CN112999143B

CN112999143B - Repairing and relieving skin care product containing metazoan components and preparation method thereof

Info

Publication number: CN112999143B
Application number: CN202110231026.7A
Authority: CN
Inventors: 王习振; 王领; 王荣川
Original assignee: Shandong Aiyidian Biotechnology Co ltd
Current assignee: Qingdao Yuanda Biotechnology Co ltd
Priority date: 2021-03-02
Filing date: 2021-03-02
Publication date: 2022-06-24
Anticipated expiration: 2041-03-02
Also published as: CN112999143A

Abstract

The invention provides a repairing and relieving skin care product containing metazoan components and a preparation method thereof, wherein the repairing and relieving skin care product contains the following components in percentage by mass: 1-5% of post-prebiotics component, 1-8% of grease, 3-5% of skin conditioner, 9-15% of humectant, 0.5-8% of surfactant, 0.6-1.2% of thickener and the balance of deionized water. The metazoan components comprise antibacterial substances and active metabolites, have the effects of rebuilding skin cells, improving elastin and updating metabolism of the dermis layer, and have the effect of promoting the proliferation of cells of the stratum corneum, and the prepared skin care product can enhance the elasticity of skin and has obvious effects of relieving and repairing skin. The post-prebiotics component is a mixed solution obtained by fermenting a plurality of strains of lactobacillus plantarum, lactobacillus casei, bifidobacterium lactis, bifidobacterium animalis and lactobacillus rhamnosus; compared with the metabiotic component obtained by single strain fermentation, the effects of eliminating wrinkles and enhancing skin elasticity are better, and the problem of multidimensional skin can be solved.

Description

Repairing and relieving skin care product containing metazoan components and preparation method thereof

Technical Field

The invention relates to the technical field of cosmetics, in particular to a repairing and relieving skin care product containing metazoan components and a preparation method thereof.

Background

The skin has mainly a triple barrier: stratum corneum barrier, microecological barrier and immune barrier, and secondly, skin microecology, which is the subject of current enthusiastic research in the dermatology community, have been increasingly studied for the treatment of skin diseases (atopic dermatitis, acne, rosacea, dandruff, chloasma, sensitive skin, etc.) by correcting the dysbiosis. For example, acne problems and Propionibacterium acnes hyperproliferation have a positive correlation, dandruff is associated with abnormal proliferation of Malassezia, and drugs used to treat these skin problems often have a mechanism of action that inhibits the excessive proliferation of certain harmful bacteria. Therefore, the skin state stability and the skin micro-ecological balance have important relation.

Under normal conditions, skin microorganisms, the external environment and a host are in interaction in dynamic balance, the ecological balance of the skin is maintained, the skin is stimulated when the ecological balance of the facial skin is broken, a series of problems are aggravated or derived if the skin cannot be repaired in time, inflammation is further generated, color spot aging, seborrheic dermatitis, acne, folliculitis and the like are caused, allergic dermatitis can be caused when staphylococcus aureus excessively occupies the surface of the skin, and skin pruritus and stabbing pain are caused. The key point of nursing microecological balance lies in balance, the proportion of any kind of bacteria is not increased, the use of postbiotic bacteria avoids the problems of low bioavailability and unstable effect of the probiotic viable bacteria, the capacity of inhibiting lipid peroxidation and scavenging free radicals for skin cells is obviously improved by adding postbiotic filtrate generated by fermentation and metabolism of various probiotic viable bacteria, and the skin barrier is repaired by reasonably selecting bacteria in a multi-dimensional manner to enhance the skin immunity. The metazoan is different from prebiotics, can directly generate beneficial effect on skin, and refers to inactivated thallus components or metabolites of specific strains in the in vitro culture process.

Chinese patent document CN 110772433A (201911120976.1) is a compound multiple post-growth vitality-activating essence, which comprises the following components in percentage by weight: 8.0-13.0% of butanediol, 2.0-3.0% of caprylic acid/capric acid/succinic acid triglyceride, 2.0-3.0% of polymonosodium glutamate, 0.5-1.0% of Japanese ardisia herb extract, 0.5-1.0% of yeast/rice fermentation product filtrate, 0.5-1.0% of fission yeast mycelium fermentation product filtrate, 0.5-1.0% of lactobacillus/mung bean seed extract/sodium glutamate fermentation product filtrate, 0.5-1.0% of lactobacillus fermentation product, 0.5-1.0% of bacillus/rice bran extract/soybean extract fermentation product filtrate, 0.5-1.0% of streptococcus thermophilus fermentation product, 0.1-0.2% of colloidal silver and 65.3-79.4% of deionized water; the essence is placed in a sealed container, and hydrogen is injected into the essence. The patent can achieve the effect of repairing skin, and the active matter combination injected with hydrogen has prominent effects of reducing the amount of allergic erythema and reducing the recurrence of allergic fading and redness.

However, the operation technology of the patent steps is more complicated, the effect of the needed metazoan ingredients is single, multiple kinds of metazoan ingredients are needed to be matched for use, purchasing factors are more limited, and the existing skin care product containing metazoan has no obvious effects on preventing and eliminating skin wrinkles, resisting inflammation, improving skin elasticity and the like.

Disclosure of Invention

The invention provides a repairing and relieving skin care product containing postbiotic components and a preparation method thereof, aiming at improving the performances of moisturizing and tendering skin, enhancing skin elasticity and repairing and relieving skin of the skin care product.

In order to achieve the purpose, the invention adopts the following technical scheme:

a repairing and relieving skin care product containing metazoan components is characterized by comprising the following components in percentage by mass: 1-5% of post-prebiotics component, 1-8% of grease, 3-5% of skin conditioner, 9-15% of humectant, 0.5-8% of surfactant, 0.6-1.2% of thickener and the balance of deionized water.

If the addition amount of the postbiotic component is less than 1%, the compounding effect is not large, and the postbiotic component cannot be completely fused with other components; if the addition amount of the postbiotic component is more than 5%, the anti-sensitivity of the postbiotic component after compounding is reduced to a certain extent. In skin care products, the prebiotic component also acts primarily as a skin conditioner.

