TW201302243A - Skin-improving agent for oral intake - Google Patents

Abstract

To provide a skin-improving agent for oral intake, a collagen production promoter for oral intake, and a food product with which collagen production capability can be promoted by oral intake and which is highly safe in that it is natural and can be continuously taken orally. The invention is a skin-improving agent for oral intake the purpose of which is to improve skin firmness, smoothness, wrinkles, and laugh lines, which is characterized in comprising the plant or extract of lingonberry (Vaccinium vitis-idaea) and alum (Phyllantus emblica or Emblica officinale); a collagen production promoter for oral intake, which is characterized in comprising the plant or extract of lingonberry and alum; and a food product characterized in comprising the plant or extract of lingonberry and alum.

Description

Oral ingestion skin improver

The present invention relates to a skin improving agent for oral ingestion of a plant body or an extract thereof containing Vaccinium vitis-idaea and Phyllanthus emblica or Emblica officinale, or a collagen production promoter for oral ingestion, And food and beverage products.

In recent years, as an annoyance of women's beauty, there has been a problem that wrinkles or slack of the skin increase with age. Further, it is known that wrinkles or slacks of such skin are caused by a decrease in collagen production ability of skin fibroblasts.

Therefore, in order to promote the production of collagen, an external preparation for skin containing various plant extracts has been developed (Patent Document 1). In the external preparation for skin, the active ingredient is percutaneously absorbed and applied to fibroblasts. The difference in active ingredients sometimes causes problems in the absorption of the skin, and may not necessarily achieve sufficient effects.

On the other hand, in the case of oral ingestion like food, it is directly ingested into the body, so that the active ingredient is easily absorbed compared with the application of the external method, and a sufficient effect can be expected.

A collagen production promoter containing an extract of Phyllanthus emblica is known as a food capable of improving collagen production (Patent Document 2), and the industry is expected to further promote collagen by adding other components. Produces and improves the firmness or smoothness of the skin not only on the face but on the whole body, but also improves the skin with wrinkles or striated lines that are not noticeable. A higher skin improving agent for oral intake.

In addition, the extract of bilberry (alias moss) contains arbutin, which acts on tyrosinase to inhibit the synthesis of melanin, so it is well known that it is formulated into foods and the like to provide a whitening effect. (Patent Document 3), but such an extract of cranberry (alias moss) has not been known to have an effect related to collagen production ability.

[Patent Document 1] Japanese Patent Laid-Open Publication No. 2006-160629

[Patent Document 2] Japanese Patent Laid-Open Publication No. 2008-156287

[Patent Document 3] Japanese Patent Laid-Open Publication No. 2010-111590

An object of the present invention is to provide a natural and safe oral ingestion skin which is excellent in the effect of promoting the production of collagen by oral ingestion and improving the firmness of the skin. A improving agent, a collagen production promoting agent for oral ingestion, and a food or drink.

The inventors of the present invention conducted intensive studies to solve the above problems, and as a result, found that Vaccinium vitis-idaea and Euphorbiaceae are continuously ingested by the Ericaceae (Vaccinium) of the Ericaceae family. The plant body of the genus Phyllantus (Emblicahus emblica or Emblica officinale) or its extract can promote the production of collagen, thereby showing the firmness or smoothness of the skin, wrinkles or decrees. The present invention is completed by the fact that the texture becomes inconspicuous and the like, and the remarkable effect of the skin is improved.

That is, the present invention is a skin improving agent for oral ingestion, which aims to improve skin firmness, smoothness, wrinkles and striated lines, and is characterized in that 于: A plant body containing bilberry and amla, or an extract thereof.

Further, the present invention relates to a collagen production promoter for oral ingestion, which comprises a plant body containing cranberry and emblica or an extract thereof.

Moreover, the present invention relates to a food or drink comprising the above-mentioned orally ingested skin improving agent or orally ingesting collagen production promoting agent.

