TW201210506A

TW201210506A - Microbicidal composition

Info

Publication number: TW201210506A
Application number: TW100145196A
Authority: TW
Inventors: Richard Levy; Megan Anne Diehl; Dolores Ann Shaw; Eileen Fleck Warwick
Original assignee: Rohm & Haas
Priority date: 2005-10-04
Filing date: 2006-09-27
Publication date: 2012-03-16
Also published as: CN101366383B; KR100873916B1; TW201210504A; CN102302016A; TW201210497A; KR20070038016A; KR100846724B1; KR20080029995A; TWI377024B; JP5192522B2; TWI377025B; TW201210491A; EP2856877A3; EP1772055A1; JP2011006453A; JP2011016815A; KR20080030002A; ES2368953T3; JP2011021018A; CN102283212A

Abstract

Synergistic microbicidal compositions containing N-(n-butyl)-1, 2-benzisothiazolin-3-one or N-methyl-1, 2-benzisothiazolin-3-one.

Description

201210506 、六、發明說明：【發明所屬之技術領域】本發明係關於經選擇抗菌劑之協同組合㈣啊⑽ combmatiori)’其具有較個別抗菌劑所觀察到更高之活性。【先前技術】於一些案例中，市售抗菌劑不能提供對微生物有效的控制，即使是於高使用濃度下，這是由於對抗某些類型的微生物（例如，彼等對某些抗菌劑具抗性者）的活性微弱，或由於侵襲性的環祕件所致。不同抗菌劑之組合有時係用以於特定目的之使用環境提供全面性對微生物的控制。例如，美國專利申請公開案第2〇〇4/〇〇14799號揭示一種 N-(正-丁基)-1，2-笨并異噻唑琳_3_酮（BBIT)與2-甲基-4-異噻唑啉-3·•酮（MI)的協同組合，其範圍限制於BBIT對MI 之比率為10 : 1至1.67 :1之内。然而，仍有需要具有抗各種品系微生物之強化活性的抗菌劑之額外組合，以提供對微生物之有效控制。再者，為了環境及經濟上的效益，係需要包含較低濃度之個別抗菌劑的組合。本發明提供該等抗菌劑之額外組合而解決此問題。【發明内容】本發明係針對一種抗菌組成物，包含：（a)N-(正-丁基)-1，2-苯并異售嗤琳-3-酮；及(b)至少一種抗菌劑，其係選自氯化苯二甲烴鈹（benzalkonium chloride)、氯化苯錢松寧（benzethonium chloride)、苯曱醇、苯并異0塞唾琳_、2-溴-2-硝基丙烷_i,3-二醇、丁二醇、辛醯二醇（capryiyi 3 93646D17 201210506 glycol)、氣笨甘醚(chlorphenesin)、1,3-二經曱基-5,5-二曱基乙内醯脲、二硫基-2,2，-雙(N-曱基苯曱醯胺）、乙二胺四乙酸或其鹽、對羥苯甲酸乙酯、己脒二羥乙基磺酸鹽 (hexamidine diisethionate)、雙辛氫咬：(hexetidine)、對經苯曱酸曱酯、苯氧基乙醇、亞麻油醯胺丙基丙二醇-氯化二曱基銨磷酸酯（linoleamidopropyl PG-dimonium chloride phosphate)、椰子油醯胺丙基丙二醇-氣化二曱基銨磷酸醋 (cocamidopropyl PG-dimonium chloride phosphate)、對經苯曱酸丙酯、山梨酸或其鹽、1-(3-氣烯丙基)-3,5,7-三氮雜-1-氮鏽金剛烷氯化物、去氫乙酸或其鹽、苯甲酸或其鹽、及檸檬酸或其鹽。本發明復針對一種抗菌組成物，包含：（a)N-甲基-l，2-苯并異噻唑啉_3_酮；及(b)至少一種抗菌劑，其係選自N-(正 -丁基)-1，2-苯并異嗔唾琳-3-酮、氯化苯二曱烴銨、氯化苯錢松寧（benzethonium chloride)、苯曱醇、苯并異嗔α坐琳酮、2-溴-2-硝基丙烷-l，3-二醇、丁二醇、辛醯二醇、氣苯甘醚、二硫基-2,2’-雙(Ν-甲基苯甲醯胺）、乙二胺四乙酸或其鹽、對羥笨甲酸乙酯、己脒二羥乙基磺酸鹽、雙辛氫啶 (hexetidine)、對羥苯甲酸曱酯、苯氧基乙醇、亞麻油醯胺丙基丙一醇-氯化二曱基按填酸醋（ljn〇leamid〇pr〇pyl PG-dimonium chloride phosphate)、椰子油醯胺丙基丙二醇 _ 氣化一曱基録填酸酉旨（cocamidopropyl PG-dimonium chloride phosphate)、山梨酸或其鹽、對羥苯曱酸丙酯、i 氯烯丙基)-3,5,7-三氮雜-1-氮鏽金剛烷氣化物、去氫乙酸或 4 93646D17 201210506 w 其鹽、苯甲酸或其鹽、檸檬酸或其鹽、及吼啶硫酮鋅（Zinc pyrithione) 〇【實施方式】 “BBIT、N_(正·丁基)-1，2_苯并異嘆唑琳-3- 嗣。”EDTA”係乙二胺四乙酸。”MBIT”係N-甲基-1，2-苯并異隹唾淋-3-酿j。於本文中使用時’除非内文另行指明’否則以下術語具有指定的定義。術語”抗菌劑，，意指一種於所在位置可殺死微生物、抑制、或控制微生物之生長之化合物；抗菌劑包含抗細菌劑、抗真菌劑及抗藻劑。術語，，微生物，，包括，例如，真菌（如酵母菌和黴菌）、細菌及藻類。術語，，所在位置（locus)”意指易遭受微生物污染之工業系統或產品。以下之縮寫於本說明書中全篇使用：ppm=每百萬分之重量份 (重量/重量）’ m]>毫升，ATCC=美國菌種中心，MBC=最小抗生物性》農度（minimum biocidal concentration)，及 MIC=最小抑制濃度。除非另行指明，否則溫度係以攝氏度數(°C)計，而百分比之關係（％)係以重量計。有機抗菌劑之量係以活性成分為基礎以ppm(重量/重量）給定。本發明之组成物意料外地發現於低於個別抗菌劑濃度的組合活性成分濃度下，具有增強的抗菌效果。於本發明之一具體實例中，彼等含齒化3-異噻唑啉酮之抗菌組成物含有其相對有低的濃度’較佳不超過.1000+ppm，更佳不超過500ppm，更佳不超過lOOppm’及最佳不超過50ppm。本發明組成物中卣化3-異噻唑啉酮之濃度係基於此組成物 5 93646D17 201210506 之活性成分之總重，亦即，不包含任何溶劑、載劑、分散劑、安定劑或其他可能存在之物質的抗菌劑。於本發明之具體實例中，該抗菌性（antimicrobial)組成物包含少於 lOOOppm，較佳不超過5〇〇ppm，更佳不超過1〇〇ppm，及最佳不超過50Ppm之5-氣_2_甲基異噻唑啉_3_酮。於本發明之一具體實例中，此抗菌性組成物包含 N-(正-丁基)-i，2-苯并異噻唑啉_3_酮及氯化苯二甲烴銨。較佳地N-(正-丁基)_ι，2_苯并異嘆„坐琳酮對氯化苯二甲煙録的重量比為自1:0.1至1:3〇，更佳為自1:〇 3至1 2〇。於本發明之一具體實例中，此抗菌性組成物包含 N-(正-丁基)-L2-苯并異噻唑啉_3_酮及氯化苯銨松寧。較佳地’ N-(正-丁基)_1>2·苯并異噻唑啉_3_酮對氯化苯銨松寧的重量比為自1:0·02至1:1.5，更佳為自1:〇.〇1至1:1。於本發明之一具體實例中，此抗菌性組成物包含 N-(正-丁基）_1，2_苯并異噻唑啉_3_酮及苯甲醇。較佳地， N-(正·丁基）_1，2_苯并異噻唑啉酮對苯曱醇的重量比為自1.1至1:1000 ’更佳為自1:2至1:8〇〇，更佳為自1:3至 1:800 〇於本發明之一具體實例中，此抗菌性組成物包含 N (正·丁基)-1，2_苯并異噻唑啉·3_軻及丨，2_苯并異噻唑啉冬綱。較佳地’队(正_丁基Μ，2-苯并異嘆哇嘛-3-酮對苯并異噻唑啉_3_酮的重量比為自1:0.02至1:20,更佳為自 1:0.02 至 1:16。於本發明之一具體實例中，此抗菌性組成物包含 6 93646D17 201210506 N-(正-丁基）-i，2-苯并異嗟哇琳_3_嗣及2溴_2_硝基丙烧 -U-二醇。較佳地’ N_(正-丁基Μ，苯并異噻唑啉冬酮對 2肩’ 2項基丙n，3_二醇的重量比為自i:〇⑴至woo。於本發明之-具體實例中，此抗菌性組成物包含 N (正-丁基）·1，2-苯并異噻唑啉_3_酮及丁二醇。較佳地， Ν (正丁基）_;ι，2_苯并異噻唑啉_3_酮對丁二醇的重量比為自I 1至1:5000 ’更佳為自1:3至1:5〇〇〇。於本發明之一具體實例中，此抗菌性組成物包含 Ν (正丁基)_ι，2_苯并異噻唑琳_3酮及辛醯二醇。較佳地， Ν (正-丁基）-ΐ，2_苯并異噻唑啉酮對辛醯二醇的重量比為自1. 1至1:1〇〇〇 ’更佳為自1:3至1:1麵。於本發明之一具體實例中，此抗菌性組成物包含 (正-丁基)-1，2-笨并異噻唑啉_3_酮及氯苯甘醚。較佳地， Ν (正-丁基)_1，2_苯并異噻唑啉_3_酮對氣苯甘醚的重量比為自1:0.5至1:700’更佳為自1:1至1:5〇〇。於本發明之一具體實例中，此抗菌性組成物包含 Ν (正-丁基)-ΐ，2_苯并異噻唑啉_3_酮及丨，3二羥曱基_5,5_ 一甲基乙内醯脲。較佳地，Ν_(正_丁基)_丨，2_苯并異噻唑啉 3-酮對1，3-二羥曱基_5,5-二曱基乙内醯脲的重量比為自1: 〇.〇2 至 1:200 ’ 更佳為自 i:〇〇6 至 ι:ΐ6〇。於本發明之一具體實例中，此抗菌性組成物包含队(正-丁基）-i，2-苯并異噻唑啉·3_酮及二硫基_2,2，_雙(N_ 甲基苯曱醯胺）。較佳地，N-(正-丁基)_ι,2-苯并異噻唑啉_3_ 綱對二硫基_2,2’-雙(’N-曱基苯甲醯胺）的重量比為自1: 93646D17 201210506 0.02 至 1:160。於本發明之一具體實例中’此抗菌性組成物包含 N-(正-丁基)-ΐ，2·苯并異噻唑啉·3酮及EDTA或其鹽，以 EDTA較佳。較佳地，N_(正_丁基苯并異噻唑啉_3•酮對EDTA或其鹽的重量比為自1:1至1:5〇〇，更佳為自1 3 至 1:500。於本發明之一具體實例中，此抗菌性組成物包含 N-(正-丁基)_ι，2_苯并異噻唑啉_3_酮及對羥苯曱酸乙酯。較佳地’ N-(正·丁基)_ι，2_苯并異噻唑啉_3_酮對對羥苯甲酸乙酯的重量比為自1:1至1:2〇〇，更佳為自1:2至1:2〇〇。於本發明之一具體實例中，此抗菌性組成物包含 N-(正-丁基）-L2·苯并異嘧唑啉_3_酮及己脒二羥乙基磺酸孤較佳地，N_(正-丁基）-1，2·苯并異噻唑啦-3-酮對己脒二羥乙基磺酸鹽的重量比為自1:〇〇2至1:3。於本發明之一具體實例中，此抗菌性組成物包含 N-(正-丁基)·ι，2·苯并異噻唑淋_3_酮及雙辛氫咬。較佳地， Ν-(正-丁基)_1，2_苯并異噻唑啉_3-嗣對雙辛氫啶的重量比為自 1:0.02 至 1:40。 ' 於本發明之一具體實例中，此抗菌性組成物包含 Ν (正丁基)_ι，2_笨并異喧唾琳_3_酮及對羥苯甲酸甲酯。較佳地Ν-(正-丁基)_ 1，2_苯并異噻唑啉_酮對對羥苯甲酸甲酯的重量比為自1:0.2至1:25〇，更佳為自1:〇5至1:24〇。於本發明之-具體實例中，此抗菌性組成物包含 Ν•(正-丁基)-1，2-苯并異噻唑啉_3_酮及·苯氧基乙醇。較佳 93646D17 8 201210506 地’N_(正-丁基)-12-苯并異噻唑啉-3-酮對苯氧基乙醇的重量比為自1:0.5至1:8〇〇，更佳為自ι:1至1:800。於本發明之一具體實例中，此抗菌性組成物包含 N'(正-丁基）_1，2_苯并異噻唑啉-3-酮及亞麻油醯胺丙基丙一醇-氣化二甲基銨碗酸酯。較佳地，N-(正-丁基)-1，2-苯并異噻唑啉-3-酮對亞麻油醯胺丙基丙二醇_氯化二甲基銨磷酸酯的重量比為自1:8至1:500。於本發明之一具體實例中，此抗菌性組成物包含 N (正-丁基）_ι，2_苯并異噻唑琳_3_酮及椰子油醯胺丙基丙一醇-氣化二甲基録碟酸酯。較佳地，N (正-丁基)_i，2_苯并異噻唑啉-3-酮對椰子油醯胺丙基丙二醇_氣化二曱基銨磷酸醋的重量比為自1:(U至1:4〇〇。於本發明之一具體實例中，此抗菌性組成物包含 N (正-丁基)_ι，2-苯并異嘆哇淋•酮及對經苯甲酸丙酯。較佳地，N-(正·丁基)-i，2_苯并異噻唑啉_3•酮對對羥苯曱酸丙西曰的重篁比為自1:1至1:5〇〇，更佳為自14至。於本發明之一具體實例中，此抗菌性組成物包含 N (正丁基)_ι，2-本并異嗟唾琳酮及山梨酸或其鹽，以山 4酸_較佳。較佳地，N_(正_丁基）4,2-苯并異嗟唑琳酮對山梨酸或其鹽的重量比為自1:6至1:12〇〇。於本發明之一具體實例中，此抗菌性組成物包含 Ν-(正-丁基）_ι，2-苯并異噻唑啉_3_酮及丨_(3氯烯丙基)-3,5,7-二氮雜氮鏽金剛烷氯化物。較佳地，Ν_(正_丁基)-1，2-苯并異嗟唾琳·3_酮對Η3·氯稀丙基)_3,5,7_三氣雜 93646D17 9 201210506 -1-氮鎬金剛烷氯化物的重量比為自1:〇 5至1:2〇〇，更佳為自 1:1 至 1:200。於本發明之一具體實例中，此抗菌性組成物包含 N-(正-丁基)-i，2-苯并異噻唑啉·3酮及去氫乙酸或其鹽，以去氫乙酸鈉較佳。較佳地，Ν_(正-丁基苯并異噻唑啉 3 _對去虱乙酸或其鹽的重量比為自1 $至I:?，更佳為自 1:0.7 至 1: 5 。於本發明之一具體實例中，此抗菌性組成物包含 Ν-(正-丁基)_1，2_笨并異噻唑啉_3•酮及苯曱酸或其鹽，以苯甲酸納較佳。較佳地，Ν_(正.丁基）]，2苯并㈣唾琳·3_ 嗣對苯甲酸或其鹽的重量比為自1:1GJL1:16GG，更佳為自 1:13 至 1:1600 〇於本發明之一具體實例中，此抗菌性組成物包含 N-(正-丁基卜丨，^苯并異噻唑啉_3_酮及檸檬酸或其鹽，以檸檬酸鈉較佳。較佳地，N_(正_丁基)_u_苯并異.售哇琳！綱對檸檬酸或其鹽的重量比為自1:5G至1:2400。於本發明之一具體實例中，此抗菌性組'成物包含N-甲基·1,2-苯并異嗟0坐琳-3-酮及N-(正·丁基)-i，2-苯并異嗟唑琳3酮較佳地，Ν_甲基i，2·苯并異嗟。坐淋綱對(正丁基）1，2本并異售°坐琳-3-酮的重量比為:自1: 〇 1至 1:30，更佳為自1:0.5至1:24。於本發明之一具體實例中，此抗菌性組成物包含Ν_ 甲基-1，2·笨并異嗟嗤琳_3_酮及氯化苯二甲烴銨。較佳地， Ν-甲基-1，2-笨并異噻唑啉_3_酮對氯化苯二甲烴銨的重量 93646D17 10 201210506 比為自1: Ο.1至1:2〇，更佳為自1:0.2至1:20。於本卷明之一具體實例中，此抗菌性組成物包含] 曱基-1，2-笨并異噻唑啉_3_酮及氯化苯銨松寧。較佳地，N_ 甲基-1,2-苯并異噻唑啉_3酮對氯化苯銨松寧的重量比為自1: 0.3至1:0.6 ’更佳為自1:〇 4至1:〇 6。於本發明之一具體實例中，此抗菌性組成物包含曱基-1，2-苯并異噻唑啉_3_酮及苯甲醇。較佳地，n_甲基 -1，2-苯并異嗟唾啉_3_酮對苯甲醇的重量比為自1:丨至 1:800 ’更佳為自1:5至1:8〇〇。於本發明之一.具體實例中’此抗菌性組成物包含Ν· 甲基-1，2-苯并異噻唑啉酮及苯并異噻唑啉酮。較佳地， N-曱基-1，2-苯并異噻唑啉_3_酮對苯并異噻唑啉酮的重量比為自1:(U至，更佳為自1:0.3至1:20。於本發明之一具體實例中，此抗菌性組成物包含N-甲基_1，2_苯并異嘆0坐琳-3-酉同及2-漠-2-硝基丙-1，3·二醇。較佳地’ Ν-甲基-1，2-苯并異噻唑啉-3-酮對2-溴-2-硝基丙烷 _1，3_二醇的重量比為自1:0.1至1:100。於本發明之一具體實例中，此抗菌性組成物包含Ν- 曱基-1，2-苯并異噻唑啉酮及丁二醇。較佳地，甲基 -1，2-苯并異嗔唾啉_3_酮對丁二醇的重量比為自I」至 1:400，更佳為自1:13至1375。於本發明之一具體實例中，此抗菌性組成物包含Ν-甲基·1’2·苯并異嘆„坐琳_3_酮及辛醯二醇。較佳地，甲基 -1，2-苯并異嗟哇淋_3_酮對辛醯二醇的重量比為自ία至 11 93646D17 201210506 1:1500。於本發明之一具體實例中，此抗菌性組成物包含N_ 曱基-1，2-苯并異噻唑啉_3_酮及氯苯甘醚。較佳地，甲基 _1，2_苯并異噻唑啉-3-酮對氯苯甘醚的重量比為自1:1至 1:250 ’更佳為自1:5至1:250。