US20060030036A1 - Chips for multiplex analyses - Google Patents

Chips for multiplex analyses Download PDF

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Publication number
US20060030036A1
US20060030036A1 US11/136,841 US13684105A US2006030036A1 US 20060030036 A1 US20060030036 A1 US 20060030036A1 US 13684105 A US13684105 A US 13684105A US 2006030036 A1 US2006030036 A1 US 2006030036A1
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Prior art keywords
chip
well
reaction
micro
micro well
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US11/136,841
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Inventor
Victor Joseph
Amjad Huda
Alnoor Shivji
Jie Zhou
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Wafergen Inc
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Wafergen Inc
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Priority to US11/136,841 priority Critical patent/US20060030036A1/en
Assigned to WAFERGEN, INC. reassignment WAFERGEN, INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: HUDA, AMJAD, JOSEPH, VICTOR, SHIVJI, ALNOOR, ZHOU, JIE
Publication of US20060030036A1 publication Critical patent/US20060030036A1/en
Abandoned legal-status Critical Current

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    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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Definitions

  • the apparatus of the present invention is capable of performing a vast diversity of chemical reactions.
  • the subject apparatus is particularly suited for performing enzymatic reactions, including but not limited to nucleic acid amplification reaction that encompasses PCR, quantitative polymerase chain reaction (qPCR), nucleic acid sequencing, ligase chain polymerase chain reaction (LCR-PCR), reverse transcription PCR reaction (RT-PCR), reverse transcription, and nucleic acid ligation.
  • qPCR quantitative polymerase chain reaction
  • LCR-PCR ligase chain polymerase chain reaction
  • RT-PCR reverse transcription PCR reaction
  • nucleic acid ligation including but not limited to nucleic acid amplification reaction that encompasses PCR, quantitative polymerase chain reaction (qPCR), nucleic acid sequencing, ligase chain polymerase chain reaction (LCR-PCR), reverse transcription PCR reaction (RT-PCR), reverse transcription, and nucleic acid ligation.
  • FIG. 21 depicts the SYBR Green staining of G6PDH gene products appeared at the three different thermal stages of one PCR cycle. The three stages are primer annealing at 45° C., denaturation of DNA at 95° C., and primer-dependent extension at 72° C.
  • a “primer” is a short polynucleotide, generally with a free 3′-OH group, that binds to a target nucleic acid (or template) potentially present in a sample of interest by hybridizing with the target nucleic acid, and thereafter promoting polymerization of a polynucleotide complementary to the target.
  • any thermo-controllable units in the upper and/or the bottom arrays may be sealed or unsealed.
  • any thermo-controllable units within the upper and/or the bottom arrays may be filled or unfilled, with or without the reaction sample. Leaving one or more thermo-controllable units unfilled enhances heat insulation because air is a poor conductor of heat. Accordingly, it is preferable to leave the adjacent thermo-controllable units empty so as to reduce heat transfer from one thermo-controllable unit to the next unit. It is also preferable to leave the entire upper or the bottom array of thermo-controllable units empty in order to minimize heat transfer from one layer to the next.
  • Micro-heaters are typically made of materials having high thermal conductivity and chemical stability. Such materials include but are not limited to metals such as chromium, platinum and gold, and semi-conductors such as ceramic, silicon, and geranium.
  • a material particularly suitable for fabricating the micro-heaters is indium tin oxide (ITO). ITO is a transparent ceramic material with a very high electrical conductivity. Because ITO can be prepared in bulk or in form of thin layer, it is particularly useful as either an integral or an external heating element.
  • the temperature sensor may be included as a detachable unit located adjacent to or at the base of the thermo-controllable unit. It can also be integrated into the interior or the exterior surface of the unit. Furthermore, the temperature sensor can be fabricated as an integral part of the micro-heater.
  • This configuration is particularly suited for a range of spectroscopic applications because it permits the application of a wide range of excitation wavelengths to a reaction sample being examined.
  • the configuration supports analyses of fluorescence, chemiluminescence, scintillation, bioluminescence, and time-resolved applications without the need for frequent re-alignment of the excitation sources that provide the appropriate excitation wavelengths.
  • Non-limiting examples of this category of biological entities include RNA viruses such as HIV and hepatitis-causing viruses.
  • Another important application of RT-PCR embodied by the present invention is the simultaneous quantification of biological entities based on the mRNA level detected in the test sample.
  • One of skill in the art will appreciate that if a quantitative result is desired, caution must be taken to use a method that maintains or controls for the relative copies of the amplified nucleic acids.

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  • Wood Science & Technology (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Clinical Laboratory Science (AREA)
  • General Engineering & Computer Science (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Manufacturing & Machinery (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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US11/136,841 2004-05-28 2005-05-24 Chips for multiplex analyses Abandoned US20060030036A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US11/136,841 US20060030036A1 (en) 2004-05-28 2005-05-24 Chips for multiplex analyses

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US85755204A 2004-05-28 2004-05-28
US63078904P 2004-11-24 2004-11-24
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US11/137,263 Expired - Fee Related US7311794B2 (en) 2004-05-28 2005-05-24 Methods of sealing micro wells
