US20190046642A1 - Il-6 antagonist formulations and uses thereof - Google Patents

Il-6 antagonist formulations and uses thereof Download PDF

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US20190046642A1
US20190046642A1 US16/078,671 US201716078671A US2019046642A1 US 20190046642 A1 US20190046642 A1 US 20190046642A1 US 201716078671 A US201716078671 A US 201716078671A US 2019046642 A1 US2019046642 A1 US 2019046642A1
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formulation
antibody
seq
fragment
sequence
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Grigorios Zarbis-Papastoitsis
Patricia Lowden
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Hoffmann La Roche Inc
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Sesen Bio Inc
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39591—Stabilisation, fragmentation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39533—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals
    • A61K39/3955—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals against proteinaceous materials, e.g. enzymes, hormones, lymphokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/02—Inorganic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/16—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing nitrogen, e.g. nitro-, nitroso-, azo-compounds, nitriles, cyanates
    • A61K47/18—Amines; Amides; Ureas; Quaternary ammonium compounds; Amino acids; Oligopeptides having up to five amino acids
    • A61K47/183—Amino acids, e.g. glycine, EDTA or aspartame
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/22—Heterocyclic compounds, e.g. ascorbic acid, tocopherol or pyrrolidones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0048—Eye, e.g. artificial tears
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A61P27/14—Decongestants or antiallergics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C07K16/248—IL-6
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance

Definitions

  • the present invention relates to therapeutic compositions formulations, e.g., for interleukin-6 (IL-6) antagonists.
  • IL-6 interleukin-6
  • IL-6 is a pleiotropic cytokine with reported roles in inflammation, hematopoiesis, angiogenesis, cell differentiation, and neuronal survival.
  • formulations e.g., pharmaceutical compositions, e.g., stable aqueous formulations
  • IL-6 antagonists e.g., IL-6 antibody molecules, e.g., IL-6 antibodies or fragments thereof, as described in WO2014/074905
  • IL-6 antagonists e.g., IL-6 antibody molecules, e.g., IL-6 antibodies or fragments thereof, as described in WO2014/074905
  • IL-6 antagonists e.g., IL-6 antibody molecules, e.g., IL-6 antibodies or fragments thereof, as described in WO2014/074905
  • IL-6 receptors e.g., IL-6 receptors
  • a pharmaceutical formulation that includes 1 mg/ml to 100 mg/ml of an IL-6 antagonist, e.g., an anti-IL-6 antibody or fragment thereof.
  • the pharmaceutical formulation comprises 5 mg/ml to 50 mg/ml of an IL-6 antagonist, e.g., an IL-6 antibody or fragment thereof; and one or more, or all, of, a buffer (e.g., a buffering agent), a surfactant, and/or a tonicity agent.
  • a buffer e.g., a buffering agent
  • the pharmaceutical formulation comprises 5 mg/ml to 50 mg/ml of an IL-6 antibody or fragment thereof, a buffering agent, a surfactant, and two tonicity agents (e.g., a sugar and a salt).
  • the present disclosure features a formulation, e.g., a pharmaceutical formulation, comprising 1-100 mg/mL of an IL-6 antagonist.
  • the formulation comprises 5-50 mg/mL of an IL-6 antagonist.
  • the formulation comprises about 5 mg/mL of an IL-6 antagonist.
  • the formulation comprises about 50 mg/mL of an IL-6 antagonist thereof.
  • the IL-6 antagonist is an IL-6 antibody molecule, e.g., an IL-6 antibody or fragment thereof.
  • the present disclosure features a formulation, e.g., a pharmaceutical formulation, comprising 1-100 mg/mL of an IL-6 antibody or fragment thereof.
  • the formulation comprises 5-50 mg/mL of an IL-6 antibody or fragment thereof.
  • the formulation comprises 1-10 mg/mL of an IL-6 antibody or fragment thereof.
  • the formulation comprises 2-3 mg/mL of an IL-6 antibody or fragment thereof.
  • the formulation comprises 3-4 mg/mL of an IL-6 antibody or fragment thereof.
  • the formulation comprises 4-5 mg/mL of an IL-6 antibody or fragment thereof.
  • the formulation comprises 5-6 mg/mL of an IL-6 antibody or fragment thereof.
  • the formulation comprises 6-7 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 7-8 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 8-9 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 9-10 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 5 mg/mL, +/ ⁇ 10% of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 5 mg/mL, +/ ⁇ 20% of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 5 mg/mL, +/ ⁇ 30% of an IL-6 antibody or fragment thereof.
  • the formulation comprises 5 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 10-100 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 20-80 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 40-60 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 20-30 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 30-40 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 40-50 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 50-60 mg/mL of an IL-6 antibody or fragment thereof.
  • the formulation comprises 60-70 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 70-80 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 80-90 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 90-100 mg/mL of an IL-6 antibody or fragment thereof. In one embodiment, the formulation comprises 50 mg/mL of an IL-6 antibody or fragment thereof.
  • Exemplary IL-6 antibody molecules e.g., IL-6 antibodies and fragments thereof, are provided herein.
  • the formulation comprises a concentration of an IL-6 antibody molecule, e.g., IL-6 antibody or fragment thereof, which is between at least 10% less than and at least 10% greater than a concentration of IL-6 antibody molecule disclosed herein.
  • the formulation comprises 50 mg/mL+/ ⁇ 10% of an IL-6 molecule, e.g., an IL-6 antibody or fragment thereof.
  • the formulation comprises 50 mg/mL, +/ ⁇ 20% of an IL-6 antibody or fragment thereof.
  • the formulation comprises 50 mg/mL, +/ ⁇ 30% of an IL-6 antibody or fragment thereof.
  • the formulation further comprises 1-50 mM histidine buffer, e.g., 5-40 mM histidine buffer, 10-30 mM histidine buffer, or 15-25 mM histidine buffer. In one embodiment, the formulation further comprises 1-10 mM histidine buffer, 10-20 mM histidine buffer, 20-30 mM histidine buffer, 30-40 mM histidine buffer, 40-50 mM histidine buffer, 5-10 mM histidine buffer, 10-15 mM histidine buffer, 15-20 mM histidine buffer, 20-25 mM histidine buffer, 25-30 mM histidine buffer, 30-35 mM histidine buffer, 35-40 mM histidine buffer, 40-45 mM histidine buffer, or 45-50 mM histidine buffer.
  • 1-50 mM histidine buffer e.g., 5-40 mM histidine buffer, 10-30 mM histidine buffer, or 15-25 mM histidine buffer.
  • the formulation further comprises 1
  • the formulation further comprises 1 mM, 5 mM, 10 mM, 15 mM, 20 mM, 25 mM, 30 mM, 35 mM, 40 mM, 45 mM, or 50 mM histidine buffer.
  • the formulation comprises concentration of a buffer, e.g., a histidine buffer, which is between at least 10% less than and at least 10% greater than a concentration of a buffer disclosed herein.
  • the formulation comprises 20 mM+/ ⁇ 10% of histidine buffer.
  • the formulation further comprises 20 mM+/ ⁇ 20% histidine buffer.
  • the formulation further comprises 20 mM+/ ⁇ 30% histidine buffer.
  • the formulation further comprises 0.01% to 1% w/v polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188, e.g., 0.01% to 0.5%, 0.01% to 0.1%, 0.01% to 0.05%, 0.02% to 0.04% w/v polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188.
  • the formulation further comprises 0.01 to 0.02%, 0.02% to 0.03%, 0.03 to 0.04%, 0.04% to 0.05%, 0.05% to 0.06%, 0.07% to 0.08%, 0.08% to 0.09%, or 0.09% to 0.1% w/v polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188.
  • the formulation further comprises 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.1%, 0.15%, 0.2%, 0.5%, or 1% w/v polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188.
  • the formulation comprises concentration of a surfactant, e.g., polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188, which is between at least 10% less than and at least 10% greater than a concentration of a surfactant disclosed herein.
  • a surfactant e.g., polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188
  • the formulation comprises 0.03%+/ ⁇ 0.003% of polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188.
  • the formulation further comprises 0.03%+/ ⁇ 0.01% of polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188.
  • polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), and poloxamer 188 are interchangeable.
  • the formulation further comprises 1-150 mM sodium chloride, e.g., 1-50 mM, 1-25 mM, 5-100 mM, 10-75 mM, 10-50 mM, 10-30 mM, or 15-25 mM sodium chloride.
  • 1-150 mM sodium chloride e.g., 1-50 mM, 1-25 mM, 5-100 mM, 10-75 mM, 10-50 mM, 10-30 mM, or 15-25 mM sodium chloride.
  • the formulation further comprises 1-10 mM, 1-20 mM, 5-15 mM, 5-25 mM, 10-20 mM, 10-30 mM, 15-25 mM, 15-35 mM, 20-30 mM, 30-40 mM, 40-50 mM, 50-60 mM, 70-80 mM, 80-90 mM, 90-100 mM, 100-110 mM, 110-120 mM, 120-130 mM, 130-140 mM, or 140-150 mM sodium chloride.
  • the formulation further comprises about 1 mM, 5 mM, 10 mM, 15 mM, 20 mM, 25 mM, 30 mM, 50 mM, 100 mM, or 150 mM sodium chloride.
  • the formulation comprises concentration of sodium chloride, which is between at least 10% less than and at least 10% greater than a concentration of sodium chloride disclosed herein.
  • the formulation comprises 20 mM+/ ⁇ 10% of sodium chloride.
  • the formulation further comprises 20 mM+/ ⁇ 20% sodium chloride.
  • the formulation further comprises 20 mM+/ ⁇ 30% sodium chloride.
  • the formulation further comprises 1 to 10% sorbitol, e.g., 1 to 8%, 1 to 6%, 2 to 5%, or 3 to 5% sorbitol. In one embodiment, the formulation further comprises 1 to 10% sorbitol, 2% to 12%, 3% to 13%, 4% to 14%, 5% to 15%, 1% to 2%, 2%, to 3%, 3% to 4%, 4% to 5%, 5% to 6%, 6%, to 7%, 7% to 8%, 8% to 9%, or 9% to 10% sorbitol.
  • 1 to 10% sorbitol e.g., 1 to 8%, 1 to 6%, 2 to 5%, or 3 to 5% sorbitol.
  • the formulation further comprises 1 to 10% sorbitol, 2% to 12%, 3% to 13%, 4% to 14%, 5% to 15%, 1% to 2%, 2%, to 3%, 3% to 4%, 4% to 5%, 5% to
  • the formulation further comprises about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10% sorbitol.
  • the formulation comprises concentration of sorbitol, which is between at least 10% less than and at least 10% greater than a concentration of sorbitol disclosed herein.
  • the formulation comprises 4%+/ ⁇ 0.4% sorbitol.
  • the formulation further comprises 4%+/ ⁇ 0.8% sorbitol.
  • the formulation further comprises 4%+/ ⁇ 1.2% sorbitol.
  • the pH of the formulation is between about 5.5 and about 7.5, e.g., between about 5.5 and about 7.0, between about 6.0 and about 7.0, between about 6.2 and about 6.8. In one embodiment, the pH of the formulation is about 5.5, about 5.6, about 5.7, about 5.8, about 5.9, about 6.0, about 6.1, about 6.2, about 6.3, about 6.4, about 6.5, about 6.6, about 6.7, about 6.8, about 6.9, or about 7.0. In one embodiment, the pH of the formulation is about 6.5.
  • the present disclosure features a formulation, e.g., a pharmaceutical formulation, comprising 1-100 mg/ml or 5-50 mg/ml of an IL-6 antibody or fragment thereof as described herein, 10-50 mM histidine buffer; 0.01%-0.1% polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188; 1-150 mM sodium chloride; and 1-10% sorbitol; at a pH between 5.5 and 7.5, e.g., at a pH of 6.5.
  • a pharmaceutical formulation comprising 1-100 mg/ml or 5-50 mg/ml of an IL-6 antibody or fragment thereof as described herein, 10-50 mM histidine buffer; 0.01%-0.1% polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188; 1-150 mM sodium chloride; and 1-10% sorbitol; at a pH between 5.5 and 7.5, e.g.
  • the present disclosure features a formulation, e.g., a pharmaceutical formulation, comprising 5-50 mg/ml of an IL-6 antibody or fragment thereof as described herein; 10-30 mM histidine buffer; 0.01%-0.05% polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188; 10-30 mM sodium chloride; and 1-6% sorbitol; at pH between 6 and 7, e.g., at pH of 6.5.
  • a formulation e.g., a pharmaceutical formulation, comprising 5-50 mg/ml of an IL-6 antibody or fragment thereof as described herein; 10-30 mM histidine buffer; 0.01%-0.05% polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188; 10-30 mM sodium chloride; and 1-6% sorbitol; at pH between 6 and 7, e.g., at pH of 6.5.
  • the present disclosure features a formulation, e.g., a pharmaceutical formulation, comprising 5-50 mg/ml of an IL-6 antibody or fragment thereof as described herein; 15-25 mM histidine buffer; 0.02%-0.04% polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188; 15-25 mM sodium chloride; and 3-5% sorbitol; at pH between 6.2 and 6.8, e.g., at pH of 6.5.
  • a formulation e.g., a pharmaceutical formulation, comprising 5-50 mg/ml of an IL-6 antibody or fragment thereof as described herein; 15-25 mM histidine buffer; 0.02%-0.04% polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188; 15-25 mM sodium chloride; and 3-5% sorbitol; at pH between 6.2 and 6.8, e.g., at pH of 6.5.
  • the present disclosure features a formulation, e.g., a pharmaceutical formulation, comprising about 5-50 mg/mL of an anti-IL-6 antibody or fragment thereof as described herein; 20 mM histidine buffer, e.g., histidine HCl; 0.03% polysorbate-20 (Tween-20); 20 mM sodium chloride; and 4% sorbitol; at pH of 5.5.
  • a formulation e.g., a pharmaceutical formulation, comprising about 5-50 mg/mL of an anti-IL-6 antibody or fragment thereof as described herein; 20 mM histidine buffer, e.g., histidine HCl; 0.03% polysorbate-20 (Tween-20); 20 mM sodium chloride; and 4% sorbitol; at pH of 5.5.
  • the formulation comprises about 5 mg/mL of an anti-IL-6 antibody or fragment thereof; 20 mM histidine buffer, e.g., histidine HCl; 0.03% polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188; 20 mM sodium chloride; and 4% sorbitol, at a pH of 5.5.
  • the formulation comprises about 50 mg/mL of an anti-IL-6 antibody or fragment thereof; 20 mM histidine buffer, e.g., histidine HCl; 0.03% polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188; 20 mM sodium chloride; and 4% sorbitol, at pH of 5.5.
  • the present disclosure features a formulation, e.g., a pharmaceutical formulation, comprising about 5-50 mg/mL of an anti-IL-6 antibody or fragment thereof as described herein; 20 mM histidine buffer, e.g., histidine HCl; 0.03% polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188; 20 mM sodium chloride; and 4% sorbitol; at pH of 5.5.
  • a formulation e.g., a pharmaceutical formulation, comprising about 5-50 mg/mL of an anti-IL-6 antibody or fragment thereof as described herein; 20 mM histidine buffer, e.g., histidine HCl; 0.03% polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188; 20 mM sodium chloride; and 4% sorbitol; at pH of 5.5.
  • the formulation comprises about 5 mg/mL of an anti-IL-6 antibody or fragment thereof, 20 mM histidine buffer, e.g., histidine HCl; 0.03% polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188; 20 mM sodium chloride; and 4% sorbitol, at a pH of 5.5.
  • the formulation comprises about 50 mg/mL of an anti-IL-6 antibody or fragment thereof; 20 mM histidine buffer, e.g., histidine HCl; 0.03% polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), or poloxamer 188; 20 mM sodium chloride; and 4% sorbitol, at pH of 5.5.
  • the formulation may further comprise one or more, or all, of a chelating agent, a preserving agent, a viscosity agent, a penetration enhancer or bioadhesive, a stabilizer, and/or an antioxidant.
  • the formulation further comprises a second therapeutic agent.
  • the second therapeutic agent is an anti-VEGF agent, an anti-PGDF agent, or a steroid, e.g., a corticosteroid.
  • an the anti-VEGF agent or the anti-PDGF agent can be a small molecule, a peptide, an antibody, or a nucleic acid that inhibits or decreases the activity of the VEGF pathway.
  • the anti-VEGF agent is an antibody that inhibits or decreases the activity of a component of the VEGF pathway, e.g., VEGF or a the VEGF receptor.
  • the anti-PDGF agent is an antibody that inhibits or decreases the activity of a component of the PDGF pathway, e.g., PDGF or the PDGF receptor.
  • the formulation is stable at room temperature or less, e.g., at a temperature of about 20° C. or less. In one embodiment, the formulation is stable at 20° C. or less, 10° C. or less, 8° C. or less, 4° C. or less, 2° C. or less, ⁇ 20° C. or less, ⁇ 65° C. or less, ⁇ 80° C. or less, ⁇ 100° C. or less. In one embodiment, the formulation is stable between 2 and 20° C., e.g., 2-8° C.
  • the formulation is stable for at least 1 week, at least 2 weeks, at least 1 month, at least 2 months, at least 4 months, at least 6 months, at least 12 months, at least 16 months, at least 20 months, at least 24 months, or at least 36 months.
  • the formulation is stable at a temperature of ⁇ 65° C. or less for at least 1 or 2 years. In one embodiment, the formulation is stable at a temperature between 2 to 8° C. for at least 6 months.
  • the IL-6 antibody molecule comprises a sequence, or portions thereof, provided in Table 1 or FIG. 1A or 1B .
  • the IL-6 antibody molecule e.g., antibody or fragment thereof, comprises a VH CDR1 comprising the sequence of SEQ ID NO:19, a VH CDR2 comprising the sequence of SEQ ID NO:20, and a VH CDR3 comprising the sequence of SEQ ID NO:21.
  • the IL-6 antibody or fragment thereof further comprises a VL CDR1 comprising the sequence of SEQ ID NO:22, a VL CDR2 comprising the sequence of SEQ ID NO:23, and a VL CDR3 comprising the sequence of SEQ ID NO:24.
  • the IL-6 antibody or fragment thereof comprises a VH CDR1 comprising the sequence of SEQ ID NO:19, a VH CDR2 comprising the sequence of SEQ ID NO:20, and a VH CDR3 comprising the sequence of SEQ ID NO:21; and a VL CDR1 comprising the sequence of SEQ ID NO:22, a VL CDR2 comprising the sequence of SEQ ID NO:23, and a VL CDR3 comprising the sequence of SEQ ID NO:24.
  • the IL-6 antibody or fragment thereof comprises (e.g., consists of) a constant region sequence that is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to SEQ ID NO: 28 or SEQ ID NO: 29. In one embodiment, the IL-6 antibody or fragment thereof comprises (e.g., consists of) a constant region sequence comprising SEQ ID NO: 28 or SEQ ID NO: 29.
