TWI784045B - 玻尿酸產生促進用組成物 - Google Patents
玻尿酸產生促進用組成物 Download PDFInfo
- Publication number
- TWI784045B TWI784045B TW107130593A TW107130593A TWI784045B TW I784045 B TWI784045 B TW I784045B TW 107130593 A TW107130593 A TW 107130593A TW 107130593 A TW107130593 A TW 107130593A TW I784045 B TWI784045 B TW I784045B
- Authority
- TW
- Taiwan
- Prior art keywords
- composition
- lactobacillus plantarum
- hyaluronic acid
- item
- immune cells
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 62
- 230000001737 promoting effect Effects 0.000 title claims abstract description 34
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 title claims description 37
- 229920002674 hyaluronan Polymers 0.000 title claims description 37
- 229960003160 hyaluronic acid Drugs 0.000 title claims description 37
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 66
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 66
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 66
- 210000002950 fibroblast Anatomy 0.000 claims abstract description 36
- 230000014509 gene expression Effects 0.000 claims abstract description 36
- 230000035755 proliferation Effects 0.000 claims abstract description 18
- 235000013305 food Nutrition 0.000 claims description 33
- 238000004519 manufacturing process Methods 0.000 claims description 31
- 210000002865 immune cell Anatomy 0.000 claims description 29
- 101710128038 Hyaluronan synthase Proteins 0.000 claims description 20
- 239000003814 drug Substances 0.000 claims description 15
- 206010003246 arthritis Diseases 0.000 claims description 13
- 208000017520 skin disease Diseases 0.000 claims description 13
- 235000013373 food additive Nutrition 0.000 claims description 10
- 239000002778 food additive Substances 0.000 claims description 10
- 235000015872 dietary supplement Nutrition 0.000 claims description 9
- 239000013589 supplement Substances 0.000 claims description 9
- 241000124008 Mammalia Species 0.000 claims description 6
- 230000006872 improvement Effects 0.000 claims description 6
- 230000002265 prevention Effects 0.000 claims description 2
- 108090000320 Hyaluronan Synthases Proteins 0.000 abstract 2
- 230000000694 effects Effects 0.000 description 36
- 241000894006 Bacteria Species 0.000 description 17
- 230000000052 comparative effect Effects 0.000 description 15
- 238000000034 method Methods 0.000 description 15
- 239000002609 medium Substances 0.000 description 14
- 239000000463 material Substances 0.000 description 12
- 239000002537 cosmetic Substances 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 9
- 101710197057 Hyaluronan synthase 1 Proteins 0.000 description 8
- 102100040203 Hyaluronan synthase 1 Human genes 0.000 description 8
- 229940079593 drug Drugs 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 101710197056 Hyaluronan synthase 2 Proteins 0.000 description 7
- 102100040206 Hyaluronan synthase 2 Human genes 0.000 description 7
- -1 for example Substances 0.000 description 7
- 239000013642 negative control Substances 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 235000013361 beverage Nutrition 0.000 description 6
- 210000000845 cartilage Anatomy 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 5
- 206010007710 Cartilage injury Diseases 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 5
- 229920002472 Starch Polymers 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 238000011156 evaluation Methods 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- 210000004989 spleen cell Anatomy 0.000 description 5
- 239000008107 starch Substances 0.000 description 5
- 235000019698 starch Nutrition 0.000 description 5
- 208000006820 Arthralgia Diseases 0.000 description 4
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- 206010012438 Dermatitis atopic Diseases 0.000 description 4
- 208000012659 Joint disease Diseases 0.000 description 4
- 201000004681 Psoriasis Diseases 0.000 description 4
- 238000000692 Student's t-test Methods 0.000 description 4
- 201000008937 atopic dermatitis Diseases 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 4
- 239000012228 culture supernatant Substances 0.000 description 4
- 230000007547 defect Effects 0.000 description 4
- 238000003306 harvesting Methods 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 210000000629 knee joint Anatomy 0.000 description 4
- 208000024765 knee pain Diseases 0.000 description 4
- 230000005499 meniscus Effects 0.000 description 4
- 235000012149 noodles Nutrition 0.000 description 4
- 230000001225 therapeutic effect Effects 0.000 description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 102000009024 Epidermal Growth Factor Human genes 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 101150027313 Has2 gene Proteins 0.000 description 3
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 239000001768 carboxy methyl cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 239000006071 cream Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000011194 food seasoning agent Nutrition 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 235000011187 glycerol Nutrition 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 239000001863 hydroxypropyl cellulose Substances 0.000 description 3
- 235000010977 hydroxypropyl cellulose Nutrition 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 244000215068 Acacia senegal Species 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- QFOHBWFCKVYLES-UHFFFAOYSA-N Butylparaben Chemical compound CCCCOC(=O)C1=CC=C(O)C=C1 QFOHBWFCKVYLES-UHFFFAOYSA-N 0.000 description 2
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 2
- 206010061762 Chondropathy Diseases 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 101800003838 Epidermal growth factor Proteins 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- 229920000084 Gum arabic Polymers 0.000 description 2
- 101150085382 HAS1 gene Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 239000012980 RPMI-1640 medium Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 235000010489 acacia gum Nutrition 0.000 description 2
- 239000000205 acacia gum Substances 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 235000008429 bread Nutrition 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 2
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 208000015100 cartilage disease Diseases 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 235000015218 chewing gum Nutrition 0.000 description 2
- 229940112822 chewing gum Drugs 0.000 description 2
- OSASVXMJTNOKOY-UHFFFAOYSA-N chlorobutanol Chemical compound CC(C)(O)C(Cl)(Cl)Cl OSASVXMJTNOKOY-UHFFFAOYSA-N 0.000 description 2
- 230000001332 colony forming effect Effects 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 238000010411 cooking Methods 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 229940116977 epidermal growth factor Drugs 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000003925 fat Substances 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 230000037406 food intake Effects 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 229940014259 gelatin Drugs 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 239000007937 lozenge Substances 0.000 description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 201000008482 osteoarthritis Diseases 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 229910000160 potassium phosphate Inorganic materials 0.000 description 2
- 235000011009 potassium phosphates Nutrition 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 description 2
- 235000021067 refined food Nutrition 0.000 description 2
- 235000015067 sauces Nutrition 0.000 description 2
- 235000020374 simple syrup Nutrition 0.000 description 2
- 235000002639 sodium chloride Nutrition 0.000 description 2
- JUJBNYBVVQSIOU-UHFFFAOYSA-M sodium;4-[2-(4-iodophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate Chemical compound [Na+].C1=CC([N+](=O)[O-])=CC=C1N1[N+](C=2C=CC(I)=CC=2)=NC(C=2C(=CC(=CC=2)S([O-])(=O)=O)S([O-])(=O)=O)=N1 JUJBNYBVVQSIOU-UHFFFAOYSA-M 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 229960004793 sucrose Drugs 0.000 description 2
