TWI704229B - 核酸定序方法 - Google Patents

核酸定序方法 Download PDF

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Publication number
TWI704229B
TWI704229B TW105115925A TW105115925A TWI704229B TW I704229 B TWI704229 B TW I704229B TW 105115925 A TW105115925 A TW 105115925A TW 105115925 A TW105115925 A TW 105115925A TW I704229 B TWI704229 B TW I704229B
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Taiwan
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luminescence
luminescent
molecules
nucleotides
molecule
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TW105115925A
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Chinese (zh)
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TW201708546A (zh
Inventor
強納森Ｍ 羅斯伯格
傑瑞米 雷克伊
布萊恩 瑞德
施興華
黃海冬
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美商寬騰矽公司
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Priority claimed from US14/821,688 external-priority patent/US9885657B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G01N21/6454—Individual samples arranged in a regular 2D-array, e.g. multiwell plates using an integrated detector array
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/064—Stray light conditioning

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
TW105115925A 2015-05-20 2016-05-20 核酸定序方法 TWI704229B (zh)

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US201562164464P 2015-05-20 2015-05-20
US201562164482P 2015-05-20 2015-05-20
US201562164506P 2015-05-20 2015-05-20
US201562164485P 2015-05-20 2015-05-20
US62/164,482 2015-05-20
US62/164,506 2015-05-20
US62/164,464 2015-05-20
US62/164,485 2015-05-20
US14/821,688 US9885657B2 (en) 2014-08-08 2015-08-07 Integrated device with external light source for probing detecting and analyzing molecules
US14/821,688 2015-08-07

Publications (2)

Publication Number Publication Date
TW201708546A TW201708546A (zh) 2017-03-01
TWI704229B true TWI704229B (zh) 2020-09-11

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Application Number Title Priority Date Filing Date
TW105115925A TWI704229B (zh) 2015-05-20 2016-05-20 核酸定序方法

Country Status (10)

Country Link
EP (2) EP3998471A1 (https=)
JP (2) JP6936736B2 (https=)
KR (1) KR20180008779A (https=)
CN (1) CN108027325A (https=)
AU (2) AU2016264753B2 (https=)
BR (1) BR112017024573B1 (https=)
CA (1) CA2986237A1 (https=)
MX (2) MX2022001187A (https=)
TW (1) TWI704229B (https=)
WO (1) WO2016187580A1 (https=)

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KR20180111999A (ko) 2016-02-17 2018-10-11 테서렉트 헬스, 인코포레이티드 수명 촬상 및 검출 응용을 위한 센서 및 디바이스
LT3443006T (lt) 2016-04-13 2023-10-25 Sanofi Trispecifiniai ir (arba) trivalenčiai rišantieji baltymai
EP4113104A3 (en) 2016-06-01 2023-02-22 Quantum-si Incorporated Integrated device for detecting and analyzing molecules
AU2017377057B2 (en) * 2016-12-16 2022-11-17 Quantum-Si Incorporated Optical coupler and waveguide system
CA3047826A1 (en) 2016-12-22 2018-06-28 Quantum-Si Incorporated Integrated photodetector with direct binning pixel
KR102709865B1 (ko) 2017-05-05 2024-09-26 퀀텀-에스아이 인코포레이티드 생물학적 반응에 있어서 개질된 표면 반응성 및 오손방지 특성을 갖는 기재
US11309684B2 (en) * 2017-05-19 2022-04-19 Københavns Universitet Coherent single photon source
US11994521B2 (en) 2017-06-23 2024-05-28 Nanotemper Technologies Gmbh Methods for measuring inter- and/or intra-molecular interactions
WO2019023257A1 (en) * 2017-07-24 2019-01-31 Quantum-Si Incorporated HIGH INTENSITY MARKED REAGENT COMPOSITIONS AND SEQUENCING METHODS
BR112020000799A2 (pt) * 2017-07-24 2020-07-14 Quantum-Si Incorporated estruturas fotônicas de rejeição óptica
US11162138B2 (en) 2017-10-30 2021-11-02 Pacific Biosciences Of California, Inc. Multi-amplitude modular labeled compounds
CN119364214A (zh) * 2018-06-22 2025-01-24 宽腾矽公司 具有不同检测时间的电荷存储仓的集成光电检测器
WO2020010589A1 (en) * 2018-07-12 2020-01-16 Shenzhen Genorivision Technology Co., Ltd. An apparatus for biopolymer sequencing
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AU2019330042A1 (en) * 2018-08-29 2021-03-11 Quantum-Si Incorporated System and methods for detecting lifetime using photon counting photodetectors
WO2020047272A1 (en) * 2018-08-29 2020-03-05 Quantum-Si Incorporated Sample well fabrication techniques and structures for integrated sensor devices
US12179204B2 (en) 2018-12-03 2024-12-31 Quantum-Si Incorporated Sample well fabrication techniques and structures for integrated sensor
CN111333605B (zh) * 2018-12-18 2022-07-15 中国科学院大连化学物理研究所 一种高亮度、高稳定性的细胞膜碳酸酐酶ⅸ荧光探针
TW202041847A (zh) 2019-01-03 2020-11-16 美商寬騰矽公司 用於傳遞光至光子元件之陣列的光學波導及耦合器
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US11613576B2 (en) 2019-04-09 2023-03-28 Sanofi Trispecific binding proteins, methods, and uses thereof
CN110066723A (zh) * 2019-05-05 2019-07-30 京东方科技集团股份有限公司 基因测序芯片、设备、制造方法
MX2022001614A (es) * 2019-08-08 2022-05-26 Quantum Si Inc Eficiencia mejorada de recolección de emisiones en dispositivos ópticos integrados.
US11712715B2 (en) 2019-10-11 2023-08-01 Quantum-Si Incorporated Surface modification in the vapor phase
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CN114729890A (zh) * 2019-10-17 2022-07-08 C2感官有限公司 用于感测和/或认证的发光成像
AU2021208557A1 (en) 2020-01-14 2022-09-01 Quantum-Si Incorporated Sensor for lifetime plus spectral characterization
WO2021146473A1 (en) 2020-01-14 2021-07-22 Quantum-Si Incorporated Integrated sensor for lifetime characterization
TW202147591A (zh) 2020-03-02 2021-12-16 美商寬騰矽公司 用於多維信號分析之整合感應器
KR20220165754A (ko) 2020-04-08 2022-12-15 퀀텀-에스아이 인코포레이티드 스큐가 감소된 통합 센서
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WO2022263644A1 (en) * 2021-06-17 2022-12-22 Roche Diagnostics Gmbh Thermostable affinity polypeptides
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JP2018521308A (ja) 2018-08-02
MX2017014821A (es) 2018-06-27
WO2016187580A1 (en) 2016-11-24
MX392170B (es) 2025-03-21
AU2016264753A1 (en) 2017-12-14
CN108027325A (zh) 2018-05-11
JP7339302B2 (ja) 2023-09-05
AU2021282471B2 (en) 2024-01-04
CA2986237A1 (en) 2016-11-24
AU2016264753B2 (en) 2021-09-09
EP3298389A1 (en) 2018-03-28
TW201708546A (zh) 2017-03-01
BR112017024573A2 (pt) 2018-07-31
BR112017024573B1 (pt) 2024-03-05
KR20180008779A (ko) 2018-01-24
HK1252483A1 (zh) 2019-05-24
EP3998471A1 (en) 2022-05-18
EP3298389B1 (en) 2021-09-29
AU2021282471A1 (en) 2022-01-06
JP2021192044A (ja) 2021-12-16
JP6936736B2 (ja) 2021-09-22
MX2022001187A (es) 2023-01-04

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