CN116509780B

CN116509780B - Lip essence emulsion and raw material composition, preparation method and application thereof

Info

Publication number: CN116509780B
Application number: CN202310436027.4A
Authority: CN
Inventors: 许敏; 史豆豆; 严泽民; 羌钒; 段平超
Original assignee: Huida Biomedicine Suzhou Co ltd
Current assignee: Huida Biomedicine Suzhou Co ltd
Priority date: 2023-04-21
Filing date: 2023-04-21
Publication date: 2023-10-20
Anticipated expiration: 2043-04-21
Also published as: CN116509780A

Abstract

The application discloses lip essence emulsion, a raw material composition thereof, a preparation method and application. The raw material composition of the lip essence emulsion comprises 64-85 parts of skin care agent, 4-20 parts of emollient, 1-6 parts of emulsifier, 0.1-0.5 part of antioxidant, 0.2-1 part of thickener and 0.01-0.05 part of chelating agent, and also comprises pH regulator for regulating the pH value of the system to 6-7; the skin care agent comprises 60-75 parts of rice fermentation liquor; emollients include squalane; the emulsifier comprises sodium stearyl glutamate and a nonionic compound emulsifier; the nonionic compound emulsifier comprises jojoba wax, cetyl alcohol, polyglycerol-6 distearate and polyglycerol-3 bee wax ester; the thickener comprises sodium polyacrylate. The lip essence emulsion prepared by the application has good water supplementing and retaining effects on lip skin, can improve smoothness, elasticity, fineness, glossiness and softness of the lip skin, and has high use safety and no stimulation.

Description

Lip essence emulsion and raw material composition, preparation method and application thereof

Technical Field

The application belongs to the technical field of cosmetics, and particularly relates to lip essence emulsion, a raw material composition thereof, a preparation method and application.

Background

Compared with other parts of the skin of the human body, the lip skin has larger difference, the cuticle of the lip skin is very thin and extremely sensitive, the skin is easier to dry and crack and peel, and the cheilitis is seriously caused. Lip skin care products are extremely safe and gentle relative to other facial skin care products. The prior mild lip care product has relatively weak penetration capability of active ingredients, so that the lip care product has poor moisturizing and water locking effects on the lip skin, and the elasticity of the lip skin cannot be improved.

Major technologies for promoting penetration of active ingredients in facial skin care products are of several general classes: chemically, adding penetration enhancer such as azone, alcohols, surfactant, etc.; physical methods such as ultrasound, ion, microneedle, etc.; the pharmaceutical mode is to prepare the skin care product into nanometer, liposome, etc. Because of high sensitivity of the lip skin, although the aim of effective transmission can be achieved by a common chemical method, the concentration of the penetration enhancer is larger and the irritation is high; the physical method has great side effects, such as certain stimulation and damage to skin, and may cause skin redness, swelling, pain and other uncomfortable reactions; and the cost of adopting a pharmaceutical mode is higher.

Therefore, there is a need in the art to develop a lip cream which is easily absorbed by the lip skin, has ideal moisturizing and water retention effects, and can improve the elasticity of the lip skin.

Disclosure of Invention

The application aims to solve the technical problems that the lip care product has poor moisturizing and water locking effects on lip skin and cannot improve the elasticity of the lip skin due to relatively weak penetration capability of active ingredients in the existing lip care product; the method for promoting the facial skin to absorb the active ingredients is not suitable for the lip skin or has the defects of high cost and the like, and the lip essence emulsion, the raw material composition, the preparation method and the application thereof are provided. The lip essence emulsion prepared by the application has good water supplementing and retaining effects on lip skin, can improve smoothness, elasticity, fineness, glossiness and softness of the lip skin, has high use safety, and has no stimulation to the lip skin.

The application adopts the following technical scheme to solve the technical problems:

the application provides a raw material composition of lip essence emulsion, which comprises the following components in parts by weight: 64 to 85 parts of skin care agent, 4 to 20 parts of emollient, 1 to 6 parts of emulsifier, 0.1 to 0.5 part of antioxidant, 0.2 to 1 part of thickener and 0.01 to 0.05 part of chelating agent; the raw material composition of the lip essence emulsion also comprises a pH regulator, and the pH value of the system is regulated to 6-7;

wherein the skin care agent comprises 60-75 parts of rice fermentation liquor; the rice fermentation liquor is supernatant obtained by inoculating fermentation strains in a fermentation substrate comprising rice flour, fermenting and culturing and centrifuging, wherein the fermentation strains comprise lactobacillus plantarum and/or saccharomycetes;

the emollient comprises squalane; the emulsifier comprises sodium stearyl glutamate and a nonionic compound emulsifier; the nonionic compound emulsifier comprises jojoba wax, cetyl alcohol, polyglycerol-6 distearate and polyglycerol-3 bee wax ester; the thickener comprises sodium polyacrylate.

In some embodiments, the rice fermentation broth may further comprise water in the fermentation substrate during the preparation process.

Wherein, the mass ratio of the rice flour to the water may be 1: (50-100).

In the preparation process of the rice fermentation broth of some embodiments, the grain size of the rice flour may be 0.15 to 0.3mm.

In some embodiments, the rice fermentation broth may be prepared by a process that includes sterilizing the fermentation substrate prior to use, as is conventional in the art.

Wherein the conditions and methods of sterilization may be those conventional in the art for such procedures and may generally be high temperature sterilization.

When the sterilization of the fermentation substrate is performed by the high temperature sterilization method, the sterilization temperature may be a temperature conventional in the art for such an operation, and preferably is 100 to 121 ℃.

When the sterilization of the fermentation substrate is performed by the high temperature sterilization method, the sterilization time may be a time conventional in this type of operation in the art, preferably 15 to 35 minutes, more preferably 30 minutes.