- 235000020357 syrup Nutrition 0.000 description 2
- 239000006188 syrup Substances 0.000 description 2
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 1
- MASUWVVNWALEEM-UHFFFAOYSA-M 1-methoxy-5-methylphenazin-5-ium;methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2N=C3C(OC)=CC=CC3=[N+](C)C2=C1 MASUWVVNWALEEM-UHFFFAOYSA-M 0.000 description 1
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- SPSPIUSUWPLVKD-UHFFFAOYSA-N 2,3-dibutyl-6-methylphenol Chemical compound CCCCC1=CC=C(C)C(O)=C1CCCC SPSPIUSUWPLVKD-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 241000609240 Ambelania acida Species 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 238000011725 BALB/c mouse Methods 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 208000032170 Congenital Abnormalities Diseases 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 206010061619 Deformity Diseases 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 239000004278 EU approved seasoning Substances 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 240000008620 Fagopyrum esculentum Species 0.000 description 1
- 235000009419 Fagopyrum esculentum Nutrition 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 239000004354 Hydroxyethyl cellulose Substances 0.000 description 1
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 102100037850 Interferon gamma Human genes 0.000 description 1
- 108010074328 Interferon-gamma Proteins 0.000 description 1
- 102000013462 Interleukin-12 Human genes 0.000 description 1
- 108010065805 Interleukin-12 Proteins 0.000 description 1
- 241001125831 Istiophoridae Species 0.000 description 1
- 208000003947 Knee Osteoarthritis Diseases 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- MJGJPHUUDJCUEC-UHFFFAOYSA-N OC(=O)C[Ca] Chemical compound OC(=O)C[Ca] MJGJPHUUDJCUEC-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 238000011530 RNeasy Mini Kit Methods 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical class [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 1
- 239000003655 absorption accelerator Substances 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 239000010905 bagasse Substances 0.000 description 1
- LFYJSSARVMHQJB-QIXNEVBVSA-N bakuchiol Chemical compound CC(C)=CCC[C@@](C)(C=C)\C=C\C1=CC=C(O)C=C1 LFYJSSARVMHQJB-QIXNEVBVSA-N 0.000 description 1
- 235000021168 barbecue Nutrition 0.000 description 1
- 229960000686 benzalkonium chloride Drugs 0.000 description 1
- CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical compound [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000015895 biscuits Nutrition 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000005282 brightening Methods 0.000 description 1
- 235000010354 butylated hydroxytoluene Nutrition 0.000 description 1
- 229940067596 butylparaben Drugs 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000010216 calcium carbonate Nutrition 0.000 description 1
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 235000014171 carbonated beverage Nutrition 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 229960004926 chlorobutanol Drugs 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 235000021270 cold food Nutrition 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 229940099112 cornstarch Drugs 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 235000014048 cultured milk product Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 235000021438 curry Nutrition 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 235000019700 dicalcium phosphate Nutrition 0.000 description 1
- 235000013681 dietary sucrose Nutrition 0.000 description 1
- OPGYRRGJRBEUFK-UHFFFAOYSA-L disodium;diacetate Chemical compound [Na+].[Na+].CC([O-])=O.CC([O-])=O OPGYRRGJRBEUFK-UHFFFAOYSA-L 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 229960001617 ethyl hydroxybenzoate Drugs 0.000 description 1
- 235000010228 ethyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004403 ethyl p-hydroxybenzoate Substances 0.000 description 1
- NUVBSKCKDOMJSU-UHFFFAOYSA-N ethylparaben Chemical compound CCOC(=O)C1=CC=C(O)C=C1 NUVBSKCKDOMJSU-UHFFFAOYSA-N 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 235000001727 glucose Nutrition 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 description 1
- 229920003132 hydroxypropyl methylcellulose phthalate Polymers 0.000 description 1
- 229940031704 hydroxypropyl methylcellulose phthalate Drugs 0.000 description 1
- 229940071676 hydroxypropylcellulose Drugs 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 235000008446 instant noodles Nutrition 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 235000015094 jam Nutrition 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229940031703 low substituted hydroxypropyl cellulose Drugs 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 235000011147 magnesium chloride Nutrition 0.000 description 1
- 235000019359 magnesium stearate Nutrition 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 239000008268 mayonnaise Substances 0.000 description 1
- 235000010746 mayonnaise Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 235000010981 methylcellulose Nutrition 0.000 description 1
- 229960002216 methylparaben Drugs 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016046 other dairy product Nutrition 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- WVDDGKGOMKODPV-ZQBYOMGUSA-N phenyl(114C)methanol Chemical compound O[14CH2]C1=CC=CC=C1 WVDDGKGOMKODPV-ZQBYOMGUSA-N 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229940068968 polysorbate 80 Drugs 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 235000019422 polyvinyl alcohol Nutrition 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 235000021395 porridge Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 235000010232 propyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004405 propyl p-hydroxybenzoate Substances 0.000 description 1
- 235000013772 propylene glycol Nutrition 0.000 description 1
- 229960003415 propylparaben Drugs 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 235000012045 salad Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 235000011888 snacks Nutrition 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000015424 sodium Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 235000011083 sodium citrates Nutrition 0.000 description 1
- 235000017454 sodium diacetate Nutrition 0.000 description 1
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 description 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 1
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
- 235000014214 soft drink Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 235000013547 stew Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0656—Adult fibroblasts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/30—Dietetic or nutritional methods, e.g. for losing weight
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/70—Non-animal cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Epidemiology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Rheumatology (AREA)
- Dermatology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Physical Education & Sports Medicine (AREA)
- Polymers & Plastics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Birds (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
本發明之目的係提供纖維母細胞增殖促進用組成物及/或玻尿酸合成酶基因表現促進用組成物。
本發明提供含有植物乳桿菌L-137之纖維母細胞增殖促進用組成物及/或玻尿酸合成酶基因表現促進用組成物。
Description
本發明係關於玻尿酸產生促進用組成物，更詳細而言，係關於含有植物乳桿菌(Lactobacillus plantarum)L-137之玻尿酸產生促進用組成物。
關於植物乳桿菌L-137，已知具有預防感冒等各種功能(專利文獻1至6及非專利文獻1至10)，然而關於植物乳桿菌L-137之纖維母細胞增殖促進作用及玻尿酸合成酶基因表現促進作用，一切均未知，且未暗示。
[專利文獻1]日本特開2010-95465號公報
[專利文獻2]日本特開平10-167972號公報
[專利文獻3]WO2014/199448號小冊子
[專利文獻4]WO2008/018143號小冊子
[專利文獻5]WO2004/084923號小冊子
[專利文獻6]WO2004/084922號小冊子
[非專利文獻1] Iwasaki. et al, Oral Health Prev Dent., 2016; 14(3): 207-14.
