JP7752342B2 - ＦｏｋＩヌクレアーゼドメインの変異体 - Google Patents

ＦｏｋＩヌクレアーゼドメインの変異体

Info

Publication number
JP7752342B2
JP7752342B2 JP2022560800A JP2022560800A JP7752342B2 JP 7752342 B2 JP7752342 B2 JP 7752342B2 JP 2022560800 A JP2022560800 A JP 2022560800A JP 2022560800 A JP2022560800 A JP 2022560800A JP 7752342 B2 JP7752342 B2 JP 7752342B2
Authority
JP
Japan
Prior art keywords
substitution
amino acid
foki
protein
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022560800A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2022097663A1 (cg-RX-API-DMAC7.html
Inventor
祐介 八木
賢 太田
崇裕 中村
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EDITFORCE, INC.
Kyushu University
Original Assignee
EDITFORCE, INC.
Kyushu University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EDITFORCE, INC., Kyushu University filed Critical EDITFORCE, INC.
Publication of JPWO2022097663A1 publication Critical patent/JPWO2022097663A1/ja
Application granted granted Critical
Publication of JP7752342B2 publication Critical patent/JP7752342B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2022560800A 2020-11-06 2021-11-04 ＦｏｋＩヌクレアーゼドメインの変異体 Active JP7752342B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020185619 2020-11-06
JP2020185619 2020-11-06
PCT/JP2021/040503 WO2022097663A1 (ja) 2020-11-06 2021-11-04 ＦｏｋＩヌクレアーゼドメインの変異体

Publications (2)

Publication Number Publication Date
JPWO2022097663A1 JPWO2022097663A1 (cg-RX-API-DMAC7.html) 2022-05-12
JP7752342B2 true JP7752342B2 (ja) 2025-10-10

Family

ID=81457062

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022560800A Active JP7752342B2 (ja) 2020-11-06 2021-11-04 ＦｏｋＩヌクレアーゼドメインの変異体

Country Status (6)

Country Link
US (1) US12637667B2 (cg-RX-API-DMAC7.html)
EP (1) EP4242237A4 (cg-RX-API-DMAC7.html)
JP (1) JP7752342B2 (cg-RX-API-DMAC7.html)
CN (1) CN116406383A (cg-RX-API-DMAC7.html)
TW (1) TWI890888B (cg-RX-API-DMAC7.html)
WO (1) WO2022097663A1 (cg-RX-API-DMAC7.html)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7125727B1 (ja) 2021-09-07 2022-08-25 国立大学法人千葉大学 核酸配列改変用組成物および核酸配列の標的部位を改変する方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011091324A2 (en) 2010-01-22 2011-07-28 The Scripps Research Institute Methods of generating zinc finger nucleases having altered activity
WO2011097036A1 (en) 2010-02-08 2011-08-11 Sangamo Biosciences, Inc. Engineered cleavage half-domains
US20130217131A1 (en) 2011-01-03 2013-08-22 Toolgen Incorporation Genome engineering via designed tal effector nucleases
WO2018039448A1 (en) 2016-08-24 2018-03-01 Sangamo Therapeutics, Inc. Engineered target specific nucleases

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE347593T1 (de) 2002-01-23 2006-12-15 Univ Utah Res Found Zielgerichtete chromosomale mutagenese mit zinkfingernukleasen
US20110239315A1 (en) 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
PT2816112T (pt) 2009-12-10 2018-11-20 Univ Iowa State Res Found Inc Modificação do adn modificada pelo efector tal
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
ES2728782T3 (es) 2012-05-25 2019-10-28 Univ California Métodos y composiciones para la modificación de ADN objetivo dirigida por ARN y para la modulación de la transcripción dirigida por ARN
MX380562B (es) 2012-12-12 2025-03-12 Broad Inst Inc Modificaciones de sistemas, métodos y composiciones guía optimizadas para la manipulación de secuencias.
KR20160007541A (ko) 2013-04-22 2016-01-20 고쿠리쓰다이가쿠호진 규슈다이가쿠 피피알 모티프를 이용한 디앤에이 결합성 단백질 및 그의 이용
JP5931022B2 (ja) 2013-08-09 2016-06-08 国立大学法人広島大学 Ｄｎａ結合ドメインを含むポリペプチド
AU2015292744C1 (en) * 2014-07-21 2021-01-21 Novartis Ag Treatment of cancer using humanized anti-BCMA chimeric antigen receptor
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
US11371030B2 (en) 2017-05-31 2022-06-28 The University Of Tokyo Modified Cas9 protein and use thereof
KR102541398B1 (ko) 2017-06-08 2023-06-07 고꾸리쯔 다이가꾸 호우징 오사까 다이가꾸 Dna가 편집된 진핵 세포를 제조하는 방법 및 당해 방법에 사용되는 키트

