EP1682275A1 - Sample pipette - Google Patents

Sample pipette

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Publication number
EP1682275A1
EP1682275A1 EP04789822A EP04789822A EP1682275A1 EP 1682275 A1 EP1682275 A1 EP 1682275A1 EP 04789822 A EP04789822 A EP 04789822A EP 04789822 A EP04789822 A EP 04789822A EP 1682275 A1 EP1682275 A1 EP 1682275A1
Authority
EP
European Patent Office
Prior art keywords
sample
cannula
functional element
hollow needle
sample pipette
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP04789822A
Other languages
German (de)
French (fr)
Inventor
Hansjörg Cueni
Heiner Scherrer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP1682275A1 publication Critical patent/EP1682275A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/16—Injection
    • G01N30/18—Injection using a septum or microsyringe
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/022—Capillary pipettes, i.e. having very small bore

Definitions

  • the invention relates to a sample pipette with a hollow needle for drawing up a volume of liquid, for example a sample, a reagent, etc., and for ejecting it into a vessel or into a sample receiving inlet of an analysis device, in particular for sample injection into an HPLC device.
  • Sample preparation is often required between taking a sample and entering it into a chromatography device.
  • the sample is ejected from the removal pipette into a processing vessel and, after processing, drawn up into a sample delivery pipette.
  • a simplification is achieved by an interchangeable cannula fitted with functional elements, for example filters, coatings with active phases, etc., made of an injection needle and an attachment part, which is placed on the hollow needle.
  • functional elements for example filters, coatings with active phases, etc.
  • cannula In medicine and in related areas, the term cannula is usually one-sided for piercing suitable injection needle provided at the other end with a plug-in cone for connection to a syringe etc.
  • the term is used in a similar manner in the present description, with a special attachment part being present instead of the plug-in cone.
  • injection needle is used for that of the cannula.
  • Fig. 1 shows a sample pipette with a replaceable cannula attached.
  • Fig. 2 is a sectional view of the assembled cannula
  • Fig. 3 is a sectional view of the exploded individual parts of the cannula
  • the pipette shows a sample application pipette in the form of a syringe for manual sample application in an HPLC device.
  • the pipette consists essentially of a cylinder 1, at one end 2 of which a hollow needle 4 connected to its interior 3 is arranged, and a piston 5 which is axially movable in the interior and is actuated from the other end 6 of the cylinder.
  • a hollow needle 4 connected to its interior 3
  • piston 5 which is axially movable in the interior and is actuated from the other end 6 of the cylinder.
  • a cannula 7 is arranged on the free end of the hollow needle 4, which is merely plugged on and therefore easily is changeable.
  • the cannula 7 is designed for single use, ie a new cannula is generally used for each new proloe. For certain work steps it may make sense to change the cannula even between drawing up and ejecting a sample, as described below.
  • the cannula 7 shown in the sectional views in FIGS. 2 and 3 consists of an injection needle 8 and an attachment part 9.
  • the attachment part 9 consists of a receiving sleeve 10, an insertion sleeve 11 arranged coaxially therein and a functional element 12 arranged in between.