[非專利文獻2] Hatano. Et al, Int Immunopharmacol., 2015; Apr; 25(2): 321-31
[非專利文獻3] Hirose. et al, J Nutr Sci., 2013 Dec 6; 2: e39
[非專利文獻4] Fujiki. et al, Biosci Biotechnol Biochem., 2012; 76(5): 918-22.
[非專利文獻5] Arimori. et al, Immunopharmacol Immunotoxicol. 2012 Dec; 34(6): 937-43
[非專利文獻6] Hirose. et al, Microbiol Immunol. 2010 Mar; 54(3): 143-51.
[非專利文獻7] Maeda. Et al, Int Immunopharmacol. 2009 Aug; 9(9): 1122-5.
[非專利文獻8] Hirose. et al, J Nutr. 2006 Dec; 136(12): 3069-73.
[非專利文獻9] Murosaki. et al, Cancer Immunol Immunother. 2000 Jun; 49(3): 157-64.
[非專利文獻10] Murosaki. et al, J Allergy Clin Immunol. 1998Jul; 102(1): 57-64.
本發明之目的係提供纖維母細胞增殖促進用組成物及/或玻尿酸合成酶基因表現促進用組成物。
本發明人等發現含有植物乳桿菌L-137之組成物具有纖維母細胞增殖促進作用及/或玻尿酸合成酶基因表現促進作用，進一步重覆檢討而完成本發明。
亦即，本發明如以下之說明。
[1]一種纖維母細胞增殖促進用之組成物，係含有植物乳桿菌L-137。
[2]一種玻尿酸合成酶基因表現促進用之組成物，係含有植物乳桿菌L-137。
[3]如前述[1]或[2]記載之組成物，係用於促進玻尿酸產生。
[4]如前述[1]或[2]記載之組成物，係用於皮膚疾病或關節炎之預防、改善或治療。
[5]一種含有玻尿酸之組成物之製造方法，係具有使(1)(a)植物乳桿菌L-137及(b)免疫細胞或免疫細胞產生成分，與(2)纖維母細胞接觸之步驟。
[6]一種含有玻尿酸之組成物，係由前述[5]之製造方法所製造者。
[7]如前述[1]至[4]、及[6]中任一項記載之組成物，係飲食品。
[8]如前述[7]記載之組成物，其中該飲食品為食品添加物或補充劑。
[9]一種促進纖維母細胞增殖及/或玻尿酸合成酶基因表現之方法，係包含將植物乳桿菌L-137之有效量投與攝取者的步驟。
[9-2]如前述[9]記載之方法，其中植物乳桿菌L-137係促進玻尿酸產生。
[9-3]如前述[9]或[9-2]記載之方法，其中植物乳桿菌L-137係預防、改善或治療皮膚疾病或關節炎。
[9-4]如前述[9]至[9-3]中任一項記載之方法，其中植物乳桿菌L-137係包含於飲食品中。
[9-5]如前述[9-4]記載之方法，其中該飲食品為食品添加物或補充劑。
[10]一種植物乳桿菌L-137，係用於促進纖維母細胞增殖及/或玻尿酸合成酶基因表現。
[10-2]如前述[10]記載之植物乳桿菌L-137，其中該植物乳桿菌L-137係用於促進玻尿酸產生。
[10-3]如前述[10]或[10-2]記載之植物乳桿菌L-137，其中該植物乳桿菌L-137係用於預防、改善或治療皮膚疾病或關節炎。
[10-4]如前述[10]至[10-3]中任一項記載之植物乳桿菌L-137，其中該植物乳桿菌L-137係包含於飲食品中。
[10-5]如前述[10-4]記載之植物乳桿菌L-137，其中該飲食品為食品添加物或補充劑。
[11]一種植物乳桿菌L-137之用途，係用於製造纖維母細胞增殖及/或玻尿酸合成酶基因表現促進用醫藥。
[11-2]如前述[11]記載之用途，其中該植物乳桿菌L-137係促進玻尿酸產生。
[11-3]如前述[11]或[11-2]記載之用途，其中該植物乳桿菌L-137係預防、改善或治療皮膚疾病或關節炎。
[11-4]如前述[11]至[11-3]中任一項記載之用途，其中該植物乳桿菌L-137係包含於飲食品中。
[11-5]如前述[11-4]記載之用途，其中該飲食品為食品添加物或補充劑。
[12]一種植物乳桿菌L-137之用途，係用於促進纖維母細胞增殖及/或玻尿酸合成酶基因表現。
[12-2]如前述[12]記載之用途，其中該植物乳桿菌L-137係促進玻尿酸產生。
[12-3]如前述[12]或[12-2]記載之用途，其中該植物乳桿菌L-137係預防、改善或治療皮膚疾病或關節炎。
[12-4]如前述[12]至[12-3]中任一項記載之用途，其中該植物乳桿菌L-137係包含於飲食品中。
[12-5]如前述[12-4]記載之用途，其中該飲食品為食品添加物或補充劑。
本發明之組成物，較佳可達到一種以上選自下列之效果：(1)促進纖維母細胞增殖之效果、(2)促進例如HAS1或者HAS2等玻尿酸合成酶基因表現之效果、(3)促進玻尿酸產生之效果、(4)預防、改善或治療例如異位性皮膚炎、乾癬等皮膚疾病之效果、(5)預防、改善或治療例 如膝之疼痛、軟骨障礙、變形性膝關節症、軟骨之缺損、軟骨之損傷、或者半月板損傷等關節炎之效果。
第1圖為展示玻尿酸合成酶1(HAS1)基因表現量之圖(學生t-檢定，*p<0.01 vs.比較例1，平均值±標準偏差)。
第2圖為展示玻尿酸合成酶2(HAS2)基因表現量之圖(學生t-檢定，*p<0.01 vs.比較例1，平均值±標準偏差)。
第3圖為展示纖維母細胞增殖活性之圖(學生t-檢定，*p<0.01 vs.比較例2，平均值±標準偏差)。
第4圖為展示玻尿酸產生量之圖(學生t-檢定，*p<0.01 vs.比較例2，平均值±標準偏差)。
本發明提供(1)含有植物乳桿菌L-137之纖維母細胞增殖促進用組成物及/或(2)含有植物乳桿菌L-137之玻尿酸合成酶基因表現促進用組成物(以下亦稱為本發明之組成物)。
