TWI636797B - Moisturizer - Google Patents

Abstract

Provides excellent humectants.

Provide a humectant, which contains the following components (A) and (B); (A) is a sugar fermented product produced by a yeast belonging to the genus Kluyveromyces, and (B) the specific gravity at 20 ° C is 1.02 or more Polyol.

In the humectant according to the present invention, the dry solid content of the component (A) may be set to 0.0001% by mass or more and 0.09% by mass or less.

The content ratio of the dry solid content of the component (A) to the component (B) can be set to 0.0000025 to 0.9: 1.

Description

moisturizer

The present invention relates to a humectant.

The moisturizing component is for the purpose of adjusting the moisture on the skin surface and imparting a moist feeling to the skin, hair, and the like, and is blended with a skin external preparation, a cosmetic, or a quasi-drug.

If the moisture in the stratum corneum, which is the outermost layer of the skin epidermis, decreases and the moisturizing function is reduced, the skin barrier function is also easily reduced. If the skin barrier function is reduced, skin problems such as dry skin, wrinkles, and rough skin are likely to occur, and the improvement of skin inflammation will be delayed.

Therefore, increasing and maintaining the moisture in the stratum corneum is related to keeping the skin soft, maintaining elasticity, and protecting the dermis, and is expected to be used for various purposes. As such a moisturizing component, for example, glycerin having high hygroscopicity has been used. However, these ingredients are easily affected by the surrounding humidity, and not only do not exert sufficient effects in dry skin, but also have the problem of "capturing skin moisture and promoting dryness".

Therefore, nowadays, components mainly derived from organisms or similar components of organisms are receiving attention. Specifically, it uses sodium lactate, natural amino acids (e.g. glycine, β-alanine, etc.) or hyaluronic acid, soluble collagen, etc. using NMF (natural moisturizing factor; NATURAL MOISTURIZING FACTOR). .

For example, Patent Document 1 proposes a humectant containing lysamino-β-alanine or a salt thereof.

However, as users demand various moisturizing ingredients, more research and development of moisturizing ingredients are in progress.

[Prior technical literature] [Patent Literature]

[Patent Document 1] International Publication No. 2008/126652

Therefore, the present invention is intended to provide an excellent moisturizer in view of the foregoing facts.

As a result of intensive research on substances exhibiting a moisturizing effect, the inventors of the present application have learned that a specific sugar fermented substance which has been considered to have a moisturizing effect so far may not show or reduce the moisturizing effect at all depending on the situation. However, by using a polyhydric alcohol having a specific specific gravity in combination, the moisturizing effect can be greatly improved regardless of the amount of the aforementioned sugar fermentation product, and the present invention has been completed.

That is, the present invention provides a humectant, which contains the following components (A) and (B); (A) is a sugar fermentation product produced by a Kluyveromyces yeast, and (B) is at 20 ° C. Polyol having a specific gravity of 1.02 or more.

In the humectant according to the present invention, as the sugar fermentation product, a sugar fermentation product derived from sugar cane can be used.

In the humectant related to the present invention, the dry solid form of the aforementioned component (A) The content of the component can be set to 0.0001 mass% or more and 0.09 mass% or less.

The content ratio between the dry solid content of the component (A) and the component (B) can be set to 0.0000025 to 0.9: 1.

In the humectant related to the present invention, the polyhydric alcohol as the component (B) may be selected from one or two or more kinds of glycerin, propanediol, propylene glycol, and dipropylene glycol. .

According to the present invention, an excellent humectant can be provided.

FIG. 1 is a graph showing the results of a confirmation test of the moisturizing effect of brown sugar fermentation broth and a polyhydric alcohol (the rate of change when 1 was applied before application) in Experimental Example 1. FIG.

FIG. 2 is a graph showing the results of a confirmation test of the moisturizing effect (compared with a polyhydric alcohol aqueous solution) of a brown sugar fermentation broth and a polyhydric alcohol in Experimental Example 1. FIG.

FIG. 3 is a graph substitute graph showing the moisture content before coating as the rate of change of the moisture content after 1 minute and 60 minutes in Experimental Example 2. FIG.