According to the skin improving agent for oral ingestion of the present invention, when the oral ingestion is continued, the amount of collagen is increased, and the elasticity of the skin is increased, thereby improving the firmness or smoothness of the skin throughout the body, wrinkles or striated lines. It becomes less obvious, etc., which can improve skin aging caused by age.

In addition, the skin improving agent for oral ingestion of the present invention contains an edible natural plant or an extract thereof as an active ingredient, and thus has high safety and can be continuously ingested, and can be set according to various preferences. The form at the time of ingestion can be used as a food or drink or an oral medicine.

The skin improving agent for oral ingestion of the present invention contains Vaccinium vitis-idaea of the genus Vaccinium which can be orally ingested, and yam of the genus Emblica of the genus Eucalyptus (Emblica) A plant body obtained by Phyllanthus emblica or Emblica officinale or an extract thereof is used as an active ingredient.

Vaccinium vitis-idaea, also known as moss, grows naturally in the Arctic Arctic Circle or Canada and is known to be rich in vitamin C or citric acid. The fruit can be eaten raw and is often used in processed foods such as juices and jams.

The portion of the bilberry used in the present invention is not particularly limited, and The fruit (immature fruit, mature fruit, dried fruit), leaves, roots, flowers, seeds, and the like are used arbitrarily. Among them, it is preferred to use a fruit.

The bilberry used in the present invention may be directly used as a fresh bilberry, or may be dried. However, in terms of usability, formulation, and the like, it is preferably used as a dry powder or a solvent extract.

As a method of obtaining a dry powder, various parts of a plant (fruit, leaves, roots, flowers, seeds, etc.) or whole plants are finely cut or pulverized, followed by drying, or the plants are dried, finely cut or pulverized. A method of obtaining a dry powder. Further, a method in which the plant is finely cut or pulverized, subjected to fermentation or enzyme treatment, dried, and pulverized as necessary to obtain a specific particle diameter may be suitably used.

The extract of bilberry can be obtained according to a usual method. For example, it can be obtained by immersing bilberry as needed, immersing it in an extraction solvent for a fixed period of time, or contacting with an extraction solvent heated under reflux, followed by filtration and concentration. The extraction solvent may be used arbitrarily as long as it is a solvent used for the usual extraction, and may be used alone or in combination, for example, an alcohol such as water, methanol, ethanol, propylene glycol, 1,3-butylene glycol or glycerin; chloroform; An organic solvent such as dichloroethane, carbon tetrachloride, acetone or ethyl acetate. The extract obtained by the above solvent extraction may be used as it is, or the concentrated extract may be used, or the extract may be obtained by an adsorption method, for example, using an ion exchange resin to remove impurities, or by using a porous polymer (for example, The column of Amberlite XAD-2) is adsorbed, and then dissolved and concentrated by methanol or ethanol. Further, an extract obtained by, for example, extraction with water/ethyl acetate by a distribution method, or the like can also be used. Bilberry extract as for example "LINGONBERRY EXTRACT" (manufactured by ORYZA OIL & FAT CHEMICAL Co., Ltd.) is commercially available, and these commercially available products can also be used.

The Phyllanthus emblica or Emblica officinale used in the present invention belongs to the deciduous sub-arbor of the genus Emblica of the genus Eucalyptus, which is distributed from India to Malaysia and southern China, and is generally considered to be the origin of India. Yu Ganzi has its own name according to the local language or language. For example, it can also be called: Yuganzi, citrus, Amalaka, Pokok Melaka, Indian gooseberry, etc. .

The use site of the Phyllanthus emblica is not particularly limited, and the fruit (immature fruit, mature fruit, dried fruit), leaves, roots, flowers, seeds, and the like can be used arbitrarily. Among them, the fruit is preferably used.

Phyllanthus emblica can be used directly as a fresh yam, or can be dried, but it is preferably used as a dry powder or a solvent extract in terms of usability, formulation, and the like. The method for obtaining a dry powder or an extract is not particularly limited, and the method described in the above bilberry can be employed. The Phyllanthus emblica extract is commercially available, for example, from Sun Chemical Co., Ltd. as "SunAmla", and the like, and these commercially available products can also be used.

