UA99263C2 - СПОСОБ ОЧИСТКИ Fc-СЛИТОГО БЕЛКА - Google Patents

СПОСОБ ОЧИСТКИ Fc-СЛИТОГО БЕЛКА

Info

Publication number
UA99263C2
UA99263C2 UAA200902919A UAA200902919A UA99263C2 UA 99263 C2 UA99263 C2 UA 99263C2 UA A200902919 A UAA200902919 A UA A200902919A UA A200902919 A UAA200902919 A UA A200902919A UA 99263 C2 UA99263 C2 UA 99263C2
Authority
UA
Ukraine
Prior art keywords
fusion protein
purification
chromatography
protein
exchange chromatography
Prior art date
Application number
UAA200902919A
Other languages
English (en)
Ukrainian (uk)
Inventor
Алекс Эон-Дюваль
Ален Лампруа
Original Assignee
Арес Трейдинг С.А.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Арес Трейдинг С.А. filed Critical Арес Трейдинг С.А.
Publication of UA99263C2 publication Critical patent/UA99263C2/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C07K1/28—Isoelectric focusing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Изобретение относится к способу очистки Fc-слитого белка, который имеет изоэлектрическую точку (pI) 6,9-9,5, который предусматривает подвергание жидкости, которая содержит указанный Fc-слитый белок, белок А- или белок G-аффинной хроматографии с последующей катионообменной хроматографией, анионообменной хроматографией и гидроксиапатитной хроматографией и сбор элюата для получения очищенного Fc-слитого белка.
UAA200902919A 2006-08-28 2007-08-27 СПОСОБ ОЧИСТКИ Fc-СЛИТОГО БЕЛКА UA99263C2 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP06119610 2006-08-28

Publications (1)

Publication Number Publication Date
UA99263C2 true UA99263C2 (ru) 2012-08-10

Family

ID=37038258

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA200902919A UA99263C2 (ru) 2006-08-28 2007-08-27 СПОСОБ ОЧИСТКИ Fc-СЛИТОГО БЕЛКА

Country Status (15)

Country Link
US (1) US20100267932A1 (ru)
EP (1) EP2061802A1 (ru)
KR (1) KR20090058532A (ru)
CN (1) CN101541825B (ru)
AR (1) AR062569A1 (ru)
AU (1) AU2007291282B2 (ru)
BR (1) BRPI0716139A2 (ru)
CA (1) CA2661872A1 (ru)
EA (1) EA017733B1 (ru)
IL (1) IL197221A (ru)
MX (1) MX2009002013A (ru)
NZ (1) NZ574623A (ru)
UA (1) UA99263C2 (ru)
WO (1) WO2008025747A1 (ru)
ZA (1) ZA200900836B (ru)

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Also Published As

Publication number Publication date
KR20090058532A (ko) 2009-06-09
IL197221A (en) 2013-06-27
WO2008025747A1 (en) 2008-03-06
CA2661872A1 (en) 2008-03-06
EA200970230A1 (ru) 2009-08-28
EP2061802A1 (en) 2009-05-27
IL197221A0 (en) 2009-12-24
MX2009002013A (es) 2009-03-09
AU2007291282A1 (en) 2008-03-06
EA017733B1 (ru) 2013-02-28
CN101541825B (zh) 2013-08-14
US20100267932A1 (en) 2010-10-21
BRPI0716139A2 (pt) 2013-09-17
AU2007291282B2 (en) 2011-10-20
ZA200900836B (en) 2010-05-26
CN101541825A (zh) 2009-09-23
NZ574623A (en) 2011-12-22
AR062569A1 (es) 2008-11-19

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