US20100239609A1 - Formulation of Sugar Solutions for Continuous Ultracentrifugation for Virus Purification - Google Patents

Formulation of Sugar Solutions for Continuous Ultracentrifugation for Virus Purification Download PDF

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US20100239609A1
US20100239609A1 US12/598,749 US59874908A US2010239609A1 US 20100239609 A1 US20100239609 A1 US 20100239609A1 US 59874908 A US59874908 A US 59874908A US 2010239609 A1 US2010239609 A1 US 2010239609A1
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virus
sucrose
gradient
sugar
buffered
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Manfred Reiter
Leopold Grillberger
Wolfgang Mundt
Artur Mitterer
Horst Schafhauser
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Baxter Healthcare SA
Baxter International Inc
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Baxter International Inc
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Definitions

  • the present invention relates to the field of virus purification.
  • Viruses are used for vaccination and in the field of gene therapy. It is possible for many viruses or virus-like particles to safely and efficiently propagate in host cells.
  • Several publications describe the purification of viruses from host cells, mostly concentrating on the use of specific chromatographic matrices for purification of the virus from a host cell lysate (see, e.g. U.S. Pat. No. 6,008,036).
  • Other methods as described, for example, in U.S. Pat. No. 6,048,537 employ continuous sucrose gradient centrifugation, which delivers products with less antigen purity and requires further purification steps by centrifugation. Such methods also suffer from antigen aggregation, which can lead to a loss of viral antigen, or inhibit viral inactivation steps.
  • Viral aggregation can also inhibit the yield of chromatographic processes, which are time and cost intensive and difficult to adapt to a large scale production. It is therefore a goal of the present invention to provide a method to purify viruses, in particular from host cell samples, that is simple but still is capable of providing whole virus antigen fractions in high purity with reduced viral antigen aggregation.
  • FIG. 1 Microphotograph of PMVHs purified with the conventional ( FIG. 1 a ) and the inventive ( FIG. 1 b ) ultracentrifugation procedures.
  • the present invention provides a method for purification of a virus or virus antigen comprising providing a virus preparation and centrifugation of said virus preparation in a gradient of a sugar established by a first layer A of the sugar in a concentration of a sucrose equivalent of 34% to 50% (w/w %) and a second layer B of the sugar in a concentration of a sucrose equivalent of 50% to 65% (w/w %) sucrose, in an aqueous buffer, with one buffer component being a buffer component having a pKa value between 6.0 and 9.4, and collecting said virus or virus antigen from the centrifugate, i.e. the product of the centrifugation process.
  • One aspect of the invention is to provide a method for purification of a virus or virus antigen.
  • the method involves providing a solution containing a sugar gradient established by centrifuging at least a first buffered sugar solution and at least a second buffered sugar solution.
  • the concentration of sugar in the first buffered sugar solution has a sucrose equivalent of 35% to 50% (w/w %), or in certain embodiments, 34% to 46% (w/w %).
  • the concentration of sugar second buffered sugar solution has a sucrose equivalent of 50% to 65% (w/w %), or in certain embodiments, 50% to 60% (w/w %).
  • the virus or the viral antigen is concentrated by centrifugation, allowing a separation from cell culture media components and/or host cells and host cell components.
  • the inventive method can minimize virus aggregation and virus loss at the centrifugation step of purification.
  • a first sugar solution in a physiological buffer is loaded generally vertically into a continuous ultracentrifugation apparatus to form a horizontal sugar solution layer A, and then a second, higher concentration sugar solution in the same or different physiological buffer is loaded into the apparatus to form a horizontal sugar solution layer B.
  • the apparatus is then activated, creating a sugar gradient that is most concentrated on outer wall of the apparatus, and that becomes less concentrated towards the center of the apparatus.
  • a virus containing harvest solution from a cell culture is then loaded into the apparatus, and the virus particles migrate to a location in the sugar gradient where their density is equivalent to the density of the gradient.
  • the sugar sucrose
  • a typical density range where this equilibration occurs is 36%-48% (w/w %) sucrose.
  • One aspect of this invention is the surprising discovery that by utilizing a two-layer solution approach, the volume of the peak pool can be increased as compared to conventional methods utilizing a single concentration of sucrose solution in water to form a sucrose gradient. This surprising effect is obtained even if sucrose is used and the maximum sucrose concentration in the gradient is the same as used in the conventional method.
  • the peak pool volume can be increased, for example, by two-fold. This increased peak pool volume reduces the aggregation of whole virus particles, or antigen by reduction of their respective concentrations.
