TW558638B - Diagnostic based on tetrazolium compounds - Google Patents
Diagnostic based on tetrazolium compounds Download PDFInfo
- Publication number
- TW558638B TW558638B TW088106574A TW88106574A TW558638B TW 558638 B TW558638 B TW 558638B TW 088106574 A TW088106574 A TW 088106574A TW 88106574 A TW88106574 A TW 88106574A TW 558638 B TW558638 B TW 558638B
- Authority
- TW
- Taiwan
- Prior art keywords
- analyte
- patent application
- enzyme
- item
- scope
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/525—Multi-layer analytical elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/64—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving ketones
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/97—Test strip or test slide
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/17—Nitrogen containing
- Y10T436/173076—Nitrite or nitrate
Description
558638 A7 B7 五、發明説明(> ) 經濟部智慧財產局員工消費合作社印製 吕,β-經丁S欠鹽、乙酿乙酸鹽及丙g同的百分比分別為% %、20%及2%,由於丙酮有相當低的濃度及高揮發性 因此很少偵測,替代地,經由硝普鹽半胱胺酸呈色反應定 量偵測乙醯乙酸鹽,並經由酵素法定量心羥丁酸鹽,乙醯 乙酸鹽測试條已經供應數十年,其係建立在石肖普鹽離子與 醛及酮之偶合反應,使鹼性尿液樣本或血清樣本與硝普鹽 反應數刀4里,並舍展成务、色,顏色的強度顯示乙酿乙酸鹽 之濃度,但是丙酮會干擾此測試而導致讀值偏高,而且當 病人從酮酸中毒事件恢復時,尿液及血液中的乙醯乙酸鹽 含量增加,因此使得診斷很困難。 β-羥丁酸鹽測試更有用於監測酮體濃度,其係建立在 β -經丁酸鹽與相對應的脫氫酶在於驗酸酿胺腺嗓呤二核苷 酸(NAD)輔因子存在下之氧化反應(嚴袼說來,只有〇_0_羥 丁酸鹽是自然存在及氧化,但是我們為了簡化起見,在本 說明書及附帶的專利申請範圍中省略”D,,),當氧化時,產 生NADH,其濃度可用UV光度計直接測量,因此光譜中相 對應訊號的變化與分析物的濃度成正比,不巧的是, NADH的激發是發生在UV範圍,所以此偵測模式只適合實 驗室儀器,監測β-經丁酸鹽的另一種方法是用四銼化合物 氧化NADH。 四經化合物通常對強鹼及光非常敏感,因此必須特別 小心以破保這些化合物之原樣,但是四銼化合物在研究組 織代謝中扮演重要的角色,例如此種化合物曾用於在細胞 中探測厭氧性氧化及還原反應,而且普遍用在臨床診斷, 〜4〜 本紙張尺度適用中國國家標準(CNS ) Α4規格(21〇χ297公董) (請先閱讀背面之注意事項再填寫本頁) 訂_ 558638 A7 _B7 五、發明説明(3 ) 此種化合物通常是淺色或無色的化合物,在還原劑存在下 進行還原反應而產生深色的甲替,例如抗壞血酸、硫氫化 物或不同的NADH及NADPH之還原劑可形成染色。 在臨床診斷中,發現這些染色對於在厭氧性反應中監 測從母化合物NAD(P)+形成NAD(P)H沒有用處,此氧化還 原反應快速且對氧不敏感,所得的染料顏色非常強烈且在 水中的溶解度低。 原則上,四銼染料前驅物可用於測量全血中的g同體及 葡萄糖,但是如果血紅素不是包含在血液的紅血球内,四 銼化合物可被血紅素(Fe(II))非酵素性還原而形成有顏色的 甲月替,因此,自由態的血紅素在測量時造成嚴重的干擾, 事實上,由於血球溶解及所得的相對於所要分析物質之大 量自由態的血紅素,在典型的臨床樣本中,從血紅素之干 擾訊號將超越所要的訊號,此點尤其真實於高血球比容樣 品或當在較高的溫度下進行反應時,此時血紅素氧化反應 較快速,因為無法輕易地避免紅血球細胞之血球溶解而造 成自由態的血紅素存在,如果使用四銼化合物分析時,在 測试刖必須先將紅血球細胞去除。 用膜或濾紙過濾,經由化學藥劑捕集,或兩種方法之 結合,可將紅血球細胞從樣品中去除,從全血分離紅血球 細胞的過濾法昂貴且需要相當大的樣品體積,使用過遽法 從全血樣品中去除紅血球細胞之血酮(β-羥丁酸鹽)測試實 例是可得自 GDS Diagnostics,Elkhart,IN之KetoSite、#&(見 Tietz Textbook of Clinical Chemistry,2nd Ed.,ed. By C. Burtis 〜, 本紙張尺度適用中國國家標準(CNS ) A4規格(21〇 Χ:297公釐) ----------— (請先閲讀背面之注意事項再填寫本頁) 訂 4 經濟部智慧財產局員工消費合作社印製 558638 A7 ---— --—-_________ 五、發明説明(β) et al.,W· Β· Saunders Co” Philadelphia,PA,1994, ρ· 974), 在該測試中使用的”測試卡,,含有兩個濾紙層,使得該卡相 當昂貴且需要大量(25微升)的血液樣品,而且血液必須不 能被血球溶解化。 過濾及化學捕集的組合用在可得自 Glucometer Encore™血液葡萄糖長條物,該長條物使用一 層濾紙物質及一種凝集助劑(馬龄薯卵構脂)以消除紅血球 細胞之干擾(見Chu et al·,1995年2月15日公告之歐洲專利申 請案0 638 805 A2)。 在系統中加入一種氧化劑,將血紅素氧化成高 鐵血紅 蛋白,是減低血紅素干擾的另一種方法,雖然已知鐵氰化 物可將血紅素轉化成高鐵血紅蛋白,其也會破壞所要的產 物NADH。 發明概述 本發明提供一種試劑供在含血紅素的生物體液中測量 分析物濃度,該試劑含: a) 對分析物具有專一性之脫氫酶酵素, b) 菸鹼酸醯胺腺嘌呤二核苷酸(ΝΑ〇)4ΝΑΕ^ί生物, c) 四銼染料前驅物, ’ d) 心肌黃酶酵素或其類似物,及 e) 亞硝酸鹽。 、孩5式劑特別合適供塗覆在一或多種基質上以形成乾的 试劑長條物供測量分析物,特別較佳的長條物包含 a)支撐層, 〜6〜 本紙張尺錢财_^i?TcNS )7¾ ( 210X297^) ---------_衣 II (請先閱讀背面之注意事項再填寫本頁) 訂 # 經濟部智慧財產局員工消費合作社印製 合適物質,峨麵*上塗覆與分析物反躺造脑色變化 之試劑,墊較宜含魏性的物f,例如濾紙或聚合 物膜,但是因為反應不需要氧,測試襯塾可為非吸收性的 物貝例如塑膠膜,試劑含對分析物具有專一性的酵素、氮 轉移劑、四轉料前驅物、合適的酵素綱子、及血紅素 抑制劑,視需要可選用緩衝劑或安定劑供較大的安定性。 如圖2所示,測試長條物也可為多層結構,以頂層16 覆蓋測試襯墊12,在該結構中,試劑可分散在兩層之間, 例如血=素抑制劑可塗覆在視需要選用的頂層16上,並將 八餘的办彳塗覆在測試襯墊12上,較宜頂層%具有吸收性 且作為分散劑及吸收層以吸附過量的樣品,將樣品添加至 頂層16,且通過至測試襯墊12,經由測量通過支撐層μ或 當連接反舰域之層14不透明時,通過視需要的窗口 或中空洞18之顏色變化而測定分析物之濃度。 在圖3顯示的另一個具體實施例中,間隔層20將頂層 16及測5式襯塾12分離,間隔層2〇較宜為非吸收性的塑膠 膜,在兩面都有黏著性塗層(沒有顯示),間隔層20中的通 ,22提供毛細管通道,供樣品從開口 24流至測試區域26, 流動決定於測試襯墊12的表面與連接層之間的空氣排氣, 或者是經由視需要選用的排氣口 18,測量區域26的顏色變 化:二由視品要選用的棑氣口/窗口 18監視,試劑可全部在測 試襯塾12或者是分散在測試襯墊及—或同時兩個非吸收性 層14及16,當我們稱試劑,,塗層,,或在塗層,,上面,,時,我們 558638 五、發明説明( 經濟部智慧財產局員工消費合作社印製 是指包括觸吸附至塗層_可能性,尤其是如果其具有 吸收性。 適合供本發明測試法的酵素及相對應的分析物為:對 3的醇脫氫酶、對於甲_甲魏氳酶、對於葡萄糖的 々萄糖脫氫酶、對於葡萄糖_6_碌酸鹽的葡萄糖―心磷酸鹽脫 氧酶、對於榖胺酸的榖胺酸脫氫酶、對於甘油的甘油脫氫 鴎、對於点-羥丁酸鹽的榖石-羥丁酸鹽脫氫酶、對於類固 醇的羥類固醇脫氫酶、對於L-乳酸鹽的L-乳酸鹽脫氫酶、 對於冗胺酸的焭胺酸脫氫酶、對於蘋果酸的蘋果酸脫氫 酶、及對於丙酮酸的丙酮酸脫氫酶。 