Wherein, the sterilization operation may further comprise an operation of cooling to room temperature, as is conventional in the art.

In the preparation process of the rice fermentation broth of some embodiments, the lactobacillus plantarum may include lactobacillus plantarum with a preservation number of CGMCC No.1.572 purchased from China general microbiological culture Collection center.

In the preparation process of the rice fermentation broth of some embodiments, the saccharomycete may include saccharomyces cerevisiae, preferably saccharomyces cerevisiae purchased from China general microbiological culture collection center with a collection number of CGMCC No. 2.1543.

In the preparation process of the rice fermentation broth of some embodiments, the fermentation strain can be added in the form of a fermentation strain bacterial liquid according to the conventional in the art, and the number of viable bacteria in the fermentation strain bacterial liquid per unit volume is 10 ⁶ ～10 ¹¹ CFU/mL, preferably 10 ⁷ ～10 ¹⁰ CFU/mL。

In some embodiments of the preparation of the rice fermentation broth, the number of the fermentation species inoculated per unit volume of the fermentation substrate may be conventional in the art, preferably 10 ⁵ ～10 ⁹ CFU/mL, more preferably 10 ⁶ ～10 ⁸ CFU/mL。

In the preparation process of the rice fermentation broth of some embodiments, the fermentation culture time can be 24-48 hours, preferably 24-36 hours.

In some embodiments, the temperature of the fermentation culture may be 37-43 ℃.

In the preparation process of the rice fermentation broth of some embodiments, the fermentation culture operation may further include a sterilization operation.

Wherein, the conditions and methods of the primary sterilization may be conventional in the art, and may generally be a high temperature sterilization method.

When the primary sterilization is performed by the high temperature sterilization method, the temperature of the primary sterilization may be a temperature conventional in such an operation in the art, and is preferably 100 to 121 ℃.

When the primary sterilization is performed by the high temperature sterilization method, the time for the primary sterilization may be a time conventional in such an operation in the art, preferably 15 to 35 minutes, more preferably 30 minutes.

In some embodiments, the rotational speed of the centrifugation may be conventional in this type of operation, preferably 4000 to 8000rpm, more preferably 4000 to 6000rpm, for example 4500rpm.

In some embodiments, the centrifugation may be performed for a period of time conventional in the art, preferably 10 to 40 minutes, more preferably 20 to 40 minutes, for example, 25 minutes.

In the preparation process of the rice fermentation broth of some embodiments, the centrifugation may further include a secondary sterilization operation according to the conventional method in the art.

Wherein, the secondary sterilization method can be a high temperature sterilization method conventionally used in the art.

When the secondary sterilization is performed using the high temperature sterilization method, the temperature of the secondary sterilization may be a temperature conventional in the art for such an operation, and preferably is 100 to 121 ℃.

When the secondary sterilization is performed by the high temperature sterilization method, the time for the secondary sterilization may be a time conventional in the art for such an operation, preferably 20 to 40 minutes, more preferably 30 minutes.

In some embodiments, the rice fermentation broth is preferably 64-70 parts by weight.

In some embodiments, the skin care agent may further include a water-soluble skin care agent and an oil-soluble skin care agent. The water-soluble skin care agent generally refers to an active component which is added in the skin care agent and is easy to dissolve in water and has the effect of protecting skin besides the rice fermentation liquid. The oil-soluble skin care agent generally refers to an active ingredient which is not easily dissolved in water and has skin care effect, which is additionally added in the skin care agent, besides the rice fermentation broth.

Wherein the water-soluble skin care agent may comprise at least one of dexpanthenol, dipotassium glycyrrhizinate and polypeptide skin conditioning agents.

Preferably, the dexpanthenol may be added in the form of an aqueous dexpanthenol solution, which may be 40% to 60% by mass, preferably 50% by mass, according to the conventional method in the art.

Preferably, the dipotassium glycyrrhizinate can be added in the form of dipotassium glycyrrhizinate aqueous solution according to the conventional method in the art, and the mass percentage of the dipotassium glycyrrhizinate in the dipotassium glycyrrhizinate aqueous solution can be 5% -20%, preferably 10%.

Preferably, in the polypeptide skin conditioner, the mass percentage of the polypeptide substance in the polypeptide skin conditioner can be 0.015-0.02%.

Preferably, the polypeptide skin conditioning agent comprises palmitoyl tripeptide-1 and/or palmitoyl tetrapeptide-7. When the polypeptide skin conditioning agent comprises palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7, the mass ratio of palmitoyl tripeptide-1 to palmitoyl tetrapeptide-7 may be 1: (0.5 to 1.5), preferably 1:1.

preferably, the polypeptide skin conditioner may further comprise at least one of glycerol, butylene glycol, polysorbate-20, xanthan gum and water. The glycerol, the butanediol, the polysorbate-20, and the xanthan gum may be added in amounts conventional in the art.

In a preferred embodiment, the polypeptide skin conditioner comprises 0.0075 to 0.01wt% of palmitoyl tripeptide-1, 0.0075 to 0.01wt% of palmitoyl tetrapeptide-7, 35 to 45wt% of glycerol, 15 to 25wt% of butanediol, 0.4 to 0.6wt% of polysorbate-20, 0.1 to 0.3wt% of xanthan gum and the balance water.

In a more preferred embodiment, the polypeptide skin conditioner comprises 0.01wt% palmitoyl tripeptide-1, 0.01wt% palmitoyl tetrapeptide-7, 40wt% glycerol, 20wt% butylene glycol, 0.5wt% polysorbate-20, 0.2wt% xanthan gum, and the balance water.

Preferably, the weight part of the dexpanthenol is 1-3 parts, more preferably 2 parts.

Preferably, the dipotassium glycyrrhizinate is 0.1-0.5 part by weight.

Preferably, the weight portion of the polypeptide skin conditioner is 3-5 portions.