US11/137,305 Abandoned US20060030037A1 (en) 2004-05-28 2005-05-24 Thermo-controllable high-density chips for multiplex analyses
US12/624,399 Expired - Lifetime US9228933B2 (en) 2004-05-28 2009-11-23 Apparatus and method for multiplex analysis
US14/857,379 Expired - Lifetime US9909171B2 (en) 2004-05-28 2015-09-17 Thermo-controllable high-density chips for multiplex analyses
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US11/137,263 Expired - Fee Related US7311794B2 (en) 2004-05-28 2005-05-24 Methods of sealing micro wells
US11/137,305 Abandoned US20060030037A1 (en) 2004-05-28 2005-05-24 Thermo-controllable high-density chips for multiplex analyses
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060030037A1 (en) * 2004-05-28 2006-02-09 Victor Joseph Thermo-controllable high-density chips for multiplex analyses
US20080176290A1 (en) * 2007-01-22 2008-07-24 Victor Joseph Apparatus for high throughput chemical reactions
US10641703B2 (en) * 2016-01-07 2020-05-05 University Of Shanghai For Science And Technology Method of enhancing absorption spectral signals of biological samples in terahertz wavebands
US10641772B2 (en) 2015-02-20 2020-05-05 Takara Bio Usa, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
US11460405B2 (en) 2016-07-21 2022-10-04 Takara Bio Usa, Inc. Multi-Z imaging and dispensing with multi-well devices

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
EP2402089A1 (fr) 2003-07-31 2012-01-04 Handylab, Inc. Traitement d'échantillons contenant des particules
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US7429923B2 (en) * 2004-10-22 2008-09-30 Honeywell International Inc. Neuronal sensor networks
EP1812594B1 (fr) * 2004-11-18 2013-01-09 Eppendorf Array Technologies Quantification en temps reel de cibles multiples sur un microreseau
EP1659183A1 (fr) 2004-11-18 2006-05-24 Eppendorf Array Technologies Détèction quantitative en temps reél d'une pluralité de molécules cibles à l'aide d'une bio-puce
JP2006210402A (ja) * 2005-01-25 2006-08-10 Matsushita Electric Ind Co Ltd 半導体装置
US20080275653A1 (en) * 2005-09-14 2008-11-06 Symyx Technologies, Inc. Microscale Flash Separation of Fluid Mixtures
WO2007092571A2 (fr) * 2006-02-07 2007-08-16 Wafergen, Inc. Plaques de culture à régulation thermique
US8288157B2 (en) * 2007-09-12 2012-10-16 Plc Diagnostics, Inc. Waveguide-based optical scanning systems
US9423397B2 (en) 2006-03-10 2016-08-23 Indx Lifecare, Inc. Waveguide-based detection system with scanning light source
US9976192B2 (en) 2006-03-10 2018-05-22 Ldip, Llc Waveguide-based detection system with scanning light source
US9528939B2 (en) 2006-03-10 2016-12-27 Indx Lifecare, Inc. Waveguide-based optical scanning systems
EP1999272B1 (fr) * 2006-03-21 2017-11-01 Koninklijke Philips N.V. Dispositif de detection microelectronique a reseau de capteurs
ITTO20060216A1 (it) * 2006-03-22 2007-09-23 Consiglio Nazionale Ricerche Procedimento per la realizzazione di dispositivi fotonici riscrivibili
ES2692380T3 (es) 2006-03-24 2018-12-03 Handylab, Inc. Método para realizar PCR con un cartucho con varias pistas
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US11806718B2 (en) * 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
EP2002241A1 (fr) * 2006-03-28 2008-12-17 Koninklijke Philips Electronics N.V. Dispositif integre comportant une matrice de photodetecteurs et une matrice de sites d'echantillon
TWI275416B (en) * 2006-04-11 2007-03-11 Touch Micro System Tech Micro sample heating apparatus and method of making the same
CN100491971C (zh) * 2006-06-07 2009-05-27 中国科学院上海微系统与信息技术研究所 基于闪耀光栅和热堆探测器的微型集成光栅光谱仪及制作方法
EP2046940A4 (fr) * 2006-06-26 2011-08-10 Life Technologies Corp Procédés et technologie de couvercle chauffé
WO2008061165A2 (fr) 2006-11-14 2008-05-22 Handylab, Inc. Cartouche microfluidique et son procédé de fabrication
CN101589303B (zh) 2007-01-17 2012-01-11 霍尼韦尔国际公司 基于倏逝波检测的微阵列读取器和读取微阵列的方法
US9475051B2 (en) * 2007-02-27 2016-10-25 Sony Corporation Nucleic acid amplifier
WO2008117210A1 (fr) * 2007-03-23 2008-10-02 Koninklijke Philips Electronics N.V. Dispositif microfluidique intégré à contrôle local de température
WO2008118500A1 (fr) * 2007-03-27 2008-10-02 Wafergen, Inc. Plaque de perfusion de substance nutritive avec chauffage et échange de gaz pour criblage de contenu élevé
JP5205802B2 (ja) * 2007-05-11 2013-06-05 ソニー株式会社 リアルタイムｐｃｒ装置
JP5045268B2 (ja) 2007-06-28 2012-10-10 ソニー株式会社 反応処理装置
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
WO2009012185A1 (fr) 2007-07-13 2009-01-22 Handylab, Inc. Matières absorbant les polynucléotides, et procédés d'utilisation de celles-ci
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
WO2009019658A2 (fr) * 2007-08-09 2009-02-12 Koninklijke Philips Electronics N.V. Dispositif microfluidique intégré avec commande de température local
US20090165574A1 (en) * 2007-12-27 2009-07-02 Finnzymes Instruments Oy Instrument and method for nucleic acid amplification
WO2009101584A2 (fr) * 2008-02-15 2009-08-20 Koninklijke Philips Electronics N.V. Système de commande pour cartouche de laboratoire sur puce
JP4544335B2 (ja) * 2008-04-15 2010-09-15 ソニー株式会社 反応処理装置
JP2009254260A (ja) * 2008-04-15 2009-11-05 Sony Corp 反応処理装置
GB2461026B (en) * 2008-06-16 2011-03-09 Plc Diagnostics Inc System and method for nucleic acids sequencing by phased synthesis
EP2364219B1 (fr) * 2008-12-05 2018-10-31 Biocartis NV Système de cyclage en température comprenant une unité de chauffage transparente
EP2584344B1 (fr) 2009-01-08 2021-06-30 IT-IS International Limited Système optique pour détecter la lumière des réactions de polymérase en chaîne
EP2233577A1 (fr) * 2009-03-20 2010-09-29 F. Hoffmann-La Roche AG Procédé et appareil pour tester l'activité cancérigène potentielle de substances
WO2010127001A1 (fr) * 2009-04-29 2010-11-04 Plc Diagnostics Inc. Système de détection à guide d'ondes à source de lumière à balayage
JP5310373B2 (ja) * 2009-05-14 2013-10-09 ソニー株式会社 光学的検出装置
GB2483402B (en) 2009-06-04 2014-04-09 Lockheed Corp Multiple-sample microfluidic chip for DNA analysis
CN102039639A (zh) * 2009-10-14 2011-05-04 龙生工业股份有限公司 塑料成型辅助系统
US8809810B2 (en) 2010-05-20 2014-08-19 Honeywell International Inc. Microarray reader based on evanescent wave detection
BE1019409A5 (nl) * 2010-07-07 2012-07-03 Octonus Finland Oy Verbeterde werkwijze voor het analyseren van een edelsteen.