  • the receiving sleeve 10 is firmly connected on one side to the injection needle 8. From its other side, a weakly conical bore 13 extends to the injection needle 8.
  • the receiving sleeve 10 essentially has the shape of the plug-in cone of the Luer type known from commercially available cannulas.
  • the insertion sleeve 11 has a conical outer wall 14 corresponding to the bore 13 and a continuous axial channel 15.
  • the channel 15 is widened at its end facing the receiving sleeve to a cylindrical chamber 16 which serves to receive the functional element 12.
  • a funnel-shaped extension 17 is formed, by means of which the insertion of the hollow needle 4 is facilitated when the cannula is replaced.
  • the central part 18 of the channel 15 is weakly conical or cylindrical and so narrow that it tightly encloses the hollow needle of the syringe.
  • a constriction 19 is provided in front of the chamber to limit the depth of insertion of the hollow needle.
  • the closed sides of the chamber 16 and the bore 13 are recessed conically. This results in 12 flat, free cavities on both sides of the functional element used.
  • the functional element 12 is a filter, for example. This is used to filter a sample when it is drawn up in order to separate undesired components of the sample. In order to avoid entrainment of the filtered components during the subsequent injection of such a filtered sample into an analysis device, the cannula used for drawing up is expediently removed or replaced by a new cannula with or without a filter.
  • the flat cavities on both sides of the functional element 12 achieve a uniform distribution of the sample over the entire active cross section.
  • the functional element 12 can also be a package with an active phase, on which components of the sample are adsorbed. These components can be redissolved in a subsequent step using a suitable solvent. As with filtering, these steps are possible both when opening and when ejecting.
  • a pipette provided with this cannula can therefore be used very advantageously for sample preparation.
  • Samples which are taken up and ejected treated by one or two different cannulas are dispensed into a vessel, for example a cuvette of a microtiter plate, and further processed there by mixing etc. before they are entered into an analysis device.
  • sample pipette according to the invention is evident in the fact that the liquid volume after being drawn up in the hollow needle is on the other side of the cannula. This makes it possible to exchange the cannula for another cannula before ejecting it.
  • the attachment part can also take forms other than those shown in the present exemplary embodiment.
  • cannulas can also be provided, the attachment part of which has a larger internal volume.
  • a liquid volume can only be drawn into the cannula. This can be expedient, for example, if there is already a sample inside the hollow needle and a mixture is to take place when it is ejected.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention concerns sample pipettes used in particular for injecting samples into high pressure liquid chromatography apparatuses, which are provided with a hollow needle (4) for drawing a sample and expelling same into a container or into a sample receiving device of the apparatus. An interchangeable cannula (7) is mounted on the hollow needle of such a pipette, said cannula being capable of containing additional functional elements (12) such as filters, chemically active phases and the like.

Description

Probenpipettedropper
Die Erfindung betrifft eine Probenpipette mit einer Hohlnadel zum Aufziehen eines Flüssigkeitsvolumens, beispielsweise einer Probe, eines Reagenz etc., und zum Ausstossen derselben in ein Gefäss oder in einen Probenaufnahmeeingang eines Analysengeräts, insbesondere für die Probeneinspritzung in ein HPLC-Gerät.The invention relates to a sample pipette with a hollow needle for drawing up a volume of liquid, for example a sample, a reagent, etc., and for ejecting it into a vessel or into a sample receiving inlet of an analysis device, in particular for sample injection into an HPLC device.
Zwischen einer Probenentnahme und der Eingabe der Probe in ein Chromatographiegerät ist häufig eine Probenvorbereitung erforderlich. Für die dazu nötigen Bearbeitungsschritte, wie Filtern, Abtrennen oder Zufügen einzelner Bestandteile etc. uss die Probe aus der Entnahmepipette in ein Bearbei- tungsgefäss ausgestossen und nach der Bearbeitung in eine Probenaufgabepipette aufgezogen werden.Sample preparation is often required between taking a sample and entering it into a chromatography device. For the processing steps required for this, such as filtering, separating or adding individual components, etc., the sample is ejected from the removal pipette into a processing vessel and, after processing, drawn up into a sample delivery pipette.
Eine Vielzahl von Bearbeitungsschritten ist aufwendig und erhöht das Risiko von Fehlern, Kontamination etc. Deshalb besteht auch im Bereich der Probenvorbereitung grundsätzlich ein Bedürfnis nach Vereinfachung. Dieses Bedürfnis liegt der Erfindung zugrunde .A large number of processing steps is complex and increases the risk of errors, contamination, etc. Therefore, there is also a fundamental need for simplification in the area of sample preparation. The invention is based on this need.
Erfindungsge äss wird eine Vereinfachung erreicht durch eine auf die Hohlnadel aufgesetzte auswechselbare mit funktioneilen Elementen, beispielsweise Filtern, Beschichtungen mit aktiven Phasen etc. versehene Kanüle aus einer Injektionsnadel und einem Ansatzteil.According to the invention, a simplification is achieved by an interchangeable cannula fitted with functional elements, for example filters, coatings with active phases, etc., made of an injection needle and an attachment part, which is placed on the hollow needle.
In der Medizin und in verwandten Gebieten wird unter dem Begriff Kanüle in der Regel eine einseitig zum Einstechen geeignete und am anderen Ende mit einem Steckkonus zur Verbindung mit einer Spritze etc. versehene Injektionsnadel verstanden. In der vorliegenden Beschreibung wird der Begriff in ähnlicher Weise verwendet, wobei anstelle des Steckkonus ein spezieller Ansatzteil vorhanden ist. Zur Unterscheidung von der Hohlnadel der Spritze wird für diejenige der Kanüle der Begriff Injektionsnadel verwendet.In medicine and in related areas, the term cannula is usually one-sided for piercing suitable injection needle provided at the other end with a plug-in cone for connection to a syringe etc. The term is used in a similar manner in the present description, with a special attachment part being present instead of the plug-in cone. To distinguish it from the hollow needle of the syringe, the term injection needle is used for that of the cannula.
Im folgenden werden anhand der beiliegenden Zeichnungen Ausführungsbeispiele der Erfindung beschrieben. Es zeigenExemplary embodiments of the invention are described below with reference to the accompanying drawings. Show it
Fig. 1 eine Probenaufgabepipette mit einer aufgesetzten auswechselbaren Kanüle Fig. 2 eine Schnittdarstellung der zusammengesetzten KanüleFig. 1 shows a sample pipette with a replaceable cannula attached. Fig. 2 is a sectional view of the assembled cannula
Fig. 3 eine Schnittdarstellung der auseinandergezogenen Einzelteile der KanüleFig. 3 is a sectional view of the exploded individual parts of the cannula