Preferably, the postbiotic ingredients include an antibacterial substance and an active metabolite;

the antibacterial substance at least comprises the following components in concentration:

500-550 mu g/g of lactic acid, 1880-1950 mu g/g of citric acid, 95-105 mu g/g of malic acid, 28-32 mu g/g of phenyllactic acid, 14.5-15 mu g/g of succinic acid and 21-22 mu g/g of 4-hydroxyphenyllactic acid;

the active metabolites include at least the following concentrations of components:

4100-4200 mu g/g of lauryl diethanolamine, 280-290 mu g/g of soyasaponin, 250-260 mu g/g of soybean isoflavone, 210-220 mu g/g of malonyl genistin, 190-195 mu g/g of 3-indoleacrylic acid, 210-215 mu g/g of genistein, 85-90 mu g/g of hyaluronic acid, 345-355 mu g/g of exopolysaccharide, 850-860 mu g/g of polypeptide, 810-820 mu g/g of irone and 350-360 mu g/g of Preaustinol A.

Preferably, the antibacterial substance further comprises 0.1-0.2 mu g/g of salicylic acid, 0.5-1 mu g/g of vanillic acid, 0.2-0.25 mu g/g of phenylpropionic acid and 0.05-0.10 mu g/g of tartaric acid.

Preferably, the active metabolites further comprise 270-275 mu g/g of isostearic acid, 230-240 mu g/g of trihydroxyflavone, 210-215 mu g/g of daidzein and 175-180 mu g/g of butyrin.

The concentration of each component of the metazoan refers to the content of the component in the metazoan.

Preferably, the post-biotic component further comprises short chain fatty acids, the short chain fatty acids comprising at least the following components in concentrations:

60-65 mug/g of acetic acid, 7-7.5 mug/g of propionic acid, 11-12 mug/g of butyric acid, 2-2.5 mug/g of valeric acid and 2.5-3 mug/g of caproic acid.

Preferably, the metazoan component is a mixed solution obtained by fermenting a plurality of strains of lactobacillus plantarum, lactobacillus casei, bifidobacterium lactis, bifidobacterium animalis and lactobacillus rhamnosus. Compared with the metabiotic component obtained by single strain fermentation, the effects of eliminating wrinkles and enhancing skin elasticity are better, and the problem of multidimensional skin can be solved.

Preferably, the humectant is at least one selected from glycerol, butanediol, betaine, glyceryl polyether-26, 1, 3-propanediol, 1, 2-hexanediol and panthenol; further preferably, the humectant is one or more of glycerol, butanediol, glyceryl polyether-26, betaine and 1, 2-hexanediol.

Further preferably, the content of the glycerol in the skin care product is 3-5%; the content of betaine in the skin care product is 2-3%; the content of butanediol in the skin care product is 3-4%; the content of the 1, 2-hexanediol in the skin care product is 0.48-0.52%; the content of the glyceryl polyether-26 in the skin care product is 0-2%.

Preferably, the oil and fat is selected from at least one of squalane, jojoba oil, shea butter, avocado oil, nut oil, glyceryl caprylate/caprate, coco alcohol-caprylate/caprate, dimethyl siloxane, cyclopentasiloxane; further preferably, the grease is selected from one or more of squalane, coco-caprylate/caprate, jojoba oil and polydimethylsiloxane; more preferably, the oil is squalane, coconut oil alcohol-caprylate/caprate and jojoba oil, and the mass ratio of the squalane, the coconut oil alcohol-caprylate/caprate and the jojoba oil is 1-2: 1: 1.

Preferably, the skin conditioner is at least one of oat beta-glucan, yogurt extract, bisabolol, centella asiatica extract, calendula extract, caprylhydroxamic acid, and p-hydroxyacetophenone. Further preferably, the skin conditioner comprises beta-glucan from oat, yogurt extract, marigold extract, lemon extract, p-hydroxyacetophenone. Further preferably, the lemon extract is also included.

Further preferably, the content of the oat beta-glucan in the skin care product is 1.8-2.2%; the content of the yogurt extract is 0.3-0.7%; the content of the p-hydroxybenzophenone is 0.48-0.52%; the content of the calendula extract is 0.4-0.5%; the content of the lemon extract is 0-0.5%.

The oat beta-glucan is a non-starch polysaccharide existing in oat cell walls and is obtained by enzymolysis and extraction, and the oat beta-glucan has a sun-screening effect and can reduce inflammation, promote Langerhans cell proliferation and enhance skin immunity. The yogurt extract is prepared by fermenting and extracting lactobacillus bulgaricus and thermophilic bacillus strains, and mainly contains extracellular polysaccharide generated by lactobacillus in the yogurt fermentation process, so that the yogurt extract has the effects of eliminating color spots and brightening skin. The p-hydroxyacetophenone is a natural plant extract, is present in stems and leaves of artemisia scoparia belonging to the family Compositae, artemisia capillaris, plants belonging to the family Roxaceae, roots of vines belonging to the family Panax, and the like, is a preservative promoter, and has multiple effects of oxidation resistance, irritation resistance and the like.

Preferably, the surfactant is at least one of cetearyl glucoside, cetearyl alcohol, glyceryl stearate, sucrose stearate and sorbitan olive oleate; further preferably, the surfactant is one or two of cetearyl glucoside and cetearyl alcohol.

More preferably, the content of the cetearyl glucoside in the skin care product is 0.1-2%; the content of cetearyl alcohol in the skin care product is 0-4%.

Preferably, the thickener is at least one of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, acryloyl dimethyl taurate/VP copolymer, hydroxyethyl acrylate/acryloyl dimethyl taurate copolymer, xanthan gum and hydroxyethyl cellulose; further preferably, the thickener comprises one or two of acryloyl dimethyl taurate/VP copolymer and hydroxyethyl acrylate/acryloyl dimethyl taurate copolymer.

Further preferably, the content of the acryloyl dimethyl taurate/VP copolymer in the skin care product is 0-1%; the content of the hydroxyethyl acrylate/acryloyl dimethyl sodium taurate copolymer in the skin care product is 0.2-0.8%.

The invention also provides a preparation method of the repairing and relieving skin care product containing the metazoan component, which is characterized by comprising the following steps:

(1) mixing the humectant, the thickener and deionized water, heating to 80-85 ℃, and continuously stirring to fully and uniformly stir all the components to obtain a water-phase material;

(2) mixing the grease and the surfactant, heating to 75-85 ℃, and continuously stirring to fully and uniformly stir all the components to obtain an oil phase material;

(3) adding the water-phase material into emulsifying equipment, adding the oil-phase material while stirring, and homogenizing for 3-7 minutes; the homogenization temperature is controlled to be 75-80 ℃;

(4) after homogenizing, preserving heat for 20 minutes for sterilization, starting cooling water, keeping the temperature of the cooling water at 25 ℃, carrying out homogenizing mechanical stirring and simultaneously vacuumizing, and defoaming for 10 minutes;

(5) cooling to 45 deg.C, adding the prebiotics component and skin conditioner, and stirring to mix the components uniformly;

(6) cooling to 30-35 ℃, and discharging to obtain cream-like substances.

Preferably, the emulsifying device in the step (3) is an emulsifying pot.

The invention also provides application of the skin care product as an effective component in cosmetics such as sleeping masks, lotions and creams.

The invention has at least the following beneficial effects:

the selected post-biotic component is a mixed solution obtained by fermenting a plurality of strains of lactobacillus plantarum, lactobacillus casei, bifidobacterium lactis, bifidobacterium animalis and lactobacillus rhamnosus; compared with the metabiotic component obtained by single strain fermentation, the effects of eliminating wrinkles and enhancing skin elasticity are better, and the problem of multidimensional skin can be solved.

The selected multiple antibacterial substances and active metabolites have the effects of reconstructing skin cells, improving elastin and updating metabolism of a dermis layer, can reduce aging signs, has a promotion effect on the proliferation of cells of the stratum corneum, relieves damaged skin, is beneficial to the overall health of the skin, and is compounded with multiple other components with multiple effects of moisture retention, allergy resistance, repair and the like to achieve the purposes of synergy, slow release and long acting.

The yoghurt extract, the calendula extract and the prebiotics component are compounded, so that the yoghurt extract, the calendula extract and the prebiotics component have a synergistic effect, the skin moisturizing and inflammation diminishing effects are improved, and the repairing effect is better. Through the compound use of a plurality of components, the bioavailability of the metazoan components is improved, the skin care product prepared by the formula can enhance the skin elasticity, obviously repair and relieve the skin, and the skin tolerance test is safe and non-irritant.

Drawings

FIG. 1 is a skin occlusion patch test presentation;

FIG. 2 is a graph of different skin indicators over time;

FIG. 3 shows the improvement of the 28-day face sensitive red zone;

FIG. 4 is a graph of wrinkle and skin elasticity as a function of time;

fig. 5 shows the improvement of wrinkles on the surface of a patient using 28 days.

Detailed Description

The technical solutions of the present invention are further described below with reference to the following examples, but the scope of the present invention is not limited thereto.

The metazoan components used in the embodiment of the invention are purchased from different batches of products of the bioscience and technology Limited company of Jinhua Yinhe, Zhejiang, and are detected to be in accordance with content indexes, and can be purchased from the unit by the technical personnel in the field for implementing the invention.

The yogurt extract, the split yeast fermentation lysate, and the lactobacillus fermentation product used in the specific examples of the present invention were obtained from the anglida biotechnology, ltd, texas. The raw materials used in the examples of the present invention were all common commercial products unless otherwise specified.

Example 1

A repairing and relieving skin care product containing metazoan components comprises the following components in percentage by mass:

postbiotic ingredients: 1.0 percent;

detecting the post-biotic product to obtain the following specific contents of the components:

an antibacterial substance: 501 mu g/g of lactic acid, 1882 mu g/g of citric acid, 95.3 mu g/g of malic acid, 28.1 mu g/g of phenyllactic acid, 14.5 mu g/g of succinic acid, 21.3 mu g/g of 4-hydroxyphenyllactic acid, 0.11 mu g/g of salicylic acid, 0.56 mu g/g of vanillic acid, 0.22 mu g/g of phenylpropionic acid and 0.05 mu g/g of tartaric acid;

short chain fatty acids: acetic acid 60 mug/g, propionic acid 7.1 mug/g, butyric acid 11.2 mug/g, valeric acid 2.1 mug/g, caproic acid 2.5 mug/g;

active metabolite (b): lauryl diethanol amine 4168 mu g/g, soyasaponin 285 mu g/g, soybean isoflavone 252 mu g/g, malonyl genistin 211 mu g/g, 3-indoleacrylic acid 192 mu g/g, genistein 214 mu g/g, hyaluronic acid 85.7 mu g/g, exopolysaccharide 355 mu g/g, polypeptide 850.5 mu g/g, irone 812 mu g/g, Preaustinol A358.2 mu g/g, isostearic acid 275 mu g/g, trihydroxyflavone 230 mu g/g, daidzein 212 mu g/g, and glyceryl butyrate 177 mu g/g.

Skin conditioner: 2% of oat beta-glucan, 0.5% of yoghurt extract, 0.5% of p-hydroxyacetophenone and 0.6% of calendula extract.

Humectant: 3% of glycerol, 2% of betaine, 3% of butanediol, 1% of glyceryl polyether-26 and 0.5% of 1, 2-hexanediol.

Surfactant (b): 0.1% cetearyl glucoside, 3.5% cetearyl alcohol.

Grease: 0.5% coco-caprylate/caprate, 0.7% squalane, 0.5% jojoba oil.

Thickening agent: 0.4% ammonium acryloyldimethyltaurate/VP copolymer, 0.2% hydroxyethyl acrylate/sodium acryloyldimethyltaurate copolymer.

The balance being deionized water.