  • Continuous ultracentrifugation processes are useful for purification of virus preparations.
  • Highly concentrated solutions of sugar e.g., sucrose or sorbitol
  • salt e.g. CsCl 2 or NaBr
  • sugar solutions without any additives have a low concentration of electrolytes and no buffer capacity.
  • one aspect of the invention is the realization that virus preparations in sugar solutions, such as sucrose solutions, have a strong tendency to form aggregates, dependent on the concentration of the virus particles, which can cause problems in the further processing for vaccine production.
  • inventive methods can effectively minimize these problems using sugar solutions (e.g., sucrose solutions at 55% w/w) with the addition of salt at physiological concentrations (e.g., NaCl at about 4-8 g/kg) and with the addition of a buffer (e.g., Tris, approximately 10-20 mmol/kg, followed by pH adjustment) to achieve physiological electrolyte and pH conditions in the gradient.
  • sugar solutions e.g., sucrose solutions at 55% w/w
  • salt at physiological concentrations e.g., NaCl at about 4-8 g/kg
  • a buffer e.g., Tris, approximately 10-20 mmol/kg, followed by pH adjustment
  • different sugar solutions are applied to build the gradient in the ultracentrifuge. As an example, 200 mL of a 55% w/w solution and 800 mL of a 42% w/w solution are loaded to the ultracentrifuge.
  • the smaller amount of higher concentration sucrose solution ensures that at the end of the ultracentrifugation the maximum sucrose concentration remains above 45% and the larger amount of 42% sucrose results in a more gradual gradient in the range of about 40% where the virus peak maximum typically occurs.
  • the volume of the peak pool can be increased, and therefore the virus particle suspension is diluted and results in less aggregation.
  • the diffusion of molecules or particles between the supernatant which is loaded to the ultracentrifuge and the gradient is modified by using such sucrose formulations.
  • Tris-buffer By way of example, a density gradient formed using approximately 42% and 55% (w/w %) sucrose solution in 20 mmol/kg Tris-buffer was found to be particularly useful for the influenza virus but can also be easily adapted for other viruses. Tris (trishydroxymethylaminomethane) is a useful buffer for pH ranges between 6.5 and 9.7. Although sucrose is used in this particular example of an embodiment of the invention, other sugars suitable for gradient centrifugation may be used. In addition, other buffer compounds, in particular organic buffer compounds, such as amines, can be used.
  • the buffer has a pKa value above 6, 6.2, 6.4, 6.6, 6.8, 7.2, 7.4, 7.6, 7.8 or 8.0 and below 9.4, 9.2, 9.0, 8.8, 8.6, 8.4 or 8.2.
  • Suitable buffers comprise for example HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), ACES (N-(2-Acetamido)-2-aminoethanesulfonic acid), bis-tris-methane or -propane, CAPS (N-cyclohexyl-3-aminopropanesulfonic acid) or CAPSO, PIPES (piperazine-N,N′-bis(2-ethanesulfonic acid)), phosphate buffer, or any other buffer used in the field of biochemistry.
  • the buffer is preferably chemically inert to biomolecules.
  • the centrifugation process can generate a peak pool fraction in which the virus particle is dispersed under physiological conditions with regard to electrolyte and pH conditions.
  • concentration of whole virus antigen in the peak pool fraction can be modified by adding different amounts of high and low concentration sugar solutions to define the gradient and therefore the volume of the peak fraction.
  • the volume of the peak fraction is also increased by the addition of salt and a suitable buffer (e.g. Tris-buffered saline (TBS)) alone, leading to a significantly higher yield of antigen and protein.
  • TBS Tris-buffered saline
  • This effect might be due to an unexpected improved mobility of proteins and virus antigen into a sugar gradient containing physiological concentrations of salt and a physiological pH.
  • Different ultracentrifuge conditions e.g., with and without preclarifier and different gravitational forces
  • the addition of electrolytes is greater than 50 mOsm/kg, 100 mOsm/kg, 150 mOsm/kg, 200 mOsm/kg or 250 mOsm/kg and less than 500 mOsm/kg, 450 mOsm/kg, 400 mOsm/kg or 350 mOsm/kg, preferably about 300 mOsm/kg.
  • the amount of electrolyte is calculated as equivalent to the osmolality of aqueous solutions without the van't Hoffs factor correction.