  • the IL-6 antibody or fragment thereof comprises (e.g., consists of) a heavy chain variable region sequence that is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to SEQ ID NO:17. In one embodiment, the IL-6 antibody or fragment thereof comprises (e.g., consists of) a heavy chain variable region sequence comprising SEQ ID NO:17.
  • the IL-6 antibody or fragment thereof comprises (e.g., consists of) a heavy chain sequence that is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to SEQ ID NO:13. In one embodiment, the IL-6 antibody or fragment thereof comprises (e.g., consists of) a heavy chain sequence comprising SEQ ID NO:13.
  • the IL-6 antibody or fragment thereof comprises (e.g., consists of) a light chain variable region sequence that is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to SEQ ID NO:18. In one embodiment, the IL-6 antibody or fragment thereof comprises (e.g., consists of) a light chain variable region sequence comprising SEQ ID NO:18, or
  • the IL-6 antibody or fragment thereof comprises (e.g., consists of) a light chain sequence that is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to SEQ ID NO:14. In one embodiment, the IL-6 antibody or fragment thereof comprises (e.g., consists of) a light chain sequence comprising SEQ ID NO:14.
  • the IL-6 antibody or fragment thereof comprises (e.g., consists of) a heavy chain variable region comprising SEQ ID NO:17 and a light chain variable region comprising SEQ ID NO:18. In one embodiment, the IL-6 antibody or fragment thereof comprises (e.g., consists of) comprises a heavy chain sequence comprising SEQ ID NO:13 and a light chain sequence comprising SEQ ID NO:14.
  • the anti-IL-6 antibody is an IgG2 antibody.
  • the IL-6 antibody is a full-length antibody.
  • IgG2 antibodies can exist in different structural isoforms due to alternative disulfide bonding between the heavy and light chains of the antibody, e.g., isoform IgG2-A, isoform IgG2-AB, and isoform IgG2-B, also referred to herein as isoform A, isoform A/B, or isoform B.
  • the structures of the isoforms are also shown in FIG. 11 .
  • At least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, of the antibody present in the formulation is in isoform A or A/B, collectively. In one embodiment, at least 70%, 75%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, of the antibody present in the formulation is in isoform A.
  • less than 5%, 4%, 3%, 2%, 1%, 0.9%, 0.8%, 0.7%, 0.6%, 0.5%, 0.4%, 0.3%, 0.2%, or 0.1%, of the antibody present in the formulation is in isoform B.
  • the formulation is substantially free of the antibody present in isoform B, e.g., less than 5%, less than 4%, less than 3%, less than 2%, less than 1%, or less than 0.5% of the antibody present is in isoform B.
  • the percentage, amount, or quantity of antibody present in the formulation in isoform A, A/B, and/or B can be determined by HPLC, e.g., reverse phase HPLC (RP-HPLC), or peptide mapping under non-reducing conditions, followed by mass spectrometry (MS) analysis.
  • HPLC e.g., reverse phase HPLC (RP-HPLC), or peptide mapping under non-reducing conditions, followed by mass spectrometry (MS) analysis.
  • compositions and methods for treating a subject having an IL-6-associated disease or disorder includes administering to the subject a therapeutically effective amount of a composition comprising a formulation described herein.
  • the method includes identifying a subject having an IL-6 associated disease or disorder described herein; and administering to the subject a therapeutically effective amount of a composition comprising a formulation as described herein.
  • Also described herein is a method of inhibiting IL-6 activity in a subject.
  • the method includes administering to the subject a formulation as described herein.
  • the subject has an IL-6-associated disease or disorder described herein.
  • compositions as described herein in the manufacture of a medicament for treating or preventing an IL-6 associated disease or disorder in a subject, e.g., in the manufacture of a medicament for suitable for administration to a subject for treating or preventing an IL-6 associated disease or disorder in the subject.
  • the medicament is for administration to the eye, e.g., ocular administration.
  • IL-6 associated diseases or disorders can be associated with increased or elevated IL-6 expression or activity.
  • one or more symptoms of the IL-6 associated disease or disorder is associated with increased or elevated IL-6 expression or activity.
  • Increased or elevated IL-6 expression can be determined in a subject as compared to the level of IL-6 expression prior to onset of the disease or a symptom of the disease.
  • Increased or elevated IL-6 expression can be determined in a subject as compared to another subject that does not have an IL-6 associated disease or disorder.
  • IL-6 associated diseases include, but are not limited to, diabetic macular edema (DME), diabetic retinopathy, dry eye (e.g., dry eye disease or dry eye syndrome), allergic conjunctivitis, uveitis, age-related macular degeneration (AMD) (e.g., wet (exudative) or dry (atrophic) AMD), proliferative diabetic retinopathy (PDR), Rhegmatogenous retinal detachment (RRD), retinal vein occlusion (RVO), neuromyelitis optica (NMO), corneal transplant, corneal abrasion, myopic choroidal neovascularization, ocular cancers (e.g., cancers affecting the eye or area around the eye, e.g., the eye socket and/or eyelids), or physical injury to the eye.
  • DME diabetic macular edema
  • DME diabetic macular edema
  • AMD age-related macular degeneration
  • a second therapeutic agent is administered to the subject in combination with a formulation as described herein.
  • the second therapeutic agent is administered in a separate composition than the formulation described herein.
  • the formulation described herein includes the second therapeutic agent.
  • Suitable second therapeutic agents include any therapeutic agent commercially available or known for treating an IL-6 associated disease or disorder described herein.
  • the second therapeutic agent is an anti-VEGF agent, an anti-PDGF agent, or a steroid.
  • the subject treated as described herein is a human or other mammal such as a dog or cat.
  • the subject has previously been treated with an anti-VEGF agent or a steroid.
  • the subject is resistant or refractory to anti-VEGF agent or steroid treatment, e.g., the subject did not respond to treatment with an anti-VEGF agent or steroid.
  • subjects that do not respond to a given treatment are those in which one or more symptoms of a disease or disorder, e.g., an IL-6 related disease, is not ameliorated or reduced after administration of the given treatment.
  • the present disclosure features a device, e.g., a drug delivery device, comprising a formulation as described herein.
  • the present disclosure features a container or device comprising a formulation as described herein.
  • the container is a multidose container.
  • the container holds a volume of 0.1 ml, 0.2 ml, 0.3 ml, 0.4 ml, 0.5 ml, 0.6 ml, 0.7 ml, 0.8 ml, 0.9 ml, or 1.0 ml.
  • the present disclosure features a kit comprising a formulation as described herein, and optionally, instructions for use.
  • the kit comprises one or more containers or devices comprising a formulation as described herein, and optionally, instructions for use.
  • an element means one element or more than one element.
  • FIG. 1A depicts the locations of FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4, CH1, hinge, CH2, and CH3 in the heavy chain sequences of EBI-029 (SEQ ID NO: 1), EBI-030 (SEQ ID NO: 13), and EBI-031 (EBI-031 is also referred to herein as EBI-030-H311A) (SEQ ID NO: 25).
  • FIG. 1B depicts the locations of FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4, and CK in light chain sequence (EBI-029, EBI-030 and EBI-031 have the same light chain sequence) (SEQ ID NO: 3).
  • FIGS. 2A and 2B are graphs showing the relationship between main peak purity (%), as determined by SE-UPLC analysis, with increasing pH according to the different buffers tested ( FIG. 2A ) or with the different excipients tested ( FIG. 2B ).
  • FIGS. 3A, 3B, 3C, 3D, and 3E show the results from the SE-UPLC analysis performed at day 7, and as described in Example 3.
  • FIG. 4 is a graph showing the relationship between main peak purity (%), as determined by SE-UPLC analysis, with increasing pH according to the different buffers tested. Each buffer tested is represented on the x-axis by the well designation from the 96 well plate layout as shown in FIG. 4 .
  • FIG. 5 is a graph showing the relationship between HMW peaks, as determined by SE-UPLC analysis, with increasing pH according to the different buffers tested. Each buffer tested is represented on the x-axis by the well designation from the 96 well plate layout as shown in FIG. 4 .
  • FIG. 6 is a graph showing the main peak purity (%) with respect to the different salt concentrations and excipients for the samples containing histidine buffer.
  • the numbers 1-12 represent the salt concentration and excipients present in columns 1-12 listed in FIG. 4 .
  • FIG. 7 summarizes the results from the first agitation study described in Example 4, and as determined by SE-UPLC analysis.
  • FIGS. 8A, 8B, and 8C summarizes the results from the second agitation study described in Example 4, and as determined by SE-UPLC analysis.
  • FIGS. 9A, 9B, 9C, and 9D are graphs showing the total peak area ( FIG. 9A ), IgG main peak purity ( FIG. 9B ), high molecular weight (HMW) species ( FIG. 9C ), and low molecular weight (LMW) species ( FIG. 9D ) with respect to each tested excipient under the different agitation conditions used.
  • FIG. 10 shows a schematic diagram of the three different structural isoforms of IgG2 antibodies due to disulfide shuffling.
  • FIG. 11 shows RP-HPLC chromatograms of EBI-031 samples: untreated (top panel), 5 mM DTT (middle panel), 10 mM cysteine (bottom panel).
  • FIG. 12 shows RP-HPLC chromatograms of EBI-031 samples collected from different EBI-031 cell lines: a 200 L scale culture of a clonal cell line (top panel), a 10 L scale culture from a parental cell line (middle panel), and a stably transfected pool of cells (bottom panel).
  • FIG. 13 shows the RP-HPLC chromatogram of EBI-031 collected from a 200 L scale culture of a clonal cell line, and designates and quantifies which isoforms are represented by each peak in the chromatogram.
  • formulations useful for providing an IL-6 antagonist, e.g., an IL-6 antibody or fragment thereof, to a subject in need of treatment with such a formulation In embodiments, the subject has, or is at risk of having, an IL-6 associated disease or disorder, e.g., a disease associated with elevated or increased IL-6 expression and/or activity. Also disclosed herein are methods of preparing and administering such formulations.
  • formulations described herein have been formulated and optimized to be suitable for administration to a patient and to provide improved stability at varying temperatures and prolonged periods of time.
  • the formulations described herein comprising an IL-6 antibody comprising a constant region derived from an IgG2 antibody, e.g., an IgG2 constant region described herein
  • the formulations comprise an optimal distribution of the different IgG2 structural isoforms, e.g., primarily isoform A and isoform A/B, and very little or negligible amounts of isoform B.
  • the structural isoform B may be associated with aggregation, decreased function, and decreased stability of the antibody.
  • compositions and formulations comprising the antibody of the invention are less heterogeneous than other compositions or formulations comprising IgG2 antibodies known in the art.
  • an IL-6 antagonist also referred to herein as IL-6a.
  • an IL-6 antagonist (IL-6a) described herein can bind to IL-6, and inhibits or reduces at least one IL-6 activity.
  • IL-6 activity can include one or more of the following: binding to gp130; activation of the IL-6 signaling pathway; activation of a JAK kinase, e.g., phosphorylation of a target of a JAK kinase; activation of a STAT protein, e.g., phosphorylation of a STAT protein; and/or expression of a STAT-target gene.
  • an IL-6a described herein specifically binds to site II (site 2) of an IL-6 and is useful for treatment of IL-6 related diseases, e.g., IL-6 related eye diseases and certain other diseases as described herein.
  • the IL-6a features one or more of the following properties: has high affinity for either free IL-6 (e.g., soluble IL-6) or bound IL-6 (e.g., IL-6 bound to an IL-6 receptor) or both free and bound IL-6; is relatively stable in an organism; can inhibit binding to gp130 of an IL-6 bound to an IL-6R (termed herein an IL-6/IL-6R complex or IL-6/IL-6R); and/or can have a therapeutic effect.
  • free IL-6 e.g., soluble IL-6
  • bound IL-6 e.g., IL-6 bound to an IL-6 receptor
  • both free and bound IL-6 e.g., IL-6
  • is relatively stable in an organism can inhibit binding to gp130 of an IL-6 bound to an IL-6R (termed herein an IL-6/IL-6R complex or IL-6/IL-6R); and/or can have a therapeutic effect.
  • the IL-6a is an antibody or is a fragment derived from an antibody.
  • an IL-6a is a high affinity, humanized Fab that can specifically bind to site II of an IL-6 and potently blocks both cis- and trans-IL-6 signaling.
  • the IL-6a is a full length antibody, e.g., an IgG1 or IgG2 antibody.
  • the IL-6a selectively binds to site II of IL-6 and provides broad inhibition of IL-6 signaling because such molecules can inhibit the binding of gp130 to IL-6, regardless of whether the IL-6 is free or bound to membrane IL-6R or sIL-6R. Furthermore, targeting the ligand (IL-6) as opposed to the IL-6 receptor can avoid receptor mediated clearance and toxicity due to ADCC (antibody-dependent cell-mediated cytotoxicity).
  • ADCC antibody-dependent cell-mediated cytotoxicity
  • IL-6 plays both pathologic and protective roles in disease
  • use of an IL-6 antagonist (IL-6a) to treat a disease associated with increased IL-6 can improve certain aspects of a condition, but may also cause significant adverse effects, e.g., systemic effects.
  • This duality of IL-6 pathways i.e., the ability to have desirable and/or undesirable effects
  • the compositions and methods provided herein can be useful for treatments that inhibit at least one IL-6 activity, but do not have an undue effect on positive activities of IL-6, in part because the compositions can be formulated for local delivery, e.g., for local delivery to the eye.
  • the IL-6a is designed to be of a size suitable for delivery to a particular site.
  • the IL-6a is a full-length antibody.
  • the IL-6a is derived from an antibody and is in a format that may have longer residency in a particular compartment of the eye, e.g., the vitreous of the eye, and limited systemic leakage.
  • the IL-6a is a modified antibody (e.g., an antibody with a modified Fc domain) that has longer residency in the vitreous of the eye and/or more limited systemic leakage compared with a corresponding unmodified antibody.
  • the IL-6a is an IgG2 antibody.
  • the IL-6a is a relatively small IL-6a such as a fragment of an IL-6 antibody or other derivative of an antibody that is less than a full length antibody, e.g., a Fab that is derived from an IL-6 antibody.
  • an IL-6a is in a format that can pass from one part of a tissue to another with increased kinetics compared to a corresponding full-length IL-6 antibody.
  • the IL-6a is a Fab that has been engineered to be a larger molecule, which is more likely to have increased residence in the location to which it was delivered compared to the Fab alone, e.g., the IL-6a is dimerized through Fc domain.
  • the Fc domain has been engineered such that the Fc moiety has ablated or reduced FcRn binding that can reduce systemic accumulation compared to the same IL-6 binding entity that includes a wild-type Fc.
  • the engineered Fc domain can be, e.g., an IgG1 domain or an IgG2 domain.
  • the IL-6 antagonists described herein have a sufficiently high affinity for their target, IL-6, to be effective in ameliorating at least one undesirable effect of IL-6 and are sufficiently stable to be useful as therapeutics.
  • the PK of an IL-6a e.g., an IL-6a suitable for use in the eye has a sufficiently long half life in the site of delivery, e.g., the vitreous, to provide a therapeutic effect.
  • the PK can be a half-life of at least 8 days, 10 days, 14 days, 21 days, 28 days, or 30 days.
  • any method known in the art can be used to generate a molecule that can bind to an IL-6, for example, polypeptide libraries or molecular libraries can be screened for candidate compounds in an assay for the ability of a polypeptide or compound to bind to IL-6. Once such a candidate compound is identified, the binding site of the compound can be determined using methods known in the art. For example, a molecule can be tested for the ability to bind to wild type IL-6 and the binding compared to the ability of the compound to bind to an IL-6 mutated in site I, site II, or site III.
  • an IL-6a as described herein retains the ability to bind to an IL-6/IL-6R ⁇ complex and to IL-6, and prevents binding of IL-6/IL-6R ⁇ to gp130.
  • an IL-6a as described herein can compete with gp130 for binding to IL-6/IL-6R ⁇ complex, e.g., by binding to site II of IL-6. Such binding activities can be assayed using methods known in the art.
  • IL-6a candidates can be tested, for example, using an HEK-BlueTM IL-6 assay system (InvivoGen, San Diego).
  • HEK-BlueTM IL-6 cells are HEK293 cells that are stably transfected with human IL-6R and a STAT3-inducible SEAP reporter gene. In the presence of IL-6, STAT3 is activated and SEAP is secreted. SEAP is assessed using, for example, QUANTI-BlueTM (InvivoGen, San Diego). Addition of an IL-6 antagonist to the cells prevents secretion or decreases the level of SEAP as a result of inhibiting both free and soluble receptor bound IL-6.
  • K D refers to the binding affinity equilibrium constant of a particular antibody-antigen interaction or antibody fragment-antigen interaction.
  • an antibody or antigen binding fragment described herein binds to an antigen (e.g., IL-6) with a K D that is less than or equal to 250 pM, e.g., less than or equal to 225 pM, 220 pM, 210 pM, 205 pM, 150 pM, 100 pM, 50 pM, 20 pM, 10 pM, or 1 pM.
  • K D can be determined using methods known in the art, for example using surface plasmon resonance, for example, using the BiaCoreTM system.
  • K off refers to the dissociation rate constant of a particular antibody-antigen interaction or antibody fragment-antigen complex.
  • the dissociation rate constant can be determined using surface plasmon resonance, for example using the BiaCoreTM system.
  • a relatively slow K off can contribute to desirable features of a therapeutic, e.g., permitting less frequent administration of the inhibitor to a subject in need of such treatment.
  • an IL-6a described herein binds specifically to a target, e.g., an IL-6.
  • a target e.g., an IL-6.
  • “specific binding” as used herein indicates that a molecule preferentially binds to a selected molecule and displays much lower binding affinity for one or more other molecules.
  • the binding affinity for another molecule is 1, 2, 3 or more orders of magnitude lower than the binding affinity for the target.
  • IL-6 can be present as free IL-6 and as IL-6 bound to soluble IL-6R ⁇ .
  • site II of IL-6 as an optimal target for an IL-6 antagonist compared to an inhibitor that that binds to site I of an IL-6.
  • a site I inhibitor may inhibit binding of free IL-6 to IL-6R ⁇ .
  • an inhibitor that binds to an IL-6R ⁇ is less suitable because it may have limited ability to prevent IL-6 activity unless it is present in saturating concentrations.
  • an advantage of an IL-6a as described herein is that relatively less of the composition may need to be administered to achieve a therapeutic effect compared to an inhibitor targeting an IL-6 receptor.
  • Anti-receptor antibodies have been reported to be cleared rapidly by receptor mediated clearance significantly limiting their PK, therefore requiring larger doses, more frequent dosing, or both. Additionally, both anti-receptor and anti-site I IL-6 antibodies pose a problem in that they significantly increase the tissue concentration of IL-6 by disrupting the normal receptor mediated clearance pathway of the ligand, thereby exposing the subject to potentially undesirable levels of IL-6 in a tissue. Furthermore, use of an inhibitor targeting IL-6R ⁇ may necessitate the presence of the inhibitor near both sites at which inhibition is sought and a site at which it is not desirable, e.g., systemic treatment.