The preparation method of the repairing and relieving skin care product containing the metazoan component comprises the following steps:

(1) mixing the humectant, the thickener and deionized water, heating to 80 ℃ and continuously stirring to fully and uniformly stir all the components to obtain a water phase material;

(2) mixing the grease and the surfactant, heating to 75 ℃, and continuously stirring to fully and uniformly stir all the components to obtain an oil phase material;

(3) adding the water phase material into an emulsifying device, adding the oil phase material while stirring, and homogenizing for 3 minutes; the homogenization temperature is kept at 75 ℃;

(4) after homogenizing, preserving heat for 20 minutes for sterilization, starting cooling water (the temperature of the cooling water is 25 ℃ at normal temperature), stirring by a homogenizer while vacuumizing, and defoaming for 10 minutes;

(5) cooling to 45 deg.C, adding the prebiotics components and various skin conditioners, and stirring to mix the components uniformly;

(6) cooling to 30 deg.C, and discharging to obtain cream product.

Example 2

postbiotic ingredients: 3.0 percent of the total weight of the mixture,

the antibacterial substance comprises 550 mu g/g of lactic acid, 1948 mu g/g of citric acid, 105 mu g/g of malic acid, 31.2 mu g/g of phenyllactic acid, 15 mu g/g of succinic acid, 21.8 mu g/g of 4-hydroxyphenyllactic acid, 0.17 mu g/g of salicylic acid, 0.52 mu g/g of vanillic acid, 0.25 mu g/g of phenylpropionic acid and 0.09 mu g/g of tartaric acid;

the short chain fatty acid comprises acetic acid 65 μ g/g, propionic acid 7.5 μ g/g, butyric acid 11.5 μ g/g, valeric acid 2.3 μ g/g, caproic acid 2.8 μ g/g;

the active metabolites comprise 4107 mu g/g of lauryl diethanol amine, 2802 mu g/g of soyasaponin, 260 mu g/g of soybean isoflavone, 219 mu g/g of malonyl genistin, 195 mu g/g of 3-indoleacrylic acid, 210 mu g/g of genistein, 90 mu g/g of hyaluronic acid, 345 mu g/g of extracellular polysaccharide, 860 mu g/g of polypeptide, 818 mu g/g of irone, 352 mu g/g of Preaustenitid A, 270 mu g/g of isostearic acid, 240 mu g/g of trihydroxyflavone, 215 mu g/g of daidzein and 178 mu g/g of butyrin.

Skin conditioner: 2% oat beta-glucan, 0.7% yoghurt extract, 0.5% p-hydroxyacetophenone, 0.4% calendula extract;

humectant: 5% glycerol, 3% betaine, 3% butanediol, 0.5% 1, 2-hexanediol;

surfactant (B): 0.2% cetearyl glucoside, 4% cetearyl alcohol;

grease: 2% coco-caprylate/caprate, 4% squalane, 2% jojoba oil;

thickening agent: 0.9% of an ammonium acryloyl dimethyl taurate/VP copolymer, 0.3% of a hydroxyethyl acrylate/sodium acryloyl dimethyl taurate copolymer;

the balance being deionized water.

(1) mixing the humectant, the thickener and deionized water, heating to 85 ℃ and continuously stirring to fully and uniformly stir all the components to obtain a water phase material;

(2) mixing oil and a surfactant, heating to 80 ℃, and continuously stirring to fully and uniformly stir all components to obtain an oil phase material;

(3) adding the water phase material into an emulsifying device, adding the oil phase material while stirring, and homogenizing for 7 minutes; the homogenizing temperature is controlled at 80 ℃;

(4) after homogenizing, preserving heat for 20 minutes for sterilization, starting cooling water (the temperature of the cooling water is 25 ℃ at normal temperature), stirring by a homogenizer, vacuumizing, and defoaming for 10 minutes;

(6) cooling to 35 deg.C, and discharging to obtain cream product.

Example 3

postbiotic ingredients: 5.0 percent of the total weight of the mixture,

the antibacterial substance comprises 530 mu g/g of lactic acid, 1910 mu g/g of citric acid, 99 mu g/g of malic acid, 30 mu g/g of phenyllactic acid, 14.7 mu g/g of succinic acid, 21.5 mu g/g of 4-hydroxy phenyllactic acid, 0.15 mu g/g of salicylic acid, 0.68 mu g/g of vanillic acid, 0.24 mu g/g of phenylpropionic acid and 0.08 mu g/g of tartaric acid;

the short chain fatty acid comprises 62 mug/g of acetic acid, 74 mug/g of propionic acid, 11.5 mug/g of butyric acid, 2.3 mug/g of valeric acid and 3 mug/g of caproic acid;

the active metabolites comprise lauryl diethanol amine 4128 mu g/g, soyasaponin 289 mu g/g, soybean isoflavone 256 mu g/g, malonyl genistin 217 mu g/g, 3-indoleacrylic acid 193 mu g/g, genistein 214 mu g/g, hyaluronic acid 88 mu g/g, exopolysaccharide 347 mu g/g, polypeptide 855 mu g/g, irone 815 mu g/g, Preaustinol A360 mu g/g, isostearic acid 272 mu g/g, trihydroxyflavone 235 mu g/g, daidzein 213 mu g/g, and glyceryl butyrate 179 mu g/g.

Skin conditioner: 2% oat beta-glucan, 0.3% yoghurt extract, 0.5% p-hydroxyacetophenone, 0.5% calendula extract, 0.5% lemon extract;

humectant: 5% glycerol, 3% betaine; 4% of butanediol, 2% of glyceryl polyether-26 and 0.5% of 1, 2-hexanediol;

surfactant (B): 0.5% cetearyl glucoside;

grease: 1.5% coco-caprylate/caprate, 2.5% squalane, 1.5% jojoba oil;

thickening agent: 0.8% hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer;

the balance being deionized water.

(3) adding the water-phase material into an emulsifying device, adding the oil-phase material while stirring, and homogenizing for 7 minutes; the homogenizing temperature is controlled at 80 ℃;

(6) cooling to 35 deg.C, making the product into cream, and discharging.

In the embodiment, 0.5% of lemon extract is added, so that the product has special natural fragrance without adding essence while the skin care effect is improved.

Comparative example 1

A skin care product comprises the following components in percentage by mass:

skin conditioner: 2% oat beta glucan, 0.5% yoghurt extract, 0.6% calendula extract, 0.5% p-hydroxyacetophenone, 1.5% yeast bifida ferment lysate; the lysate of the yeast fermentation product of the bifidus is a metabolite, a cytoplasm fragment, a cell wall component and a polysaccharide complex obtained by culturing, inactivating and decomposing the bifidobacterium, and the component has strong anti-immunosuppression activity and can promote DNA repair and other effects.

