WO1999056104A1 - Dispositif permettant d'enrichir des particules contenues dans les liquides du corps - Google Patents

Dispositif permettant d'enrichir des particules contenues dans les liquides du corps Download PDF

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Publication number
WO1999056104A1
WO1999056104A1 PCT/DE1998/001988 DE9801988W WO9956104A1 WO 1999056104 A1 WO1999056104 A1 WO 1999056104A1 DE 9801988 W DE9801988 W DE 9801988W WO 9956104 A1 WO9956104 A1 WO 9956104A1
Authority
WO
WIPO (PCT)
Prior art keywords
receiving body
filter
filter insert
piston
pipette tip
Prior art date
Application number
PCT/DE1998/001988
Other languages
German (de)
English (en)
Inventor
Rainer Ballnath
Helmut Fromm
Original Assignee
Rainer Ballnath
Helmut Fromm
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rainer Ballnath, Helmut Fromm filed Critical Rainer Ballnath
Priority to JP2000546216A priority Critical patent/JP2002513150A/ja
Priority to EP98944993A priority patent/EP1075649A1/fr
Priority to AU92525/98A priority patent/AU9252598A/en
Publication of WO1999056104A1 publication Critical patent/WO1999056104A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/493—Physical analysis of biological material of liquid biological material urine