Wherein the oil-soluble skin care agent may comprise at least one of a phytosterol-based skin conditioning agent, tocopherol, and ceramide NS/ceramide NG.

Preferably, the plant sterol skin conditioner is 0.1 to 0.5 parts by weight, preferably 0.2 parts by weight.

Preferably, the tocopherol is 0.1 to 0.5 parts by weight, more preferably 0.2 parts by weight.

Preferably, the weight part of the ceramide NS/ceramide NG is 0.01-0.05 part.

In some embodiments, the squalane may be present in an amount of 1 to 5 parts by weight, preferably 3 parts by weight.

In some embodiments, the emollient may further comprise dioctyl carbonate, C ₁₀ ～C ₁₈ At least one of fatty acid triglyceride type emollient and butter fruit fat.

Wherein, the weight part of the dioctyl carbonate can be 1-5 parts, preferably 4 parts.

Wherein the C ₁₀ ～C ₁₈ The weight fraction of the fatty acid triglyceride type emollient may be 1 to 5 parts, preferably 3.5 parts.

Wherein, the weight portion of the butter tree fruit fat can be 1-5 portions, preferably 3 portions.

In some embodiments, the weight fraction of sodium stearyl glutamate may be 0.1 to 0.5 part, preferably 0.25 to 0.3 part.

In some embodiments, the nonionic built emulsifier is Emulum of French Jia French lion ^@ Mellifera MB。

In the nonionic compound emulsifier of some embodiments, the mass ratio of jojoba wax, cetyl alcohol, polyglycerol-6 distearate and polyglycerol-3 bee wax ester may be (5-30): (3-15): (50-90): (3-15).

Wherein, the weight portion of the nonionic compound emulsifier can be 1-5 portions, preferably 3 portions.

In some embodiments, the antioxidant may include p-hydroxyacetophenone.

In some embodiments, the sodium polyacrylate may be 0.1 to 0.5 parts by weight, preferably 0.25 parts by weight.

In some embodiments, the thickener may further comprise a carbomer.

Wherein the carbomer may be 0.1 to 0.5 parts by weight, preferably 0.45 parts.

In some embodiments, the pH adjuster may comprise arginine.

In some embodiments, the chelator may include EDTA-2Na.

In some embodiments, the raw material composition of the lip cream can further comprise a humectant and/or a flavoring agent.

Wherein the weight portion of the humectant is 5 to 11.5 portions.

Wherein the humectant comprises at least one of glycerin, 1, 2-hexanediol and sodium 4D hyaluronate.

Preferably, the sodium 4D hyaluronate is a mixture of sodium hyaluronate, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate, and sodium hyaluronate cross-linked polymer, such as Hymagic manufactured by Hua Xi Biotech Inc., which is routinely used by those skilled in the art ^TM -sodium 4D hyaluronate.

Preferably, the glycerin may be 5 to 10 parts by weight, preferably 7 parts by weight.

Preferably, the weight portion of the 1, 2-hexanediol is 0.1 to 0.5 portion.

Preferably, the weight portion of the sodium 4D hyaluronate is 0.01-1 portion, preferably 0.04 portion.

Wherein the fragrance may comprise bergamot essential oil.

Wherein, the weight portion of the aromatic agent can be 0.01 to 0.05 portion.

In a preferred embodiment, the raw material composition of the lip essence emulsion comprises the following components in parts by weight: 60 to 75 parts of the rice fermentation liquor, 0.01 to 0.05 part of ceramide NS/ceramide NG, 0.1 to 0.5 part of phytosterol skin conditioning agent, 0.1 to 0.5 part of tocopherol, 1 to 3 parts of dexpanthenol, 0.1 to 0.5 part of dipotassium glycyrrhizinate, 3 to 5 parts of polypeptide skin conditioning agent, 5 to 10 parts of glycerol, 0.01 to 1 part of 4D sodium hyaluronate, 0.1 to 0.5 part of 1, 2-hexanediol, 1 to 5 parts of dioctyl carbonate and 1 to 5 parts of C ₁₀ ～C ₁₈ Fatty acid triglyceride type emollient, 1-5 parts of squalane, 1-5 parts of butter fruit, 1-5 parts of nonionic compound emulsifier, 0.1-0.5 part of sodium stearoyl glutamate, 0.1-0.5 part of p-hydroxyacetophenone, 0.1-0.5 part of carbomer, 0.1-0.5 part of sodium polyacrylate, 0.1-0.5 part of arginine and 0.01-0.05 part of EDTA-2Na.

In a more preferred embodiment, the raw material composition of the lip cream comprises the following components in parts by weight: 60 to 75 parts of the rice fermentation liquor, 0.01 to 0.05 part of ceramide NS/ceramide NG, 0.1 to 0.5 part of phytosterol skin conditioning agent, 0.1 to 0.5 part of tocopherol, 1 to 3 parts of dexpanthenol, 0.1 to 0.5 part of dipotassium glycyrrhizinate, 3 to 5 parts of polypeptide skin conditioning agent, 5 to 10 parts of glycerol, 0.01 to 1 part of 4D sodium hyaluronate, 0.1 to 0.5 part of 1, 2-hexanediol, 1 to 5 parts of dioctyl carbonate and 1 to 5 parts of C ₁₀ ～C ₁₈ Fatty acid triglyceride type emollient, 1-5 parts of squalane, 1-5 parts of butter fruit tree fruit fat, 1-5 parts of nonionic compound emulsifier, 0.1-0.5 part of sodium stearoyl glutamate, 0.1-0.5 part of p-hydroxyacetophenone, 0.1-0.5 part of carbomer, 0.1-0.5 part of sodium polyacrylate, 0.1-0.5 part of arginine, 0.01-0.05 part of EDTA-2Na and 0.01-0.05 part of aromatic.