  • an IL-6a that binds site II, the site to which gp130 binds permits inhibition via free IL-6 as well as IL-6 that is bound to an IL-6R, but has not yet activated an IL-6 pathway via gp130.
  • the IL-6 antagonists described herein are designed to bind to both forms of IL-6 (soluble and receptor bound), specifically the IL-6 antagonists bind to site II of IL-6, which is accessible in both forms.
  • Compositions containing an IL-6a as described herein can inhibit both cis and trans signaling by IL-6.
  • compounds and methods provided herein are designed to provide an effective IL-6 blockade sufficient to treat at least one sign or symptom of an IL-6 associated disorder, for example, inhibiting angiogenesis and/or inflammation.
  • Compounds described herein are useful for treating eye diseases characterized by an undesirably high level of IL-6, e.g., in the vitreous (see Yuuki et al., J Diabetes Compl 15:257 (2001); Funatsu et al., Ophthalmology 110: 1690, (2003); Oh et al., Curr Eye Res 35:1116 (2010); Noma et al., Eye 22:42 (2008); Kawashima et al., Jpn J Ophthalmol 51:100 (2007); Kauffman et al., Invest Ophthalmol Vis Sci 35:900 (1994); Miao et al., Molec Vis 18:574 (2012)).
  • an IL-6a as described herein is a potent antagonist of IL-6 signaling.
  • an IL-6a described herein has a high affinity for IL-6, for example, an IC50 less than or equal to 100 pM in an HEK-Blue IL-6 assay using 10 pM IL-6.
  • High affinity of an IL-6a can be determined based on the K D of the IL-6a, for example, a K D of less than or equal to 1 nM, less than or equal to 500 pM, less than or equal to 400 pM, less than or equal to 300 pM, less than or equal to 240 pM, or less than or equal to 200 pM.
  • a biologic IL-6a e.g., a protein or polypeptide such as an antibody, fragment, or derivative thereof
  • a suitable productivity is greater than or equal to 1 g/L (e.g., greater than or equal to 2 g/L, greater than or equal to 5 g/L, or greater than or equal to 10 g/L).
  • the inhibitor have solubility compatible with the concentration at which it will be administered.
  • the solubility is greater than or equal to 20 mg/ml, greater than or equal to 10 mg/ml, greater than or equal to 5 mg/ml, or greater than or equal to 1 mg/ml.
  • the inhibitor must have high stability at the body temperature of the delivery and activity sites as well as storage stability.
  • the inhibitor has a T m of greater than or equal to 60° C. (e.g., greater than or equal to 60° C., greater than or equal to 62.5° C., greater than or equal to 65° C., greater than or equal to 70° C., greater than or equal to 73° C., or greater than or equal to 75° C.).
  • the inhibitor has a T onset of greater than or equal to 45° C., e.g., greater than or equal to 50° C., greater than or equal to 51° C., greater than or equal to 55° C., or greater than or equal to 60° C. Methods of determining the T m and T onset can be determined using methods known in the art.
  • Antagonists having the desired features can be selected from suitable types of molecules known in the art, for example antibodies, including fragments and derivatives of an IL-6 site II targeted antibody that generally retains or maintains sufficient features of the parent IL-6 antibody (e.g., desired binding properties).
  • Such antagonists include F ab fragments, scFvs, F ab fragments engineered to include an Fc moiety, and full-length antibodies engineered to have a framework different from the parent IL-6 site II targeted antibody.
  • the IL-6a disclosed herein comprises a human antibody antigen-binding site that can compete or cross-compete with an antibody or fragment thereof that can bind to site II of IL-6.
  • the antibody or fragment thereof can be composed of a VH domain and a VL domain disclosed herein, and the VH and VL domains comprise a set of CDRs of an IL-6/site II binding antibody disclosed herein.
  • Any suitable method may be used to determine the domain and/or epitope bound by an IL-6a, for example, by mutating various sites on an IL-6. Those sites in which mutations prevent or decrease binding of the IL-6a and the IL-6 ligand are involved either directly in binding to the IL-6a or indirectly affect the binding site, e.g., by affecting conformation of the IL-6.
  • Other methods can be used to determine the amino acids bound by an IL-6a.
  • a peptide-binding scan can be used, such as a PEPSCAN-based enzyme linked immuno assay (ELISA). In a peptide-binding scan of this type, short overlapping peptides derived from the antigen are systematically screened for binding to a binding member.
  • the peptides can be covalently coupled to a support surface to form an array of peptides.
  • Peptides can be in a linear or constrained conformation.
  • a constrained conformation can be produced using peptides having a terminal cysteine (cys) residue at each end of the peptide sequence.
  • the cys residues can be covalently coupled directly or indirectly to a support surface such that the peptide is held in a looped conformation.
  • a peptide used in the method may have a cys residue added to each end of a peptide sequence corresponding to a fragment of the antigen.
  • Double looped peptides can also be used, in which a cys residue is additionally located at or near the middle of the peptide sequence.
  • the cys residues can be covalently coupled directly or indirectly to a support surface such that the peptides form a double-looped conformation, with one loop on each side of the central cys residue.
  • Peptides can be synthetically generated, and cys residues can therefore be engineered at desired locations, despite not occurring naturally in the IL-6 site II sequence.
  • linear and constrained peptides can both be screened in a peptide-binding assay.
  • a peptide-binding scan may involve identifying (e.g., using an ELISA) a set of peptides to which the binding member binds, wherein the peptides have amino acid sequences corresponding to fragments of an IL-6a (e.g., peptides that include about 5, 10, or 15 contiguous residues of an IL-6a), and aligning the peptides in order to determine a footprint of residues bound by the binding member, where the footprint comprises residues common to overlapping peptides.
  • the peptide-binding scan method can be used to identify peptides to which the IL-6a binds with at least a selected signal:noise ratio.
  • an IL-6a useful as described herein is a human antibody molecule, a humanized antibody molecule, or binding fragment thereof.
  • the antibody is a monoclonal antibody.
  • the origin of such an antibody can be human, murine, rat, camelid, rabbit, ovine, porcine, or bovine and can be generated according to methods known to those in the art.
  • antibody molecule refers to a protein, or polypeptide sequence derived from an immunoglobulin molecule which specifically binds with an antigen.
  • the antibody molecule can be a full-length antibody or a fragment thereof, e.g., an antigen binding fragment thereof.
  • Antibodies can be polyclonal or monoclonal, multiple or single chain, or intact immunoglobulins, and may be derived from natural sources or from recombinant sources. Antibodies can be tetramers of immunoglobulin molecules.
  • Antibody fragments or antigen binding fragments refer to at least one portion of an intact antibody, or recombinant variants thereof, and refers to the antigen binding domain, e.g., an antigenic determining variable region of an intact antibody, that is sufficient to confer recognition and specific binding of the antibody fragment to a target, such as an antigen.
  • antibody fragments include, but are not limited to, Fab, Fab′, F(ab′) 2 , and Fv fragments, scFv antibody fragments, linear antibodies, single domain antibodies such as sdAb (either VL or VH), camelid VHH domains, and multi-specific antibodies formed from antibody fragments such as a bivalent fragment comprising two Fab fragments linked by a disulfide brudge at the hinge region, and an isolated CDR or other epitope binding fragments of an antibody.
  • An antigen binding fragment can also be incorporated into single domain antibodies, maxibodies, minibodies, nanobodies, intrabodies, diabodies, triabodies, tetrabodies, v-NAR and bis-scFv (see, e.g., Hollinger and Hudson, Nature Biotechnology 23:1126-1136, 2005).
  • Antigen binding fragments can also be grafted into scaffolds based on polypeptides such as a fibronectin type III (Fn3)(see U.S. Pat. No. 6,703,199, which describes fibronectin polypeptide minibodies).
  • an IL-6a comprises at least the CDRs of an antibody that can specifically bind to an IL-6 (e.g., a human IL-6), e.g., to site II of an IL-6.
  • the structure for carrying a CDR or a set of CDRs of the invention can be an antibody heavy or light chain sequence or substantial portion thereof in which the CDR or set of CDRs is located at a location corresponding to the CDR or set of CDRs of naturally occurring VH and VL antibody variable domains encoded by rearranged immunoglobulin genes.
  • the structures and locations of immunoglobulin variable domains can be determined by reference to Kabat, et al., 1983 (National Institutes of Health), and updates thereof findable under “Kabat” using any internet search engine.
  • An IL-6a is typically an antibody molecule that generally comprises an antibody VH domain and/or VL domain.
  • a VH domain comprises a set of heavy chain CDRs (VHCDRs), and a VL domain comprises a set of light chain CDRs (VLCDRs). Examples of such CDRS are provided herein in the Examples.
  • An antibody molecule can comprise an antibody VH domain comprising a VHCDR1, VHCDR2 and VHCDR3 and a framework. It can alternatively or also comprise an antibody VL domain comprising a VLCDR1, VLCDR2 and VLCDR3 and a framework.
  • IL-6 antagonists comprising a VHCDR1 and/or VHCDR2 and/or VHCDR3 such as those disclosed herein and/or a VLCDR1 and/or VLCDR2 and/or VLCDR3 such as those disclosed herein.
  • the IL-6a can comprise one or more, e.g., one, two, or three, CDRs of any of the antibodies, fragments or derivatives described herein.
  • the IL-6a can comprise a set of VHCDRs (e.g., VHCDR1, VHCDR2, and/or VHCDR3), and optionally it can also comprise a set of VLCDRs (e.g., VLCDR1, VLCDR2, and/or VLCDR3).
  • the CDRs can be derived from one or more antibodies, fragments, or derivatives described herein.
  • the VLCDRs can be derived from the same or a different antibody as the VHCDRs.
  • a VH domain is paired with a VL domain to provide an antibody antigen-binding site.
  • the HC domain of SEQ ID NO:1, SEQ ID NO:13 or SEQ NO:25 is paired with the LC domain of SEQ ID NO:3.
  • a VH or VL domain alone can be used as an IL-6a.
  • an isolated antibody or antigen binding fragment comprising a heavy chain variable region comprising
  • VH CDR1 comprising the sequence of GYX 1 LX 2 NYLIE (SEQ ID NO:30)
  • VH CDR2 comprising the sequence of VX 3 TPGX 4 GTIN (SEQ ID NO:31)
  • X 1 is not A
  • X 2 is not S
  • X 3 is not I
  • X 4 is not S.
  • X 1 is not A
  • X 2 is not S
  • X 3 is not I
  • X 4 is not S.
  • X 1 is V or a conservative substitution for V.
  • X 2 is P or a conservative substitution for P.
  • X 3 is T or a conservative substitution for T.
  • X 4 is G or a conservative substitution for G.
  • one, two, three or all of the following is true: X 1 is V or a conservative substitution for V, X 2 is P or a conservative substitution for P, X 3 is T or a conservative substitution for T, and X 4 is G or a conservative substitution for G.
  • X 1 is V or a conservative substitution for V
  • X 2 is P or a conservative substitution for P
  • X 3 is T or a conservative substitution for T
  • X 4 is G or a conservative substitution for G.
  • X 1 is selected from V, I, L and M. In embodiments, X 1 is selected from V, I and L. In embodiments, X 2 is selected from P, G, and A. In embodiments, X 2 is selected from P and G. In embodiments, X 3 is selected from T and S. In embodiments, X 4 is selected from G and P.
  • X 1 is V
  • X 2 is P
  • X 3 is T
  • X 4 is G
  • X 1 is V
  • X 2 is P
  • X 3 is T
  • X 4 is G.
  • X 1 is V
  • X 2 is P
  • X 3 is T
  • X 4 is G.
  • the VH CDR3 comprises the sequence of SEQ ID NO:21.
  • the antibody molecule e.g., antibody or antigen binding fragment
  • the antibody or antigen binding fragment has increased affinity for human IL-6 and/or increased potency compared with an antibody or antigen binding fragment (e.g., an otherwise identical antibody or antigen binding fragment) comprising a sequence wherein one or more (e.g., 1, 2, 3, or all) of the following is true: X 1 is A, X 2 is S, X 3 is I and X 4 is S.
  • the isolated antibody molecule e.g., antibody or antigen binding fragment thereof, comprises a VH CDR1 comprising the sequence of SEQ ID NO:7, a VH CDR2 comprising the sequence of SEQ ID NO:8, and optionally a VH CDR3 comprising the sequence of SEQ ID NO:9.
  • the isolated antibody or antibody fragment thereof differs by no more than 3, 2, or 1 amino acids in each of one, two, or all of the CDRs, e.g., SEQ ID NO: 7, SEQ ID NO: 8, or SEQ ID NO: 9.
  • the isolated antibody molecule e.g., antibody or antigen binding fragment thereof, comprises a VH CDR1 comprising the sequence of SEQ ID NO:19, a VH CDR2 comprising the sequence of SEQ ID NO:20, and optionally a VH CDR3 comprising the sequence of SEQ ID NO:21.
  • the isolated antibody or antibody fragment thereof differs by no more than 3, 2, or 1 amino acids in each of one, two, or all of the CDRs, e.g., SEQ ID NO: 19, SEQ ID NO: 20, or SEQ ID NO: 21.
  • the isolated antibody molecule e.g., antibody or antigen binding fragment thereof, comprises a VL CDR1 comprising the sequence of SEQ ID NO:9 or 22, a VL CDR2 comprising the sequence of SEQ ID NO:10 or 23, and a VL CDR3 comprising the sequence of SEQ ID NO:11 or 24.
  • the isolated antibody or antibody fragment thereof differs by no more than 3, 2, or 1 amino acids in each of one, two, or all of the CDRs, e.g., SEQ ID NO: 22, SEQ ID NO: 23, or SEQ ID NO: 24.
  • the heavy chain variable region comprises a sequence that is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% identical with SEQ ID NO:5.
  • the heavy chain variable region consists of a sequence is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% identical with SEQ ID NO:5 or differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:5.
  • the heavy chain variable region differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:5, wherein the amino acid changes are not in any of the CDRs.
  • the heavy chain variable region differs by 1-5 amino acids from SEQ ID NO:5.
  • the heavy chain variable region comprises a sequence that is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% identical to SEQ ID NO:17. In embodiments, the heavy chain variable region consists of a sequence is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% identical to SEQ ID NO:17. In embodiments, the heavy chain variable region differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:17.
  • the heavy chain variable region differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:17, wherein the amino acid changes are not in any of the CDRs.
  • the antibody or antigen binding fragment comprises a heavy chain variable region sequence comprising SEQ ID NO:17. In embodiments, the antibody or antigen binding fragment comprises a heavy chain variable region sequence consisting of SEQ ID NO:17.
  • the light chain variable region comprises a sequence that is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% identical with SEQ ID NO:18.
  • the light chain variable region consists of a sequence is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% identical with SEQ ID NO:18 or differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:18.
  • the light chain variable region differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:18, wherein the amino acid changes are not in any of the CDRs.
  • the light chain variable region differs by 1-5 amino acids from SEQ ID NO:18.
  • the antibody molecule e.g., antibody or antigen binding fragment
  • the antibody or antigen binding fragment comprises a sequence that is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% identical to SEQ ID NO:15.
  • the antibody or antigen binding fragment comprises a sequence that differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:15.
  • the antibody or antigen binding fragment differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:15, wherein the amino acid changes are not in any of the CDRs.
  • the antibody or antigen binding fragment comprises SEQ ID NO:15.
  • the antibody or antigen binding fragment is a Fab.
  • the antibody molecule e.g., antibody or antigen binding fragment
  • the antibody or antigen binding fragment comprises a sequence that is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% identical to SEQ ID NO:16.
  • the antibody or antigen binding fragment comprises a sequence that differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:16.
  • the antibody or antigen binding fragment differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:16, wherein the amino acid changes are not in any of the CDRs.
  • the antibody or antigen binding fragment comprises SEQ ID NO:16.
  • the antibody or antigen binding fragment is a Fab.
  • the antibody molecule e.g., antibody or antigen binding fragment
  • the antibody or antigen binding comprises or consists of the scFv sequence provided in SEQ ID NO:26 or SEQ ID NO:27.
  • the antibody or antigen binding fragment comprises a sequence that is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% identical to SEQ ID NO:26 or SEQ ID NO:27.
  • the antibody or antigen binding fragment differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:26 or 27.
  • the antibody or antigen binding fragment differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:26 or 27, wherein the amino acid changes are not in any of the CDRs.
  • the antibody or antigen binding fragment comprises SEQ ID NO:26 or SEQ ID NO:27.
  • the antibody or antigen binding fragment is an scFv.
  • the antibody molecule e.g., antibody or antigen binding fragment
  • the antibody or antigen binding fragment comprises a heavy chain sequence that differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:13.
  • the heavy chain sequence differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:13, wherein the amino acid changes are not in any of the CDRs.
  • the antibody or antigen binding fragment comprises a heavy chain sequence comprising SEQ ID NO:13.
  • the antibody or antigen binding fragment comprises a heavy chain sequence consisting of SEQ ID NO:13.
  • the antibody molecule e.g., antibody or antigen binding fragment
  • the antibody or antigen binding fragment comprises a light chain sequence that differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:14.
  • the light chain sequence differs by no more than 20, 15, 10, 5, 4, 3, 2, or 1 amino acids from SEQ ID NO:14, wherein the amino acid changes are not in any of the CDRs.
  • the antibody or antigen binding fragment comprises a light chain sequence comprising SEQ ID NO:14.
  • the antibody or antigen binding fragment comprises a light chain sequence consisting of SEQ ID NO:14.
  • the antibody molecule e.g., antibody or antigen binding fragment
  • the antibody or antigen binding fragment comprises one or more sequences of EBI-029, EBI-030, or EBI-031 as provided in Table 1.
  • the antibody or antigen binding fragment comprises one or more domains of EBI-030 or EBI-031 as shown in FIG. 1 (e.g., one or more of FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4, CH1, hinge, CH2, and CH3 of the heavy chain sequence and/or FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4, and CK of the light chain sequence).
  • the antibody or antigen binding fragment comprises a heavy chain and a light chain.
  • the heavy and light chains are linked by one or more disulfide bonds.
  • the antibody or antigen binding fragment is a Fab.
  • the antibody or antigen binding fragment is an scFv.
  • the antibody or antigen binding fragment is Fab, Fab′, F(ab′)2, scFv or Fv fragment.
  • the antibody molecule e.g., antibody or antigen binding fragment
  • antibody or antigen binding fragment has increased affinity for human IL-6 and/or increased potency compared with tocilizumab.
  • the IL-6a is an antibody molecule, fragment, or derivative thereof that comprises (i) a VH domain sequence that has at least 60, 70, 80, 85, 90, 95, 98 or 99% amino acid sequence identity with a VH domain described herein (e.g., SEQ ID NO:17), or (ii) a set of VHCDRs (e.g., VHCDR1, VHCDR2, and/or VHCDR3) from the VH domain sequence.