  • the contribution of the gradient-forming substance e.g., sucrose
  • the pH value can be in physiological range, preferably between 5.5 and 9.5, more preferably between 5.5 and 8.5, especially preferred above 5.5, 6, 6.5 or 7 and below 9.5, 9.0, 8.5, 8 or 7.5, in particular between 7.0 and 7.5 or between 7.0 and 7.4.
  • the sugar used to create a density gradient is sucrose.
  • this invention also contemplates the use of other sugars, including alcohol sugars, a non-limiting example of which is sorbitol.
  • the invention contemplates the use of hydrogenated sugars, linear sugars, modified sugars or any other sugar, provided that the sugar has a solubility in water sufficient to produce the solutions with the densities specified herein.
  • Combinations of sugars i.e., two or more sugars, either in the same or different layers, can also be used to generate the gradients described herein, provided that the corresponding sugar solutions that constitute those layers have the densities specified herein.
  • the volume of the gradient forming preparation is between 5% to 100%, preferably above 5%, 10% 15%, 20%, 25%, 30%, 32%, 35%, 40%, 45% or 50% or less than 100%, 90%, 80%; 75%, 70%, 65%, 60%, 55%, 50%, 48%, 45%, 40%, 35%, 30% or 25%, of the effective volume of the ultracentrifugation rotor or device applying centrifugal forces to the sample.
  • the volume of gradient forming preparation is between 1% to 75% of the effective volume of the ultracentrifugation rotor or device applying centrifugal forces to the sample.
  • the loading of the rotor containing the gradient forming material is carried out in a continuously or discontinuously repeated mode (i.e., either continuous ultracentrifugation or batch ultracentrifugation), where the loaded volume is preferably higher than the void volume of the rotor (total volume minus gradient volume), preferably more than 1 ⁇ , 2 ⁇ , 3 ⁇ , 5 ⁇ , 10 ⁇ , 20 ⁇ , 30 ⁇ , 40 ⁇ , 50 ⁇ , 60 ⁇ , 80 ⁇ or less than 300 ⁇ , 250 ⁇ , 200 ⁇ , 150 ⁇ , 120 ⁇ , 100 ⁇ , 80 ⁇ .
  • continuous ultracentrifugation is used.
  • the loaded volume is between 20 L to 50 L per 600 mL to 1000 mL void volume (1600 mL rotor volume minus 600 mL to 1000 mL void volume), which is about a 20 ⁇ to 80 ⁇ ratio. In another embodiment, the loaded volume is between 40 L to 100 L per 1200 mL to 2000 mL void volume (3200 mL rotor volume minus 1200 mL to 2000 mL void volume, which is about a 20 ⁇ to 80 ⁇ ratio).
  • a continuous gradient of the sugar is established.
  • a fraction of the centrifugate is collected above a sucrose concentration of 24% (equivalent to about 1.10 kg/l), 26%, 28%, 30%, 32%, 34%, 36%, 38%, 40%, 42%, 44% or 46% (w/w %) (equivalent to about 1.22 kg/l) and less than 66% (equivalent to about 1.32 kg/l), 63%, 60%, 58%, 56%, 54%, 52%, 50%, 48%, 46% 44% or 42% (w/w %) (equivalent to about 1.19 kg/l).
  • the viral product accumulates in this range.
  • a fraction of the centrifugate is collected between a sucrose concentration of about 30% and 54% (w/w %), more preferred between 36% and 48% (w/w %) sucrose.
  • Fractions collected from gradients formed from other sugars might have different concentrations (w/w %), but equivalent densities.
  • the densities of the collected fraction may be above 1.10 kg/l, 1.12 kg/l, 1.14 kg/l, 1.16 kg/l or 1.18 kg/l, 1.20 kg/l, 1.22 kg/l and below 1.32 kg/l, 1.30 kg/l, 1.28 kg/l, 1.26 kg/l, 1.24 kg/l, 1.22 kg/l or 1.20 kg/l.
  • the densities may be between 1.13 and 1.25 kg/l, more preferred between 1.16 and 1.22 kg/l.
  • the concentration of buffer or buffer component is in between 2 mmol/kg to 50 mmol/kg, preferably 5 mmol/kg to 40 mmol/kg, 10 mmol/kg to 40 mmol/kg, more preferably between 10 mmol/kg to 30 mmol/kg, more preferably between 18 mmol/kg to 25 mmol/kg, and is most preferred to be 20 mmol/kg.
  • the buffer is preferably aqueous with less than 5% water-miscible organic solvents, more preferably less than 2% organic solvents, and is most preferably free of organic solvents.