The application also provides a preparation method of the lip essence emulsion, wherein the raw materials of the lip essence emulsion comprise the raw material composition of the lip essence emulsion, and the preparation method comprises the following steps:

(1) Mixing the rice fermentation liquor, the sodium stearyl glutamate, the antioxidant, the thickener and the chelating agent, homogenizing, and heating until the mixture is completely dissolved to obtain a material A;

(2) Uniformly mixing the emollient and the nonionic compound emulsifier to obtain a material B;

(3) Cooling the material A and the material B to 70-75 ℃, mixing, homogenizing, cooling to 55-60 ℃, adding the pH regulator to adjust the pH value of the system to 6-7, and continuously cooling to below 45 ℃;

wherein, the step (1) and the step (2) are not in sequence.

In step (1) of some embodiments, when a humectant is included in the raw material composition of the lip concentrate emulsion, the humectant is added during the mixing of step (1). The humectant is the same as the humectant in the raw material composition of the lip cream as described above.

In step (1) of some embodiments, the homogenizing conditions and methods may be conventional in the art, typically performed in a vacuum emulsification pot.

In step (1) of some embodiments, the homogenizing is performed in a vacuum emulsification pot having a homogenizer at the bottom of the vacuum emulsification pot rotating at 2500-2900 rpm.

In step (1) of some embodiments, the homogenizing is performed in a vacuum emulsification pot having a vaned stirring paddle rotating at 30-40 rpm.

In step (1) of some embodiments, the homogenized pressure may be atmospheric pressure.

In step (1) of some embodiments, the heating temperature may be conventional in the art, and may typically be 80-85 ℃.

In step (2) of some embodiments, when an oil-soluble skin care agent is included in the raw material composition of the lip concentrate emulsion, the oil-soluble skin care agent is added during the mixing of step (2). The oil-soluble skin care agent is the same as the oil-soluble skin care agent in the raw material composition of the lip concentrate emulsion as described above.

In step (2) of some embodiments, the temperature of the mixing may be conventional in the art, and may typically be in the range of 85 to 90 ℃.

In step (3) of some embodiments, the homogenizing conditions and methods may be conventional in the art, typically performed in a vacuum emulsification pot.

In step (3) of some embodiments, the homogenizing conditions and methods are the same as described above in step (1).

In step (3) of some embodiments, when a water-soluble skin care agent is included in the raw material composition of the lip concentrate emulsion, the water-soluble skin care agent is added after cooling to 45 ℃. The water-soluble skin care agent is the same as the water-soluble skin care agent in the raw material composition of the lip concentrate emulsion as described above.

In step (3) of some embodiments, the water-soluble skin care agent may be added in the form of an aqueous solution of the water-soluble skin care agent, which may be 10% to 50% by mass of the aqueous solution of the water-soluble skin care agent, as is conventional in the art.

In step (3) of some embodiments, when a fragrance is included in the raw material composition of the lip concentrate emulsion, the fragrance is added after being cooled to 45 ℃. The fragrance is the same as the fragrance in the raw material composition of the lip cream as described above.

The application also provides lip cream which is prepared by the preparation method of the lip cream.

The application also provides application of the lip essence emulsion as a raw material in preparing a lip care external agent.

On the basis of conforming to the common knowledge in the field, the above preferred conditions can be arbitrarily combined to obtain the preferred examples of the application.

The reagents and materials used in the present application are commercially available.

The application has the positive progress effects that: the application provides a lip essence emulsion which has good moisturizing and water-retaining effects on lip skin, and can improve smoothness, elasticity, fineness, glossiness and softness of the lip skin. We have developed compositions which automatically adapt and adjust to climatic and seasonal variations, which form very thin and imperceptible films in high temperature and humidity environments, rendering the product non-greasy; when the composition is in a dry and cold environment, a soft and comfortable protective film can be formed, so that the product is moistened and moisturized. The lip essence emulsion disclosed by the application can promote the absorption of skin conditioning agents such as small molecular peptides, reduce the dosage of the small molecular peptides, and reduce the product cost on the premise of achieving the same functions.

Drawings

The application may be better understood by reference to the following description taken in conjunction with the accompanying drawings. The accompanying drawings, which are included to provide a further illustration of the preferred embodiments of the application and together with a further understanding of the principles and advantages of the application, are incorporated in and constitute a part of this specification.

Wherein:

FIG. 1 is a state diagram of comparative example 4 when the temperature is lowered to 40℃with stirring in step (3).

Detailed Description

The application is further illustrated by means of the following examples, which are not intended to limit the scope of the application. The experimental methods, in which specific conditions are not noted in the following examples, were selected according to conventional methods and conditions, or according to the commercial specifications.

The experimental methods used in the following examples are conventional methods unless otherwise specified.

Example 1

The embodiment provides lip essence emulsion, wherein the raw material types and the weight parts of the components are shown in table 1;

the preparation method of the rice fermentation broth in table 1 comprises the following steps: crushing rice into rice flour with a particle size of 0.3mm, mixing 6g of rice flour with 300g of water, and sterilizing at 100 ℃ for 30min; cooling the sterilized fermentation substrate to room temperature; preparing Saccharomyces cerevisiae with collection number of CGMCC No.2.1543 from China general microbiological culture collection center with viable count concentration of 10 ⁷ Taking 10mL of the yeast liquid of CFU/mL, inoculating the yeast liquid of CFU/mL into 100mL of cooled fermentation substrate,fermenting and culturing for 24h at 40 ℃; transferring the fermented and cultured material into a high-temperature sterilizing pot for one-time sterilization, and sterilizing at 100 ℃ for 30min; centrifuging the sterilized materials at 4500rpm for 25min, collecting supernatant, transferring the supernatant into high temperature sterilizing pot for secondary sterilization, sterilizing at 100deg.C for 30min, and cooling to room temperature to obtain rice fermentation broth.