Definitions

  • the present invention relates to a device for enriching particles in body fluid, in particular for providing an enriched suspension for analysis, with a preferably cylindrical receiving body and a piston which is displaceably arranged in the receiving body and is used for drawing in and expelling the liquid, the receiving body being a pipette-like has tapered suction / outlet opening.
  • a device for urine examination is known, specifically a device for microscopic quantitative urine sediment analysis.
  • This device comprises a cylindrical receiving body or sample container which has an opening on one side and a pipette tip on the other side.
  • a sample container is inserted into the receiving body.
  • a tubular piston in the receiving body which is displaceable in a sealing manner, both the piston and the sample container being made of a transparent material.
  • a stopper is used to close the sample container.
  • a cap for the pipette tip is also provided.
  • a one- or multi-stage filter arrangement is provided in the piston near the lower end, and is integrated in the piston.
  • the device known from DE 37 19 302 for the enrichment of particles in body fluid is extremely problematic to handle.
  • the piston must be closed with the plug.
  • the plunger is pulled out until the urine drawn in has reached a predetermined mark on the sample container with its liquid upper edge.
  • the tip of the sample container is closed with the cap.
  • the plug must be from the top the piston.
  • the piston is slowly and evenly fully inserted into the sample container. Afterwards, the sample container has to be inverted once with the piston inserted in order to pour off the excess urine, which is then discarded. The sample container is then reversed again.
  • the present invention is based on the object of designing and developing a generic device for enriching particles in body fluid in such a way that simple handling is possible without contaminating the operator with the body fluid.
  • a simple construction of the device should keep the costs as low as possible.
  • the present invention solves the above object by the features of claim 1. Thereafter, the generic device for enriching particles in body fluid is designed and developed in such a way that in the flow path of the liquid flowing into and / or out of the receiving body, a fluid trapping the particles or receiving catcher is arranged.
  • a special collecting device for floating particles in the body fluid is provided here, so that the collecting device serves on the one hand to intercept and on the other hand to receive and store or store the floating particles or particles in the body liquid.
  • the collecting device could be assigned to the inlet-side end of the receiving body, so that the collecting device is arranged between the piston used for suction and the suction / outlet opening which tapers like a pipette.
  • the collecting device could also be assigned directly to the suction / outlet opening, namely it could be arranged within a component comprising the suction / outlet opening, so that a separation from the collecting device is ensured. In such a case, the collecting device could be easily removed from the receiving body.
  • the collecting device could be assigned to the piston surface, through openings being formed in the piston surface.
  • the inside of the piston would be designed as a collecting container, the inflow of liquid into the collecting container being controllable by preferably manually opening and closing a ventilation valve formed at the end in the piston. It is essential that the body fluid is first sucked into the receiving body and, when the suction / outlet opening is closed, by pressing the piston in the direction opposite to the suction movement, it is pressed into the inside of the piston or into the collecting container. The body fluid passes through the collecting device, so that a thickening of the liquid takes place in the area of the collecting device, ie in the area of the piston surface.
  • the collecting device could be designed as a filter, with different filters for different fractions being able to be used depending on the particles or particles to be filtered out.
  • the filter could be cellulose or a filter made of synthetic fibers or synthetic fabric. It is also conceivable to form the filter from an absorbent material with open porosity or passage porosity, so that passage of the liquid through the filter and sticking of the particles of interest in the filter is ensured. In any case, a "thickening" or an accumulation of the particles takes place within the filter.
  • the collecting device or the filter could be assigned to a filter insert and be removable from the filter insert.
  • the interchangeability of the filter or the removability of the filter insert is ensured, so that it is also easily possible to remove the filter containing the trapped particles together with the filter insert and to carry them out for further analysis or processing.
  • the filter insert could be inserted into the inlet-side end of the receiving body and hold the collecting device there. It is also conceivable that the filter insert is an integral part of the inlet end of the receiving body.
  • the filter insert together with the filter could be attached to the piston surface.
  • the filter insert could be an integral part of the piston surface.
  • the filter insert comprises a liquid-permeable bottom and a wall suitable for holding the filter.
  • the wall of the filter insert could encompass the filter as a whole and overlap, at least partially, at the edge area so that the filter is held securely in the filter insert.
  • the filter of the through the Piston generated flow withstand the liquid and must not change its position.
  • the filter insert is at least partially elastically deformable, and preferably compressible at least slightly in the axial direction. In the context of such a configuration, it is ensured that the filter and the filter insert can be virtually squeezed out, as a result of which the parts caught there are at least largely released for analysis or further processing.
  • the suction / outlet opening could be an integral part of the receiving body, in which case it would have the suction / outlet opening tapering like a pipette.
  • the suction outlet opening is formed by a pipette tip which can be plugged onto the receptacle body or inserted into the receptacle body. In both cases, the filter insert could be inserted into the pipette tip or into the corresponding component.
  • the collecting device or the filter can be removed by removing the filter insert, which ensures that the device can be handled particularly easily.
  • the filter insert is an integral part of the pipette tip, so that the filter is only clamped or inserted into the filter insert integrated there.
  • a pluggable or screw-on closure is provided for closing the pipette tip.