  • the buffer has a concentration of more than 2 mmol/kg, 5 mmol/kg, 10 mmol/kg, 12 mmol/kg, 14 mmol/kg, 15 mmol/kg, 17 mmol/kg, 18 mmol/kg, 19 mmol/kg or 20 mmol/kg or less than 50 mmol/kg, 40 mmol/kg, 35 mmol/kg, 30 mmol/kg, 25 mmol/kg, 23 mmol/kg or 21 mmol/kg.
  • the centrifugation can be performed preferably with a centrifugal force of at least 20,000 g, more preferably 30,000 g, more preferably 50,000 g, more preferably 70,000 g, most preferably 90,000 g, but in other embodiments, the centrifugal force is less than 200,000 g, less than 150,000 g, less than 120,000 g, less than 100,000 g, or less than 90,000 g.
  • the volume ratio of the initial layer A to the initial layer B (i.e. before application of the virus preparation) is between 20:1 to 1:1, preferably between 10:1 to 1.5:1, more preferably between 8:1 to 2:1 even more preferably between 6:1 to 3:1, and most preferably 4:1.
  • the ratio can be less than 20:1, 15:1, 10:1, 8:1, 5:1, 4:1, 3:1 or greater than 1:2, 1:1, 1.5:1, 2:1, 3:1 or 4:1.
  • the sugar-containing centrifugation fluid is two-layered (i.e., comprising the sugar layers A and B of different concentrations prior to addition of the virus preparation, below the filler layer of liquid in the ultracentrifuge).
  • the centrifugation fluid is comprised of 3, 4, 5, 6, 7 or even 8 different layers.
  • Each layer may have the same buffer as the other layers, or the buffers of each layer may be independently chosen.
  • the method of preparation does not comprise a preclarification step.
  • preclarification may be carried out in a preclarification chamber of the ultracentrifugation apparatus.
  • layer A comprises between 40% to 44% (w/w %) sucrose, preferably from 41% to 43% (w/w %) sucrose.
  • the sucrose concentration may be above 35% (equivalent to about 1.15 kg/l), 36%, 37%, 38%, 39%, 40%, 41% or 42% (w/w %) (equivalent to about 1.19 kg/l) or below 50% (equivalent to about 1.23 kg/l), 49%, 48%, 47%, 46%, 45%, 44%, 43% or 42% (w/w %) (equivalent to about 1.19 kg/l).
  • Layer A formed from another sugar might have a different concentration on a weight/weight basis, but will have a density that falls within the density ranges specified herein.
  • layer B comprises a sucrose solution having a concentration between 50% to 65% (w/w %), preferably between 52% to 58% (w/w %), and more preferably between 54% to 56% (w/w %).
  • the sucrose concentration (w/w %) may be above 50% (equivalent to about 1.23 kg/l), 51%, 52%, 53%, 54% or 55% (equivalent to about 1.26 kg/l) or below 66% (equivalent to about 1.32 kg/l), 64%, 62%, 61%, 60%, 59%, 58%, 57%, 56% or 55% (equivalent to about 1.26 kg/l).
  • Layer B formed from other sugars might have a different concentration on a weight/weight basis, but will have a density that falls within the density ranges specified herein.
  • the virus is an orthomyxovirus, in particular an influenza virus, preferably selected from influenza A and B.
  • viruses contemplated by the invention include viruses selected from the group of RNA virus families such as Reoviridae, Picornaviridae, Caliciviridae, Togaviridae, Arenaviridae, Retroviridae, Flaviviridae, Orthomyxoviridae, Paramyxoviridae, Bunyaviridae, Rhabdoviridae, Filoviridae, Coronaviridae, Astroviridae, Bornaviridae, and DNA virus families such as Adenoviridae, Papovaviridae, Parvoviridae, Herpesviridae, Poxviridae, Hepadnaviridae.
  • the virus is selected from the group consisting of Influenza A/Panama/2007/99, A/New Caledonia/20/99, and B/S
  • the virus harvest is prepared from cells inoculated with the virus.
  • the virus can be produced in any cells suitable for the production of viruses.
  • the cells are of an animal cell culture or cell line. Such cells may be from a specific tissue or embryonic cells such as embryonic eggs.
  • the animal is preferably a mammal or a bird.
  • the cells are avian, canine, rodent, or primate cells.
  • the cells are epithelial cells, in particular preferred kidney epithelial cells, such as Vero cells of an African green monkey.
