AU1491499A - Cloning method by multiple digestion, vectors for implementing same and applications - Google Patents

Cloning method by multiple digestion, vectors for implementing same and applications

Info

Publication number
AU1491499A
AU1491499A AU14914/99A AU1491499A AU1491499A AU 1491499 A AU1491499 A AU 1491499A AU 14914/99 A AU14914/99 A AU 14914/99A AU 1491499 A AU1491499 A AU 1491499A AU 1491499 A AU1491499 A AU 1491499A
Authority
AU
Australia
Prior art keywords
applications
vectors
cloning method
implementing same
multiple digestion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU14914/99A
Inventor
Marc Delcourt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomethodes SA
Original Assignee
Biomethodes SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomethodes SA filed Critical Biomethodes SA
Publication of AU1491499A publication Critical patent/AU1491499A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Electrotherapy Devices (AREA)
  • Debugging And Monitoring (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Measurement Of Velocity Or Position Using Acoustic Or Ultrasonic Waves (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Treatment Of Sludge (AREA)

Abstract

The invention concerns a novel cloning method based on the systematic, combined and preparatory use of restriction sites present on the inserts constituting a complementary or genome DNA bank. The invention also concerns the applications of said method and the resulting banks.
AU14914/99A 1997-12-04 1998-12-04 Cloning method by multiple digestion, vectors for implementing same and applications Abandoned AU1491499A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9715319 1997-12-04
FR9715319A FR2772048B1 (en) 1997-12-04 1997-12-04 MULTIPLE DIGESTION CLONING METHOD, VECTORS FOR IMPLEMENTING SAME AND APPLICATIONS THEREOF
PCT/FR1998/002629 WO1999028451A1 (en) 1997-12-04 1998-12-04 Cloning method by multiple digestion, vectors for implementing same and applications

Publications (1)

Publication Number Publication Date
AU1491499A true AU1491499A (en) 1999-06-16

Family

ID=9514169

Family Applications (1)

Application Number Title Priority Date Filing Date
AU14914/99A Abandoned AU1491499A (en) 1997-12-04 1998-12-04 Cloning method by multiple digestion, vectors for implementing same and applications

Country Status (8)

Country Link
EP (1) EP1034259B1 (en)
JP (1) JP2001525169A (en)
AT (1) ATE325189T1 (en)
AU (1) AU1491499A (en)
CA (1) CA2312029A1 (en)
DE (1) DE69834422T2 (en)
FR (1) FR2772048B1 (en)
WO (1) WO1999028451A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2789696A1 (en) * 1999-02-12 2000-08-18 Biomethodes Directed mutagenesis of nucleic acid useful e.g. for codon optimization, comprises polymerization-ligation reaction of cloned fragment in presence of mutagenic oligonucleotide
US7582423B2 (en) 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
JP4689940B2 (en) 2001-02-02 2011-06-01 ノビシ バイオテック，リミティド ライアビリティ カンパニー Method for increasing heteroduplex complementarity
US7838219B2 (en) 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
US7078211B2 (en) 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2042808C (en) * 1990-05-17 2000-04-25 Toshihiko Kishimoto Method of extracting particular nucleic acid fragment
US5500356A (en) * 1993-08-10 1996-03-19 Life Technologies, Inc. Method of nucleic acid sequence selection
FR2757537B1 (en) * 1996-12-23 1999-03-19 Genset Sa PROCESS FOR THE ELIMINATION OF SPECIFIC SEQUENCES DURING THE PRODUCTION OF DNA BANKS

Also Published As

Publication number Publication date
FR2772048A1 (en) 1999-06-11
WO1999028451A1 (en) 1999-06-10
ATE325189T1 (en) 2006-06-15
EP1034259B1 (en) 2006-05-03
DE69834422T2 (en) 2007-04-19
DE69834422D1 (en) 2006-06-08
EP1034259A1 (en) 2000-09-13
FR2772048B1 (en) 2001-12-21
CA2312029A1 (en) 1999-06-10
JP2001525169A (en) 2001-12-11

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase