WO1999058713A3 - Verfahren zur detektion von mikroorganismen in produkten - Google Patents

Verfahren zur detektion von mikroorganismen in produkten Download PDF

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Publication number
WO1999058713A3
WO1999058713A3 PCT/DE1999/001471 DE9901471W WO9958713A3 WO 1999058713 A3 WO1999058713 A3 WO 1999058713A3 DE 9901471 W DE9901471 W DE 9901471W WO 9958713 A3 WO9958713 A3 WO 9958713A3
Authority
WO
WIPO (PCT)
Prior art keywords
products
detecting microorganisms
detection
germs
pharmaceutical
Prior art date
Application number
PCT/DE1999/001471
Other languages
English (en)
French (fr)
Other versions
WO1999058713A2 (de
Inventor
Klaus-Peter Gerbling
Frank-Roman Lauter
Lutz Grohmann
Original Assignee
Bioinside Gmbh
Gerbling Klaus Peter
Lauter Frank Roman
Lutz Grohmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioinside Gmbh, Gerbling Klaus Peter, Lauter Frank Roman, Lutz Grohmann filed Critical Bioinside Gmbh
Priority to AU50260/99A priority Critical patent/AU5026099A/en
Priority to EP99934505A priority patent/EP1082465A2/de
Priority to DE19980848T priority patent/DE19980848D2/de
Priority to JP2000548504A priority patent/JP2002514439A/ja
Publication of WO1999058713A2 publication Critical patent/WO1999058713A2/de
Publication of WO1999058713A3 publication Critical patent/WO1999058713A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

Die Erfindung betrifft ein Detektionsverfahren und ein Testkit zur schnellen, ökonomischen Detektion von Keimen in pharmazeutischen und kosmetischen Produkten. Dabei werden spezifische Sonden und Primer eingesetzt, deren Replikation durch ein spezielles Indikatorsystem sichtbar gemacht wird, wobei ein Fluoreszenz-Farbstoff freigesetzt wird.
PCT/DE1999/001471 1998-05-12 1999-05-10 Verfahren zur detektion von mikroorganismen in produkten WO1999058713A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU50260/99A AU5026099A (en) 1998-05-12 1999-05-10 Method for detecting microorganisms in products
EP99934505A EP1082465A2 (de) 1998-05-12 1999-05-10 Verfahren zur detektion von mikroorganismen in produkten
DE19980848T DE19980848D2 (de) 1998-05-12 1999-05-10 Verfahren zur Detektion von Mikroorganismen in Produkten
JP2000548504A JP2002514439A (ja) 1998-05-12 1999-05-10 製品中の微生物の検出方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19822108.8 1998-05-12
DE19822108A DE19822108A1 (de) 1998-05-12 1998-05-12 Verfahren zur Detektion von Mikroorganismen in Produkten, insbesondere in Arzneimitteln und Kosmetika

Publications (2)

Publication Number Publication Date
WO1999058713A2 WO1999058713A2 (de) 1999-11-18
WO1999058713A3 true WO1999058713A3 (de) 2000-08-10

Family

ID=7868061

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/001471 WO1999058713A2 (de) 1998-05-12 1999-05-10 Verfahren zur detektion von mikroorganismen in produkten

Country Status (5)

Country Link
EP (1) EP1082465A2 (de)
JP (1) JP2002514439A (de)
AU (1) AU5026099A (de)
DE (2) DE19822108A1 (de)
WO (1) WO1999058713A2 (de)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU5267800A (en) 1999-05-03 2000-11-17 Gen-Probe Incorporated Polynucleotide probes for detection and quantitation of bacteria in the family enterobacteriaceae
US6821770B1 (en) 1999-05-03 2004-11-23 Gen-Probe Incorporated Polynucleotide matrix-based method of identifying microorganisms
DE10004147A1 (de) * 2000-01-31 2001-08-09 Gsf Forschungszentrum Umwelt Oligonukleotide zur spezifischen Amplifikation und zum spezifischen Nachweis von 16S-rRNA-Genen von Bakterien
DE10010614A1 (de) * 2000-03-03 2001-09-13 Gsf Forschungszentrum Umwelt Oligonukleotidsonden zum art- und/oder gattungsspezifischen Nachweis von das Pflanzenwachstum fördernden Bakterien
FR2811321A1 (fr) * 2000-07-04 2002-01-11 Bio Merieux Amplificateur d'une region ribonucleique cible d'un arn ribosomal 16s ou adn pour un tel arn d'une espece eubacterienne et detection de telles especes
AUPQ909000A0 (en) * 2000-07-28 2000-08-24 University Of Sydney, The A method of detecting microorganisms
US6893847B2 (en) * 2001-01-17 2005-05-17 Tosoh Corporation Oligonucleotide for detecting Salmonella and method of detecting Salmonella
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US20040121309A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
AT411174B (de) * 2001-08-09 2003-10-27 Lambda Labor Fuer Molekularbio Verfahren und chip zur analyse von nukleinsäuren
AU2003288018A1 (en) * 2002-11-20 2004-06-15 Pharmacia Italia S.P.A. Method for the detection of microorganisms in pharmaceutical products
JP2006516193A (ja) 2002-12-06 2006-06-29 アイシス・ファーマシューティカルス・インコーポレーテッド ヒトおよび動物における病原体の迅速な同定方法
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8394945B2 (en) 2003-09-11 2013-03-12 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8163895B2 (en) 2003-12-05 2012-04-24 Ibis Biosciences, Inc. Compositions for use in identification of orthopoxviruses
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
CA2567839C (en) 2004-05-24 2011-06-28 Isis Pharmaceuticals, Inc. Mass spectrometry with selective ion filtration by digital thresholding
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
CA2600184A1 (en) 2005-03-03 2006-09-08 Isis Pharmaceuticals, Inc. Compositions for use in identification of adventitious viruses
US20110159488A1 (en) 2005-04-01 2011-06-30 Smartgene Gmbh Primers for Amplification and Sequencing of Eubacterial 16S rDNA for Identification
CA2616281C (en) 2005-07-21 2014-04-22 Isis Pharmaceuticals, Inc. Methods for rapid identification and quantitation of mitochondrial dna variants
EP2064332B1 (de) 2006-09-14 2012-07-18 Ibis Biosciences, Inc. Gezielte gesamtgenomamplifizierung zur identifizierung von krankheitserregern
WO2008104002A2 (en) 2007-02-23 2008-08-28 Ibis Biosciences, Inc. Methods for rapid forensic dna analysis
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
US8148163B2 (en) 2008-09-16 2012-04-03 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
WO2010033625A1 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
US8550694B2 (en) 2008-09-16 2013-10-08 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
WO2010093943A1 (en) 2009-02-12 2010-08-19 Ibis Biosciences, Inc. Ionization probe assemblies
EP2454000A4 (de) 2009-07-17 2016-08-10 Ibis Biosciences Inc Systeme zur identifizierung von biowirkstoffen
US8950604B2 (en) 2009-07-17 2015-02-10 Ibis Biosciences, Inc. Lift and mount apparatus
EP2488656B1 (de) 2009-10-15 2015-06-03 Ibis Biosciences, Inc. Mehrfache verschiebungsverstärkung
US9034581B2 (en) * 2011-05-26 2015-05-19 Roche Molecular Systems, Inc. Compositions and methods for detection of Staphylococcus aureus
WO2016012508A1 (en) * 2014-07-23 2016-01-28 Steffen Mergemeier Method for the detection of sepsis
WO2024077197A1 (en) * 2022-10-05 2024-04-11 Life Technologies Corporation Multiplex qpcr panel for gastrointestinal pathogens

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015157A1 (en) * 1989-05-31 1990-12-13 Gene-Trak Systems Universal eubacteria nucleic acid probes and methods
WO1991008305A1 (en) * 1989-11-27 1991-06-13 U-Gene Research B.V. Dna fragments and dna probes and primers based thereon
WO1992002638A1 (en) * 1990-08-06 1992-02-20 F. Hoffmann-La Roche Ag Homogeneous assay system
WO1993003186A1 (en) * 1991-07-31 1993-02-18 F.Hoffmann-La Roche Ag Methods and reagents for detection of bacteria in cerebrospinal fluid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015157A1 (en) * 1989-05-31 1990-12-13 Gene-Trak Systems Universal eubacteria nucleic acid probes and methods
WO1991008305A1 (en) * 1989-11-27 1991-06-13 U-Gene Research B.V. Dna fragments and dna probes and primers based thereon
WO1992002638A1 (en) * 1990-08-06 1992-02-20 F. Hoffmann-La Roche Ag Homogeneous assay system
WO1993003186A1 (en) * 1991-07-31 1993-02-18 F.Hoffmann-La Roche Ag Methods and reagents for detection of bacteria in cerebrospinal fluid

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LEE L G ET AL: "ALLELIC DISCRIMINATION BY NICK-TRANSLATION PCR WITH FLUOROGENIC PROBES", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 21, no. 16, 11 August 1993 (1993-08-11), pages 3761 - 3766, XP000470188, ISSN: 0305-1048 *
S. SAU ET AL.: "Cloning of type 8 capsule genes and analysis of gene clusters for the production of different capsular polysaccharides in Staphylococcus aureus", JOURNAL OF BACTERIOLOGY, vol. 178, no. 7, April 1996 (1996-04-01), US, pages 2118 - 2126, XP000872772 *

Also Published As

Publication number Publication date
AU5026099A (en) 1999-11-29
DE19822108A1 (de) 2000-02-03
JP2002514439A (ja) 2002-05-21
DE19980848D2 (de) 2001-06-13
WO1999058713A2 (de) 1999-11-18
EP1082465A2 (de) 2001-03-14

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