需要合適的酵素輔因子以活化酵素,決定於酵素,這 些輔因子可使用:石_終驗酸醯胺腺嘌呤二核苷酸(召_ NAD)、/9-菸鹼酸醯胺腺嘌呤二核苷酸碌酸(万_NADp)、 硫代菸鹼酸醯胺腺嘌呤二核苷酸、硫代菸鹼酸醯胺腺嘌呤 二核苷酸碌酸、菸鹼酸醯胺1,N6-乙烯腺嘌呤二核苷酸及 菸鹼酸醯胺1,N6-乙烯腺嘌呤二核苷酸磷酸,在酵素存在 下,分析物可還原輔因子。 在染色形成過程的下一步驟是從還原的輔因子提取氫 化物,其可經由心肌黃酶例如硫辛酸脫氫酶、鐵氧化還原 蛋白-NADP還原酶、硫辛醯胺脫氫酶、或經由合成類似物 例如吩嗪甲基硫酸鹽(PMS)或Meldola Blue完成,反 應動力學及安定性為選擇氫化物轉移劑或”提取劑”之 主要因素,例如,PMS為廣用的氫化物提取劑,因為 其對於大部分下列四經化合物具有相當快速的反應動 〜9〜 本紙張尺度適用中國國家標準(CNS ) A4規格(210x297公釐) I--------II (請先閱讀背面之注意事項再填寫本頁) 訂- 經濟部智慧財產局員工消費合作社印製 558638 A7 B7 五、發明説明(f) 力,但是PMS對光的敏感性高於以酵素為主的氫化物 提取劑,心肌黃酶較安定,且基於此理由而較佳。 被捕集的氫化物轉移至四銼化合物(染料前驅物) 而形成有色的甲檢,此裝置最合適的四銼化合物為: 2-(2-苯並嗟唾基)-:5-苯乙晞基-3-(4’-g太耕基)四銼 (BSPT)、2-苯並噻唑基-(2)-3,5_二苯基四銼(BTDP)、 2,3-二(4-硝基苯基)四銼(DNP)、2,5-二苯基_3_(苯乙 烯苯基)四銼(DPSP)、二苯乙烯基硝基藍四銼⑴S- NBT)、 3,3-[3,3’-二 甲氧基-(1,1_聯苯基 )_4,4,_二基]_ 雙[2-(4_硝基苯基)-5-苯基]_2H-四銼(NBT)、3_(4,5-5 二甲基_2_噻唑基)-2,5-二苯基·2Η四銼(MTT)、2_苯基 -3-(4-竣苯基)-5·甲基四銼(PCPM)、四銼藍(ΤΒ)、硫 代胺基甲醯基硝基藍四銼(TCNBT)、四硝基藍四銼 (TNBT)、四銼紫(丁V)、2 -苯並σ塞唾π塞ϋ坐基_3-(4_緩基_2· 甲氧基苯基)-Η4_(2-硫代乙基胺基甲醯基)苯基]_2HL四銼 (WST-4)及2,2’-二苯並噻唑基_5,5,_雙[4_二(2·硫代乙基)胺基 甲醯基苯基]_3,3,_(3,3,-二甲氧基-4,4,_二伸絲)三四銼二納 鹽(WST-5),以WST-5較佳,因為其在水性介質中容易分 解,與生物樣品最相容,而且,所得的甲肖替化合物在紫_ 藍區域顯示強烈的吸收光譜,因此減少從血紅素校正背景 訊號之需求。 最後,在試劑中存在血紅素抑制劑可減少血紅素與四 f化口物之間不欲的染色形成反應,血紅素抑制劑的角色 疋將血紅素氧化成高鐵錄蛋白,其不會與四銼反應,舒 ------------ (請先閱讀背面之注意事項再填寫本頁)
、1T • ί I— 〜1〇〜
558638 A7 B7 五、發明説明(// ) 經濟部智慧財產局員工消費合作社印製 表1.酮體測試襯墊之試劑 成份 量 水 100毫升 參(羥甲基)胺基甲烷(MW 121,Sigma,St. Louis, 1·2克 M〇,USA)(經由加入6莫耳濃度HC1將pH調整成 8.5) 氯化鈉(MW 56.44, Sigma,St. Louis, MO, USA) 560毫克 氯化鎂(MW 203, Sigma,St. Louis, MO, USA) 2.5克 PSSA,聚苯乙烯磺酸鈉鹽(MW70,000, 3克 Polysciences, Inc.5 Warrington, PA? USA) Crotein (Croda Inc. Parsippany,NJ,USA) 3克 草胺酸納鹽(MW 111.03, Aldrich Chemicals, 250毫克 Milwaukee, WI, USA) Tetronic 1307 (BASF Corporation, Mount Olive, 2克 New Jersey, USA) 蔗糖(MW 342.30, Aldrich Chemicals,Milwaukee, 5克 WI? USA) _____一 NAD (MW 663.4, N-7004, Sigma,St· Louis,MO, 450毫克 USA) D-3-經丁酸鹽脫氫酶(來源:Pseudomonas sp·, 5〇,〇〇° υ HBD-301,125 U/毫克,Toyobo, Japan) 340890^ 心肌黃酶(來源:B. Stearothermophilus,New, 1033 U/毫克,Toyobo, Japan) WST-5 (MW 1331.37, Dojindo, Japan) ---------4—, (請先閱讀背面之注意事項再填寫本頁) 命 〜13〜 本紙張尺度適用中國國家標準(CNS ) A4規格(210X297公釐) 558638 A7 B7 五、發明説明(/>) 經濟部智慧財產局員工消費合作社印製 表2.亞硝酸鹽試劑 成份 量 10毫莫耳濃度磷酸鹽緩衝鹽水,pH 7.4 (P_3813, Sigma,St· Louis,M〇,USA) 70毫升 乙醇 30毫升 亞石肖酸納(MW 69, Aldrich Chemicals, Milwaukee, WI,USA) 5克 聚乙稀基。比洛^(MW 40,000, Sigma,St. Louis, M〇,USA) 200亳克 草胺酸鈉鹽(MW 111.03, Aldrich Chemicals, Milwaukee, WI5 USA) 500毫克 (請先閲讀背面之注意事項再填寫本頁) 本紙張尺度適用中國國家標準(CNS ) A4規格(210X297公釐)
Claims (1)
- 558638 公告本、申請專利範圍 A8 B8 C8 D8 種4劑,供在含血紅素的生物體液中測量分析物濃度 ’其中含: & a) 對分析物具有專一性之脫氫酶酵素, b) 菸鹼酸醯胺腺嘌呤二核苷酸_))或_衍生物, c) 四銼染料前驅物, ’ Φ心肌黃酶酵素或其類似物,及 匀亞硝酸鹽。 2·根據申請專利範圍第1項之試劑,其中該分析物為卜經 丁酸鹽且酵素為β_羥丁酸鹽脫氫酶。 3. —種乾的试劑長條物,供在含血紅素的生物體液中測量 分析物的存在及量,其中含一個支撐層,該層上面含根據 申請專利範圍第1項的試劑塗覆之測試襯墊。 4. 根據申凊專利範圍第3項之長條物,其中還含吸收性頂 層覆蓋之測試墊。 ' 5·—種乾的試劑長條物,供在含血紅素的生物體液中測量 分析物的存在及量,其中含一個支撐層,該層上面為測試 襯墊,在測試襯墊的上面覆蓋頂層,其中根據申請專利範 圍第1項的試劑之第一個部份是在測試襯墊上,且試劑之 第二個部份是在支撐層及/或頂層上。 6. 根據申請專利範圍第5項之長條物,其中頂層具有吸收 性。 7. 根據申請專利範圍第5項的長條物,在頂層與測試襯塾 之間還含間隔層及通道,以提供頂層與襯墊之間的毛細管 通道。 〜15〜 本紙張尺度適财目國家麟(CNS)A4· (21GX297公楚) ------- (請先閲讀背面之注意事項再填寫本頁) 、11 經濟部智慧財產局員工消費合作社印製 558638 A8 B8 C8 D8 六、申請專利範圍 經濟部智慧財產局員工消費合作社印製 8. 根據申請專利範圍第5項的長條物,其中該分析物 羥丁酸鹽且酵素為β_羥丁酸鹽脫氫酶。 9. 根據申請專利範圍第5項的長條物,其中該分析物 萄糖且酵素為葡萄糖脫氫酶。 10·根據申請專利範圍第5項的長條物,其中分析物為葡萄 糖-6-碟酸鹽且酵素為葡萄糖_6_鱗酸鹽脫氫酶。 11.根據申晴專利範圍第5項的長條物,其中分析物為醇且 酵素為醇脫氫酶。 12·根據申請專利範圍第5項的長條物,其中分析物為1_乳 酸鹽且酵素為L·乳酸鹽脫氫酶。 13. 根據申請專利範圍第5項的長條物,其中四銼染料前驅 物為2,2’-二笨並噻唑基_5,5,_雙[4_二(2_硫代乙基)胺基甲醯 基苯基]_3,3’_(3,3’-二甲氧基_4,4,-二伸苯基)二四銼二鈉鹽 (WST_5)。 孤 14. 一種乾的试劑測試長條物,供在含血紅素的生物體液中 測置分析物的存在及量,其中含 a) 支撐層, b) 在支撐層上含有塗覆物的測試襯墊,該塗覆物含 i) 對分析物具有專一性之脫氫酶酵素, ii) 菸鹼酸醯胺腺嘌呤二核苷酸(NAD)或NAD衍 生物, iii) 四銼染料前驅物,及 iv) 心肌黃酶酵素或其類似物, 及 Λ6- 家標準(CNS ) A4規格(210X297公釐) 41^— (請先閱讀背面之注意事項再填寫本頁) 訂 558638 A8 B8 C8 D8 申請專利範圍 C)在測試襯墊上,塗覆亞硝酸鹽之吸收性頂層 (請先閱讀背面之注意事項再填寫本頁) 經濟部智慧財產局員工消費合作社印製 本紙張尺度適用中國國家標準(CNS ) A4規格(210X297公釐)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/161,876 US5902731A (en) | 1998-09-28 | 1998-09-28 | Diagnostics based on tetrazolium compounds |
Publications (1)
Publication Number | Publication Date |