CA2814720C (fr) 2010-10-15 2016-12-13 Lockheed Martin Corporation Conception optique microfluidique
CA2821580A1 (fr) 2010-12-17 2012-06-21 Bjs Ip Limited Procedes et systemes pour chauffage de pcr rapide
CN106148512B (zh) 2011-04-15 2020-07-10 贝克顿·迪金森公司 扫描实时微流体热循环仪和用于同步的热循环和扫描光学检测的方法
WO2012178210A1 (fr) * 2011-06-23 2012-12-27 Anitoa Systems, Llc Appareil destiné à l'amplification d'acides nucléiques
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
JP6262152B2 (ja) 2012-02-03 2018-01-17 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 分子診断試験の分布及び試験間のコンパチビリティ判断のための外部ファイル
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
US9579657B2 (en) 2012-05-24 2017-02-28 Bjs Ip Ltd Clamp for fast PCR heating
AU2013202793B2 (en) 2012-07-31 2014-09-18 Gen-Probe Incorporated System, method and apparatus for automated incubation
US9447925B2 (en) 2012-11-08 2016-09-20 Wafergen, Inc. Extraction of restrained liquid from wells
US9006845B2 (en) * 2013-01-16 2015-04-14 Infineon Technologies, A.G. MEMS device with polymer layer, system of a MEMS device with a polymer layer, method of making a MEMS device with a polymer layer
ES2878525T3 (es) 2013-03-13 2021-11-19 Illumina Inc Dispositivos fluídicos multicapa y procedimientos para su fabricación
WO2015116627A1 (fr) 2014-01-29 2015-08-06 Arizona Board Of Regents On Behalf Of Arizona State University Plateforme d'ensemble de micro-réacteurs
US10018566B2 (en) 2014-02-28 2018-07-10 Ldip, Llc Partially encapsulated waveguide based sensing chips, systems and methods of use
EP3155396A4 (fr) 2014-06-12 2017-12-27 Wafergen, Inc. Capture de cellules individuelles avec des films de capture en polymère
US9757707B2 (en) 2014-06-12 2017-09-12 Takara Bio Usa, Inc. Single cell capture with capture chips
US10208339B2 (en) 2015-02-19 2019-02-19 Takara Bio Usa, Inc. Systems and methods for whole genome amplification
US11181479B2 (en) 2015-02-27 2021-11-23 Ldip, Llc Waveguide-based detection system with scanning light source
CN114015569A (zh) 2015-04-21 2022-02-08 通用自动化实验技术公司 用于高通量微生物学应用的高分辨率系统、试剂盒、设备和方法
JP6239562B2 (ja) * 2015-09-14 2017-11-29 株式会社東芝 照明デバイスおよびそれを備えるバイオ情報計測装置
TWI565945B (zh) * 2015-12-11 2017-01-11 台灣奈米碳素股份有限公司 一種利用氣體辨識而具肺炎感染與肺炎菌種疾病分析功能的呼吸器
US11371087B2 (en) 2016-06-10 2022-06-28 Takara Bio Usa, Inc. Methods and compositions employing blocked primers
US10570015B2 (en) * 2016-09-02 2020-02-25 International Business Machines Corporation Minimizing tin loss during thermal processing of kesterite films
JP7050057B2 (ja) 2016-11-10 2022-04-07 タカラ バイオ ユーエスエー， インコーポレイテッド 増幅二本鎖デオキシリボ核酸の作製方法ならびに該方法で使用する組成物及びキット
US20180172906A1 (en) * 2016-12-16 2018-06-21 Quantum-Si Incorporated Optical coupler and waveguide system
CN110184325A (zh) * 2018-02-22 2019-08-30 张家港万众一芯生物科技有限公司 基于微孔阵列芯片的单分子文库pcr扩增的基因测序方法
DE102018111033A1 (de) * 2018-05-08 2019-11-14 Byonoy Gmbh Transmissionsvorrichtung zur Untersuchung von Proben in Kavitäten einer Mikrotiterplatte und Verfahren zum Untersuchen von Proben in Kavitäten einer Mikrotiterplatte mittels Transmission
CN108614600B (zh) * 2018-07-13 2024-04-30 中国科学院天津工业生物技术研究所 一种高精度芯片反应系统及方法
US11666910B2 (en) 2018-11-26 2023-06-06 Hewlett-Packard Development Company, L.P. Microfluidic devices
CN111154644B (zh) * 2020-01-17 2023-02-10 北京双诚联盈净化工程技术有限公司 一种聚合酶链式反应扩增全自动工作站
CN111289440A (zh) * 2020-03-02 2020-06-16 武汉铢寸科技有限公司 一种自加热防雾生物光学微孔阵列板
DE102020108957B4 (de) 2020-03-31 2021-10-07 Otto-Von-Guericke-Universität Magdeburg Vorrichtung, Verfahren und Computerprogramm zur Fluoreszenzmessung
US11378444B2 (en) * 2020-08-05 2022-07-05 Visera Technologies Company Limited Biosensor and bio detection system
CN111975887A (zh) * 2020-09-09 2020-11-24 浙江品阁木业有限公司 一种基于无机纳米粒子改性smf树脂涂层的实木复合地板
WO2023037334A1 (fr) 2021-09-13 2023-03-16 Ecole Polytechnique Federale De Lausanne (Epfl) Système et procédé de profilage phénotypique de cellule unique et d'encapsulation de gouttelettes de l'ordre du nanolitre déterministe et ensembles de consortiums de gouttelettes déterministes

Citations (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5252294A (en) * 1988-06-01 1993-10-12 Messerschmitt-Bolkow-Blohm Gmbh Micromechanical structure
US5475610A (en) * 1990-11-29 1995-12-12 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5552321A (en) * 1993-08-24 1996-09-03 Bioptechs Inc. Temperature controlled culture dish apparatus
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5589136A (en) * 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
US5593838A (en) * 1994-11-10 1997-01-14 David Sarnoff Research Center, Inc. Partitioned microelectronic device array
US5639423A (en) * 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US5674743A (en) * 1993-02-01 1997-10-07 Seq, Ltd. Methods and apparatus for DNA sequencing
US5716842A (en) * 1994-09-30 1998-02-10 Biometra Biomedizinische Analytik Gmbh Miniaturized flow thermocycler
US5866345A (en) * 1992-05-01 1999-02-02 The Trustees Of The University Of Pennsylvania Apparatus for the detection of an analyte utilizing mesoscale flow systems
US5939312A (en) * 1995-05-24 1999-08-17 Biometra Biomedizinische Analytik Gmbh Miniaturized multi-chamber thermocycler
US5985555A (en) * 1994-03-19 1999-11-16 Boehringer Mannheim Gmbh Method and apparatus for processing nucleic acids using a small temperature-changing zone
US6015674A (en) * 1994-04-29 2000-01-18 Perkin-Elmer Corporation Applied Biosystems Division Apparatus and method for detecting nucleic acid amplification products
US6126804A (en) * 1997-09-23 2000-10-03 The Regents Of The University Of California Integrated polymerase chain reaction/electrophoresis instrument
US6132580A (en) * 1995-09-28 2000-10-17 The Regents Of The University Of California Miniature reaction chamber and devices incorporating same