The skin improving agent for oral ingestion of the present invention, as described in detail in the examples, enhances the elasticity of the skin by promoting the collagen production ability in the fibroblast, and as a result, the firmness or smoothness of the skin is improved. Wrinkles or striated lines become inconspicuous and inhibit the skin aging caused by age.

The skin improving agent for oral ingestion and the collagen production promoting agent for oral ingestion of the present invention can be incorporated into various foods and drinks, and can be ingested in the form of a food for improving the skin, and can also be administered in the form of a pharmaceutical preparation.

Moreover, the food or drink of the present invention can be obtained by blending a plant body of Bilberry and Phyllanthus emblica or an extract thereof in various foods and drinks, but in the mass production step, the above-mentioned oral skin improving agent or the like can also be used. A semi-finished product is prepared by using a collagen production accelerating agent for oral ingestion, and the semi-finished product is blended in various foods and drinks, thereby efficiently obtaining a food or drink having a skin-improving effect.

The amount of the bilberry and the yoghurt to be ingested (administered) can be appropriately determined according to the method of ingestion (administration) or the dosage form, and the like, and it is preferable to set the amount of ingestion (administration) per day as follows. Preparation: Bilberry is 0.1 mg~10 g, preferably 1~1000 mg, especially 10~100 mg, and 0.1 mg~10 g, preferably 1~1000 mg, especially in dry mass. Good for 10~100 mg.

When the skin improving agent for oral ingestion of the present invention or the collagen production promoter for oral ingestion is formulated in a food or drink, the additive may be arbitrarily selected and used as needed. As an additive, a functional material, an excipient, and a flavoring agent may be included.

Examples of the functional material include various vitamins such as pantothenic acid, folic acid, and biotin; various minerals such as zinc, calcium, magnesium, and iron; amino acids, oligosaccharides, propolis, royal jelly, ginkgo biloba, turmeric, and EPA ( Eicosapentaenoic acid, eicosapentaenoic acid), DHA (Docosahexaenoic acid, docosahexaenoic acid), coenzyme Q10, chondroitin, lactic acid bacteria, lactoferrin, isoflavones, prunes, chitin, polyglucosamine Glucosamine, α-lipoic acid, Brazilian mushroom (Agaricus), Garcinia Cambogia, propolis, collagen, astaxanthin, ForsLean, catechin, sesamin, ceramide, Cochorus olitorius, Spirulina ), Cat's Claw, etc. These functional materials may be used singly or in combination of two or more.

The excipients are usually used as long as they are prepared into a desired dosage form, and examples thereof include starches such as wheat starch, rice starch, corn starch, potato starch, dextrin, and cyclodextrin; and crystalline cellulose. Types; sugars such as lactose, glucose, sucrose, reduced maltose, sucrose, fructooligosaccharides, emulsified oligosaccharides; sugar alcohols such as sorbitol, erythritol, xylitol, lactitol, and mannitol. These excipients may be used singly or in combination of two or more.

Examples of the flavoring agent include various juice extracts such as a denier extract, a lychee extract, and a grapefruit extract; various juices such as apple juice, orange juice, and lemon juice; various flavors such as peach flavor, plum flavor, and yoghurt flavor; Various sweeteners such as potassium sulfamate, sucralose, erythritol, oligosaccharides, mannose, xylitol, isomerized sugars; various sour agents such as citric acid, malic acid, tartaric acid, gluconic acid; Various tea ingredients such as oolong tea, Banaba tea, Eucommia tea, Tieguanyin tea, scented tea, safflower tea, scented white tea, and kelp tea. These flavoring agents may be used singly or in combination of two or more.

In addition, a coloring agent, a preservative, a tackifier, a binder, a disintegrating agent, a dispersing agent, a stabilizer, a gelling agent, an antioxidant, a surfactant, a preservative, a pH adjusting agent, etc. may be appropriately selected from foods and the like. It is used by a publicly used person.

The form of the food or drink can be arbitrarily selected, for example, in the form of a liquid, a solid, a granule, a granule, a paste, or a gel.