---|---|
TW558638B true TW558638B (en) | 2003-10-21 |
Family
ID=22583150
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TW088106574A TW558638B (en) | 1998-09-28 | 1999-04-23 | Diagnostic based on tetrazolium compounds |
TW088110056A TW594009B (en) | 1998-09-28 | 1999-06-16 | Diagnostics based on tetrazolium compounds |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TW088110056A TW594009B (en) | 1998-09-28 | 1999-06-16 | Diagnostics based on tetrazolium compounds |
Country Status (22)
Country | Link |
---|---|
US (2) | US5902731A (zh) |
EP (2) | EP0990705B1 (zh) |
JP (2) | JP2000093198A (zh) |
KR (2) | KR100570909B1 (zh) |
CN (2) | CN1155829C (zh) |
AR (2) | AR014718A1 (zh) |
AT (2) | ATE222961T1 (zh) |
AU (2) | AU754321B2 (zh) |
BR (2) | BR9901186A (zh) |
CA (2) | CA2265201A1 (zh) |
DE (2) | DE69902612T2 (zh) |
DK (2) | DK0990705T3 (zh) |
ES (2) | ES2183481T3 (zh) |
HK (1) | HK1026001A1 (zh) |
IL (2) | IL128905A (zh) |
MY (2) | MY117657A (zh) |
NO (2) | NO991185L (zh) |
PT (2) | PT990705E (zh) |
RU (2) | RU2225004C2 (zh) |
SG (1) | SG82610A1 (zh) |
TW (2) | TW558638B (zh) |
ZA (2) | ZA991906B (zh) |
Families Citing this family (129)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6335203B1 (en) * | 1994-09-08 | 2002-01-01 | Lifescan, Inc. | Optically readable strip for analyte detection having on-strip orientation index |
US8071384B2 (en) | 1997-12-22 | 2011-12-06 | Roche Diagnostics Operations, Inc. | Control and calibration solutions and methods for their use |
US6391005B1 (en) | 1998-03-30 | 2002-05-21 | Agilent Technologies, Inc. | Apparatus and method for penetration with shaft having a sensor for sensing penetration depth |
US6656697B1 (en) * | 1998-09-28 | 2003-12-02 | Lifescan, Inc. | Diagnostics based on tetrazolium compounds |
US5902731A (en) * | 1998-09-28 | 1999-05-11 | Lifescan, Inc. | Diagnostics based on tetrazolium compounds |
US20050103624A1 (en) * | 1999-10-04 | 2005-05-19 | Bhullar Raghbir S. | Biosensor and method of making |
PL357180A1 (en) * | 2000-03-28 | 2004-07-26 | Lifescan, Inc. | Reagent systems for detecting the presence of a reduced cofactor in a sample and methods for using the same |
US6420128B1 (en) * | 2000-09-12 | 2002-07-16 | Lifescan, Inc. | Test strips for detecting the presence of a reduced cofactor in a sample and method for using the same |
EP1329721B8 (en) * | 2000-09-28 | 2010-05-26 | ARKRAY, Inc. | Method of quantifying hemoglobin and method of measuring glycation ratio of hemoglobin |
US8641644B2 (en) | 2000-11-21 | 2014-02-04 | Sanofi-Aventis Deutschland Gmbh | Blood testing apparatus having a rotatable cartridge with multiple lancing elements and testing means |
US6891685B2 (en) * | 2001-05-17 | 2005-05-10 | Sioptical, Inc. | Anisotropic etching of optical components |
ES2336081T3 (es) | 2001-06-12 | 2010-04-08 | Pelikan Technologies Inc. | Dispositivo de puncion de auto-optimizacion con medios de adaptacion a variaciones temporales en las propiedades cutaneas. |
DE60238119D1 (de) | 2001-06-12 | 2010-12-09 | Pelikan Technologies Inc | Elektrisches betätigungselement für eine lanzette |
US7025774B2 (en) | 2001-06-12 | 2006-04-11 | Pelikan Technologies, Inc. | Tissue penetration device |
US9226699B2 (en) | 2002-04-19 | 2016-01-05 | Sanofi-Aventis Deutschland Gmbh | Body fluid sampling module with a continuous compression tissue interface surface |
US9795747B2 (en) | 2010-06-02 | 2017-10-24 | Sanofi-Aventis Deutschland Gmbh | Methods and apparatus for lancet actuation |
US7981056B2 (en) | 2002-04-19 | 2011-07-19 | Pelikan Technologies, Inc. | Methods and apparatus for lancet actuation |
US9427532B2 (en) | 2001-06-12 | 2016-08-30 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
WO2002100254A2 (en) | 2001-06-12 | 2002-12-19 | Pelikan Technologies, Inc. | Method and apparatus for lancet launching device integrated onto a blood-sampling cartridge |
US8337419B2 (en) | 2002-04-19 | 2012-12-25 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
