EP1682275A1 - Sample pipette - Google Patents

Sample pipette

Info

Publication number
EP1682275A1
EP1682275A1 EP20040789822 EP04789822A EP1682275A1 EP 1682275 A1 EP1682275 A1 EP 1682275A1 EP 20040789822 EP20040789822 EP 20040789822 EP 04789822 A EP04789822 A EP 04789822A EP 1682275 A1 EP1682275 A1 EP 1682275A1
Authority
EP
European Patent Office
Prior art keywords
sample
cannula
characterized
pipette
functional element
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP20040789822
Other languages
German (de)
French (fr)
Inventor
Hansjörg Cueni
Heiner Scherrer
Original Assignee
Hansjörg Cueni
Heiner Scherrer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CH18582003 priority Critical
Application filed by Hansjörg Cueni, Heiner Scherrer filed Critical Hansjörg Cueni
Priority to PCT/CH2004/000654 priority patent/WO2005042166A1/en
Publication of EP1682275A1 publication Critical patent/EP1682275A1/en
Application status is Withdrawn legal-status Critical

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/0275Interchangeable or disposable dispensing tips
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • G01N30/04Preparation or injection of sample to be analysed
    • G01N30/16Injection
    • G01N30/18Injection using a septum or microsyringe
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02Adapting objects or devices to another
    • B01L2200/026Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0832Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838Capillaries
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/021Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/022Capillary pipettes, i.e. having very small bore

Abstract

The invention concerns sample pipettes used in particular for injecting samples into high pressure liquid chromatography apparatuses, which are provided with a hollow needle (4) for drawing a sample and expelling same into a container or into a sample receiving device of the apparatus. An interchangeable cannula (7) is mounted on the hollow needle of such a pipette, said cannula being capable of containing additional functional elements (12) such as filters, chemically active phases and the like.

Description

dropper

The invention relates to a sample pipette with a hollow needle for drawing a volume of liquid, for example, a sample, a reagent, etc., and for ejecting the same into a container or into a sample receiving input of an analyzer, in particular for sample injection into a HPLC apparatus.

Between a sampling and the input of the sample into a chromatograph, a sample preparation is often required. For this the necessary processing steps, such as filtering, separating or addition of individual components etc. uss ejected the sample from the sampling pipette into a machining vessel and raised after processing in a sample application pipette.

A plurality of processing steps is time-consuming and increases the risk of errors, contamination, etc. Therefore, also in the field of sample preparation in principle a need for simplification. This need is based on the invention.

Erfindungsge AESS is a simplification achieved by a patch on the hollow needle exchangeable with functional elements, such as filters, coatings with active phases, etc. provided needle from a hypodermic needle and a shoulder portion.

In medicine and related fields in a side suitable for piercing and at the other end with a plug-in cone for connection to a syringe, etc., provided the injection needle is meant by the term cannula in the rule. In the present specification, the term is used in a similar manner using a special attachment part is provided in place of the plug-in cone. To distinguish it from the hollow needle cannula of the syringe of the term is used injection needle for that.

In the following, with reference to the accompanying drawings, embodiments of the invention are described. Show it

Fig. 1 is a sample application pipette with an attached interchangeable cannula Fig. 2 is a sectional view of the assembled cannula

Fig. 3 is a sectional view of the exploded parts of the cannula

Fig. 1 shows a sample application pipette in the form of a syringe for manual sample injection into a HPLC apparatus. The pipette consists essentially of a cylinder 1 having at one end 2, connected to its inner space 3 hollow needle 4 is disposed, and an axially movable in the interior piston 5 which is actuated from the other end 6 of the cylinder forth. Pipettes, which are not manually operated but are part of a mechanically operated system, which differ only insignificantly lent in principle. Therefore, the innovation described below readily on a machine-powered system is transferable.

On the free end of the hollow needle 4 is a hollow needle 7 is appropriate assigns, which is merely plugged and therefore off easily is weclselbar. The cannula 7 is designed for single use, ie, it is generally used for each new Proloe a new cannula. For certain work schxitte it may make sense to change the needle even between the mounting and the ejection of a sample, as described below.

The cannula 7 shown in the sectional views in Figs. 2 and 3 consists of injection needle 8 and a shoulder part 9. The shoulder part 9 consists of a receiving sleeve 10, a coaxially arranged therein insertion sleeve 11 and arranged therebetween, a functional element 12.

The receiving sleeve 10 on one side firmly connected to the injection needle. 8 From the other side, a slightly conical bore 13 extends to the injection needle 8. The holding sleeve 10 has substantially the form of the known commercially available cannulas plug cone of the Luer-type - The A achheit half can be used therefore, such standard commercial loan cannulas.

The insertion sleeve 11 has a bore 13 corresponding conical outer wall 14 and a continuous axial channel 15. The channel 15 is, at its receiving sleeve end facing to a cylindrical chamber 16 extends, which serves to receive the functional element 12th At the other end of the channel 15 a funnel-shaped extension 17 is formed, through which the introduction of the hollow needle 4 is facilitated when replacing the cannula. Between the chamber 16 and the extension 17 of the central portion 18 of the channel 15 is slightly conical or cylindrical and so close that it tightly surrounds the hollow needle of the syringe. To limit the depth of insertion of the hollow needle a constriction 19 is provided in front of the chamber. The closed sides of the chamber 16 and the bore 13 are tapered in depth. This results on both sides of the functional element 12 used flat remaining free cavities.