FIG. 4 is a graph substitute graph showing the change rate of the moisture content before the continuous application after 2 weeks of continuous use at a moisture content of 1 before coating in Experimental Example 3. FIG.

[Form of Implementing Invention]

The humectant of the present invention is a sugar-fermented product produced by a yeast of the Kluyveromyces genus, and a polyhydric alcohol having a specific gravity of 1.02 or more at 20 ° C with the component (B) as an active ingredient.

The "component (A) sugar fermented product produced by a yeast of Kluyveromyces" used in the present invention (hereinafter also referred to as "component (A) sugar fermented product") will be described.

The "Kluyveromyces yeast" used in the present invention is not particularly limited, and one or more of the yeast species exemplified below may be used.

Examples of the “Kluyveromyces yeast” include, for example, Kluyveromyces thermotolerans, Kluyveromyces africanus, Kluyveromyces fragilis, Kluyveromyces lactis, Kluyveromyces phaffii, Kluyveromyces waltii, Kluyveromyces wickerhamii, Yarrow Kluyveromyces Kluyveromyces yarrowii), etc.

Among them, Kluyveromyces thermotolerans, Kluyveromyces lactis, and Kluyveromyces fragilis are preferred, and Kluyveromyces thermotolerans are more preferred.

In addition, these yeasts have been used in the manufacture of fermented foods such as dairy products such as cheese and yogurt, wine, and pickles since ancient times. For example, Kluyveromyces thermotolerance is a yeast produced from lactic acid produced by wine or plum jam fermentation, and its biochemical and physiological properties have been reported (Reference 1: The Yeasts. A Taxonomic Study, 4th ed. Elsevier Science BV, Amsterdam. 240-241 (1998)). Examples of the Kluyveromyces thermotolerans include Kluyveromyces thermotolerans MA KT (NITE BP-1420) strain.

In this way, the Kluyveromyces yeast used in the present invention is a microorganism which is extremely safe to human body and the sugar of the fermentation raw material is also edible. Therefore, the component (A) sugar fermented product of the present invention has high safety.

In the present invention, the "sugar" used as the raw material of the sugar fermented ingredient (A) is not particularly limited, but it is preferably one containing sucrose. "Sugar" may be in any form such as powder or liquid.

In the present invention, the component (A) is preferably sugarcane-derived sugar, and more preferably sugarcane sugar obtained from the juice of the sugarcane stem. As for the sugar derived from sugar cane, for example, unrefined sugar such as three bowls of sugar, molasses, black honey, honey, crude sugar, brown sugar, and the like; refined sugar such as white sugar, granulated sugar, and the like may be selected. One or more of them. Among them, unrefined sugar that has not been refined to white sugar is preferred, and brown sugar is more preferred among the unrefined sugars. Brown sugar is produced by heating the juice of the sugarcane stem and concentrating it. It is more suitable for those who have not separated and refined the sugar.

In the present invention, "sugar cane" means a plant belonging to the genus Sugarcane of the family Gramineae (Saccharum officinarum, scientific name: Saccharum officinarum). Japanese "glucan" is sugar cane.

In the present invention, the component (A) sugar fermentation product is preferably a fermentation product obtained by culturing a Kluyveromyces yeast in a sugar-containing medium.

In the present invention, the sugar content in the sugar-containing medium is not particularly limited, but it is preferably 3 to 15% by mass, and more preferably 4 to 8% by mass.

In the present invention, a sugar-containing medium is preferably a liquid. Examples of the sugar-containing liquid medium include, for example, an aqueous sugar solution in which the aforementioned sugar is mixed with water. The water is not particularly limited, and examples thereof include pure water, tap water, well water, mineral water, mineral water, hot spring water, mountain spring water, fresh water, purified water, ion-exchanged water, physiological saline, and phosphate buffer solution. , Phosphate buffered saline and so on.

In addition, the aforementioned sugar-containing medium is preferably one containing a medium component (for example, a carbon source, a nitrogen source, an inorganic salt, and the like) that is generally used in yeast from the viewpoint of improving the productivity of the fermented product. The sugar-containing medium is preferably a sterilizing treatment (for example, heating or membrane treatment) from the viewpoint of preventing the propagation of germs.