DE60237463D1 (de) * | 2001-11-16 | 2010-10-07 | Roche Diagnostics Gmbh | Flexibler sensor und herstellungsverfahren |
US6586199B2 (en) * | 2001-11-20 | 2003-07-01 | Lifescan, Inc. | Stabilized tetrazolium reagent compositions and methods for using the same |
US6939685B2 (en) | 2001-11-20 | 2005-09-06 | Lifescan, Inc. | Stabilized tetrazolium phenazine reagent compositions and methods for using the same |
US6762035B1 (en) * | 2002-02-04 | 2004-07-13 | Surendra K. Gupta | Method and test strips for the measurement of fat loss during weight loss programs |
GB0204232D0 (en) * | 2002-02-22 | 2002-04-10 | Isis Innovation | Assay |
US6703216B2 (en) | 2002-03-14 | 2004-03-09 | The Regents Of The University Of California | Methods, compositions and apparatuses for detection of gamma-hydroxybutyric acid (GHB) |
WO2003079890A1 (en) * | 2002-03-26 | 2003-10-02 | Piet Moerman | A method and apparatus for quantifying caloric balance using metabolic parameters to assist subjects on weight management |
US7547287B2 (en) | 2002-04-19 | 2009-06-16 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US8221334B2 (en) | 2002-04-19 | 2012-07-17 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
US8372016B2 (en) | 2002-04-19 | 2013-02-12 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for body fluid sampling and analyte sensing |
US7892183B2 (en) | 2002-04-19 | 2011-02-22 | Pelikan Technologies, Inc. | Method and apparatus for body fluid sampling and analyte sensing |
US7674232B2 (en) | 2002-04-19 | 2010-03-09 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7976476B2 (en) | 2002-04-19 | 2011-07-12 | Pelikan Technologies, Inc. | Device and method for variable speed lancet |
US7708701B2 (en) | 2002-04-19 | 2010-05-04 | Pelikan Technologies, Inc. | Method and apparatus for a multi-use body fluid sampling device |
US7232451B2 (en) | 2002-04-19 | 2007-06-19 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US9248267B2 (en) | 2002-04-19 | 2016-02-02 | Sanofi-Aventis Deustchland Gmbh | Tissue penetration device |
US7331931B2 (en) | 2002-04-19 | 2008-02-19 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US8267870B2 (en) | 2002-04-19 | 2012-09-18 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for body fluid sampling with hybrid actuation |
US7229458B2 (en) | 2002-04-19 | 2007-06-12 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7901362B2 (en) | 2002-04-19 | 2011-03-08 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7297122B2 (en) | 2002-04-19 | 2007-11-20 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US9314194B2 (en) | 2002-04-19 | 2016-04-19 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
US8360992B2 (en) | 2002-04-19 | 2013-01-29 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
US7909778B2 (en) | 2002-04-19 | 2011-03-22 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US8702624B2 (en) | 2006-09-29 | 2014-04-22 | Sanofi-Aventis Deutschland Gmbh | Analyte measurement device with a single shot actuator |
US8784335B2 (en) | 2002-04-19 | 2014-07-22 | Sanofi-Aventis Deutschland Gmbh | Body fluid sampling device with a capacitive sensor |
US7175642B2 (en) | 2002-04-19 | 2007-02-13 | Pelikan Technologies, Inc. | Methods and apparatus for lancet actuation |
US8579831B2 (en) | 2002-04-19 | 2013-11-12 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
US9795334B2 (en) | 2002-04-19 | 2017-10-24 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
US7491178B2 (en) | 2002-04-19 | 2009-02-17 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
ATE420367T1 (de) * | 2002-06-07 | 2009-01-15 | Arkray Inc | Testverfahren mit einer oxidations- /reduktionsreaktion mit formazan |
US7354732B2 (en) * | 2002-06-14 | 2008-04-08 | Arkray, Inc. | Method of assay with sulfonic acid compound and nitro compound |
JP3973160B2 (ja) * | 2002-07-17 | 2007-09-12 | アークレイ株式会社 | スルホン酸化合物を用いたタンパク質の分解方法 |