Humectant: 3% of glycerol and 2% of betaine; 3% of butanediol, 1% of glyceryl polyether-26 and 0.5% of 1, 2-hexanediol;

surfactant (B): 0.1% cetearyl glucoside, 3.5% cetearyl alcohol;

grease: 0.5% coco-caprylate/caprate, 0.7% squalane, 0.5% jojoba oil;

thickening agent: 0.4% of an ammonium acryloyldimethyltaurate/VP copolymer, 0.2% of a hydroxyethyl acrylate/sodium acryloyldimethyltaurate copolymer;

the balance being deionized water.

The preparation method of the repairing and soothing skin care product of comparative example 1 comprises the following steps:

(3) adding the water phase material into an emulsifying device, adding the oil phase material while stirring, and homogenizing for 7 minutes; the homogenizing temperature is controlled to be 75-80 ℃;

(4) after homogenizing, preserving heat for 20 minutes for sterilization, starting cooling water (normal temperature 25 ℃), stirring by a homogenizer, vacuumizing, and defoaming for 10 minutes;

(6) cooling to 35 deg.C, making the product into cream, and discharging.

Comparative example 2

A skin care product comprises the following components in percentage by mass:

skin conditioner: 2% oat beta-glucan, 0.5% yoghurt extract, 0.6% calendula extract, 0.5% p-hydroxyacetophenone; 1.0% two split yeast fermentation product lysate, 1% lactobacillus fermentation product;

the lysate of the fermentation product of the yeast bifidus is a metabolite obtained by culturing, inactivating and decomposing the bifidobacterium, the component has strong immunosuppressive activity and can promote the DNA repair and other effects, nisin in the fermentation product of the lactobacillus has broad-spectrum antibacterial property, has an inhibiting effect on gram-negative bacteria and gram-positive bacteria, enhances the immunity, resists inflammation and relieves the allergic dermatitis.

surfactant (b): 0.1% cetearyl glucoside, 3.5% cetearyl alcohol;

grease: 0.5% coco-caprylate/caprate, 0.7% squalane, 0.5% jojoba oil;

the balance being deionized water.

The preparation method of the repairing and soothing skin care product of the comparative example 2 comprises the following steps:

(2) mixing the grease and the surfactant, heating to 80 ℃, and continuously stirring to fully and uniformly stir all the components to obtain an oil phase material;

(3) adding the water-phase material into an emulsifying device, adding the oil-phase material while stirring, and homogenizing for 7 minutes; the homogenizing temperature is controlled at 75 ℃;

(6) cooling to 35 deg.C, making the product into cream, and discharging.

Comparative example 3

A skin care product and a preparation method thereof comprise the following components by mass percent:

the other is the same as example 1 except that the skin conditioner: 2% of oat beta-glucan, 1.1% of yoghurt extract and 0.5% of p-hydroxyacetophenone.

Comparative example 4

the other examples are the same as example 1 except that the skin conditioning agents are: 2% of oat beta-glucan, 0.5% of p-hydroxyacetophenone and 1.1% of calendula extract.

1. And (3) safety testing:

to better illustrate the safety of the product of the invention, the cosmetic products described above were subjected to the following tests.

The quoted standards are: 2015 edition of technical Specification for cosmetic safety, GB 17149.1-1997 diagnosis standards and treatment principles for skin diseases of cosmetics, and GB 17149.2-1997 diagnosis standards and treatment principles for contact dermatitis of cosmetics.

The test process comprises the following steps:

the sample numbers of the backs of all the spotters are marked, and the products of example 1, example 2, example 3, comparative example 1, comparative example 2, comparative example 3 and comparative example 4 are added in equal amount, the control group is a blank group without any substance, and the forearm curveal side skin of the volunteer is selected as a tested part. Adding 0.02g of tested product into each patch button; control wells were blank without any material. The plaque test device with the test substance and the blank control is applied to the curved side of the forearm of the subject by using a low-sensitivity adhesive tape, and is lightly pressed by the palm to be uniformly applied to the skin for 24 hours.

Skin reactions were observed 30min, 24h and 48h after removal of the test article plaque test device, respectively. Skin reactions were observed according to the criteria of table 1 and the observations were recorded. If necessary, follow-up visits can be increased and follow-up in positive subjects should continue until the response is less than "+ +".

And (4) judging the standard:

TABLE 1 skin closed Patch test skin response grading Standard

Results and analysis:

TABLE 2 skin occlusion Patch test results

As can be seen from the table 2 and the attached figure 1, the results of the human skin closed patch experiments show that 0 of the cosmetics prepared in the examples 1-3 has positive reaction, and the tested substances in the groups do not cause adverse reaction on the skin of the tested subjects in the batch.

2. Moisture retention and efficacy testing: to better illustrate the effectiveness of the product of the present invention, the moisture retention and efficacy of the cosmetic product obtained by the present invention were tested and analyzed.

The quoted standards are: the cosmetic moisturizing efficacy evaluation guideline QB/T4256 plus 2011, the cosmetic influence test method through epidermis water loss T/ZHCA 003 plus 2018 and the internal test standard of the institution.

The implementation scheme is as follows:

screening was performed by the investigator, meeting enrollment and exclusion criteria, and after signing the volunteer protocol book, the test was attended, similar efficacy cosmetics were discontinued 48h before the test began, and any other activities affecting the screening assessments were performed.

Subjects met inclusion criteria and were enrolled in the study. Similar efficacy cosmetics are not used for 48 hours before the test is started, and on the test day, the test part is cleaned by clear water, and data collection is carried out after the test part is statically seated for 30min in a constant temperature and humidity environment (the temperature is 21 +/-1 ℃ and the relative humidity is 50% +/-10% RH). Collecting facial skin moisture content, transdermal moisture loss, skin elasticity, skin hemoglobin and facial VISIA image, taking data, filling in consumer questionnaire, and distributing sample.