Specific examples of foods and drinks include juices, vegetable juices, refreshing drinks, and beverages such as tea; soups, puddings, yoghurts, cake premixes, cakes, biscuits, candies, soft candy, chewing gum, and the like. Various kinds of ordinary processed foods; nutritional supplement foods such as beverages, designated foods for health care, functional foods, and healthy foods and drinks. However, it is not limited to these examples.

When the orally ingestible skin improving agent or the orally ingestible collagen production promoting agent of the present invention is used in the form of a pharmaceutical preparation, the dosage form can be appropriately selected, and for example, it can be used as a tablet, a granule, a powder, or a capsule. a solid preparation, or a liquid preparation such as an internal liquid preparation or a syrup preparation, etc., in which the usual use of a binding agent, a disintegrating agent, a viscosity increasing agent, a dispersing agent, and a resorption promoting agent may be appropriately used. Excipients such as flavoring agents, buffering agents, surfactants, dissolution aids, preservatives, emulsifiers, isotonic agents, stabilizers or pH adjusters.

[Examples]

Hereinafter, the test and the results performed to confirm the effects of the present invention will be described in more detail, but the present invention is not limited to the following test examples or examples.

Test 1 "Determination of collagen production ability" (experiment method)

1. Human dermal fibroblasts were seeded into 24-well plates and cultured continuously in DMEM medium containing 10% FBS.

2. After confirming that it was in a fused state, it was replaced with 0.5 mL of DMEM culture solution containing each sample, and cultured at 37 ° C for 72 hours.

3. After the culture, the collagen concentration in the culture solution was measured by an enzyme-linked immunosorbent assay (Enzyme-linked immunosorbent assay).

(treatment liquid)

Sample 1: Reference

Sample 2: Phyllanthus 10 μg/mL

Sample 3: Bilberry 10 μg/mL

Sample 4: bilberry 5 μg/mL + emblica 5 μg/mL

Yuganzi uses "SunAmla" (trade name) manufactured by Sun Chemical Co., Ltd. Further, the extract having the same effect (the extract of Phyllanthus emblica) can be obtained by extracting the fruit as a site of use using a polar solvent such as hot water or water.

"The LINGONBERRY EXTRACT" (trade name) manufactured by ORYZA OIL & FAT CHEMICAL Co., Ltd. is used for the bilberry. Further, the extract (Bilberry extract) having the same effect can be obtained by extracting a fruit as a site of use using a polar solvent such as hot water or ethanol.

In order to verify whether there is a difference between the groups based on the collected samples, statistical processing is performed. If the test results show a significance probability (p) of 5% (p < 0.05), it is considered that there is a significant difference between the data items (called variables) used in the test. If p is less than 10% (p < 0.1), there is a significant tendency difference between the data items (called variables) used in the test. Producing significant difference and significant propensity to indicate that for some reason Make a difference. When conducting a biological test, whether there is a significant difference in the end is a method of whether there is a change between the derived groups. Statistical analysis was carried out using the Tukey method.

Fig. 1 is a graph showing the results of measurement of the collagen production ability of the above samples 1 to 4. From the results of Fig. 1, it was confirmed that Sample 4 (with bilberry and Phyllanthus emblica) was significantly inferior to collagen 1 (control) in terms of collagen production ability (p < 0.001). Further, it was confirmed that Sample 4 (containing cranberry and emblica) was inferior to collagen 2 (p<0.10) in comparison with Sample 2 (with melon alone). According to this, it was confirmed that the skin improving agent of the present invention is excellent in the effect of increasing collagen production by multiplying the effect of cranberry and emblica.

Then, the test was carried out by a functional inspector, and the effects of the present invention were confirmed by the tests shown below. Four samples were prepared, and each sample was subjected to a continuous administration test by 15 functional inspectors. The functional inspector took the test sample (beverage) for one week by oral ingestion for one week.

The formulation of the test sample (beverage) is shown in Table 1.