US8574895B2 (en) | 2002-12-30 | 2013-11-05 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus using optical techniques to measure analyte levels |
US20040148942A1 (en) * | 2003-01-31 | 2004-08-05 | Capstone Turbine Corporation | Method for catalytic combustion in a gas- turbine engine, and applications thereof |
CN1756834B (zh) * | 2003-02-24 | 2010-05-26 | 比南股份有限公司 | 干式化学、侧向流动重建流的层析酶驱动测定 |
US20040241779A1 (en) * | 2003-02-24 | 2004-12-02 | Piasio Roger N. | Dry chemistry, lateral flow-reconstituted chromatographic enzyme-driven assays |
US20040214345A1 (en) * | 2003-04-23 | 2004-10-28 | Matzinger David P. | Ambidextrous capillary-filled test strip |
US20040219691A1 (en) * | 2003-04-29 | 2004-11-04 | Shartle Robert J. | Test strip with clear base support layer for visual perception of a liquid sample during application |
ATE476137T1 (de) | 2003-05-30 | 2010-08-15 | Pelikan Technologies Inc | Verfahren und vorrichtung zur injektion von flüssigkeit |
DK1633235T3 (da) | 2003-06-06 | 2014-08-18 | Sanofi Aventis Deutschland | Apparat til udtagelse af legemsvæskeprøver og detektering af analyt |
WO2006001797A1 (en) | 2004-06-14 | 2006-01-05 | Pelikan Technologies, Inc. | Low pain penetrating |
US7645373B2 (en) | 2003-06-20 | 2010-01-12 | Roche Diagnostic Operations, Inc. | System and method for coding information on a biosensor test strip |
CA2529657C (en) | 2003-06-20 | 2011-04-12 | F. Hoffmann-La Roche Ag | Test strip with slot vent opening |
US8148164B2 (en) | 2003-06-20 | 2012-04-03 | Roche Diagnostics Operations, Inc. | System and method for determining the concentration of an analyte in a sample fluid |
US8206565B2 (en) | 2003-06-20 | 2012-06-26 | Roche Diagnostics Operation, Inc. | System and method for coding information on a biosensor test strip |
US8058077B2 (en) | 2003-06-20 | 2011-11-15 | Roche Diagnostics Operations, Inc. | Method for coding information on a biosensor test strip |
US7718439B2 (en) | 2003-06-20 | 2010-05-18 | Roche Diagnostics Operations, Inc. | System and method for coding information on a biosensor test strip |
US7452457B2 (en) | 2003-06-20 | 2008-11-18 | Roche Diagnostics Operations, Inc. | System and method for analyte measurement using dose sufficiency electrodes |
US8071030B2 (en) * | 2003-06-20 | 2011-12-06 | Roche Diagnostics Operations, Inc. | Test strip with flared sample receiving chamber |
US8679853B2 (en) * | 2003-06-20 | 2014-03-25 | Roche Diagnostics Operations, Inc. | Biosensor with laser-sealed capillary space and method of making |
US7488601B2 (en) | 2003-06-20 | 2009-02-10 | Roche Diagnostic Operations, Inc. | System and method for determining an abused sensor during analyte measurement |
US7645421B2 (en) | 2003-06-20 | 2010-01-12 | Roche Diagnostics Operations, Inc. | System and method for coding information on a biosensor test strip |
EP1671096A4 (en) | 2003-09-29 | 2009-09-16 | Pelikan Technologies Inc | METHOD AND APPARATUS FOR PROVIDING IMPROVED SAMPLE CAPTURING DEVICE |
EP1680014A4 (en) | 2003-10-14 | 2009-01-21 | Pelikan Technologies Inc | METHOD AND APPARATUS PROVIDING A VARIABLE USER INTERFACE |
US7822454B1 (en) | 2005-01-03 | 2010-10-26 | Pelikan Technologies, Inc. | Fluid sampling device with improved analyte detecting member configuration |
EP1706026B1 (en) | 2003-12-31 | 2017-03-01 | Sanofi-Aventis Deutschland GmbH | Method and apparatus for improving fluidic flow and sample capture |
RU2006132051A (ru) | 2004-02-06 | 2008-03-20 | БАЙЕР ХЕЛТКЭР ЭлЭлСи (US) | Окисляемые соединения в качестве внутреннего стандарта для биосенсоров и способ их применения |
JP2005257354A (ja) * | 2004-03-10 | 2005-09-22 | Fuji Photo Film Co Ltd | 一体型マイクロケミカルデバイス |
US8828203B2 (en) | 2004-05-20 | 2014-09-09 | Sanofi-Aventis Deutschland Gmbh | Printable hydrogels for biosensors |
US9775553B2 (en) | 2004-06-03 | 2017-10-03 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for a fluid sampling device |
WO2005120365A1 (en) | 2004-06-03 | 2005-12-22 | Pelikan Technologies, Inc. | Method and apparatus for a fluid sampling device |
US7569126B2 (en) | 2004-06-18 | 2009-08-04 | Roche Diagnostics Operations, Inc. | System and method for quality assurance of a biosensor test strip |
US20060024835A1 (en) | 2004-07-30 | 2006-02-02 | Matzinger David P | Analytical test strip with control zone |
US8080423B2 (en) * | 2004-08-05 | 2011-12-20 | Asahi Kasei Pharma Corporation | Reagent containing protease reaction promoter and/or colorant stabilizer |
WO2006023927A1 (en) * | 2004-08-24 | 2006-03-02 | Bayer Healthcare Llc | Method for determining the concentration of analytes in samples by direct mediation of enzymes |
CA2590944C (en) | 2004-12-13 | 2016-02-02 | Bayer Healthcare Llc | Size self-limiting compositions and test devices for measuring analytes in biological fluids |
WO2006064488A1 (en) * | 2004-12-17 | 2006-06-22 | Megazyme Ip Limited | A kit for colorimetric assays of food and beverage analytes |
US8652831B2 (en) | 2004-12-30 | 2014-02-18 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for analyte measurement test time |
TW200642655A (en) * | 2005-02-01 | 2006-12-16 | Bayer Healthcare Llc | Sensor and package |
EP1741869A1 (en) | 2005-07-08 | 2007-01-10 | Cuhadaroglu Metal Sanayi Ve Pazarlama A.S. | Doors, windows, curtain walls composed of high thermal insulated, wooden-aluminium composite profiles, and production method thereof |
AR054851A1 (es) | 2005-07-20 | 2007-07-18 | Bayer Healthcare Llc | Amperometria regulada |
US8404100B2 (en) | 2005-09-30 | 2013-03-26 | Bayer Healthcare Llc | Gated voltammetry |
DE102006002165A1 (de) * | 2006-01-17 | 2007-07-19 | Merck Patent Gmbh | Verfahren und Mittel zur enzymatischen Bestimmung von Acetat |
AU2007207242B2 (en) | 2006-01-19 | 2012-08-02 | Lattec I/S | Dry stick device and method for determining an analyte in a sample |
AU2007207241B2 (en) | 2006-01-19 | 2012-07-26 | Lattec I/S | A novel dry stick device construction and method for determining an analyte in a sample using said dry stick device |
US7531319B2 (en) * | 2006-08-31 | 2009-05-12 | Kimberly-Clark Worldwide, Inc. | Array for rapid detection of a microorganism |
CN1996009B (zh) | 2007-01-10 | 2010-05-19 | 博奥生物有限公司 | 一种用于多样品分析的微流体器件和使用方法 |
JP4697809B2 (ja) * | 2007-02-22 | 2011-06-08 | 旭化成ファーマ株式会社 | ロイコ色素の安定化方法 |
US20080297169A1 (en) * | 2007-05-31 | 2008-12-04 | Greenquist Alfred C | Particle Fraction Determination of A Sample |
WO2009076302A1 (en) | 2007-12-10 | 2009-06-18 | Bayer Healthcare Llc | Control markers for auto-detection of control solution and methods of use |
US20090219509A1 (en) * | 2008-02-29 | 2009-09-03 | Hiroshi Nomura | Optical sensor with enhanced reflectance |
US8008068B2 (en) * | 2008-02-29 | 2011-08-30 | Light Pointe Medical, Inc. | Nonhemolytic optical sensor with enhanced reflectance |
US8008037B2 (en) | 2008-03-27 | 2011-08-30 | Roche Diagnostics Operations, Inc. | Matrix composition with alkylphenazine quaternary salt and a nitrosoaniline |
WO2009126900A1 (en) | 2008-04-11 | 2009-10-15 | Pelikan Technologies, Inc. | Method and apparatus for analyte detecting device |
EP2295969B1 (en) * | 2008-07-04 | 2016-05-18 | Sekisui Medical Co., Ltd. | Method for enhancing sensitivity or method for avoiding influence of hemoglobin in immunological measurement |
US9375169B2 (en) | 2009-01-30 | 2016-06-28 | Sanofi-Aventis Deutschland Gmbh | Cam drive for managing disposable penetrating member actions with a single motor and motor and control system |
AU2009347569A1 (en) * | 2009-06-08 | 2011-12-15 | Protea Biopharma N.V. | Methods and kits for detecting, diagnosing and monitoring diseases |
CN101865853B (zh) * | 2010-03-16 | 2012-06-27 | 苏州市玮琪生物科技有限公司 | 一种稳定化的β-羟丁酸检测试纸及其制备方法 |
US8965476B2 (en) | 2010-04-16 | 2015-02-24 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
CN103842523A (zh) * | 2011-07-22 | 2014-06-04 | 阿赛斯生物股份有限公司 | 用于改善的侧向流动测定法的单一垫条及使用其的测试装置 |
