JP7405543B2 - Oral skin care composition - Google Patents

Description

Article 30, Paragraph 2 of the Patent Act applies Published in "Tomato Seed Extract" (catalog published by Oryza Yuka Co., Ltd.) published on May 8, 2019

Article 30, Paragraph 2 of the Patent Act applies The 73rd Annual Conference of the Japanese Society of Nutrition and Food Science held on May 18, 2019 Shizuoka City Shimizu Cultural Hall Marinato (214 Shimazaki-cho, Shimizu-ku, Shizuoka-shi, Shizuoka Prefecture 424-0823) Shizuoka Prefectural University ( Presented at 52-1 Yata, Suruga-ku, Shizuoka City, Shizuoka Prefecture)

Application of Article 30, Paragraph 2 of the Patent Act Presented at the Japan Agricultural Chemistry Society 2019 Tokyo Conference held on March 27, 2019 at Tokyo University of Agriculture Setagaya Campus (1-1-1 Sakuragaoka, Setagaya-ku, Tokyo)

Application of Article 30, Paragraph 2 of the Patent Act Published in "Fragrance Journal, a scientific research and development journal for cosmetics and cosmetics", Volume 47, No. 1, published on January 15, 2019

The present invention relates to an oral skin care composition. The present invention is widely used in foods, medicines, skin preparations, etc.

Tomatoes (Solanum lycopersicum) are a plant that is eaten daily around the world, and are sold as juice and supplements because they contain abundant components such as vitamin C, lycopene, and GABA. Regarding the functionality of tomatoes, it has been reported that they have a blood pressure lowering effect and a cholesterol lowering effect. In addition, in 2004, new saponins, esculeosides, were identified from tomatoes (Non-patent Document 1 and Non-Patent Document 2). (Non-patent document 3). However, these findings are all related to tomato fruits, and there are few reports specific to tomato seeds.

On the other hand, collagen is a major component of the extracellular matrix in the skin, and plays an important role in maintaining skin elasticity and firmness. Elastin is a major component of the extracellular matrix other than collagen, and like collagen, elastin is an important factor in maintaining skin elasticity. It is known that collagen and elastin are produced by fibroblasts, and that their breakdown products are absorbed by the fibroblasts again. However, it is known that this cycle is disrupted and degradation products accumulate, causing changes in cell morphology and increased oxidative stress in the skin. Therefore, maintaining normal production and decomposition cycles of collagen and elastin, which constitute the extracellular matrix, is considered to be extremely important in maintaining normal skin condition.

Fujiwara S. et al., Tetrahedron, 60, 4915-4920 (2004). Ono M. et al., Chem. Pharm. Bull., 54(2), 237-239 (2006). Nohara T., J. Trad. Med., 27, 217-224 (2010). Fujifilm Co., Ltd., “Demonstrating the phenomenon that melanin pigment, which causes age spots, is decomposed within epidermal cells; confirming the effect of promoting melanin pigment decomposition in the effective whitening ingredient “AMA”, [online], November 2013 March 28, [Retrieved March 24, 2016], Internet <URL: http://www.fujifilm.co.jp/corporate/news/articleffnr_0831.html> FUJIFILM Corporation discovers that the proteolytic enzyme ``cathepsin V'' is involved in the decomposition of melanosomes, which contain the melanin pigment that causes age spots.``Oryzanol,'' contained in rice bran lipids, has been found to promote the decomposition of melanosomes. "Confirmation", [online], November 5, 2015, [Retrieved March 24, 2016], Internet <URL: http://www.fujifilm.co.jp/corporate/news/articleffnr_1020.html> Biochem. J. (1974) 137, 387-398 Matrix Biol. 2002 Jan;21(1):15-23. The Journal of Investigative Dermatology vol. 120, No. 5 may 2003. Paul Saftig, Judith KlumpermanLysosome biogenesis and lysosomal membrane proteins: trafficking meets function. Nat. Rev. Mol. Cell Biol.: 2009, 10(9);623-35 Japanese Patent Application Publication No. 2017-206468 Special table 2014-517015 publication Special Publication No. 2014-523881 Japanese Patent Application Publication No. 2015-10071

