UA108071C2 - Способ улучшения выносливости к недостатку азота у растения - Google Patents

Способ улучшения выносливости к недостатку азота у растения

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Publication number
UA108071C2
UA108071C2 UAA201113238A UAA201113238A UA108071C2 UA 108071 C2 UA108071 C2 UA 108071C2 UA A201113238 A UAA201113238 A UA A201113238A UA A201113238 A UAA201113238 A UA A201113238A UA 108071 C2 UA108071 C2 UA 108071C2
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UA
Ukraine
Prior art keywords
specified
plant
nucleic acid
heterologous polynucleotide
endurance
Prior art date
Application number
UAA201113238A
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English (en)
Ukrainian (uk)
Inventor
Ніколас Дж. Байт
Сара Т. Коллінсон
Джефрі І. Хеббен
Хонор Рені Лефіт
Келі Рейменн
Original Assignee
Піонер Хай-Бред Інтернешнл, Інк.
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Application filed by Піонер Хай-Бред Інтернешнл, Інк. filed Critical Піонер Хай-Бред Інтернешнл, Інк.
Publication of UA108071C2 publication Critical patent/UA108071C2/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Изобретение обеспечивает способ улучшения выносливости к недостатку азота у растений. Способ включает ингибирование синтеза этилена в растении путем устойчивой трансформации растения гетерологическим полинуклеотидом, что снижает экспрессию ACC-синтазы при экспрессии указанного гетерологического полинуклеотида, и выращивание указанного растения в условиях ограничения азота, с помощью чего экспрессируется указанный гетерологический полинуклеотид, и указанное растение демонстрирует улучшенную устойчивость к недостатку азота по сравнению с контрольным растением. Указанный гетерологический полинуклеотид включает нуклеиновую кислоту, кодирующую транскрипт, который способен образовывать двухцепочечную РНК и опосредовать РНК-интерференцию нуклеиновой кислоты ACC-синтазы. Указанная нуклеиновая кислота включает первую нуклеотидную последовательность, которая включает как минимум 21 смежный нуклеотид нуклеиновой кислоты ACC-синтазы, и вторую нуклеотидную последовательность, включая комплементарную последовательность указанной первой нуклеотидной последовательности, где первый и второй нуклеотиды отделены нитроном.
UAA201113238A 2009-04-14 2010-04-14 Способ улучшения выносливости к недостатку азота у растения UA108071C2 (ru)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US16908209P 2009-04-14 2009-04-14
US24806009P 2009-10-02 2009-10-02
US29090209P 2009-12-30 2009-12-30
PCT/US2010/031008 WO2010120862A1 (en) 2009-04-14 2010-04-14 Modulation of acc synthase improves plant yield under low nitrogen conditions

Publications (1)

Publication Number Publication Date
UA108071C2 true UA108071C2 (ru) 2015-03-25

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Application Number Title Priority Date Filing Date
UAA201113238A UA108071C2 (ru) 2009-04-14 2010-04-14 Способ улучшения выносливости к недостатку азота у растения

Country Status (10)

Country Link
US (2) US8987553B2 (ru)
EP (1) EP2419510B1 (ru)
CN (1) CN102395674B (ru)
BR (1) BRPI1014658A2 (ru)
CA (1) CA2757581A1 (ru)
EA (1) EA201171245A1 (ru)
MX (1) MX2011010845A (ru)
UA (1) UA108071C2 (ru)
WO (1) WO2010120862A1 (ru)
ZA (1) ZA201106920B (ru)

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BRPI1014658A2 (pt) 2019-09-24
US20150284737A1 (en) 2015-10-08
EA201171245A1 (ru) 2012-05-30
US20100287669A1 (en) 2010-11-11
MX2011010845A (es) 2011-11-02
CN102395674A (zh) 2012-03-28
WO2010120862A1 (en) 2010-10-21
EP2419510B1 (en) 2015-09-16
US8987553B2 (en) 2015-03-24
CA2757581A1 (en) 2010-10-21
EP2419510A1 (en) 2012-02-22
ZA201106920B (en) 2012-05-30

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