US6174675B1 (en) * 1997-11-25 2001-01-16 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6184029B1 (en) * 1992-05-01 2001-02-06 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US6197572B1 (en) * 1998-05-04 2001-03-06 Roche Diagnostics Corporation Thermal cycler having an automatically positionable lid
US6303343B1 (en) * 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6306590B1 (en) * 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
US6309886B1 (en) * 1999-06-04 2001-10-30 The Regents Of The University Of California High throughput analysis of samples in flowing liquid
US20020060156A1 (en) * 1998-12-28 2002-05-23 Affymetrix, Inc. Integrated microvolume device
US20020068357A1 (en) * 1995-09-28 2002-06-06 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
US20020072113A1 (en) * 2000-12-07 2002-06-13 Emilio Barbera-Guillem Microincubator comprising a cell culture apparatus and a transparent heater
US6406893B1 (en) * 1997-04-04 2002-06-18 Caliper Technologies Corp. Microfluidic methods for non-thermal nucleic acid manipulations
US6420143B1 (en) * 1998-02-13 2002-07-16 Caliper Technologies Corp. Methods and systems for performing superheated reactions in microscale fluidic systems
US6423948B1 (en) * 2000-12-12 2002-07-23 3-Dimensional Pharmaceuticals, Inc. Microtiter plate with integral heater
US6423536B1 (en) * 1999-08-02 2002-07-23 Molecular Dynamics, Inc. Low volume chemical and biochemical reaction system
US6432695B1 (en) * 2001-02-16 2002-08-13 Institute Of Microelectronics Miniaturized thermal cycler
US6444461B1 (en) * 1997-04-04 2002-09-03 Caliper Technologies Corp. Microfluidic devices and methods for separation
US20020144771A1 (en) * 1999-02-11 2002-10-10 Kuczynski Joseph Paul UV curable adhesives containing ceramic microspheres
US20020182544A1 (en) * 2000-01-11 2002-12-05 Sipix Imaging, Inc. Process for roll-to-roll manufacture of a display by synchronized photolithographic exposure on a substrate web
US6503750B1 (en) * 1998-11-25 2003-01-07 The Regents Of The University Of California PCR thermocycler
US20030006003A1 (en) * 2001-06-29 2003-01-09 Hideki Matsuoka Method for manufacturing display panel
US6509186B1 (en) * 2001-02-16 2003-01-21 Institute Of Microelectronics Miniaturized thermal cycler
US6524532B1 (en) * 1995-06-20 2003-02-25 The Regents Of The University Of California Microfabricated sleeve devices for chemical reactions
US20030040011A1 (en) * 2001-08-24 2003-02-27 Barth Phillip W. Apparatus and method for simultaneously conducting multiple chemical reactions
US6537799B2 (en) * 1997-09-02 2003-03-25 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6541274B2 (en) * 1999-03-08 2003-04-01 Caliper Technologies Corp. Integrated devices and method of use for performing temperature controlled reactions and analyses
US6576459B2 (en) * 2001-03-23 2003-06-10 The Regents Of The University Of California Sample preparation and detection device for infectious agents
US6586233B2 (en) * 2001-03-09 2003-07-01 The Regents Of The University Of California Convectively driven PCR thermal-cycling
US20030138829A1 (en) * 2001-11-30 2003-07-24 Fluidigm Corp. Microfluidic device and methods of using same
US20030199081A1 (en) * 1992-05-01 2003-10-23 Peter Wilding Mesoscale polynucleotide amplification analysis
US6656724B1 (en) * 1997-11-19 2003-12-02 Mwg-Biotech Ag Apparatus for automatic implementation of chemical or biological methods
US6670153B2 (en) * 2000-09-14 2003-12-30 Caliper Technologies Corp. Microfluidic devices and methods for performing temperature mediated reactions
US6699713B2 (en) * 2000-01-04 2004-03-02 The Regents Of The University Of California Polymerase chain reaction system
US6703236B2 (en) * 1990-11-29 2004-03-09 Applera Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
US20040072334A1 (en) * 2002-10-15 2004-04-15 The Regents Of The University Of California Thermal cycler
US20040096958A1 (en) * 2002-03-05 2004-05-20 Raveendran Pottathil Thermal strip thermocycler
US6756019B1 (en) * 1998-02-24 2004-06-29 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
US6762049B2 (en) * 2001-07-05 2004-07-13 Institute Of Microelectronics Miniaturized multi-chamber thermal cycler for independent thermal multiplexing
US6814934B1 (en) * 1991-05-02 2004-11-09 Russell Gene Higuchi Instrument for monitoring nucleic acid amplification
US6830936B2 (en) * 1995-06-29 2004-12-14 Affymetrix Inc. Integrated nucleic acid diagnostic device
US20050019792A1 (en) * 2001-11-30 2005-01-27 Fluidigm Corporation Microfluidic device and methods of using same
US20050112634A1 (en) * 2003-09-19 2005-05-26 Woudenberg Timothy M. High density sequence detection methods and apparatus
US20050129581A1 (en) * 2003-04-03 2005-06-16 Fluidigm Corporation Microfluidic devices and methods of using same
US20050145273A1 (en) * 1997-03-28 2005-07-07 Atwood John G. Thermal cycler for PCR
US20050176155A1 (en) * 2004-02-11 2005-08-11 Gener8, Inc. Well plate reactor
US20050225751A1 (en) * 2003-09-19 2005-10-13 Donald Sandell Two-piece high density plate
US20060030037A1 (en) * 2004-05-28 2006-02-09 Victor Joseph Thermo-controllable high-density chips for multiplex analyses
US7164077B2 (en) * 2001-04-09 2007-01-16 Research Triangle Institute Thin-film thermoelectric cooling and heating devices for DNA genomic and proteomic chips, thermo-optical switching circuits, and IR tags

Family Cites Families (130)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5656493A (en) 1985-03-28 1997-08-12 The Perkin-Elmer Corporation System for automated performance of the polymerase chain reaction
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US7560273B2 (en) 2002-07-23 2009-07-14 Applied Biosystems, Llc Slip cover for heated platen assembly
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5281516A (en) * 1988-08-02 1994-01-25 Gene Tec Corporation Temperature control apparatus and method
ES2091225T3 (es) 1989-07-11 1996-11-01 Gen Probe Inc Metodos para la amplificacion de las secuencias de acidos nucleicos.
CA2020958C (fr) 1989-07-11 2005-01-11 Daniel L. Kacian Methodes d'amplification de sequences d'acide nucleique
CA2031912A1 (fr) 1989-12-22 1991-06-23 Robert Fred Pfost Couvercle chauffe
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US6787338B2 (en) 1990-06-04 2004-09-07 The University Of Utah Method for rapid thermal cycling of biological samples
US5225300A (en) * 1990-11-28 1993-07-06 Mita Industrial Co., Ltd. Electrophotographic toner for negative charging
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
AU677781B2 (en) 1992-05-01 1997-05-08 Trustees Of The University Of Pennsylvania, The Microfabricated sperm handling devices
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5342581A (en) 1993-04-19 1994-08-30 Sanadi Ashok R Apparatus for preventing cross-contamination of multi-well test plates
EP0636413B1 (fr) * 1993-07-28 2001-11-14 PE Corporation (NY) Dispositif et procédé d'amplification d'acide nucléique
US5525300A (en) * 1993-10-20 1996-06-11 Stratagene Thermal cycler including a temperature gradient block
US5714330A (en) 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
EP0777749B1 (fr) 1994-08-19 2002-10-30 PE Corporation (NY) Procede de ligature et d'amplification associees
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5721136A (en) 1994-11-09 1998-02-24 Mj Research, Inc. Sealing device for thermal cycling vessels
CA2181189C (fr) 1994-11-14 1999-09-21 Peter Wilding Dispositifs d'amplification de polynucleotides a meso-echelle
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
WO1997007390A1 (fr) 1995-08-21 1997-02-27 Foss Electric A/S Procede et appareil pour compter les particules ou cellules dans un echantillon de liquide
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
WO1997024616A1 (fr) 1995-12-29 1997-07-10 Ishihara Sangyo Kaisha, Ltd. Procede de determination du taux de viabilite de cellules
AU741049B2 (en) 1996-05-09 2001-11-22 Life Technologies Corporation Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
DE29623597U1 (de) * 1996-11-08 1999-01-07 Eppendorf - Netheler - Hinz Gmbh, 22339 Hamburg Temperierblock mit Temperiereinrichtungen
US6458584B1 (en) * 1996-12-23 2002-10-01 University Of Chicago Customized oligonucleotide microchips that convert multiple genetic information to simple patterns, are portable and reusable
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6548263B1 (en) * 1997-05-29 2003-04-15 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
EP1028970A1 (fr) 1997-10-10 2000-08-23 President And Fellows Of Harvard College Amplification par replique de reseaux d'acides nucleiques
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
ATE363339T1 (de) * 1998-05-01 2007-06-15 Gen Probe Inc Rührvorrichtung für den fluiden inhalt eines behälters
US6818437B1 (en) 1998-05-16 2004-11-16 Applera Corporation Instrument for monitoring polymerase chain reaction of DNA
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
CA2255850C (fr) 1998-12-07 2000-10-17 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Appareil de thermocyclage rotatif
DE19859586C1 (de) 1998-12-22 2000-07-13 Mwg Biotech Ag Thermocyclervorrichtung
US6225061B1 (en) 1999-03-10 2001-05-01 Sequenom, Inc. Systems and methods for performing reactions in an unsealed environment
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6657169B2 (en) 1999-07-30 2003-12-02 Stratagene Apparatus for thermally cycling samples of biological material with substantial temperature uniformity
US6713309B1 (en) 1999-07-30 2004-03-30 Large Scale Proteomics Corporation Microarrays and their manufacture
US6337435B1 (en) * 1999-07-30 2002-01-08 Bio-Rad Laboratories, Inc. Temperature control for multi-vessel reaction apparatus
AU7537200A (en) 1999-09-29 2001-04-30 Solexa Ltd. Polynucleotide sequencing
DE29917313U1 (de) 1999-10-01 2001-02-15 MWG-BIOTECH AG, 85560 Ebersberg Vorrichtung zur Durchführung chemischer oder biologischer Reaktionen
US7332271B2 (en) 2000-02-18 2008-02-19 Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for parallel processing of micro-volume liquid reactions
US6489094B2 (en) * 2000-05-31 2002-12-03 Pfizer Inc. Method and device for drug-drug interaction testing sample preparation
JP2002010777A (ja) 2000-06-30 2002-01-15 Precision System Science Co Ltd 反応容器、反応装置および反応液の温度制御方法
CN101525660A (zh) 2000-07-07 2009-09-09 维西根生物技术公司 实时序列测定
US6706891B2 (en) * 2000-11-06 2004-03-16 Eastman Chemical Company Process for the preparation of ligands for olefin polymerization catalysts
GB2370112A (en) 2000-12-15 2002-06-19 Hybaid Ltd Multiwell sample plates
US7668697B2 (en) 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US20040209331A1 (en) 2001-07-16 2004-10-21 Kirk Ririe Thermal cycling system and method of use
US6881579B2 (en) * 2001-07-30 2005-04-19 Agilent Technologies, Inc. Sample processing apparatus and methods
US6673595B2 (en) * 2001-08-27 2004-01-06 Biocrystal, Ltd Automated cell management system for growth and manipulation of cultured cells
US6682703B2 (en) 2001-09-05 2004-01-27 Irm, Llc Parallel reaction devices
JP3696141B2 (ja) 2001-09-27 2005-09-14 株式会社東芝 化学分析装置、分析方法
US7338760B2 (en) 2001-10-26 2008-03-04 Ntu Ventures Private Limited Sample preparation integrated chip
US6677151B2 (en) * 2002-01-30 2004-01-13 Applera Corporation Device and method for thermal cycling
WO2003068918A2 (fr) 2002-02-11 2003-08-21 Auburn University Reaction en chaine polymerase en temps reel a sensibilite elevee pour detecter des acides nucleiques
AUPS205802A0 (en) 2002-05-01 2002-06-06 Bio-Molecular Holdings Pty Limited Improved cycling device and method
DE10221763A1 (de) 2002-05-15 2003-12-04 Eppendorf Ag Thermocycler mit in Cyclen angesteuertem Temperierblock
US7007001B2 (en) * 2002-06-26 2006-02-28 Microsoft Corporation Maximizing mutual information between observations and hidden states to minimize classification errors