The using method comprises the following steps: after cleaning the face before sleeping, 5g of the facial mask is uniformly smeared on the face for standing for 15-20 minutes, and then the redundant facial mask is wiped off. The sample wafers of example 1, comparative example 2, comparative example 3 and comparative example 4 were selected for efficacy test in consideration of feasibility factors such as product safety, effect and economy, the service cycle was 4 weeks, and skin test was performed according to the return visit cycle. Skin tests and visits were made on day 0, 14, 28, respectively, and the detailed test procedure was as described above.

Target number of examples:

the test subjects were scheduled to enroll 21 persons per test product, ensuring that the final test of each sample was completed by 21 persons.

Testing indexes are as follows:

water content of skin stratum corneum: the test was performed 3 times using Corneometer CM825 and the average was taken.

Parameter interpretation: the moisture content of the skin can influence the formation of a water and grease mixed film on the surface of the skin, the barrier function of the skin can be directly influenced, and the larger the measured value is, the higher the moisture content of the skin is.

Transdermal water dispersion loss: the measurements were performed 3 times using a TewameterTM300 and averaged.

Parameter interpretation: the transdermal water loss is generally related to the skin barrier, and a larger value indicates a greater skin water loss, a lower water-locking effect, and a poorer skin barrier.

Facial VISIA image analysis software: VISIA was used to photograph the left, middle and right sides of the face for image analysis.

Parameter interpretation: and analyzing the index data score, wherein the improvement is better when the score is smaller.

Skin elasticity: the skin elasticity R2, Q1 index was tested using a Cutometer MPA 580.

Parameter interpretation: the smaller the measurement value, the poorer the skin elasticity is.

Skin heme test: skin care products can be evaluated for improvement in skin inflammation using mexametrmx 18 to test for skin hemoglobin.

Parameter interpretation: the larger the measurement, the larger the amount of skin erythema and the more severe the inflammation.

And (3) data analysis:

the SPSS software is used to make descriptive statistics on various measurements, including quantity, mean, standard deviation, etc.

Performing pairing T test on all index data, analyzing whether the skin parameters have significant differences before and after using the product, and adopting a significance labeling method: "n.s" indicates no statistical difference, P > 0.05; "+" indicates significant difference, P is more than or equal to 0.01 and less than 0.05; "+" indicates significant difference, 0.001 ≦ P < 0.01, "+" indicates very significant difference, P < 0.001.

Results and analysis

Results of skin repair test

The skin data of the volunteers are successfully collected through VISIA facial images, MexametrMX 18 probes, Comeometer CM825 and TewameterTM300, and the effects of repairing and tendering the skin of the test sample are analyzed through four indexes.

Table 3 example 1 comparative analysis of skin indices at different times for samples (X ± s, n ═ 21)

Table 4 example 1 sample significant comparison analysis of skin indicators at different periods

Note: significance notation method "n.s" indicates no statistical difference, P > 0.05; "+" indicates significant difference, P is more than or equal to 0.01 and less than 0.05; "+" indicates significant difference, 0.001 ≦ P < 0.01, "+" indicates very significant difference, P < 0.001.

The test results for the sample of example 1 were analyzed as follows:

the change in water content of the stratum corneum over time during the test period is shown in table 3, table 4 and fig. 2 a. The moisture content of the skin can influence the formation of a water and grease mixed film on the surface of the skin, can directly influence the barrier function of the skin and participate in the pathogenesis of aged skin and sensitive skin. The subjects showed an upward trend in facial moisture content throughout the test period, with a significant difference of P ≦ 0.001 at 14D and a 28.90% increase in the average gloss value at 28D, indicating that the sample of test example 1 was significantly effective in increasing skin moisture content.

The change in facial transdermal water dispersion over time during the test period is shown in table 3, table 4 and figure 2 b. Compared with the background value, the face cheek roughness shows a remarkable descending trend at 14D and 28D, and the descending degree is maximum at 14D; on day 28, the mean value of the percutaneous water dispersion was reduced by 36.44%. Statistical significance analysis shows that p is less than or equal to 0.001 (shown in Table 2) at day 14, and the difference is very significant.

The change in the red zone data measured by VISIA during the test period is shown in tables 3 and 4, fig. 2c and fig. 3, the red zone reflects the condition of capillary vessels, generally associated with skin inflammation and impaired stratum corneum skin barrier, and the lower score indicates a healthier skin barrier. The improvement in the red zone was significant by 18.28% over the 28 day time range using the sample of example 1. Statistical significance analysis shows that the significance difference is obvious when P is more than or equal to 0.01 and less than 0.05 at 14D and 28D (shown in Table 2).

The time course of hemoglobin during the test period is shown in Table 3, Table 4 and FIG. 2 d. The hemoglobin can be used for analyzing skin erythema change before and after skin care product. The smaller the heme value, the less inflammatory erythema of the skin and the better the skin barrier status. After the sample of example 1 is used, the skin heme shows a remarkable reduction trend in the 28-day time range of the experimental period, and the average value of the heme is reduced by 12.33% (P is more than or equal to 0.001 and less than 0.01) on the 28 th day, and the remarkable difference is remarkable.

In the same test evaluation method, the samples of comparative examples 1 to 4 were tested for 28-day change rate% of skin index, and the results are shown in table 5:

TABLE 5 28-day Change in skin index (%). of comparative examples 1 to 4

The results of example 1 and comparative examples 1,2 illustrate that: from the change of water content, the change of heme, the change of red area and the change rate of water loss, the improvement effect (moisturizing, red fading and inflammation diminishing repair) of the skin of the embodiment 1 is far higher than that of the comparative example 1 and the comparative example 2, and the red fading and inflammation diminishing repair effect is far better than that of the comparative example 1 and the comparative example 2. The reason is that the selected metazoan component in the embodiment 1 has good ion resistance and wide applicable pH range, and a small amount of low-cost components are added for compounding, so that a good skin improvement effect can be achieved. Compared with the commonly used skin conditioner ingredient, the post-biotic ingredient of the invention is the yeast fermentation lysate of fission yeast, and has the advantages of low cost and good effect.