於本發明之一具體實例中’此抗菌性組成物包含N-曱基-1，2-苯并異噻唑啉_3_酮及二硫基_2,2，_雙(n-甲基苯甲醯胺）。較佳地，N-曱基-1，2-苯并異噻唑啉-3-酮對二硫基 -2,2 -雙(N-甲基苯甲醯胺）的重量比為自1:〇^至i 1〇〇。於本發明之一具體實例中，此抗菌性組成物包含N· 甲基-1，2_笨并異噻唑啉-3-酮及EDTA或其鹽，以EDTA較佳。較佳地，甲基_i，2-苯并異噻唑啉-3-酮對EDTA或其鹽的重量比為自1: 1至1:400，更佳為自1:1〇至1:320。於本發明之一具體實例中，此抗菌性組成物包含N-甲基-1,2-笨并異噻唑啉_3_酮及對羥苯甲酸乙酯。較佳地， N-甲基·1，2-苯并異噻唑啉·3酮對對羥苯甲酸乙酯的重量比為自1.5至1:5〇〇，更佳為自I:?至ι:4〇〇。於本發明之一具體實例中，此抗菌性組成物包含Ν-甲基-1，2-苯并異噻唑啉_3•酮及己脒二羥乙基磺酸鹽。較佳地’ NHi，2_苯并異嘆0坐琳_3•晒對己脉三經乙基續酸鹽的重量比為自1:〇m:5,更佳為自1:〇1至1:3。於本，明之一具體實例中，此抗菌性組成物包含N-甲基-j，2-苯并異噻唑啉_3_酮及雙辛氫啶。較佳地，甲基丄2-苯并異嗟哇琳·3·酮對雙辛氫咬的重量比為自1:0.1至 93646D17 12 201210506 ' 1:40。於本發明之一具體實例中，此抗菌性組成物包含N-甲基-1，2-苯并異噻唑啉_3_酮及對羥苯甲酸甲酯。較佳地， N-曱基_1，2-苯并異噻唑啉_3_酮對對羥苯甲酸曱酯的重量比為自1:1至1:400，更佳為自1:2至1:4〇〇。於本發明之一具體實例中，此抗菌性組成物包含N_ 甲基-1，2-苯并異噻唑啉_3_酮及苯氧基乙醇。較佳地，N_ 曱基-1，2-苯并異噻唑啉_3_酮對苯氧基乙醇的重量比為自 1:120 至 1:16〇。於本發明之一具體實例中，此抗菌性組成物包含N_ 曱基-1，2-苯并異噻唑啉_3_酮及亞麻油醯胺丙基丙二醇-氣化二曱基銨磷酸酯。較佳地，N_甲基_i，2_苯并異噻唑啉_3_ 酮對亞麻油酿胺丙基丙二醇_氣化二甲基銨罐酸醋的重量比為自1:3至1:400。於本發明之一具體實例中，此抗菌性組成物包含N_ 甲基-1，2-苯并異噻唑啉酮及椰子油醯胺丙基丙二醇-氣化二曱基銨磷酸酯。較佳地，N_曱基_丨，2_苯并異噻唑啉_3_ 酮對椰子油醯胺丙基丙二醇_氣化二甲基銨磷酸酯的重量比為自1:0.1至1:125，最佳為自k〇.4至1:125。於本發明之一具體實例中，此抗菌性組成物包含N_ 曱基-1，2-苯并異噻唑啉_3_酮及山梨酸或其鹽，以山梨酸鉀較佳。較佳地’ N-甲基-1，2·苯并異噻唑琳_3_酮對山梨酸或其鹽的重量比為自1:5至1:6〇〇，更佳為自1:8至1:6〇〇。於本發明之一具體實例中，此抗菌性組成物包含Ν_ 93646D17 13 201210506 甲基_1，2_苯并異噻唑啉_3_酮及對羥苯甲酸丙酯。較佳地， N-甲基·1，2-苯并異噻唑琳_3_酮對對羥苯甲酸丙酯的重量比為自1:10至1:1200 ’更佳為自1:13至1:12〇〇。於本發明之一具體實例申，此抗菌性組成物包含Ν- 甲基-1，2-苯并異噻唑啉_3_酮及順小(3氯烯丙基)·3,5,7三氮雜-1-氮鏽金剛烷氯化物。較佳地，Ν_甲基“，、苯并異噻坐琳3嗣對順-1_(3_氣晞丙基)·3,5,7_三氮雜·ι_敗鏽金剛烧氯化物的重量比為自1:0.2至1:400,更佳為自1:0.3至 1:200。於本發明之一具體實例中，此抗菌性組成物包含Ν· 甲基-1，2-苯并異噻唑啉_3_酮及去氫乙酸或其鹽，以去氫乙酸鈉較佳。較佳地，Ν_甲基苯并異噻唑啉_3_酮對去氫乙酸或其鹽的重量比為自1:1至1:5。於本發明之一具體實例中，此抗菌性組成物包含Ν-甲基-1，2-苯并異噻唑啉_3_酮及苯甲酸或其鹽，以苯曱酸鈉較佳較佳地’ Ν-甲基-ΐ，2-苯并異嗟唾淋酮對苯曱酸或其鹽的重量比為自I]至1:_，更佳為自1:4至1:_。於本發明之一具體實例中，此抗菌性組成物包含Ν-甲基-1，2·苯并異噻唑啉_3_酮及檸檬酸或其鹽，以檸檬酸鈉較佳較地，Ν-曱基·ι，2_苯并異養唾琳_3·嗣對擦樣酸或其鹽的重量比為自1:30至1:2500,更佳為自1:33至1:24〇〇。於本發明之一具體實例中，此抗菌性組成物包含Ν-甲基1,2本并異嘆唾琳_3_酮及η比咬硫_鋅。較佳地，Ν_ 甲基-1，2-苯并異噻唑啉_3_酮對吡啶硫酮鋅的重量比為自 93646D17 14 201210506 1:0.01 至 1:6,更佳為自 1:〇〇4至1:4。本發明組成物中的抗菌劑可“以其本 =固=配而使用。適合的溶劑包括：先水’一酵如乙一醇、丙二醇、二乙二醇乙二醇及聚丙二醇；二醇一一醇、聚叫，私，如甲醇、乙醇、而醢、苯乙醇及苯氧基丙醇…如丙綱及甲基乙基綱；如乙酸乙醋、乙酸丁輯、禪檬酸三乙醋及三乙酸甘油醋？酸醋，如碳酸伸丙酿及碳酸二甲醋；及上述各溶劑之混：物。較佳地，該溶劑係選自水、二醇、二軸、二各種溶劑之混合物。適合之固態_包括，例如二氧㈣、料土、躐、纖維素材料、驗金族及驗土族(例如，納、鎮、鉀)金屬鹽（例如，氯化物、石肖酸鹽、淳化物、硫酸鹽)及炭。當抗菌成分調配於溶劑中，此配方可視需要包含界面活性劑。當此配方包含界面活性劑，其通常呈乳化濃縮劑 (emulsive concentrates)、乳化液、微乳化濃縮劑 (microemulsive concentrates)、或微乳化液。乳化濃縮劑在添加足量的水之後立即形成乳化液。微乳化濃縮劑在添加足量的水之後立即形成微乳化液。此等乳化濃縮劑或微乳化濃縮劑為此技藝中所普遍習知；此等配方較佳不含界面活性劑。美國冬利案第5,444,078號可查閱進一步製備各種微乳化液及微乳化濃縮劑之一般及特定細節。抗菌成分亦可調配成分散液之形式。分散液之溶劑成分可為有機溶劑或水，以水較佳。此等分散液可包含佐劑， 15 93646D17 201210506 例如.共'容劑（c〇-solvent)、增稠劑、抗凍劑、分散劑、填充齊丨色素、界面活性劑、生物分散劑、續酸基琥珀酸鹽、 ίΙ稀、°夫°南_、多價陽離子、安定劑、阻垢劑及防腐敍添加劑。當兩種抗菌劑均各自先與溶劑調配時，第一種抗菌劑所用之溶劑可與用以調配另一種商業可得之抗菌劑之溶劑相同或相異’然而對多數工業抗生物劑之應用，水係較佳。較佳係兩種溶劑為可混溶的。爛熟於此技藝之人應瞭解本發明之抗菌成分可先後、同時加至所在位置，或於加至該所在位置前合併。較佳係第一種抗菌劑與第二種抗菌成分同時或先後加至所在位置。當該等抗菌劑係同時或先後添加，個別成分可能各可含佐劑’像是，例如：溶劑、增稠劑、抗束劑、著色劑、鉗合劑（如乙二胺四乙酸、乙二胺二琥珀酸、亞胺二琥珀酸及其鹽）、分散劑、界面活性劑、生物分散劑、續酸基琥珀酸鹽、萜烯、呋喃酮、多價陽離子、安定劑、阻垢劑及防腐#添加劑。本發明之抗菌組成物可藉由於易遭受微生物攻擊之所在位置的上方、内部、或於其所在之處引入抗菌有效量的組成物’而用以抑制微生物或較高等水生生物（如原蟲、無脊椎動物、苔蘚蟲、雙鞭毛藻、曱殼類、軟體動物等）之生長。適合的所在位置包括，例如：工業用水；電塗沉積系統(electrocoat deposition system);冷卻塔；空氣清洗器；氣體洗滌器；礦漿；廢水處理；裝飾喷泉；逆滲透過 16 93646D17 201210506 濾；超過濾(ultrafiltration);壓載水；蒸發式冷凝器；熱交換器；製漿造紙溶液及添加劑；澱粉；塑膠；乳化液；分散劑；顏料；乳汁；塗料，如清漆；建築製品，如膠黏水泥、嵌縫膏及密封膠’建桌黏合劑’如陶竟黏合劑、地毯底布黏合劑及複合黏合劑；工業用或消費性黏合劑；攝影化學品；印刷液體；家居用品，如浴室及磨房清潔用品；美容用品；化妝品；洗髮精；肥皂；清潔劑；工業用清潔劑’地板光<72劑，洗衣房洗條水，金屬加工液體；輸送帶潤滑液；液壓液體(hydraulic fluid);皮革及皮革製品；纺織物’纺織產品；木材及木製品’如膠合板、碎木片板 (chipboard)、牆板、削片板(fiakeboard)、層合樑、定向粒片板、硬板及粒片板；石油煉製液體；燃料；油田液體，如注射水、裂隙液體及鑽井泥；農業佐劑保存\界面活性劑保存；醫療儀器；診斷試劑保存；食物保存，如塑膠或紙類食物包膜；食物、飲料及工業程序用之巴氏滅菌器 (pasteurizer);馬桶；遊憩用水；池塘；及礦泉。較佳地，本發明之抗菌組成物係用以於選自下列一種或多種之所在位置抑制微生物的生長：礦裝；製裝造紙溶液及添加劑；澱粉；乳化液；分散劑；顏料；乳汁；塗料；建築黏合劑，如陶絲合劑、地毯底布黏合劑；攝影化學品；印刷液體；家居用品，如浴室及厨房清潔用品；美容 1:二匕:品;洗髮精;肥息；清潔劑；工業用清潔劑；農二洗衣房洗蘇水;金屬加工液體；紡織產品；劑保存；界面活性劑保存；診斷試劑保存；食物保 93646D17 17 201210506 存；食物、飲料及工業程序用之巴氏滅菌器。於所在位置抑制或控制微生物及較高等的水生生物生長所需之本發明組成物的明確量係取決於欲保護之特定所在位置。典型地，若本發明組成物於所在位置提供該組成物中0.1至1 OOOppm異嗟唾淋成分，則該組成物的量係足以於該所在位置控制微生物的生長。該組成物之異噻唑琳成为存在於此所在位置的量以至少〇 5ppm較佳，至少 4ppm更佳及至少i〇ppm最佳。該組成物之異噻唑啉成分存在於此所在位置的量以不超過1000ρριη較佳，不超過 500ppm更佳及不超過2〇〇ppm最佳。於本發明之一具體實例中，此組成物為實質上不含酵素性抗生物劑。較佳地，當BBIT或MBIT與對經苯甲酸曱酯或對羥苯曱酸乙酯其中之一組合時，此組成物為實質上不含酵素性抗生物劑。酵素性抗生物劑為具有抗微生物活性之酵素，其係定義，例如，於美國專利申請公開案第 2002/0028754 號。 (實施例）材料與方法本發明組合的協同作用係藉檢驗廣範圍的化合物濃度及比例而予以驗證。一種協同作用的測量係Kull，F.C. ; Eisman，P.C.; Sylwestrowicz, H.D.及 Mayer, R.L.描述於 Applied Microbiology 9 : 538-541(1961)之工業上接受的方法，其係 93646D17 201210506 使用依下式所決定的比例：201210506, VI. DESCRIPTION OF THE INVENTION: TECHNICAL FIELD OF THE INVENTION The present invention relates to a synergistic combination of selected antimicrobial agents (4) (10) combmatiori) which has a higher activity than that observed with individual antimicrobial agents. [Prior Art] In some cases, commercially available antimicrobials do not provide effective control of microorganisms, even at high use concentrations, due to resistance to certain types of microorganisms (eg, they are resistant to certain antimicrobial agents). The sexual activity is weak, or due to invasive ring secrets. Combinations of different antimicrobial agents are sometimes used to provide comprehensive microbial control for a specific purpose use environment. For example, U.S. Patent Application Publication No. 2/4/14,799 discloses an N-(n-butyl)-1,2-benzisothiazolidine-3-butanone (BBIT) and 2-methyl- A synergistic combination of 4-isothiazoline-3··one (MI), the range of which is limited to a BBIT to MI ratio of 10:1 to 1.67:1. However, there is still a need for additional combinations of antimicrobial agents that have enhanced activity against various strains of microorganisms to provide effective control of microorganisms. Furthermore, for environmental and economic benefits, it is desirable to include a combination of lower concentrations of individual antimicrobial agents. The present invention provides an additional combination of such antimicrobial agents to solve this problem. SUMMARY OF THE INVENTION The present invention is directed to an antibacterial composition comprising: (a) N-(n-butyl)-1,2-benzoisoindolizin-3-one; and (b) at least one antibacterial agent , which is selected from the group consisting of benzalkonium chloride, benzethonium chloride, benzoquinone, benzopyrene, and 2-bromo-2-nitropropane _i,3-diol, butanediol, octanediol (capryiyi 3 93646D17 201210506 glycol), chlorphenesin, 1,3-dipyridyl-5,5-didecyl Urea urea, dithio-2,2,-bis(N-mercaptobenzamide), ethylenediaminetetraacetic acid or its salt, ethyl p-hydroxybenzoate, hexamethylene dihydroxyethyl sulfonate ( Hexamidine diisethionate), hexetidine, phthalic acid phenoxy phthalate, phenoxyethanol, linoleamidopropyl PG-dimonium chloride phosphate Cocamidopropyl PG-dimonium chloride phosphate, propyl benzoate, sorbic acid or its salt, 1-(3-allyl allyl) -3,5,7 - Triaza-1-nitrogenadamantane chloride, dehydroacetic acid or a salt thereof, benzoic acid or a salt thereof, and citric acid or a salt thereof. The present invention is directed to an antibacterial composition comprising: (a) N-methyl-l,2-benzisothiazolin-3-enone; and (b) at least one antibacterial agent selected from N-(positive) -butyl)-1,2-benzisoindole-3-one, benzodiazepine chloride, benzethonium chloride, benzoquinone, benzopyrene Ketone, 