Fig. 1 zeigt eine Probenaufgabepipette in Form einer Spritze für die manuelle Probenaufgabe in ein HPLC-Gerät. Die Pipette besteht im wesentlichen aus einem Zylinder 1, an dessen einem Ende 2 eine mit seinem Innenraum 3 verbundene Hohlnadel 4 angeordnet ist, und einem im Innenraum axial bewegbaren Kolben 5, der vom anderen Ende 6 des Zylinders her betätigt wird. Pipetten, die nicht manuell betätigt werden, sondern Teil eines maschinell betriebenen Systems sind, unterscheiden sich davon im Grundsatz nur unwesent- lieh. Deshalb ist die nachfolgend beschriebene Neuerung ohne weiteres auf ein maschinell betriebenes System übertragbar.1 shows a sample application pipette in the form of a syringe for manual sample application in an HPLC device. The pipette consists essentially of a cylinder 1, at one end 2 of which a hollow needle 4 connected to its interior 3 is arranged, and a piston 5 which is axially movable in the interior and is actuated from the other end 6 of the cylinder. In principle, pipettes that are not operated manually but are part of a machine-operated system differ only marginally. Therefore, the innovation described below can easily be transferred to a machine-operated system.
Auf dem freien Ende der Hohlnadel 4 ist eine Kanüle 7 ange- ordnet, die lediglich aufgesteckt ist und daher leicht aus- weclselbar ist. Die Kanüle 7 ist für den einmaligen Gebrauch konzipiert, d.h. es wird in der Regel für jede neue Proloe eine neue Kanüle verwendet. Für gewisse Arbeits- schxitte kann es sinnvoll sein, die Kanüle sogar zwischen dem Aufziehen und dem Ausstossen einer Probe zu wechseln, wie nachfolgend beschrieben wird.A cannula 7 is arranged on the free end of the hollow needle 4, which is merely plugged on and therefore easily is changeable. The cannula 7 is designed for single use, ie a new cannula is generally used for each new proloe. For certain work steps it may make sense to change the cannula even between drawing up and ejecting a sample, as described below.
Die in den Schnittdarstellungen in Fig. 2 und 3 gezeigte Kanüle 7 besteht aus Injektionsnadel 8 und einem Ansatzteil 9. Der Ansatzteil 9 besteht aus einer Aufnahmehülse 10, einer koaxial in dieser angeordneten Einsteckhülse 11 und einem dazwischen angeordneten funktioneilen Element 12.The cannula 7 shown in the sectional views in FIGS. 2 and 3 consists of an injection needle 8 and an attachment part 9. The attachment part 9 consists of a receiving sleeve 10, an insertion sleeve 11 arranged coaxially therein and a functional element 12 arranged in between.
Die Aufnahmehülse 10 ist einseitig fest mit der Injektions- nadel 8 verbunden. Von ihrer anderen Seite erstreckt sich eine schwach konische Bohrung 13 bis zur Injektionsnadel 8. Die Aufnahmehülse 10 besitzt im wesentlichen die Form des von handelsüblichen Kanülen bekannten Steckkonus vom Luer- Typ - Der Ein achheit halber können daher solche handelsüb- liehen Kanülen verwendet werden.The receiving sleeve 10 is firmly connected on one side to the injection needle 8. From its other side, a weakly conical bore 13 extends to the injection needle 8. The receiving sleeve 10 essentially has the shape of the plug-in cone of the Luer type known from commercially available cannulas.
Die Einsteckhülse 11 besitzt eine der Bohrung 13 entsprechenden konische Aussenwand 14 und einen durchgängigen axialen Kanal 15. Der Kanal 15 ist an seinem der Aufnahme- hülse zugewandten Ende zu eine zylindrischen Kammer 16 erweitert, die zur Aufnahme des funktioneilen Elements 12 dient . Am anderen Ende des Kanals 15 ist eine trichterförmige Erweiterung 17 ausgebildet, durch welche beim Auswechseln der Kanüle die Einführung der Hohlnadel 4 erleichtert wird. Zwischen der Kammer 16 und der Erweiterung 17 ist der Mittelteil 18 des Kanals 15 schwach konisch oder zylindrisch und so eng, dass er die Hohlnadel der Spritze dicht umschliesst. Zur Begrenzung der Einführungstiefe der Hohlnadel ist vor der Kammer eine Engstelle 19 vorgesehen. Die geschlossenen Seiten der Kammer 16 und der Bohrung 13 sind konisch vertieft. Dadurch ergeben sich auf beiden Seiten des eingesetzten funktionellen Elements 12 flache frei bleibende Hohlräume.The insertion sleeve 11 has a conical outer wall 14 corresponding to the bore 13 and a continuous axial channel 15. The channel 15 is widened at its end facing the receiving sleeve to a cylindrical chamber 16 which serves to receive the functional element 12. At the other end of the channel 15, a funnel-shaped extension 17 is formed, by means of which the insertion of the hollow needle 4 is facilitated when the cannula is replaced. Between the chamber 16 and the extension 17, the central part 18 of the channel 15 is weakly conical or cylindrical and so narrow that it tightly encloses the hollow needle of the syringe. A constriction 19 is provided in front of the chamber to limit the depth of insertion of the hollow needle. The closed sides of the chamber 16 and the bore 13 are recessed conically. This results in 12 flat, free cavities on both sides of the functional element used.