As can be seen from the example 1 and the comparative examples 3 and 4, the yogurt extract, the calendula extract and the metazoan component are compounded and added into the formula together, so that the synergistic effect is achieved, the skin moisturizing effect is better, the moisturizing performance is improved, and the red fading, inflammation diminishing and repairing effects are better.

The VISIA facial image analysis system and the MPA580 elastic measuring probe are used for smoothly collecting the skin data of the volunteer and analyzing indexes to verify the effects of the product in the aspects of improving the skin elasticity and reducing wrinkles.

Table 6 example 1 comparative analysis of skin elasticity and wrinkles at various times (X ± s, n ═ 21)

TABLE 7 comparative analysis of skin wrinkle and elasticity significance at different periods of time

Note: significance notation method "n.s" indicates no statistical difference, P > 0.05; "+" indicates significant difference, P is more than or equal to 0.01 and less than 0.05; "x" indicates significant difference, 0.001 ≦ P < 0.01, and "x" indicates very significant difference, P < 0.001.

The test results for the sample of example 1 were analyzed as follows:

the facial wrinkle measurements during the test period are shown in table 6, table 7, fig. 4a and fig. 5. Compared with the initial value, the wrinkles show a descending trend, the average value of the facial wrinkles is reduced by 11.77% on the 28 th day, and the statistical analysis shows that P is more than or equal to 0.01 and less than 0.05, and the remarkable difference is obvious. As can be seen in fig. 5, the fine lines are significantly reduced in the test subjects after the example 1 sample is used, indicating that the example 1 sample has some effect in ameliorating wrinkles, especially fine lines.

The results of the skin elasticity measurements during the test period are shown in table 6, table 7 and figure 4 b. The R2 and Q1 values tended to rise during the test period compared to the initial values. Higher R2 and Q1 values indicate better skin elasticity. When the elastic R2 index is used to 28D, the average value of the elastic R2 index is improved by 16.07 percent (P is more than or equal to 0.01 and less than 0.05), and the obvious difference is achieved.

TABLE 8 comparative examples 1-4 sample skin elasticity and 28 day wrinkle Change Rate%

	Skin wrinkles change%	Skin elasticity R2 Change%	Rate of change in skin elasticity Q1%
				Comparative example 1	-7.36％	9.28％	9.23％
Comparative example 2	-7.8％	9.17％	9.09％
				Comparative example 3	-6.2％	5.42％	8.25％
Comparative example 4	-3.6％	3.86％	6.36％

From the results of example 1 and comparative examples 1 and 2, it can be seen that: example 1 is shown by skin wrinkle change, skin elasticity R2 change and skin elasticity Q1 change rate, which are all much higher than those of comparative example 1 and comparative example 2, namely, the wrinkle removal effect and the skin elasticity enhancing effect of example 1 are much better than those of comparative example 1 and comparative example 2. The single strain fermentation product can not solve the problem of multidimensional skin and has limited exertion effect.

As can be seen from the example 1 and the comparative examples 3 and 4, the yogurt extract, the calendula extract and the metazoan component are compounded and added into the formula together, so that the synergistic effect can be achieved, the coarse lines are changed into fine lines, the facial wrinkles are changed more obviously, the skin elasticity is improved, and the skin aging is delayed, which shows that the efficacy after compounding is superior to that of a single component.

Through testing on the moisture content of the stratum corneum, the transdermal water dispersion amount, the heme content, the skin elasticity and the facial VISIA analysis system, the sample in the test example 1 has different degrees of improvement effects on volunteers in a test period, so that skin wrinkles and inflammations are reduced, the elasticity and the moisture content are increased, and the test sample has certain effects of repairing, relieving and tendering the skin.

As can be seen from the analysis of the water content of the facial cuticle and the transdermal water dispersion loss data, the water content of the cuticle is in an increasing trend and the transdermal water dispersion loss is in a decreasing trend compared with the initial value in the testing period. The test example 1 shows that the moisture content of stratum corneum is obviously increased and the transdermal water loss is obviously reduced after the sample is used, and the test sample has certain effects of repairing and tendering skin.

The analysis of the heme data and the skin red zone data shows that the skin heme content and the red zone show obvious descending trend compared with the initial value in the test period. After the test example 1 sample is used, the content of heme and the red area index are obviously reduced, which indicates that the test sample has a certain relieving and repairing effect on skin.

Through VISIA facial image analysis and skin elasticity numerical analysis, facial wrinkles are in a descending trend and skin elasticity is in an ascending trend compared with an initial value in a test period. After the sample of the test example 1 is used, fine lines of the volunteers are obviously reduced, and the elasticity of the skin is increased, which shows that the test sample has certain effects of improving the elasticity of the skin, reducing wrinkles and delaying aging.

Subjective self-evaluation results:

table 9 example 1 sample effective self-evaluation of improvement in different skin conditions after 28 days of use

As can be seen from table 9, the effective rate of improvement of skin-lightening and capillary-reducing effect was 95.2% after 28 days using the sample of test example 1; the effective rate of improving the darkness, the gloss, the smoothness and the moistening and moisture-keeping degree is 85.7 percent, 95.2 percent, 90.5 percent and 95.2 percent respectively; the effective rate of improvement of moisturization was considered to be 95.2%, the effective rate of improvement of wrinkles was considered to be 95.2%, and the effective rate of the subject for the overall use feeling of the product was 61.9%.

After the samples of comparative example 1 and comparative example 2 are used for 28 days, the effective rate of improving the skin-lightening and fine hair-shrinking effects is 76.2 percent and 71.4 percent respectively; the effective rate of improving the darkness, the gloss, the smoothness and the moisture retention is respectively 66.7 percent, 71.4 percent, 47.6 percent, 66.7 percent, 71.4 percent, 76.2 percent, 66.7 percent and 76.2 percent; the effective rate of improving water retention was considered to be 66.7% and 71.4%, respectively, the effective rate of improving wrinkles was considered to be 76.2% and 66.7%, and the effective rate of the subject for the overall use feeling of the product was considered to be 16.7% and 18.9%.