Under the above background, we conducted a clinical trial using tomato seed extract by oral intake, and as a result, we found that it has an effect of increasing dermal thickness and improving collagen density, and completed the present invention.
That is, an object of the present invention is to provide a novel composition for oral use for beautifying the skin, which has a novel effect of increasing dermal thickness and improving collagen density.

Technical features of the present invention for solving the above problems are as follows.
1. A dermis thickening agent containing tomato seed extract as an active ingredient.
2. A dermal collagen density improving agent containing tomato seed extract as an active ingredient.
3. An oral skin moisture improving agent containing tomato seed extract as an active ingredient.
4. An oral sagging skin improvement agent containing tomato seed extract as an active ingredient.
5. An oral wrinkle/fine wrinkle improving agent containing tomato seed extract as an active ingredient.
6. An oral skin firming agent containing tomato seed extract as an active ingredient.
7. Above 1. ~6. An oral skin care composition containing any one of the following agents.
8. Above 7. A food composition containing a composition of.

It is a graph showing changes in collagen score in the skin before and after ingestion of tomato seed extract. It is a graph showing changes in dermal thickness before and after ingestion of tomato seed extract. It is a graph showing changes in sensory questionnaire scores before and after ingestion of tomato seed extract.

The present invention will be explained in detail below.
The present invention is characterized in that an extract of tomato seeds is used as an active ingredient.
Tomato (scientific name: Solanum lycopersicum, English: Tomato) is a plant of the Solanaceae family, Solanaceae, that is native to the Andes highlands of South America (Peru and Ecuador). It is a perennial plant whose fruits are used as food. It is a type of green and yellow vegetable. In Japanese, it also has other names such as persimmon, red eggplant, banka, small golden melon, and coral tree eggplant.
Further, the present invention is characterized in that seeds are used as the tomato parts. This is because it contains lycoperoside, which is a saponin component unique to seeds. Examples of the lycoperoside include lycoperoside A, lycoperoside H, and the like. The lycoperoside A is a compound represented by the following chemical formula (1), and the lycoperoside H is a compound represented by the following chemical formula (2).

In addition, any method such as stirring extraction, continuous extraction, immersion extraction, or countercurrent extraction can be used to extract the active ingredients from the tomato seeds, and any method can be used at room temperature or under reflux heating. I can do it. In this case, the extraction solvent for obtaining the extract is, for example, water, lower monohydric alcohol (methyl alcohol, ethyl alcohol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohol, etc. (glycerin, propylene glycol, 1,3-butylene glycol, etc.), lower esters (ethyl acetate, etc.), hydrocarbons (benzene, hexane, pentane, etc.), ketones (acetone, methyl ethyl ketone, etc.), ethers (diethyl ether, tetrahydrofuran, etc.) , dipropyl ether, etc.), acetonitrile, etc., and one or more of them can be used. This is because lycoperoside, which is a unique component contained in tomato seeds, can be contained at a high concentration.

An example of a preferable extraction method is a method in which aqueous ethanol or aqueous methanol with a concentration of 0 to 100% (v/v) is used, extraction is performed at room temperature or with heating for 1 to 10 hours, and then filtered. Can be mentioned. This is because it is possible to obtain an extract containing active ingredients such as lycoperoside at a higher concentration.

Furthermore, a commercially available tomato seed extract may be used, and in this case, one containing the lycoperoside is preferable. Examples of these commercially available products include "Tomato Seed Extract-P" manufactured by Oryza Yuka Co., Ltd.

