WO1982001564A1 - Reactif pour l'analyse de l'activite d'une gamma-glutamyle transpeptidase - Google Patents

Reactif pour l'analyse de l'activite d'une gamma-glutamyle transpeptidase Download PDF

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Publication number
WO1982001564A1
WO1982001564A1 PCT/JP1981/000303 JP8100303W WO8201564A1 WO 1982001564 A1 WO1982001564 A1 WO 1982001564A1 JP 8100303 W JP8100303 W JP 8100303W WO 8201564 A1 WO8201564 A1 WO 8201564A1
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WO
WIPO (PCT)
Prior art keywords
substrate
activity
reagent
cyclodextrin
solution
Prior art date
Application number
PCT/JP1981/000303
Other languages
English (en)
Japanese (ja)
Inventor
Kagaku Kk Kanto
Original Assignee
Kawaji Haruhiko
Ono Toshihiro
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kawaji Haruhiko, Ono Toshihiro filed Critical Kawaji Haruhiko
Publication of WO1982001564A1 publication Critical patent/WO1982001564A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Definitions

  • the present invention relates to a test sample for measuring the activity of glutamyl transpeptidase. More specifically, the present invention is characterized in that it contains r-glutamyl-4-nitronitronide and cyclodextrin. The present invention relates to a reagent for measuring the activity of r-glutamyl transpeptidase.
  • the present inventors have conducted various studies on a method for measuring the enzymatic activity of O-OTP using Glut-4-NA as a substrate, and found that the reaction system had a cyclodextrin. It has been found that the addition of C can significantly increase the solubility of Olu-4-NA and also inhibit the non-excitable degradation of C-lu-4-NA in water or buffer solutions. did. The present invention has been made based on such knowledge. Is
  • r-glutamyl-4- 4-trao-lide (substrate) is used as a reagent for measuring the enzymatic activity of r-ciTP, to which cyclodextrin is added.
  • This is a feature, and its purpose is to use r-daltamyl- as a substrate.
  • Another object of the present invention is to provide a test method capable of remarkably and accurately measuring the activity of daltamyl transdibutidase as compared with the prior art.
  • Glu-4-NA used as a substrate has a solubility in water or buffer of up to 4 mM at most, as described above.
  • a high solubility of 10 m (4 times higher) can be obtained by adding, for example, six dextrin 0.3 (wv).
  • cyclodextrin has the effect of stabilizing C-lu-4-1NA in addition to the above-mentioned increase in solubility of Olu-14-NA. That is, when the aqueous solution or buffer solution (4 mLi) of Glu-4 -'-: ⁇ is left at room temperature, the decomposition amount has already reached about 27 AM in 24 hours and about 5 in 48 hours. It shows a value of about 80 M at 72 hours at 72 hours and about 108 # ⁇ at 90 hours, but this decomposition is due to this decomposition.] 4-nitronitrolin produced is r -After 24 hours, to prevent the catalysis of, already? "-It was found that the enzyme activity of -CT?
  • Cost and water solubility are recommended as the preferred modes of use for ⁇ -cyclodextrin.
  • An advantage to be noted with respect to the reagent of the present invention is that it can be provided as a lyophilized product. That is, conventionally, the r-GTP enzyme activity assay containing G1U-4-NA as a main component has been described above.3) Since Gin-4-NA is hardly soluble, various solubilization means are used. Finally, it was only possible to obtain a concentration of about 4 m, and it was almost impossible to produce a freeze-dried product as a prototype, but according to the present invention, By using in combination with cyclodextrin], it has become possible to provide freeze-dried products. That is, (r-GTP can be measured by a simple operation using a freeze-dried mixed solution of Jlu-4-NA and cyclodextrin. This will provide significant advantages in making the task of measuring 7 "-GTP significantly easier.
  • Buffer solution Glycyl Glycine 6.2 and diethanolamine 10.5 ⁇ are dissolved in about 900% of distilled water, and the pH is adjusted using saline. After adding & 2 (301C), distilled water was added to give 100 ⁇ .
  • Substrate solution Dissolve L-7 * -Gnoretamyl-4-nitrofuranilide 4. ⁇ and cyclodextrin 2.7 ⁇ with the above buffer solution.
  • Measurement solution 300 ml of the buffer solution of the above H) and the substrates v, The solution is mixed with to obtain a solution.
  • the amount of increase in absorbance per minute for 1 minute is defined as X.
  • Unit (activity) 1 00 ⁇ ⁇ 2 ⁇ 2 ⁇
  • the resulting lyophilized product is dissolved in water or buffer solution (3) to give a 16 mM Glu-4-NA solution.
  • the above freeze-dried product is much more stable than the solid state of Glu-4-HA alone]], and this product can be stored in a stable state.
  • the reagent for measuring the activity of 7 * -glutamyl transpeptidase of the present invention is r-glutamyl transpeptidase (r
  • the reagent for measuring r-GTP activity of the present invention is usefully used for medical diagnosis and also for enzymatic chemistry research, its industrial applicability is extremely large. You.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Reactif pour l'analyse de l'activite d'une gamma-glutamyle transpeptidase, contenant de la cyclodextrine avec un gamma-glutamyle-4-nitroanilide utilise en tant que substrat. La cyclodextrine sert a augmenter la solubilite du substrat et reduit la decomposition non enzymatique de celui-ci.
PCT/JP1981/000303 1980-10-28 1981-10-28 Reactif pour l'analyse de l'activite d'une gamma-glutamyle transpeptidase WO1982001564A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP55150240A JPS5774099A (en) 1980-10-28 1980-10-28 Reagent for measuring activity of gamma-cultanyl transpeptidase
JP80/150240801028 1980-10-28