After the samples of the comparative example 3 and the comparative example 4 are used for 28 days, the effective rate of improving the skin brightening and fine hair hole shrinking effects is 71.4 percent and 66.7 percent respectively; the effective rates of improving the darkness, the luster, the smoothness and the moistening degree are 71.4 percent, 47.6 percent, 66.7 percent, 57.1 percent, 47.6 percent, 57.1 percent, 66.7 percent and 71.4 percent respectively; the effective rate of improving the water retention was 71.4% and 66.7%, respectively, the effective rate of improving wrinkles was 57.1% and 47.6%, and the effective rate of the subjects for the overall use feeling of the product was 23.8% and 14.3%.

The foregoing invention has been described in some detail by way of illustration and example for purposes of illustration and understanding. It will be obvious to a person skilled in the art that the inventive content may be varied and modified within the scope of the appended claims. Accordingly, it is to be understood that the above description is intended to be illustrative, and not restrictive.

Claims

1. The repairing and relieving skin care product containing the metazoan component is characterized by comprising the following components in percentage by mass: 1-5% of post-prebiotics component, 1-8% of grease, 3-5% of skin conditioner, 9-15% of humectant, 0.5-8% of surfactant, 0.6-1.2% of thickener and the balance of deionized water;

the postbiotic component consists of an antibacterial substance, an active metabolite and a short-chain fatty acid;

the antibacterial substance comprises the following components:

500-550 mug/g of lactic acid, 1880-1950 mug/g of citric acid, 95-105 mug/g of malic acid, 28-32 mug/g of phenyllactic acid, 14.5-15 mug/g of succinic acid, 21-22 mug/g of 4-hydroxyphenyllactic acid, 0.1-0.2 mug/g of salicylic acid, 0.5-1 mug/g of vanillic acid, 0.2-0.25 mug/g of phenylpropionic acid and 0.05-0.10 mug/g of tartaric acid;

the active metabolite consists of the following components:

4100-4200 mug/g of lauryl diethanolamine, 280-290 mug/g of soyasaponin, 250-260 mug/g of soybean isoflavone, 210-220 mug/g of malonyl genistin, 190-195 mug/g of 3-indoleacrylic acid, 210-215 mug/g of genistein, 85-90 mug/g of hyaluronic acid, 345-355 mug/g of extracellular polysaccharide, 850-860 mug/g of polypeptide, 810-820 mug/g of irone, 350-360 mug/g of Preaustinoid A, 270-275 mug/g of isostearic acid, 230-240 mug/g of trihydroxyflavone, 210-215 mug/g of soybean, 175-180 mug/g of butyrin;

the short-chain fatty acid consists of the following components:

acetic acid is 60-65 mug/g, propionic acid is 7-7.5 mug/g, butyric acid is 11-12 mug/g, valeric acid is 2-2.5 mug/g, and caproic acid is 2.5-3 mug/g;

the oil is at least one selected from squalane, jojoba oil, shea butter, avocado oil, nut oil, glyceryl caprylate/caprate, cocoyl alcohol-caprylate/caprate, dimethyl siloxane, and cyclopentasiloxane;

the skin conditioner is at least one of oat beta-glucan, sour milk extract, bisabolol, centella asiatica extract, calendula extract, caprylyl hydroximic acid and p-hydroxyacetophenone;

the humectant is at least one selected from glycerol, butanediol, betaine, glyceryl polyether-26, 1, 3-propylene glycol, 1, 2-hexanediol and panthenol;

the surfactant is at least one of cetearyl glucoside, cetearyl alcohol, glycerol stearate, sucrose stearate and sorbitan olivate;

the thickening agent is at least one of acryloyl dimethyl ammonium taurate/VP copolymer, hydroxyethyl acrylate/acryloyl dimethyl sodium taurate copolymer, xanthan gum and hydroxyethyl cellulose.

2. The rejuvenating and soothing skin care product according to claim 1 wherein said moisturizing agent is one or more of glycerin, butylene glycol, glyceryl polyether-26, betaine, 1, 2-hexanediol.

3. The repair and soothing skin care product containing an metagenic ingredient as claimed in claim 1, wherein said oil is selected from one or more of squalane, coco-caprylate/caprate, jojoba oil, and dimethicone.

4. The repairing and relieving skin care product containing the metagenic component as claimed in claim 3, wherein the oil is squalane, coco oil-caprylate/caprate, jojoba oil in a mass ratio of 1-2: 1: 1.

5. The restorative, soothing, and skin care product of claim 1 containing post-biotic ingredients, wherein the skin conditioning agent comprises oat beta-glucan, yogurt extract, calendula extract, p-hydroxyacetophenone.

6. The restorative, soothing, and skin-care product of claim 5, wherein the skin conditioner further comprises lemon extract.

7. The restorative soothing skin care product containing post-biotic ingredients as claimed in claim 1, wherein the surfactant is one or both of cetearyl glucoside and cetearyl alcohol.

8. The restorative soothing skin care product containing postbiotic ingredients as in claim 1, wherein the thickener comprises one or both of an ammonium acryloyl dimethyl taurate/VP copolymer and a hydroxyethyl acrylate/sodium acryloyl dimethyl taurate copolymer.

9. A method of preparing a restorative soothing skin care product containing post-biotic ingredients as defined in any of claims 1 to 8, comprising the steps of:

(1) mixing the humectant, the thickener and deionized water, heating to 80-85 ℃, and continuously stirring to fully and uniformly stir the components to obtain a water-phase material;

(3) adding the water-phase material into emulsification equipment, adding the oil-phase material while stirring, and homogenizing for 3-7 minutes; the homogenizing temperature is controlled to be 75-80 ℃;

(4) after homogenization, preserving heat for 20 minutes for sterilization, starting cooling water, wherein the temperature of the cooling water is 25 ℃, stirring by a homogenizer and simultaneously vacuumizing, and defoaming for 10 minutes;

(5) cooling to 45 deg.C, adding prebiotics component and skin conditioner, and stirring to mix the components uniformly;

(6) cooling to 30-35 ℃, and discharging to obtain cream-like substances.

10. Use of the after-growth ingredient-containing rejuvenating and soothing skin care product according to any one of claims 1 to 8 or the skin care product prepared by the method according to claim 9 for preparing cosmetics such as sleeping masks, lotions, creams and the like.