CN116406383A - FokI核酸酶结构域的突变体 - Google Patents

FokI核酸酶结构域的突变体 Download PDF

Info

Publication number
CN116406383A
CN116406383A CN202180074640.5A CN202180074640A CN116406383A CN 116406383 A CN116406383 A CN 116406383A CN 202180074640 A CN202180074640 A CN 202180074640A CN 116406383 A CN116406383 A CN 116406383A
Authority
CN
China
Prior art keywords
protein
amino acid
foki
substitution
corresponding position
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180074640.5A
Other languages
English (en)
Chinese (zh)
Inventor
八木祐介
太田贤
中村崇裕
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japanese Gene Editing Co ltd
Kyushu University
Original Assignee
Japanese Gene Editing Co ltd
Kyushu University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japanese Gene Editing Co ltd, Kyushu University filed Critical Japanese Gene Editing Co ltd
Publication of CN116406383A publication Critical patent/CN116406383A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202180074640.5A 2020-11-06 2021-11-04 FokI核酸酶结构域的突变体 Pending CN116406383A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020185619 2020-11-06
JP2020-185619 2020-11-06
PCT/JP2021/040503 WO2022097663A1 (ja) 2020-11-06 2021-11-04 ＦｏｋＩヌクレアーゼドメインの変異体

Publications (1)

Publication Number Publication Date
CN116406383A true CN116406383A (zh) 2023-07-07

Family

ID=81457062

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180074640.5A Pending CN116406383A (zh) 2020-11-06 2021-11-04 FokI核酸酶结构域的突变体

Country Status (6)

Country Link
US (1) US12637667B2 (cg-RX-API-DMAC7.html)
EP (1) EP4242237A4 (cg-RX-API-DMAC7.html)
JP (1) JP7752342B2 (cg-RX-API-DMAC7.html)
CN (1) CN116406383A (cg-RX-API-DMAC7.html)
TW (1) TWI890888B (cg-RX-API-DMAC7.html)
WO (1) WO2022097663A1 (cg-RX-API-DMAC7.html)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7125727B1 (ja) 2021-09-07 2022-08-25 国立大学法人千葉大学 核酸配列改変用組成物および核酸配列の標的部位を改変する方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE347593T1 (de) 2002-01-23 2006-12-15 Univ Utah Res Found Zielgerichtete chromosomale mutagenese mit zinkfingernukleasen
US20110239315A1 (en) 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
PT2816112T (pt) 2009-12-10 2018-11-20 Univ Iowa State Res Found Inc Modificação do adn modificada pelo efector tal
EP2526199A4 (en) 2010-01-22 2013-08-07 Scripps Research Inst PROCESS FOR PRODUCING ZINC FINGERUCLEASES WITH CHANGED EFFECT
JP6137596B2 (ja) 2010-02-08 2017-05-31 サンガモ セラピューティクス， インコーポレイテッド 遺伝子操作された切断ハーフドメイン
WO2012093833A2 (en) 2011-01-03 2012-07-12 Toolgen Incorporation Genome engineering via designed tal effector nucleases
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
ES2728782T3 (es) 2012-05-25 2019-10-28 Univ California Métodos y composiciones para la modificación de ADN objetivo dirigida por ARN y para la modulación de la transcripción dirigida por ARN
MX380562B (es) 2012-12-12 2025-03-12 Broad Inst Inc Modificaciones de sistemas, métodos y composiciones guía optimizadas para la manipulación de secuencias.
KR20160007541A (ko) 2013-04-22 2016-01-20 고쿠리쓰다이가쿠호진 규슈다이가쿠 피피알 모티프를 이용한 디앤에이 결합성 단백질 및 그의 이용
JP5931022B2 (ja) 2013-08-09 2016-06-08 国立大学法人広島大学 Ｄｎａ結合ドメインを含むポリペプチド
AU2015292744C1 (en) * 2014-07-21 2021-01-21 Novartis Ag Treatment of cancer using humanized anti-BCMA chimeric antigen receptor
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
IL264792B2 (en) 2016-08-24 2023-10-01 Sangamo Therapeutics Inc Engineered target-specific zinc-finger nucleases
US11371030B2 (en) 2017-05-31 2022-06-28 The University Of Tokyo Modified Cas9 protein and use thereof
KR102541398B1 (ko) 2017-06-08 2023-06-07 고꾸리쯔 다이가꾸 호우징 오사까 다이가꾸 Dna가 편집된 진핵 세포를 제조하는 방법 및 당해 방법에 사용되는 키트

Also Published As

Publication number Publication date
EP4242237A1 (en) 2023-09-13
TWI890888B (zh) 2025-07-21
TW202233695A (zh) 2022-09-01
EP4242237A4 (en) 2025-01-01
JPWO2022097663A1 (cg-RX-API-DMAC7.html) 2022-05-12
JP7752342B2 (ja) 2025-10-10
WO2022097663A1 (ja) 2022-05-12
US20230416709A1 (en) 2023-12-28
US12637667B2 (en) 2026-05-26

Similar Documents

Publication Publication Date Title
US12371713B2 (en) Method for producing DNA-edited eukaryotic cell, and kit used in the same
EP2863736B1 (en) Genetically edited animals and methods for making the same
CN107090470B (zh) 经遗传修饰的动物及其生成方法
US20200029538A1 (en) Genome editing method
TR201816074T4 (tr) Hedeflenen genetik modifikasyonlara yönelik yöntemler ve bileşimler ve kullanım yöntemleri.
US10893667B2 (en) Non-meiotic allele introgression
CN108347908A (zh) 具有增加的热耐受性的经遗传修饰的动物
JP2019523009A (ja) Ｃ末端切断型フィブリリン−１の発現をもたらす変異を有するマウス
KR102854193B1 (ko) 녹인 세포의 제작 방법
TWI890888B (zh) ＦｏｋＩ核酸酶結構域之突變體
KR20250161567A (ko) 닉킹 효소, dna 편집 시스템, 표적 dna의 편집 방법 및 표적 dna가 편집된 세포의 제조 방법
WO2025197847A1 (ja) 改良されたＣＲＩＳＰＲ－Ｃａｓ３システム
WO2025206046A1 (ja) 所望のヌクレオチド配列をノックインする方法、ノックイン細胞の製造方法、及びキット
JP2023131616A (ja) Ｄｎａ編集システム、並びに、それを用いた標的ｄｎａの編集方法及び標的ｄｎａが編集された細胞の製造方法
WO2024116373A1 (ja) Ｄｎａを二本鎖切断するヌクレアーゼドメイン

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination