CN108027325A - 采用时间分辨发光确定核酸序列的方法 - Google Patents

采用时间分辨发光确定核酸序列的方法 Download PDF

Info

Publication number
CN108027325A
CN108027325A CN201680040436.0A CN201680040436A CN108027325A CN 108027325 A CN108027325 A CN 108027325A CN 201680040436 A CN201680040436 A CN 201680040436A CN 108027325 A CN108027325 A CN 108027325A
Authority
CN
China
Prior art keywords
nucleotide
luminescent
luminescent marking
type
molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201680040436.0A
Other languages
English (en)
Chinese (zh)
Inventor
乔纳森·M·罗斯伯格
杰瑞米·拉基
布莱恩·瑞德
施兴华
黃海冬
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quantum Si Inc
Original Assignee
Quantum Si Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US14/821,688 external-priority patent/US9885657B2/en
Application filed by Quantum Si Inc filed Critical Quantum Si Inc
Publication of CN108027325A publication Critical patent/CN108027325A/zh
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6408Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6452Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G01N21/6454Individual samples arranged in a regular 2D-array, e.g. multiwell plates using an integrated detector array
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/648Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N2021/6482Sample cells, cuvettes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769Measurement method of reaction-produced change in sensor
    • G01N2021/7786Fluorescence
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00Features of devices classified in G01N21/00
    • G01N2201/06Illumination; Optics
    • G01N2201/064Stray light conditioning

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201680040436.0A 2015-05-20 2016-05-20 采用时间分辨发光确定核酸序列的方法 Pending CN108027325A (zh)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US201562164464P 2015-05-20 2015-05-20
US201562164482P 2015-05-20 2015-05-20
US201562164506P 2015-05-20 2015-05-20
US201562164485P 2015-05-20 2015-05-20
US62/164,482 2015-05-20
US62/164,506 2015-05-20
US62/164,464 2015-05-20
US62/164,485 2015-05-20
US14/821,688 US9885657B2 (en) 2014-08-08 2015-08-07 Integrated device with external light source for probing detecting and analyzing molecules
US14/821,688 2015-08-07
PCT/US2016/033616 WO2016187580A1 (en) 2015-05-20 2016-05-20 Method of determining the sequence of a nuclear acid using time resolved luminescence

Publications (1)

Publication Number Publication Date
CN108027325A true CN108027325A (zh) 2018-05-11

Family

ID=57320962

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680040436.0A Pending CN108027325A (zh) 2015-05-20 2016-05-20 采用时间分辨发光确定核酸序列的方法

Country Status (10)

Country Link
EP (2) EP3998471A1 (https=)
JP (2) JP6936736B2 (https=)
KR (1) KR20180008779A (https=)
CN (1) CN108027325A (https=)
AU (2) AU2016264753B2 (https=)
BR (1) BR112017024573B1 (https=)
CA (1) CA2986237A1 (https=)
MX (2) MX2022001187A (https=)
TW (1) TWI704229B (https=)
WO (1) WO2016187580A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110066723A (zh) * 2019-05-05 2019-07-30 京东方科技集团股份有限公司 基因测序芯片、设备、制造方法