2-bromo-2-nitropropane-l,3-diol, butanediol, octanediol, gas phenylglycolate, dithio-2,2'-bis(indole-methylbenzene Indoleamine, ethylenediaminetetraacetic acid or its salt, ethyl p-hydroxybenzoate, hexamethylene dihydroxyethyl sulfonate, hexetidine, decyl paraben, phenoxyethanol, Linseed oil, guanidinium propyl propyl alcohol - chlorinated diacetate (ljn〇leamid 〇pr〇pyl PG-dimonium chloride phosphate), coconut oil allylpropyl propylene glycol _ gasification Cocamidopropyl PG-dimonium chloride phosphate, sorbic acid or its salt, propyl paraben, i-chloroallyl)-3,5,7-triaza-1-nitrogen adamantane vapor , dehydroacetic acid or 4 93646D17 201210506 w its salt, benzoic acid or its salt, lemon Or a salt thereof, and Zinc pyrithione 〇 [embodiment] "BBIT, N_(n-butyl)-1,2_benzoisoxazole-3-ene." EDTA" is B Diaminetetraacetic acid. "MBIT" is N-methyl-1,2-benzisoindole-3-brown j. As used herein, the following terms have the specified definitions unless the context dictates otherwise. The term "antibacterial agent" means a compound that kills, inhibits, or controls the growth of microorganisms at a location; the antimicrobial agent comprises an antibacterial, antifungal, and antialgatic agent. The term "microorganisms" includes, for example, fungi (such as yeasts and molds), bacteria and algae. The term "locus" means an industrial system or product susceptible to microbial contamination. The following abbreviations are used throughout this specification: ppm = parts per million by weight (weight/weight) 'm]&gt ; milliliters, ATCC = US strain center, MBC = minimum bioavailability, minimum MIC = minimum inhibitory concentration. Unless otherwise indicated, temperatures are in degrees Celsius (°C), and percentages The relationship (%) is by weight. The amount of the organic antibacterial agent is given in ppm (weight/weight) based on the active ingredient. The composition of the present invention is unexpectedly found in concentrations of the combined active ingredient below the concentration of the individual antimicrobial agent. The antibacterial composition has an enhanced antibacterial effect. In one embodiment of the present invention, the antibacterial composition containing the toothed 3-isothiazolinone has a relatively low concentration of preferably not more than .1000+ppm. Preferably, it is not more than 500 ppm, more preferably not more than 100 ppm' and most preferably not more than 50 ppm. The concentration of deuterated 3-isothiazolone in the composition of the present invention is based on the total weight of the active ingredient of the composition 5 93646D17 201210506 That is, an antibacterial agent that does not contain any solvent, carrier, dispersant, stabilizer, or other substance that may be present. In a specific embodiment of the invention, the antimicrobial composition comprises less than 1000 ppm, preferably No more than 5 〇〇 ppm, more preferably no more than 1 〇〇 ppm, and preferably no more than 50 pm of 5-a gas 2-methylisothiazoline _3 ketone. In one embodiment of the invention, the antimicrobial The composition comprises N-(n-butyl)-i,2-benzisothiazolin-3-enone and chlorodimethylammonium chloride. Preferably N-(n-butyl)_ι, 2_ The weight ratio of benzophenone to chlorinated benzophenone is from 1:0.1 to 1:3 〇, more preferably from 1:3 to 12 〇. In one embodiment of the invention, the antimicrobial composition comprises N-(n-butyl)-L2-benzisothiazoline-3-one and benzylammonium chloride. Preferably, the weight ratio of 'N-(n-butyl)_1>2·benzisothiazolin-3-enone to benzylammonium chloride is from 1:0.02 to 1:1.5, more preferably From 1: 〇.〇1 to 1:1. In one embodiment of the invention, the antimicrobial composition comprises N-(n-butyl)-1,2-benzisothiazoline-3-one and benzyl alcohol. Preferably, the weight ratio of N-(n-butyl)_1,2-benzisothiazolinone to benzofuran is from 1.1 to 1:1000', preferably from 1:2 to 1:8〇〇 More preferably, it is from 1:3 to 1:800. In one embodiment of the present invention, the antibacterial composition comprises N (n-butyl)-1,2-benzisothiazoline·3_轲 and丨, 2_benzisothiazoline winter class. Preferably, the weight ratio of 'team (n-butyl hydrazine, 2-benzo isopropyl keto-3-one to benzoisothiazoline _3 ketone) is from 1:0.02 to 1:20, more preferably From 1:0.02 to 1:16. In one embodiment of the invention, the antimicrobial composition comprises 6 93646D17 201210506 N-(n-butyl)-i,2-benziso Wahlin_3_嗣And 2 bromo-2-nitropropane-U-diol. Preferably 'N_(n-butyl fluorene, benzisothiazolinone) to 2 shoulders ' 2 yl, n, 3 diol The weight ratio is from i: 〇 (1) to woo. In a specific embodiment of the invention, the antimicrobial composition comprises N (n-butyl)·1,2-benzisothiazoline _3-ketone and butyl Preferably, the weight ratio of Ν(n-butyl)_;ι,2_benzisothiazoline_3_one to butanediol is from I 1 to 1:5000 'better than 1:3 To 1:5. In one embodiment of the invention, the antimicrobial composition comprises Ν(n-butyl)_ι, 2-benzisothiazolidine-3-one and octanediol. Preferably, , Ν (n-butyl)-fluorene, the weight ratio of 2_benzisothiazolinone to octanediol is from 1.1 to 1:1 更' more preferably from 1:3 to 1: 1 side. One of the inventions In the embodiment, the antibacterial composition comprises (n-butyl)-1,2-benzisothiazoline-3-one and chlorophenylglycol. Preferably, Ν(n-butyl)_1,2 The weight ratio of benzoisothiazoline _3 ketone to gas phenyl glycol is from 1:0.5 to 1:700', more preferably from 1:1 to 1:5 〇〇. In one embodiment of the present invention The antibacterial composition comprises Ν(n-butyl)-oxime, 2_benzisothiazolin-3-enone and hydrazine, 3 bishydroxyindole _5,5-monomethyl carbendazol. The weight ratio of Ν_(正-butyl)_丨, 2_benzisothiazolin-3-one to 1,3-dihydroxydecyl_5,5-dimercaptoindolide is from 1: 〇.〇2 to 1:200' More preferably from i: 〇〇6 to ι: ΐ6〇. In one embodiment of the invention, the antimicrobial composition comprises a team (n-butyl)-i, 2 - Benzoisothiazoline·3-ketone and disulfo-2,2,-bis(N-methylbenzoguanamine). Preferably, N-(n-butyl)_ι,2-benziso The weight ratio of thiazoline-3_2 to dithio-2,2'-bis('N-mercaptobenzamide) is from 1:93646D17 201210506 0.02 to 1:160. In one embodiment of the present invention 'This antibacterial composition contains N-(positive - Butyl)-oxime, 2·benzisothiazolin-3-one and EDTA or a salt thereof, preferably EDTA. Preferably, N_(n-butylbenzisothiazoline-3)one is used for EDTA or The weight ratio of the salt is from 1:1 to 1:5 Torr, more preferably from 13 to 1:500. In one embodiment of the invention, the antimicrobial composition comprises N-(n-butyl)-, 2-benzisothiazoline-3-one and ethyl p-hydroxybenzoate. Preferably, the weight ratio of 'N-(n-butyl)_ι, 2_benzisothiazoline-3-one to ethyl p-hydroxybenzoate is from 1:1 to 1:2 Torr, more preferably From 1:2 to 1:2. In one embodiment of the present invention, the antimicrobial composition comprises N-(n-butyl)-L2.benzoisoxazoline-3-enone and hexamethylene dihydroxyethylsulfonate. Preferably, The weight ratio of N_(n-butyl)-1,2·benzisothiazol-3-one to hexamethylene dihydroxyethyl sulfonate is from 1:2 to 1:3. In one embodiment of the invention, the antimicrobial composition comprises N-(n-butyl)·ι, 2· benzisothiazolidine-3-one and dioctane hydrogen bit. Preferably, the weight ratio of Ν-(n-butyl)_1,2-benzisothiazoline_3-oxime to bisoctylhydropyridine is from 1:0.02 to 1:40. In one embodiment of the invention, the antimicrobial composition comprises Ν(n-butyl)_ι, 2_ 并并喧琳 _ _ _ _ ketone and methylparaben. Preferably, the weight ratio of Ν-(n-butyl)-1 1,2-benzisothiazolinone to methylparaben is from 1:0.2 to 1:25 Torr, more preferably from 1: 〇5 to 1:24〇. In a specific embodiment of the invention, the antimicrobial composition comprises Ν•(n-butyl)-1,2-benzisothiazolin-3-enone and phenoxyethanol. Preferably, 93646D17 8 201210506 The weight ratio of 'N_(n-butyl)-12-benzisothiazolin-3-one to phenoxyethanol is from 1:0.5 to 1:8 Torr, more preferably from ι:1 to 1:800. In one embodiment of the present invention, the antimicrobial composition comprises N'(n-butyl)-1,2-benzisothiazolin-3-one and linseed guanidiniumpropylpropanol-gasification Methyl ammonium phthalate. Preferably, the weight ratio of N-(n-butyl)-1,2-benzisothiazolin-3-one to linoleum allylpropyl propylene glycol dimethylammonium chloride phosphate is from 1: 8 to 1:500. In one embodiment of the present invention, the antimicrobial composition comprises N (n-butyl)_ι, 2-benzisothiazolidine-3-ol and coconut oil guanamine propylpropanol-gasification Base recording disc ester. Preferably, the weight ratio of N(n-butyl)_i,2-benzisothiazolin-3-one to coconut oil decyl propyl propylene glycol _ gasified decyl ammonium phosphate vinegar is from 1: (U To 1:4. In one embodiment of the present invention, the antibacterial composition comprises N (n-butyl)_ι, 2-benzoisosyl ketone and propyl benzoate. Preferably, the ratio of N-(n-butyl)-i,2_benzisothiazoline_3·ketone to propionate of p-hydroxybenzoic acid is from 1:1 to 1:5〇〇, More preferably, it is from 14 to 1. In one embodiment of the present invention, the antibacterial composition comprises N (n-butyl)_ι, 2-benzisoindolone and sorbic acid or a salt thereof, Preferably, the weight ratio of N_(n-butyl) 4,2-benzisoxazolinone to sorbic acid or a salt thereof is from 1:6 to 1:12 Å. In one embodiment, the antimicrobial composition comprises Ν-(n-butyl)_ι, 2-benzisothiazoline-3-enone and 丨_(3 chloroallyl)-3,5,7- Nitroza rust adamantane chloride. Preferably, Ν_(n-butyl)-1,2-benzisoindolyl-3-ketone to Η3·chloropropyl)_3,5,7_三气杂93646D17 9 2012105 The weight ratio of 06-1-n-adamantine chloride is from 1:5 to 1:2, more preferably from 1:1 to 1:200. In one embodiment of the present invention, the antibacterial composition comprises N-(n-butyl)-i,2-benzisothiazolin-3-one and dehydroacetic acid or a salt thereof, and sodium dehydroacetate is compared. good. Preferably, the weight ratio of Ν_(n-butylbenzisothiazoline 3 _ to deacetic acid or a salt thereof is from 1 $ to I:?, more preferably from 1:0.7 to 1:5. In one embodiment of the invention, the antibacterial composition comprises Ν-(n-butyl)_1,2-p-isoisothiazoline-3 ketone and benzoic acid or a salt thereof, preferably sodium benzoate. Preferably, Ν_(n-butyl)], 2 benzo(tetra) salin·3_ 嗣 benzoic acid or its salt is from 1:1GJL1:16GG, more preferably from 1:13 to 1:1600 〇 In a specific embodiment of the present invention, the antibacterial composition comprises N-(n-butyl hydrazine, benzoisothiazoline -3- ketone, and citric acid or a salt thereof, preferably sodium citrate. Preferably, N_(n-butyl)_u_benzal is different. The weight ratio of citric acid or its salt is from 1:5G to 1:2400. In one embodiment of the present invention, this The antibacterial group's composition contains N-methyl·1,2-benzisoindole 0 sitin-3-one and N-(n-butyl)-i,2-benzisoxazole 3 ketone Jiadi, Ν_Methyl i,2·Benzene isoindole. The weight ratio of S. sylvestris (n-butyl) 1, 2 and the sale of Selen-3-one is from 1: 〇1 to 1:30, More preferably, it is from 1:0.5 to 1:24. In one embodiment of the present invention, the antibacterial composition comprises Ν_methyl-1,2· stupid isoindene _3-ketone and chlorinated benzene Ammonium methylammonium. Preferably, the weight of Ν-methyl-1,2-p-isoisothiazoline-3-one-p-chlorobenzylammonium chloride is 93646D17 10 201210506 ratio is from 1: Ο.1 to 1: 2〇, more preferably from 1:0.2 to 1:20. In one embodiment of the present disclosure, the antimicrobial composition comprises] mercapto-1,2-p-isoisothiazolin-3-enone and chlorination Preferably, the weight ratio of N-methyl-1,2-benzisothiazolin-3-one to benzylammonium chloride is from 1:0.3 to 1:0.6' more preferably from 1 : 〇 4 to 1: 〇 6. In one embodiment of the invention, the antimicrobial composition comprises decyl-1,2-benzisothiazolin-3-one and benzyl alcohol. Preferably, n_ The weight ratio of methyl-1,2-benzisoindole porphyrin _3-ketone to benzyl alcohol is from 1: 丨 to 1:800 ′ more preferably from 1:5 to 1:8 〇〇. In a specific example, 'this antibacterial composition comprises Ν·methyl-1,2-benzisothiazolinone and benzisothiazolinone. Preferably, N-mercapto-1,2-benzene The weight ratio of the isothiazoline -3- ketone to the benzisothiazolinone is from 1: (U to, more preferably from 1: 0.3 to 1: 20). In one embodiment of the invention, the antimicrobial composition The product comprises N-methyl-1,2_benzoxanthene 0 sitting -3- mer and 2-moly-2-nitropropane-1,3·diol. Preferably ''-methyl- The weight ratio of 1,2-benzisothiazolin-3-one to 2-bromo-2-nitropropane-1,3-diol is from 1:0.1 to 1:100. In one embodiment of the invention, the antimicrobial composition comprises indole-indenyl-1,2-benzisothiazolinone and butylene glycol. Preferably, the weight ratio of methyl-1,2-benzisoindole porphyrin-3-one to butanediol is from I" to 1:400, more preferably from 1:13 to 1375. In a specific embodiment of the present invention, the antibacterial composition comprises Ν-methyl·1'2·benzo-iso-supplementary _3-ketone and octanediol. Preferably, methyl-1, The weight ratio of 2-benzisoindole _3 ketone to octyl diol is from ία to 11 93646D17 201210506 1:1500. In one embodiment of the invention, the antimicrobial composition comprises N 曱 - - 1,2-Benzisothiazolin-3-yl ketone and chlorophenyl glycol ether. Preferably, the weight ratio of methyl-1,2-benzisothiazolin-3-one p-chlorobenzoate is from 1 More preferably from 1:5 to 1:250. In one embodiment of the invention, the antimicrobial composition comprises N-mercapto-1,2-benzisothiazoline_3 -ketone and disulfo-2,2,_bis(n-methylbenzamide). Preferably, N-mercapto-1,2-benzisothiazolin-3-one p-dithio The weight ratio of -2,2-bis(N-methylbenzamide) is from 1: 〇^ to i 1 〇〇. In one embodiment of the invention, the antimicrobial composition comprises N·methyl -1,2- benzoisothiazolin-3-one and EDTA or a salt thereof, preferably EDTA. Preferably, methyl-i,2-benzisothiazolin-3-one to EDTA or a salt thereof Heavy The ratio is from 1:1 to 1:400, more preferably from 1:1 to 1:320. In one embodiment of the invention, the antimicrobial composition comprises N-methyl-1,2-stupid Isothiazoline-3-ol and ethyl p-hydroxybenzoate. Preferably, the weight ratio of N-methyl·1,2-benzisothiazolin-3-one to ethyl p-hydroxybenzoate is from 1.5 to 1:5〇〇, more preferably from I:? to ι:4〇〇. In one embodiment of the invention, the antimicrobial composition comprises Ν-methyl-1,2-benzisothiazoline_ 3 • ketone and hexamethylene dihydroxyethyl sulfonate. Preferably, 'NHi, 2_ benzopyrene 0 sitting _3 • sun to the pulse of three ethyl phthalate weight ratio from 1: 〇m: 5, more preferably from 1: 〇1 to 1:3. In one embodiment of this, the antibacterial composition comprises N-methyl-j,2-benzisothiazoline_3_ Ketone and dioctylhydrogenidine. Preferably, the weight ratio of methyl hydrazine 2-benzhydrazinone 3 ketone to dioctane hydrogen bitrate is from 1:0.1 to 93646D17 12 201210506 ' 1:40. In one embodiment of the invention, the antimicrobial composition comprises N-methyl-1,2-benzisothiazolin-3-enone and methylparaben. Preferably, N-mercapto-1, The weight ratio of 2-benzisothiazoline-3-enone to decyl paraben is from 1:1 to 1:400, more preferably from 1:2 to 1:4 Å. In a specific example, the antimicrobial composition comprises N-methyl-1,2-benzisothiazolin-3-enone and phenoxyethanol. Preferably, N-mercapto-1,2-benzisothiazolin The weight ratio of _3_keto-phenoxyethanol is from 1:120 to 1:16 〇. In one embodiment of the invention, the antimicrobial composition comprises N-mercapto-1,2-benzisothiazolin-3-enone and linseed guanidiniumpropyl propylene glycol-vaporized decyl ammonium phosphate. Preferably, the weight ratio of N_methyl_i,2_benzisothiazoline_3_one to linseed oil-coated propyl propylene glycol _ gasified dimethyl ammonium can vinegar is from 1:3 to 1: 400. In one embodiment of the invention, the antimicrobial composition comprises N-methyl-1,2-benzisothiazolinone and coconut oil amidinopropyl propylene glycol-vaporized decyl ammonium phosphate. Preferably, the weight ratio of N_mercapto-indole, 2_benzisothiazoline_3_one to coconut oil decyl propyl propylene glycol _ gasified dimethyl ammonium phosphate is from 1:0.1 to 1:125. The best is from k〇.4 to 1:125. In one embodiment of the present invention, the antibacterial composition comprises N-mercapto-1,2-benzisothiazolin-3-one and sorbic acid or a salt thereof, preferably potassium sorbate. Preferably, the weight ratio of 'N-methyl-1,2·benzisothiazoline _3-ketone to sorbic acid or a salt thereof is from 1:5 to 1:6 Torr, more preferably from 1:8. Until 1:6. In one embodiment of the invention, the antimicrobial composition comprises Ν_93646D17 13 201210506 methyl-1,2-benzisothiazoline-3-one and propyl paraben. Preferably, the weight ratio of N-methyl·1,2-benzisothiazoline _3-ketone to propylparaben is from 1:10 to 1:1200', more preferably from 1:13 to 1:12. In one embodiment of the present invention, the antibacterial composition comprises Ν-methyl-1,2-benzisothiazolin-3-enone and cis-s(3-chloroallyl)·3,5,7 Aza-1-nitrogen adamantane chloride. Preferably, Ν_methyl", benzoisothiazepine 3 嗣 cis-1_(3_ gas propyl)·3,5,7_triaza·ι_ rust rust diamond The weight ratio is from 1:0.2 to 1:400, more preferably from 1:0.3 to 1:200. In one embodiment of the invention, the antimicrobial composition comprises Ν·methyl-1,2-benzene. And isothiazoline-3-yl ketone and dehydroacetic acid or a salt thereof, preferably sodium dehydroacetate. Preferably, the weight of Ν_methylbenzisothiazoline -3- ketone to dehydroacetic acid or a salt thereof The ratio is from 1:1 to 1:5. In one embodiment of the invention, the antimicrobial composition comprises Ν-methyl-1,2-benzisothiazolin-3-enone and benzoic acid or a salt thereof Preferably, the weight ratio of sodium benzoate is preferably 'Ν-methyl-oxime, 2-benzoisoindole-p-benzoic acid or a salt thereof is from I] to 1::, more preferably From 1:4 to 1:: In one embodiment of the invention, the antimicrobial composition comprises Ν-methyl-1,2·benzisothiazoline-3-one and citric acid or a salt thereof, Preferably, the sodium citrate is relatively ground, and the weight ratio of Ν-曱基·ι, 2_benzoxanthene _3·嗣 to the rubic acid or its salt is from 1:30 to 1:2500, more preferably From 1:33 to 1 In one embodiment of the present invention, the antibacterial composition comprises Ν-methyl 1,2, and a sulphur _3 ketone and η ratio sulphur-zinc. Preferably, Ν_ The weight ratio of methyl-1,2-benzisothiazoline-3-one to zinc pyrithione is from 93646D17 14 201210506 1:0.01 to 1:6, more preferably from 1:4 to 1:4. The antibacterial agent in the composition of the present invention can be used "in its own form." Suitable solvents include: first water 'one yeast such as ethyl alcohol, propylene glycol, diethylene glycol glycol and polypropylene glycol; glycol monol, poly, private, such as methanol, ethanol, hydrazine, phenylethyl alcohol and benzene Oxypropanol...such as propyl and methyl ethyl; for example, ethyl acetate, butyl acetate, triglyceride and triacetin? Sour vinegar, such as carbonic acid propylene and dimethyl carbonate; and a mixture of the above solvents. Preferably, the solvent is selected from the group consisting of water, glycol, biaxial, and a mixture of various solvents. Suitable solids _ include, for example, dioxane (tetra), soil, strontium, cellulosic materials, gold and family of soil (eg, sodium, potassium, potassium) metal salts (eg, chloride, sulphate, telluride) , sulphate) and charcoal. When the antimicrobial component is formulated in a solvent, the formulation may optionally contain an intervening active agent. When this formulation contains a surfactant, it is typically an emulsive concentrate, an emulsion, a microemulsive concentrate, or a microemulsion. The emulsified concentrate forms an emulsion immediately after the addition of a sufficient amount of water. The microemulsified concentrate forms a microemulsion immediately after the addition of a sufficient amount of water. Such emulsified concentrates or microemulsion concentrates are generally known in the art; such formulations preferably contain no surfactant. General and specific details of further preparation of various microemulsions and microemulsified concentrates can be found in U.S. Patent No. 5,444,078. The antibacterial component can also be formulated in the form of a dispersion. The solvent component of the dispersion may be an organic solvent or water, preferably water. Such dispersions may contain adjuvants, 15 93646D17 201210506 eg, co-solvents, thickeners, antifreezes, dispersants, filled phthalocyanines, surfactants, biodispersants, continued Acid succinate, Ι 、, ° ° ° South _, multivalent cations, stabilizers, scale inhibitors and anti-corrosion additives. When the two antibacterial agents are each formulated with a solvent, the solvent used in the first antibacterial agent may be the same as or different from the solvent used to formulate another commercially available antibacterial agent. However, the application to most industrial antibiotics. The water system is better. Preferably, the two solvents are miscible. Those skilled in the art will appreciate that the antibacterial ingredients of the present invention may be added to the location before, at the same time, or prior to addition to the location. Preferably, the first antibacterial agent and the second antibacterial agent are added to the same location one after the other. When the antibacterial agents are added simultaneously or sequentially, the individual ingredients may each contain an adjuvant such as, for example, a solvent, a thickener, an anti-shacking agent, a coloring agent, a binding agent (such as ethylenediaminetetraacetic acid, ethylene). Amine disuccinic acid, imine disuccinic acid and salts thereof, dispersant, surfactant, biodispersant, acid succinate, decene, furanone, polyvalent cation, stabilizer, scale inhibitor and Antiseptic #Additive. The antibacterial composition of the present invention can be used to inhibit microorganisms or higher aquatic organisms (such as protozoa, by introducing an antibacterial effective amount of the composition above, inside or at the location where the microorganism is attacked). Growth of invertebrates, bryozoans, dinoflagellates, clams, mollusks, etc.). Suitable locations include, for example: industrial water; electrocoat deposition system; cooling tower; air cleaner; gas scrubber; pulp; wastewater treatment; decorative fountain; reverse osmosis 16 93646D17 201210506 filtration; (ultrafiltration); ballast water; evaporative condenser; heat exchanger; pulp and paper solution and additives; starch; plastic; emulsion; dispersant; pigment; milk; paint, such as varnish; construction products, such as cement , caulking paste and sealant 'built table adhesives' such as ceramic adhesives, carpet backing adhesives and composite adhesives; industrial or consumer adhesives; photographic chemicals; printing liquids; household items, such as bathrooms and Mill cleaning products; beauty products; cosmetics; shampoo; soap; detergent; industrial cleaners 'floor light' 72, laundry room washing water, metal processing liquid; conveyor belt lubricating fluid; hydraulic liquid (hydraulic Fluid);leather and leather products; textiles 'textile products; wood and wood products' such as plywood, chipboard, wallboard Fakeboard, laminated beam, oriented granule plate, hard plate and granule plate; petroleum refining liquid; fuel; oil field liquid, such as injection water, fissure liquid and drilling mud; agricultural adjuvant preservation\interface activity Preservation; medical equipment; diagnostic reagent storage; food preservation, such as plastic or paper food coating; pasteurizer for food, beverage and industrial procedures; toilet; recreational water; pond; and mineral spring. Preferably, the antibacterial composition of the present invention is for inhibiting the growth of microorganisms at a position selected from one or more of the following: mineral packing; papermaking solution and additives; starch; emulsion; dispersing agent; pigment; Coatings; building adhesives, such as ceramics, carpet backings; photographic chemicals; printing liquids; household items, such as bathroom and kitchen cleaning products; beauty 1: two: products; shampoo; fertilizer; Agent; industrial cleaner; agricultural two laundry room washing water; metal processing liquid; textile products; agent preservation; surfactant preservation; diagnostic reagent preservation; food insurance 93646D17 17 201210506 deposit; food, beverage and industrial procedures Sterilizer. The precise amount of the composition of the invention required to inhibit or control the growth of microorganisms and higher aquatic organisms at the location depends on the particular location to be protected. Typically, if the composition of the present invention provides from 0.1 to 10,000 ppm of the isoindole component of the composition at the location, the amount of the composition is sufficient to control the growth of the microorganism at that location. The isothiazolene of the composition is preferably present in an amount of at least ppm 5 ppm, more preferably at least 4 ppm, and most preferably at least i 〇 ppm. The amount of the isothiazoline component of the composition present at this position is preferably not more than 1000 ρ ηη, more preferably not more than 500 ppm, and most preferably not more than 2 〇〇 ppm. In one embodiment of the invention, the composition is substantially free of an enzymatic antibiotic. Preferably, when BBIT or MBIT is combined with one of decyl benzoate or ethyl p-hydroxybenzoate, the composition is substantially free of an enzymatic antibiotic. An enzymatic biocide is an enzyme having an antimicrobial activity, which is defined, for example, in U.S. Patent Application Publication No. 2002/0028754. (Examples) Materials and Methods The synergy of the combinations of the present invention was verified by examining a wide range of compound concentrations and ratios. A synergistic measurement system Kull, FC; Eisman, PC; Sylwestrowicz, HD and Mayer, RL is described in Applied Microbiology 9: 538-541 (1961), an industrially accepted method, which is based on the following formula: 93646D17 201210506 proportion:

Qa’QA + Qb/QB =協同指數(Synergy Index, “SI”）其中：Qa’QA + Qb/QB = Synergy Index ("SI") where:

Qa=化合物A(第一種成分）單獨作用的ppm濃度，其產生終點（化合物A的MIC)。Qa = ppm concentration of Compound A (first component) acting alone, which produces an endpoint (MIC of Compound A).

Qa=化合物A於混合物中的ppm濃度，其產生終點。 Qb=化合物B(第二種成分)單獨作用的ppm濃度，其產生終點（化合物B的MIC)。Qa = ppm concentration of Compound A in the mixture, which produces an endpoint. Qb = ppm concentration of Compound B (second component) acting alone, which produces an endpoint (MIC of Compound B).

Qb=化合物B於混合物中的ppm濃度，其產生終點。當Qa/QA和Qb/QB之總和大於1，表示拮抗作用。當 Qa/QA和QiAJb之總和等於1，表示力口乘作用，而當少於1，表示協同作用。SI越低，顯示此特定混合物協同作用越大。抗菌劑之最小抑制濃度(MIC)為於特定一組條件下試驗出能夠P方止所添加微生物之生長的最低濃度。協同試驗使用標準微量滴定盤分析試驗（standard microtiter plate assay)以設計為最適所試驗微生物生長之培養基進行。補充0.2%葡萄糖及0.1%酵母菌萃取物之最小鹽類培養基(M9GY培養基)係用於試驗細菌；馬鈴薯葡萄糖液體培養基(PDB培養基)係用於試驗酵母菌及黴菌。於此方法中，抗菌劑與其他個人護理原料之廣範圍的組合係於各種濃度之BBIT或MBIT的存在下’藉由進行高解析MIC分析試驗而予以測試。高解析MIC係藉由添加不同量之抗菌劑至微滴定盤之一行’然後以自動液體處理系 93646D17 19 201210506 統進行十倍序列稀釋，以獲得自2ppm至，刪卩㈣範圍之活性成分之一系列終點而予以測定。本發明之組合之協同作用係取決於抗細菌(大腸桿菌 (五.co/卜ATCC#8739))、酵母菌（念珠菌（c⑽化廳_ATCC 10231))、及黴菌（黑趟黴μi6彻)）。細菌係以每mL 5xl〇6細菌之濃度使用，而酵母菌和黴菌係以每 mL5xl0真菌。此等微生物係代表於許多消費性及工業應用中之自然污染物。培養盤於坑（酵母菌及㈣）或3〇。〇 (細菌）在經過各種培養時間後經目視評估微生物的生長 (混濁）以判定MIC。. 本發明BBIT組合之協同作用之驗證試驗結果顯示於下表1至26。於每-試驗，第一種成分⑷為bbit而第二種成分⑻為其他商業可得之抗_。每—表格顯示ββιτ 及第二種成分之特定組合；經培養時間對抗所檢測之微生 ^之結果；以ppm表示之終點活性，其係藉由量測ββιτ 單用（Qa)、第二種成分單用（Qb)、ΒΒΙΤ於鹿.合物中（Qa)、及第二種成分於混合物中（Qb)之MIC而得；所計算之幻值；及所測試之每-組合_T/第二種成分或趟)的協同比率之範圍。；：本發明ΜΒΓΓ組合之協同作用之驗證試驗結果顯示於下表27至54。於每-試驗，第一種成分（α)為臟τ而第二種成分⑻為其他商f可得之抗_。每—表格顯示贿T及第二種成分之特定組合；經培養時間對抗所檢測之微生物之結果；以ppm表示之終點活性，其係藉由量測 93646D17 20 201210506 MBIT單用（QA)、第二種成分單用（QB)、MBIT於混合物中 (Qa)、及第二種成分於混合物中（Qb)之MIC而得；所計算之SI值；及所測試之每一組合(MBIT/第二種成分或A/B) 的協同比率之範圍。 21 93646D17 201210506 表1 第一種成分(A)=N-(正-丁基)-1，2-苯并異噻唑啉酮(ΒΒΙΤ) 第二種成分(Β)=氣化苯二甲烴銨（海亞敏(Hyamine) 3 500) 微生物 Qa Qb SI A/B E. coli 8739 M9GY 0 3 1.00 (48小時） 16.667 40 1.00 1/2 10 50 1.00 1/5 12.5 60 1-00 1/5 100 0 1.00 微生物 Q. Qb SI A/B G albicans 10231 - PDB 0 60 1.00 (24小時） 2 4 0.40 1/2 2 5 0.42 1/3 2 6 0.43 1/3 2 8 0.47 1/4 2 10 0.50 1/5 2 20 0.67 1/10 2 30 0.83 1/15 4 1 0.68 1/0.3 4 2 0.70 1/0.5 4 3 0.72 1/0.8 4 4 0.73 1/1 4 5 0.75 1/1 4 6 0.77 1/2 4 8 0.80 1/2 4 10 0.83 1/3 7.5 0 1.00 微生物 Q- Qb SI A/B A niger 16404 * PDB 0 200 1.00 (7天） 5 60 0.55 1/12 5 80 0.65 1/16 5 100 0.75 1/20 10 50 0.75 1/5 10 60 0.80 1/6 10 80 0.90 1/8 15 4 0.77 1/0.3 15 5 0.78 1/0.3 22 93646D17 201210506 微生物 Qa Qb SI A/B 15 6 0.78 1/0.4 15 8 0.79 1/0.5 15 10 0.80 1/0.7 15 20 0.85 1/1 15 30 0.90 1/2 20 0 1.00 所測之ΒΒΙΤ/氯化苯二甲烴銨之比率範圍自1/0.02至1/500。 23 93646D17 201210506 表2 第一種成分(A)=N-(正-丁基)-1，2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=氣化苯銨松寧（海亞敏1622) 微生物 Qa Qb SI A/B E. C07/8739 - M9GY 0 20 1.00 (72小時） 40 6 0.70 1/0.2 40 8 0.80 1/0.2 40 10 0.90 1/0.3 50 3 0.65 1/0.06 50 4 0.70 1/0.08 50 5 0.75 1/0.1 50 6 0.80 1/0.1 50 8 0.90 1/0.2 60 1 0.65 1/0.02 60 2 0.70 1/0.03 60 3 0.75 1/0.05 60 4 0.80 1/0.07 60 5 0.85 1/0.08 60 6 0.90 1/0.1 70 4 0.90 1/0.06 100 0 1.00 微生物 Q. 0» SI A/B C. albicans 10231 · PDB 0 4 1.00 (24小時） 4 1 0.92 1/0.3 6 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 * PDB 0 10 1.00 (7天） 5 4 0.65 1/0.8 5 5 0.75 1/0.8 5 6 0.85 1/1 10 4 0.90 1/0.4 20 0 1.00 所測之BBIT/氯化苯銨松寧之比率範圍自1/0.02至1/500。 24 93646D17 201210506 表3 第一種成分(A)=N-(正-丁基)-1，2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=苯甲醇微生物 Qa Qb SI AfB E. ¢£7//8739 - M9GY 0 2000 1.00 CM小時） 100 2000 0.90 1/20 200 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 3000 1.00 (48小時） 2.5 600 0.45 1/240 2.5 800 0.52 1/320 2.5 1000 0.58 1/400 2.5 2000 0.92 1/800 5 400 0.63 1/80 5 500 0.67 1/100 5 600 0.70 1/120 5 800 0.77 1/160 5 1000 0.83 1/200 7.5 20 0.76 1/3 7.5 30 0.76 1/4 7.5 40 0.76 1/5 7.5 50 0.77 1/7 7.5 60 0.77 1/8 7.5 80 0.78 1/11 7.5 100 0.78 1/13 7.5 200 0.82 1/27 7.5 300 0.85 1/40 7.5 400 0.88 1/53 7.5 500 0.92 1/67 7.5 600 0.95 1/80 10 0 1.00 -… 微生物 Qa Qb SI A/B A. niger 16404 * PDB 0 5000 1.00 (3天） 5 5000 1.13 1/1000 10 5000 1.25 1/500 15 4000 1.18 1/267 25 93646D17 201210506 微生物 Qa Qb SI A/B 20 5000 1.50 1/250 40 0 1.00 — 所測之ΒΒΙΤ/苯甲醇之比率範圍自1/0.2至1/5000。 26 93646D17 201210506 表4 第一種成分(A)=N-(正-丁基)-1,2-苯并異噻唑啉酮（BBIT) 第二種成分(B)=苯并異噻唑啉酮(BIT) 微生物 Q- Qb SI A/B K coil 8739 · M9GY 0 8 1.00 (72小時） 40 1 0.53 1/0.03 40 2 0.65 1/0.05 40 3 0.78 1/0.08 40 4 0.90 1/0.1 50 1 0.63 1/0.02 50 2 0.75 1/0.4 50 3 0.88 1/0.06 60 1 0.73 1/0.02 60 2 0.85 1/0.03 100 0 1.00 — 微生物 Q. Qb SI A/B C. albicans 10231 PDB 0 30 1.00 (48小時） 2.5 8 0.60 1/3. 2.5 10 0.67 1/4 5 3 0.77 1/0.6 5 4 0.80 1/0.8 5 5 0.83 1/1 5 6 0.87 1/1.2 5 8 0.93 1/1.6 7.5 0 1.00 …- 微生物 Qa Qb SI Am A. niger\Q4Q^ - PDB 0 100 1.00 (7天） 5 80 0.86 1/16 10 20 0.33 1/2 10 30 0.43 1/3 10 40 0.53 1/4 10 50 0.63 1/5 10 60 0.73 1/6 10 80 0.93 1/8 15 8 0.27 1/0.5 15 10 0.29 1/0.7 15 20 0.39 1/1 27 93646D17 201210506 微生物 Qa Qb SI A/B 15 30 0.49 1/2 15 40 0.59 1/3 15 50 0.69 1/3 15 60 0.79 1/4 20 10 0.35 1/0.5 20 20 0.45 1/1 20 30 0.55 1/2 20 40 0.65 1/2 20 50 0.75 1/3 20 60 0.85 1/3 40 2 0.52 1/0.05 . 40 3 0.53 1/0.08 40 4 0.54 1/0.1 40 5 0.55 1/0.1 40 6 0.56 1/0.1 40 8 0.58 1/0.2 40 10 0.60 1/0.3 40 20 0.70 1/0.5 40 30 0.80 1/0.8 40 40 0.90 1/1 80 0 1.00 — 所測之BBIT/苯并異噻唑啉酮之比率範圍自1/0.02至 1/500。 28 93646D17 201210506 表5 第一種成分(A)=N-(正-丁基)-1,2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=2-溴-2-硝基丙烷-1，3-二醇(BNPD) 微生物〇ϋ si E. coZ? 8739 - M9GY (48小時） οοοοο ο ο ο ο οοοο ο 4444 4 5 5 5 5778 ο 微生物 C. albicans 10231 - PDB (48小時）Qb = ppm concentration of Compound B in the mixture, which produces an endpoint. When the sum of Qa/QA and Qb/QB is greater than 1, it indicates antagonism. When the sum of Qa/QA and QiAJb is equal to 1, it means that the force is multiplied, and when it is less than 1, it means synergy. The lower the SI, the greater the synergy of this particular mixture. The minimum inhibitory concentration (MIC) of the antimicrobial agent is the lowest concentration at which a P group can be tested for growth of the added microorganism under a specific set of conditions. The synergistic assay was performed using a standard microtiter plate assay designed to optimize the growth of the microorganisms tested. The minimum salt medium (M9GY medium) supplemented with 0.2% glucose and 0.1% yeast extract was used to test bacteria; potato glucose liquid medium (PDB medium) was used to test yeasts and molds. In this method, a wide range of combinations of antimicrobial agents and other personal care ingredients are tested in the presence of various concentrations of BBIT or MBIT' by performing high resolution MIC analysis tests. The high-resolution MIC is prepared by adding different amounts of antibacterial agent to one of the microtiter plates and then performing 10-fold serial dilution with the automatic liquid treatment system 93646D17 19 201210506 to obtain one of the active ingredients ranging from 2 ppm to (4). The end point of the series was determined. The synergy of the combination of the present invention depends on the antibacterial (E. coli (f. co/b ATCC #8739)), the yeast (Candida (c(10) chemistry hall_ATCC 10231)), and the mold (Black sputum μi6 )). The bacteria were used at a concentration of 5 x 1 细菌6 bacteria per mL, while the yeast and mold lines were 5 x 10 fungi per mL. These microorganisms represent natural contaminants in many consumer and industrial applications. The plate is incubated in the pit (yeast and (4)) or 3 〇. 〇 (bacteria) The growth (turbidity) of the microorganism was visually evaluated after various culture times to determine the MIC. The results of the verification test for the synergy of the BBIT combinations of the present invention are shown in Tables 1 to 26 below. In each test, the first component (4) is bbit and the second component (8) is other commercially available anti-. Each table shows the specific combination of ββιτ and the second component; the result of the culture time against the detected micro-production; the end-activity in ppm, which is measured by ββιτ alone (Qa), the second Ingredients used alone (Qb), in the deer compound (Qa), and in the second component in the mixture (Qb); calculated magic value; and each combination tested _T/ The range of synergistic ratios of the second component or 趟). ;: The results of the verification test for the synergistic effect of the combination of the present invention are shown in Tables 27 to 54 below. In each test, the first component (α) is the dirty τ and the second component (8) is the other com. Each table shows the specific combination of Bribe T and the second component; the result of the culture time against the microorganisms tested; the endpoint activity expressed in ppm by measuring 93646D17 20 201210506 MBIT Single Use (QA), The two components are used alone (QB), MBIT in the mixture (Qa), and the MIC of the second component in the mixture (Qb); the calculated SI value; and each combination tested (MBIT/ The range of synergistic ratios for the two components or A/B). 21 93646D17 201210506 Table 1 The first component (A) = N-(n-butyl)-1,2-benzisothiazolinone (ΒΒΙΤ) The second component (Β) = gasified phthalic ammonium salt (Hyamine 3 500) Microorganism Qa Qb SI A/B E. coli 8739 M9GY 0 3 1.00 (48 hours) 16.667 40 1.00 1/2 10 50 1.00 1/5 12.5 60 1-00 1/5 100 0 1.00 Microbial Q. Qb SI A/BG albicans 10231 - PDB 0 60 1.00 (24 hours) 2 4 0.40 1/2 2 5 0.42 1/3 2 6 0.43 1/3 2 8 0.47 1/4 2 10 0.50 1/ 5 2 20 0.67 1/10 2 30 0.83 1/15 4 1 0.68 1/0.3 4 2 0.70 1/0.5 4 3 0.72 1/0.8 4 4 0.73 1/1 4 5 0.75 1/1 4 6 0.77 1/2 4 8 0.80 1/2 4 10 0.83 1/3 7.5 0 1.00 Microbial Q- Qb SI A/BA niger 16404 * PDB 0 200 1.00 (7 days) 5 60 0.55 1/12 5 80 0.65 1/16 5 100 0.75 1/ 20 10 50 0.75 1/5 10 60 0.80 1/6 10 80 0.90 1/8 15 4 0.77 1/0.3 15 5 0.78 1/0.3 22 93646D17 201210506 Microorganism Qa Qb SI A/B 15 6 0.78 1/0.4 15 8 0.79 1/0.5 15 10 0.80 1/0.7 15 20 0.85 1/1 15 30 0.90 1/2 The ratio of bismuth/chlorinated dimethylammonium chloride measured from 20 0 1.00 ranges from 1/0.02 to 1/500. 