  • the antibody molecule, fragment, or derivative thereof comprises a VHCDR1, VHCDR2, and VHCDR3 of SEQ ID NO:17.
  • the antibody molecule, fragment, or derivative thereof comprises a VHCDR1, VHCDR2, and VHCDR3 that collectively differ from the VHCDR1, VHCDR2, and VHCDR3 of SEQ ID NO:17 by no more than 1, no more than 2, no more than 3, no more than 4, or no more than 5 amino acids.
  • the antibody molecule, fragment, or derivative thereof can optionally also comprise (i) a VL domain sequence that has at least 60, 70, 80, 85, 90, 95, 98 or 99% amino acid sequence identity with a VL domain described herein, e.g., a VL domain of SEQ ID NO: 18, or (ii) a set of VLCDRs (e.g., VLCDR1, VLCDR2, and/or VLCDR3) from the VL domain.
  • the antibody molecule, fragment or derivative thereof comprises VLCDR1, VLCDR2, and VLCDR3 of SEQ ID NO: 18.
  • the antibody molecule, fragment, or derivative comprises a VLCDR1, VLCDR2, and VLCDR3 that collectively differ from the VLCDR1, VLCDR2, and VLCDR3 of SEQ ID NO:18 by no more than 1, no more than 2, no more than 3, no more than 4, or no more than 5 amino acids.
  • Algorithms that can be used to calculate percent identity of two amino acid sequences include e.g., BLAST, FASTA, or the Smith-Waterman algorithm, e.g., employing default parameters.
  • An IL-6a as described herein can comprise antibody constant regions or parts thereof, e.g., human antibody constant regions or parts thereof.
  • a VL domain may be attached at its C-terminal end to antibody light chain constant domains including human CK or CL chains.
  • an IL-6a based on a VH domain can be attached at its C-terminal end to all or part (e.g., a CH1 domain) of an immunoglobulin heavy chain derived from any antibody isotype, e.g. IgG, IgA, IgE and IgM and any of the isotype sub-classes, particularly IgG1, IgG2, IgG3 and IgG4.
  • the antibody or antigen binding fragment is engineered to reduce or eliminate ADCC activity.
  • the antibody of the invention is an IgG2 antibody.
  • the antibody of the invention comprises an IgG2 framework, IgG2 constant region, or IgG2 Fc region as described herein.
  • IgG2 antibodies can exist as three major structural isoforms: IgG2-A, IgG2-B, and IgG2-AB (Wypych J. et al. Journal of Biological Chemistry. 2008, 283:16194-16205).
  • This structural heterogeneity is due to different configurations of the disulfide bonds that link the Fab arms to the heavy chain hinge region.
  • IgG2-A isoform there are no disulfide bonds linking the Fab arms to the hinge region.
  • IgG2-B isoform both Fab arms have disulfide bonds linking the heavy and light chain to the hinge region.
  • the IgG2-AB isoform is a hybrid between the IgG2-A and IgG2-B isoforms, with only one Fab arm having disulfide bonds linking the heavy and light chain of the one Fab arm to the hinge region.
  • the conversion of an IgG2 antibody between two or all of the different structural isoforms, also referred to as disulfide shuffling, occurs naturally in vivo and in vitro for both naturally-occurring and recombinant antibodies.
  • formulations of IgG2 antibodies in the art comprise a heterogeneous mixture of IgG2-A, IgG2-B, and IgG2-AB isoforms.
  • the different IgG2 isoforms can have unique and different functional properties, such as differences in stability, aggregation, viscosity, Fc receptor binding, or potency. Presence of multiple isoforms or increased levels of a particular isoform in a IgG2 antibody formulation can negatively affect stability, aggregation, or potency.
  • Some fragments of an IgG2 antibody that can still undergo disulfide shuffling and exist in any of the structural isoforms A, A/B, and/or B can be readily envisioned, e.g., fragments that retain the residues that participate in the shuffling disulfide bonds (e.g., as shown in FIG. 10 ), e.g., the fragment comprises at least an IgG2 hinge region.
  • the present invention provides formulations comprising an antibody or a fragment thereof with the advantage of primarily existing in the IgG2-A or IgG2-AB isoform.
  • the antibody or a fragment thereof does not exist in the IgG2-B isoform, or does not exist in the IgG2-B isoform for a substantial amount of time, thereby resulting in a very low level of IgG2-B isoform in a composition or formulation at a given time.
  • compositions and formulations comprising the antibody described herein are less heterogeneous than other IgG2 antibodies known in the art, and therefore, more preferred for use in a therapeutic application.
  • compositions and formulations comprising the antibody comprise primarily IgG2-A and/or IgG2-AB isoforms of the antibody.
  • a composition comprising an antibody described herein comprises at least 50, 60, 70, 80, 90, 95, 96, 97, 98, or 99% of the IgG2-A or IgG2-AB isoforms of the antibody.
  • a composition comprising an antibody described herein comprises at least 60, 70, 80, 90, 95, 96, 97, 98, or 99% of the IgG2-A and IgG2-AB isoforms collectively.
  • a composition comprising an antibody described herein does not comprise a substantial amount of the IgG2-B isoforms of the antibody.
  • the composition comprises less than 10%, 5%, 2%, 1%, 0.5%, or 0.1% of the IgG2-B isoforms of the antibody.
  • an antibody of the invention is further modified using methods known in the art create a sequence having a specific allotype, for example an allotype that predominates in a population having a particular geographic origin.
  • the human heavy chain constant region is modified for this purpose.
  • An IL-6a can be an antibody molecule, binding fragment thereof, or variant, having one or more CDRs, for example, a set of CDRs, within an antibody framework.
  • one or more CDRs or a set of CDRs of an antibody may be grafted into a framework (e.g., human framework) to provide an antibody molecule.
  • the framework regions can be derived from human germline gene sequences, or be non-germline in origin.
  • VH and/or VL framework residues can be modified as discussed and exemplified herein e.g., using site-directed mutagenesis.
  • Amino acid changes can be made in one or more framework regions and/or one or more CDRs derived from an antibody IL-6a targeted to site II of IL-6 (termed herein a “reference IL-6 antibody”) using methods and parameters known in the art.
  • a resulting IL-6 antagonist that retains binding to site II of an IL-6 (e.g., site II of a human IL-6) and typically has at least the same binding or increased affinity compared to the reference IL-6 antibody.
  • a change that results in a decrease in binding affinity of the derived IL-6a compared to the reference IL-6a e.g., the reference antibody
  • a reference antibody may be an antibody that does not specifically bind an IL-6.
  • a change in the amino acid sequence of an IL-6a polypeptide can include substituting one or more amino acid residue(s) with a non-naturally occurring or non-standard amino acid, modifying one or more amino acid residue into a non-naturally occurring or non-standard form, or inserting one or more non-naturally occurring or non-standard amino acid into the sequence. Examples of numbers and locations of alterations in sequences of the invention are described elsewhere herein.
  • Naturally occurring amino acids include the 20 “standard” L-amino acids identified as G, A, V, L, I, M, P, F, W, S, T, N, Q, Y, C, K, R, H, D, E by their standard single-letter codes.
  • Non-standard amino acids include any other residue that may be incorporated into a polypeptide backbone or result from modification of an existing amino acid residue.
  • Non-standard amino acids may be naturally occurring or non-naturally occurring.
  • Several naturally occurring non-standard amino acids are known in the art, such as 4-hydroxyproline, 5-hydroxylysine, 3-methylhistidine, and N-acetylserine.
  • Those amino acid residues that are derivatized at their N-alpha position will only be located at the N-terminus of an amino-acid sequence.
  • the amino acid is typically an L-amino acid. In some cases the amino acid is a D-amino acid. Alteration may therefore comprise modifying an L-amino acid into, or replacing it with, a D-amino acid.
  • Methylated, acetylated and/or phosphorylated forms of amino acids are also known, and amino acids in the present invention may be subject to such modification.
  • Amino acid sequences in antibody domains and binding members of the invention can comprise non-natural or non-standard amino acids as discussed herein.
  • Non-standard amino acids e.g., D-amino acids
  • D-amino acids can be incorporated into an amino acid sequence using methods known in the art, for example in synthesis of the molecule or by post-synthesis modification or replacement of an amino acid.
  • a D-amino acid is used to increase PK of an IL-6a.
  • Novel VH or VL regions carrying CDR-derived sequences of the invention may be generated using random mutagenesis of one or more selected VH and/or VL nucleic acid sequences to generate mutations within the entire variable domain.
  • error-prone PCR can be used (Chao et al., Nature Protocols, 1:755-768 (2006)).
  • one or two amino acid substitutions are made within an entire variable domain or set of CDRs.
  • Other methods know in the art can be used to generate mutations, for example site-directed mutagenesis, typically in one or more CDRs.
  • One method for producing an antibody IL-6a is to alter a VH domain such as those disclosed herein by adding, deleting, substituting or inserting one or more amino acids.
  • the altered VH domain can be combined with a VL domain (e.g., a VL domain disclosed herein), which can also be altered as described herein and using methods known in the art.
  • VL domain e.g., a VL domain disclosed herein
  • Such altered molecules are tested for their ability to bind to site II of IL-6 and optionally for other desired properties such as increased affinity compared to a reference molecule.
  • a variant VH or VL domain can have 1, 2, 3, 4, or 5 such alterations (e.g., 1, 2, 3, 4, or 5 amino acid substitutions).
  • an IL-6a of the invention is a fragment of an antibody that binds to site II of an IL-6 and comprises an antigen binding site, e.g., can bind to site II of an IL-6.
  • Antibody fragments of the invention are generally obtained starting with a reference (parent) antibody molecule, such as an antibody molecule comprising SEQ ID NO:13 and SEQ ID NO:14.
  • Antibody fragments can be generated using methods known in the art such as recombinant DNA, enzymatic cleavage (for example, using pepsin or papain), chemical cleavage of an antibody (for example, chemical reduction of disulfide bridges).
  • Antibody fragments that comprise an antibody antigen-binding site include, but are not limited to, molecules such as Fab, Fab′, Fab′-SH, scFv, Fv, dAb, Fd, and disulfide stabilized variable region (dsFv).
  • Various other antibody molecules including one or more antibody antigen-binding sites can be engineered, including for example F(ab′)2, F(ab)3, diabodies, triabodies, tetrabodies, and minibodies. Examples of antibody molecules and methods for their construction and use are described in Holliger and Hudson, 2005, Nat Biotechnol 23:1126-1136.
  • Non-limiting examples of binding fragments are a Fab fragment composed of VL, VH, constant light chain domain (CL) and constant heavy chain domain 1 (CH1) domains; an Fd fragment composed of VH and CH1 domains; an Fv fragment composed of the VL and VH domains of a single antibody; a dAb fragment composed of a VH or a VL domain; isolated CDR regions; an F(ab′)2 fragment, a bivalent fragment comprising two linked Fab fragments; a single chain Fv molecule (scFv), in which a VH domain and a VL domain are linked by a peptide linker which allows the two domains to associate to form an antigen binding site; a bispecific single chain Fv dimer (for example as disclosed in WO 1993/011161) and a diabody, which is a multivalent or multispecific fragment constructed using gene fusion (for example as disclosed in WO94/13804).
  • a bispecific single chain Fv dimer for example as disclosed in WO 1993
  • Fv, scFv, or diabody molecules can be stabilized by the incorporation of disulfide bridges linking the VH and VL domains.
  • Minibodies comprising an scFv joined to a CH3 domain can also be used as an IL-6a.
  • Other fragments and derivatives of an antibody that can be used as an IL-6a include a Fab′, which differs from a Fab fragment by the addition of a few amino acid residues at the carboxyl terminus of the heavy chain CH1 domain, including one or more cysteines from the antibody hinge region, and Fab′-SH, which is a Fab′ fragment in which the cysteine residue(s) of the constant domains bear a free thiol group.
  • an IL-6a that is an antibody fragment has been chemically modified to improve or introduce a desirable property, for example PEGylation to increase half-life or incorporation.
  • a dAb domain antibody is a small monomeric antigen-binding fragment of an antibody (the variable region of an antibody heavy or light chain.
  • VH dAbs occur naturally in camelids (e.g., camels and llamas) and can be produced by immunizing a camelid with a target antigen, isolating antigen-specific B cells and directly cloning dAb genes from individual B cells.
  • An IL-6a of the present invention can be a dAb comprising a VH or VL domain substantially as set out herein, or a VH or VL domain comprising a set of CDRs substantially as set out herein.
  • Antibodies of the invention include bispecific antibodies in which two different variable regions are combined in the same molecule.
  • An Il-6a can be incorporated as part of a bispecific antibody prepared using methods known in the art, for example, prepared chemically or from hybrid hybridomas.
  • Such a molecule can be a bispecific antibody fragment of a type discussed above.
  • One non-limiting example of a method for generating a bispecific antibody is BiTETM technology in which the binding domains of two antibodies with different specificity can be used and directly linked via short flexible peptides. This combines two antibodies on a short single polypeptide chain.
  • Diabodies and scFv can be constructed without an Fc region, using only variable domains, potentially reducing the effects of anti-idiotypic reaction.
  • Bispecific antibodies can be constructed as entire IgG, as bispecific Fab′2, as Fab′PEG, as diabodies or else as bispecific scFv. Further, two bispecific antibodies can be linked using routine methods known in the art to form tetravalent antibodies.
  • Bispecific diabodies as opposed to bispecific whole antibodies, are useful, in part because they can be constructed and expressed in E. coli .
  • Diabodies (and many other polypeptides, such as antibody fragments) of appropriate binding specificities can be readily selected using phage display (WO 1994/13804) from libraries. If one arm of the diabody is to be kept constant, for example, with a specificity directed against site II of IL-6, then a library can be made where the other arm is varied and an antibody of appropriate specificity selected.
  • Bispecific whole antibodies may be made by alternative engineering methods as described in described in WO 1996/27011, WO 1998/50431 and WO 2006/028936.
  • an IL-6a of the invention comprises an antigen-binding site within a non-antibody molecule, for example, by incorporating one or more CDRs, e.g. a set of CDRs, in a non-antibody protein scaffold, as discussed further below.
  • the CDRs are incorporated into a non-antibody scaffold.
  • An IL-6 site II binding site can be provided by an arrangement of CDRs on non-antibody protein scaffolds, such as fibronectin or cytochrome B, or by randomizing or mutating amino acid residues of a loop within a protein scaffold to confer binding specificity for an IL-6 site II. Scaffolds for engineering novel binding sites in proteins are known in the art.
  • protein scaffolds for antibody mimics are disclosed in WO200034784, which describes proteins (antibody mimics) that include a fibronectin type III domain having at least one randomized loop.
  • a suitable scaffold into which to graft one or more CDRs, e.g., a set of HCDRs, can be provided by any domain member of the immunoglobulin gene superfamily.
  • the scaffold can be a human or non-human protein.
  • Small size of a binding member may confer useful physiological properties, such as an ability to enter cells, penetrate deep into tissues or reach targets within other structures, or to bind within protein cavities of the target antigen.
  • Typical are proteins having a stable backbone and one or more variable loops, in which the amino acid sequence of the loop or loops is specifically or randomly mutated to create an antigen-binding site that binds the target antigen.
  • proteins include the IgG-binding domains of protein A from S. aureus , transferrin, tetranectin, fibronectin (e.g., using the 10th fibronectin type III domain), lipocalins as well as gamma-crystalline and other AffilinTM scaffolds (Scil Proteins, Halle, Germany).
  • microbodies based on cyclotides—small proteins having intra-molecular disulfide bonds, microproteins (e.g., VersabodiesTM, Amunix Inc., Mountain View, Calif.) and ankyrin repeat proteins (DARPins, e.g., from Molecular Partners AG, Zurich-Schlieren, Switzerland).
  • Such proteins also include small, engineered protein domains such as, for example, immuno-domains (see for example, U.S. Patent Publication Nos. 2003/082630 and 2003/157561).
  • Immuno-domains contain at least one complementarity determining region (CDR) of an antibody.
  • CDR complementarity determining region
  • An IL-6a can comprise additional amino acids, e.g., to impart to the molecule another functional characteristic in addition to ability to bind antigen.
  • an IL-6a carries a detectable label, or is conjugated to a toxin or a targeting moiety or enzyme (e.g., via a peptidyl bond or linker).
  • an IL-6a can comprise a catalytic site (e.g., in an enzyme domain) as well as an antigen binding site (e.g., binding site for site II of an IL-6), such that the antigen binding site binds to the antigen and thus targets the catalytic site to IL-6 or IL-6/IL-6R complex.
  • the catalytic site can, in some cases, further inhibit a biological function of an IL-6, e.g., by cleavage of the IL-6, IL-6R, or other molecule that is associated with the IL-6a/IL-6 complex.
  • the invention includes an antibody IL-6a that has been modified compared to a reference antibody to alter, for example, increase, decrease, or eliminate, the biological effect function of the IL-6a.
  • the Fc region is modified or the parental Fc domain is replaced with a modified Fc domain to alter the pharmacokinetics of the modified IL-6a compared to the unmodified parent.
  • the IL-6a is engineered to have an IgG2 framework.
  • the IL-6a is in an IgG1 or IgG2 framework and has a modified Fc that increases the binding affinity of the IL-6a at pH 6.0 and does not substantially alter the binding affinity at pH 7.0 compared to a parent or other reference IL-6a.
  • the Fc domain is modified and the IL-6a has reduced systemic accumulation, a decreased half-life, and/or increased systemic clearance compared to a parent or other reference IL-6a.
  • an antibody IL-6a is modified to increase complement fixation and complement-dependent cytotoxicity.
  • the antibody IL-6a is modified to increase the ability of the antibody compared to a reference antibody to activate effector cells and participate in antibody-dependent cytotoxicity (ADCC).
  • the antibodies as disclosed herein can be modified both to enhance their capability of activating effector cells and participating in antibody-dependent cytotoxicity (ADCC) and to enhance their capability of fixing complement and participating in complement-dependent cytotoxicity (CDC).
  • the antibodies disclosed herein are modified to reduce their ability to fix complement and participate in complement-dependent cytotoxicity (CDC). In other embodiments, the antibodies are modified to reduce their ability to activate effector cells and participate in antibody-dependent cytotoxicity (ADCC). In yet other embodiments, an antibody as disclosed herein can be modified both to reduce its ability to activate effector cells and participate in antibody-dependent cytotoxicity (ADCC) and to reduce its ability to fix complement and participate in complement-dependent cytotoxicity (CDC).