  • This closure is advantageously adapted essentially to the shape of the pipette tip, at least on the inside.
  • the closure has essentially the same cross-section as the receiving body, so that when the pipette tip is closed, the device has a homogeneous appearance, which ensures particularly simple and safe handling, storage, stackability, etc.
  • the individual components can be made entirely or at least predominantly of plastic, at least the receiving body being made of a transparent material. An observation of the sucked-in liquid but above all of the filter used there is guaranteed.
  • FIG. 1 shows a schematic side view, in section, of a first exemplary embodiment of a device according to the invention, the collecting device being assigned to the inlet-side end of the receiving body there,
  • FIG. 2 shows a schematic side view, in section, of a second exemplary embodiment of a device according to the invention, the suction / outlet opening which tapers like a pipette being designed in the form of a screw-on pipette tip,
  • FIG. 3 shows a schematic side view, in section, of a third exemplary embodiment, the collecting device being assigned to the piston surface there,
  • FIG. 4 in a schematic view, enlarged, a filter insert with an inserted filter, as it is used in the embodiments according to FIGS. 1 to 3 and 7
  • Fig. 5 in a schematic side view, sectioned and enlarged, a plug-on closure on the pipette tip.
  • FIGS. 1, 2 and 3 show mutually independent embodiments of a device according to the invention for the enrichment of particles in body fluid, this specifically being a device for providing an enriched suspension for analysis, namely for urinalysis.
  • An examination of the "thickened" urine should thus be possible in the simplest possible way, in order to be able to diagnose inflammation or other diseases due to constituents in the urine. Normal centrifugation of the liquid is not necessary due to the thickening achieved.
  • the device comprises a cylindrical receptacle body 1 and a piston 2 which is displaceably arranged in the receptacle body 1 and is used for drawing in and expelling the liquid.
  • the receptacle body 1 has a suction / outlet opening 3 which tapers like a pipette.
  • a collecting device 4 which catches or receives the particles is arranged in the flow path of the liquid flowing into and / or flowing out of the receiving body 1.
  • the collecting device is assigned to the inlet-side end of the receiving body 1.
  • the collecting device 1 is likewise assigned to the inlet-side end of the receiving body 1, the collecting device 4 being able to be assigned to the suction / outlet opening 3 or the component comprising the suction / outlet opening.
  • the collecting device 4 is assigned to the piston surface 5, the piston surface 5 including through openings 6.
  • further sealing means 7 are provided in order to effectively prevent the liquid from escaping to the rear end of the receiving body 1. 8th
  • the interior of the piston is designed as a collecting container 8, in order to be able to receive the liquid through the collecting device 4 inside the piston 2.
  • Fig. 3 further shows that the inflow of liquid into the collecting container 8 by manual opening and closing of a ventilation valve 9 formed at the end in the piston 2 can be regulated, wherein this ventilation valve 9 can be revealed and closed by a stopper or by finger pressure.
  • the collecting device 4 is designed as a filter, the filter being a loose cellulose fabric. Synthetic material can also be used, whereby the formation of a predeterminable fraction must be ensured.
  • FIGS. 1 and 2 show particularly clearly that the collecting device 4 is assigned to a filter insert 10.
  • the filter 11 can be removed from the filter insert 10.
  • the filter insert 10 can be installed in the receiving body 1 in different ways, namely as shown in FIGS. 1 and 2.
  • This filter insert 10 is also suitable for placement on the piston 2, so that a direct assignment of the filter insert 10 to the piston 2 is ensured.
  • the filter insert 10 comprises a liquid-permeable bottom area 13 and a wall 14 suitable for holding the filter 11. This wall 14 surrounds the filter 11 as a whole and partially overlaps the filter 11, so that a locking or holding the Filters 11 is guaranteed within the filter insert 10.
  • the pipette tip 15 is an integral part of the receiving body 1.
  • the pipette tip 15 is an independent component, which is screwed onto the receiving body 1 from the outside. This is only indicated in Fig. 2.
  • a pluggable closure 16 which is shown in isolation in FIG. 5, is provided for closing the pipette tip 15.
  • the interior of this closure 16 corresponds essentially to the shape of the pipette tip 15 or is adapted to the pipette tip 15.
  • the device discussed above with reference to three exemplary embodiments can be used to find erythrocytes and leukocytes in the urine, namely to compress these components (cells) in the urine.
  • the liquid - or, in the example discussed here, the urine - is drawn out of a sample container not shown in the figures into the receiving body 1, and in the case of the exemplary embodiments shown in FIGS. 1 and 2 by the collecting device 4 or the Filter 11 pulled through.
  • the passage through the collecting device 4 or the filter 11 only takes place when the liquid is ejected or when the liquid penetrates into the interior of the piston or into the collecting container 8.
  • the cells in the urine settle on or in the filter 11.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un dispositif permettant d'enrichir les particules contenues dans les liquides du corps, notamment pour préparer une suspension enrichie aux fins d'analyse. Ce dispositif comprend un corps récepteur (1) de préférence cylindrique et un piston (2) qui est placé mobile dans le corps récepteur (1) et qui sert à aspirer et à expulser le liquide. Le corps récepteur (1) présente un orifice d'aspiration/d'évacuation (3) effilé en forme de pipette. Pour faciliter la manipulation et simplifier la construction de ce dispositif et éviter toute contamination du personnel par les liquides du corps, ce dispositif est conçu et perfectionné de telle façon qu'un dispositif collecteur (4) piégeant ou recueillant les particules est placé sur le chemin d'écoulement des liquides du corps qui affluent dans le corps récepteur (1) et/ou sont évacués du corps récepteur (1).
PCT/DE1998/001988 1998-04-29 1998-07-16 Dispositif permettant d'enrichir des particules contenues dans les liquides du corps WO1999056104A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2000546216A JP2002513150A (ja) 1998-04-29 1998-07-16 体液内の粒子を濃縮するための装置
EP98944993A EP1075649A1 (fr) 1998-04-29 1998-07-16 Dispositif permettant d'enrichir des particules contenues dans les liquides du corps
AU92525/98A AU9252598A (en) 1998-04-29 1998-07-16 Device for enriching particles in a body fluid

