999036432A2 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP98966388A EP1047710B1 (de) 1998-01-15 1998-12-18 Orotidin-5'-phosphatdecarboxylase-gen, genkonstrukt enthaltend dieses gen und seine verwendung
JP2000540147A JP2002508950A (ja) 1998-01-15 1998-12-18 オロチジン−５’−ホスフェートデカルボキシラーゼ−遺伝子、前記遺伝子を含有する遺伝子構築物およびその使用
IL13700698A IL137006A0 (en) 1998-01-15 1998-12-18 Orotidine 5'-phosphate decarboxylase-gene, gene construct containing said gene and the utilization thereof
DE59812837T DE59812837D1 (de) 1998-01-15 1998-12-18 Orotidin-5'-phosphatdecarboxylase-gen, genkonstrukt enthaltend dieses gen und seine verwendung
US09/582,779 US6927026B1 (en) 1998-01-15 1998-12-18 Orotidine-5′-phosphate decarboxylase-gene, gene construct containing said gene and the utilization thereof
AT98966388T ATE296835T1 (de) 1998-01-15 1998-12-18 Orotidin-5'-phosphatdecarboxylase-gen, genkonstrukt enthaltend dieses gen und seine verwendung

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19801120.2 1998-01-15
DE19801120A DE19801120A1 (de) 1998-01-15 1998-01-15 Orotidin-5'-Phosphatdecarboxylase-Gen, Genkonstrukt enthaltend dieses Gen und seine Verwendung

Publications (2)

Publication Number Publication Date
WO1999036432A2 WO1999036432A2 (de) 1999-07-22
WO1999036432A3 true WO1999036432A3 (de) 1999-08-19

Family

ID=7854573

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/008382 WO1999036432A2 (de) 1998-01-15 1998-12-18 Orotidin-5'-phosphatdecarboxylase-gen, genkonstrukt enthaltend dieses gen und seine verwendung

Country Status (9)

Country Link
US (1) US6927026B1 (de)
EP (1) EP1047710B1 (de)
JP (1) JP2002508950A (de)
CN (1) CN1161471C (de)
AT (1) ATE296835T1 (de)
DE (2) DE19801120A1 (de)
IL (1) IL137006A0 (de)
WO (1) WO1999036432A2 (de)
ZA (1) ZA99236B (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE481493T1 (de) * 2003-06-02 2010-10-15 B R A I N Biotechnology Res An Archäen-expressionssystem
WO2005052151A1 (en) * 2003-11-19 2005-06-09 Dow Global Technologies Inc. Improved protein expression systems
CN101321775B (zh) * 2005-10-03 2012-05-23 大学健康网络 用于治疗疟疾的odcase抑制剂
GB2433260A (en) * 2005-12-16 2007-06-20 Mologic Ltd A selectable decarboxylase marker
JP6092501B2 (ja) * 2011-06-02 2017-03-08 三井造船株式会社 ウラシル要求性モーレラ属細菌及び形質転換遺伝子導入モーレラ属細菌
BR112015014556A2 (pt) 2012-12-20 2017-10-10 Dsm Ip Assets Bv caroteno hidroxilase e seu uso para produzir carotenoides
BR112015014258B1 (pt) 2012-12-20 2022-05-17 Dsm Ip Assets B.V. Microrganismo transformado, seu processo de produção e uso para produzir carotenoides
CN112391329B (zh) * 2020-11-12 2023-07-25 江南大学 一种抗酸胁迫能力提高的大肠杆菌工程菌及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0011562A1 (de) * 1978-11-14 1980-05-28 ANVAR Agence Nationale de Valorisation de la Recherche Mit einem Plasmid transformierte Saccharomyces cer.
DE4420785A1 (de) * 1994-03-25 1995-10-05 Basf Ag Riboflavin-Biosynthese in Pilzen
WO1997003208A1 (de) * 1995-07-13 1997-01-30 Basf Aktiengesellschaft Verfahren zur herstellung von riboflavin mittels mikroorganismen mit veränderter isocitratlyase-aktivität

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4020181A1 (de) 1990-06-25 1992-01-02 Basf Ag Neue promotorregion
CA2186403C (en) 1994-03-25 2006-01-24 Jose Luis Revuelta Doval Riboflavin biosynthesis in fungi

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0011562A1 (de) * 1978-11-14 1980-05-28 ANVAR Agence Nationale de Valorisation de la Recherche Mit einem Plasmid transformierte Saccharomyces cer.
DE4420785A1 (de) * 1994-03-25 1995-10-05 Basf Ag Riboflavin-Biosynthese in Pilzen
WO1997003208A1 (de) * 1995-07-13 1997-01-30 Basf Aktiengesellschaft Verfahren zur herstellung von riboflavin mittels mikroorganismen mit veränderter isocitratlyase-aktivität

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ALTSCHUL S F ET AL: "BASIC LOCAL ALIGNMENT SEARCH TOOL", JOURNAL OF MOLECULAR BIOLOGY, vol. 215, 5 October 1990 (1990-10-05), pages 403 - 410, XP000604562 *
BENITO ERNESTO P. ET AL.,: "Isolation, characterization and transformation, by autonomous replication, of Mucor circinelloides OMPdecase-deficient mutants.", MOLECULAR & GENERAL GENETICS, vol. 248 (2), 1995, pages 126-135, XP002107255 *
D'ENFERT C.: "Selection of multiple disruption events in Aspergillus fumigatus using the orotidine-5'-decarboxylase gene, pyrG, as a unique transformation marker.", CURRENT GENETICS, vol. 30 (1), 1996, pages 76-82, XP002107254 *
M ROSE ET AL: "Structure and function of the yeast URA3 gene: expression in Escherichia coli", GENE, vol. 29, 1 January 1984 (1984-01-01), pages 113 - 124, XP002092104 *
P. ZHOU ET AL.,: "A system for gene cloning and manipulation in the yeast Candida glabrata", GENE, vol. 142, 1994, pages 135 - 140, XP002106569 *
R.M.J. BERGKAMP ET AL.,: "Cloning and sequencing of the URA3 gene of Kluyveromyces marxianus CBS 6556", YEAST, vol. 9, no. 6, 1993, pages 677 - 681, XP002107257 *
S. PIREDDA AND C. GALLARDIN: "Development of a transformation system for the yeast Yamadazyma (Pichia) ohmeri", YEAST, vol. 10, no. 12, 1994, pages 1601 - 1612, XP002107256 *

Also Published As

Publication number Publication date
IL137006A0 (en) 2001-06-14
DE19801120A1 (de) 1999-07-22
WO1999036432A2 (de) 1999-07-22
US6927026B1 (en) 2005-08-09
EP1047710B1 (de) 2005-06-01
CN1161471C (zh) 2004-08-11
ATE296835T1 (de) 2005-06-15
ZA99236B (en) 2000-07-14
JP2002508950A (ja) 2002-03-26
DE59812837D1 (de) 2005-07-07
EP1047710A2 (de) 2000-11-02
CN1285873A (zh) 2001-02-28

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