UA108071C2 - Спосіб поліпшення витривалості до нестачі азоту у рослини - Google Patents

Спосіб поліпшення витривалості до нестачі азоту у рослини

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Publication number
UA108071C2
UA108071C2 UAA201113238A UAA201113238A UA108071C2 UA 108071 C2 UA108071 C2 UA 108071C2 UA A201113238 A UAA201113238 A UA A201113238A UA A201113238 A UAA201113238 A UA A201113238A UA 108071 C2 UA108071 C2 UA 108071C2
Authority
UA
Ukraine
Prior art keywords
specified
plant
nucleic acid
heterologous polynucleotide
endurance
Prior art date
Application number
UAA201113238A
Other languages
English (en)
Russian (ru)
Inventor
Ніколас Дж. Байт
Сара Т. Коллінсон
Джефрі І. Хеббен
Хонор Рені Лефіт
Келі Рейменн
Original Assignee
Піонер Хай-Бред Інтернешнл, Інк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Піонер Хай-Бред Інтернешнл, Інк. filed Critical Піонер Хай-Бред Інтернешнл, Інк.
Publication of UA108071C2 publication Critical patent/UA108071C2/uk

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance

Abstract

Даний винахід забезпечує спосіб поліпшення витривалості до нестачі азоту у рослин. Спосіб включає інгібування синтезу етилену в рослині шляхом стійкого трансформування рослини гетерологічним полінуклеотидом, що знижує експресію ACC-синтази при експресії зазначеного гетерологічного полінуклеотиду, та вирощування зазначеної рослини в умовах обмеження азоту, за допомогою чого експресується зазначений гетерологічний полінуклеотид, і зазначена рослина демонструє поліпшену витривалість до нестачі азоту в порівнянні з контрольною рослиною. Зазначений гетерологічний полінуклеотид включає нуклеїнову кислоту, що кодує транскрипт, який здатний утворювати дволанцюгову РНК і опосередковувати РНК-інтерференцію нуклеїнової кислоти ACC-синтази. Зазначена нуклеїнова кислота включає першу нуклеотидну послідовність, яка включає щонайменше 21 суміжний нуклеотид нуклеїнової кислоти ACC-синтази і другу нуклеотидну послідовність, що включає комплементарну послідовність зазначеної першої нуклеотидної послідовності, де перший і другий нуклеотиди відокремлені нітроном.
UAA201113238A 2009-04-14 2010-04-14 Спосіб поліпшення витривалості до нестачі азоту у рослини UA108071C2 (uk)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US16908209P 2009-04-14 2009-04-14
US24806009P 2009-10-02 2009-10-02
US29090209P 2009-12-30 2009-12-30
PCT/US2010/031008 WO2010120862A1 (en) 2009-04-14 2010-04-14 Modulation of acc synthase improves plant yield under low nitrogen conditions

Publications (1)

Publication Number Publication Date
UA108071C2 true UA108071C2 (uk) 2015-03-25

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
UAA201113238A UA108071C2 (uk) 2009-04-14 2010-04-14 Спосіб поліпшення витривалості до нестачі азоту у рослини

Country Status (10)

Country Link
US (2) US8987553B2 (uk)
EP (1) EP2419510B1 (uk)
CN (1) CN102395674B (uk)
BR (1) BRPI1014658A2 (uk)
CA (1) CA2757581A1 (uk)
EA (1) EA201171245A1 (uk)
MX (1) MX2011010845A (uk)
UA (1) UA108071C2 (uk)
WO (1) WO2010120862A1 (uk)
ZA (1) ZA201106920B (uk)

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CA2757581A1 (en) 2010-10-21
BRPI1014658A2 (pt) 2019-09-24
EP2419510B1 (en) 2015-09-16
US20150284737A1 (en) 2015-10-08
WO2010120862A1 (en) 2010-10-21
ZA201106920B (en) 2012-05-30
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EP2419510A1 (en) 2012-02-22
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