CN102520198A (zh) * | 2011-11-21 | 2012-06-27 | 宁波美康生物科技股份有限公司 | 乙醇浓度检测试剂盒及其制备方法 |
CN102520150A (zh) * | 2011-12-08 | 2012-06-27 | 上海高丰医疗电器有限公司 | 一种伽马-羟基丁酸的测定诊断试纸及其制备方法 |
EP2636750A1 (en) * | 2012-03-06 | 2013-09-11 | Roche Diagniostics GmbH | Compatible solute ectoine as well as derivatives thereof for enzyme stabilization |
CN102636651A (zh) * | 2012-04-06 | 2012-08-15 | 上海领潮生物科技有限公司 | 多项心肌标志物并行检测方法及系统、芯片试纸 |
US8921061B2 (en) * | 2012-11-02 | 2014-12-30 | Roche Diagnostics Operations, Inc. | Reagent materials and associated test elements |
US8920628B2 (en) * | 2012-11-02 | 2014-12-30 | Roche Diagnostics Operations, Inc. | Systems and methods for multiple analyte analysis |
WO2014087255A2 (en) * | 2012-11-21 | 2014-06-12 | Oslo Universitetssykehus Hf | Systems and methods for monitoring biological fluids |
US9121050B2 (en) | 2013-03-15 | 2015-09-01 | American Sterilizer Company | Non-enzyme based detection method for electronic monitoring of biological indicator |
US8858884B2 (en) | 2013-03-15 | 2014-10-14 | American Sterilizer Company | Coupled enzyme-based method for electronic monitoring of biological indicator |
CN104730230B (zh) * | 2013-12-23 | 2016-08-17 | 上海复星医药(集团)股份有限公司 | D-3-羟丁酸酶法检测试剂盒及其制备方法 |
JP6998658B2 (ja) * | 2014-04-17 | 2022-01-18 | ユニバーシティ オブ メリーランド, カレッジ パーク | アミノ酸代謝異常の検出のためのデバイス、及びデバイスを使用する方法 |
EP3172570A4 (en) | 2014-07-25 | 2017-12-27 | Becton, Dickinson and Company | Analyte test strip assays, and test strips and kits for use in practicing the same |
JP6815335B2 (ja) | 2016-02-04 | 2021-01-20 | テルモ株式会社 | 血糖値測定試薬、血糖値測定チップ、及び血糖値測定装置セット |
DK3423591T3 (da) * | 2016-03-04 | 2024-01-29 | Abbott Diabetes Care Inc | NAD(P)-afhængige sensitive enzymer, elektroder og sensorer og fremgangsmåder til fremstilling og anvendelse deraf |
KR101974645B1 (ko) * | 2016-12-02 | 2019-05-02 | 에스디 바이오센서 주식회사 | 전체-헤모글로빈 및 포도당-6-인산 탈수소효소 동시 측정용 스트립, 그 제조방법 및 그 응용 |
CN108745429B (zh) * | 2018-06-12 | 2023-11-24 | 南京岚煜生物科技有限公司 | 一种多通道快速检测微流体检测芯片 |
KR102079783B1 (ko) * | 2019-07-03 | 2020-02-21 | 주식회사 원드롭 | 생체 물질을 측정하기 위한 스트립 |
US11808708B2 (en) | 2020-08-12 | 2023-11-07 | F.A.T. Stats LLC | Method for maintaining the health of a diabetic patient by preventing the occurrence of diabetic ketoacidosis |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4254222A (en) * | 1978-07-19 | 1981-03-03 | Owen Oliver E | Semi-quantitative assay of lactic acid and β-hydroxy butyrate |
JPS59162899A (ja) * | 1983-03-08 | 1984-09-13 | Kyoto Daiichi Kagaku:Kk | β‐ヒドロキシ酪酸の定量用組成物 |
JPS60251895A (ja) * | 1984-05-29 | 1985-12-12 | Ube Ind Ltd | ピロロキノリンキノンの製造方法 |
JPH064038B2 (ja) * | 1986-01-29 | 1994-01-19 | 宝酒造株式会社 | L−フコ−スの定量方法 |
US4937047A (en) * | 1986-04-01 | 1990-06-26 | Konishiroku Photo Industry Co., Ltd. | Analytical element |
US5036000A (en) * | 1986-12-16 | 1991-07-30 | Enzymatics, Inc. | Threshold color control system |
US4970171A (en) * | 1987-11-09 | 1990-11-13 | Miles Inc. | Denaturant reagents for convenient determination of hemoglobin derivatives in blood |
SE466158B (sv) * | 1989-04-25 | 1992-01-07 | Migrata Uk Ltd | Analysmetod foer glukosbestaemning i helblod |
US5236567A (en) * | 1989-05-31 | 1993-08-17 | Nakano Vinegar Co., Ltd. | Enzyme sensor |
US5126275A (en) * | 1990-09-19 | 1992-06-30 | Miles Inc. | Analytical method using tetrazolium salt indicators having a reflectance plateau |
US5510245A (en) * | 1992-09-08 | 1996-04-23 | Bayer Corporation | Composition and method of assaying for ketone bodies |
US5298144A (en) * | 1992-09-15 | 1994-03-29 | The Yellow Springs Instrument Company, Inc. | Chemically wired fructose dehydrogenase electrodes |
DE4311464A1 (de) * | 1993-04-08 | 1994-10-13 | Boehringer Mannheim Gmbh | Verfahren zur kolorimetrischen Bestimmung eines Analyten mit einer PQQ-abhängigen Dehydrogenase |
CA2127172C (en) * | 1993-08-05 | 1998-07-14 | Amy H. Chu | Analyte detection device and process |
US5360595A (en) * | 1993-08-19 | 1994-11-01 | Miles Inc. | Preparation of diagnostic test strips containing tetrazolium salt indicators |
US5902731A (en) * | 1998-09-28 | 1999-05-11 | Lifescan, Inc. | Diagnostics based on tetrazolium compounds |
-
1998
- 1998-09-28 US US09/161,876 patent/US5902731A/en not_active Expired - Lifetime
-
1999
- 1999-03-08 EP EP99301727A patent/EP0990705B1/en not_active Expired - Lifetime
- 1999-03-08 DE DE69902612T patent/DE69902612T2/de not_active Expired - Lifetime
- 1999-03-08 AT AT99301727T patent/ATE222961T1/de active
- 1999-03-08 DK DK99301727T patent/DK0990705T3/da active
- 1999-03-08 PT PT99301727T patent/PT990705E/pt unknown
- 1999-03-08 ES ES99301727T patent/ES2183481T3/es not_active Expired - Lifetime
- 1999-03-09 IL IL12890599A patent/IL128905A/en not_active IP Right Cessation
- 1999-03-09 MY MYPI99000847A patent/MY117657A/en unknown
- 1999-03-09 ZA ZA9901906A patent/ZA991906B/xx unknown
- 1999-03-10 CA CA002265201A patent/CA2265201A1/en not_active Abandoned
- 1999-03-10 RU RU99104918/15A patent/RU2225004C2/ru active
- 1999-03-11 NO NO991185A patent/NO991185L/no unknown
- 1999-03-11 AU AU20373/99A patent/AU754321B2/en not_active Expired
- 1999-03-12 AR ARP990101091A patent/AR014718A1/es active IP Right Grant
- 1999-03-13 CN CNB991057406A patent/CN1155829C/zh not_active Expired - Lifetime
- 1999-03-15 JP JP11068545A patent/JP2000093198A/ja active Pending
- 1999-03-19 KR KR1019990009381A patent/KR100570909B1/ko not_active IP Right Cessation
- 1999-03-22 BR BR9901186-7A patent/BR9901186A/pt not_active IP Right Cessation
- 1999-03-30 US US09/282,083 patent/US6200773B1/en not_active Expired - Lifetime
- 1999-04-23 TW TW088106574A patent/TW558638B/zh active
- 1999-04-26 ZA ZA9902952A patent/ZA992952B/xx unknown
- 1999-04-26 IL IL12959499A patent/IL129594A/en not_active IP Right Cessation
- 1999-04-27 RU RU99109103/15A patent/RU2225005C2/ru active
- 1999-04-27 DE DE69902730T patent/DE69902730T2/de not_active Expired - Lifetime
- 1999-04-27 AT AT99303260T patent/ATE223497T1/de active
- 1999-04-27 ES ES99303260T patent/ES2183482T3/es not_active Expired - Lifetime
- 1999-04-27 PT PT99303260T patent/PT990706E/pt unknown
- 1999-04-27 EP EP99303260A patent/EP0990706B1/en not_active Expired - Lifetime
- 1999-04-27 DK DK99303260T patent/DK0990706T3/da active
- 1999-04-28 NO NO992026A patent/NO992026L/no not_active Application Discontinuation
- 1999-04-28 CA CA002270334A patent/CA2270334A1/en not_active Abandoned
- 1999-04-29 AR ARP990101993A patent/AR016241A1/es unknown
- 1999-05-03 SG SG9902107A patent/SG82610A1/en unknown
- 1999-05-06 MY MYPI99001790A patent/MY116028A/en unknown
- 1999-05-08 CN CNB991080904A patent/CN1142998C/zh not_active Expired - Lifetime
- 1999-05-10 JP JP11128731A patent/JP2000093199A/ja not_active Withdrawn
- 1999-05-11 KR KR1019990016786A patent/KR20000022630A/ko active IP Right Grant
- 1999-05-11 BR BR9901431-9A patent/BR9901431A/pt not_active Application Discontinuation
- 1999-06-16 TW TW088110056A patent/TW594009B/zh not_active IP Right Cessation
- 1999-08-26 AU AU44743/99A patent/AU763065B2/en not_active Expired
-
2000
- 2000-08-14 HK HK00105056A patent/HK1026001A1/xx not_active IP Right Cessation
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
TW558638B (en) | Diagnostic based on tetrazolium compounds | |
ES2262761T3 (es) | Composiciones de reactivo de tetrazolio estabilizado y procedimientos para usar las mismas. | |
AU628751B2 (en) | Composition and method of assaying for ketone bodies | |
CA2412198A1 (en) | Stabilized tetrazolium-phenazine reagent compositions and methods for using the same | |
CA2475098A1 (en) | Method and test strips for the measurement of fat loss during weight loss programs | |
RU2266543C2 (ru) | Реагентные системы для детектирования восстановленного кофактора | |
MXPA99004096A (en) | Diagnostic agent based on tetrazo compounds |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
GD4A | Issue of patent certificate for granted invention patent |