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE1998120178 DE19820178C2 (de) 1998-04-29 1998-04-29 Vorrichtung zur Anreicherung von Teilchen in Körperflüssigkeit
DE19820178.8 1998-04-29

Publications (1)

Publication Number Publication Date
WO1999056104A1 true WO1999056104A1 (fr) 1999-11-04

Family

ID=7866819

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1998/001988 WO1999056104A1 (fr) 1998-04-29 1998-07-16 Dispositif permettant d'enrichir des particules contenues dans les liquides du corps

Country Status (5)

Country Link
EP (1) EP1075649A1 (fr)
JP (1) JP2002513150A (fr)
AU (1) AU9252598A (fr)
DE (1) DE19820178C2 (fr)
WO (1) WO1999056104A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003533689A (ja) * 2000-05-18 2003-11-11 シュリューター ゲルト 検査用注射器、特に体液から細胞材料を回収するための濾過装置を備えた検査用注射器

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4157400B2 (ja) * 2003-03-13 2008-10-01 株式会社トクヤマ 抗原抽出液の製造方法。
WO2008134423A1 (fr) * 2007-04-24 2008-11-06 Porex Corporation Pipette jetable, et ses procédés de fabrication et d'utilisation
FR2935392B1 (fr) * 2008-09-02 2014-09-05 Metagenex Dispositif et procede pour isoler et cultiver des cellules vivantes sur filtre ou extraire leur materiel genetique
JP5385304B2 (ja) * 2008-01-09 2014-01-08 スクリーンセル 生きた細胞をフィルタ上で分離して培養するまたはその細胞の遺伝子材料を抽出するための装置と方法
FR2934681B1 (fr) * 2008-07-29 2011-09-30 Bastien Karkouche Dispositif pour la capture de particules biologiques et utilisation.
WO2012001607A1 (fr) * 2010-06-29 2012-01-05 Yadav, Omprakash Trousse d'homogénéisateur à usage unique

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Publication number Priority date Publication date Assignee Title
DE3223589A1 (de) * 1982-06-24 1983-12-29 Bernhard Dr.med. 3004 Isernhagen Aeikens Verfahren und vorrichtung zur praeparation von fluessigkeitsproben wie urin oder anderen koerperfluessigkeiten fuer den transport an eine auswertestelle
DE3719302C1 (de) * 1987-06-10 1988-12-29 Hoyer Gmbh & Co Vorrichtung zur Harnuntersuchung
US4960130A (en) * 1989-01-10 1990-10-02 Cancer Diagnostics, Inc. Modular fluid sample preparation assembly
WO1992017110A1 (fr) * 1991-04-08 1992-10-15 La Mina Ltd. Procede et dispositif de cytologie et d'experimentation microbiologique

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US4267729A (en) * 1979-05-14 1981-05-19 Eddelman Roy T Volumetric pipettor
US4787971A (en) * 1987-01-23 1988-11-29 Alan Donald Miniaturized column chromatography separation apparatus and method of assaying biomolecules employing the same
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WO1989012229A1 (fr) * 1988-06-03 1989-12-14 Manac Inc. Colonne du type a seringue pour chromatographie a l'etat liquide
US5022411A (en) * 1989-09-18 1991-06-11 La Mina Ltd. Modular fluid testing device
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EP0688426A1 (fr) * 1993-03-08 1995-12-27 WAINWRIGHT, Norman Chromatographie a fibres alignees a usage diagnostique

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3223589A1 (de) * 1982-06-24 1983-12-29 Bernhard Dr.med. 3004 Isernhagen Aeikens Verfahren und vorrichtung zur praeparation von fluessigkeitsproben wie urin oder anderen koerperfluessigkeiten fuer den transport an eine auswertestelle
DE3719302C1 (de) * 1987-06-10 1988-12-29 Hoyer Gmbh & Co Vorrichtung zur Harnuntersuchung
US4960130A (en) * 1989-01-10 1990-10-02 Cancer Diagnostics, Inc. Modular fluid sample preparation assembly
WO1992017110A1 (fr) * 1991-04-08 1992-10-15 La Mina Ltd. Procede et dispositif de cytologie et d'experimentation microbiologique

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003533689A (ja) * 2000-05-18 2003-11-11 シュリューター ゲルト 検査用注射器、特に体液から細胞材料を回収するための濾過装置を備えた検査用注射器

Also Published As

Publication number Publication date
AU9252598A (en) 1999-11-16
DE19820178A1 (de) 1999-11-11
EP1075649A1 (fr) 2001-02-14
JP2002513150A (ja) 2002-05-08
DE19820178C2 (de) 2000-11-30

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