  • the formulation described herein includes an IL-6 antagonist, e.g., an IL-6 antibody or fragment thereof, present in the formulation in a concentration of from 0.1 mg/ml to 100 mg/ml, 0.1-80 mg/ml, 0.1 to 50 mg/ml, 0.1 mg/ml to 20 mg/ml, 0.1 mg/ml to 5 mg/ml, 0.1 mg/ml to 1 mg/ml, 1 mg/ml to 100 mg/ml; 5 mg/ml to 100 mg/ml; 5 mg/ml to 30 mg/ml; 10 mg/ml to 100 mg/ml; 10 mg/ml to 30 mg/ml; 20 mg/ml to 100 mg/ml; 30 mg/ml to 100 mg/ml; 40 mg/ml to 100 mg/ml; 50 mg/ml to 100 mg/ml; 60 mg/ml to 100 mg/ml; 1 mg/ml to 80 mg/ml; 5 mg/ml to 80 mg/ml; 10 mg/
  • the formulation contains about 1 mg/ml, 2 mg/ml, 5 mg/ml, 10 mg/ml, 20 mg/ml, 25 mg/ml, 30 mg/ml, 40 mg/ml, 50 mg/ml, or 55 mg/ml of an IL-6 antagonist, e.g., an IL-6 antibody or fragment described herein.
  • an IL-6 antagonist e.g., an IL-6 antibody or fragment described herein.
  • the IL-6 antagonist e.g., IL-6 antibody or fragment thereof described herein, is formulated with other pharmaceutically effective excipients.
  • the IL-6 antagonist e.g., IL-6 antibody or fragment thereof described herein, is formulated with one or more, or all of the following: a buffer, a surfactant, and a tonicity agent (e.g., a sugar and/or a salt).
  • the formulation comprises an IL-6a, e.g., an IL-6 antibody or fragment thereof as described herein, and one or more buffers (e.g., buffering agents).
  • the formulation further comprises one or more surfactants.
  • the formulation further comprises one or more tonicity agents.
  • the formulation comprises two or more tonicity agents, e.g., a salt and a sugar.
  • the formulation further comprises one or more of a chelating agent, one or more of a preserving agent, one or more of an antioxidant, and/or one or more of an amino acid.
  • the formulation further comprises a one or more additional therapeutic agents, e.g., a second therapeutic agent. Exemplary excipients and additional therapeutic agents are described further herein.
  • a suitable buffer is selected by conducting a stability study in which the polypeptide of interest, e.g., an IL-6a, e.g., an IL-6 antagonist or fragment thereof, is exposed to various buffers at various pH's, concentrations, temperatures, and for various times.
  • the polypeptide of interest e.g., an IL-6a, e.g., an IL-6 antagonist or fragment thereof.
  • Buffers can be selected, for example by placing the polypeptide of interest, e.g., an IL-6a, e.g., an IL-6 antibody or fragment thereof, in the buffer and subjecting the samples to elevated temperatures (accelerated stability testing) then test for physical stability (precipitation by visual inspection) or chemical stability, for example, by monitoring deamidation by weak cation exchange chromatography or oxidation by reversed phase chromatography. Additional assays can include monitoring of A280, SDS-PAGE, pH, and osmolality.
  • a buffer that provides the best physical and chemical stability is selected.
  • the buffer provides the liquid composition with the desired pH close also provides enhanced antibody stability and resistance to aggregation, oxidation and fragmentation.
  • buffering agents include, but are not limited to, acetate, succinate, gluconate, citrate, histidine, acetic acid, phosphate, phosphoric acid, ascorbate, tartartic acid, maleic acid, glycine, lactate, lactic acid, ascorbic acid, imidazole, bicarbonate and carbonic acid, succinic acid, sodium benzoate, benzoic acid, gluconate, edetate, acetate, malate, imidazole, tris (tricine), phosphate, and mixtures thereof.
  • the buffering agent is selected from the group consisting of acetate, citrate, histine, phosphate, and tris (tricine).
  • the buffering agent is present in an amount of from about 1 mM to about 50 mM, from about 5 mM to about 40 mM, from about 5 mM to about 30 mM, from about 5 mM to about 20 mM, from about 10 mM to about 50 mM, from about 10 mM to about 40 mM, from about 10 mM to about 30 mM, from about 15 mM to about 50 mM, from about 15 mM to about 40 mM, from about 15 mM to about 30 mM, from about 15 mM to about 25 mM, from about 18 mM to about 22 mM.
  • the buffering agent is present in an amount of about 10 mM, about 15 mM, about 16 mM, about 17 mM, about 18 mM, about 19 mM, about 20 mM, about 21 mM, about 22 mM, about 23 mM, about 24 mM, about 25 mM, about 30 mM, about 35 mM, about 40 mM, about 45 mM or about 50 mM.
  • the formulation comprises concentration of a buffer, e.g., a histidine buffer, which is between at least 10% less than and at least 10% greater than a concentration of a buffer disclosed herein.
  • the formulation comprises 20 mM+/ ⁇ 10% of buffer.
  • the formulation further comprises 20 mM+/ ⁇ 20% buffer.
  • the formulation further comprises 20 mM+/ ⁇ 30% buffer.
  • the buffering agent is histidine, wherein the histidine can comprise either L-histidine or D-histidine, a solvated form of histidine, a hydrated form (e.g., monohydrate) of histidine, or an anhydrous form of histidine or a mixture thereof.
  • the buffering agent is histidine hydrochloride (HCl).
  • the histidine buffer is present at a concentration of about
  • the formulations provided herein include one or more surfactants.
  • a surfactant can be useful, e.g., for reducing adhesion of a molecule to a container, reducing aggregation of a protein particularly under conditions of agitation.
  • Suitable surfactants and concentrations of such surfactants can be determined by testing whether the surfactant prevents aggregation in agitation studies. Methods of conducting such studies are known in the art. For example, it can be determined whether surfactant is needed to prevent precipitation from agitation stress. In such experiments, typically, a screen is performed using agitation and analysis.
  • concentrations used for such studies are 0.01%, 0.02%, 0.06%, and 0.1% w/v surfactant, e.g., poloxamer 188.
  • aggregation and/or precipitation are assessed using analysis by spectrophotometry (A280), visual inspection, size exclusion chromatography (SEC), light obscuration (e.g., using a HIAC device), or Micro-Flow ImagingTM (MFI, ProteinSimple, Santa Clara, Calif.).
  • a surfactant is generally selected for use in a formulation that is associated with the least amount of precipitation, e.g., no visible precipitation, or particle count that meets guidelines for particulate matter in injections (see, e.g., USP ⁇ 788>) or guidelines for particulate matter in ophthalmic solutions (see, e.g., USP ⁇ 789>).
  • Surfactants suitable for use in the disclosed formulations can include, but are not limited to: polysorbates (e.g., polysorbate 20, polysorbate 21, polysorbate 40, polysorbate 60, polysorbate 61, polysorbate 65, polysorbate 80, polysorbate 81, polysorbate 85, and mixtures thereof), poloxamers (e.g., poloxamer P188), tritons (e.g., Triton X100 or Triton X405), sodium dodecyl sulfate, sodium laurel sulfate, sodium octyl glycoside, lauryl-sulfobetaine, myhstyl-sulfobetaine, linoleyl-sulfobetaine, stearyl-sulfobetaine, lauryl-sarcosine, myristyl-sarcosine, linoleyl-sarcosine, stearyl-sarcosine, linoleyl
  • a formulation contains a surfactant (e.g., polysorbate 20 or Tween 20) in a concentration ranging from about 0.001% to about 10%, from about 0.005% to about 5%, from about 0.01% to about 1%, from about 0.05% to about 0.5%, from about 0.05% to about 0.1%, from about 0.01% to about 0.8%, from about 0.01% to about 0.05%, from about 0.02% to about 0.08%, from about 0.02% to about 0.05%, or from about 0.02% to about 0.05% w/v.
  • a surfactant e.g., polysorbate 20 or Tween 20
  • the formulation contains a surfactant at a concentration of about 0.01%, about 0.02%, about 0.03%, about 0.04%, about 0.05%, about 0.06%, about 0.07%, about 0.08%, about 0.09%, about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5% or about 1% w/v.
  • the formulation comprises concentration of a surfactant, which is between at least 10% less than and at least 10% greater than a concentration of a surfactant disclosed herein.
  • the formulation comprises 0.03%+/ ⁇ 0.003% of surfactant.
  • the formulation further comprises 0.03%+/ ⁇ 0.006% of surfactant.
  • the formulation further comprises 0.03%+/ ⁇ 0.01% of surfactant.
  • the surfactant is polysorbate 20 (e.g., Tween 20), polysorbate 80 (Tween 80), or poloaxamer 188.
  • polysorbate 20, polysorbate-80, or poloxamer 188 is present in the formulation at a concentration ranging from about 0.01% to about 0.2%, about 0.01% to about 0.1%, 0.01% to about 0.05%, 0.01% to about 0.03%, 0.02% to about 0.8%, 0.02%, to about 0.05%, 0.02% to about 0.04% w/v.
  • polysorbate 20, polysorbate-80, or poloxamer 188 is present in the formulation at a concentration of about 0.01%, 0.02%, 0.03%, 0.04%, or 0.05% w/v, e.g., at a concentration of 0.03% w/v.
  • polysorbate-20 (Tween-20), polysorbate-80 (Tween-80), and poloxamer 188 are interchangeable.
  • the formulations described herein include one or more tonicity agents.
  • a formulation described herein may contain two tonicity agents.
  • Tonicity agents refers to an excipient that can adjust the osmotic pressure of a formulation to isotonic to that the formulation is physiologically compatible with the cells of the of the body tissue or organ of the subject.
  • a tonicity agent can be a polyol such as a sugar (e.g., a saccharide), a carbohydrate, a salt, or mixtures thereof.
  • a sugar e.g., a saccharide
  • a carbohydrate e.g., a salt, or mixtures thereof.
  • such agents may contribute to the stability of an IL-6 antagonist, e.g., an IL-6 antibody or fragment thereof as described herein.
  • the tonicity agent is a polyol.
  • Polyols refers to an excipient with multiple hydroxyl groups, and includes sugars (reducing and non-reducing sugars, sugar alcohols, and sugar acids.
  • the polyol has a molecular weight that is less than about 600 kD (e.g., in the range from about 120 to about 400 kD).
  • Suitable polyols include, but are not limited to, mannitol, trehalose, sorbitol, erythritol, isomalt, lactitol, maltitol, xylitol, glycerol, lactitol, propylene glycol, polyethylene glycol, inositol, or mixtures thereof.
  • suitable sugars and carbohydrates include monosaccharides, disaccharides and polysaccharides or mixtures thereof.
  • Suitable sugars e.g., saccharides
  • carbohydrates include, but are not limited to, fructose, glucose, mannose, sucrose, sorbose, xylose, lactose, maltose, sucrose, dextran, pullulan, dextrin, cyclodextrins, soluble starch, hydroxyethyl starch, water-soluble glucans, and mixtures thereof.
  • the formulation includes a polyol, a sugar, or a carbohydrate as described herein at a concentration ranging from between about 0.1% to about 20%, from about 1% to about 10%, from about 1% to about 8%, from about 1% to about 5%, from about 2% to about 10%, from about 2% to about 8%, from about 2% to about 5%, from about 3% to about 10%, from about 3 to about 8%, from about 3% to about 5%.
  • the formulation contains a polyol, a sugar, or a carbohydrate at a concentration of about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%, about 9%, or about 10%.
  • the formulation comprises concentration of polyol, which is between at least 10% less than and at least 10% greater than a concentration of a polyol disclosed herein.
  • concentration of polyol which is between at least 10% less than and at least 10% greater than a concentration of a polyol disclosed herein.
  • the formulation comprises 4%+/ ⁇ 0.4% polyol.
  • the formulation further comprises 4%+/ ⁇ 0.8% sorbitol.
  • the formulation further comprises 4%+/ ⁇ 1.2% polyol.
  • the formulation includes a tonicity agent, wherein the tonicity agent is selected from sorbitol or trehalose. In one embodiment, the formulation contains sorbital. In one embodiment, the concentration of sorbitol in the formulation is between about 1% to about 10%, between about 1% to about 5%, between about 2% to about 5%, or about 4%.
  • the tonicity agent is a salt.
  • salts e.g., sodium chloride
  • inclusion of salts may also improve antibody stability by protecting the protein from deamidation.
  • Suitable salts include, but are not limited to, sodium chloride, sodium succinate, sodium sulfate, potassium chloride, magnesium chloride, magnesium sulfate, and calcium chloride.
  • the formulation includes a salt at a concentration ranging from about 1 mM to about 200 mM, from about 10 mM to about 150 mM, from about 20 mM to about 150 mM, from about 50 mM to about 150 mM, from about 100 mM to about 150 mM, from about 10 mM to about 100 mM, from about 10 mM to about 50 mM, from about 10 mM to about 40 mM, from about 10 mM to about 30 mM, from about 15 mM to about 50 mM, from about 15 mM to about 30 mM, or from about 15 mM to about 25 mM.
  • the formulation contains a salt at a concentration of about 10 mM, 15 mM, 20 mM, 25 mM, 50 mM, or 150 mM, e.g., 20 mM.
  • the formulation comprises concentration of salt, which is between at least 10% less than and at least 10% greater than a concentration of a salt disclosed herein.
  • the formulation comprises 20 mM+/ ⁇ 10% of salt.
  • the formulation further comprises 20 mM+/ ⁇ 20% salt.
  • the formulation further comprises 20 mM+/ ⁇ 30% salt.
  • the formulation contains a salt, wherein the salt is sodium chloride.
  • the formulation comprises sodium chloride at a concentration of from about 10 mM to about 150 mM, from about 10 mM to about 50 mM, from about 10 mM to about 30 mM, from about 15 mM to about 25 mM, or about 10 mM, about 15 mM, about 20 mM, or about 25 mM.
  • the formulation includes two tonicity agents, e.g., a polyol (a sugar) and a salt.
  • the formulation includes sorbitol and sodium chloride.
  • the formulations provided herein are isotonic for the eye (e.g., having an osmolality of about 270-330 mOsm per kg). In some embodiments, the formulation has an osmolality of from about 250 to about 450 mOsm per kg, 300 to 400 mOsm per kg, 350 to 400 mOsm per kg, 200 to 375 mOsm per kg, or 350 to 375 mOsm per kg. In embodiments, the formulation has an osmolality of 270-330 mOsm per kg, e.g., about 320 mOsm per kg.
  • the formulations featured in the invention may also contain other pharmaceutically acceptable excipients. See e.g., Gennaro (ed.), Remington: The Science and Practice of Pharmacy, 20th ed., Lippincott, Williams & Wilkins (2000) (ISBN: 0683306472); Ansel et al., Pharmaceutical Dosage Forms and Drug Delivery Systems, 7th Ed., Lippincott Williams & Wilkins Publishers (1999) (ISBN: 0683305727); Kibbe (ed.), Handbook of Pharmaceutical Excipients, 3rd ed. (2000) (ISBN: 091733096X); Protein Formulation and Delivery, McNally and Hastedt (eds.), Informa Health Care (ISBN: 0849379490) (2007).
  • excipients that can be added are chelating agents, amino acids, preservatives (e.g., preserving agents), penetration enhancers, bioadhesives, stabilizers, antioxidants, and viscosity agents.
  • the formulation can include one or more penetration enhancer and/or bioadhesive.
  • Penetration enhancers and bioadhesives may include, for example, chitosan, cytochalasin B, aminated gelatin, poly- ⁇ -caprolectone (carbopol 941P); poly(butylcyanoacrylate); poly-L-arginine; cyclodextrins; gellan; poly(acrylic acid); hyaluronic acid; mucin; alginate; a carbophil, and poloxamers (e.g., see Nagarwal et al., J Controlled Release, 136:2-13 (2009); Ding, PSTT 1:328-35 (1998); and Sahoo et al., Drug Discovery Today, 13:144-51 (2008).
  • excipients may be useful as stabilizers, and can include, for example, glycerin, potassium chloride, potassium phosphate, propylene glycol, sodium acetate, sodium bisulfite, sodium borate, sodium borate decahydrate, sodium chloride, sodium citrate, sodium phosphate, sodium phosphate (including sodium phosphate monobasic and dibasic); zinc chloride, phenol, benzoate, derivatives of castor oil and ethylene oxides, and Cremophor® (BASF Corp., Germany).
  • glycerin potassium chloride, potassium phosphate, propylene glycol, sodium acetate, sodium bisulfite, sodium borate, sodium borate decahydrate, sodium chloride, sodium citrate, sodium phosphate, sodium phosphate (including sodium phosphate monobasic and dibasic); zinc chloride, phenol, benzoate, derivatives of castor oil and ethylene oxides, and Cremophor® (BASF Corp., Germany).
  • the formulation can include one or more chelating agents.
  • Chelating agents can lower the formation of reduced oxygen species, reduce acidic species (e.g., deamidation) formation, reduce antibody aggregation, and/or reduce antibody fragmentation, and/or reduce antibody oxidation in the compositions of the present invention.
  • Such chelating agents can reduce or prevent degradation of an antibody that is formulated in comparison to the antibody without the protection of a chelating agent.
  • Suitable chelating agent include, but are not limited to aminopolycarboxylic acids, hydroxyaminocarboxylic acids, N-substituted glycines, 2-(2-amino-2-oxocthyl) aminoethane sulfonic acid (BES), deferoxamine (DEF), citric acid, niacinamide, and desoxycholates and mixtures thereof.
  • the chelating agent is selected from the group consisting of ethylenediaminetetraacetic acid (EDTA), diethylenetriamine pentaacetic acid 5 (DTPA), nithlothacetic acid (NTA), N-2-acetamido-2-iminodiacetic acid (ADA), bis(aminoethyl)glycolether, N,N,1ST,IST-tetraacetic acid (EGTA), trans-diaminocyclohexane tetraacetic acid (DCTA), glutamic acid, and aspartic acid, N-hydroxyethyliminodiacetic acid (HIMDA), N, N-bis-hydroxyethylglycine (bicine) and N-(thshydroxymethylmethyl) 10 glycine (tricine), glycylglycine, sodium desoxycholate, ethylenediamine; propylenediamine; diethylenetriamine; triethylenetetraamine (trien), ethylenediaminetetraaceto
  • the chelating agent is selected from the group consisting of salts of EDTA including dipotassium edetate, disodium edetate, edetate calcium disodium, sodium edetate, trisodium edetate, and potassium edetate; and a suitable salt of deferoxamine (DEF) is deferoxamine mesylate (DFM), or mixtures thereof.
  • DEF deferoxamine
  • DMF deferoxamine mesylate
  • Chelating agents used in the invention can be present, where possible, as the free acid or free base form or salt form of the compound, also as an anhydrous, solvated or hydrated form of the compound or corresponding salt.
  • the formulation can include one or more amino acids. Suitable amino acids include, but are not limited to: arginine, glutamic acid, histidine, or methionine.
  • the amino acid is typically selected to enhance the stability and/or the solubility of the protein. Methods of identifying such amino acids are known in the art.
  • a formulation contains arginine.