The oral skin beautifying composition of the present invention can be used as a material for various food and drink compositions. Examples of food and beverages include sweets (gum, candy, caramel, chocolate, cookies, snacks, jelly, gummies, tablets, etc.), noodles (soba, udon, ramen, etc.), and dairy products (milk, ice cream, yogurt, etc.). etc.), seasonings (miso, soy sauce, etc.), soups, beverages (juice, coffee, black tea, tea, carbonated drinks, sports drinks, etc.), health foods (tablets, capsules, etc.), and nutrition. Examples include supplementary foods (nutrition drinks, etc.), foods with functional claims, and foods for specified health uses. The oral beautifying composition of the present invention may be appropriately blended into these foods and drinks.

These food and drink compositions can contain various ingredients depending on their type, such as glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, and apple. Acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, Food materials such as gum arabic, carrageenan, casein, gelatin, pectin, agar, B vitamins, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances, and preservatives can be used.

The specific manufacturing method involves spray-drying or freeze-drying the oral composition for beautiful skin together with powdered cellulose, and making it into powder, granules, tablets, or solutions so that it can be easily incorporated into foods and drinks (instant foods, etc.). be able to. Further, the oral skin beautifying composition can be dissolved in, for example, oil, ethanol, glycerin, or a mixture thereof to form a liquid, and then added to a beverage or solid food. If necessary, it can be mixed with a binder such as gum arabic or dextrin to form a powder or granules, and then added to drinks or solid foods.

When the oral beautifying composition of the present invention is applied to foods and drinks, the main purpose is to maintain health and beauty, so the total content of active ingredients should be 1 to 20 wt% or less based on the food and drinks. It is preferable that

The oral composition for beautiful skin of the present invention may be used as a material for pharmaceutical compositions (including pharmaceuticals and quasi-drugs). The oral beautifying composition of the present invention can be appropriately blended with raw materials for drug preparations to produce the composition. Pharmaceutical raw materials that can be incorporated into the oral beautifying composition of the present invention include, for example, excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, kaolin, crystalline cellulose, cacao butter, hydrogenated vegetable oil, kaolin). , talc, etc.), binders (distilled water, physiological saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinylpyrrolidone, etc.), disintegrants (sodium alginate, agar, Sodium bicarbonate, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, starch, lactose, gum arabic powder, gelatin, ethanol, etc.), disintegration inhibitors (sucrose, stearin, cocoa butter, hydrogenated oil, etc.), absorption enhancers ( Examples include quaternary ammonium bases, sodium lauryl sulfate, etc.), adsorbents (glycerin, starch, lactose, kaolin, bentonite, silicic acid, etc.), and lubricants (purified talc, stearate, polyethylene glycol, etc.).

The oral composition for beautiful skin of the present invention can generally be administered orally in the form of tablets, pills, soft/hard capsules, fine granules, powders, granules, liquids, etc. However, parenteral administration may also be used. When administered as a parenteral agent, it may be administered in the form of a solution or with the addition of dispersants, suspending agents, stabilizers, etc., and may be administered locally into tissues, intradermally, subcutaneously, intramuscularly, or intravenously. can. It may also be in the form of a suppository.

Although the dosage may vary depending on the administration method, medical condition, age of the patient, etc., adults can usually receive 0.5 to 5,000 mg of the active ingredient per day, and children can usually administer about 0.5 to 3,000 mg per day.
The blending ratio of the oral skin care composition can be changed as appropriate depending on the dosage form, but it is usually about 0.3 to 15.0 wt% when administered orally or by mucosal absorption, and about 0.3 to 15.0 wt% when administered parenterally. In such cases, it is recommended to set the amount to about 0.01 to 10 wt%. Note that the dosage varies depending on various conditions, so there may be cases where a smaller amount than the above-mentioned dosage is sufficient, and there may be cases where it is necessary to administer beyond the range.

The present invention will be explained below based on examples.
Test example: Evaluation of skin beautification effect of orally ingested tomato seed extract (1) Subjects Subjects were 18 healthy men and women (average age 36.8±12.2 years). Among the subjects, 12 were female (mean age 36.7±13.6 years) and 6 were male (mean age 37.0±10.0 years).

(2) Test product and intake period (2) Test product and intake period The test product, a hard capsule containing 100 mg of “Tomato Seed Extract-P (manufactured by Oryza Yuka Co., Ltd.)” as a tomato seed extract. used. Two hard capsules were taken per day (200 mg/day intake) for 4 weeks.
The above-mentioned "Tomato Seed Extract-P" is composed of 5% tomato seed extract and 95% starch decomposition product, and contains 0.5% or more of lycoperoside in total.

(3) Evaluation method Evaluation was performed by measuring the collagen score and dermal thickness in the skin of the cheek and inner upper arm. Measurements were conducted twice: before intake (July 3, 2019) and after continued intake (July 31, 2019). Subjects were asked to remove their facial makeup 15 minutes before entering the measurement room, and were acclimatized for 15 minutes in a room with a temperature of 25.8 ± 0.9°C and humidity of 64.7 ± 3.9% before taking measurements. Collagen score and dermal thickness were measured using DermaLab® (Cortex Technology Ltd.). The above-mentioned collagen score is an index representing the density of collagen in the dermis, and the larger the value, the higher the collagen density in the dermis, and generally the dermis is rich in collagen and in good condition. We also conducted a questionnaire on skin condition before and after intake. The VAS method was used for the questionnaire.

Results and Effects of Examples (1) Collagen Score in the Skin Changes in the collagen score in the cheek and inner upper arm before and after ingestion are shown in Figure 1. As shown in Figure 1, a comparison before and after ingestion confirmed that the collagen score significantly increased in both the cheek and inner upper arm.

(2) Dermal thickness Figure 2 shows changes in dermal thickness on the cheek and inner upper arm before and after ingestion. As shown in Figure 2, a comparison before and after ingestion confirmed that the thickness of the dermis in the upper arm of the right arm had significantly increased.

(3) Experience questionnaire Changes in the experience questionnaire scores before and after intake are shown in Figure 3. As shown in Figure 3, as a result of comparing before and after intake, among the questionnaire items regarding skin sensation, "skin moisture", "improvement of sagging", "improvement of wrinkles/fine lines", and "skin firmness" were found. Significant improvements were confirmed in the following items.

(4) Effects of Example When 18 healthy adult men and women ingested Tomato Seed Extract-P at 200 mg/day for 4 weeks, the collagen score in the skin of the cheeks and inner upper arms significantly increased. It was confirmed that tomato seed extract can be used as a dermal collagen density improving agent. The thickness of the dermis also increased on the inner side of the upper arm. This confirmed that tomato seed extract can be used as a dermis thickening agent. Furthermore, in the experience questionnaire, significant improvements were confirmed in the following items: ``skin moisture,'' ``improvement of sagging,'' ``improvement of wrinkles and fine lines,'' and ``skin firmness.'' This confirmed that tomato seed extract can be used as a skin moisture improving agent, a skin sagging improving agent, a wrinkle/fine wrinkle improving agent, and a skin firmness improving agent.
Based on the above results, tomato seed extract-P increases the amount of collagen in the skin and the thickness of the dermis when taken orally, thereby contributing to the improvement of skin sagging and wrinkles. It was confirmed that the composition is useful.