本發明之組成物含有植物乳桿菌L-137。植物乳桿菌L-137係寄託在日本獨立行政法人製品評價技術基盤機構 專利生物寄託中心(地址：日本郵遞區號292-0818千葉縣木更津市上總鎌足2-5-8120號室)，受託編號為FERMBP-08607號(平成7年11月30日從所寄託之FERM P-15317號移管)。再者，即使為植物乳桿菌L-137之變異株，具備植物乳桿菌L-137之特徴者亦為植物乳桿 菌L-137之範疇。
本發明之組成物除植物乳桿菌L-137之外，亦可含有其他成分。亦即，本發明之組成物可藉由將植物乳桿菌L-137及視需要期望之其他成分混合、攪拌等而製造。
植物乳桿菌L-137以外之成分，只要不失去本發明之效果，即無特別限定，例如可使用醫藥、藥學或食品等之領域中已知的任何成分。就植物乳桿菌L-137以外之成分而言，具體言之，可列舉免疫細胞、免疫細胞產生成分、或周知之添加劑等。本發明之組成物藉由含有免疫細胞及/或免疫細胞產生成分，可具有更優良之纖維母細胞增殖促進活性及/或玻尿酸產生促進活性。
在本揭示中，就免疫細胞而言，可列舉B細胞或T細胞等淋巴球、或巨噬細胞等。此等細胞可從哺乳類或鳥類等之血液、或脾臟等，藉由周知之方法而得到，又，亦可購入市售品。免疫細胞產生成分只要為免疫細胞所產生之成分即可，並無特別限定，然而以藉由植物乳桿菌L-137與免疫細胞接觸，免疫細胞中之產生被促進的物質為較佳。就免疫細胞所產生之物質(成分)而言，例如，可列舉IL-12、IFN-γ、TNF-α等細胞激素或各種增殖因子。
本發明之組成物中之植物乳桿菌L-137之含量，若能發揮本發明之效果，即無特別限定，然而可為組成物100質量%中例如約0.001質量%至100質量%之範圍，亦可為1至50質量%之範圍。
本發明之組成物中的免疫細胞之數目，若能發揮本發明之效果，即無特別限定，可為1至1×107細胞/mL。
(植物乳桿菌L-137之取得方法)
植物乳桿菌L-137之培養，可依照周知方法、本身周知之方法或以彼等為基準之方法而進行。植物乳桿菌L-137，例如可藉由在天然培養基、合成培養基、或半合成培養基等培養基中培養而得到。就培養基而言，較佳為使用含有氮源及/或碳源者。就前述氮源而言，例如，可列舉肉萃取物、蛋白腖、麵筋、酪蛋白、酵母萃取物、胺基酸等。就前述碳源而言，例如，可列舉葡萄糖、木糖、果糖、肌醇、麥芽糖、水飴、麴汁、澱粉、甘蔗渣、麩皮、糖蜜、甘油等。此等可使用1種或將2種以上組合而使用。
前述培養基中，除前述氮源及/或碳源外，可再添加1種為無機質之例如硫酸銨、磷酸鉀、氯化鎂、食鹽、鐵、錳、鉬、或各種維生素類等，或將2種以上組合而添加。
在本發明之一態樣中，前述植物乳桿菌L-137之培養溫度，例如，通常為約25至40℃左右，較佳為約27至35℃左右。
在本發明之一態樣中，前述植物乳桿菌L-137之培養時間為約12至48小時左右，亦可進行通氣振盪。又，在本發明之一態樣中，植物乳桿菌L-137之培養，亦可藉由通氣振盪而實施。又，培養基之pH無特別限定，通常為約pH 3至6，較佳為約pH 4至6。
植物乳桿菌L-137之菌體可為活菌體，亦可為死菌體，然而從安定性及操作之容易性等的觀點言之，以使用死菌體為較佳。
培養終了後，可於採收菌體後，調製加熱死菌體，或者亦可不將菌體從培養液一次分離，而將培養液中之菌體加熱成為死菌體，再採收該加熱死菌體。就採收菌體之方法而言，例如在培養液中添加蒸餾水，藉由離心等手段除去上清液，視需要重覆該操作，並藉由離心或過濾等而採收菌體的方法。
植物乳桿菌L-137之加熱死菌體，可將各採收之活菌或含活菌之培養液，藉由加熱處理使其不活化，並藉由噴霧乾燥、凍結乾燥等適當手段進行乾燥而得到。加熱溫度通常為約60至100℃，較佳為約70至90℃。就加熱手段而言，可為使用加熱器之周知手段。加熱時間係在達到期望之溫度後，通常約5至40分鐘，較佳為約10至30分鐘。
如上述之方式所得到之前述死菌體，亦可再進行磨碎、破碎或凍結乾燥處理等而作為死菌體處理物。在本發明中，前述死菌體處理物亦可適用作為死菌體。
(使用方法)
本發明之組成物的投與途徑，並無特別限定，例如，可藉由經口或非經口之任一種途徑投與至哺乳動物等。本發明之組成物在以經口方式投與的情況，係與活體內之免疫細胞接觸，在以非經口方式投與之情況，係與常存在於 皮膚等之免疫細胞接觸，而發揮效果。
植物乳桿菌L-137之投與量或攝取量，在經口或注射投與之情況，可依據攝取者之年齡及體重、症狀、投與時間、劑型、投與方法、藥劑之組合等而決定。例如，設定成人1人(約60kg)每1日，以乾燥死菌體計，較佳攝取約0.5至200mg、更佳約1至100mg、再更佳約2至50mg之植物乳桿菌L-137。或者設定成人1人(約60kg)每1日，換算成活菌，較佳攝取約(5×108)至(2×1011)cfu(Colony forming unit；菌落形成單位)、更佳約(1×109)至(1×1011)cfu之植物乳桿菌L-137。攝取次數可分為1日1次或複數次而進行。
又，在外用塗布之情況，可依照適用之皮膚面積，適宜選擇植物乳桿菌L-137之塗布量，然而相對於適用部位之面積約10cm2，通常該塗布量每1日較佳為約0.01至2.5mg、更佳為約0.02至1mg。可將前述之投與用量每1日1次或分為數次而投與。