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3471402B1 (en) 2014-08-08 2023-05-31 Quantum-Si Incorporated Integrated device for temporal binning of received photons
RS62437B1 (sr) 2015-10-25 2021-11-30 Sanofi Sa Trispecifični i/ili trivalentni vezujući proteini za prevenciju ili lečenje hiv infekcije
KR20180111999A (ko) 2016-02-17 2018-10-11 테서렉트 헬스, 인코포레이티드 수명 촬상 및 검출 응용을 위한 센서 및 디바이스
LT3443006T (lt) 2016-04-13 2023-10-25 Sanofi Trispecifiniai ir (arba) trivalenčiai rišantieji baltymai
EP4113104A3 (en) 2016-06-01 2023-02-22 Quantum-si Incorporated Integrated device for detecting and analyzing molecules
AU2017377057B2 (en) * 2016-12-16 2022-11-17 Quantum-Si Incorporated Optical coupler and waveguide system
CA3047826A1 (en) 2016-12-22 2018-06-28 Quantum-Si Incorporated Integrated photodetector with direct binning pixel
KR102709865B1 (ko) 2017-05-05 2024-09-26 퀀텀-에스아이 인코포레이티드 생물학적 반응에 있어서 개질된 표면 반응성 및 오손방지 특성을 갖는 기재
US11309684B2 (en) * 2017-05-19 2022-04-19 Københavns Universitet Coherent single photon source
US11994521B2 (en) 2017-06-23 2024-05-28 Nanotemper Technologies Gmbh Methods for measuring inter- and/or intra-molecular interactions
WO2019023257A1 (en) * 2017-07-24 2019-01-31 Quantum-Si Incorporated HIGH INTENSITY MARKED REAGENT COMPOSITIONS AND SEQUENCING METHODS
BR112020000799A2 (pt) * 2017-07-24 2020-07-14 Quantum-Si Incorporated estruturas fotônicas de rejeição óptica
US11162138B2 (en) 2017-10-30 2021-11-02 Pacific Biosciences Of California, Inc. Multi-amplitude modular labeled compounds
CN119364214A (zh) * 2018-06-22 2025-01-24 宽腾矽公司 具有不同检测时间的电荷存储仓的集成光电检测器
WO2020010589A1 (en) * 2018-07-12 2020-01-16 Shenzhen Genorivision Technology Co., Ltd. An apparatus for biopolymer sequencing
CA3105715A1 (en) 2018-07-13 2020-01-16 Quantum-Si Incorporated Biconjugatable labels and methods of use
AU2019330042A1 (en) * 2018-08-29 2021-03-11 Quantum-Si Incorporated System and methods for detecting lifetime using photon counting photodetectors
WO2020047272A1 (en) * 2018-08-29 2020-03-05 Quantum-Si Incorporated Sample well fabrication techniques and structures for integrated sensor devices
US12179204B2 (en) 2018-12-03 2024-12-31 Quantum-Si Incorporated Sample well fabrication techniques and structures for integrated sensor
CN111333605B (zh) * 2018-12-18 2022-07-15 中国科学院大连化学物理研究所 一种高亮度、高稳定性的细胞膜碳酸酐酶ⅸ荧光探针
TW202041847A (zh) 2019-01-03 2020-11-16 美商寬騰矽公司 用於傳遞光至光子元件之陣列的光學波導及耦合器
WO2020154546A1 (en) 2019-01-23 2020-07-30 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
US11613576B2 (en) 2019-04-09 2023-03-28 Sanofi Trispecific binding proteins, methods, and uses thereof
MX2022001614A (es) * 2019-08-08 2022-05-26 Quantum Si Inc Eficiencia mejorada de recolección de emisiones en dispositivos ópticos integrados.
US11712715B2 (en) 2019-10-11 2023-08-01 Quantum-Si Incorporated Surface modification in the vapor phase
EP4045898A4 (en) * 2019-10-17 2024-01-10 C2Sense, Inc. LUMINESCENCE IMAGING FOR CAPTURE
CN114729890A (zh) * 2019-10-17 2022-07-08 C2感官有限公司 用于感测和/或认证的发光成像
AU2021208557A1 (en) 2020-01-14 2022-09-01 Quantum-Si Incorporated Sensor for lifetime plus spectral characterization
WO2021146473A1 (en) 2020-01-14 2021-07-22 Quantum-Si Incorporated Integrated sensor for lifetime characterization
TW202147591A (zh) 2020-03-02 2021-12-16 美商寬騰矽公司 用於多維信號分析之整合感應器
KR20220165754A (ko) 2020-04-08 2022-12-15 퀀텀-에스아이 인코포레이티드 스큐가 감소된 통합 센서
US20220049298A1 (en) * 2020-08-14 2022-02-17 Siphox, Inc. Tagged-base dna sequencing readout on waveguide surfaces
GB2599729A (en) * 2020-10-12 2022-04-13 Sumitomo Chemical Co Method comprising light emitting marker
CA3199745A1 (en) * 2020-10-27 2022-05-05 Quantum-Si Incorporated Calibration of single-molecule detection system
WO2022263644A1 (en) * 2021-06-17 2022-12-22 Roche Diagnostics Gmbh Thermostable affinity polypeptides
WO2023275738A1 (en) * 2021-06-28 2023-01-05 Ecole Polytechnique Federale De Lausanne (Epfl) Method and device for performing spectrally and temporally resolved spectroscopy of single photon emission in a quantum device
WO2023032996A1 (ja) 2021-08-31 2023-03-09 富士フイルム株式会社 化合物及びこれを用いた標識生体物質
WO2023114209A1 (en) * 2021-12-14 2023-06-22 Quantum-Si Incorporated Methods for loading and data acquisition
WO2023137051A2 (en) 2022-01-12 2023-07-20 Quantum-Si Incorporated Integrated sensor