Das funktioneile Element 12 ist beispielsweise ein Filter. Dieser dient dazu, eine Probe beim Aufziehen zu filtern, um unerwünschte Bestandteile der Probe abzutrennen. Um bei der nachfolgenden Einspritzung einer derart gefilterten Probe in ein Analysengerät die Mitnahme der abgefilterten Bestandteile zu vermeiden, wird die zum Aufziehen benutzte Kanüle zweckmässigerweise entfernt bzw. durch eine neue Kanüle mit oder ohne Filter ersetzt. Durch die flachen Hohlräume auf beiden Seiten des funktionellen Elements 12 wird eine gleichmässige Verteilung der Probe auf den gesamten aktiven Querschnitt erreicht.The functional element 12 is a filter, for example. This is used to filter a sample when it is drawn up in order to separate undesired components of the sample. In order to avoid entrainment of the filtered components during the subsequent injection of such a filtered sample into an analysis device, the cannula used for drawing up is expediently removed or replaced by a new cannula with or without a filter. The flat cavities on both sides of the functional element 12 achieve a uniform distribution of the sample over the entire active cross section.
Es ist auch möglich, eine Probe ohne Filter aufzuziehen und danach eine Kanüle mit Filter aufzusetzen, um die Probe bei der Probeneinspritzung zu filtern.It is also possible to draw up a sample without a filter and then to attach a cannula with a filter to filter the sample during the sample injection.
Das funktioneile Element 12 kann auch eine Packung mit einer aktiven Phase sein, an welcher Bestandteile der Probe adsorbiert werden. Diese Bestandteile können in einem an- schliessenden Schritt durch ein geeignetes Lösungsmittel -wieder gelöst werden. Wie beim Filtern sind diese Arbeitsschritte sowohl beim Aufziehen als auch beim Ausstossen möglich.The functional element 12 can also be a package with an active phase, on which components of the sample are adsorbed. These components can be redissolved in a subsequent step using a suitable solvent. As with filtering, these steps are possible both when opening and when ejecting.
Durch Kombination der verschiedenen Schritte und Elemente lässt sich eine Vielzahl von Behandlungen der Proben verwirklichen. Eine mit dieser Kanüle versehene Pipette kann daher sehr vorteilhaft für die Probenvorbereitung einge- setzt werden. Proben, welche beim Aufziehen und Ausstossen durch eine oder zwei verschiedene Kanülen behandelt sind, werden in ein Gefäss, beispielsweise eine Küvette einer Mi- krotiterplatte, abgegeben und dort durch Mischen etc. weiterbehandelt, bevor sie in ein Analysengerät eingegeben werden.By combining the different steps and elements, a variety of treatments of the samples can be realized. A pipette provided with this cannula can therefore be used very advantageously for sample preparation. Samples which are taken up and ejected treated by one or two different cannulas are dispensed into a vessel, for example a cuvette of a microtiter plate, and further processed there by mixing etc. before they are entered into an analysis device.
Ein wesentlicher Vorteil der erfindungsgemässen Probenpipette besteht ersichtlich darin, dass sich das Flüssigkeitsvolumen nach dem Aufziehen in der Hohlnadel auf der anderen Seite der Kanüle befindet . Dadurch ist es möglich, die Kanüle vor dem Ausstossen gegen eine andere Kanüle auszutauschen.A significant advantage of the sample pipette according to the invention is evident in the fact that the liquid volume after being drawn up in the hollow needle is on the other side of the cannula. This makes it possible to exchange the cannula for another cannula before ejecting it.
Selbstverständlich kann das Ansatzteil auch andere als die im vorliegenden Ausführungsbeispiel gezeigten Formen annehmen. Für spezielle Schritte im Rahmen der Probenbehandlung können auch Kanülen vorgesehen werden, deren Ansatzteil ein grösseres Innenvolumen aufweist. Bei Verwendung einer solchen Kanüle kann ein Flüssigkeitsvolumen lediglich in die Kanüle aufgezogen werden. Dies kann beispielsweise zweckmä- ssig sein, wenn sich bereits eine Probe im Inneren der Hohlnadel befindet und beim Ausstossen eine Mischung erfolgen soll. Of course, the attachment part can also take forms other than those shown in the present exemplary embodiment. For special steps in the context of sample treatment, cannulas can also be provided, the attachment part of which has a larger internal volume. When using such a cannula, a liquid volume can only be drawn into the cannula. This can be expedient, for example, if there is already a sample inside the hollow needle and a mixture is to take place when it is ejected.