TABLE 1

The preparation method of the lip essence emulsion comprises the following steps:

(1) Uniformly mixing rice fermentation liquor, sodium stearoyl glutamate, p-hydroxyacetophenone, carbomer, sodium polyacrylate, EDTA-2Na, glycerin, 4D sodium hyaluronate and 1, 2-hexanediol, homogenizing, wherein the homogenizing pressure is normal pressure, the rotating speed of a homogenizer at the bottom of a vacuum emulsifying pot is 2500rpm, the rotating speed of a vane type stirring paddle in the vacuum emulsifying pot is 30rpm, and heating to 85 ℃ to completely dissolve materials, so as to obtain a material A;

(2) Mixing dioctyl carbonate, squalane, butter and C ₁₀ -C ₁₈ Fatty acid triglyceride type emollient and Emulium ^@ The Melifera MB charm bee emulsifier, the technical SD phytosterol, the ceramide NS/ceramide NG and the tocopherol are uniformly mixed, and the mixture is carried out in a disc disperser with the rotating speed of 500rpm and the mixing temperature of 90 ℃ to prepare a material B;

(3) Cooling the materials A and B to 70 ℃, adding the materials B into the materials A, uniformly stirring, homogenizing, wherein the homogenizing pressure is normal pressure, the rotating speed of a homogenizer at the bottom of a vacuum emulsifying pot is 2500rpm, the rotating speed of a vane type stirring paddle in the vacuum emulsifying pot is 30rpm, cooling to 55 ℃, and adding the pH value of a pre-dissolved arginine regulating system to 7; cooling the system to 40deg.C, mixing with bergamot essential oil, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, pre-dissolved aqueous solution of dexpanthenol with mass percentage of 50% and aqueous solution of dipotassium glycyrrhizinate with mass percentage of 10%, and stirring completely and uniformly.

Example 2

Compared with example 1, the lip cream was prepared with the only difference that the rice fermentation broth was different, and other condition parameters were the same as in example 1.

The preparation method of the rice fermentation broth in the embodiment comprises the following steps: crushing rice into rice flour with a particle size of 0.33mm, mixing 6g of rice flour with 300g of water, and sterilizing at 100 ℃ for 30min; cooling the sterilized fermentation substrate to room temperature; the lactobacillus plantarum with the preservation number of CGMCC No.1.572 purchased from China general microbiological culture collection center is prepared to have the viable count concentration of 10 ⁷ Inoculating 10mL of lactobacillus plantarum bacterial liquid into 100mL of cooled fermentation substrate, and fermenting and culturing for 24h at 40 ℃; transferring the fermented and cultured material into a high-temperature sterilizing pot for one-time sterilization, and sterilizing at 100 ℃ for 30min; centrifuging the sterilized materials at 4500rpm for 25min, collecting supernatant, transferring the supernatant into high temperature sterilizing pot for secondary sterilization, sterilizing at 100deg.C for 30min, and cooling to room temperature to obtain rice fermentation broth.

Comparative example 1

The preparation method of the rice fermentation broth in the embodiment comprises the following steps: crushing rice into rice flour with a particle size of 0.3mm, mixing 6g of rice flour with 300g of water, and sterilizing at 100 ℃ for 30min; cooling the sterilized fermentation substrate to room temperature; preparing lactobacillus acidophilus with collection number of CGMCC No.1.1854 from China general microbiological culture collection center into viable count concentration of 10 ⁷ CFU/mL acidophilic lactobacillus leverInoculating 10mL of lactobacillus acidophilus bacterial liquid into 100mL of cooled fermentation substrate, and fermenting and culturing for 24h at 40 ℃; transferring the fermented and cultured material into a high-temperature sterilizing pot for one-time sterilization, and sterilizing at 100 ℃ for 30min; centrifuging the sterilized materials at 4500rpm for 25min, collecting supernatant, transferring the supernatant into high temperature sterilizing pot for secondary sterilization, sterilizing at 100deg.C for 30min, and cooling to room temperature to obtain rice fermentation broth.

Comparative example 2

The difference compared with example 2 is that squalane is replaced with dioctyl carbonate in the same amount, and other conditions are the same as those in example 2, to obtain lip cream.

Comparative example 3

Compared with example 2, the difference is only that the rice fermentation broth is replaced by the deionized water with the same amount, and other condition parameters are the same as those of example 2, so as to prepare the lip cream.

Comparative example 4

In comparison with example 2, the only difference is that sodium stearoyl glutamate is replaced by an equivalent amount of Emulium ^@ Melifera MB charm bee emulsifier, other conditions parameters were the same as in example 2, to prepare a lip cream.

In the research and development process, if sodium stearyl glutamate is not added, in the step (3), after the material A and the material B are mixed, demulsification phenomenon is easy to occur, and the system is unstable.

Comparative example 5

The difference compared with example 2 is that sodium polyacrylate is replaced with xanthan gum in the same amount, and other condition parameters are the same as those in example 2, to prepare lip cream. The results show that the oil is not resistant to high temperature and oil is generated at 50 ℃ during stability test.

Comparative example 6

Compared with the example 2, the difference is only that in the step (3), the materials A and B are cooled to 80 ℃, then the material B is added into the material A, and other condition parameters are the same as the example 2, so that the lip cream is prepared.

In the research and development process, in the step (3), when the temperature of the mixture of the material A and the material B is higher than 75 ℃, the system is easy to break, and the system is unstable.

Comparative example 7

Compared with example 2, the difference is only that the nonionic compound emulsifier is replaced by equivalent amount of cetyl polyether-20, and other condition parameters are the same as those of example 2, so as to prepare the lip cream.

