ES2613537T3 - Procedimientos para identificar y diferenciar componentes víricos de vacunas multivalentes contra la fiebre del transporte - Google Patents

Procedimientos para identificar y diferenciar componentes víricos de vacunas multivalentes contra la fiebre del transporte Download PDF

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Publication number
ES2613537T3
ES2613537T3 ES11813620.9T ES11813620T ES2613537T3 ES 2613537 T3 ES2613537 T3 ES 2613537T3 ES 11813620 T ES11813620 T ES 11813620T ES 2613537 T3 ES2613537 T3 ES 2613537T3
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virus
specific
bvdv1b
amplification primer
nucleotides
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ES11813620.9T
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English (en)
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Dale Wade WEISE
James Robert HARRIS
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Eli Lilly and Co
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Eli Lilly and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Un procedimiento para detectar la presencia de un virus BVDV1b (virus de la diarrea vírica bovina de tipo 1b) vivo, en el que el procedimiento comprende detectar un polinucleótido específico de BVDV1b en una población de polinucleótidos obtenida de una pluralidad de partículas de virus contenidas en una muestra mediante las siguientes etapas: (A) Diluir la muestra en (i) una placa de ensayo que contenga células bovinas cultivadas, en la que las partículas de virus puedan replicarse y (ii) una placa de referencia sin células; (B) Realizar al menos una etapa de ciclado de una reacción en cadena de polimerasa cuantitativa en tiempo real en la placa de ensayo y en la placa de referencia, usando una composición que comprenda un primer cebador de amplificación oligonucleotídico específico de virus de menos de 50 nucleótidos de longitud que comprenda una secuencia de nucleótidos de SEQ ID NO: 1, un segundo cebador de amplificación oligonucleotídico específico de virus de menos de 50 nucleótidos de longitud que comprenda una secuencia de nucleótidos de SEQ ID NO: 2 y al menos una primera sonda de detección marcada, específica de virus, de menos de 50 nucleótidos de longitud que comprenda una secuencia de nucleótidos marcada de SEQ ID NO: 3, en el que el primer cebador de amplificación y el segundo cebador de amplificación producen un producto de amplificación específico de virus, en el que el producto de amplificación así producido se pone en contacto con al menos la primera sonda de detección marcada, en condiciones eficaces para detectar específicamente el producto de hibridación resultante, y en el que la detección del producto de hibridación resultante en la placa de ensayo, a un nivel más alto en comparación con el de la placa de referencia, indica la presencia del polinucleótido específico de BVDV1b de un virus BVDV1b vivo.

Description

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Claims (1)

  1. imagen1
ES11813620.9T 2010-12-27 2011-12-21 Procedimientos para identificar y diferenciar componentes víricos de vacunas multivalentes contra la fiebre del transporte Active ES2613537T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201061427404P 2010-12-27 2010-12-27
US201061427404P 2010-12-27
PCT/US2011/066392 WO2012092054A2 (en) 2010-12-27 2011-12-21 Compositions and methods for identifying and differentiating viral components of multivalent shipping fever vaccines

Publications (1)

Publication Number Publication Date
ES2613537T3 true ES2613537T3 (es) 2017-05-24

Family

ID=45532015

Family Applications (1)

Application Number Title Priority Date Filing Date
ES11813620.9T Active ES2613537T3 (es) 2010-12-27 2011-12-21 Procedimientos para identificar y diferenciar componentes víricos de vacunas multivalentes contra la fiebre del transporte

Country Status (15)

Country Link
US (1) US9238843B2 (es)
EP (1) EP2659007B1 (es)
JP (1) JP6027023B2 (es)
CN (1) CN103403191B (es)
AR (1) AR084358A1 (es)
AU (1) AU2011352801B2 (es)
BR (1) BR112013016467A2 (es)
CA (1) CA2822798C (es)
ES (1) ES2613537T3 (es)
MX (1) MX348307B (es)
NZ (1) NZ611657A (es)
PL (1) PL2659007T3 (es)
PT (1) PT2659007T (es)
TW (1) TWI513821B (es)
WO (1) WO2012092054A2 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019514429A (ja) * 2016-05-06 2019-06-06 プロビナンス バイオファブリックス インコーポレイテッド 培養レザー及びこれを用いて作製された製品
CN106906307A (zh) * 2017-03-10 2017-06-30 中国农业科学院特产研究所 牛副流感病毒3型病毒nano‑PCR检测试剂盒及其制备方法
CN113913461A (zh) * 2021-11-15 2022-01-11 贵州大学 牛病毒性腹泻e0-e2基因重组腺病毒疫苗构建方法

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FR2596761B1 (fr) 1986-04-08 1988-05-20 Commissariat Energie Atomique Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides
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Also Published As

Publication number Publication date
US9238843B2 (en) 2016-01-19
MX348307B (es) 2017-06-06
CN103403191B (zh) 2016-05-18
AU2011352801A1 (en) 2013-06-27
BR112013016467A2 (pt) 2018-09-18
MX2013007586A (es) 2013-10-17
CN103403191A (zh) 2013-11-20
WO2012092054A2 (en) 2012-07-05
CA2822798C (en) 2016-11-01
PL2659007T3 (pl) 2017-08-31
EP2659007B1 (en) 2016-11-16
AU2011352801B2 (en) 2015-05-21
JP2014502846A (ja) 2014-02-06
NZ611657A (en) 2015-04-24
WO2012092054A3 (en) 2012-12-27
PT2659007T (pt) 2017-01-18
US20130266934A1 (en) 2013-10-10
TWI513821B (zh) 2015-12-21
CA2822798A1 (en) 2012-07-05
AR084358A1 (es) 2013-05-08
JP6027023B2 (ja) 2016-11-16
TW201307571A (zh) 2013-02-16
EP2659007A2 (en) 2013-11-06

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