  • the virus harvest applied to the ultracentrifugation apparatus may be the whole cell culture, or cell culture supernatant obtained after separating cells and/or portions thereof from the cell culture.
  • cells in the cell culture are allowed to settle in the culture vessel prior to drawing off a cell culture supernatant.
  • the cells may be lysed by chemical or mechanical methods, non-limiting examples of which include hypo or hypertonic lysis, detergent treatment, sonication, and mechanical disruption.
  • the virus is inactivated or fragmented (e.g., as described in WO 05/11800) prior to or after the centrifugation.
  • a vaccine of the virus may be prepared by methods known in the art.
  • a vaccine is an immunogenic composition of an antigenic substance, i.e. the (non-infections) virus, its hull, particles or its antigens, which an be used to immunize an animal, e.g. a mammal such as a human, or a bird.
  • the monovalent harvest is concentrated by ultracentrifugation.
  • a continuous flow centrifugation procedure can be applied for the manufacture of the Vero cell culture grown viral vaccine based on a sucrose gradient formed using a 50% (w/w %) sucrose solution in water.
  • the centrifuge rotor model used was equipped with a preclarifier.
  • a number of fermentation optimization approaches like change of the Vero cell culture medium by supplementing soy hydrolysate or elevated temperature conditions in the early phase of Influenza virus replication resulted in a more robust process as well as increased antigen yields. Therefore it turned out that the 50% (w/w %) sucrose/water gradient did not allow to achieve the desired recovery due to limitations in the antigen binding capacity.
  • a continuous flow ultracentrifuge CC40S with a C40CTS rotor (rotor volume 1,600 mL) or an Alfa Wassermann ultracentrifuge RK6 with an equivalent rotor was used. Sucrose gradient solutions were loaded to the rotor and then accelerated to the rotational speed of 20,000 to 35,000 rpm. After continuous loading with the inactivated harvest, the rotor was flushed with buffer to remove residual protein, which had not entered the gradient. After flushing, the rotor was decelerated and the ultracentrifugation was stopped, allowing the gradient to shift from the radial to axial direction of the rotor. Elution and fractionation was carried according to sucrose concentrations. A Coriolis type density detection unit and a UV 254 nm detection unit were used to monitor the sucrose and protein concentration.
  • Samples of the purified inactivated harvests were measured for total protein concentration, HA-SRD and Vero Antigen by ELISA according to standardized procedures to quantify yield and purity from the ultracentrifugation experiments.
  • sucrose gradients in TBS have several advantages over the standard gradients in the sucrose/water system.
  • the purification experiments (run 2) with sucrose in TBS gave increased antigen yields compared to reference run 1.
  • For the TBS preparations a significant reduction in the aggregate fraction could be observed.
  • the TBS sucrose gradient has several advantages over the standard system (sucrose/water), a loss in antigen yield was observed at higher loading (50 liters compared to 25 liters) of the gradient (run 3).
  • the peak pool volume increased, which is indicative for more diffusion between the loaded harvest and the gradient containing a buffer substance and other electrolytes.
  • This example shows an exemplary embodiment in which a sucrose gradient was modified to increase the volume of the peak pool fraction without changing the limits for fractionation.
  • a sucrose gradient with a reduced concentration of 42% (w/w %) was used, which resulted in a less steep sucrose gradient eluted from the ultracentrifuge.
  • a more concentrated sucrose/TBS solution of 50% (w/w %) was loaded subsequent to the 42% (w/w %) solution, which, as a higher concentration solution, formed a “cushion” to prevent from sedimentation of antigen on the wall of the rotor.
  • Table 2 shows purification runs using such a two-step gradient with 900 mL of a less concentrated sucrose/TBS solution (42% w/w sucrose, 11.7 mmol/kg TRIS, 4.7 g/kg NaCl) and 100 mL of a more concentrated sucrose/TBS solution (50% w/w, 10 mmol/kg TRIS, 4 g/kg NaCl), compared to a purification run using a gradient formed only with a 50% sucrose/TBS solution, applied in different volumes (viz., 600, 800, and 1000 mL). The tests were performed with preclarifier at 20,000 rpm (RCF appr. 30,000 g).