  • the formulation can include one or more viscosity agents. Viscocity agents are generally included in ophthalmic formulations to increase the residence time of an ophthalmic treatment that would otherwise be rapidly cleared by blinking and drainage through the conjunctival sac.
  • Suitable viscosity agents include, but are not limited to, methylcelluloses, including sodium carboxymethyl cellulose (also referred to herein as carboxymethyl cellulose or CMC); hydroxy celluloses, including ethyl cellulose; hydroxypropyl methylcellulose (hypromellose); carbomers, such as 934P, 971P and 974P; polyvinyl alcohol; xanthan gum; guar gum; gellan gum; and glycerin.
  • the formulation can include one or more antioxidants. Suitable antioxidants include, but are not limited to, methionine, sodium thiosulfate, catalase, and platinum.
  • the formulation can include one or more preservatives, e.g., to prevent microbial and fungal contamination during use, and/or one or more detergents, or surfactants, e.g., to solubilize proteins.
  • Suitable preservatives include, but are not limited to: benzalkonium chloride, benzalthonium chloride, benzyl alcohol, chlorobutanol, benzododecinium bromide, methyl paraben, propyl paraben, phenylethyl alcohol, phenoxyethanol, phenol, m-cresol, edetate disodium, sorbic acid, and polyquaternium-1, and can be included at a concentration of from 0.001 w/v to 1.0% w/v.
  • a formulation containing a therapeutic protein as described herein is sterile yet free of preservatives.
  • the formulation can also include other compounds that act as a lubricant or wetting agent.
  • viscosity agents such as: monomeric polyols, such as, glycerol, propylene glycol, ethylene glycol; polymeric polyols, such as polyethylene glycol, various polymers of the cellulose family: hydroxypropylmethyl cellulose (“HPMC”), sodium carboxymethyl cellulose, hydroxy propylcellulose (“HPC”), dextrans, such as dextran 70; water soluble proteins, such as gelatin; and vinyl polymers, such as polyvinyl alcohol, polyvinylpyrrolidone, povidone and carbomers, such as carbomer 934P, carbomer 941; carbomer 940, carbomer 974P.
  • HPMC hydroxypropylmethyl cellulose
  • HPC hydroxy propylcellulose
  • dextrans such as dextran 70
  • water soluble proteins such as gelatin
  • vinyl polymers such as polyvinyl alcohol, polyvinylpyrrol
  • Still additional examples include polysaccharides, such as hyaluronic acid and its salts and chondroitin sulfate and its salts, and acrylic acid polymers.
  • the formulation has a viscosity between 1 cP to 400 cP.
  • the formulations provided herein has a pH between about 5.0 to about 7.5, about 5.5 to about 7.5, about 6.0 to about 7.5, about 6.5 to about 7.5, about 5.0 to about 7.0, about 5.5 to about 7.0, about 6.0 to about 7.0, or about 6.2 to about 6.8.
  • the formulations provided herein have a pH of less than 7.5, less than 7.0, less than 6.9, less than 6.8, less than 6.6, less than 6.5, less than 6.4, less than 6.3, less than 6.2, or less than 6.0.
  • the formulation provided herein has a pH of about 6.5, or 6.5.
  • pH levels of a composition or formulation described herein can be adjusted during the formulation process in any of the methods known in the art.
  • highly concentrated acid e.g., hydrogen chloride (HCl) or highly concentrated base, e.g., sodium hydroxide (NaOH)
  • HCl hydrogen chloride
  • NaOH sodium hydroxide
  • proteins e.g., antibodies
  • Agitation stress can lead to precipitation and heat stress can lead to precipitation and to chemical degradation.
  • heat stress can be exposure to heat stress. Applicants have achieved a formulation that successfully provides excellent stability when exposed to agitation stress and heat.
  • a formulation described herein is stable.
  • the formulation exhibits stability under conditions (e.g., storage at particular temperatures, or agitation stress) described herein.
  • stability is assessed using one or more methods described herein (e.g., based on visual appearance, content by spectrophotometry (A280), SDS-PAGE non-reduced, SDS-PAGE reduced; size exclusion HPLC (SE HPLC) or SE-UPLC; reverse phase HPLC (RP-HPLC); weak anion exchange HPLC (WAEX-HPLC); potency; a light obscuration particle count test (e.g., a light obscuration particle count test as described in USP ⁇ 789>); or a microscopic particle count test (e.g., a microscopic particle count test as described in USP ⁇ 789>)) and/or methods known in the art.
  • A280 visual appearance, content by spectrophotometry
  • SE HPLC size exclusion HPLC
  • RP-HPLC reverse phase HPLC
  • Stability can be assessed based on visual appearance.
  • a formulation is stable if it is a clear to slightly opalescent colorless solution essentially free from visible particulates.
  • the formulation is stable at about 25° C. to about 40° C., for example, about 27° C., about 28° C., about 29° C., about 30° C., about 31° C., about 32° C., about 33° C., about 34° C., about 35° C., about 36° C., about 37° C., about 38° C., about 39° C., or about 40° C.
  • the formulations are stable for long periods of time during storage at temperatures of from about 2° C. to about 8° C., such as at about 4° C., about 5° C., about 6° C., from 2° C. to 8° C., at 4° C., at 5° C., or at 6° C.
  • the formulations are stable at such storage temperatures for a period of at least two weeks; four weeks; six weeks; two months; three months; six months, one year, two years, three years, or four years.
  • Stability of a formulation can be assessed, e.g., after storage for at least 2, 4, 6, 8, 12, or 18 months, e.g., at 2-8° C., or after storage under ambient conditions, e.g., at room temperature (RT), e.g. at about 25° C. for, e.g., at least 2 weeks, 1 month, 2 months, 3 months 5 months, 6 months, 12 months, or 18 months.
  • RT room temperature
  • the formulation is stable after storage at 2-8° C. for at least 8 months.
  • the formulation is stable after exposure to room temperature for at least 5 months.
  • the formulation is stable after storage, e.g., for at least 5 months, in a BFS container.
  • Stability can be assessed, e.g., based on methods and criteria described herein or known in the art. For example, stability can be assessed based on physical purity (e.g., lack of aggregation, e.g., as assessed using size exclusion HPLC, also referred to herein as size exclusion, SE HPLC, or SEC HPLC), chemical purity (e.g., as assessed using weak anion exchange HPLC, reverse phase HPLC, and/or SDS PAGE (e.g., reduced or nonreduced SDS PAGE)), and/or the levels of particulates (e.g., as assessed visually or by particle count using an HIAC liquid particle counter (Beckman Coulter, Brea, Calif.)).
  • physical purity e.g., lack of aggregation, e.g., as assessed using size exclusion HPLC, also referred to herein as size exclusion, SE HPLC, or SEC HPLC
  • chemical purity e.g., as assessed using weak anion exchange HPLC, reverse
  • stability is demonstrated based on compliance with guidelines for particulate matter in opthalmic solutions, e.g., as set forth in USP ⁇ 789> (U.S. Pharmacopeia, Particulate Matter in Opthalmic Solutions).
  • the formulation has less than or equal to 50 particles per ml for particles ⁇ 10 ⁇ m and/or less than or equal to 5 particles per ml for particles ⁇ 25 ⁇ m, e.g., as assessed using a light obscuration particle count test (e.g., a light obscuration particle count test as described in USP ⁇ 789>).
  • a light obscuration particle count test e.g., a light obscuration particle count test as described in USP ⁇ 789>.
  • the formulation has less than or equal to 50 particles per ml for particles ⁇ 10 ⁇ m, less than or equal to 5 particles per ml for particles ⁇ 25 ⁇ m, and/or less than or equal to 2 particles per ml for particles ⁇ 50 ⁇ m, e.g., as assessed using a microscopic particle count test (e.g., a microscopic particle count test as described in USP ⁇ 789>).
  • a microscopic particle count test e.g., a microscopic particle count test as described in USP ⁇ 789>.
  • stability is demonstrated based on compliance with guidelines for particulate matter in injections, e.g., as set forth in USP ⁇ 789> (U.S. Pharmacopeia, Particulate Matter in Injections).
  • the formulation has less than or equal to 6000 particles per container (for containers with a volume of 100 ml or less) for particles ⁇ 10 ⁇ m, and/or less than or equal to 600 particles per container (for containers with a volume of 100 ml or lower) for particles ⁇ 25 um, e.g., as assessed using a light obscuration particle count test (e.g., a light obscuration particle count test as described in USP ⁇ 789>).
  • a light obscuration particle count test e.g., a light obscuration particle count test as described in USP ⁇ 789>.
  • the formulation has less than or equal to 3000 particles per 5 ml for particles ⁇ 10 ⁇ m and/or less than or equal to 300 particles per 5 ml for particles ⁇ 25 um, e.g., as assessed using a microscopic particle count test (e.g., a microscopic particle count test as described in USP ⁇ 789>).
  • a microscopic particle count test e.g., a microscopic particle count test as described in USP ⁇ 789>.
  • the protein in a formulation is protected from agitation stress as demonstrated, e.g., by lack of aggregation (lack of aggregation may be demonstrated, e.g., if the formulation contains >90%, >91%, >92%, >93%, >94%, >95%, >96%, >97%, >98%, or >99% of the monomeric form of the protein relative to aggregated form) after vortexing the protein solution, e.g., for 1-8 hours at room temperature (RT), e.g., for 4 hours at RT.
  • Aggregation can be assessed, e.g., using methods described herein or methods known in the art. For example, aggregation can be assessed using ultracentrifugation, size-exclusion chromatography, gel electrophoresis, dynamic light scattering, and/or turbidity measurements.
  • stability is assayed by physical or chemical methods known in the art. For example, physical purity or lack of aggregation can be determined using size exclusion HPLC or other methods that determine the relative amount of monomeric polypeptide in a formulation.
  • a formulation with acceptable stability contains >90% of the monomeric form of therapeutic protein (e.g., an IL-6a, e.g., an IL-6 antibody or fragment thereof described herein) relative to aggregated forms of the protein.
  • the formulation contains >90% (e.g., >91%, >92%, >93%, >94%, >95%, >96%, >97%, >98%, or >99%) of the monomeric form of the therapeutic protein (e.g., an IL-6a, e.g., an IL-6 antibody or fragment thereof described herein), relative to aggregated forms of the protein.
  • the monomeric form of the therapeutic protein e.g., an IL-6a, e.g., an IL-6 antibody or fragment thereof described herein
  • Chemical purity can be determined, for example, using weak cation exchange HPLC or reverse phase HPLC.
  • a formulation with acceptable stability contains >80% of the native molecule, relative to chemically modified forms of the molecule, e.g., as assessed using weak cation exchange HPLC.
  • the formulation contains >80% (e.g., >85%, >87%, >90%, or >95%) of the native molecule, relative to chemically modified forms of the molecule (e.g., oxidized or acetylated forms).
  • Particulates may be identified visually.
  • the formulation is one that is essentially free of particulates that can be identified visually.
  • Biologic treatments can be problematic to administer because they can have a relatively short shelf life or require special storage conditions that can create obstacles for storage, transport, and patient use as well as assuring a sufficient supply of the biologic.
  • An advantage of certain formulations provided herein is that the formulations are surprisingly stable not only under conditions of refrigeration, but also at temperatures that are in accord with room temperature (e.g., 25° C.) and above (e.g., 40° C.). Accordingly, the cytokine protein or polypeptide formulations (e.g.
  • heterologous cytokine protein or polypeptide formulations are, in some embodiments, provided in a liquid form that is stable at RT (e.g., at 25° C.) for a period of at least three days, five days, one week, ten days, two weeks, three weeks, six weeks, eight weeks, 16 weeks, 20 weeks, 25 weeks, 30 weeks, 35 weeks, 40 weeks, 45 weeks, one month, two months, three months, four months, five months, six months, seven months, eight months, twelve months, or more.
  • a month is determined on date to date basis, e.g., from the first of the month to the first of the second month.
  • the formulations are stable at about 25° C. to about 40° C., for example, about 27° C., about 28° C., about 29° C., about 30° C., about 31° C., about 32° C., about 33° C., about 34° C., about 35° C., about 36° C., about 37° C., about 38° C., about 39° C., or about 40° C.
  • compositions and formulations described herein be formulated in a variety of forms. These include, for example, liquid, semi-solid, and solid dosage forms, such as liquid solutions (e.g., injectable and infusible solutions), dispersions or suspensions, including nanoparticles and liposomes.
  • liquid solutions e.g., injectable and infusible solutions
  • dispersions or suspensions including nanoparticles and liposomes.
  • the form will generally depend on the intended mode of administration and therapeutic application.
  • Compositions for the agents described herein are typically in the form of injectable or infusible solutions, or are formulated for topical delivery, e.g., topical ocular delivery.
  • a pharmaceutical composition described herein is sterile and stable under the conditions of manufacture and storage.
  • a pharmaceutical composition can also be tested to ensure it meets regulatory and industry standards for administration.
  • the composition can be formulated as a solution, microemulsion, dispersion, liposome, or other ordered structure suitable to high drug (e.g., a biologic) concentration.
  • Sterile injectable solutions can be prepared by incorporating an agent described herein in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilization.
  • dispersions are prepared by incorporating an agent described herein into a sterile vehicle that contains a basic dispersion medium and the required other ingredients from those enumerated above.
  • exemplary methods of preparation include vacuum drying and freeze-drying that yields a powder of an agent described herein plus any additional desired ingredient from a previously sterile-filtered solution thereof.
  • the proper fluidity of a solution can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants.
  • Prolonged absorption of injectable compositions can be engineered by inclusion of an agent that delays absorption, for example, monostearate salts and gelatin. Such an agent may be particularly useful in a low-dose formulation.
  • the formulation comprises ⁇ 1 mg/ml of a therapeutic protein (e.g., a an IL-6a, e.g., an IL-6 antibody or fragment thereof described herein) and gelatin is included in the formulation.
  • a formulation is prepared with a carrier.
  • the formulation can be delivered, for example, as a controlled release formulation, delivered by an implant or a microencapsulated delivery system.
  • Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. See e.g., Sustained and Controlled Release Drug Delivery Systems, J. R. Robinson, ed., Marcel Dekker, Inc., New York, 1978.
  • Ophthalmic packs may be used to give prolonged contact of an ophthalmic formulation with the eye.
  • a cotton pledget is saturated with the formulation and then inserted into the superior or inferior fornix.
  • the formulation may also be administered by the way of iontophoresis. This procedure keeps the solution in contact with the cornea in an eyecup bearing an electrode. Diffusion of the drug is effected by difference of electrical potential.
  • Iontophoretic systems which have been used include Ocuphor®1 (Iomed Inc., USA); Eyegate® II Delivery System1 (EyeGate Pharma, USA); and Visulex®1 (Aciont Inc., USA). See Amo and Urtti, Drug Discovery Today, 13:143 (2008).
  • Another strategy for sustained ocular delivery is the use of gelifying agents. These materials can be delivered in a liquid form, as an eye drop or intraocular injection. After instillation the polymer undergoes a phase change and forms a semi-solid or solid matrix that releases the drug over prolonged period. The phase transition can be induced by changes in the temperature, ion concentration, or pH.
  • the gel forming solutions such as Timoptic®-XE1 (Merck and Co. Inc., USA), which contains Gelrite® (purified anionic heteropolysaccharide from gellan gum); Pilogel®1 (Alcon, Inc., Switzerland) eye drops contain poly(acrylic acid); and Azasite®1 (Insite Vision, USA) have been tested clinically. These materials enhance the drug retention relative to the conventional eye drops and lead to increased drug absorption into the eye and reduced dosing frequency. See Amo and Urtti, Drug Discovery Today, 13:135-143 (2008).
  • a formulation featured in the invention can be delivered by injection, e.g., intravitreal, periocular, or subconjunctival injection.
  • the formulation can be injected underneath the conjunctiva facilitating passage through the sclera and into the eye by simple diffusion.
  • the formulation can also be injected underneath the conjunctiva and the underlying Tenon's capsule in the more posterior portion of the eye to deliver the agent to the ciliary body, choroid, and retina.
  • the formulation may also be administered by retrobulbar injection.
  • a formulation described herein can be administered to a subject, by any suitable method, such as intravenous administration as a bolus or by continuous infusion over a period of time, by intramuscular, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, intrasynovial, transtracheal, subcutaneous, subcuticular, intraarticular, subcapsular, subarachnoid, intraspinal, epidural injection, intrasternal injection and infusion.
  • suitable modes of administration include topical (e.g., dermal or mucosal) or inhalation (e.g., intranasal or intrapulmonary) routes.
  • the route of administration is one of: intravenous injection or infusion, subcutaneous injection, or intramuscular injection.
  • the mode of administration for a formulation featured is topical administration to the eye, e.g., in the form of drops.
  • devices that may contain the formulation and/or be used for administration of the formulation include simple eye droppers, squeeze bottles with or without metering function, and blow/fill/seal (BFS) devices such as those manufactured by Catalent (Somerset, N.J.), multiuse devices using, for example tip-seal technology, silver/oligodynamic technology, sterile filters, collapsing primary containers, and the like.
  • the container is suitable to provide a shelf-life of at least two years, e.g., at least 3 years, at least 4 years, or at least 5 years, e.g., at 5° C. In embodiments, the container is suitable to provide a shelf-life of at least 3 years at 5° C. In embodiments, the container is suitable to provide a shelf-life of at least 2 months, 3 months, 4 months, 5 months, 6 months, 8 months, 10 months, or 12 months at RT.
  • the container is suitable to provide a shelf-life of at least 5 months at RT.
  • suitable container materials are known in the art, for example certain plastics, for example, low density polyethylene (LDPE), high density polyethylene (HDPE), or polypropylene.
  • the formulation can be prepared for single use application in a container or can be prepared for use in a multiuse container.
  • a formulation featured herein can be delivered intravitreally, e.g., to treat disorders that are associated with, for example, the posterior segment of the eye.
  • Methods of intravitreal administration are known in the art and include, for example, intraocular injection, implantable devices.
  • the formulation is administered intravitreally using an implantable device.
  • the formulation comprises a thermal stabilizer, e.g., sorbitol.
  • the sorbitol is present at a concentration of ⁇ 5% w/v.
  • Implantable devices can be, for example, nonbiodegradable devices such as polyvinyl alcohol-ethylene vinyl acetate polymers and polysulfone capillary fibers, biodegradable devices such as polylactic acid, polyglycolic acid, and polylactic-co-glycolic acid, polycaprolactones, and polyanhydrides. Devices can be delivered in forms such as nanoparticles, liposomes, or microspheres.
  • nonbiodegradable devices such as polyvinyl alcohol-ethylene vinyl acetate polymers and polysulfone capillary fibers
  • biodegradable devices such as polylactic acid, polyglycolic acid, and polylactic-co-glycolic acid, polycaprolactones, and polyanhydrides.
  • Devices can be delivered in forms such as nanoparticles, liposomes, or microspheres.
  • a formulation featured in the invention can be administered as a fixed dose, as weight determined dose (e.g., mg/kg), or as an age determined dose.
  • the formulations described herein can be administered, for example, four times a day; three times a day; twice a day; once every day; every other day; every third, fourth or fifth day; every week; every two weeks; every three weeks; every four weeks; every five weeks; monthly; every two months; every three months; every four months; every six months; or as needed (ad libitum).
  • a pharmaceutical composition can include a “therapeutically effective amount” of an agent described herein.
  • a therapeutically effective amount of an agent can vary according to factors such as the disease state, age, sex, and weight of the individual, and the ability of the compound to elicit a desired response in the individual, e.g., amelioration of at least one disorder parameter (e.g., sign), or amelioration of at least one symptom of the disorder (and optionally the effect of any additional agents being administered).
  • a therapeutically effective amount is also one in which any toxic or detrimental effects of the composition are outweighed by the therapeutically beneficial effects.
  • a “therapeutically effective amount” is determined in a population of individuals and the amount is effective in ameliorating at least one symptom or indication of a cytokine-related disorder, e.g., an IL-6-related disorder in at least 5%, 10%, 25%, 50%, 70%, 75%, 80%, 85%, 90%, 95%, or 100% of an affected population.
  • a formulation is typically administered in a therapeutically effective amount.
  • a therapeutically effective formulation is a vehicle formulation.
  • a therapeutically effective formulation comprises a therapeutic protein.
  • compositions can be administered using medical devices as described herein and as known in the art, e.g., implants, infusion pumps, hypodermic needles, and needleless hypodermic injection devices.
  • a device can include, e.g., one or more housings for storing pharmaceutical compositions, and can be configured to deliver unit doses of the IL-6a, e.g., IL-6 antibody or fragment thereof described herein, and optionally a second therapeutic agent.
  • the doses can be fixed doses, i.e., physically discrete units suited as unitary dosages for the subjects to be treated; each unit can contain a predetermined quantity of an IL-6a, e.g., an IL-6 antibody or fragment thereof described herein, calculated to produce the desired therapeutic effect in association with a pharmaceutical carrier and optionally in association with another agent, e.g., such as those available as over the counter or prescribed products.
  • an IL-6a e.g., an IL-6 antibody or fragment thereof described herein
  • the formulation is administered to a subject having the disorder in an amount and for a time sufficient to induce a sustained improvement in at least one sign or symptom of the disorder.
  • An improvement is considered “sustained” if the subject exhibits the improvement over a prolonged period, e.g., on at least two occasions separated by one to four weeks.
  • the degree of improvement can be determined based on signs or symptoms, and can also employ questionnaires that are administered to the subject, such as quality-of-life questionnaires.
  • Improvement can be induced by repeatedly administering a dose of the formulation until the subject manifests an improvement over baseline for selected signs and/or symptoms.
  • the amount of improvement can be evaluated by repeated administration over a period of at least a month or more, e.g., for one, two, or three months or longer, or indefinitely.
  • the agent can be administered for a period of one to six weeks or even as a single dose.
  • treatment can be continued indefinitely at the same level or at a reduced dose or frequency. Treatment can also be discontinued, e.g., upon improvement or disappearance of signs or symptoms. Once treatment has been reduced or discontinued, it may be resumed if symptoms should reappear.
  • Diseases that can be treated with an IL-6a of the invention include those diseases in which IL-6 expression, e.g., elevated IL-6 expression, is associated with the disease state or as a prerequisite to the disease state.
  • diseases include those in which angiogenesis and inflammation driven by IL-6 contribute to disease pathology.
  • This includes diseases in which IL-6 is elevated compared to normal levels, e.g., diseases in which IL-6 is elevated in the vitreous (such as, e.g., diabetic macular edema, diabetic retinopathy, and uveitis) or tissues of the eye.
  • IL-6 related diseases include certain eye diseases including, without limitation, dry eye (e.g., dry eye disease or dry eye syndrome), allergic conjunctivitis, uveitis, age-related macular degeneration (AMD) (wet (exudative) AMD or dry (atrophic) AMD), proliferative diabetic retinopathy (PDR), diabetic macular edema (DME), Rhegmatogenous retinal detachment (RRD), retinal vein occlusion (RVO), neuromyelitis optica (NMO), or myopic choroidal neovascularization.
  • eye diseases including, without limitation, dry eye (e.g., dry eye disease or dry eye syndrome), allergic conjunctivitis, uveitis, age-related macular degeneration (AMD) (wet (exudative) AMD or dry (atrophic) AMD), proliferative diabetic retinopathy (PDR), diabetic macular edema (DME), Rhegmatogenous retinal
  • ocular disorders that can be treated include ocular cancers, e.g., cancers that affect the eye and the vicinity of the eye, e.g., the eye socket or the eyelids. Accordingly, the invention includes treating a subject having an IL-6 related disease with an IL-6a described herein.
  • ocular cancers e.g., cancers that affect the eye and the vicinity of the eye, e.g., the eye socket or the eyelids.
  • the invention includes treating a subject having an IL-6 related disease with an IL-6a described herein.
  • the term “treat” refers to the administration of an agent described herein to a subject, e.g., a patient, in an amount, manner, and/or mode effective to improve a condition, symptom, or parameter associated with a disorder, e.g., a disorder described herein, or to prevent the onset or progression of a disorder, to either a statistically significant degree or to a degree detectable to one skilled in the art.
  • the treatment can be to cure, heal, alleviate, relieve, alter, remedy, ameliorate, palliate, improve or affect the disorder, the symptoms of the disorder or the predisposition toward the disorder.
  • An effective amount, manner, or mode can vary depending on the subject and may be tailored to the subject. Exemplary subjects include humans, primates, and other non-human mammals.
  • a formulation featured in the invention can also be given prophylactically to reduce the risk of the occurrence of a disorder or symptom or sign thereof.
  • the IL-6 related disease is an inflammatory disease. In some embodiments, the disease is glaucoma.
  • the disease is ocular pain, e.g., pain associated with an ocular disease or disorder.
  • treatment of a subject also includes determining whether the subject has an IL-6 associated disease, and optionally, whether the subject is resistant to other non-IL-6 inhibitory treatments such as steroids or anti-VEGF agents.
  • the formulations described herein can be administered to a subject having or at risk for such IL-6 related diseases.
  • the IL-6 related disease or disorder can be an inflammatory disorder such as described below.
  • the formulations described herein can be administered to a subject having or at risk for such IL-6 mediated inflammatory disorders.
  • the formulations featured in the invention are particularly suited for use in ocular disorders, e.g. ocular disorders in which it is desired to administer the IL-6 antagonist, e.g., IL-6 antibody or fragment thereof described herein, directly to the eye, or locally to the area of the eye.
  • ocular disorders e.g. ocular disorders in which it is desired to administer the IL-6 antagonist, e.g., IL-6 antibody or fragment thereof described herein, directly to the eye, or locally to the area of the eye.
  • Subjects having a dry eye disorder can exhibit inflammation of the eye, and can experience scratchy, stingy, itchy, burning or pressured sensations, irritation, pain, and redness. Dry eye disorders can be associated with excessive eye watering and insufficient tear production.
  • a formulation featured in the invention can be administered to such a subject to ameliorate or prevent the onset or worsening of one or more such symptoms.
  • a formulation featured in the invention can also be used to mitigate pain, e.g., ocular pain, such as pain due to neuroinflammation, in a subject.
  • the embodiments described herein include methods of treating animals having IL-6-related disorders, for example, dry eye disorders.
  • Dry eye can be a serious disorder in, for example canines.
  • disorders in dogs associated with dry eye include congenital disorders, infections (e.g., canine distemper virus), drug induction (e.g., by sulfa antibiotics), and removal of the tear gland of the third eyelid (“cherry eye”).
  • Dry eye disorders are also commonly seen in certain dog breeds, for example, Cocker Dog, Shih Tzu, Lhasa Apso, Bulldog, Schnauzer, and West Highland White Terrier.
  • Other non-limiting examples of animals that can be treated include cats and horses.
  • the formulations of the present invention can be administered to a subject having an allergic reaction affecting the eye, e.g., a subject experiencing severe allergic (atopic) eye disease such as, e.g., allergic conjunctivitis.
  • the formulation can be administered topically. See also, e.g., Keane-Myers et al. (1999) Invest Ophthalmol Vis Sci, 40(12): 3041-6.
  • the formulations featured in the invention can be administered to a subject who has or is at risk for diabetic retinopathy. See, e.g., Demircan et al. (2006) Eye 20:1366-1369 and Doganay et al. (2006) Eye, 16:163-170
  • Uveitis includes acute and chronic forms and includes inflammation of one or more of the iris, the ciliary body, and the choroid.
  • Chronic forms may be associated with systemic autoimmune disease, e.g., Behçet's syndrome, ankylosing spondylitis, juvenile rheumatoid arthritis, Reiter's syndrome, and inflammatory bowel disease.
  • systemic autoimmune disease e.g., Behçet's syndrome, ankylosing spondylitis, juvenile rheumatoid arthritis, Reiter's syndrome, and inflammatory bowel disease.
  • inflammation is primarily in the iris (also ulceris).
  • Anterior uveitis can affect subjects who have systemic autoimmune disease, but also subjects who do not have systemic autoimmune disease.
  • Intermediate uveitis involves inflammation of the anterior vitreous, peripheral retina, and ciliary body, often with little anterior or chorioretinal inflammation.
  • Pan planitis results from inflammation of the pars plana between the iris and the choroid.
  • Posterior uveitis involves the uveal tract and primarily the choroid, and is also referred to as choroiditis.
  • Posterior uveitis can be associated with a systemic infection or an autoimmune disease. It can persist for months and even years.
  • the formulations featured in the invention can be administered to a subject to treat any of the foregoing forms of uveitis. See also e.g., Tsai et al. (2009) Mol Vis 15:1542-1552 and Trittibach et al. (2008) Gene Ther. 15(22): 1478-88.
  • the formulations featured in the invention are used to treat a subject having or at risk for age-related macular degeneration (AMD), e.g., wet (exudative) AMD or dry (atrophic) AMD.
  • AMD age-related macular degeneration
  • the formulations can be applied topically to the eye, injected (e.g., intravitreally) or provided systemically. See, e.g., Olson et al. (2009) Ocul Immunol Inflamm 17(3):195-200.
  • DME Diabetic Macular Edema
  • Diabetic macular edema involves occlusion and leakage of retinal blood vessels, causing reduced visual acuity and potentially blindness.
  • Standard treatments for DME include local administration of steroids or anti-VEGF antibodies.
  • DME diabetic macular edema
  • the pathogenesis of diabetic macular edema involves components of angiogenesis, inflammation, and oxidative stress.
  • IL-6 is induced by hypoxia and hyperglycemia and can increase vascular inflammation, vascular permeability, and pathologic angiogenesis.
  • IL-6 can directly induce VEGF expression and can promote choroidal neovascularization in animal models.
  • ocular IL-6 levels are positively correlated with macular thickness and disease severity.
  • IL-6 levels are reportedly elevated in patients who fail anti-VEGF therapy while decreasing in anti-VEGF responsive patients. Accordingly, administration of an IL-6a as described herein is useful for treatment of diabetics in combination with an anti-VEGF therapeutic or as an alternative to anti-VEGF treatment, including for patients who do not respond to anti-VEGF therapy. Treatment of macular edema with an IL-6a may also improve safety by removing the need to completely inhibit either mechanism to inhibit the pathology, thus preserving some of the desired, physiological roles of each cytokine. Accordingly, local IL-6a treatment in combination with VEGF inhibition can decrease the dose frequency and reduce adverse effects of treatment.
  • an IL-6a as described herein can be used to treat DME subjects who are refractive to steroid therapy, anti-VEGF therapy, or both.
  • an IL-6a e.g., an IL-6 antibody or fragment thereof as described herein, is used in combination with anti-VEGF therapy or steroid therapy, e.g., to treat DME.
  • the formulations provided herein comprise an anti-VEGF agent or a steroid.
  • a formulation described herein can be administered by any mode to treat an ocular disease.
  • the agent can be delivered by a parenteral mode.
  • the formulation can be delivered directly to the eye or in the vicinity of the eye.
  • the formulation can be administered topically, intraocularly, intravitreally, e.g., by intravitreal injection, or subconjuntivally.
  • the formulations described herein can also be used to treat disorders such as cancer, e.g., an ocular cancer (a cancer in the eye or in the vicinity of the eye), prostate cancer, leukemia, multiple myeloma, inflammatory (such as chronic inflammatory proliferative diseases) and autoimmune disease, e.g., rheumatoid arthritis, Castleman's disease (giant or angiofollicular lymph node hyperplasia, lymphoid hamartoma, angiofollicular lymph node hyperplasia), juvenile idiopathic arthritis (including polyarticular juvenile idiopathic arthritis and systemic juvenile idiopathic arthritis), Still's disease (encompassing juvenile idiopathic arthritis and adult onset Still's disease), adult onset Still's disease, amyloid A amyloidosis, polymyalgia rheumatica, remitting seronegative symmetrical syn
  • cancer e.g., an ocular cancer (a cancer in the eye or
  • IL-6 antagonists are also useful for treatment of certain neurologic diseases. Accordingly, in some cases, the formulations described herein can be used for treating depression and Alzheimer's disease.
  • TRAPS TNF-receptor-associated periodic syndrome
  • Example 1 Formulation Study by kD
  • An initial screen was performed to identify promising excipients and formulations for an IL-6 antibody, EBI-031, by assessing the kD of the antibody in each formulation.
  • Diffusion interaction parameter, kD was determined using a Wyatt DyanPro plate reader, which measures the diffusion coefficient of nano-particles in free solution.
  • Concentrated EBI-031 was serially diluted in formulation buffer to achieve the desired concentration.
  • the diffusion coefficient for EBI-031 monomer was measured using multiple replicates. The average diffusion coefficient was plotted versus EBI-031 concentration.
  • Diffusion coefficient, kD was obtained by dividing the slope of the graph by the y-value.
  • the tested formulations contained 20 mM of a buffer selected from citrate, histidine, or phosphate, and one of the following: 5% sucrose, 5% trehalose, 5% sorbitol, or 150 mM NaCl.
  • the tested formulations containing citrate buffer had a pH of 6.0 or 6.5
  • the formulations containing histidine or phosphate buffer had a pH of 6.0, 6.5, or 7.0.
  • Table 2 shown below summarizes the results from the kD formulation screen.
  • the formulations tested contained EBI-031 at 1 mg/ml, 20 mM of a buffer, and a tested excipient (such as a tonicity agent or an amino acid).
  • the buffers used were selected from: Tris buffer at pH 7.5, phosphate buffer at pH 6.5 or pH 7.5, histidine buffer at pH 6.5, citrate buffer at pH 5.5, or acetate buffer at pH 5.5.
  • the tested excipients were selected from: 5% sorbitol, 10% sucrose, 5% trehalose, 5% xylitol, 150 mM sodium chloride, 0.2M arginine, or 0.2M glycine.
  • the particular formulations tested are shown in the Table 3 below. The samples were incubated at 38° C. and stability was assessed after 1 and 6 weeks.
  • DLS Dynamic light scattering
  • Example 3 Product Purity Formulation Study at 0 and 7 Days
  • the tested formulations contained 20 mM of a base buffer, where the buffer was acetate (pH 5.5), citrate (pH 6.0 or pH 6.5), histidine (pH 6.5), phosphate (pH 6.5, pH 7.0, or pH 7.5), and tricine (pH 8.5); sodium chloride at either 20 mM and 150 mM; and one of the following excipients: 10% sucrose, 5% sorbitol, 0.1% polysorbate-20, 0.1% polysorbate-80, 0.1% poloxamer P188, and 0.2M arginine.
  • Tested formulations were prepared in a 96 well microdialysis plate.
  • Microdialysis plate wells were filled with stock buffer (20 mM of buffer) and excipient solutions at 2 ⁇ final concentration (e.g., 900 ⁇ l 2 ⁇ Buffer+900 ⁇ L 2 ⁇ excipient solution).
  • the plate was mixed on a plate shaker to ensure mixing prior to sample addition. Protein samples were added to the appropriate wells.
  • the organization of the formulations on the plate is shown in Table 4. Samples were dialyzed on a plate shaker at room temperature for 2 hours. Sample well inserts were transferred to a second buffer/excipient plate for overnight dialysis in fresh solutions.
  • each sample was recovered and transferred into clear 300 ⁇ L glass vials. All samples were clear and colorless.
  • the volumes recovered from each dialysis cassette varied in certain buffer conditions (particularly in the 10% sucrose buffers).
  • each sample was then analyzed using a NANODROP® Spectrophotomer (Thermo Scientific); however the measurements were variable, possibly due to high viscosity/high protein concentration.
  • An aliquot of each sample was then diluted to 100-fold in size exclusion-UPLC (SE-UPLC) mobile phase buffer to approximately 0.994 mg/mL (based on starting concentration) and analyzed on the NANODROP® Spectrophotomer. Results of the recovery are shown in Table 4.
  • SE-UPLC size exclusion-UPLC
  • the main IgG peak purity was determined by SE-UPLC.
  • the main IgG peak purity with respect to pH appeared to reduce slightly as the pH of the base buffer increased ( FIG. 2A ).
  • the excipient arginine had a positive impact on product purity ( FIG. 2B ). This effect was observed at both high and low salt concentrations. The other excipients may have longer term impact on product stability.
  • the concentration of the samples at day 7 was analyzed at 100-fold dilution by SEC Mobile phase A (NANODROP® Spectrophotomer).
  • the expected protein concentration following 100-fold dilution 0.994 mg/mL
  • the results of the NANODROP® Spectrophotomer analysis are shown in Table 6.
  • SE-UPLC analysis was also performed at day 7. SE-UPLC chromatograms were overlayed according to buffer to assess the effect of the different salt concentrations and excipients on the purity of the samples. The results from SE-UPLC analysis are summarized in FIGS. 3A-3E . The data also indicates a trend towards decreased main peak percentage and increased HMW peaks with increasing pH. Purity is also reasonably consistent at low pH ( FIG. 4 ). Histidine buffer was shown as being an optimal buffer for all excipients. Sorbitol was also shown as being the second best excipient in formulations with histidine buffer at pH 6.5 ( FIG. 5 ).
  • FIG. 6 is a graph showing the main peak purity (%) with respect to the different salt concentrations and excipients for the samples containing histidine buffer. On the X-axis, the numbers 1-12 represent the salt concentration and excipients present in columns 1-12 listed in Table 6.
  • samples were prepared containing 50 and 5 mg/ml of EBI-031 in 20 mM histidine, 20 mM sodium chloride, 4% sorbital, at pH 6.5 with: A) no detergent, B) 0.1% Tween-20, C) 0.1% Tween-80, D) 0.03% Tween-20, or E) 0.03% Tween-80.
  • Visual inspection of vials at the start and end of the study was conducted. At the beginning of the study, all samples looked clear by visual inspection. Controls were non-agitated samples at 2-8° C. (200 ⁇ l of sample).
