EP1682275A1 - Pipette a echantillons - Google Patents
Pipette a echantillonsInfo
- Publication number
- EP1682275A1 EP1682275A1 EP04789822A EP04789822A EP1682275A1 EP 1682275 A1 EP1682275 A1 EP 1682275A1 EP 04789822 A EP04789822 A EP 04789822A EP 04789822 A EP04789822 A EP 04789822A EP 1682275 A1 EP1682275 A1 EP 1682275A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- sample
- cannula
- functional element
- hollow needle
- sample pipette
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0275—Interchangeable or disposable dispensing tips
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/16—Injection
- G01N30/18—Injection using a septum or microsyringe
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
- B01L2300/0838—Capillaries
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/022—Capillary pipettes, i.e. having very small bore
Definitions
- the invention relates to a sample pipette with a hollow needle for drawing up a volume of liquid, for example a sample, a reagent, etc., and for ejecting it into a vessel or into a sample receiving inlet of an analysis device, in particular for sample injection into an HPLC device.
- Sample preparation is often required between taking a sample and entering it into a chromatography device.
- the sample is ejected from the removal pipette into a processing vessel and, after processing, drawn up into a sample delivery pipette.
- a simplification is achieved by an interchangeable cannula fitted with functional elements, for example filters, coatings with active phases, etc., made of an injection needle and an attachment part, which is placed on the hollow needle.
- functional elements for example filters, coatings with active phases, etc.
- cannula In medicine and in related areas, the term cannula is usually one-sided for piercing suitable injection needle provided at the other end with a plug-in cone for connection to a syringe etc.
- the term is used in a similar manner in the present description, with a special attachment part being present instead of the plug-in cone.
- injection needle is used for that of the cannula.
- Fig. 1 shows a sample pipette with a replaceable cannula attached.
- Fig. 2 is a sectional view of the assembled cannula
- Fig. 3 is a sectional view of the exploded individual parts of the cannula
- the pipette shows a sample application pipette in the form of a syringe for manual sample application in an HPLC device.
- the pipette consists essentially of a cylinder 1, at one end 2 of which a hollow needle 4 connected to its interior 3 is arranged, and a piston 5 which is axially movable in the interior and is actuated from the other end 6 of the cylinder.
- a hollow needle 4 connected to its interior 3
- piston 5 which is axially movable in the interior and is actuated from the other end 6 of the cylinder.
- a cannula 7 is arranged on the free end of the hollow needle 4, which is merely plugged on and therefore easily is changeable.
- the cannula 7 is designed for single use, ie a new cannula is generally used for each new proloe. For certain work steps it may make sense to change the cannula even between drawing up and ejecting a sample, as described below.
- the cannula 7 shown in the sectional views in FIGS. 2 and 3 consists of an injection needle 8 and an attachment part 9.
- the attachment part 9 consists of a receiving sleeve 10, an insertion sleeve 11 arranged coaxially therein and a functional element 12 arranged in between.
- the receiving sleeve 10 is firmly connected on one side to the injection needle 8. From its other side, a weakly conical bore 13 extends to the injection needle 8.
- the receiving sleeve 10 essentially has the shape of the plug-in cone of the Luer type known from commercially available cannulas.
- the insertion sleeve 11 has a conical outer wall 14 corresponding to the bore 13 and a continuous axial channel 15.
- the channel 15 is widened at its end facing the receiving sleeve to a cylindrical chamber 16 which serves to receive the functional element 12.
- a funnel-shaped extension 17 is formed, by means of which the insertion of the hollow needle 4 is facilitated when the cannula is replaced.
- the central part 18 of the channel 15 is weakly conical or cylindrical and so narrow that it tightly encloses the hollow needle of the syringe.
- a constriction 19 is provided in front of the chamber to limit the depth of insertion of the hollow needle.
- the closed sides of the chamber 16 and the bore 13 are recessed conically. This results in 12 flat, free cavities on both sides of the functional element used.
- the functional element 12 is a filter, for example. This is used to filter a sample when it is drawn up in order to separate undesired components of the sample. In order to avoid entrainment of the filtered components during the subsequent injection of such a filtered sample into an analysis device, the cannula used for drawing up is expediently removed or replaced by a new cannula with or without a filter.
- the flat cavities on both sides of the functional element 12 achieve a uniform distribution of the sample over the entire active cross section.
- the functional element 12 can also be a package with an active phase, on which components of the sample are adsorbed. These components can be redissolved in a subsequent step using a suitable solvent. As with filtering, these steps are possible both when opening and when ejecting.
- a pipette provided with this cannula can therefore be used very advantageously for sample preparation.
- Samples which are taken up and ejected treated by one or two different cannulas are dispensed into a vessel, for example a cuvette of a microtiter plate, and further processed there by mixing etc. before they are entered into an analysis device.