在將本發明之組成物經口投與的情況，可為散劑、顆粒劑、丸劑、錠劑、膠囊劑等固體製劑，亦可為糖漿劑等液劑。在製造此等製劑之情況，可依據其製劑型式，使用載劑或添加劑。就載劑或者添加劑而言，例如可列舉賦形劑(聚丙烯酸鈉、聚丙烯酸鈣、羧甲基纖維素、乳糖、糊精、玉米澱粉、結晶纖維素、白糖、氯化鈉、葡萄糖、尿素、澱粉、碳酸鈣、高嶺土、矽酸、磷酸鉀等)、潤滑劑(硬脂酸鎂、蔗糖脂肪酸酯、甘油脂肪酸酯、精製滑 石粉、聚乙二醇等)、崩散劑(羧甲基纖維素鈣、無水磷酸氫鈣、羧甲基纖維素鈉、低取代度羥基丙基纖維素、乾燥澱粉、海藻酸鈉、瓊脂粉末、碳酸氫鈉、碳酸鈣等)、黏合劑(羥基丙基纖維素、阿拉伯膠液、水、乙醇、丙醇、單糖漿、葡萄糖液、澱粉液、明膠溶液、羧甲基纖維素、甲基纖維素、聚乙烯基吡咯啶酮等)、溶解輔助劑(阿拉伯膠、Polysorbate 80等)、吸吸促進劑(月桂基硫酸鈉等)、緩衝劑(磷酸緩衝液、醋酸緩衝液、硼酸緩衝液、碳酸緩衝液、檸檬酸緩衝液、TRIS緩衝液等)、防腐劑(對羥基苯甲酸甲酯、對羥基苯甲酸乙酯、對羥基苯甲酸丙酯、對羥基苯甲酸丁酯、氯丁醇、苯甲醇、苯札氯銨(benzalkonium chloride)、去水醋酸鈉、乙底酸鈉等)、增黏劑(丙二醇、甘油、羥基乙基纖維素、羥基丙基纖維素、聚乙烯醇、聚乙二醇等)、安定化劑(亞硫酸氫鈉、硫代硫酸鈉、乙底酸鈉、檸檬酸鈉、抗壞血酸、二丁基羥基甲苯等)或pH調整劑(鹽酸、氫氧化鈉、磷酸、醋酸等)。視需要亦可用塗布劑(白糖、明膠、羥基丙基纖維素、羥基丙基甲基纖維素酞酸酯等)被覆，又，亦可用2以上之層被覆。
本發明之組成物並無特別限定，例如可列舉飲食品、飼料、醫藥品、醫藥部外品或化妝品等，較佳為飲食品等。
含有本發明之組成物的飲食品，可添加一般飲食品所用的食品添加劑，例如甜味料、著色料、防腐料、增黏安定劑、抗氧化劑、著色料、漂白料、防黴劑、 口香糖膠基、苦味料、酵素、光澤劑、酸味料、調味料、乳化劑、強化劑、製造用劑、香料、香辛料萃取物等。再者，飲食品方面，包含功能性標示食品、特定保健用食品、健康食品、病者用食品。
本發明中較佳飲食品並無特別限定。具體而言，例如可列舉作為食品添加物或所謂營養輔助食品(補充劑)之錠劑、顆粒劑、散劑、飲用劑等。此外，可列舉例如茶飲料、清涼飲料、碳酸飲料、營養飲料、果實飲料、乳酸飲料等飲料；蕎麥麵、烏龍麵、中華麵、速食麵等麵類；飴、糖果、口香糖、巧克力、零食糖類、餅乾、果凍、果醬、乳膏、烘焙食物、麵包等糖果及麵包類；魚糕、火腿、香腸等水產/畜產加工食品；加工乳、發酵乳等乳製品；沙拉油、天婦羅油、乳瑪琳、蛋黃醬、起酥油、鮮奶油、調味醬等油脂及油脂加工食品；醬油、烤肉醬等調味料；咖哩、燉品、蓋飯、粥品、雜炊等烹飪袋裝食品；冰淇淋、果子露、刨冰等冷食等。
就含有本發明之組成物的飼料而言，例如，可列舉牛、馬、豬等家畜用之飼料、雞等家禽用之飼料、魚類等之養殖用之飼料、狗、貓等寵物用之飼料等。本發明之飼料，除於飼料中添加本發明之組成物以外，可使用一般飼料之製造方法進行加工製造。
又，本發明之組成物亦可摻配於醫藥品中使用。醫藥品可將植物乳桿菌L-137與周知之醫藥品添加劑等調配而製造。
又，本發明之組成物亦可調配於醫藥部外品或化妝品中而使用。就化妝品而言，例如，可列舉身體洗淨料、洗手料、洗面料等洗淨料類；或化妝水、乳液、乳膏等基礎化妝料；粉底、補妝料、白粉等化妝用料等化妝料劑型等。
將本發明之組成物調製成飲食品、飼料、醫藥品、醫藥部外品或化妝品之形式的情況，該飲食品、飼料、醫藥品、醫藥部外品或化妝品，於其附加之說明書或其包裝盒等，可根據本發明之組成物之作用，標示1種以上選自下述之要旨：(1)促進纖維母細胞增殖之要旨、(2)促進例如HAS1或HAS2等玻尿酸合成酶基因表現之要旨、(3)促進玻尿酸產生之要旨、(4)對例如異位性皮膚炎、乾癬等皮膚疾病具有預防、改善或治療效果之要旨、(5)對例如膝疼痛、軟骨障害、變形性膝關節症、軟骨缺損、軟骨損傷、或者半月板損傷等關節炎具有預防、改善或治療效果之要旨。
皮膚疾病及關節炎可分別藉由促進纖維母細胞之增殖及/或促進玻尿酸合成酶基因之表現而預防、改善或治療。
本發明之組成物，較佳為可達到一種以上選自下列之效果：(1)促進纖維母細胞增殖之效果，(2)促進例如HAS1或HAS2等玻尿酸合成酶基因表現之效果，(3)促進玻尿酸產生之效果，(4)預防、改善或治療例如異位性皮膚炎、乾癬等皮膚疾病之效果，(5)預防、改善或治療例 如膝之疼痛、軟骨障害、變形性膝關節症、軟骨之缺損、軟骨之損傷、或者半月板損傷等關節炎之效果。
關於是否具有纖維母細胞增殖促進效果，例如超過未添加試料之對照組的纖維母細胞增殖能力時，可判斷為該試料具有纖維母細胞增殖促進效果。
關於是否具有玻尿酸合成酶基因表現促進效果，例如超過未添加試料之對照組的玻尿酸合成酶基因表現能力時，可判斷為該試料具有玻尿酸合成酶基因表現促進效果。
關於是否具有玻尿酸產生促進效果，例如超過未添加試料之對照組的玻尿酸產生能力時，可判斷為該試料具有玻尿酸產生促進效果。
關於是否具有皮膚疾病之改善或治療效果，例如在試料投與前及投與後，在問卷之結果，檢査項目「癢」等中見到顯著差異時，可判定該試料具有皮膚疾病之改善或治療效果。
關於是否具有關節炎之改善或治療效果，例如在試料投與前及投與後，於WOMAC(Western Ontario and McMaster Universities Osteoarthritis Index)評價、SF-36(MOS 36-Item Short-Form Health Survey)、JKOM(Japanese Knee Osteoarthiritis Measure)評價等中，看到顯著差異時，可判斷該試料具有關節炎之改善或治療效果。
本發明提供一種含有玻尿酸之組成物的製造方法，係具有使(1)(a)植物乳桿菌L-137及(b)免疫細胞或免疫細胞產生成分，與(2)纖維母細胞接觸的步驟。
纖維母細胞可為在包含人類之哺乳類等之體內者，亦可為在體外者，然而以於體內為較佳。上述成分(1)與(2)之接觸，可為藉由成分(1)之經口攝取或塗布於皮膚而產生者，亦可為藉由在成分(2)之培養基中添加成分(1)而產生者。
本發明提供依照上述製造方法而製造的含有玻尿酸之組成物(以下，亦稱為本發明之含有玻尿酸之組成物)。
本發明之含有玻尿酸之組成物，可含有上述成分(1)、上述成分(2)、或醫藥、藥學或食品等領域中已知的任何成分。