Test 2 "Determination of the viscoelasticity of the skin" (experiment method)

The skin viscoelasticity of the back skin of the functional inspector was measured using a Cutometer (manufactured by Courage+Khazaka Co., Ltd.) after the continuous administration test (after 8 weeks of administration).

The Cutometer is a machine for evaluating changes in the skin after being attracted under a fixed pressure, and the data shown in Fig. 2 can be obtained. The values of R0 to R8 and F3 shown in the figure indicate the following physical properties, respectively.

R0: easy to stretch (better for full stretching) The bigger the value, the better)

R1: Ease of recovery to the original position (it is better to return to the original position) Smaller numbers are better)

R3: easy to stretch (preferably full stretch) The bigger the number, the better)

R8 (R0-R1): Evaluation of the effect of easy stretching and easy recovery The bigger the value, the better

F3: the area between the above envelope maximum value (Envelope max) and the minimum value (min) curve (equivalent to the accumulation of R8) The bigger the value, the better

Figures 3 to 6 are graphs showing the results of the measurement of the viscoelasticity of the skin. The skin of Group D (Bilberry + Phyllanthus emblica) had excellent viscoelastic effect, and a significant difference was confirmed on the viscoelasticity R0, R3, R8 and F3 with respect to Group A (placebo). Further, in the D group (Bilberry + Phyllanthus emblica), a significant difference (p < 0.05) was observed on R8 with respect to the C group (Bilberry), and a tendency difference (p < 0.10) was confirmed on R0, R3, and F3. In this way, it is confirmed that the skin improving agent of the present invention is excellent in the effect of improving the elasticity of the skin and improving the firmness of the skin.

Test 3 "Evaluation of wrinkles around the eyes" (experiment method)

Take a picture of the corner of the eye after the start of the continuous test and 8 weeks after the start of the continuous test (the end of the test). Photographs were taken from photographs at each time point by a dermatologist using a wrinkle rating standard photograph and scored on a 1/4 value score.

From the scores before and after taking, the wrinkle improvement degree was calculated by the formula of "wrinkle improvement degree" = "score after sample taking" - "score before sample taking". Furthermore, regarding the difference between groups, a U-test of Mann-Whitney was carried out.

<The standard of visual judgment of wrinkles>

Visual review using a standard photo of wrinkle grade (8 grades of wrinkle grade 0 to 7) (Japan Cosmetics Society, Guidelines for Cosmetic Function Evaluation) The price is scored by a score of 1/4 (for example, 3.25, 3.5, etc.). Wrinkle determination is performed by a dermatologist.

"Score: Benchmark"

Level 0: no wrinkles

Level 1: Confirm the slight wrinkles that are not obvious

Level 2: Confirm a slightly lighter wrinkle

Level 3: Obviously noticeable shallow wrinkles

Level 4: Apparently slightly wrinkles in the apparently shallow wrinkles

Level 5: Confirm a deep wrinkle

Level 6: Obvious and deep wrinkles confirmed

Level 7: Confirmation of very deep wrinkles

Fig. 7 is a view showing the result of wrinkle improvement. It was confirmed that the wrinkle improvement effect of the skin of the D group (Bilberry + Phyllanthus emblica) was excellent, and there was a significant difference (P<0.01) with respect to the A group (placebo). Further, it was confirmed that the D group (Bilberry + Phyllanthus emblica) had a tendency to be inferior to the C group (Bilberry) (p < 0.10). Thus, it was confirmed that the skin improving agent of the present invention is excellent in the effect of improving wrinkles of the skin.

Trial 4 "Questionnaire Evaluation" (experiment method)

On the start date of the continuous use test and after 1 day, 3 days, 7 days, 14 days, 28 days, and 56 days after the start of the test, the "significant degree of the decree", "the apparent degree of the forehead wrinkles", "the smoothness of the skin" Conduct a questionnaire survey.