Examples of formulations of the oral beautifying skin composition of the present invention are shown below, but the following formulation examples do not limit the present invention.
Formulation example 1: Chewing gum
Sugar 52.0wt%
Gum base 20.0
Glucose 10.0
Starch syrup 16.0
Fragrance 0.5
Cherry blossom extract 0.5
Glucosylceramide 0.5
Oral skin care composition 0.5
100.0wt%

Formulation example 2: Gummy
Reduced starch syrup 38.0wt%
Granulated sugar 20.0
Glucose 20.0
Gelatin 4.7
Wednesday 9.68
Kiwi juice 4.0
Kiwi flavor 0.6
Pigment 0.02
Cherry blossom extract 1.0
Glucosylceramide 1.0
Oral skin care composition 1.0
100.0wt%

Formulation example 3: Candy
Sugar 50.0wt%
Starch syrup 33.0
Water 14.2
Organic acid 2.0
Fragrance 0.2
Cherry blossom extract 0.1
Glucosylceramide 0.1
Oral skin care composition 0.4
100.0wt%

Formulation example 4: Yogurt (hard/soft)
Milk 41.5wt%
Skimmed milk powder 5.8
sugar 8.0
Agar 0.15
Gelatin 0.1
Lactic acid bacteria 0.005
Cherry blossom extract 0.1
Glucosylceramide 0.1
Oral skin care composition 0.4
fragrance trace amount
water residue
100.0wt%

Formulation example 5: Soft drink
Fructose glucose liquid sugar 30.0wt%
Emulsifier 0.5
Strawberry seed extract 0.05
Glucosylceramide 0.05
Oral skin care composition 0.05
Fragrance (appropriate amount)
Purified water remainder
100.0wt%

Formulation example 6: Soft capsule
Rice germ oil 86.0wt%
Cherry blossom extract 0.5
Glucosylceramide 0.5
Emulsifier 12.0
Oral skin care composition 1.0
100.0wt%

Formulation example 7: Tablet
Lactose 53.0wt%
Crystalline cellulose 30.0
Starch decomposition product 10.0
Cherry blossom extract 0.5
Glucosylceramide 0.5
Glycerin fatty acid ester 5.0
Oral skin care composition 1.0
100.0wt%

Formulation example 8: Granule oral preparation (pharmaceutical)
Oral skin care composition 1.0wt%
Strawberry seed extract 0.5
Glucosylceramide 0.5
Lactose 30.0
Cornstarch 60.0
Crystalline cellulose 7.0
Polyvinyl pyrrolidone 1.0
100.0wt%

Formulation example 9: Tablet confectionery
Sugar 75.4wt%
Glucose 19.0
Sucrose fatty acid ester 0.2
Strawberry seed extract 0.5
Glucosylceramide 0.5
Oral skin care composition 0.5
Purified water 3.9
100.0wt%

Formulation example 10: Cat food
Corn 33.0wt%
Flour 35.0
Meat meal 15.0
Beef tallow 8.9
Salt 1.0
Bonito extract 4.0
Strawberry seed extract 0.5
Glucosylceramide 0.5
Oral skin care composition 1.0
Taurine 0.1
Vitamins 0.5
Minerals 0.5
100.0wt%

Formulation example 11: Dog food
Corn 30.0wt%
Meat (chicken) 15.0
Defatted soybean 10.0
Flour 24.0
Rice bran 5.0
Cherry blossom extract 0.5
Glucosylceramide 0.5
Oral skin care composition 5.0
Animal fats and oils 8.9
Oligosaccharide 0.1
Vitamin 0.5
Mineral 0.5
100.0wt%

As explained above, the present invention can provide a novel oral skin care composition.

Claims (8)

An oral dermal thickening agent whose active ingredient is tomato seed extract containing lycoperoside . An oral dermal collagen density improving agent containing tomato seed extract containing lycoperoside as an active ingredient. An oral skin moisture improving agent whose active ingredient is tomato seed extract containing lycoperoside . An oral sagging skin improvement agent containing tomato seed extract containing lycoperoside as an active ingredient. An oral wrinkle/fine wrinkle improving agent whose active ingredient is tomato seed extract containing lycoperoside . An oral skin firmness improving agent whose active ingredient is tomato seed extract containing lycoperoside . An oral skin care composition containing the agent according to any one of claims 1 to 6. A food composition containing the composition of claim 7.