The functional element 12 is for example a filter. This serves to filter a sample when pulling to separate unwanted components of the sample. In order to avoid the entrainment of the filtered out constituents at the following injection of such a filtered sample in an analytical device, the cannula used for raising conveniently removed or replaced by a new cannula, with or without a filter. By the flat cavities on both sides of the functional element 12 is a uniform distribution of the sample is achieved in the entire active cross-section.

It is also possible to raise a sample without the filter and then put on a cannula with filter to filter the sample during sample injection.

The functional element 12 may also be a package with an active phase, at which components of the sample adsorbed. These ingredients may be dissolved in an on-closing step by a suitable solvent -again. As with filters, these operations are possible both during mounting and during ejection.

By combining the various steps and elements are a variety of treatments of the samples can be realized. A cannula provided with this pipette can therefore be set einge- very advantageous for the sample preparation. Samples which are treated in the drawing and expelling by one or two different cannulas are krotiterplatte in a vessel such as a cuvette of a micro discharged and there further treated by mixing, etc., before they are input into an analysis device.

An essential advantage of the inventive sample pipette is apparent that the volume of liquid is located after mounting in the hollow needle on the other side of the cannula. This makes it possible to replace the needle before the ejection against another cannula.

Of course, the approach portion may take other than the shapes shown in this embodiment. Special steps in the sample treatment also cannulas can be provided, the neck portion having a greater internal volume. When using such a cannula, a volume of liquid can be simply mounted into the cannula. This may be expedient, for example, if there is already a sample inside the hollow needle and should be performed when ejecting a mixture.

Claims

Pa.tentansprüche
1. Sample pipette with a hollow needle for drawing a volume of liquid, for example, a sample, a reagent, etc., and for ejecting the same into a container or into a sample receiving input of an analyzer, in particular for sample injection into a HPLC apparatus, characterized by the hollow needle (4) patch interchangeable cannula (7).
2. Sample A pipette according to claim 1, characterized in that the cannula (7) consists of an injection needle (8) and a neck part (9).
3. Sample A pipette according to claim 2, characterized in that in the neck part (9) of the cannula a functional element (12) is arranged.
4. Sample pipette according to claim 2, characterized in that in the neck part (9), a cannula, the hollow needle (4) a sealing passage (15) is present.
5. Sample A pipette according to claim 3, characterized in that the functional element (12) is a filter.
6. Sample pipette according to claim 3, characterized in that the functional element (12) is a chemically active phase.
7. cannula for use as a replaceable attachment to a sample pipette characterized by a functional element.
8. A cannula according to claim 7, characterized in that the functional element is a filter.
9. Cannula according to claim 7, characterized in that the functional element contains a chemically active phase.
EP20040789822 2003-10-31 2004-10-29 Sample pipette Withdrawn EP1682275A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CH18582003 2003-10-31
PCT/CH2004/000654 WO2005042166A1 (en) 2003-10-31 2004-10-29 Sample pipette

Publications (1)

Publication Number Publication Date
EP1682275A1 true EP1682275A1 (en) 2006-07-26

Family

ID=34529363

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20040789822 Withdrawn EP1682275A1 (en) 2003-10-31 2004-10-29 Sample pipette

Country Status (3)

Country Link
US (1) US7434483B2 (en)
EP (1) EP1682275A1 (en)
WO (1) WO2005042166A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1697736B1 (en) * 2003-12-09 2009-01-07 BGB Analytik AG Device for sample preparation
US7798021B2 (en) 2006-01-12 2010-09-21 Gamble Kimberly R Method and apparatus for sample processing and injection
US8291778B2 (en) * 2006-09-08 2012-10-23 Ronald Rockwell Rich Extractive sampling system for fluids
CN101792105B (en) * 2009-02-04 2012-09-26 刘伟耀 Quantitative liquid getting device
DE102012015063B4 (en) * 2012-07-31 2014-10-30 Sartorius Stedim Biotech Gmbh Apparatus and method for treating a filtration medium

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4001929A (en) * 1975-07-30 1977-01-11 Soji Ishikawa Method of constructing a medical needle holder assembly
US4316462A (en) * 1980-05-21 1982-02-23 Siloam, Inc. Filtering device for an injection device
JPS6125805Y2 (en) * 1981-10-22 1986-08-04
EP0201611A1 (en) * 1985-05-10 1986-11-20 B. Braun-SSC AG Two cannulae syringe
DE60228698D1 (en) 2001-04-20 2008-10-16 Gl Sciences Inc Method and device for trace element extraction in the solid phase
EP1446669A1 (en) 2001-10-26 2004-08-18 Advion BioSciences, Inc. Method and device for chemical analysis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2005042166A1 *

Also Published As

Publication number Publication date
WO2005042166A1 (en) 2005-05-12
US20070056350A1 (en) 2007-03-15
US7434483B2 (en) 2008-10-14

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Legal Events

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Effective date: 20060531

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Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR

DAX Request for extension of the european patent (to any country) deleted
17Q First examination report

Effective date: 20120525

18D Deemed to be withdrawn

Effective date: 20141025