US20060063225A1 (en) 2002-08-01 2006-03-23 Caulfield Michael J Novel procedure for growth, imaging, and enumeration of microbial colonies for serological or screening assays
US6962822B2 (en) * 2002-08-07 2005-11-08 International Business Machines Corporation Discrete nano-textured structures in biomolecular arrays, and method of use
US20040043494A1 (en) * 2002-08-30 2004-03-04 Amorese Douglas A. Apparatus for studying arrays
AU2003270832A1 (en) * 2002-09-24 2004-04-19 U.S. Government As Represented By The Secretary Of The Army Portable thermocycler
US6768084B2 (en) * 2002-09-30 2004-07-27 Axcelis Technologies, Inc. Advanced rapid thermal processing (RTP) using a linearly-moving heating assembly with an axisymmetric and radially-tunable thermal radiation profile
US6730883B2 (en) 2002-10-02 2004-05-04 Stratagene Flexible heating cover assembly for thermal cycling of samples of biological material
US7017592B2 (en) * 2002-12-10 2006-03-28 Pro-Environmental Inc. Regenerative fume-incinerator with on-line burn-out and wash-down system
US20060094108A1 (en) 2002-12-20 2006-05-04 Karl Yoder Thermal cycler for microfluidic array assays
DE602004036672C5 (de) 2003-01-29 2012-11-29 454 Life Sciences Corporation Nukleinsäureamplifikation auf Basis von Kügelchenemulsion
AU2004243070B2 (en) * 2003-05-23 2010-04-15 Bio-Rad Laboratories, Inc. Localized temperature control for spatial arrays of reaction media
DE10325300A1 (de) * 2003-06-04 2005-01-20 Siemens Ag Thermocycler
ES2265199B1 (es) 2003-06-12 2008-02-01 Cellerix, S.L. Celulas madre adultas multipotentes procedentes de condrocitos desdiferenciados y sus aplicaciones.
US7417726B2 (en) 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
EP1670945A2 (fr) 2003-09-19 2006-06-21 Applera Corporation Microplaques utilisees pour effectuer une amplification nucleotidique par cyclage thermique
US7460223B2 (en) 2003-09-19 2008-12-02 Applied Biosystems Inc. Inverted orientation for a microplate
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
DE102004022263A1 (de) 2004-05-06 2005-12-15 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
US7585663B2 (en) * 2004-08-26 2009-09-08 Applied Biosystems, Llc Thermal device, system, and method, for fluid processing device
CA2957197C (fr) 2004-08-27 2017-12-12 Gen-Probe Incorporated Techniques d'amplification d'acide nucleique avec une seule amorce
US7051536B1 (en) 2004-11-12 2006-05-30 Bio-Rad Laboratories, Inc. Thermal cycler with protection from atmospheric moisture
US7659110B2 (en) 2005-01-21 2010-02-09 Thermogen Inc. DNA amplification device
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
EP2272983A1 (fr) 2005-02-01 2011-01-12 AB Advanced Genetic Analysis Corporation Réactifs, méthodes et bibliothèques pour séquencage fondé sur des billes
EP1693337A1 (fr) 2005-02-18 2006-08-23 Infineon Technologies AG Support macroporeux pour réactions d'amplification
EP2518161A1 (fr) 2005-03-18 2012-10-31 Fluidigm Corporation Procédé de detection d'allèles mutants
US20060246493A1 (en) 2005-04-04 2006-11-02 Caliper Life Sciences, Inc. Method and apparatus for use in temperature controlled processing of microfluidic samples
US7883669B2 (en) 2005-04-20 2011-02-08 Fluidigm Corporation Analysis engine and database for manipulating parameters for fluidic systems on a chip
WO2006124512A2 (fr) 2005-05-13 2006-11-23 Applera Corporation Bloc de cyclage thermique de faible poids
US7117726B1 (en) * 2005-06-17 2006-10-10 Gm Global Technology Operations, Inc. Method and apparatus for indirect measurement of engine cylinder pressure
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
TWI310890B (en) * 2005-10-13 2009-06-11 Ind Tech Res Inst Apparatus for controlling fluid temperature and method thereof
US7282337B1 (en) 2006-04-14 2007-10-16 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US20080026483A1 (en) * 2006-06-14 2008-01-31 Oldenburg Kevin R Thermal-cycling devices and methods of using the same
US20070290282A1 (en) 2006-06-15 2007-12-20 Nanochip, Inc. Bonded chip assembly with a micro-mover for microelectromechanical systems
US20080241951A1 (en) 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Method and apparatus for moving stage detection of single molecular events
US20080240542A1 (en) 2006-10-03 2008-10-02 Kalypsys, Inc. Droplet detection system
EP2653861B1 (fr) 2006-12-14 2014-08-13 Life Technologies Corporation Procédé pour le séquençage d'un acide nucléique en utilisant des matrices de FET à grande échelle
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
WO2008091626A1 (fr) 2007-01-22 2008-07-31 Wafergen, Inc. Appareil permettant la mise en oeuvre de réactions chimiques à haut rendement
US20090061513A1 (en) 2007-05-15 2009-03-05 Picovitro Ab Cell sorting and cell cultivation methods
AU2008265691B2 (en) 2007-06-19 2014-04-24 F. Hoffmann-La Roche Ag High throughput nucleic acid sequencing by expansion
GB2468993A (en) 2007-12-27 2010-09-29 Finnzymes Instr Oy Thermal cycling instrument and methods for carrying out pcr
WO2009100933A1 (fr) 2008-02-15 2009-08-20 Eppendorf Ag Dispositif thermique
US9097702B2 (en) 2008-07-29 2015-08-04 Cornell University Pathologically relevant tumor microenvironments for high-throughput drug screening
WO2010022391A2 (fr) 2008-08-22 2010-02-25 Azte Arizona Technology Enterprises Système automatisé et intégré pour l'étude de fonctions cellulaires et tissulaires
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
DK2414548T3 (en) 2009-03-30 2015-12-21 Illumina Inc Gene expression in single cells
GB0909420D0 (en) 2009-06-02 2009-07-15 Biochip Devises Pte Ltd Device for nucleic acid amplification
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
ES2523140T3 (es) 2010-09-21 2014-11-21 Population Genetics Technologies Ltd. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
US10392726B2 (en) 2010-10-08 2019-08-27 President And Fellows Of Harvard College High-throughput immune sequencing