本發明之含有玻尿酸之組成物，較佳可達到1種以上選自下列之效果：預防、改善或治療例如異位性皮膚炎、乾癬等皮膚疾病之效果；預防、改善或治療例如膝之疼痛、軟骨障害、變形性膝關節症、軟骨之缺損、軟骨之損傷、或半月板損傷等關節炎之效果。
本發明之含有玻尿酸之組成物，可為飲食品、化妝品或醫藥部外品等，然而以飲食品為較佳。在本發明之含有玻尿酸之組成物為飲食品之情況，具體而言，例如，可列舉作為食品添加物或所謂營養輔助食品(補充劑)的錠劑、顆粒劑、散劑、飲用劑等。
以下列舉實施例及試驗例，進一步具體說明本發明，然而本發明不以此等為限。
1. HAS1及HAS2基因表現促進效果之評價
(a)試料之調製(1)
(實施例1)
將為免疫細胞之BALB/c小鼠脾臟細胞2.5×106cells/ml於含有500ng/ml之植物乳桿菌L-137加熱死菌體(HK L-137)且含有10質量%FBS(Hyclone公司製)之RPMI1640培養基(Life Technologies公司製)中，於37℃培養48小時，回收培養後之培養基，得到脾臟細胞培養上清液。將小鼠纖維母細胞(BALB/3T3 clone A31)在含25質量%該脾臟細胞培養上清液且含有2質量%FBS(Hyclone公司製)的D-MEM培養基(Sigma公司製)中，於37℃培養3小時。
(比較例1)
除了將「含有500ng/ml之HK L-137且含有10質量%FBS(Hyclone公司製)的RPMI1640培養基(Life Technologies公司製)」，以「未添加HK L-137之培養基」取代以外，經由與上述實施例同樣之步驟，得到脾臟細胞培養上清液。將小鼠纖維母細胞(BALB/3T3 clone A31)在含25質量%該脾臟細胞培養上清液且含有2質量%FBS(Hyclone公司製)的D-MEM培養基(Sigma公司製)中，於37℃培養3小時。
(陰性對照1)
將小鼠纖維母細胞(BALB/3T3cloneA31)在含有2質量%FBS(Hyclone公司製)之D-MEM培養基(Sigma公司製) 中，於37℃培養3小時。
(陽性對照1)
將小鼠纖維母細胞(BALB/3T3 clone A31)在含10ng/ml之上皮成長因子(EGF；Epidermal Growth Factor)且含有2質量%FBS(Hyclone公司製)之D-MEM培養基(Sigma公司製)中，於37℃培養3小時。
(b)試驗
將培養後之細胞中的RNA使用套組(RNeasy Mini Kit，Qiagen公司)萃取。藉由RT-PCR法，進行總RNA中之HAS1基因及HAS2基因之mRNA表現量的相對定量。求取HAS1及HAS2之mRNA表現量相對於為內部標準之GAPDH之mRNA表現量的比率。使用於測定之引子的序列，如表1所記載。
(HAS1)
將結果示於第1圖。若以陰性對照1之基因表現量當作100%，則比較例1為77%之表現量，實施例1為193% 之表現量。與比較例1相比，實施例1之HAS1基因表現量顯著地為較高值。
(HAS2)
將結果示於第2圖。若以陰性對照1之基因表現量當作100%，則比較例1為132%之表現量，實施例1為242%之表現量。與比較例1相比，實施例1之HAS2基因表現量顯著地為較高值。
2. 纖維母細胞賦活化作用之評價
(a)試料之調製(2)
除小鼠纖維母細胞(BALB/3T3 clone A31)之培養時間從「3小時」變更為「72小時」以外，經由與上述試料之調製(1)同樣的步驟，得到實施例2、比較例2、陰性對照2、及陽性對照2。
(b)試驗
藉由WST-1法測定細胞增殖(代謝)活性。具體而言，將含有500μM WST-1(WAKO公司製)及20μM 1-Methoxy PMS(WAKO公司製)之溶液，添加於(a)之培養液中，並使濃度成為5質量%。測定剛添加後及於37℃培養2小時後之培養基於450nm之吸光度，以吸光度之增加量作為細胞增殖活性。
將結果示於第3圖。以陰性對照之纖維母細胞增殖活性當作100%，則比較例2為110%，實施例2為180%。與比較例2相比，實施例2之纖維母細胞增殖活性顯著地為較高值。
3. 玻尿酸合成促進作用之評價
(a)試料
使用上述實施例2、比較例2、陰性對照2及陽性對照2。
(b)試驗
藉由ELISA套組(BTP-96200，Biotech Trading Partners公司)測定回吸之培養基中的玻尿酸量。
將結果示於第4圖。若以陰性對照之玻尿酸產生量當作100%，則比較例2為133%之玻尿酸產生量，實施例2為244%之玻尿酸產生量。與比較例2相比，實施例2之玻尿酸產生量顯著地為較高值。
從此等結果，顯示植物乳桿菌L-137促進纖維母細胞之玻尿酸合成酶基因表現及/或促進纖維母細胞增殖，並促進玻尿酸產生。
本發明之組成物在作為以促進玻尿酸產生為目的的飲食品、醫藥品、醫藥部外品、化妝品、飼料等上有用。
<110> 日商好侍健康食品股份有限公司
<120> 玻尿酸產生促進用組成物
<130> T09J6219
<150> JP 2017-168930
<151> 2017-09-01
<160> 6
<170> PatentIn version 3.1
<210> 1
<211> 22
<212> DNA
<213> 引子
<400> 1 
<210> 2
<211> 21
<212> DNA
<213> 引子
<400> 2 
<210> 3
<211> 21
<212> DNA
<213> 引子
<400> 3 
<210> 4
<211> 21
<212> DNA
<213> 引子
<400> 4 
<210> 5
<211> 22
<212> DNA
<213> 引子
<400> 5 
<210> 6
<211> 22
<212> DNA
<213> 引子
<400> 6 
Claims (13)
- 一種用以促進纖維母細胞增殖之組成物，係含有植物乳桿菌L-137，其中，前述纖維母細胞增殖係藉由要被投與前述組成物之哺乳類或鳥類之常存於活體內或皮膚之免疫細胞、及/或因植物乳桿菌L-137與前述免疫細胞的接觸而由免疫細胞產生之成分而被促進。
