TW201507733A - Moisturizing agent - Google Patents

Abstract

The present invention provides an excellent moisturizing agent, which includes the following components (A) and (B): (A) a sugar fermentation product generated by using a yeast belonging to Kluyveromyces; and (B) a polyhydric alcohol having a specific gravity of above 1.02 at 20 DEG C, which can be selected from at least one of the glycerol, propanediol, propylene glycol, and dipropylene glycol. In the moisturizing agent of this invention, the dry solid content of component (A) can be set to be above 0.0001 mass% and below 0.09 mass%. Furthermore, the ratio of the dry solid content of component (A) to the content of component (B) can be set to be 0.0000025-0.9:1.

Description

moisturizer

The present invention relates to a humectant.

The moisturizing component is intended to impart moisture to the skin and hair, and to impart a moisturizing feeling to the skin, hair, and the like, and to blend a skin external preparation, a cosmetic, or a medical external product.

If the moisture in the stratum corneum of the outermost layer of the skin epidermis is reduced and the moisturizing function is lowered, the skin barrier function is also easily lowered. If the skin barrier function is lowered, skin problems such as dry skin, wrinkles, and rough skin are likely to occur, and the improvement of skin inflammation becomes sluggish.

Therefore, since the moisture of the stratum corneum is increased and maintained, it is related to keeping the skin soft, maintaining elasticity, and protecting the dermis, and is expected to be used for various purposes. As such a moisturizing component, for example, glycerin having high hygroscopicity or the like has been used. However, these components are easily affected by the surrounding humidity, and not only do not exert sufficient effects in dry skin, but also have the problem of "taking water from the skin and promoting drying".

Therefore, components mainly derived from living organisms or similar components of living organisms are attracting attention today. Specifically, it is a sodium lactate, an amino acid (for example, glycine, β-alanine, etc.) of NMF (Natural MOISTURIZING FACTOR), a polymer compound such as hyaluronic acid or soluble collagen, or a neuropterin or the like. .

For example, in Patent Document 1, a humectant containing an amidino-β-alanine acid or a salt thereof is proposed.

However, research and development of more moisturizing ingredients are underway as users demand various moisturizing ingredients.

[Previous Technical Literature] [Patent Literature]

[Patent Document 1] International Publication No. 2008/126652

Accordingly, the present invention is intended to provide an excellent moisturizer in view of the foregoing.

As a result of intensive research on a substance exhibiting a moisturizing effect, the inventors of the present invention have learned that a specific sugar fermented product which has hitherto been considered to exhibit a moisturizing effect is not displayed at all or reduced in moisturizing effect as the case may be. However, by using a polyol having a specific specific gravity in combination, the moisturizing effect can be greatly improved irrespective of the blending amount of the aforementioned sugar fermented product, and the present invention has been completed.

That is, the present invention provides a humectant comprising the following components (A) and (B); (A) is a sugar ferment produced by a yeast of the genus Kluyveromyces, and (B) is at 20 ° C. A polyol having a specific gravity of 1.02 or more.

In the humectant according to the present invention, as the sugar fermented product, a sugar fermented product derived from sugar cane can be used.

In the moisturizing agent related to the present invention, the dry solid form of the aforementioned component (A) The content of the fraction can be set to 0.0001% by mass or more and 0.09% by mass or less.

Further, the content ratio of the dry solid content of the component (A) to the component (B) may be set to 0.0000025 to 0.9:1.

In the humectant according to the present invention, the polyol as the component (B) may be one or more selected from the group consisting of glycerin, propanediol, propylene glycol, and dipropylene glycol. .

According to the present invention, an excellent moisturizing agent can be provided.

Fig. 1 is a graph showing the results of a test for confirming the moisturizing effect of the brown sugar fermentation broth and the polyol (the rate of change when 1 was applied before coating) in Experimental Example 1.

Fig. 2 is a graph showing the results of the test for confirming the moisturizing effect (comparison with the aqueous polyol solution) of the brown sugar fermentation broth and the polyol in Experimental Example 1.

Fig. 3 is a schematic diagram showing the rate of change of the moisture content after 60 minutes when the amount of water before application was taken as 1 in Experimental Example 2.

Fig. 4 is a schematic diagram showing the rate of change of the moisture content after two weeks of continuous use when the amount of water before application was taken as 1 in Experimental Example 3.

[Formation of the Invention]

The humectant of the present invention contains the component (A) a sugar fermented product produced by a yeast of the genus Kluyveromyces, and a polyhydric alcohol having a specific gravity of 1.02 or more at 20 ° C as the active ingredient.

In the present invention, "the component (A) is a sugar fermented product produced by a yeast of Kluyveromyces" (hereinafter also referred to as "ingredient (A) sugar fermented product").

The yeast of the genus Kluyveromyces used in the present invention is not particularly limited, and one or two or more kinds of the yeast species exemplified below can be used.

The above-mentioned "Kluyveromyces yeast" may, for example, be Kluyveromyces thermotolerans, Kluyveromyces africanus, Kluyveromyces fragilis, Kluyveromyces lactis, Kluyveromyces phaffii, Kluyveromyces waltii, Kluyveromyces wickerhamii, Kluyveromyces cerevisiae Kluyveromyces yarrowii) and so on.

Among them, Kluyveromyces thermotolerans, Kluyveromyces lactis, and Kluyveromyces fragilis are preferred, and Kluyveromyces thermotolerans are more preferred.

Furthermore, these yeasts have been used for the production of fermented foods such as dairy products, wines, and pickles, such as cheese and yogurt, since ancient times. For example, Kluyveromyces cerevisiae is a lactic acid-producing yeast isolated from wine brewing or plum jam ferment, and its biochemical and physiological properties have been reported (Reference 1: The Yeasts. A Taxonomic Study, 4th ed. Elsevier Science BV, Amsterdam. 240-241 (1998)). Further, as Kluyveromyces thermotolerans, Kluyveromyces thermotolerans MA KT (NITE BP-1420) strain can be mentioned.

As described above, since the Kluyveromyces yeast used in the present invention is a microorganism which is extremely safe to the human body and the sugar of the fermentation raw material can be eaten, it is found that the component (A) of the present invention has high safety of the sugar fermented product.

In the present invention, the "sugar" used as the raw material of the component (A) sugar fermented product is not particularly limited, but it is preferred to contain sucrose. The "sugar" may be in any form such as a powder or a liquid.

In the present invention, as the component (A), sugar derived from sugar cane is preferred, and cane sugar obtained from juice extraction of sugar cane stem is more preferred. The sugar from the sugar cane may, for example, be an unrefined sugar such as a three-pot sugar, a molasses, a black honey, a honey-containing sugar, a crude sugar or a brown sugar; or a refined sugar such as a white sugar or a sugar, or the like. One or two or more. Among them, unrefined sugar which is not purified to the upper white sugar is preferred, and among the unrefined sugar, brown sugar is preferred. Black sugar is suitable for heating and extracting sugar cane stems, and it is suitable for separation and purification without sugar.

In the present invention, "sugar cane" means a plant of the genus Sugarcane (Saccharum officinarum), and the Japanese "salt" is a sugar cane.

In the present invention, the component (A) sugar fermented product is preferably a fermented product obtained by culturing Kluyveromyces yeast in a medium containing sugar.

In the present invention, the content of the sugar in the sugar-containing medium is not particularly limited, but is preferably from 3 to 15% by mass, more preferably from 4 to 8% by mass.

Further, in the present invention, the medium containing sugar is preferably a liquid. The liquid medium containing the sugar may, for example, be an aqueous sugar solution obtained by mixing the above-mentioned sugar with water. The water is not particularly limited, and examples thereof include pure water, tap water, well water, mineral water, mineral water, hot spring water, mountain spring water, fresh water, purified water, ion exchange water, physiological saline, and phosphate buffer. Phosphate buffered physiological saline and the like.

In addition, the sugar-containing medium is preferably one containing a medium component (for example, a carbon source, a nitrogen source, an inorganic salt, or the like) which is usually used in yeast from the viewpoint of improving the productivity of the fermented product. Further, the sugar-containing medium is preferably used in a sterilization treatment (for example, heating or film treatment) from the viewpoint of preventing the growth of the bacteria.

The culture method of the Kluyveromyces yeast is not particularly limited as long as it is usually used for yeast, and can be carried out according to a known method (for example, refer to Reference 1).

For example, shaking culture, aeration culture, static culture, stirring culture, and the like can be mentioned. Also, it may be either batch type or continuous type. These may be carried out singly or in combination of two or more kinds.

The culture conditions of the Kluyveromyces yeast (for example, aerobic property, anaerobic property, pH in a medium, dissolved oxygen, culture temperature, culture time, and the like) are not particularly limited and may be appropriately selected depending on the purpose.

In the present invention, the component (A) sugar fermented product is preferably a gas-containing culture medium inoculated with a pre-cultured Kluyveromyces yeast, and is preferably aerobically cultured and prepared. At this time, it is preferable to add the culture solution before adding 1% with respect to the medium containing the sugar. In addition, the pH of the medium is adjusted to 5 to 7, and at 15 to 35 ° C, the aerobic culture is carried out for 1 to 3 days. Suitable. For the appropriate culture, it is preferred to carry out shaking culture or agitation culture under aerobic conditions. Further, in the case of aerobic conditions, it is sufficient to use air (oxygen about 20% by volume) as a sterilization filter.

Further, in the present invention, the preparation of the sugar fermented product is not actively subjected to ethanol fermentation as in the case of alcohol production, but the ethanol concentration in the sugar fermented product after the preparation is finished is less than 1% by mass (preferably 0.1 mass). % or less) is more suitable from the viewpoint of moisturizing effect.

In the present invention, the component (A) sugar fermented product may be used in this state or in a dry state after the above preparation. Further, after the preparation of the sugar fermented product, the cells of the Kluyveromyces yeast can be removed by a usual method such as centrifugation or membrane treatment.

Further, in the present invention, the component (A) sugar fermented product may be prepared as a diluent, a concentrate, or a dried product depending on the purpose, and may be further prepared as a separated or purified product or a purified product. A physiological saline solution can be used for dilution, and when dried, a freeze-drying method or a spray drying method can be used. In the case of separation and purification, for the purpose of removing impurities, etc., a usual method such as a centrifugation method, a precipitation method, or a chromatography method may be used, and an object such as deodorization or decolorization may be used, and activated carbon, an adsorbent, an ion exchange resin, or the like may be used.

Further, two or more kinds of sugar fermented products obtained by different yeast species can be used in combination.

The polyol (the component (B) having a specific gravity (g/mL at 20 ° C) of 1.02 or more) (hereinafter also referred to as "component (B) polyol") used in the present invention is described. In the present invention, the component (B) can be prepared by selecting one or two or more kinds of the following components (B) of the polyol.

In the present invention, the component (B) "polyol having a specific gravity of 1.02 or more at 20 ° C" includes methylene glycol, ethylene glycol (ethylene glycol), propylene glycol, and propylene glycol (1,3-). Propylene glycol), diethylene glycol, methylene propylene glycol, 1,2-cyclopentanediol, 1,3-cyclopentanediol, neopentyl glycol, isoprene glycol, aminoethyl propylene glycol, 1, 2 - hexanediol, 1,6-hexanediol, triethylene glycol, dipropylene glycol, butoxydiethylene glycol, ethyl hexanediol, butyl ethyl propylene glycol, 1,10-decanediol , equol, condensed ethylene glycol, propylene glycol, glycerol, trimethoxyethane, trimethoxypropane, 1,2,6-hexanetriol, phytanetriol, pentaerythritol , diglycerin, polyglycerin, etc.

Further, in the present invention, the specific gravity (g/mL at 20 ° C) of the component (B) polyol may be 1.02 or more. However, by selecting a polyol of 1.03 or more, the sugar of the component (A) may be used. When the fermented product is used together, the moisturizing property is improved. Further, in the present invention, the upper limit of the specific gravity (g/mL, at 20 ° C) of the polyol is not particularly limited, but from the viewpoint of preventing excessive viscous of the humectant, it is preferably 2 or less.

The carbon number of the polyol of the component (B) is preferably 6 or less, and more preferably 2 to 4.

The refractive index of the polyol of the component (B) is preferably from 1.3 to 1.6, more preferably from 1.4 to 1.5.

Among the above-mentioned component (B) polyols, propylene glycol, propylene glycol, dipropylene glycol and glycerin are more preferable from the viewpoint of improving moisture retention.

Further, in the humectant according to the present invention, in addition to the polyol having a specific gravity of 1.02 or more at 20 ° C, the component (B) may be freely Other polyols commonly used in humectants are used. Examples of the polyol other than the polyol having a specific gravity of 1.02 or more at 20 ° C as the component (B) include 1,2-butanediol, 1,3-butanediol, and 1,4-butanediol. 2,3-butanediol, 1,2-pentanediol, 1,3-pentanediol, 1,4-pentanediol, 1,5-pentanediol, 2,4-pentanediol, and the like.

In addition, the "specific gravity at 20 °C" of the polyol is a value measured in accordance with the first method of the specific gravity measurement method described in the Ministry of Health, Labour and Welfare of the Ministry of Health, Ministry of Health, and the Ministry of Health, 2006.

In addition, the "refractive index at 20 ° C" of the polyol is a value measured in accordance with the refractive index measurement method described in the Ministry of Health, Labor and Welfare of the Ministry of Health and the Ministry of Health, 2006.

The preparation of the humectant of the present invention is carried out by combining the above-mentioned component (A) sugar fermented product with the above-mentioned component (B) polyol, and therefore, it is sufficient to mix these. When these are combined, the above-mentioned component (A) sugar fermented product is preferably a sugar fermentation liquid (preferably an aqueous solution).

The content of the component (A) sugar fermented product in the moisturizing agent according to the present invention is preferably 0.0001 to 0.09% by mass, more preferably 0.001, as a dry solid content from the viewpoint of stability of the preparation and moisture retention. ~0.09% by mass, and more preferably 0.005 to 0.09% by mass.

The content of the component (B) polyol in the humectant according to the present invention is preferably from 0.1 to 40% by mass, more preferably from 1 to 25% by mass, further preferably from the viewpoint of safety and moisture retention. 5~10% by mass.

In the humectant of the present invention, the content ratio of the component (A) sugar fermented product to the component (B) polyol (parts by mass of the dry solid content: parts by mass) is not particularly limited, but from the viewpoint of improving moisture retention , It is preferably 0.000025 to 0.9:1, more preferably 0.00004 to 0.09:1, still more preferably 0.0005 to 0.018:1.

Further, the humectant can be adjusted to 100% by mass using water.

Further, the form of the humectant of the present invention obtained is not particularly limited, but a liquid or semi-solid shape is preferred.

As shown in the examples to be described later, it was confirmed that the moisture retaining property was rapidly increased as compared with the case where the component (A) sugar fermented product and the component (B) polyol were contained, respectively. Therefore, the mixture of the above-mentioned component (A) and the above-mentioned component (B) can be used for moisturizing purposes because of its excellent moisturizing property, and can also be used for various preparations for the purpose of moisturizing use. It can be used for the manufacture of these various formulations.

Further, since the combination of the component (A) and the component (B) has excellent moisturizing properties, it can be used for preventing, improving, and/or treating various skin-related symptoms or states by moisturizing. Methods. Examples of various symptoms and conditions include rough skin, wrinkle formation, skin aging, dry skin disease, and scalp. Further, the combination of the component (A) and the component (B) imparts a moist feeling to an object (for example, skin, hair, food, etc.). For example, the combination of the component (A) and the component (B) can be used for imparting a moist feeling or a compliant feeling to the hair, whereby the hairiness of the hair can be suppressed.

Therefore, the component (A) sugar fermented product and the component (B) polyol may be contained in an external preparation for skin, a cosmetic, a pharmaceutical product, a pharmaceutical, a food, or a functional food in order to enhance or impart moisture retention properties ( Specific Health foods, etc. (hereinafter also referred to as "formulations"). The moisturizing agent of the present invention can be used as the above-mentioned skin external preparation, cosmetic, food, and the like. Further, the moisturizing agent containing the component (A) and the component (B) of the present invention may be contained in these preparations.

The humectant of the present invention is particularly suitable for use in a skin external preparation, a cosmetic, a medical product, and the like, and is suitable for a coating such as application to the skin and hair. Examples of the external preparation for skin and the cosmetic include a lotion, an emulsion (oil type in water), a cream (oil type in water), a cosmetic for face (face), a foundation, an ointment, a hair restorer, and hair. Protective materials, etc.

Further, in the moisturizing agent of the present invention and the preparation of the present invention, in addition to the above-mentioned component (A) sugar fermented product of the present invention and the above-mentioned component (B) polyol, it is possible to have a range which does not impair the effects of the present technology. Combine any ingredients to use. The other component may be a pharmaceutically acceptable component, and examples thereof include a preservative, a cell activator, an antioxidant, a moisturizer, an ultraviolet preventive agent, a solvent (water, an alcohol, etc.), an oil agent, and an interface. Active agent, tackifier, powder, chelating agent, pH adjuster, emulsifier, stabilizer, colorant, brightener, flavoring agent, odorant, excipient, binder, breaker, lubricant, The diluent, the osmotic pressure adjusting agent, the fragrance, and the like may be blended in accordance with the intended preparation.

Further, the form of the preparation is not particularly limited, and may be any form such as a liquid, a paste, a gel, a solid, or a powder.

In the preparation, the amount of the humectant of the present invention is preferably from 0.01 to 40% by mass, more preferably from 0.1 to 25% by mass, still more preferably from 1 to 10% by mass.

Furthermore, the present technology can also adopt the following constitution.

[1] A humectant comprising the following components (A) and (B); (A) is a sugar fermented product produced by a yeast of the genus Kluyveromyces, and (B) is a specific gravity at a temperature of 20 ° C of 1.02 or more. Polyol.

[2] The humectant according to the above [1], wherein the sugar fermented product is a fermented product derived from cane sugar.

[3] The humectant according to the above [1] or [2] wherein the sugar fermented product has an ethanol concentration of less than 1% by mass.

[4] The humectant according to any one of the above [1] to [3] wherein the content of the dry solid content of the component (A) is 0.0001% by mass or more and 0.09% by mass or less.

[5] The humectant according to any one of the above [1] to [4] wherein the content ratio of the dry solid content of the component (A) to the component (B) is 0.0000025 to 0.9:1.

[6] The humectant according to any one of the above [1], wherein the polyol of the component (B) is one or more selected from the group consisting of glycerin, propylene glycol, propylene glycol, and dipropylene glycol. .

[7] The humectant according to any one of the above [1] to [6] wherein the yeast of the genus Kluyveromyces is selected from the group consisting of Kluyveromyces thermotolerans and Kluyveromyces. One or more of lactis) and Kluyveromyces fragilis.

[8] The humectant according to any one of the above [1] to [7] wherein the component (A) sugar fermented product is a Kluyveromyces yeast in a medium containing a sugar, preferably an unrefined sugar. The fermented product obtained by the cultivation.

[Examples]

Hereinafter, the present invention will be specifically described by way of examples, reference examples, comparative examples, test examples, production examples, and the like, but the present invention is not limited by the examples and the like.

<Preparation Example 1: Preparation of brown sugar fermentation broth produced by Kluyveromyces thermotolerans>

[pre-cultivation]

K. thermotolerans MA KT (NITE BP-1420) was cultured in liquid using YPD medium (pH 6.0). The above liquid culture solution of the platinum ring was taken, and the cells were cultured in YM medium (solid medium), and cultured at 25 ° C for 2 days. Further, YPD (Yeast Extract-Peptone-Dextrose) medium and YM (Yeast Mold) medium were prepared in accordance with the contents described in DifcoTM & BBLTM Manual, 2nd Edition.

Kluyveromyces thermotolerans MA KT (NITE BP-1420) strain, obtained in the country of origin: Japan / Separation source: sap, classified as heat-resistant Kluyveromyces cerevisiae by morphological observation and bacteriological properties (Lachancea (Kluyveromyces) thermotolerans) and named as the MA KT strain. This strain was deposited by Maruzen Pharmaceutical Co., Ltd. on September 13, 2012 at the storage location: Japan Post District No. 292-0818, 2-5-8, Kazuyoshi, Kisarazu, Chiba Prefecture, and an independent administrative agency product evaluation technology base institution. Patent Microbiology Depository Center (NPMD).

[Modulation of aqueous solution of brown sugar]

500 parts by mass of pure water was added to 25 parts by mass of brown sugar to form a 5% by mass aqueous solution of brown sugar. Mixing the aforementioned 5 mass% aqueous solution of black sugar with aeration After the mixed fermentation tank is sterilized together, it is adjusted to an appropriate temperature.

In addition, in the case of brown sugar, a commercial product produced by heating and concentrating the twisted juice of sugar cane stems produced in Kagoshima Prefecture is used.

[fermentation step]

The colony obtained by the culture of the aforementioned Kluyveromyces thermotolerans was suspended in physiological saline to prepare a suspension of 1.0×109 CFU/mL to 1.0×10 10 CFU/mL, and the total amount of the above-mentioned 5 mass% brown sugar aqueous solution was prepared. 1% by mass was added to the above-mentioned 5% by mass aqueous solution of brown sugar, and vibrating culture was carried out at 30 ° C for 2 days (vibration condition: 100 rpm). The culture solution after the vibration culture was sterilized in an autoclave (condition: 121 ° C, 15 minutes), and impurities, residues, and the like were removed by filtration. Then, purified water and phenoxyethanol were added to prepare a brown sugar fermentation liquid of Kluyveromyces thermotolerans having a solid concentration of 0.1% by mass. The ethanol content was 0.01%.

[Experimental Example 1]

In Experimental Example 1, the moisturizing effect of the sugar fermented product and the moisturizing effect when combined with various polyols were reviewed.

<Manufacture of humectants of samples 1 to 19>

As the polyol, the polyol shown in the following Table 1 was used.

The specific gravity is measured in accordance with the first method of the specific gravity measurement method described in the Ministry of Health, Labor and Welfare of the Ministry of Health and Welfare of the Ministry of Health of the People's Republic of China.

90% by mass of the black sugar fermentation liquid prepared in Preparation Example 1 (dry solid content concentration: 0.09% by mass), purified water, and various polyols in the amounts shown in Fig. 1 were mixed to prepare a moisturizer of Samples 1 to 19. Further, the residual amount is prepared by using purified water.

<Measurement of moisturizing effect>

The moisturizing effect was measured in accordance with the method described below.

After the inner part of the front wrist was washed, the measurement site (2 cm × 2 cm) was marked. The temperature was adjusted to 20 minutes at a temperature of 20 ° C and a humidity of 50%, and the amount of water was measured by Skicon 200 (I.B.S. Co., Ltd.). This value is used as the value before coating. Then, 100 μL of the sample was immersed in a toilet paper of 2 cm × 2 cm, placed in a measurement site, and allowed to stand for 5 minutes. Peel off the toilet paper and apply the sample to the skin with your fingers. After 60 minutes from the end of the coating, the amount of water was measured using Skicon 200 (I.B.S. Co., Ltd.), and the value was taken as the value after 60 minutes.

<Result>

The results of the moisturizing test are shown in Fig. 1 and Fig. 2.

Fig. 1 is a graph substitution diagram showing the rate of change of the moisture content after 60 minutes when the amount of water before coating is taken as 1.

Fig. 2 is a graph showing the substitution rate of the rate of change of the brown sugar fermentation broth/the rate of change of the purified water when the various polyols shown in the figure are used in combination. When the rate of change of the same amount of the aqueous polyol solution is taken as 1, the rate of change of the case where the brown sugar fermentation liquid is used in combination is expressed in parallel.

As shown in Fig. 1, it was found that when all of the samples 1 to 19 were applied, the amount of water after 60 minutes of application was increased as compared with that before application. However, it is also known that even if only purified water is applied, the amount of water after 60 minutes of application is increased compared to before application (see control group). On the other hand, if the control group of the purified water and the sample 1 of the purified water + brown sugar fermentation liquid are compared, it can be understood that the sample 1 to which the brown sugar fermentation liquid is added has a poor moisturizing effect (see Fig. 2).

Similarly, as shown in Fig. 2, when comparing purified water + various polyols and brown sugar fermentation liquid + polyol, samples 12 to 19 using a polyol having a specific gravity of less than 1.02 at 20 ° C, with or without The black sugar fermentation broth did not show any significant difference in moisturizing effect. However, in the samples 2 to 11 which used a polyol having a specific gravity of 1.02 or more at 20 ° C, it was confirmed that the combination of the brown sugar fermentation liquid and the moisturizing effect were used. The system is significantly improved.

From the above results, it can be seen that the sugar fermented product may not show or reduce the moisturizing effect at all, but by using the polyol having a specific gravity of 1.02 or more at 20 ° C, not only the moisturizing effect of the sugar fermented product is recovered, but also The moisturizing effect of the preparation such as the blended cosmetic is remarkably improved. That is, it can be understood that by using the component (A) sugar fermentate and component (B) together Alcohol will exert the moisturizing effect of combining the individual moisturizing effects of the above.

[Experimental Example 2]

In Experimental Example 2, the effect of the difference in the blending amount of the sugar fermented product and the polyol having a specific gravity of 1.02 or more at 20 ° C on the moisturizing effect was examined. Further, in the present experimental example, glycerin was used as an example of a polyol having a specific gravity of 1.02 or more at 20 °C.

<Manufacture of humectant of sample 20~24>

The humectant of Samples 20 to 24 was produced in accordance with the following Table 2 using the brown sugar fermentation broth prepared in Preparation Example 1.

<Measurement of moisturizing effect>

The moisturizing effect of the sample 20 to 24 produced as described above was measured in the same manner as in Experimental Example 1.

<Result>

The results are shown in Tables 2 and 3 below.

As shown in Tables 2 and 3, the sample 20 which was not blended with glycerin showed almost no moisturizing effect.

On the other hand, in the sample 22 and the sample 23, even if the amount of the brown sugar fermentation liquid was smaller than that of the sample 20, it was confirmed that the sample 21 containing the unblended brown sugar fermentation liquid was mixed with the moisturizing effect of the sample 20 which was not blended with glycerin. Moisturizing effect. Further, with respect to the sample 24 in which the brown sugar fermentation liquid equivalent to the sample 20 was blended, it was confirmed that the moisturizing effect was remarkably increased.

From the above results, it is understood that the sugar fermented product in an amount which hardly exhibits a moisturizing effect alone can be obtained by using a polyol having a specific gravity of 1.02 or more at 20 ° C to obtain a significantly enhanced moisturizing effect. That is, it can be understood that the polyol having a specific gravity of 1.02 or more at 20 ° C can significantly improve the moisturizing effect regardless of the blending amount of the sugar ferment.

[Experimental Example 3]

In Experimental Example 3, the moisturizing effect in the case where the moisturizing agent according to the present invention was continuously used was examined. The humectant of the sample 24 manufactured in Experimental Example 2 was used as the humectant of the present invention, respectively, and compared with the sample 21 manufactured in Experimental Example 2.

<Measurement of moisturizing effect>

The moisturizing effect by continuous use for 2 weeks was measured according to the method described below.

After the inner part of the front wrist was washed, the measurement site (2 cm × 2 cm) was marked. The water content was measured at a temperature of 20 ° C, a humidity of 50% for 20 minutes, and with a Skicon 200 (I.B.S. Co., Ltd.). This value is taken as the value before coating. Then, the sample 24 or the sample 21 is applied to the measurement site twice a day and two times a day. After the start of continuous use for 2 weeks, the temperature was adjusted to 20 minutes at a temperature of 20 ° C and a humidity of 50%, and the value of the moisture content measured by Skicon 200 (I.B.S. Co., Ltd.) was taken as a value after 2 weeks.

<Result>

The results are shown in Fig. 4. The rate of change of the moisture content after 2 weeks of continuous use of the water content before coating as 1 is shown in Fig. 4. It was observed that the sample 24 in which the glycerin and brown sugar fermentation broth was combined had a higher moisture content than the sample 21 in which glycerin and purified water were combined. This shows that in addition to the moisturizing effect, the skin is improved by continuous use, the moisture content of the skin itself is increased, and the roughness of the skin is improved.