In the research and development process, the lip essence emulsion prepared in the comparative example 7 has inferior use feeling when being used in different seasons, and is mainly characterized by strong greasy feeling when being used in summer and difficult to be smeared uniformly when being used in winter.

Effect example 1

The moisturizing and water retention properties of the lip cream prepared in examples 1 to 2 and comparative examples 1 to 3 described above were tested with reference to the cosmetic safety specification (2015 edition) QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines.

1. Design of experiment

Test environment: temperature: 21+/-1 ℃; humidity: 50% -60%;

test area: the inner side of the forearm;

the test indexes are as follows: MMV value of skin moisture content, TEWL value of percutaneous moisture loss

Test time point: background value, 5min, 1h and 4h after sample application;

experimental instrument: MPA580 (German CK Co.) multiple probes

The testing method comprises the following steps: the test place has no direct light and no wind, the room temperature is 21-22 ℃, and the humidity is 40-60%. Any product cannot be used 2 to 3 days before the test of the tested part, and water cannot be contacted for 1 to 3 hours. The tested parts are unified before detection, and the cleaning method is to wipe the tested parts with dry facial tissues. The subject is left to stand for 30min under the constant temperature and humidity environment, and can not drink water or beverage. The moisture content and the percutaneous moisture loss were measured sequentially. The sample was measured in terms of (2.0.+ -. 0.1) mg/cm ² The amount of skin moisture and the amount of percutaneous moisture loss in the test area were collected 5min, 1h, and 4h after the sample application, respectively.

Notice that:

the tester cannot leave the working chamber during the whole test, and the test part remains exposed and uncovered.

And (5) if any allergic and uncomfortable conditions exist, immediately reporting by the testers, stopping the test, and cleaning the tested parts with a large amount of clear water.

2. Test results

2.1, skin moisture content data (results see tables 2-6)

TABLE 2 variation of skin moisture content after application of example 1 to make lip cream

TABLE 3 variation of skin moisture content after application of example 2 to make lip cream

TABLE 4 variation of skin moisture content after application of comparative example 1 to make lip cream

TABLE 5 variation of skin moisture content after application of comparative example 2 to make lip cream

TABLE 6 variation of skin moisture content after coating comparative example 3 to make lip cream

2.2, data on percutaneous Water loss (TEWL) of skin (results see tables 7-11)

TABLE 7 skin TEWL changes after application of example 1 to make lip cream

TABLE 8 skin TEWL changes after application of example 2 to make lip cream

TABLE 9 skin TEWL changes after application of comparative example 1 to make lip cream

Table 10 skin TEWL change after applying comparative example 2 to make lip cream

Table 11 skin TEWL change after applying comparative example 3 to make lip cream

3. Analysis of results

From the mean and significance analysis:

(1) The skin moisture contents of the lips essence milk prepared in example 1 are all extremely higher than the background value (P < 0.01) after 5min, 1h and 4h are all extremely lower than the background value (P < 0.05), which means that the lips essence milk prepared in example 1 has the effects of extremely remarkably improving the skin moisture content and remarkably reducing the skin moisture loss within 4h after use.

(2) The skin moisture contents of the lips essence milk prepared in example 2 are all extremely higher than the background value (P < 0.01) after 5min, 1h and 4h are all extremely lower than the background value (P < 0.01), which means that the lips essence milk prepared in example 2 has the effects of extremely remarkably improving the skin moisture content and extremely remarkably reducing the skin moisture loss within 4h after use.

(3) The skin moisture content of the lip cream prepared in comparative example 1 is extremely higher than the background value (P < 0.01) within 5min after use, the skin moisture content of the lip cream prepared in comparative example 1 is extremely higher than the background value (P < 0.05), the skin moisture content of the lip cream prepared in comparative example 1 within 1h after use is not extremely higher than the background value (P < 0.05), and the TEWL (temperature of the lip cream) prepared in 5min, 1h and 4h after use is not extremely lower than the background value (P > 0.05), which means that the lip cream prepared in comparative example 1 has the effect of remarkably improving the skin moisture content within 1h after use, but has no effect of remarkably reducing the skin moisture loss.

(4) The skin moisture content of the lip essence milk prepared in the comparative example 2 is extremely higher than the background value (P < 0.01) within 5min, 1h and 4h after the use, the TEWL is not remarkably lower than the background value (P > 0.05) within 5min and 1h after the use, and the skin TEWL is not remarkably lower than the background value (P < 0.05) within 4h after the use, which means that the lip essence milk prepared in the comparative example 2 has a certain water supplementing effect, but does not have a quick water locking effect and has high water scattering rate.

(5) The skin moisture content of the lip essence emulsion prepared in the comparative example 3 is extremely higher than the background value (P < 0.01) within 5 minutes after the use, the skin moisture content of the lip essence emulsion prepared in the comparative example 3 is extremely higher than the background value (P < 0.05), the skin moisture content of the lip essence emulsion prepared in the comparative example 3 within 1 hour after the use is not extremely higher than the background value (P < 0.05), the TEWL (temperature-induced water) of the lip essence emulsion prepared in the comparative example 3 is not extremely lower than the background value (P > 0.05) within 5 minutes after the use, and the lip essence emulsion prepared in the comparative example 3 has the effect of remarkably improving the skin moisture content within 1 hour after the use, does not have the long-term moisturizing effect and does not have the effect of remarkably reducing the skin moisture loss.

Effect example 2 Consumer trial investigation

1. Test sample

Lip cream prepared in example 2 and lip cream prepared in comparative example 3

2. Test person (age statistics see table 12, occupation statistics see table 13)

Table 12

TABLE 13

3. Content of test

The lip state before the product is used is self-evaluated, and the statistical result is shown in table 14.

TABLE 14

The habit of using other lip care products before using the product is shown in Table 15.

TABLE 15

4. Score statistics results

4.1, the two samples were scored for feel and the results are shown in Table 16.