- 一種用以促進玻尿酸合成酶基因表現之組成物，係含有植物乳桿菌L-137，其中，前述玻尿酸合成酶基因表現係藉由要被投與前述組成物之哺乳類或鳥類之常存於活體內或皮膚之免疫細胞、及/或因植物乳桿菌L-137與前述免疫細胞的接觸而由免疫細胞產生之成分而被促進。
- 如申請專利範圍第1或2項所述之組成物，係用於促進玻尿酸產生。
- 如申請專利範圍第1或2項所述之組成物，其係用於皮膚疾病、或關節炎之預防、改善或治療。
- 一種含有玻尿酸之組成物之製造方法，係具有使(1)(a)植物乳桿菌L-137及(b)免疫細胞或免疫細胞產生成分，與(2)纖維母細胞接觸的步驟。
- 如申請專利範圍第5項所述之製造方法，係更藉由要被投與組成物之哺乳類或鳥類之常存於活體內或皮膚之免疫細胞、及/或因植物乳桿菌L-137與前述免疫細胞的接觸而由免疫細胞產生之成分而促進玻尿酸合成酶基因表現。
- 一種含有玻尿酸之組成物，係由如申請專利範圍第5或6項所述之製造方法所製造者。
- 如申請專利範圍第1項、第2項及第7項中任一項所述之組成物，係為飲食品。
- 如申請專利範圍第8項所述之組成物，其中飲食品為食品添加物或補充劑。
- 一種植物乳桿菌L-137之用途，其係用於製造促進纖維母細胞增殖及/或玻尿酸合成酶基因表現用之醫藥。
- 如申請專利範圍第10項所述之用途，其中植物乳桿菌L-137係促進玻尿酸產生。
- 如申請專利範圍第10或11項所述之用途，其中植物乳桿菌L-137係包含於飲食品中。
- 如申請專利範圍第12項所述之用途，其中飲食品為食品添加物或補充劑。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2017168930 | 2017-09-01 | ||
| JP2017-168930 | 2017-09-01 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW201919672A TW201919672A (zh) | 2019-06-01 |
| TWI784045B true TWI784045B (zh) | 2022-11-21 |
Family
ID=65525601
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW107130593A TWI784045B (zh) | 2017-09-01 | 2018-08-31 | 玻尿酸產生促進用組成物 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20200353020A1 (zh) |
| JP (1) | JPWO2019045037A1 (zh) |
| CN (1) | CN111163788A (zh) |
| TW (1) | TWI784045B (zh) |
| WO (1) | WO2019045037A1 (zh) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW202313077A (zh) * | 2021-05-17 | 2023-04-01 | 日商好侍健康食品股份有限公司 | 蝕骨前驅細胞朝蝕骨細胞之分化抑制用組成物及骨代謝改善用組成物 |
| CN115137076A (zh) * | 2022-07-20 | 2022-10-04 | 广州纽缤乐营养科技股份有限公司 | 一种用于补水保湿的食品组合物及其制备方法 |
| CN116004469A (zh) * | 2023-01-17 | 2023-04-25 | 江南大学 | 植物乳植杆菌ccfm1295制备的具有上调皮肤细胞ha合成酶表达的后生元 |
| CN116083315A (zh) * | 2023-01-17 | 2023-05-09 | 江南大学 | 一株具有调控宿主ha含量的植物乳植杆菌ccfm1296及其后生元 |
| CN117338823A (zh) * | 2023-12-06 | 2024-01-05 | 山东百德生物科技有限公司 | 一种动物酵素发酵液及其制备方法与应用 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013245170A (ja) * | 2012-05-23 | 2013-12-09 | Nippon Menaade Keshohin Kk | ヒアルロン酸合成促進剤及び皮膚外用剤 |
| JP2015096476A (ja) * | 2013-11-15 | 2015-05-21 | 株式会社東洋新薬 | コラーゲン産生促進用組成物、コラーゲン吸収促進用組成物、抗肥満用組成物 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4064481B2 (ja) * | 1996-10-11 | 2008-03-19 | ハウスウェルネスフーズ株式会社 | 免疫賦活剤 |
| WO2008018143A1 (fr) * | 2006-08-10 | 2008-02-14 | House Wellness Foods Corporation | Agent hydratant |
| JP2010095465A (ja) * | 2008-10-16 | 2010-04-30 | House Wellness Foods Kk | 乳酸菌含有免疫賦活用組成物 |
| IT1399793B1 (it) * | 2010-01-12 | 2013-05-03 | Giuliani Spa | Procedimento di preparazione di una biomassa comprendente plantaricina e suoi usi in campo medico. |