The questionnaire was evaluated by VAS (short for visual analogue scale) which is a general evaluation scale. In other words, on each survey day, the tester was asked to record a line of paper that was drawn as a straight line of 10 cm. The left end of the line was set to "no actual feeling", and the right end was set to "have actual feeling". The tester records the degree of actual sensation as a position on a straight line (= the tester attaches a mark to a part of the line segment), and moves the distance moved to the right side (or the left side) based on the position at the start of the test as a scale value. Evaluation. Furthermore, the statistical analysis used to verify the difference between the groups was implemented by the Tukey method.

Fig. 8 is a diagram showing the results of the improvement of the degree of improvement by "question pattern", Fig. 9 regarding "wrinkles on the forehead", and Fig. 10 on "smoothness of the skin". ^* (p < 0.05) and + (p < 0.10) in the figure are those in which a significant difference or a tendency is observed with respect to the group A (placebo). In addition, in Table 2, the significant difference (p<0.05) and the tendency difference (p<0.10) which are obtained by comparison on each investigation day are represented by ○ and Δ, respectively. Based on these results, the improvement of the descriptive lines of the D group (Bilberry (RB) + emblica), the improvement of wrinkles on the forehead, and the smoothness of the skin are excellent, and as shown in Figs. 8 to 10, After 7 days, all items were confirmed to have a significant difference with respect to group A (placebo). Further, as shown in Table 2, Group D (Bilberry (RB) + Phyllanthus) is also in the C group (Bilberry (RB)) in the wrinkles of the forehead, the wrinkles on the forehead, and the smoothness (smoothness) of the skin. A significant difference (p < 0.05) or a tendency (p < 0.10) was confirmed in terms of improvement effect.

Fig. 11 shows the results of a questionnaire survey on hands and arms performed 56 days after taking it. Among all the items for evaluating skin aging, Group D (Bilberry + Phyllanthus) showed excellent improvement effects, especially in "Water Moisture", "Skin Smoothness", "Skin Gloss" In the aspect of "easy adhesion of pillow marks", "skin itching", and "transparency of skin", it was confirmed that there was a significant difference ^* (p < 0.05) or a tendency difference (p < 0.10) with respect to group A (placebo). ) The improvement effect. Thus, it was confirmed that the skin improving agent of the present invention has an excellent skin-improving effect on a part other than the face.

p‧‧‧Significant probability

R0‧‧‧Ease of ease

R1‧‧‧Ease of returning to the original position

R3‧‧‧Ease of ease

R8‧‧‧Evaluation of the effect of easy stretching and easy recovery

F3‧‧‧The area between the maximum and minimum curves of the envelope

Fig. 1 is a graph showing the results of a collagen production ability test using human skin dermal fibroblasts.

Fig. 2 is a conceptual diagram for deriving an index of viscoelasticity evaluation based on measurement data obtained by a Cutometer.

Fig. 3 is a graph showing the value of the index R0 obtained by the viscoelasticity measurement test.

Fig. 4 is a graph showing the value of the index R3 obtained by the viscoelasticity measurement test.

Fig. 5 is a graph showing the value of the index R8 obtained by the viscoelasticity measurement test.

Figure 6 is a graph showing the value of the index F3 obtained by the viscoelasticity test. Figure.

Fig. 7 is a view showing an effect of improving the wrinkles of the corners of the eyes.

Fig. 8 is a view showing the effect of improving the ruled pattern.

Fig. 9 is a view showing an effect of improving wrinkles on the forehead.

Fig. 10 is a view showing an effect of improving the smoothness of the skin.

Fig. 11 is a view showing an effect of improving the skin of a part other than the face (hand, arm).

Claims (3)

A skin improving agent for oral ingestion, which aims to improve the firmness, smoothness, wrinkles and striated lines of the skin, and is characterized by containing Vaccinium vitis-idaea and Phyllanthus emblica or Emblica officinale. Plant body or extract thereof. A collagen production promoting agent for oral ingestion, which comprises a plant body of Vaccinium vitis-idaea and Phyllanthus emblica or Emblica officinale or an extract thereof. A food or drink comprising a skin improving agent for oral ingestion according to item 1 of the patent application or a collagen production promoter for oral ingestion according to item 2 of the patent application.