TWI890888B - ＦｏｋＩ核酸酶結構域之突變體 - Google Patents

ＦｏｋＩ核酸酶結構域之突變體

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Publication number
TWI890888B
TWI890888B TW110141338A TW110141338A TWI890888B TW I890888 B TWI890888 B TW I890888B TW 110141338 A TW110141338 A TW 110141338A TW 110141338 A TW110141338 A TW 110141338A TW I890888 B TWI890888 B TW I890888B
Authority
TW
Taiwan
Prior art keywords
amino acid
foki
protein
substitution
nucleic acid
Prior art date
Application number
TW110141338A
Other languages
English (en)
Chinese (zh)
Other versions
TW202233695A (zh
Inventor
八木祐介
太田賢
中村崇裕
Original Assignee
日商基因編輯力股份有限公司
國立大學法人九州大學
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 日商基因編輯力股份有限公司, 國立大學法人九州大學 filed Critical 日商基因編輯力股份有限公司
Publication of TW202233695A publication Critical patent/TW202233695A/zh
Application granted granted Critical
Publication of TWI890888B publication Critical patent/TWI890888B/zh

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
TW110141338A 2020-11-06 2021-11-05 ＦｏｋＩ核酸酶結構域之突變體 TWI890888B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2020185619 2020-11-06
JP2020-185619 2020-11-06

Publications (2)

Publication Number Publication Date
TW202233695A TW202233695A (zh) 2022-09-01
TWI890888B true TWI890888B (zh) 2025-07-21

Family

ID=81457062

Family Applications (1)

Application Number Title Priority Date Filing Date
TW110141338A TWI890888B (zh) 2020-11-06 2021-11-05 ＦｏｋＩ核酸酶結構域之突變體

Country Status (6)

Country Link
US (1) US12637667B2 (cg-RX-API-DMAC7.html)
EP (1) EP4242237A4 (cg-RX-API-DMAC7.html)
JP (1) JP7752342B2 (cg-RX-API-DMAC7.html)
CN (1) CN116406383A (cg-RX-API-DMAC7.html)
TW (1) TWI890888B (cg-RX-API-DMAC7.html)
WO (1) WO2022097663A1 (cg-RX-API-DMAC7.html)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7125727B1 (ja) 2021-09-07 2022-08-25 国立大学法人千葉大学 核酸配列改変用組成物および核酸配列の標的部位を改変する方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011097036A1 (en) * 2010-02-08 2011-08-11 Sangamo Biosciences, Inc. Engineered cleavage half-domains

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ATE347593T1 (de) 2002-01-23 2006-12-15 Univ Utah Res Found Zielgerichtete chromosomale mutagenese mit zinkfingernukleasen
US20110239315A1 (en) 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
PT2816112T (pt) 2009-12-10 2018-11-20 Univ Iowa State Res Found Inc Modificação do adn modificada pelo efector tal
EP2526199A4 (en) 2010-01-22 2013-08-07 Scripps Research Inst PROCESS FOR PRODUCING ZINC FINGERUCLEASES WITH CHANGED EFFECT
WO2012093833A2 (en) 2011-01-03 2012-07-12 Toolgen Incorporation Genome engineering via designed tal effector nucleases
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
ES2728782T3 (es) 2012-05-25 2019-10-28 Univ California Métodos y composiciones para la modificación de ADN objetivo dirigida por ARN y para la modulación de la transcripción dirigida por ARN
MX380562B (es) 2012-12-12 2025-03-12 Broad Inst Inc Modificaciones de sistemas, métodos y composiciones guía optimizadas para la manipulación de secuencias.
KR20160007541A (ko) 2013-04-22 2016-01-20 고쿠리쓰다이가쿠호진 규슈다이가쿠 피피알 모티프를 이용한 디앤에이 결합성 단백질 및 그의 이용
JP5931022B2 (ja) 2013-08-09 2016-06-08 国立大学法人広島大学 Ｄｎａ結合ドメインを含むポリペプチド
AU2015292744C1 (en) * 2014-07-21 2021-01-21 Novartis Ag Treatment of cancer using humanized anti-BCMA chimeric antigen receptor
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
IL264792B2 (en) 2016-08-24 2023-10-01 Sangamo Therapeutics Inc Engineered target-specific zinc-finger nucleases
US11371030B2 (en) 2017-05-31 2022-06-28 The University Of Tokyo Modified Cas9 protein and use thereof
KR102541398B1 (ko) 2017-06-08 2023-06-07 고꾸리쯔 다이가꾸 호우징 오사까 다이가꾸 Dna가 편집된 진핵 세포를 제조하는 방법 및 당해 방법에 사용되는 키트

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2011097036A1 (en) * 2010-02-08 2011-08-11 Sangamo Biosciences, Inc. Engineered cleavage half-domains

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
期刊 Guo, Jing et al. Directed evolution of an enhanced and highly efficient FokI cleavage domain for zinc finger nucleases. Journal of molecular biology vol. 400 Elsevier 2010 96-107
期刊 Guo, Jing et al. Directed evolution of an enhanced and highly efficient FokI cleavage domain for zinc finger nucleases. Journal of molecular biology vol. 400 Elsevier 2010 96-107;期刊 Miller, Jeffrey C et al. Enhancing gene editing specificity by attenuating DNA cleavage kinetics. Nature biotechnology vol. 37 Nature Portfolio 2019 945-952 *
期刊 Miller, Jeffrey C et al. Enhancing gene editing specificity by attenuating DNA cleavage kinetics. Nature biotechnology vol. 37 Nature Portfolio 2019 945-952

Also Published As

Publication number Publication date
EP4242237A1 (en) 2023-09-13
TW202233695A (zh) 2022-09-01
CN116406383A (zh) 2023-07-07
EP4242237A4 (en) 2025-01-01
JPWO2022097663A1 (cg-RX-API-DMAC7.html) 2022-05-12
JP7752342B2 (ja) 2025-10-10
WO2022097663A1 (ja) 2022-05-12
US20230416709A1 (en) 2023-12-28
US12637667B2 (en) 2026-05-26

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