Table 16

Options	Example 2 lip cream	Comparative example 3 lip cream
			Product appearance	8.43	8.51
Product smell	8.5	8.03
			Product consistency	8.57	8.49
Smoothness of the product when pushed open	8.5	7.07
			Skin elasticity of lips after use	8.6	6.9
After use, the lip skin is fine and smooth	8.5	6.63
			Skin gloss for lips after useDegree of gloss	8.53	6.6
Softness of lip skin after use	8.93	6.93
			The overall use effect of the product	8.63	7.03

4.2, which sample you feel more pronounced to improve lip skin elasticity, fine lines and lightening (see table 17 for results)?

TABLE 17

4.3, as consumers, you prefer to buy what product (see table 18 for results)?

TABLE 18

From the results of 30 people, the lip cream of example 2 is obviously superior to the lip cream of comparative example 3 in improving the elasticity, luster, fine feel and the like of lips.

Effect example 3

1. Comparative analysis of the system states of examples 1 to 2, comparative example 4 and comparative example 6 during the mixing and homogenizing process of step (3) of the production process is shown in table 19, the state of the lip cream prepared in comparative example 4 when the temperature of the lip cream is reduced to 40 ℃ in step (3) is shown in fig. 1, and the state of the lip cream prepared in comparative example 6 when the temperature of the lip cream is reduced to 40 ℃ in step (3) is similar to that of comparative example 4.

TABLE 19

Numbering device	System state at homogenization in step (3)
		Example 1	Stabilization
Example 2	Stabilization
		Comparative example 4	Demulsification, water-oil separation
Comparative example 6	Demulsification, water-oil separation

2. The lip cream prepared in example 1, example 2 and comparative example 5 were respectively subjected to stability tests under conditions including 50 ℃,40 ℃,25 ℃,4 ℃, -18 ℃ and product stability under light (natural light) conditions, the test period was 4 weeks, and specific test results are shown in table 20, and the results show that the lip cream prepared in comparative example 5 is subjected to oil-water separation phenomenon after being stored for 1 week at 50 ℃.

Table 20

Finally, it is also noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

While the application has been disclosed by the foregoing description of specific embodiments thereof, it will be appreciated that those skilled in the art may devise various modifications, adaptations, or equivalents of the application within the spirit and scope of the appended claims. Such modifications, improvements, or equivalents are intended to be included within the scope of this application as claimed.

Claims

1. The raw material composition of the lip essence emulsion is characterized by comprising the following components in parts by weight: 64-85 parts of skin care agent, 4-20 parts of emollient, 1-6 parts of emulsifier, 0.1-0.5 part of antioxidant, 0.2-1 part of thickener, 0.01-0.05 part of chelating agent and 5-11.5 parts of humectant; the raw material composition of the lip essence emulsion further comprises a pH regulator, and the pH value of the system is regulated to 6-7;

the skin care agent comprises 60-75 parts of rice fermentation liquor, a water-soluble skin care agent and an oil-soluble skin care agent; the water-soluble skin care agent comprises dexpanthenol, dipotassium glycyrrhizinate and polypeptide skin conditioning agents; the oil-soluble skin care agents include phytosterol-based skin conditioning agents, tocopherols, and ceramide NS/ceramide NG; the rice fermentation liquor is supernatant obtained by inoculating fermentation strains in a fermentation substrate comprising rice flour, fermenting, culturing and centrifuging, wherein the fermentation strains comprise lactobacillus plantarum with the preservation number of CGMCC No.1.572 and/or Saccharomyces cerevisiae with the preservation number of CGMCC No. 2.1543;

the humectant comprises glycerin, 1, 2-hexanediol and sodium 4D hyaluronate; the emollient comprises dioctyl carbonate, C ₁₀ ~C ₁₈ Fatty acid triglyceride emollients, shea butter and squalane; the emulsifier comprises sodium stearoyl glutamate anda nonionic type compound emulsifier; the nonionic compound emulsifier comprises jojoba wax, cetyl alcohol, polyglycerol-6 distearate and polyglycerol-3 bee wax ester; the antioxidant comprises p-hydroxyacetophenone; the thickener comprises carbomer and sodium polyacrylate.

2. The raw material composition of lip cream according to claim 1, wherein the preparation process of the rice fermentation liquid satisfies at least one of the following conditions:

the fermentation substrate further comprises water;

the grain size of the rice flour is 0.15-0.3 mm;

the fermentation substrate further comprises a sterilization operation prior to use;

the fermentation strain is added in the form of fermentation strain bacterial liquid, and the number of viable bacteria in the fermentation strain bacterial liquid per unit volume is 10 ⁶ ~10 ¹¹ CFU/mL；

The number of the fermentation strains inoculated in a unit volume of the fermentation substrate was 10 ⁵ ~10 ⁹ CFU/mL；

The fermentation culture time is 24-48 hours;

the temperature of the fermentation culture is 37-43 ℃;

the fermentation culture operation is further followed by a primary sterilization operation;

the centrifugation time is 10-40 min;

the centrifugation operation is further followed by a secondary sterilization operation.

3. The raw material composition of lip cream according to claim 2, wherein the preparation process of the rice fermentation liquid satisfies at least one of the following conditions:

the mass ratio of the rice flour to the water is 1: (50-100);

the fermentation strain is added in the form of fermentation strain bacterial liquid, and the number of viable bacteria in the fermentation strain bacterial liquid per unit volume is 10 ⁷ ~10 ¹⁰ CFU/mL；

Unit volumeThe number of the fermentation strains inoculated in the fermentation substrate is 10 ⁶ ~10 ⁸ CFU/mL；

The fermentation culture time is 24-36 hours;

the centrifugation time is 20-40 min.