GB201213654D0 (en) 2012-08-01 2012-09-12 Univ Singapore Cell culture
US9447925B2 (en) 2012-11-08 2016-09-20 Wafergen, Inc. Extraction of restrained liquid from wells
US20160122753A1 (en) 2013-06-12 2016-05-05 Tarjei Mikkelsen High-throughput rna-seq
TW201446428A (zh) 2013-06-15 2014-12-16 Saint Gobain Abrasives Inc 研磨工具及其形成方法
KR102536833B1 (ko) 2013-08-28 2023-05-26 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석

Patent Citations (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5252294A (en) * 1988-06-01 1993-10-12 Messerschmitt-Bolkow-Blohm Gmbh Micromechanical structure
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5602756A (en) * 1990-11-29 1997-02-11 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
US5475610A (en) * 1990-11-29 1995-12-12 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
US6703236B2 (en) * 1990-11-29 2004-03-09 Applera Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
US6814934B1 (en) * 1991-05-02 2004-11-09 Russell Gene Higuchi Instrument for monitoring nucleic acid amplification
US20030199081A1 (en) * 1992-05-01 2003-10-23 Peter Wilding Mesoscale polynucleotide amplification analysis
US6660517B1 (en) * 1992-05-01 2003-12-09 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US6184029B1 (en) * 1992-05-01 2001-02-06 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US6551841B1 (en) * 1992-05-01 2003-04-22 The Trustees Of The University Of Pennsylvania Device and method for the detection of an analyte utilizing mesoscale flow systems
US5866345A (en) * 1992-05-01 1999-02-02 The Trustees Of The University Of Pennsylvania Apparatus for the detection of an analyte utilizing mesoscale flow systems
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5955029A (en) * 1992-05-01 1999-09-21 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5639423A (en) * 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US5674743A (en) * 1993-02-01 1997-10-07 Seq, Ltd. Methods and apparatus for DNA sequencing
US5552321A (en) * 1993-08-24 1996-09-03 Bioptechs Inc. Temperature controlled culture dish apparatus
US5985555A (en) * 1994-03-19 1999-11-16 Boehringer Mannheim Gmbh Method and apparatus for processing nucleic acids using a small temperature-changing zone
US6015674A (en) * 1994-04-29 2000-01-18 Perkin-Elmer Corporation Applied Biosystems Division Apparatus and method for detecting nucleic acid amplification products
US5716842A (en) * 1994-09-30 1998-02-10 Biometra Biomedizinische Analytik Gmbh Miniaturized flow thermocycler
US5593838A (en) * 1994-11-10 1997-01-14 David Sarnoff Research Center, Inc. Partitioned microelectronic device array
US5939312A (en) * 1995-05-24 1999-08-17 Biometra Biomedizinische Analytik Gmbh Miniaturized multi-chamber thermocycler
US6602473B1 (en) * 1995-06-20 2003-08-05 The Regents Of The University Of California Temperature control apparatus
US6524532B1 (en) * 1995-06-20 2003-02-25 The Regents Of The University Of California Microfabricated sleeve devices for chemical reactions
US5589136A (en) * 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
US6830936B2 (en) * 1995-06-29 2004-12-14 Affymetrix Inc. Integrated nucleic acid diagnostic device
US6284525B1 (en) * 1995-09-28 2001-09-04 Affymetrix, Inc. Miniature reaction chamber and devices incorporating same
US6132580A (en) * 1995-09-28 2000-10-17 The Regents Of The University Of California Miniature reaction chamber and devices incorporating same
US20020068357A1 (en) * 1995-09-28 2002-06-06 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
US7133726B1 (en) * 1997-03-28 2006-11-07 Applera Corporation Thermal cycler for PCR
US20050145273A1 (en) * 1997-03-28 2005-07-07 Atwood John G. Thermal cycler for PCR
US6444461B1 (en) * 1997-04-04 2002-09-03 Caliper Technologies Corp. Microfluidic devices and methods for separation
US6406893B1 (en) * 1997-04-04 2002-06-18 Caliper Technologies Corp. Microfluidic methods for non-thermal nucleic acid manipulations
US6537799B2 (en) * 1997-09-02 2003-03-25 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6126804A (en) * 1997-09-23 2000-10-03 The Regents Of The University Of California Integrated polymerase chain reaction/electrophoresis instrument
US6656724B1 (en) * 1997-11-19 2003-12-02 Mwg-Biotech Ag Apparatus for automatic implementation of chemical or biological methods
US6174675B1 (en) * 1997-11-25 2001-01-16 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6420143B1 (en) * 1998-02-13 2002-07-16 Caliper Technologies Corp. Methods and systems for performing superheated reactions in microscale fluidic systems
US6756019B1 (en) * 1998-02-24 2004-06-29 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
US6197572B1 (en) * 1998-05-04 2001-03-06 Roche Diagnostics Corporation Thermal cycler having an automatically positionable lid
US6306590B1 (en) * 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
US6503750B1 (en) * 1998-11-25 2003-01-07 The Regents Of The University Of California PCR thermocycler
US20020060156A1 (en) * 1998-12-28 2002-05-23 Affymetrix, Inc. Integrated microvolume device
US20020144771A1 (en) * 1999-02-11 2002-10-10 Kuczynski Joseph Paul UV curable adhesives containing ceramic microspheres
US6541274B2 (en) * 1999-03-08 2003-04-01 Caliper Technologies Corp. Integrated devices and method of use for performing temperature controlled reactions and analyses
US6524830B2 (en) * 1999-04-06 2003-02-25 Caliper Technologies Corp. Microfluidic devices and systems for performing inefficient fast PCR
US6303343B1 (en) * 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6309886B1 (en) * 1999-06-04 2001-10-30 The Regents Of The University Of California High throughput analysis of samples in flowing liquid
US6423536B1 (en) * 1999-08-02 2002-07-23 Molecular Dynamics, Inc. Low volume chemical and biochemical reaction system