[Experimental Example 4]

In Experimental Example 4, a solubilizing lotion, an emulsified lotion, an emulsion, a hair restorer, a hair care, and an ointment were produced using the moisturizing agent of the present invention.

[Prescription Example 1: Soluble lotion]

(Production method)

A. Mix and dissolve ingredients 1 to 6.

B. Mix and dissolve ingredients 7-11.

C. Add A to B to obtain a lotion.

The lotion of the prescription example 1 is intended to impart moisture to the skin and prevent dryness of the skin for a long period of time, and is excellent in moisturizing effect.

[Prescription Example 2: Emulsifying lotion]

(Production method)

A. Heat the ingredients 1 to 5 to 75 ° C and mix them evenly.

B. The ingredients 6, 7 were heated to 75 ° C and uniformly mixed and dissolved.

C. Add B to A to carry out emulsification.

D. C is cooled, and ingredients 8 to 15 are added to obtain an emulsified lotion.

The lotion of the prescription 2 is a moisturizing effect, and is effective for preventing moisturization of the skin for a long period of time.

[Prescription Example 3: Emulsion]

(Production method)

A: The component 16 was swollen with the component 17 heated to 70 °C.

B: Components 1 to 5 were heated and mixed at 70 °C.

C: Components 6 to 13 were dissolved by heating at 70 ° C, and then added to B to carry out emulsification.

D: After cooling C to room temperature, components 14, 15, 18, 19 and A were added to obtain a cosmetic liquid.

The emulsion of the formulation example 3 is intended to impart moisture to the skin and prevent dryness of the skin for a long period of time, and is excellent in moisturizing effect.

[Prescription Example 4: Hair restorer]

(Production method)

A. Mix and dissolve ingredients 1 to 6.

B. Mix and dissolve ingredients 7~13.

C. Add B to A to obtain a hair restorer.

2. The hair growth agent of this prescription 4 is given to the scalp. It is moisturized and prevents dryness for a long time. It is excellent for moisturizing effect.

[Prescription Example 5: Hair Care Agent]

(Production method)

A. Mix and dissolve ingredients 1 to 5.

B. Mix and dissolve ingredients 6 to 13.

C. Add B to A and add 14 to 17 to obtain a hair care agent.

2. The hair care agent of the prescription 5 is given to the scalp. The hair is moisturized, prevents drying for a long time, moisturizes the hair, and gives a good effect on the smoothness of the clothes.

[Prescription Example 6: Ointment]

(Production method)

A. Mix 1 to 5 at 70 ° C evenly.

B. Warm 6~8 to 70 °C.

C. Add B to A to carry out emulsification.

D. Cool C and add 9 to obtain an ointment.

The ointment of the present invention example 6 is intended to impart moisture to the skin, prevent dryness of the skin for a long period of time, and has an excellent moisturizing effect.

[registration acceptance number]

MA KT (NITE BP-1420) strain/classification: Lacancea (Kluyveromyces thermotolerans) / storage location: Japan Post District No. 292-0818 Kiyoshi-jin, Chiba Prefecture, 2-5-8, Separate Administration Legal Person Products Evaluation Technology Base Organization Patent Microbiology Deposit Center (NPMD) / Deposit Acceptance Date: September 13, 2012 / Depositor: Maruzen Pharmaceutical Co., Ltd.

Furthermore, the aforementioned strains can be freely transferred through the approval of the depositor.

Claims (5)

A humectant comprising the following components (A) and (B); (A) a sugar fermented product produced by a yeast of the genus Kluyveromyces, and (B) a polyhydric alcohol having a specific gravity of 1.02 or more at 20 °C. The humectant of claim 1, wherein the sugar ferment is a sugar fermented product from sugar cane. The humectant according to claim 1 or 2, wherein the content of the dry solid content of the component (A) is 0.0001% by mass or more and 0.09% by mass or less. The humectant according to any one of claims 1 to 3, wherein the ratio of the dry solid content of the component (A) to the component (B) is 0.0000025 to 0.009:1. The humectant according to any one of claims 1 to 4, wherein the polyol of the component (B) is selected from the group consisting of glycerin, propanediol, propylene glycol, and dipropylene glycol. Or two or more types.