TWI513821B - 鑑定及區別多價運輸熱疫苗之病毒成份之組合物及方法 - Google Patents
鑑定及區別多價運輸熱疫苗之病毒成份之組合物及方法 Download PDFInfo
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- C—CHEMISTRY; METALLURGY
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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| CN106906307A (zh) * | 2017-03-10 | 2017-06-30 | 中国农业科学院特产研究所 | 牛副流感病毒3型病毒nano‑PCR检测试剂盒及其制备方法 |
| CN113913461A (zh) * | 2021-11-15 | 2022-01-11 | 贵州大学 | 牛病毒性腹泻e0-e2基因重组腺病毒疫苗构建方法 |
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| NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
| US3817837A (en) | 1971-05-14 | 1974-06-18 | Syva Corp | Enzyme amplification assay |
| US3939350A (en) | 1974-04-29 | 1976-02-17 | Board Of Trustees Of The Leland Stanford Junior University | Fluorescent immunoassay employing total reflection for activation |
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4275149A (en) | 1978-11-24 | 1981-06-23 | Syva Company | Macromolecular environment control in specific receptor assays |
| US4277437A (en) | 1978-04-05 | 1981-07-07 | Syva Company | Kit for carrying out chemically induced fluorescence immunoassay |
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
| US4973679A (en) | 1981-03-27 | 1990-11-27 | University Patents, Inc. | Process for oligonucleo tide synthesis using phosphormidite intermediates |
| US4415732A (en) | 1981-03-27 | 1983-11-15 | University Patents, Inc. | Phosphoramidite compounds and processes |
| DE3329892A1 (de) | 1983-08-18 | 1985-03-07 | Köster, Hubert, Prof. Dr., 2000 Hamburg | Verfahren zur herstellung von oligonucleotiden |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| FR2596761B1 (fr) | 1986-04-08 | 1988-05-20 | Commissariat Energie Atomique | Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides |
| US4992051A (en) * | 1987-11-03 | 1991-02-12 | Novagene, Inc. | Infectious bovine rhinotracheitis virus mutants, methods for the production of same and methods for the use of same |
| US5491225A (en) | 1991-12-19 | 1996-02-13 | Hoffmann-La Roche Inc. | PCR primers for detection of legionella species and methods for controlling visual intensity in hybridization assays |
| US5279721A (en) | 1993-04-22 | 1994-01-18 | Peter Schmid | Apparatus and method for an automated electrophoresis system |
| EP0968722B1 (en) | 1994-05-10 | 2007-06-20 | Wyeth | Improved modified live BRSV vaccine |
| FR2751229B1 (fr) * | 1996-07-19 | 1998-11-27 | Rhone Merieux | Formule de vaccin polynucleotidique notamment contre la pathologie respiratoire des bovins |
| EP1104676A1 (en) * | 1999-11-30 | 2001-06-06 | Boehringer Ingelheim Vetmedica Gmbh | Safe attenuated bovine viral diarrhea viruses for use in pregnant cows |
| DE60139319D1 (en) * | 2000-06-23 | 2009-09-03 | Intervet Int Bv | Us |
| EP2180064A3 (en) * | 2000-10-18 | 2010-08-11 | Pharmasset, Inc. | Multiplex quantification of nucleic acids in diseased cells |
| WO2004073737A1 (en) * | 2003-02-19 | 2004-09-02 | Merial Limited | Vaccination or immunization using a prime-boost regimen against brsv, bhv-1, bvdv, bpi-3 |
| PL1727562T3 (pl) | 2004-03-17 | 2008-10-31 | Pharmacia & Upjohn Co Llc | Sposób szczepienia przeciw zakażeniu jąder wirusem BVD |
| US20090068223A1 (en) | 2005-11-15 | 2009-03-12 | Boehringer Ingelheim Vetmedica, Inc. | Combination vaccine comprising an attenuated bovine viral diarrhea virus |
| WO2007094854A2 (en) | 2005-12-14 | 2007-08-23 | The Board Of Regents Of The University Of Oklahoma | Rna virus vaccines and methods |
| US8354514B2 (en) * | 2007-01-12 | 2013-01-15 | Lawrence Livermore National Security, Llc | Multiplex detection of agricultural pathogens |
| CN101696453B (zh) * | 2009-11-02 | 2012-01-25 | 东北农业大学 | 检测牛病毒性腹泻病毒逆转录环介导等温扩增反应试剂盒的制备方法 |
| PT2658570T (pt) * | 2010-12-27 | 2019-02-08 | Elanco Us Inc | Composições e métodos vacinais contra o vírus da diarreia viral bovina tipo 1b |
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| Title |
|---|
| Willoughby K et al., "Development of a real time RT-PCR to detect and type ovine pestiviruses", Journal of Virological Methods, vol.13, p.187-194, 2005/11/23 * |
| Xue W et al., "Vaccination with a modified-live bovine viral diarrhea virus (BVDV) type 1a vaccine completely protected calves against challenge with BVDV type 1b strains", Vaccine, vol.29, p.70-76, 2010/10/27 * |
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| Publication number | Publication date |
|---|---|
| PL2659007T3 (pl) | 2017-08-31 |
| JP6027023B2 (ja) | 2016-11-16 |
| CN103403191B (zh) | 2016-05-18 |
| ES2613537T3 (es) | 2017-05-24 |
| AU2011352801A1 (en) | 2013-06-27 |
| JP2014502846A (ja) | 2014-02-06 |
| MX348307B (es) | 2017-06-06 |
| US20130266934A1 (en) | 2013-10-10 |
| PT2659007T (pt) | 2017-01-18 |
| CA2822798C (en) | 2016-11-01 |
| EP2659007B1 (en) | 2016-11-16 |
| CN103403191A (zh) | 2013-11-20 |
| EP2659007A2 (en) | 2013-11-06 |
| MX2013007586A (es) | 2013-10-17 |
| TW201307571A (zh) | 2013-02-16 |
| BR112013016467A2 (pt) | 2018-09-18 |
| CA2822798A1 (en) | 2012-07-05 |
| AU2011352801B2 (en) | 2015-05-21 |
| NZ611657A (en) | 2015-04-24 |
| US9238843B2 (en) | 2016-01-19 |
| WO2012092054A3 (en) | 2012-12-27 |
| WO2012092054A2 (en) | 2012-07-05 |
| AR084358A1 (es) | 2013-05-08 |
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