US6699713B2 (en) * 2000-01-04 2004-03-02 The Regents Of The University Of California Polymerase chain reaction system
US20020182544A1 (en) * 2000-01-11 2002-12-05 Sipix Imaging, Inc. Process for roll-to-roll manufacture of a display by synchronized photolithographic exposure on a substrate web
US6670153B2 (en) * 2000-09-14 2003-12-30 Caliper Technologies Corp. Microfluidic devices and methods for performing temperature mediated reactions
US20020072113A1 (en) * 2000-12-07 2002-06-13 Emilio Barbera-Guillem Microincubator comprising a cell culture apparatus and a transparent heater
US6423948B1 (en) * 2000-12-12 2002-07-23 3-Dimensional Pharmaceuticals, Inc. Microtiter plate with integral heater
US6432695B1 (en) * 2001-02-16 2002-08-13 Institute Of Microelectronics Miniaturized thermal cycler
US6509186B1 (en) * 2001-02-16 2003-01-21 Institute Of Microelectronics Miniaturized thermal cycler
US6586233B2 (en) * 2001-03-09 2003-07-01 The Regents Of The University Of California Convectively driven PCR thermal-cycling
US6576459B2 (en) * 2001-03-23 2003-06-10 The Regents Of The University Of California Sample preparation and detection device for infectious agents
US7164077B2 (en) * 2001-04-09 2007-01-16 Research Triangle Institute Thin-film thermoelectric cooling and heating devices for DNA genomic and proteomic chips, thermo-optical switching circuits, and IR tags
US20030006003A1 (en) * 2001-06-29 2003-01-09 Hideki Matsuoka Method for manufacturing display panel
US6762049B2 (en) * 2001-07-05 2004-07-13 Institute Of Microelectronics Miniaturized multi-chamber thermal cycler for independent thermal multiplexing
US20030040011A1 (en) * 2001-08-24 2003-02-27 Barth Phillip W. Apparatus and method for simultaneously conducting multiple chemical reactions
US20050019792A1 (en) * 2001-11-30 2005-01-27 Fluidigm Corporation Microfluidic device and methods of using same
US20030138829A1 (en) * 2001-11-30 2003-07-24 Fluidigm Corp. Microfluidic device and methods of using same
US20040096958A1 (en) * 2002-03-05 2004-05-20 Raveendran Pottathil Thermal strip thermocycler
US20040072334A1 (en) * 2002-10-15 2004-04-15 The Regents Of The University Of California Thermal cycler
US20050129581A1 (en) * 2003-04-03 2005-06-16 Fluidigm Corporation Microfluidic devices and methods of using same
US20050112634A1 (en) * 2003-09-19 2005-05-26 Woudenberg Timothy M. High density sequence detection methods and apparatus
US20050225751A1 (en) * 2003-09-19 2005-10-13 Donald Sandell Two-piece high density plate
US20050176155A1 (en) * 2004-02-11 2005-08-11 Gener8, Inc. Well plate reactor
US20060030035A1 (en) * 2004-05-28 2006-02-09 Victor Joseph Thermo-controllable chips for multiplex analyses
US20060073491A1 (en) * 2004-05-28 2006-04-06 Victor Joseph Apparatus and method for multiplex analysis
US20060027317A1 (en) * 2004-05-28 2006-02-09 Victor Joseph Methods of sealing micro wells
US20060030037A1 (en) * 2004-05-28 2006-02-09 Victor Joseph Thermo-controllable high-density chips for multiplex analyses
US7311794B2 (en) * 2004-05-28 2007-12-25 Wafergen, Inc. Methods of sealing micro wells

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9228933B2 (en) 2004-05-28 2016-01-05 Wafergen, Inc. Apparatus and method for multiplex analysis
US20060030035A1 (en) * 2004-05-28 2006-02-09 Victor Joseph Thermo-controllable chips for multiplex analyses
US20060073491A1 (en) * 2004-05-28 2006-04-06 Victor Joseph Apparatus and method for multiplex analysis
US10718014B2 (en) 2004-05-28 2020-07-21 Takara Bio Usa, Inc. Thermo-controllable high-density chips for multiplex analyses
US7622296B2 (en) 2004-05-28 2009-11-24 Wafergen, Inc. Apparatus and method for multiplex analysis
US20100233698A1 (en) * 2004-05-28 2010-09-16 Wafergen, Inc. Apparatus and method for multiplex analysis
US7833709B2 (en) 2004-05-28 2010-11-16 Wafergen, Inc. Thermo-controllable chips for multiplex analyses
US9909171B2 (en) 2004-05-28 2018-03-06 Takara Bio Usa, Inc. Thermo-controllable high-density chips for multiplex analyses
US20060030037A1 (en) * 2004-05-28 2006-02-09 Victor Joseph Thermo-controllable high-density chips for multiplex analyses
US9132427B2 (en) 2007-01-22 2015-09-15 Wafergen, Inc. Apparatus for high throughput chemical reactions
US8252581B2 (en) 2007-01-22 2012-08-28 Wafergen, Inc. Apparatus for high throughput chemical reactions
US9951381B2 (en) 2007-01-22 2018-04-24 Takara Bio Usa, Inc. Apparatus for high throughput chemical reactions
US20080176290A1 (en) * 2007-01-22 2008-07-24 Victor Joseph Apparatus for high throughput chemical reactions
US11643681B2 (en) 2007-01-22 2023-05-09 Takara Bio Usa, Inc. Apparatus for high throughput chemical reactions
US10641772B2 (en) 2015-02-20 2020-05-05 Takara Bio Usa, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
US11125752B2 (en) 2015-02-20 2021-09-21 Takara Bio Usa, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
US10641703B2 (en) * 2016-01-07 2020-05-05 University Of Shanghai For Science And Technology Method of enhancing absorption spectral signals of biological samples in terahertz wavebands
US11460405B2 (en) 2016-07-21 2022-10-04 Takara Bio Usa, Inc. Multi-Z imaging and dispensing with multi-well devices

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US20100233698A1 (en) 2010-09-16
US20180135107A1 (en) 2018-05-17
US20060027317A1 (en) 2006-02-09
US20180135106A1 (en) 2018-05-17
US20060073491A1 (en) 2006-04-06
EP1758981A2 (fr) 2007-03-07
US7622296B2 (en) 2009-11-24
US7311794B2 (en) 2007-12-25
US9228933B2 (en) 2016-01-05
US9909171B2 (en) 2018-03-06
US10718014B2 (en) 2020-07-21
WO2005118773A3 (fr) 2006-02-02
US20060030035A1 (en) 2006-02-09
US20200299751A1 (en) 2020-09-24
US20060030037A1 (en) 2006-02-09
US20160138082A1 (en) 2016-05-19
EP1758981A4 (fr) 2013-01-16
US7833709B2 (en) 2010-11-16
US20160237477A1 (en) 2016-08-18
WO2005118773A2 (fr) 2005-12-15

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