The above-mentioned Kluyveromyces yeast culture method is not particularly limited as long as it is a culture method generally used for yeast, and a known method can be used (for example, refer to Reference 1).

Examples thereof include shaking culture, aeration culture, standing culture, and stirring culture. Moreover, it may be either a batch type or a continuous type. These can be performed individually or in combination of 2 or more types.

The culture conditions of the aforementioned Kluyveromyces yeasts (for example, aerobic properties, aerobic properties, pH in the culture medium, dissolved oxygen, culture temperature, and culture time, etc.) are not particularly limited, and can be appropriately selected according to the purpose.

In the present invention, the component (A) sugar fermented product is a sugar-containing medium inoculated with a Kluyveromyces yeast cultured before the inoculation, and it is suitable to perform aerobic culture and preparation. At this time, it is more suitable to add 1% of the culture medium to the medium containing sugar. In addition, the pH of the medium was adjusted to 5 to 7, and the aerobic culture was performed at 15 to 35 ° C for 1 to 3 days. Suitable. For this suitable culture, it is preferable to perform shaking culture or agitation culture under aerobic conditions. In addition, in the case of aerobic conditions, what is necessary is just to use a person who treats air (oxygen about 20% by volume) with a sterilizing filter.

In addition, in the present invention, the preparation of the sugar fermented product is not to be actively fermented with ethanol as in the production of alcoholic beverages, but the ethanol concentration of the sugar fermented product after the preparation is finished to be less than 1 mass% (preferably 0.1 mass) % Or less) is more suitable from the viewpoint of moisturizing effect.

In the present invention, after the component (A) sugar fermentation product is prepared as described above, it can be used as it is or in a dry state. In addition, after the sugar fermentation product is prepared, the Kluyveromyces yeasts can be removed by a conventional method such as centrifugation or membrane treatment.

In the present invention, the component (A) sugar fermented product may be prepared into a dilute solution, a concentrated solution, or a dried product according to the purpose, or may be further prepared into an isolated product or a purified product. A physiological salt solution can be used for dilution, and a freeze-drying method or a spray-drying method can be used for drying. In the separation and purification, ordinary methods such as centrifugation, precipitation, and chromatography can be used for the purpose of removing impurities and the like, and deodorization and decoloration can be used for the purpose, and activated carbon, adsorbent, ion exchange resin, and the like can be used.

Further, two or more kinds of sugar fermented products obtained from different yeast strains can be used in combination.

The "polyol whose specific gravity (g / mL, at 20 ° C) at 20 ° C is 1.02 or more" used in the present invention (hereinafter also referred to as "component (B) polyol") will be described. In the present invention, the component (B) can be prepared by selecting one or two or more types from the following examples of the component (B) polyol.

In the present invention, the component (B) "polyol having a specific gravity at 20 ° C of 1.02 or more" includes methylene glycol, ethylene glycol (ethylene glycol), propylene glycol, and propylene glycol (1,3- Propylene glycol), diethylene glycol, methylene propylene glycol, 1,2-cyclopentanediol, 1,3-cyclopentanediol, neopentyl glycol, isoamyl glycol, aminoethyl propylene glycol, 1,2 -Hexanediol, 1,6-hexanediol, triethylene glycol, propylene glycol, butoxydiethylene glycol, ethylhexanediol, butylethylpropylene glycol, 1,10-decanediol , Equol, polyethylene glycol, polypropylene glycol, glycerol, trimethoxyethane, trimethoxypropane, 1,2,6-hexanetriol, phytanetriol, pentaerythritol , Diglycerin, polyglycerin, etc.

In the present invention, the specific gravity (g / mL, at 20 ° C.) of the polyol of the component (B) may be 1.02 or more. However, by selecting a polyol of 1.03 or more, it is possible to obtain a sugar with the component (A). When fermented material is used in combination, the moisturizing property is improved. In the present invention, the upper limit of the specific gravity (g / mL, at 20 ° C.) of the polyhydric alcohol is not particularly limited, but from the viewpoint of preventing excessive stickiness of the humectant, it is preferably 2 or less.

The carbon number of the polyol of the component (B) is preferably 6 or less, and more preferably 2 to 4.