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011091324A2 (en) 2010-01-22 2011-07-28 The Scripps Research Institute Methods of generating zinc finger nucleases having altered activity
WO2011097036A1 (en) 2010-02-08 2011-08-11 Sangamo Biosciences, Inc. Engineered cleavage half-domains
US20130217131A1 (en) 2011-01-03 2013-08-22 Toolgen Incorporation Genome engineering via designed tal effector nucleases
WO2018039448A1 (en) 2016-08-24 2018-03-01 Sangamo Therapeutics, Inc. Engineered target specific nucleases

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GUO J. et al.，Directed evolution of an enhanced and highly efficient fokI cleavage domain for zinc finger nuclease，J. Mol. Biol.，2010年，400，pp.96-107, Supplementary Information
MILLER J. C. et al.，Enhancing gene editing specificity by attenuating DNA cleavage kinetics，NATURE BIOTECHNOLOGY，2019年，Vol.37，pp.945-952, Supplementary Tables
WAUGH D. S. et al.，A novel class of FokI restriction endonuclease mutants that cleave hemi-methylated substrates，J. Biol. Chem.，1994年，Vol.269 No.16，pp.12298-12303

Also Published As

Publication number Publication date
EP4242237A1 (en) 2023-09-13
TWI890888B (zh) 2025-07-21
TW202233695A (zh) 2022-09-01
CN116406383A (zh) 2023-07-07
EP4242237A4 (en) 2025-01-01
JPWO2022097663A1 (cg-RX-API-DMAC7.html) 2022-05-12
WO2022097663A1 (ja) 2022-05-12
US20230416709A1 (en) 2023-12-28
US12637667B2 (en) 2026-05-26

Similar Documents

Publication Publication Date Title
US12371713B2 (en) Method for producing DNA-edited eukaryotic cell, and kit used in the same
CN106795521B (zh) 用于修饰所靶向基因座的方法和组合物
KR102468108B1 (ko) 표적화된 유전자 변형을 위한 방법 및 그 조성물, 및 사용 방법
US20160298125A1 (en) Crispr-based genome modification and regulation
CN107058383A (zh) 经遗传修饰的动物及其生成方法
CN110199020A (zh) 用于快速产生用于细胞系开发的同源重组载体的dna质粒
KR102854193B1 (ko) 녹인 세포의 제작 방법
TWI890888B (zh) ＦｏｋＩ核酸酶結構域之突變體
WO2022050377A1 (ja) 標的ｄｎａの編集方法、標的ｄｎａが編集された細胞の製造方法、及びそれらに用いるｄｎａ編集システム
KR20250161567A (ko) 닉킹 효소, dna 편집 시스템, 표적 dna의 편집 방법 및 표적 dna가 편집된 세포의 제조 방법
WO2025197847A1 (ja) 改良されたＣＲＩＳＰＲ－Ｃａｓ３システム
JP2023131616A (ja) Ｄｎａ編集システム、並びに、それを用いた標的ｄｎａの編集方法及び標的ｄｎａが編集された細胞の製造方法
WO2025206046A1 (ja) 所望のヌクレオチド配列をノックインする方法、ノックイン細胞の製造方法、及びキット
CN120187848A (zh) 双链切割dna的核酸酶结构域
HK40021189A (en) Method for manufacturing dna-edited eukaryotic cell, and kit used in method
HK40021189B (en) Method for manufacturing dna-edited eukaryotic cell, and kit used in method

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20240529

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20250526

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250710

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20250911

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20250917

R150 Certificate of patent or registration of utility model

Ref document number: 7752342

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150