GB2468993A - Thermal cycling instrument and methods for carrying out pcr - Google Patents

Thermal cycling instrument and methods for carrying out pcr Download PDF

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Publication number
GB2468993A
GB2468993A GB1012081A GB201012081A GB2468993A GB 2468993 A GB2468993 A GB 2468993A GB 1012081 A GB1012081 A GB 1012081A GB 201012081 A GB201012081 A GB 201012081A GB 2468993 A GB2468993 A GB 2468993A
Authority
GB
United Kingdom
Prior art keywords
samples
sample
carrying
methods
spaces
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB1012081A
Other versions
GB201012081D0 (en
Inventor
David A Cohen
Michael J Mortillaro
Charles Patrick Andro
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
FINNZYMES INSTR Oy
Thermo Fisher Scientific Oy
Original Assignee
FINNZYMES INSTR Oy
Finnzymes Instruments Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/965,660 external-priority patent/US20090165574A1/en
Priority claimed from FI20075960A external-priority patent/FI20075960A0/en
Application filed by FINNZYMES INSTR Oy, Finnzymes Instruments Oy filed Critical FINNZYMES INSTR Oy
Priority claimed from PCT/FI2008/050784 external-priority patent/WO2009101239A1/en
Publication of GB201012081D0 publication Critical patent/GB201012081D0/en
Publication of GB2468993A publication Critical patent/GB2468993A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/142—Preventing evaporation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates

Abstract

The invention relates to a polymerase chair reaction (PCR) process and a thermal cycler. In the process, biological samples are held in a sample carrier (10) having a plurality of sample spaces (12) each having upper and lower ends and the samples are sequentially heated and cooled. The thermal cycler according to the invention comprises heat transfer means for automatic heating and cooling of the samples in the sample carrier, heatable closure means (18) above the upper ends of the samples spaces (12) for preventing condensation of sample vapor during the process, and means for adjusting one process parameter, preferably the temperature of the heatable closure means, depending on at least one other of said process parameters. The invention helps to decrease the number of failed PCR experiments in particular due to changes in sample volume.
GB1012081A 2007-12-27 2008-12-22 Thermal cycling instrument and methods for carrying out pcr Withdrawn GB2468993A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/965,660 US20090165574A1 (en) 2007-12-27 2007-12-27 Instrument and method for nucleic acid amplification
FI20075960A FI20075960A0 (en) 2007-12-27 2007-12-27 New device and procedure
PCT/FI2008/050784 WO2009101239A1 (en) 2008-02-13 2008-12-22 System for pressurizing the inside space of a building, e.g. the stairway of an apartment block, in a fire scenario

Publications (2)

Publication Number Publication Date
GB201012081D0 GB201012081D0 (en) 2010-09-01
GB2468993A true GB2468993A (en) 2010-09-29

Family

ID=40642088

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1012081A Withdrawn GB2468993A (en) 2007-12-27 2008-12-22 Thermal cycling instrument and methods for carrying out pcr

Country Status (3)

Country Link
DE (1) DE112008003552T9 (en)
GB (1) GB2468993A (en)
WO (1) WO2009083648A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7311794B2 (en) 2004-05-28 2007-12-25 Wafergen, Inc. Methods of sealing micro wells
EP2109627A4 (en) 2007-01-22 2014-07-23 Wafergen Inc Apparatus for high throughput chemical reactions
WO2016134342A1 (en) 2015-02-20 2016-08-25 Wafergen, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
WO2018017892A1 (en) 2016-07-21 2018-01-25 Takara Bio Usa, Inc. Multi-z imaging and dispensing with multi-well devices
CN114367326A (en) * 2022-01-11 2022-04-19 无锡科智达科技有限公司 Temperature changing module with precise temperature compensation and compensation method

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100236506B1 (en) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 Apparatus for polymerase chain reaction
US20040166569A1 (en) 2001-07-13 2004-08-26 Andre Marziali Thermal cycling methods and apparatus
US6730883B2 (en) 2002-10-02 2004-05-04 Stratagene Flexible heating cover assembly for thermal cycling of samples of biological material

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BIO-RAD LABORATORIES: MJ mini Gradient Thermal Cycler Operations Manual [Online] 2005, pages 1-96 (www.bio-rad.com) *
FINNZYMES INSTRUMENT: Piko Thermal Cycler User Manual [Online] October 2007 pages 1-14 (www.finnzymes.com) *

Also Published As

Publication number Publication date
WO2009083648A3 (en) 2009-09-03
DE112008003552T5 (en) 2010-10-28
GB201012081D0 (en) 2010-09-01
WO2009083648A2 (en) 2009-07-09
DE112008003552T9 (en) 2011-03-10

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Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)