The refractive index of the aforementioned component (B) polyol is preferably 1.3 to 1.6, and more preferably 1.4 to 1.5.

Among the above-mentioned component (B) polyols, propylene glycol, propylene glycol, propylene glycol, and glycerol are more preferable from the viewpoint of improving the moisturizing property.

In addition, in the humectant related to the present invention, in addition to a polyol having a specific gravity of 20% or higher at 20 ° C, the humectant can be freely combined with Use other polyols commonly used in humectants. Examples of the polyol other than the polyol having a specific gravity of 1.02 or more at 20 ° C include 1,2-butanediol, 1,3-butanediol, 1,4-butanediol, 2,3-butanediol, 1,2-pentanediol, 1,3-pentanediol, 1,4-pentanediol, 1,5-pentanediol, 2,4-pentanediol, and the like.

The "specific gravity at 20 ° C" of the polyol is a value measured in accordance with the first method of the specific gravity measurement method described in the Ministry of Health, Labour and Welfare: Raw Material Specifications 2006 (published by Pharmaceutical Affairs Daily).

The "refractive index at 20 ° C" of the polyol is a value measured in accordance with the refractive index measurement method described in the Ministry of Health, Labour and Welfare: Raw Material Standard 2006 (published by Pharmaceutical Affairs Daily).

The preparation of the moisturizing agent of the present invention is a combination of the aforementioned component (A) sugar fermented product and the aforementioned component (B) polyol, so it is only necessary to mix these. When combining these, it is preferable to use the sugar fermentation liquid (preferably an aqueous solution) as the sugar fermentation product of the said component (A).

The content of the aforementioned sugar fermentation product (A) in the humectant related to the present invention is preferably 0.0001 to 0.09 mass%, more preferably 0.001, as a dry solid content from the viewpoint of stability and moisture retention of the preparation. ~ 0.09 mass%, more preferably 0.005 to 0.09 mass%.

The content of the aforementioned component (B) polyol in the humectant of the present invention is preferably from 0.1 to 40% by mass, more preferably from 1 to 25% by mass, and even more preferably from the viewpoint of safety and moisturizing properties. 5 to 10% by mass.

In the moisturizing agent of the present invention, the content ratio of the aforementioned component (A) sugar fermented product to the aforementioned component (B) polyol (parts by mass of dry solid content: parts by mass) is not particularly limited, but from the viewpoint of improving moisturizing properties , It is preferably 0.0000025 to 0.9: 1, more preferably 0.00004 to 0.09: 1, and still more preferably 0.0005 to 0.018: 1.

The humectant can be adjusted to 100% by mass using water.

The form of the obtained moisturizing agent of the present invention is not particularly limited, but a liquid or semi-solid shape is preferred.

As shown in the examples described later, it is considered that the moisturizing property is rapidly improved by containing the above-mentioned component (A) sugar fermented product and the above-mentioned component (B) polyhydric alcohol, compared to the case where each of them is alone. Therefore, the mixture of the aforementioned component (A) and the aforementioned component (B) are various preparations which can be used for moisturizing purposes as well as being used for moisturizing purposes due to their very good moisturizing properties. It can be used for the manufacture of these various preparations.

The combination of the aforementioned component (A) and the aforementioned component (B) is excellent in moisturizing property, and thus can be used for moisturizing and intended to prevent, improve, and / or treat various symptoms or conditions related to the skin. Methods. Examples of the various symptoms and conditions include rough skin, wrinkle formation, skin aging, xeroderma, scalp and the like. Moreover, the combination of the said component (A) and the said component (B) can provide a moist feeling to an object (for example, skin, hair, food, etc.). For example, a combination of the aforementioned component (A) and the aforementioned component (B) can be used for the purpose of imparting a moisturizing feeling or a comfortable feeling to the hair, thereby suppressing the dryness of the hair.

Therefore, the component (A) sugar fermentation product and the component (B) polyol may be included in skin external preparations, cosmetics, external medicines, pharmaceuticals, foods, or functional foods in order to improve or impart moisturizing properties ( E.g. specific Health foods), etc. (hereinafter also referred to as "formulations"). The moisturizer of the present invention can be made into the skin external preparations, cosmetics, foods, and the like described above. Moreover, the moisturizer containing the said component (A) and the said component (B) of this invention can also be contained in these preparations.

The moisturizing agent of the present invention is particularly suitable for preparations for external use on skin, cosmetics, external products of the medical department, etc., and is suitable for contacting skin and hair. Examples of the external preparations for skin and cosmetics include lotions, lotions (oil-in-water type), creams (oil-in-water type), dressings (faces), foundations, ointments, hair growth agents, and hair Protective materials, etc.

In addition, in the humectant and the preparation of the present invention, in addition to the aforementioned component (A) sugar fermentation product and the aforementioned component (B) polyol of the present invention, as long as the effect of the present technology is not impaired, it may be required. Instead, use any combination of ingredients. The other components may be pharmaceutically acceptable components, and examples thereof include preservatives, cell activating agents, antioxidants, humectants, ultraviolet inhibitors, solvents (water, alcohols, etc.), oils, and interfaces. Active agents, thickeners, powders, chelating agents, pH adjusters, emulsifiers, stabilizers, colorants, gloss agents, flavoring agents, flavoring agents, excipients, binding agents, disintegrating agents, lubricants, The diluent, osmotic pressure regulator, perfume, and the like may be blended in accordance with the intended preparation.

Moreover, the form of the said preparation is not specifically limited, It can be any form, such as a liquid form, a paste form, a gel form, a solid form, and a powder form.

In the formulation, the amount of the humectant of the present invention is preferably 0.01 to 40% by mass, more preferably 0.1 to 25% by mass, and even more preferably 1 to 10% by mass.

In addition, this technology can also adopt the following configurations.

[1] A humectant, which contains the following components (A) and (B); (A) is a sugar fermented product produced by a yeast of the genus Kluyveromyces, and (B) has a specific gravity at 20 ° C of 1.02 or more Polyol.

[2] The humectant according to the above [1], wherein the sugar fermentation product is a fermentation product derived from sugar cane sugar.

[3] The humectant according to the above [1] or [2], wherein the sugar fermentation product is one having an ethanol concentration of less than 1% by mass.

[4] The humectant according to any one of the above [1] to [3], wherein the dry solid content of the component (A) is 0.0001% by mass or more and 0.09% by mass or less.

[5] The humectant according to any one of the above [1] to [4], wherein the content ratio of the dry solid content of the component (A) to the component (B) is 0.0000025 to 0.9: 1.

[6] The humectant according to any one of the above [1] to [4], wherein the polyol of the component (B) is one or more selected from the group consisting of glycerin, propylene glycol, propylene glycol, and propylene glycol .

[7] The humectant according to any one of [1] to [6], wherein the Kluyveromyces yeast is selected from the group consisting of Kluyveromyces thermotolerans and Kluyveromyces lactis) and one or more of Kluyveromyces fragilis.

[8] The humectant according to any one of the above [1] to [7], wherein the component (A) is a sugar fermentation product comprising a Kluyveromyces yeast in a medium containing sugar (preferably unrefined sugar) Fermented in medium culture.

[Example]

Hereinafter, examples, reference examples, comparative examples, test examples, manufacturing examples, and the like are listed to more specifically describe the present invention. However, the present invention is not limited by these examples and the like.

<Preparation Example 1: Preparation of brown sugar fermentation broth produced by Kluyveromyces thermotolerans>

[Pre-culture]

Kluyveromyces thermophila MA KT (NITE BP-1420) was subjected to liquid culture using YPD medium (pH 6.0). Take the above-mentioned liquid culture solution of 1 platinum-plating ring, plant the bacteria in YM medium (solid medium), and culture at 25 ° C for 2 days. In addition, YPD (Yeast Extract-Peptone-Dextrose) medium and YM (Yeast Mold) medium were prepared according to the contents described in the DifcoTM & BBLTM Manual, 2nd Edition.