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993006482A1 (en) * 1991-09-16 1993-04-01 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes
EP0710668A2 (en) * 1994-07-18 1996-05-08 Becton, Dickinson and Company Covalent attachment of thiazole orange to oligonucleotides for use in nucleic acid detection
CN1226928A (zh) * 1996-05-31 1999-08-25 米凯尔·库比斯塔 核酸分析的探针
CN1358868A (zh) * 2000-11-29 2002-07-17 株式会社岛津制作所 核酸序列测定方法
CN104419765A (zh) * 2013-08-27 2015-03-18 横河电机株式会社 核酸序列测定方法及装置、核酸序列测定用元件及其制造方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
WO2003020734A2 (en) 2001-08-29 2003-03-13 Amersham Biosciences Corp Labeled nucleoside polyphosphates
JP2007033159A (ja) * 2005-07-25 2007-02-08 Assay Corp 蛍光分子で標識された測定対象物質
JP2010066212A (ja) * 2008-09-12 2010-03-25 Univ Of Tokyo 測定装置及び観察対象の測定方法
US9670243B2 (en) * 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993006482A1 (en) * 1991-09-16 1993-04-01 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes
EP0710668A2 (en) * 1994-07-18 1996-05-08 Becton, Dickinson and Company Covalent attachment of thiazole orange to oligonucleotides for use in nucleic acid detection
CN1226928A (zh) * 1996-05-31 1999-08-25 米凯尔·库比斯塔 核酸分析的探针
CN1358868A (zh) * 2000-11-29 2002-07-17 株式会社岛津制作所 核酸序列测定方法
CN104419765A (zh) * 2013-08-27 2015-03-18 横河电机株式会社 核酸序列测定方法及装置、核酸序列测定用元件及其制造方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
应立明: "单分子的光学检测及应用", 《大学化学》 *
肖树雄: "《中药现代检验新技术》", 31 October 2002 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110066723A (zh) * 2019-05-05 2019-07-30 京东方科技集团股份有限公司 基因测序芯片、设备、制造方法

Also Published As

Publication number Publication date
TWI704229B (zh) 2020-09-11
JP2018521308A (ja) 2018-08-02
MX2017014821A (es) 2018-06-27
WO2016187580A1 (en) 2016-11-24
MX392170B (es) 2025-03-21
AU2016264753A1 (en) 2017-12-14
JP7339302B2 (ja) 2023-09-05
AU2021282471B2 (en) 2024-01-04
CA2986237A1 (en) 2016-11-24
AU2016264753B2 (en) 2021-09-09
EP3298389A1 (en) 2018-03-28
TW201708546A (zh) 2017-03-01
BR112017024573A2 (pt) 2018-07-31
BR112017024573B1 (pt) 2024-03-05
KR20180008779A (ko) 2018-01-24
HK1252483A1 (zh) 2019-05-24
EP3998471A1 (en) 2022-05-18
EP3298389B1 (en) 2021-09-29
AU2021282471A1 (en) 2022-01-06
JP2021192044A (ja) 2021-12-16
JP6936736B2 (ja) 2021-09-22
MX2022001187A (es) 2023-01-04

Similar Documents

Publication Publication Date Title
JP7339302B2 (ja) 時間分解発光を使用して核酸の配列を決定するための方法
US11970729B2 (en) Methods for nucleic acid sequencing
JP7581154B2 (ja) 分子の探索、検出、および解析のための外部光源を備える集積装置
AU2021345360A9 (en) Methods to minimize photodamage during nucleic acid and peptide sequencing
US20250011840A1 (en) Methods for nucleic acid sequencing
HK40075256A (en) Method of determining the sequence of a nucleic acid using time resolved luminescence
HK1252483B (en) Method of determining the sequence of a nucleic acid using time resolved luminescence

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination