UA98681C2 - Способ получения фибробластоподобных клеток из пуповинного канатика новорожденного - Google Patents
Способ получения фибробластоподобных клеток из пуповинного канатика новорожденного Download PDFInfo
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- UA98681C2 UA98681C2 UAA201007593A UAA201007593A UA98681C2 UA 98681 C2 UA98681 C2 UA 98681C2 UA A201007593 A UAA201007593 A UA A201007593A UA A201007593 A UAA201007593 A UA A201007593A UA 98681 C2 UA98681 C2 UA 98681C2
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- cells
- umbilical cord
- fibroblast
- solution
- vein
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Gynecology & Obstetrics (AREA)
- Biotechnology (AREA)
- Reproductive Health (AREA)
- Developmental Biology & Embryology (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Pregnancy & Childbirth (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
Abstract
Изобретение относится к способу получения фибробластоподобных клеток из пуповинного канатика новорожденного, что включает гомогенизацию пуповинного канатика и обработку гомогената раствором, содержащим коллагеназу типа I и коллагеназу типа IV при соотношении ферментов 1:1 в пропорции гомогената к раствору ферментов 1:5.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008111708/13A RU2384618C2 (ru) | 2008-03-27 | 2008-03-27 | Способ получения фибробластоподобных клеток из пупочного канатика новорожденного |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA98681C2 true UA98681C2 (ru) | 2012-06-11 |
Family
ID=41114168
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UAA201007593A UA98681C2 (ru) | 2008-03-27 | 2009-03-17 | Способ получения фибробластоподобных клеток из пуповинного канатика новорожденного |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP2277994B1 (ru) |
| CN (1) | CN101978048A (ru) |
| EA (1) | EA017967B1 (ru) |
| RU (1) | RU2384618C2 (ru) |
| UA (1) | UA98681C2 (ru) |
| WO (1) | WO2009120111A1 (ru) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2519326C2 (ru) | 2011-12-29 | 2014-06-10 | Общество с ограниченной ответственностью "НекстГен" | Биокомпозит для обеспечения восстановительных процессов после повреждения у млекопитающего, способ его получения (варианты) и применения |
| CN103478118B (zh) * | 2013-10-09 | 2014-11-26 | 山东省农业科学院畜牧兽医研究所 | 一种用于细胞培养的组织块的冷冻保存方法 |
| RU2710263C1 (ru) * | 2019-09-19 | 2019-12-25 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Самарский государственный медицинский университет" Министерства здравоохранения Российской Федерации | Способ получения и культивирования фибробластоподобных клеток из пуповины новорожденного |
| RU2744301C1 (ru) * | 2020-07-21 | 2021-03-05 | федеральное государственное автономное образовательное учреждение высшего образования Первый Московский государственный медицинский университет имени И.М. Сеченова Министерства здравоохранения Российской Федерации (Сеченовский университет) (ФГАОУ ВО Первый МГМУ им. И.М. Сеченова Минздрава России (Се | Способ получения мультипотентных мезенхимных стромальных клеток из пупочного канатика новорожденного |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002092794A2 (en) * | 2001-05-17 | 2002-11-21 | Isotis N.V. | Isolation method of mesenchymal cells |
| KR101322889B1 (ko) * | 2004-08-16 | 2013-10-30 | 셀리서치 코포레이션 피티이 리미티드 | 제대의 양막으로부터 줄기/전구세포의 추출 |
| RU2308957C1 (ru) * | 2006-03-24 | 2007-10-27 | Общество с ограниченной ответственностью "Медицинские технологии" | Способ получения препарата для мезотерапии и препарат, полученный этим способом (варианты) |
| US7993918B2 (en) * | 2006-08-04 | 2011-08-09 | Anthrogenesis Corporation | Tumor suppression using placental stem cells |
-
2008
- 2008-03-27 RU RU2008111708/13A patent/RU2384618C2/ru active IP Right Revival
-
2009
- 2009-03-17 WO PCT/RU2009/000128 patent/WO2009120111A1/ru active Application Filing
- 2009-03-17 CN CN2009801106759A patent/CN101978048A/zh active Pending
- 2009-03-17 UA UAA201007593A patent/UA98681C2/ru unknown
- 2009-03-17 EP EP09725611.9A patent/EP2277994B1/en not_active Not-in-force
- 2009-03-17 EA EA201000377A patent/EA017967B1/ru active IP Right Revival
Also Published As
| Publication number | Publication date |
|---|---|
| EA017967B1 (ru) | 2013-04-30 |
| CN101978048A (zh) | 2011-02-16 |
| RU2008111708A (ru) | 2009-10-10 |
| RU2384618C2 (ru) | 2010-03-20 |
| EP2277994A4 (en) | 2013-06-05 |
| EP2277994A1 (en) | 2011-01-26 |
| EP2277994B1 (en) | 2018-02-21 |
| WO2009120111A1 (ru) | 2009-10-01 |
| EA201000377A1 (ru) | 2010-06-30 |
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