Claims

Pa.tentansprüche Pa.tentansprüche
1. Probenpipette mit einer Hohlnadel zum Aufziehen eines FlüssigkeitsVolumens, beispielsweise einer Probe, eines Reagenz etc., und zum Ausstossen derselben in ein Gefäss oder in einen Probenaufnahmeeingang eines Analysengeräts, insbesondere für die Probeneinspritzung in ein HPLC-Gerät, gekennzeichnet durch eine auf die Hohlnadel (4) aufgesetzte auswechselbare Kanüle (7) .1.Sample pipette with a hollow needle for drawing up a volume of liquid, for example a sample, a reagent, etc., and for ejecting it into a vessel or into a sample inlet of an analytical device, in particular for sample injection into an HPLC device, characterized by one on the hollow needle (4) attached replaceable cannula (7).
2. Probenpipette nach Anspruch 1, dadurch gekennzeichnet, dass die Kanüle (7) aus einer Injektionsnadel (8) und einem Ansatzteil (9) besteht.2. Sample pipette according to claim 1, characterized in that the cannula (7) consists of an injection needle (8) and an attachment part (9).
3. Probenpipette nach Anspruch 2, dadurch gekennzeichnet, dass im Ansatzteil (9) der Kanüle ein funktionelles Element (12) angeordnet ist.3. Sample pipette according to claim 2, characterized in that a functional element (12) is arranged in the attachment part (9) of the cannula.
4. Probenpipette nach Anspruch 2, dadurch gekennzeichnet, dass im Ansatzteil (9) Kanüle ein die Hohlnadel (4) abdichtender Kanal (15) vorhanden ist.4. Sample pipette according to claim 2, characterized in that in the attachment part (9) cannula a the hollow needle (4) sealing channel (15) is present.
5. Probenpipette nach Anspruch 3, dadurch gekennzeichnet, dass das funktioneile Element (12) ein Filter ist.5. sample pipette according to claim 3, characterized in that the functional element (12) is a filter.
6. Probenpipette nach Anspruch 3, dadurch gekennzeichnet, dass das funktioneile Element (12) eine chemisch aktive Phase ist. 6. sample pipette according to claim 3, characterized in that the functional element (12) is a chemically active phase.
7. Kanüle zur Verwendung als auswechselbarer Aufsatz auf eine Probenpipette gekennzeichnet durch ein funktionales Element .7. Cannula for use as an exchangeable attachment on a sample pipette characterized by a functional element.
8. Kanüle nach Anspruch 7, dadurch gekennzeichnet, dass das funktionale Element ein Filter ist.8. Cannula according to claim 7, characterized in that the functional element is a filter.
9. Kanüle nach Anspruch 7, dadurch gekennzeichnet, dass das funktionale Element eine chemisch aktive Phase enthält. 9. Cannula according to claim 7, characterized in that the functional element contains a chemically active phase.
EP04789822A 2003-10-31 2004-10-29 Sample pipette Withdrawn EP1682275A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH18582003 2003-10-31
PCT/CH2004/000654 WO2005042166A1 (en) 2003-10-31 2004-10-29 Sample pipette

Publications (1)

Publication Number Publication Date
EP1682275A1 true EP1682275A1 (en) 2006-07-26

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US (1) US7434483B2 (en)
EP (1) EP1682275A1 (en)
WO (1) WO2005042166A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1697736B1 (en) * 2003-12-09 2009-01-07 BGB Analytik AG Device for sample preparation
US7798021B2 (en) 2006-01-12 2010-09-21 Gamble Kimberly R Method and apparatus for sample processing and injection
US8291778B2 (en) * 2006-09-08 2012-10-23 Ronald Rockwell Rich Extractive sampling system for fluids
CN101792105B (en) * 2009-02-04 2012-09-26 刘伟耀 Quantitative liquid getting device
WO2013021101A1 (en) 2011-08-08 2013-02-14 Thermo Fisher Scientific Oy Method and apparatus for automated analysis
FI20115785A0 (en) * 2011-08-08 2011-08-08 Thermo Fisher Scientific Oy Method and apparatus for automatic analysis
DE102012015063B4 (en) * 2012-07-31 2014-10-30 Sartorius Stedim Biotech Gmbh Apparatus and method for treating a filtration medium
CN112206844A (en) * 2020-08-17 2021-01-12 武汉迈特维尔生物科技有限公司 Device and method for filtering small-volume biological sample solution

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4001929A (en) 1975-07-30 1977-01-11 Soji Ishikawa Method of constructing a medical needle holder assembly
US4316462A (en) 1980-05-21 1982-02-23 Siloam, Inc. Filtering device for an injection device
JPS5861251U (en) * 1981-10-22 1983-04-25 石川　惣二 Needle
EP0201611A1 (en) * 1985-05-10 1986-11-20 B. Braun-SSC AG Two cannulae syringe
DE60228698D1 (en) 2001-04-20 2008-10-16 Gl Sciences Inc METHOD AND DEVICE FOR TRACE ELEMENT EXTRACTION IN THE SOLID PHASE
EP1446669A1 (en) 2001-10-26 2004-08-18 Advion BioSciences, Inc. Method and device for chemical analysis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2005042166A1 *

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WO2005042166A1 (en) 2005-05-12
US20070056350A1 (en) 2007-03-15
US7434483B2 (en) 2008-10-14

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