  • sucrose/TBS sucrose/TBS
  • HA- Antigen yield Peak Pool Purification Conditions/ SRD/protein (mg antigen/liter Volume Run Setup ratio (mg/mg) harvest) (mL) 1 Sucrose/TBS 50% 600 mL 0.58 1.3 346 2 Sucrose/TBS 50% 800 mL 0.58 1.3 325 3 Sucrose/TBS 50% 1000 mL 0.45 1.1 338 4 Sucrose/TBS 42% 900 mL + 0.53 1.5 713 Sucrose/TBS 50% 100 mL
  • the ultracentrifugation rotors can be run with or without preclarifier, therefore a comparison of 20,000 rpm (appr. 30,000 g) with preclarifier and 35,000 rpm (appr. 90,000 g) in a two step sucrose/TBS gradient (100 mL of 50% w/w sucrose, final concentration 10 mmol/kg TRIS, 4 g/kg NaCl and 900 mL of 39% w/w sucrose, final concentration 12.2 mmol/kg TRIS, 4.9 g/kg NaCl) was carried out.
  • the preclarifier and the rotor wall were swabbed with buffer and analyzed for antigen losses.
  • the results of this experiment are shown in Table 3.
  • the antigen yield could be increased by over 60% from 1.7 mg HA-SRD/L harvest to 2.8 mg HA-SRD/L harvest.
  • the main part of the missing antigen could be swabbed from the preclarifier, whereas only about 5% of the antigen was lost on the rotor wall of the ultracentrifuge.
  • the SRD/Protein ratio is only slightly reduced by omitting the preclarification prior to loading the harvest onto the sucrose/TBS gradient.
  • Initial formulations were carried out by preparing a 50% w/w sucrose/TBS gradient by mixing of 50% (weight) sucrose to 50% (weight) TBS buffer (20 mmol/kg TRIS, 8 g/kg NaCl) resulting in final concentrations of 10 mmol/kg TRIS and 4 g/kg NaCl. Subsequently, less concentrated formulations 42%-39% sucrose/TBS solutions were prepared with higher amounts of TBS, resulting in lower concentrations of sucrose but correspondingly higher concentrations of TRIS and NaCl (see previous examples). More concentrated solutions like, for example, 55% (w/w/) sucrose in TBS had therefore lower concentrations of TRIS and NaCl.
  • the same modified version of the two-step sucrose/TBS gradient was applied as in the previous example 5.
  • concentration of the higher density solution was increased to 55% w/w and a higher volume of 200 mL was used to create a more robust high density sucrose cushion in the gradient, after loading with 800 mL of 42% sucrose/TBS.
  • Final TRIS concentration in the gradient was increased to 20 mmol/kg and the final NaCl concentration was increased to 8 g/kg in both sucrose/TBS solutions.
  • Ultracentrifugation conditions were 35,000 rpm (90,000 g) without preclarifier, and fractionation was carried out between 48% to 36% sucrose.
  • Example 8 Three comparative experiments were carried out with the 2002/2003 strains (viz., A/New Caledonia/20/99, A/Panama/2007/99 and B/Shangdong/7/97).
  • the peak pool fractionation limits for these runs were defined with 48% to 36% (w/w %) sucrose.
  • the results are shown in Example 8 to Example 10.
  • the virus antigen quality measured as HA/total protein ratio and Vero-protein/HA ratio shows that this increase in doses per liter did not compromise antigen quality.
  • a slight increase in the HA/total protein from 0.41 to 0.60 was achieved with the new sucrose gradient conditions.
  • For the host cell protein/HA ratio no significant difference could be detected.
  • Under the sucrose/TBS gradient conditions for PMVH production aggregation of strain A/New Caledonia/20/99 was slightly reduced. This effect could be confirmed by microscopic observation (see, e.g., FIG. 1A and FIG. 1B ). At 400 ⁇ magnification the stronger, formation of aggregates is clearly visible when applying the previous sucrose/water gradient version ( FIG. 1A ), whereas despite increased yields, the peak pool fraction derived from the optimized ultracentrifugation procedure appears to be significantly more homogenous ( FIG. 1B ).
  • the virus antigen' quality measured as HA/total protein ratio and Vero-protein/HA ratio is acceptable. But, under the TBS sucrose gradient conditions for PMVH production, aggregation of strain Panama could be significantly reduced using the sucrose/TBS gradient.
  • inventive sucrose gradient allows efficient loading of the monovalent harvests.
  • inventive methods described herein including 1) increased virus yield of at least 25%, 2) a strain dependant HA/total protein ratio of 0.34 to 0.77, 3) a strain dependant host cell protein content of 2% to 7% and 4) minimized virus aggregation in the sucrose peak pool (PMVH).

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