4. The raw material composition of lip cream according to claim 1, wherein the raw material composition of lip cream satisfies at least one of the following conditions:

the weight part of the rice fermentation liquid is 64-70 parts;

1-5 parts of squalane;

the weight part of the dioctyl carbonate is 1-5 parts;

the C is ₁₀ ~C ₁₈ 1-5 parts of fatty acid triglyceride emollients;

the butter is prepared from 1-5 parts by weight of butter;

the weight part of the sodium stearoyl glutamate is 0.1-0.5 part;

in the nonionic compound emulsifier, the mass ratio of jojoba wax to cetyl alcohol to polyglycerol-6 distearate to polyglycerol-3 bee wax ester is (5-30): (3-15): (50-90): (3-15);

the nonionic compound emulsifier is 1-5 parts by weight;

the weight part of the sodium polyacrylate is 0.1-0.5 part;

the carbomer comprises, by weight, 0.1-0.5 part of carbomer;

the pH adjuster comprises arginine;

the chelating agent includes EDTA-2Na.

5. The raw material composition of lip cream according to claim 4, wherein the raw material composition of lip cream satisfies at least one of the following conditions:

3 parts of squalane;

and the weight part of the sodium stearoyl glutamate is 0.25-0.3 part.

6. A raw material composition of lip concentrate emulsion according to claim 1, wherein the skin care agent satisfies at least one of the following conditions:

the dexpanthenol is added in the form of a dexpanthenol aqueous solution, wherein the mass percentage of the dexpanthenol in the dexpanthenol aqueous solution is 40% -60%;

the dipotassium glycyrrhizinate is added in the form of dipotassium glycyrrhizinate aqueous solution, and the mass percentage of the dipotassium glycyrrhizinate in the dipotassium glycyrrhizinate aqueous solution is 5% -20%;

in the polypeptide skin conditioner, the mass percentage of polypeptide substances in the polypeptide skin conditioner is 0.015% -0.02%;

the polypeptide skin conditioning agent comprises palmitoyl tripeptide-1 and/or palmitoyl tetrapeptide-7;

the polypeptide skin conditioner further comprises at least one of glycerol, butanediol, polysorbate-20, xanthan gum and water;

the weight part of the dexpanthenol is 1-3 parts;

the weight part of dipotassium glycyrrhizinate is 0.1-0.5 part;

3-5 parts of polypeptide skin conditioner;

the plant sterol skin conditioner comprises, by weight, 0.1-0.5 part of plant sterol skin conditioner;

the weight part of the tocopherol is 0.1-0.5 part;

the weight part of the ceramide NS/ceramide NG is 0.01-0.05 part.

7. A raw material composition of lip cream according to claim 6, wherein when the polypeptide type skin conditioning agent comprises palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7, the mass ratio of palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 is 1: (0.5 to 1.5).

8. The raw material composition of lip cream according to claim 1, wherein the raw material composition of lip cream satisfies at least one of the following conditions:

the weight part of the glycerol is 5-10;

the weight part of the 1, 2-hexanediol is 0.1-0.5 part;

the weight portion of the 4D sodium hyaluronate is 0.01-1 portion.

9. A raw material composition of lip cream as claimed in any one of claims 1 to 8, further comprising a fragrance.

10. A raw material composition of lip cream according to claim 9, wherein the fragrance satisfies at least one of the following conditions:

the aromatic comprises bergamot essential oil;

the weight portion of the aromatic is 0.01-0.05 portion.

11. A preparation method of lip cream, which is characterized in that the raw materials of the lip cream comprise the raw material composition of the lip cream according to any one of claims 1-10, and the preparation method comprises the following steps:

(1) Mixing the rice fermentation liquor, the sodium stearyl glutamate, the antioxidant, the thickener, the chelating agent and the humectant, homogenizing, and heating to completely dissolve to obtain a material A;

(2) Uniformly mixing the oil-soluble skin care agent, the emollient and the nonionic compound emulsifier to prepare a material B;

(3) Cooling the material A and the material B to 70-75 ℃, mixing, homogenizing, cooling to 55-60 ℃, adding the pH regulator to adjust the pH value of the system to 6-7, continuously cooling to below 45 ℃, and adding the water-soluble skin care agent;

wherein, the step (1) and the step (2) are not in sequence.

12. The method for preparing lip cream according to claim 11, wherein the method satisfies at least one of the following conditions:

in the step (1), the homogenization is performed in a vacuum emulsifying pot, and the rotating speed of a homogenizer at the bottom of the vacuum emulsifying pot is 2500-2900 rpm;

in the step (1), the homogenization is performed in a vacuum emulsifying pot, and the rotating speed of a vane type stirring paddle in the vacuum emulsifying pot is 30-40 rpm;

in the step (1), the homogenizing pressure is normal pressure;

in the step (1), the heating temperature is 80-85 ℃;

in the step (2), the mixing temperature is 85-90 ℃;

in the step (3), the water-soluble skin care agent is added in the form of a water-soluble skin care agent aqueous solution, wherein the water-soluble skin care agent accounts for 10% -50% of the water-soluble skin care agent aqueous solution in percentage by mass;

in the step (3), when a flavoring agent is included in the raw material composition of the lip concentrate emulsion, the flavoring agent is added after being cooled to 45 ℃;

in the step (3), the homogenization is performed in a vacuum emulsifying pot, and the rotating speed of a homogenizer at the bottom of the vacuum emulsifying pot is 2500-2900 rpm;

in the step (3), the homogenization is performed in a vacuum emulsifying pot, and the rotating speed of a vane type stirring paddle in the vacuum emulsifying pot is 30-40 rpm;

in the step (3), the homogenizing pressure is normal pressure.

13. A lip cream as defined in claim 11 or 12, which is prepared by a process for preparing a lip cream.

14. Use of the lip cream according to claim 13 as a raw material for preparing a lip care external preparation.