Publications (1)

Publication Number Publication Date
WO1982001564A1 true WO1982001564A1 (fr) 1982-05-13

Family

ID=15492619

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP1981/000303 WO1982001564A1 (fr) 1980-10-28 1981-10-28 Reactif pour l'analyse de l'activite d'une gamma-glutamyle transpeptidase

Country Status (3)

Country Link
JP (1) JPS5774099A (fr)
DE (1) DE3152458C2 (fr)
WO (1) WO1982001564A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983000876A1 (fr) * 1981-08-28 1983-03-17 American Hospital Supply Corp Procede d'augmentation de la sensibilite d'analyses
US4511651A (en) * 1982-07-30 1985-04-16 American Monitor Corporation Reagent composition and assay for the determination of γ-glutamyltransferase activity

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2590983B2 (ja) * 1987-12-11 1997-03-19 東ソー株式会社 難水溶性抗原の免疫測定方法
JPH04365706A (ja) * 1991-06-13 1992-12-17 Daifuku Co Ltd シャッター付き回転棚装置の制御方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5443791A (en) * 1977-09-13 1979-04-06 Seishin Seiyaku Kk Method and reagent for measuring hydrogen peroxide

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS51125046A (en) * 1974-11-22 1976-11-01 Ono Pharmaceut Co Ltd Process for preparing prostaglandin analogues
JPS6016599A (ja) * 1983-07-08 1985-01-28 Yatoron:Kk γ−グルタミルトランスペプチダ−ゼの活性測定用試薬

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5443791A (en) * 1977-09-13 1979-04-06 Seishin Seiyaku Kk Method and reagent for measuring hydrogen peroxide

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Gendai Kagaku, No. 96, (1979-3) P.29-31 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983000876A1 (fr) * 1981-08-28 1983-03-17 American Hospital Supply Corp Procede d'augmentation de la sensibilite d'analyses
US4451563A (en) * 1981-08-28 1984-05-29 Kaufman Richard A Method for increasing the sensitivity of assays
US4511651A (en) * 1982-07-30 1985-04-16 American Monitor Corporation Reagent composition and assay for the determination of γ-glutamyltransferase activity

Also Published As

Publication number Publication date
DE3152458C2 (fr) 1991-02-14
JPS5774099A (en) 1982-05-10
DE3152458T1 (fr) 1982-12-02
JPS6440B2 (fr) 1989-01-05

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