| JP2017048244A (ja) * | 2016-12-01 | 2017-03-09 | 株式会社東洋新薬 | コラーゲン産生促進用組成物、コラーゲン吸収促進用組成物、抗肥満用組成物 |
-
2018
- 2018-08-31 WO PCT/JP2018/032316 patent/WO2019045037A1/ja active Application Filing
- 2018-08-31 TW TW107130593A patent/TWI784045B/zh active
- 2018-08-31 JP JP2019539656A patent/JPWO2019045037A1/ja active Pending
- 2018-08-31 US US16/640,501 patent/US20200353020A1/en active Pending
- 2018-08-31 CN CN201880044270.9A patent/CN111163788A/zh active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013245170A (ja) * | 2012-05-23 | 2013-12-09 | Nippon Menaade Keshohin Kk | ヒアルロン酸合成促進剤及び皮膚外用剤 |
| JP2015096476A (ja) * | 2013-11-15 | 2015-05-21 | 株式会社東洋新薬 | コラーゲン産生促進用組成物、コラーゲン吸収促進用組成物、抗肥満用組成物 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPWO2019045037A1 (ja) | 2020-10-15 |
| CN111163788A (zh) | 2020-05-15 |
| US20200353020A1 (en) | 2020-11-12 |
| WO2019045037A1 (ja) | 2019-03-07 |
| TW201919672A (zh) | 2019-06-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TWI784045B (zh) | 玻尿酸產生促進用組成物 | |
| JPWO2006093267A1 (ja) | 免疫調節作用を有する発酵組成物 | |
| JP2006320257A (ja) | 海藻発酵エキス、その製造方法およびその用途 | |
| WO2017130859A1 (ja) | 神経細胞死抑制剤 | |
| EP2960327B1 (en) | Leuconostoc mesenteroides as immunostimulation agent | |
| JP2018153182A (ja) | ラクトバチルスプランタルムｔｃｉ３７８、およびそれを用いた脂肪の減少および胃腸機能の改善における使用 | |
| EP3777869A1 (en) | Sleep-promoting composition, and medicinal composition and food and beverage composition using said sleep-promoting composition | |
| TW201840328A (zh) | 肽聚醣辨識蛋白質產生促進用組成物 | |
| JP4565057B2 (ja) | イムノグロブリンａ誘導能の高い新規乳酸菌 | |
| JP4498924B2 (ja) | ラクトバシラス・カゼイ亜種カゼイ増殖促進用組成物 | |
| JP7090288B2 (ja) | 新規乳酸菌、新規乳酸菌を有効成分として含有する自然免疫活性化剤、及び新規乳酸菌を含有する飲食品 | |
| TW201609120A (zh) | 含乳酸菌之腸道屏障功能促進劑 | |
| CN111328285B (zh) | 乳酸菌、来源于该乳酸菌的天然免疫活化剂、感染症预防/治疗剂和饮食品 | |
| JP2006069993A (ja) | 免疫増強剤 | |
| WO2018216735A1 (ja) | メタボリックシンドロームの予防、改善又は治療用組成物 | |
| US20240011067A1 (en) | Limosilactobacillus reuteri strain with high riboflavin production and uses thereof | |
| KR101905322B1 (ko) | 유산균을 포함하는 면역 증강용 조성물 | |
| JP6061530B2 (ja) | Ｎａｓｈ予防治療剤 | |
| KR102244732B1 (ko) | 프로바이오틱 초산균인 아세토박터 파스테리아누스 mglv 및 이의 면역조절 효과 | |
| JP7419404B2 (ja) | 抗老化用組成物 | |
| CN108464509B (zh) | 新型发酵乳酸杆菌在食品领域中的应用 | |
| CN114554874A (zh) | 用于产丁酸菌的益生元组合物 | |
| KR102581570B1 (ko) | 신규 바실러스 벨레젠시스 균주를 포함하는 면역증강용 조성물 | |
| JP2017031109A (ja) | 形質細胞様樹状細胞誘導剤 | |
| EP4342535A1 (en) | Composition for inhibition of differentiation of osteoclast precursor cells into osteoclasts, and composition for improving bone metabolism |