Kluyveromyces thermotolerans MA KT (NITE BP-1420) strain, originating in country of origin: Japan / isolated source: sap, obtained by morphological observation and bacteriological properties and classified as Kluyveromyces thermotolerant (Lachancea (Kluyveromyces) thermomotolerans) and named the MA KT strain. This strain was deposited by Maruzen Pharmaceutical Co., Ltd. on September 13, 2012 at the storage location: Japan Post Code 292-0818, 2-5-8 Kamakami, Kisarazu, Chiba Prefecture, independent administrative corporation product evaluation technology base organization Patented Microbial Depository Center (NPMD).

[Preparation of brown sugar aqueous solution]

500 parts by mass of pure water was added to 25 parts by mass of brown sugar to form a 5% by mass brown sugar aqueous solution. Stir the aforementioned 5 mass% brown sugar aqueous solution with aeration After sterilizing the mixing-type fermentation tank together, adjust to an appropriate temperature.

In addition, as for brown sugar, a commercially available product manufactured by heating and concentrating the stalk of sugarcane stems from Kagoshima Prefecture is used.

[Fermentation step]

The colonies obtained by the previous culture of Kluyveromyces thermotolerans were suspended in physiological saline to prepare a suspension of 1.0 × 109 CFU / mL to 1.0 × 1010 CFU / mL, and the entire amount of the aforementioned 5 mass% brown sugar aqueous solution was prepared. 1% by mass was added to the aforementioned 5% by mass brown sugar aqueous solution, and vibration culture was performed at 30 ° C for 2 days (vibration conditions: 100 rpm). The culture liquid after shaking culture was sterilized in an autoclave (condition: 121 ° C, 15 minutes), and then impurities and residues were removed by filtration. Then, purified water and phenoxyethanol were added to prepare a brown sugar fermentation broth of Kluyveromyces thermotolerans having a solid content concentration of 0.1% by mass. The ethanol content was 0.01%.

[Experimental Example 1]

In Experimental Example 1, the moisturizing effect of the sugar fermentation product and the moisturizing effect when combined with various polyols were reviewed.

<Manufacture of humectants for samples 1 to 19>

As the polyol, a polyol shown in Table 1 below was used.

The specific gravity is measured in accordance with the first method of the specific gravity measurement method described in the Ministry of Health, Labour and Welfare: Raw Material Specifications 2006 (published by Pharmaceutical Daily).

90% by mass of the brown sugar fermentation broth (dry solids concentration 0.09% by mass) prepared in Preparation Example 1 or purified water and various polyols in the amounts shown in Fig. 1 were mixed to produce samples 1 to 19 as humectants. In addition, the residual amount is prepared with purified water.

<Measurement of Moisturizing Effect>

The moisturizing effect was measured according to the following method.

After the medial part of the forearm was washed, the measurement site (2 cm x 2 cm) was marked. Adapted at 20 ° C and 50% humidity for 20 minutes, and measured the water content using Skicon 200 (I.B.S. Co., Ltd.). This value was used as the value before coating. Then, 100 μL of the sample was impregnated with 2 cm × 2 cm of toilet paper, and then placed on the measurement site and left for 5 minutes. Peel off the toilet paper and apply the sample to the skin with your fingers. After 60 minutes from the end of coating, the moisture content was measured using Skicon 200 (I.B.S. Co., Ltd.), and the value was taken as the value after 60 minutes.

<Result>

The results of the moisturizing test are shown in FIG. 1 and FIG. 2.

Fig. 1 is a graph substitute graph showing the moisture content change rate after 60 minutes when the moisture content before coating is regarded as 1.

Fig. 2 is a graph substitute drawing showing a change rate of a brown sugar fermentation broth / a change rate of purified water when various polyols shown in the figure are used in combination. When the change rate of the same amount of the polyhydric alcohol aqueous solution is taken as 1, the change rates of the case where the brown sugar fermentation broth is used in parallel are shown together.

As shown in FIG. 1, it can be seen that when all the samples 1 to 19 are applied, the water content after 60 minutes of application is increased compared to that before application. However, it is also known that even if only purified water is applied, the water content is increased after 60 minutes of application compared to before application (refer to the control group). If you compare the control group with purified water only with sample 1 of purified water + brown sugar fermentation broth, it can be seen that sample 1 with brown sugar fermentation broth added has poor moisturizing effect (see Figure 2).

Similarly, as shown in FIG. 2, if purified water + various polyols are compared with brown sugar fermentation broth + polyol, samples 12 to 19 using polyols having a specific gravity of less than 1.02 at 20 ° C are used, with or without No significant difference in moisturizing effect was seen with the use of brown sugar fermentation broth. However, in samples 2 to 11 using a polyhydric alcohol having a specific gravity of 1.02 or higher at 20 ° C, the moisturizing effect was confirmed by the combination of brown sugar fermentation broth. Department has improved significantly.

From the above results, it can be seen that the sugar fermented product may not show or reduce the moisturizing effect at all. However, by using it with a polyhydric alcohol having a specific gravity of 1.02 or higher at 20 ° C, the moisturizing effect of the sugar fermented product is restored and The moisturizing effect of the blended preparations is significantly improved. That is, it can be understood that by using the component (A) sugar fermentation product and the component (B) in combination Alcohol has a moisturizing effect in which the individual moisturizing effects are combined and multiplied.

[Experimental Example 2]

In Experimental Example 2, the influence of the difference in the blending amount of the sugar fermented product and the polyhydric alcohol having a specific gravity of 1.02 or higher at 20 ° C on the moisturizing effect was examined. In this experimental example, glycerin is used as an example of a polyhydric alcohol having a specific gravity of 1.02 or higher at 20 ° C.

<Manufacture of humectants for samples 20 to 24>

Using the brown sugar fermentation broth prepared in Preparation Example 1, the humectants of samples 20 to 24 were prepared according to the following Table 2 below.

<Measurement of Moisturizing Effect>

Regarding the humectants of the samples 20 to 24 manufactured as described above, the moisturizing effect was measured in the same manner as in Experimental Example 1.

<Result>

The results are shown in Tables 2 and 3 below.

As shown in Tables 2 and 3, the sample 20 without glycerol showed almost no moisturizing effect.

On the other hand, even in samples 22 and 23, even if the amount of brown sugar fermentation broth was smaller than that of sample 20, it was confirmed that the moisturizing effect of sample 21 in which brown sugar fermentation broth was not blended and sample 20 without glycerol blend was more Moisturizing effect. In addition, it was confirmed that the moisturizing effect was remarkably increased in the sample 24 in which the brown sugar fermentation liquid equivalent to the sample 20 was blended.

From the above results, it can be seen that the sugar fermented product in an amount that hardly exhibits a moisturizing effect even when used alone can obtain a significantly increased moisturizing effect by being used in combination with a polyhydric alcohol having a specific gravity of 20 ° C or higher of 1.02 or more. That is, it can be understood that the moisturizing effect can be significantly improved by using it in combination with a polyhydric alcohol having a specific gravity of 1.02 or higher at 20 ° C, regardless of the amount of the sugar fermentation product.

[Experimental Example 3]

In Experimental Example 3, the moisturizing effect in the case where the moisturizer related to the present invention was continuously used was reviewed. The humectant of the sample 24 produced in Experimental Example 2 was used as a humectant related to the present invention, and was compared with the sample 21 produced in Experimental Example 2.

<Measurement of Moisturizing Effect>

The moisturizing effect by continuous use for 2 weeks was measured according to the following method.

After the medial part of the forearm was washed, the measurement site (2 cm x 2 cm) was marked. The temperature was adjusted at 20 ° C and humidity 50% for 20 minutes, and the moisture content was measured using Skicon 200 (I.B.S. Co., Ltd.). This value is taken as the value before coating. Then, sample 24 or sample 21 was applied to the measurement site twice a day, morning and evening. After 2 weeks of continuous use, it was conditioned for 20 minutes at an air temperature of 20 ° C and a humidity of 50%, and the value of the moisture content measured with Skicon 200 (I.B.S. Co., Ltd.) was taken as the value after 2 weeks.

<Result>

The results are shown in FIG. 4. The rate of change of the moisture content before coating is regarded as 1 at the time of continuous use for 2 weeks, and the change rate of the moisture content is shown in FIG. 4. It was observed that the water content of the sample 24 combining glycerol and brown sugar fermentation broth was higher than that of the sample 21 combining glycerol and purified water. This shows that in addition to the moisturizing effect, the skin is improved by continuous use, the moisture content of the skin is increased, and the rough skin is improved.