  • the samples (800 ⁇ l) were agitated by Thermomixer at 400 rpm at 2-8° C., then aliquots were diluted to 1 mg/ml for analysis using SE-UPLC.
  • the remaining samples (500 ⁇ l), following removal of material for SE-UPLC and osmolality, were reagitated by Thermomixer at 1000 rpm at 2-8° C. for 4 hours, then aliquots were diluted to 1 mg/ml for analysis by SE-UPLC.
  • the remaining samples were reagitated by Thermomixer at 1000 rpm at 25° C. for 3 hours, then aliquots diluted to 1 mg/ml for analysis by SE-UPLC.
  • the remaining samples were reagitated by Thermomixer at 1000 rpm at 25° C. overnight.
  • FIGS. 8A-8C Results from the SE-UPLC analysis in the second agitation study are summarized in FIGS. 8A-8C .
  • the results are represented in graph format by analysis of Total Peak Area ( FIG. 9A ), IgG Main Peak Purity ( FIG. 9B ), High Molecular Weight Species ( FIG. 9C ), and Low Molecular Weight Species ( FIG. 9D ).
  • Concentration analysis was also performed on the samples by comparing the results from Solo VPE (Table 7B) and NANODROP® Spectrophotomer analysis (Table 7C), with the content estimated from SE-UPLC total peak area.
  • the Final Formulation Buffer R (20 mM Histidine, 20 mM Sodium Chloride, 4% Sorbitol, 0.03% Tween-20) will be prepared to flush the final 0.2 ⁇ m filter. To do this, 9 L of Buffer R will be made and 1 L of Buffer Q will be added (See Table 8 for details). The materials to be used in the GMP manufacture are detailed in Table 9.
  • the starting material was at 70.3 g/L. This material was diluted to a target of 55.5 g/L with Buffer R (20 mM Histidine, 20 mM Sodium Chloride, 4% Sorbitol). The product was then diluted 9 parts product to 1 part Buffer Q (20 mM Histidine, 20 mM Sodium Chloride, 4% Sorbitol, 0.3% Tween-20) to achieve a final EBI-031 concentration of 50.6 g/L and a final Tween-20 concentration of 0.03%.
  • Buffer R 20 mM Histidine, 20 mM Sodium Chloride, 4% Sorbitol
  • Buffer Q 20 mM Histidine, 20 mM Sodium Chloride, 4% Sorbitol, 0.3% Tween-20
  • a portion of the 50.6 g/L sample was diluted 1 part product to 1 part Buffer U (20 mM Histidine, 20 mM Sodium Chloride, 4% Sorbitol, 0.03% Tween-20) to achieve a final concentration of 25.3 g/L.
  • a portion of the 25.3 g/L material was further diluted 1 part product to 4 parts Buffer U to achieve a final concentration of 5.1 g/L. All concentrations were determined by A 280 with A 320 correction.
  • EBI-031 is an IgG2 antibody (sequences are provided in Table 1). As discussed previously, IgG2 antibodies exist in three different structural isoforms, IgG2-A, IgG2-B, and IgG2-AB isoforms ( FIG. 10 ). In this example, experiments were performed to identify the structural isoforms in EBI-031 samples and distribution thereof from different sample sources and in the presence of a reducing agent.
  • Reversed-phase high-performance liquid chromatograph (RP-HPLC) was used to resolve the various structural isoforms of EBI-031.
  • An enhanced analytical RP-HPLC method that has been used previously for resolving IgG2 disulfide-mediated structural isoforms (see, Dillon et al., Journal of Chromatography A, 2006, 1120:112-120) was optimized for resolving EBI-031.
  • EBI-031 samples containing approximately 30 ⁇ g was loaded onto a Zorbax 300SB-C8 column (150 mm ⁇ 2.1 mm, 5.0 ⁇ m, 300 ⁇ ).
  • the column temperature was set at 75° C.
  • Mobile phase A was water containing 0.1% TFA
  • mobile phase B was 55% IPA, 40% ACN, 4.9% water and 0.1% TFA.
  • the flow rate was 0.5 mL/min.
  • the column was initially equilibrated with 90% mobile phase A and 10% mobile phase B for 2 min followed by a 2 min step gradient from 10 to 25% B. Elution was achieved with a linear gradient of 25-32% B over 21 min.
  • UV absorbance was monitored at 214 nm and/or 280 nm.
  • the samples were treated with 5 mM DTT and 10 mM cysteine at room temperature for 2 min and then analyzed on the RP-HPLC method ( FIG. 11 ).
  • DTT which is a potent reducing agent
  • cysteine which is a milder reducing agent compared to DTT
  • the data demonstrates that the RP-HPLC method resolved the structural isoforms with different disulfide connectivity.
  • the different disulfide bonding structures were confirmed by non-reduced peptide mapping and mass spectrometry analysis: the early eluting peak (Peak 1) contains the IgG2-AB isoform and the late eluting peak (Peak 2) contains the IgG2-A isoform.
  • the early eluting peak contains the IgG2-AB isoform
  • Peak 2 contains the IgG2-A isoform.
  • IgG2-B isoform B Peak 0
  • EBI-031 samples collected from different EBI-031-expressing cell lines were analyzed to compare the isoform distribution of the antibodies produced.
  • EBI-031 samples were collected from a 200 L scale culture of a clonal cell line, a 10 L scale culture from a parental cell line, and a stably transfected pool of cells.
  • EBI-031 was purified using a three-step chromatography method from the clonal and parental EBI-031 expressing cell lines.
  • EBI-031 was purified from the stably transfected pool of cells using Protein A purification. The samples were analyzed by the methods described above.
  • FIG. 12 shows that all three EBI-031 samples contained isoforms IgG2-A and IgG2-AB, but no substantial amount of IgG2-B.
  • This data demonstrates that the EBI-031 IgG2 antibody is produced in a less heterogeneous mixture than other IgG2 antibodies, whether the production is from a clonal EBI-031-expressing cell line, a parental EBI-031-expressing cell line, or from a heterogeneous cell population that stably expresses EBI-031.
  • FIG. 13 shows the distribution of the isoforms from the EBI-031 sample from the 200 L scale culture of a clonal EBI-031-expressing cell line, e.g., the top panel of FIG. 12 . The areas under the curves were also measured, and the distributions among the isoforms are shown in the table below the figure.

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Cited By (2)

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Publication number Priority date Publication date Assignee Title
US20220033492A1 (en) * 2020-07-31 2022-02-03 Alamab Therapeutics, Inc. Anti-connexin antibody formulations
CN116157115A (zh) * 2020-08-31 2023-05-23 赛特瑞恩股份有限公司 稳定的药物制剂

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2020005630A (es) * 2017-11-30 2020-08-20 Bio Thera Solutions Ltd Formulacion liquida de anticuerpos humanizados para el tratamiento de enfermedades relacionadas con il-6.
CN112739378A (zh) * 2018-09-14 2021-04-30 田边三菱制药株式会社 含有人抗il-33单克隆抗体的医药用组合物
JP7411652B2 (ja) * 2018-10-31 2024-01-11 リヒター ゲデオン ニュイルヴァーノシャン ミューコェデー レースヴェーニュタールシャシャーグ 水性医薬製剤
CN116261448A (zh) * 2020-08-19 2023-06-13 百奥泰生物制药股份有限公司 用于治疗il-6相关疾病的包含高浓度人源化抗体的液体制剂
US20230073821A1 (en) * 2021-08-27 2023-03-09 International Business Machines Corporation Antigen-binding proteins targeting coronavirus (cov) variants
CN116554298A (zh) * 2022-02-25 2023-08-08 南京工业大学 一种强化细菌卷曲菌毛提高人表皮生长因子分泌生产效率的方法
WO2024073718A1 (en) * 2022-09-30 2024-04-04 Sonnet BioTherapeutics, Inc. Low dose il-6 formulations and methods of use thereof

Family Cites Families (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4548990A (en) 1983-08-15 1985-10-22 Ciba-Geigy Corporation Crosslinked, porous polymers for controlled drug delivery
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
US5476996A (en) 1988-06-14 1995-12-19 Lidak Pharmaceuticals Human immune system in non-human animal
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US6150584A (en) 1990-01-12 2000-11-21 Abgenix, Inc. Human antibodies derived from immunized xenomice
DE69120146T2 (de) 1990-01-12 1996-12-12 Cell Genesys Inc Erzeugung xenogener antikörper
US6075181A (en) 1990-01-12 2000-06-13 Abgenix, Inc. Human antibodies derived from immunized xenomice
US6673986B1 (en) 1990-01-12 2004-01-06 Abgenix, Inc. Generation of xenogeneic antibodies
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
JPH06508511A (ja) 1990-07-10 1994-09-29 ケンブリッジ　アンティボディー　テクノロジー　リミティド 特異的な結合ペアーの構成員の製造方法
US6300129B1 (en) 1990-08-29 2001-10-09 Genpharm International Transgenic non-human animals for producing heterologous antibodies
US5814318A (en) 1990-08-29 1998-09-29 Genpharm International Inc. Transgenic non-human animals for producing heterologous antibodies
US5625126A (en) 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US5770429A (en) 1990-08-29 1998-06-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5877397A (en) 1990-08-29 1999-03-02 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5874299A (en) 1990-08-29 1999-02-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US6255458B1 (en) 1990-08-29 2001-07-03 Genpharm International High affinity human antibodies and human antibodies against digoxin
DK0814159T3 (da) 1990-08-29 2005-10-24 Genpharm Int Transgene, ikke-humane dyr, der er i stand til at danne heterologe antistoffer
US5633425A (en) 1990-08-29 1997-05-27 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5789650A (en) 1990-08-29 1998-08-04 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5661016A (en) 1990-08-29 1997-08-26 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
WO1992009690A2 (en) 1990-12-03 1992-06-11 Genentech, Inc. Enrichment method for variant proteins with altered binding properties
DK1279731T3 (da) 1991-03-01 2007-09-24 Dyax Corp Fremgangsmåde til udvikling af bindende miniproteiner
DK1471142T3 (da) 1991-04-10 2009-03-09 Scripps Research Inst Heterodimere receptor-biblioteker under anvendelse af fagemider
DE4122599C2 (de) 1991-07-08 1993-11-11 Deutsches Krebsforsch Phagemid zum Screenen von Antikörpern
DE69229477T2 (de) 1991-09-23 1999-12-09 Cambridge Antibody Technology Ltd., Melbourn Methoden zur Herstellung humanisierter Antikörper
ES2241710T3 (es) 1991-11-25 2005-11-01 Enzon, Inc. Procedimiento para producir proteinas multivalentes de union a antigeno.
CA2124967C (en) 1991-12-17 2008-04-08 Nils Lonberg Transgenic non-human animals capable of producing heterologous antibodies
EP0640094A1 (en) 1992-04-24 1995-03-01 The Board Of Regents, The University Of Texas System Recombinant production of immunoglobulin-like domains in prokaryotic cells
CA2150262C (en) 1992-12-04 2008-07-08 Kaspar-Philipp Holliger Multivalent and multispecific binding proteins, their manufacture and use
CA2161351C (en) 1993-04-26 2010-12-21 Nils Lonberg Transgenic non-human animals capable of producing heterologous antibodies
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
FR2734739B1 (fr) 1995-06-01 1997-07-11 Gec Alsthom Stein Ind Dispositif de surveillance d'un broyeur a boulets
EP0795743A3 (en) 1996-03-15 1998-02-25 Japan Tobacco Inc. Method and apparatus for infra-red moisture measurement
US5839430A (en) 1996-04-26 1998-11-24 Cama; Joseph Combination inhaler and peak flow rate meter
WO1998023289A1 (en) 1996-11-27 1998-06-04 The General Hospital Corporation MODULATION OF IgG BINDING TO FcRn
US6277375B1 (en) 1997-03-03 2001-08-21 Board Of Regents, The University Of Texas System Immunoglobulin-like domains with increased half-lives
DK0979281T3 (da) 1997-05-02 2005-11-21 Genentech Inc Fremgangsmåde til fremstilling af multispecifikke antistoffer med heteromultimere og fælles bestanddele
EP0985039B1 (en) 1997-06-12 2008-02-20 Novartis International Pharmaceutical Ltd. Artificial antibody polypeptides
US6358058B1 (en) 1998-01-30 2002-03-19 1263152 Ontario Inc. Aerosol dispensing inhaler training device
ATE439592T1 (de) 1998-12-10 2009-08-15 Bristol Myers Squibb Co Proteingerüste für antikörper-nachahmer und andere bindende proteine
US6984720B1 (en) 1999-08-24 2006-01-10 Medarex, Inc. Human CTLA-4 antibodies
EP1916303B1 (en) 2000-11-30 2013-02-27 Medarex, Inc. Nucleic acids encoding rearranged human immunoglobulin sequences from transgenic transchromosomal mice
US20030157561A1 (en) 2001-11-19 2003-08-21 Kolkman Joost A. Combinatorial libraries of monomer domains
PT1390535E (pt) 2001-04-26 2010-10-04 Amgen Mountain View Inc Bibliotecas combinatórias de domínios monoméricos
JP5290489B2 (ja) * 2001-11-08 2013-09-18 アッヴィ・バイオセラピューティクス・インコーポレイテッド Ｉｇｇ抗体の安定な液体医薬製剤
WO2003094888A1 (en) 2002-05-07 2003-11-20 Control Delivery Systems, Inc. Processes for forming a drug delivery device
EP1572103A4 (en) 2002-11-15 2008-02-13 Centocor Inc ANTI-ANGIOGENIC USES OF IL-6 ANTAGONISTS
US20040191243A1 (en) * 2002-12-13 2004-09-30 Bei Chen System and method for stabilizing antibodies with histidine
BRPI0417270A (pt) 2003-12-23 2007-03-27 Rinat Neuroscience Corp anticorpos agonistas antitrkc e métodos para utilização dos mesmos
EP2213683B1 (en) 2004-08-04 2013-06-05 Mentrik Biotech, LLC Variant Fc regions
AU2005282700A1 (en) 2004-09-02 2006-03-16 Genentech, Inc. Heteromultimeric molecules
US8802820B2 (en) 2004-11-12 2014-08-12 Xencor, Inc. Fc variants with altered binding to FcRn
ATE550353T1 (de) 2005-12-30 2012-04-15 Merck Patent Gmbh Die bindung von mit il-6ralpha komplexiertem il-6 an gp130 verhindernde anti-il-6-antikörper
US20110059101A9 (en) 2006-03-13 2011-03-10 Ablynx N.V. Amino Acid Sequences Directed Against Il-6 And Polypetides Comprising The Same For The Treatment Of Diseases And Disorders Associated With Il-6 Mediated Signalling
EP2021463B1 (en) 2006-05-19 2016-11-23 Alder Biopharmaceuticals, Inc. Culture method for obtaining a clonal population of antigen-specific b cells
RU2009127888A (ru) 2007-02-16 2011-03-27 Вайет (Us) Препараты белка, содержащие сорбит
LT2164514T (lt) 2007-05-21 2017-03-10 Alderbio Holdings Llc Il-6 antikūnai ir jų naudojimas
EP2198007B1 (en) * 2007-09-14 2017-10-25 Sanofi Pasteur Biologics, LLC Pharmaceutical compositions containing clostridium difficile toxoids a and b
CN101925611A (zh) 2007-11-21 2010-12-22 安姆根有限公司 Wise结合剂和表位
US20100187601A1 (en) 2007-12-12 2010-07-29 Fujio Masuoka Semiconductor device
NZ601913A (en) 2008-01-15 2014-02-28 Abbott Gmbh & Co Kg Powdered protein compositions and methods of making same
MX2011001371A (es) 2008-08-05 2011-06-16 Novartis Ag Composiciones y metodos para anticuerpos que se dirigen a la proteina de complementos c5.
CN102123796B (zh) 2008-09-12 2013-09-11 Kdf株式会社 喷水装置
US20120005773A1 (en) * 2008-10-01 2012-01-05 Aasen Eric D Transgenic plants with enhanced agronomic traits
US8992920B2 (en) * 2008-11-25 2015-03-31 Alderbio Holdings Llc Anti-IL-6 antibodies for the treatment of arthritis
KR20110091777A (ko) 2008-11-26 2011-08-12 글락소 그룹 리미티드 폴리펩티드, 항체 가변 도메인 및 길항제
WO2010088444A1 (en) * 2009-01-29 2010-08-05 Medimmune, Llc Human anti-il-6 antibodies with extended in vivo half-life and their use in treatment of oncology, autoimmune diseases and inflammatory diseases
JO3417B1 (ar) * 2010-01-08 2019-10-20 Regeneron Pharma الصيغ المستقرة التي تحتوي على الأجسام المضادة لمضاد مستقبل( interleukin-6 (il-6r
AU2011277999A1 (en) 2010-07-12 2013-01-10 Covx Technologies Ireland Limited Multifunctional Antibody Conjugates
EP2788097A4 (en) 2011-12-07 2015-09-09 Amgen Inc IGG2 DISULFIDE ISOFORMATION SEPARATION
EP2854844A4 (en) 2012-06-01 2016-11-23 Ophthotech Corp COMPOSITIONS COMPRISING ANTI-PDGF APTAMER AND VEGF ANTAGONIST
RS62509B1 (sr) 2012-07-13 2021-11-30 Roche Glycart Ag Bispecifična anti-vegf/anti-ang-2 antitela i njihova upotreba u lečenju očnih vaskularnih bolesti
EA201791717A1 (ru) * 2012-09-07 2018-03-30 Кохерус Байосайенсис, Инк. Стабильные водные составы адалимумаба
BR112015008186A2 (pt) * 2012-10-25 2017-09-19 Medimmune Llc formulação de um anticorpo estável e de baixa viscosidade
CN104903349B (zh) * 2012-11-08 2018-10-19 十一生物治疗股份有限公司 Il-6拮抗剂及其应用
SG11201510315TA (en) * 2013-07-12 2016-01-28 Ophthotech Corp Methods for treating or preventing ophthalmological conditions
EP2898896A1 (en) 2014-01-22 2015-07-29 Université Pierre et Marie Curie (Paris 6) Agents for use in the treatment of retinal inflammation
WO2016073894A1 (en) 2014-11-07 2016-05-12 Eleven Biotherapeutics, Inc. Therapeutic agents with increased ocular retention
KR102636726B1 (ko) * 2014-11-07 2024-02-13 에프. 호프만 라-로셰 엘티디 향상된 il-6 항체

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220033492A1 (en) * 2020-07-31 2022-02-03 Alamab Therapeutics, Inc. Anti-connexin antibody formulations
US11912764B2 (en) * 2020-07-31 2024-02-27 Alamab Therapeutics, Inc. Anti-connexin antibody formulations
CN116157115A (zh) * 2020-08-31 2023-05-23 赛特瑞恩股份有限公司 稳定的药物制剂

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