- sample pipette according to the invention is evident in the fact that the liquid volume after being drawn up in the hollow needle is on the other side of the cannula. This makes it possible to exchange the cannula for another cannula before ejecting it.
- the attachment part can also take forms other than those shown in the present exemplary embodiment.
- cannulas can also be provided, the attachment part of which has a larger internal volume.
- a liquid volume can only be drawn into the cannula. This can be expedient, for example, if there is already a sample inside the hollow needle and a mixture is to take place when it is ejected.
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Pipettes à échantillons utilisées en particulier pour l'injection d'échantillons dans des appareils de chromatographie liquide à haute pression, qui sont pourvues d'une aiguille creuse (4) destinée à prélever un échantillon et à expulser ce dernier dans un récipient ou dans le dispositif récepteur d'échantillon de l'appareil. Une canule interchangeable (7) est montée sur l'aiguille creuse d'une pipette de ce type, ladite canule pouvant contenir des éléments fonctionnels supplémentaires (12) tels que des filtres, des phases chimiquement actives, etc.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CH18582003 | 2003-10-31 | ||
PCT/CH2004/000654 WO2005042166A1 (fr) | 2003-10-31 | 2004-10-29 | Pipette a echantillons |
Publications (1)
Publication Number | Publication Date |
---|---|
EP1682275A1 true EP1682275A1 (fr) | 2006-07-26 |
Family
ID=34529363
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP04789822A Withdrawn EP1682275A1 (fr) | 2003-10-31 | 2004-10-29 | Pipette a echantillons |
Country Status (3)
Country | Link |
---|---|
US (1) | US7434483B2 (fr) |
EP (1) | EP1682275A1 (fr) |
WO (1) | WO2005042166A1 (fr) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005057206A1 (fr) * | 2003-12-09 | 2005-06-23 | Bgb Analytik Ag | Dispositif pour preparer un echantillon |
US7798021B2 (en) | 2006-01-12 | 2010-09-21 | Gamble Kimberly R | Method and apparatus for sample processing and injection |
US8291778B2 (en) * | 2006-09-08 | 2012-10-23 | Ronald Rockwell Rich | Extractive sampling system for fluids |
CN101792105B (zh) * | 2009-02-04 | 2012-09-26 | 刘伟耀 | 定量取液器 |
US9891149B2 (en) | 2011-08-08 | 2018-02-13 | Thermo Fisher Scientific Oy | Method and apparatus for automated analysis |
FI20115785A0 (fi) * | 2011-08-08 | 2011-08-08 | Thermo Fisher Scientific Oy | Menetelmä ja laite automaattiseen analyysiin |
DE102012015063B4 (de) * | 2012-07-31 | 2014-10-30 | Sartorius Stedim Biotech Gmbh | Vorrichtung und Verfahren zur Behandlung eines Filtrationsmediums |
CN112206844A (zh) * | 2020-08-17 | 2021-01-12 | 武汉迈特维尔生物科技有限公司 | 一种过滤小体积生物样品溶液的装置及方法 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4001929A (en) * | 1975-07-30 | 1977-01-11 | Soji Ishikawa | Method of constructing a medical needle holder assembly |
US4316462A (en) | 1980-05-21 | 1982-02-23 | Siloam, Inc. | Filtering device for an injection device |
JPS5861251U (ja) | 1981-10-22 | 1983-04-25 | 石川 惣二 | 注射針 |
EP0201611A1 (fr) * | 1985-05-10 | 1986-11-20 | B. Braun-SSC AG | Seringue à deux canules |
EP1382963B1 (fr) * | 2001-04-20 | 2008-09-03 | Gl Sciences Incorporated | Procede et instrument d'extraction d'un composant a l'etat de trace en phase solide |
US20030087454A1 (en) | 2001-10-26 | 2003-05-08 | Schultz Gary A | Method and device for chemical analysis |
-
2004
- 2004-10-29 EP EP04789822A patent/EP1682275A1/fr not_active Withdrawn
- 2004-10-29 US US10/575,721 patent/US7434483B2/en not_active Expired - Fee Related
- 2004-10-29 WO PCT/CH2004/000654 patent/WO2005042166A1/fr active Application Filing
Non-Patent Citations (1)
Title |
---|
See references of WO2005042166A1 * |
Also Published As
Publication number | Publication date |
---|---|
US7434483B2 (en) | 2008-10-14 |
US20070056350A1 (en) | 2007-03-15 |
WO2005042166A1 (fr) | 2005-05-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
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17P | Request for examination filed |
Effective date: 20060531 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR |
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DAX | Request for extension of the european patent (deleted) | ||
17Q | First examination report despatched |
Effective date: 20120525 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20141025 |