[Experimental Example 4]

In Experimental Example 4, a solubilizing lotion, an emulsified lotion, an emulsion, a hair restorer, a hair care agent, and an ointment were manufactured using the humectant related to the present invention.

[Prescription Example 1: Soluble Toner]

(Production method)

A. Mix and dissolve ingredients 1 ~ 6.

B. Mix and dissolve ingredients 7-11.

C. Add A to B to get a lotion.

The lotion of this prescription example 1 imparts moisturization to the skin, prevents dryness of the skin for a long time, and is excellent in moisturizing effect.

[Prescription Example 2: Emulsified toner]

(Production method)

A. Heat ingredients 1 to 5 to 75 ° C and mix and dissolve them uniformly.

B. Heat ingredients 6 and 7 to 75 ° C, and mix and dissolve them uniformly.

C. B is added to A and emulsified.

D. C is cooled, and ingredients 8 to 15 are added to obtain an emulsified lotion.

The lotion of this prescription example 2 imparts moisturization to the skin, prevents dryness of the skin for a long time, and is excellent in moisturizing effect.

[Prescription Example 3: Emulsion]

(Production method)

A: The component 16 is swollen with the component 17 heated to 70 ° C.

B: Ingredients 1 to 5 are heated and mixed at 70 ° C.

C: Components 6 to 13 are heated and dissolved at 70 ° C, and then added to B to emulsify.

D: After cooling C to room temperature, components 14, 15, 18, 19, and A were added to obtain a cosmetic lotion.

The lotion of this prescription example 3 gives skin moisturization, prevents skin dryness for a long time, and is excellent in moisturizing effect.

[Prescription example 4: Hair growth agent]

(Production method)

A. Mix and dissolve ingredients 1 ~ 6.

B. Mix and dissolve ingredients 7 ~ 13.

C. Add B to A to obtain a hair restorer.

The hair growth agent of Example 4 is given to the scalp. Hair is moisturized, prevents dryness for a long time, and is excellent in moisturizing effect.

[Prescription Example 5: Hair Care Agent]

(Production method)

A. Mix and dissolve ingredients 1 ~ 5.

B. Mix and dissolve ingredients 6-13.

C. Add B to A and add 14 to 17 to obtain a hair conditioner.

The hair care agent of Formula 5 is given to the scalp. Hair moisturizing, prevent dryness for a long time, moisturize hair, and give a good sense of comfort.

[Prescription example 6: ointment]

(Production method)

A. Mix 1 ~ 5 evenly at 70 ℃.

B. Warm 6 ~ 8 to 70 ℃.

C. B is added to A and emulsified.

D. C is cooled and 9 is added to obtain an ointment.

The ointment of this prescription example 6 gives moisturization to the skin, prevents the skin from drying for a long time, and has excellent moisturizing effect.

[Storage Acceptance Number]

MA KT (NITE BP-1420) Strain / Classification: Lachancea (Kluyveromyces) thermomotolerans / Storage Location: Japan Post Code 292-0818 2-5-8 Kamakami, Kisarazu, Chiba Prefecture, Independent Administration Patented Microbiological Depository Center (NPMD) of Corporation Product Evaluation Technology Base Organization / Deposit Acceptance Date: September 13, 2012 / Depositor: Maruzen Pharmaceutical Co., Ltd.

Furthermore, the aforementioned strains can be freely transferred with the approval of the depositor.

Claims (4)

A humectant, which contains the following components (A) and (B); (A) a sugar fermentation product produced by a yeast of the genus Kluyveromyces, and (B) selected from glycerin, propylene glycol, and diethylene glycol One or more polyols of propylene glycol. The humectant according to claim 1, wherein the sugar fermentation product is a sugar fermentation product derived from sugar cane. The humectant according to claim 1 or 2, wherein the dry solid content of the component (A) is 0.0001% by mass or more and 0.09% by mass or less. For example, the moisturizer of claim 1 or 2, wherein the content ratio of the dry solid content of the component (A) to the component (B) is 0.0000025 to 0.009: 1.