23 93646D17 201210506 Table 2 The first component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) The second component (B) = gasified benzyl ammonium Songning ( Hai Yamin 1622) Microorganism Qa Qb SI A/B E. C07/8739 - M9GY 0 20 1.00 (72 hours) 40 6 0.70 1/0.2 40 8 0.80 1/0.2 40 10 0.90 1/0.3 50 3 0.65 1/0.06 50 4 0.70 1/0.08 50 5 0.75 1/0.1 50 6 0.80 1/0.1 50 8 0.90 1/0.2 60 1 0.65 1/0.02 60 2 0.70 1/0.03 60 3 0.75 1/0.05 60 4 0.80 1/0.07 60 5 0.85 1/0.08 60 6 0.90 1/0.1 70 4 0.90 1/0.06 100 0 1.00 Microorganism Q. 0» SI A/B C. albicans 10231 · PDB 0 4 1.00 (24 hours) 4 1 0.92 1/0.3 6 0 1.00 Microorganism Qa Qb SI A/B A. niger 16404 * PDB 0 10 1.00 (7 days) 5 4 0.65 1/0.8 5 5 0.75 1/0.8 5 6 0.85 1/1 10 4 0.90 1/0.4 20 0 1.00 The ratio of BBIT/ammonium chloride chlorinated from 1/0.02 to 1/500. 24 93646D17 201210506 Table 3 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = benzyl alcohol microorganism Qa Qb SI AfB E. 7£7//8739 - M9GY 0 2000 1.00 CM hours) 100 2000 0.90 1/20 200 0 1.00 Microbial Qa Qb SI A/B C. albicans 10231 - PDB 0 3000 1.00 (48 hours) 2.5 600 0.45 1/ 240 2.5 800 0.52 1/320 2.5 1000 0.58 1/400 2.5 2000 0.92 1/800 5 400 0.63 1/80 5 500 0.67 1/100 5 600 0.70 1/120 5 800 0.77 1/160 5 1000 0.83 1/200 7.5 20 0.76 1/3 7.5 30 0.76 1/4 7.5 40 0.76 1/5 7.5 50 0.77 1/7 7.5 60 0.77 1/8 7.5 80 0.78 1/11 7.5 100 0.78 1/13 7.5 200 0.82 1/27 7.5 300 0.85 1/40 7.5 400 0.88 1/53 7.5 500 0.92 1/67 7.5 600 0.95 1/80 10 0 1.00 -... Microorganism Qa Qb SI A/B A. niger 16404 * PDB 0 5000 1.00 (3 days) 5 5000 1.13 1 /1000 10 5000 1.25 1/500 15 4000 1.18 1/267 25 93646D17 201210506 Microbial Qa Qb SI A/B 20 5000 1.50 1/250 40 0 1.00 — The measured ratio of hydrazine/benzyl alcohol ranges from 1/0.2 to 1 /5000. 26 93646D17 201210506 Table 4 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = benzisothiazolinone ( BIT) Microbial Q- Qb SI A/BK coil 8739 · M9GY 0 8 1.00 (72 hours) 40 1 0.53 1/0.03 40 2 0.65 1/0.05 40 3 0.78 1/0.08 40 4 0.90 1/0.1 50 1 0.63 1/ 0.02 50 2 0.75 1/0.4 50 3 0.88 1/0.06 60 1 0.73 1/0.02 60 2 0.85 1/0.03 100 0 1.00 — Microorganism Q. Qb SI A/B C. albicans 10231 PDB 0 30 1.00 (48 hours) 2.5 8 0.60 1/3. 2.5 10 0.67 1/4 5 3 0.77 1/0.6 5 4 0.80 1/0.8 5 5 0.83 1/1 5 6 0.87 1/1.2 5 8 0.93 1/1.6 7.5 0 1.00 ...- Microbial Qa Qb SI Am A. niger\Q4Q^ - PDB 0 100 1.00 (7 days) 5 80 0.86 1/16 10 20 0.33 1/2 10 30 0.43 1/3 10 40 0.53 1/4 10 50 0.63 1/5 10 60 0.73 1/6 10 80 0.93 1/8 15 8 0.27 1/0.5 15 10 0.29 1/0.7 15 20 0.39 1/1 27 93646D17 201210506 Microorganism Qa Qb SI A/B 15 30 0.49 1/2 15 40 0.59 1/3 15 50 0.69 1/3 15 60 0.79 1/4 20 10 0.35 1/0.5 20 20 0.45 1/1 20 30 0.55 1/2 20 40 0.65 1/2 20 50 0.75 1/3 20 60 0.85 1/3 40 2 0.52 1/0.05 . 40 3 0.53 1/0.08 40 4 0.54 1/0.1 40 5 0.55 1/0.1 40 6 0.56 1 /0.1 40 8 0.58 1/0.2 40 10 0.60 1/0.3 40 20 0.70 1/0.5 40 30 0.80 1/0.8 40 40 0.90 1/1 80 0 1.00 — Ratio of BBIT/benzisothiazolinone measured From 1/0.02 to 1/500. 28 93646D17 201210506 Table 5 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = 2-bromo-2-nitrate Propane-1,3-diol (BNPD) microbial 〇ϋ si E. coZ? 8739 - M9GY (48 hours) οοοοο ο ο ο ο οοοο ο 4444 4 5 5 5 5778 ο Microorganism C. albicans 10231 - PDB (48 hour)

Λ Q 0 2 4 4 4 4 4 4 4 4 4 4 6 6 6 10 1.00 1 0.50 1/0.03 2 0.60 1/0.05 3 0.70 1/0.08 4 0.80 1/0.1 5 0.90 1/0.1 1 0.60 1/0.02 2 0.70 1/0.04 3 0.80 1/0.06 4 0.90 1/0.08 1 0.80 1/0.01 2 0.90 1/0.03 1 0.90 1/0.01 0 1.00 —— 1 SI ΑίΒ 2000 1.00 1000 0.75 1/500 20 0.51 1/5 30 0.52 1/8 40 0.52 1/10 50 0.53 1/13 60 0.53 1/15 80 0.54 1/20 100 0.55 1/25 200 0.60 1/50 300 0.65 1/75 400 0.70 1/100 500 0.75 1/125 600 0.80 1/150 800 0.90 1/200 1 0.75 1/0.2 2 0.75 1/0.3 3 0.75 1/0.5 29 93646D17 201210506 微生物 Q. Qb SI A/B 6 4 0.75 1/0.7 6 5 0.75 1/0.8 6 6 0.75 1/1 6 8 0.75 1/1 6 10 0-76 1/2 6 20 0.76 1/3 6 30 i 0.77 1/5 6 40 0.77 1/7 6 50 0.78 1/8 6 60 0.78 1/10 6 80 0.79 1/13. 6 100 0.80 1/17 6 200 0.85 1/33 6 300 0.90 1/50 8 0 1.00 — 微生物 Q. Qb SI A/B A 16404 - PDB 0 2000 1.00 (7天） 5 1000 -0,75 1/200 10 8 ..0.50 1/0.8 10 10 > 0.51 1/1 10 20 0.51 1/2 10 30 ;0.52 1/3 10 40 0.52 1/4 10 50 0.53 1/5 10 60 0.53 1/6 10 80 0.54 1/8 10 100 0.55 1/10 10 200 0.60 1/20 10 300 0.65 1/30 10 400 0.70 1/40 10 500 0.75 1/50 10 600 0.80 1/60 10 800 0.90 1/80 20 0 , 1.00 — 所測之BBIT/2-漠-2-硝基丙烷-1，3-二醇之比率範圍自 1/0.01 至 1/500。 30 93646D17 201210506 表6 第一種成分(A)=N-(正-丁基)-1,2-苯并異噻唑啉酮(BBIT) 第二種成分(B)= 丁二醇微生物 Q. Qb SI A/B E. co7i8739 - M9GY 0 20000 1.00 (48小時） 40 5000 0.65 1/125 40 6000 0.70 1/150 40 8000 0.80 1/200 40 10000 0.90 1/250 50 2000 0.60 1/40 50 3000 0.65 1/60 50 4000 0.70 1/80 50 5000 0.75 1/100 50 6000 0.80 1/120 50 8000 0.90 1/160 70 2000 0.80 1/29 70 3000 0.85 1/43 70 4000 0.90 1/57 80 200 0.81 1/3 80 300 0.82 1/4 80 400 0.82 1/5 80 500 0.83 1/6 80 600 0.83 1/8 80 800 0.84 1/10 80 1000 0.85 1/13 80 2000 0.90 1/25 100 0 1.00 …· 微生物 Q· Qb SI A/B C· albicans 10231 · PDB 0 20000 1.00 (24小時） 2 10000 0.83 1/5000 4 200 0.68 1/50 4 300 0.68 1/75 4 400 0.69 1/100 4 500 0.69 1/125 4 600 0.70 1/150 4 800 0.71 1/200 4 1000 0.72 1/250 31 93646D17 201210506 微生物 Q. Qb SI Am 4 2000 0.77 1/500 4 3000 0.82 1/750 4 4000 0.87 1/1000 4 5000 0.92 1/1250 6 0 1.00 — 微生物 Q. Qb SI A/B A. niger 16404 - PDB 0 10000 1.00 ---- (3天） 5 5000 0.83 5 6000 0.93 15 0 1.00 所測之BBIT/丁二醇之比率範圍自1/0.2至1/5000。 32 93646D17 201210506 表7 第一種成分(A)=N-(正-丁基)-1,2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=辛醯二醇Λ Q 0 2 4 4 4 4 4 4 4 4 4 4 6 6 6 10 1.00 1 0.50 1/0.03 2 0.60 1/0.05 3 0.70 1/0.08 4 0.80 1/0.1 5 0.90 1/0.1 1 0.60 1/0.02 2 0.70 1/0.04 3 0.80 1/0.06 4 0.90 1/0.08 1 0.80 1/0.01 2 0.90 1/0.03 1 0.90 1/0.01 0 1.00 —— 1 SI ΑίΒ 2000 1.00 1000 0.75 1/500 20 0.51 1/5 30 0.52 1/8 40 0.52 1/10 50 0.53 1/13 60 0.53 1/15 80 0.54 1/20 100 0.55 1/25 200 0.60 1/50 300 0.65 1/75 400 0.70 1/100 500 0.75 1/125 600 0.80 1/150 800 0.90 1/200 1 0.75 1/0.2 2 0.75 1/0.3 3 0.75 1/0.5 29 93646D17 201210506 Microorganism Q. Qb SI A/B 6 4 0.75 1/0.7 6 5 0.75 1/0.8 6 6 0.75 1 /1 6 8 0.75 1/1 6 10 0-76 1/2 6 20 0.76 1/3 6 30 i 0.77 1/5 6 40 0.77 1/7 6 50 0.78 1/8 6 60 0.78 1/10 6 80 0.79 1/13. 6 100 0.80 1/17 6 200 0.85 1/33 6 300 0.90 1/50 8 0 1.00 — Microorganism Q. Qb SI A/BA 16404 - PDB 0 2000 1.00 (7 days) 5 1000 -0,75 1/200 10 8 ..0.50 1/0.8 10 10 > 0.51 1/1 10 20 0.51 1/2 10 30 ; 0.52 1/3 10 40 0.5 2 1/4 10 50 0.53 1/5 10 60 0.53 1/6 10 80 0.54 1/8 10 100 0.55 1/10 10 200 0.60 1/20 10 300 0.65 1/30 10 400 0.70 1/40 10 500 0.75 1 /50 10 600 0.80 1/60 10 800 0.90 1/80 20 0 , 1.00 — The ratio of BBIT/2-involved 2-nitropropane-1,3-diol measured ranges from 1/0.01 to 1/ 500. 30 93646D17 201210506 Table 6 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = butanediol microorganism Q. Qb SI A/B E. co7i8739 - M9GY 0 20000 1.00 (48 hours) 40 5000 0.65 1/125 40 6000 0.70 1/150 40 8000 0.80 1/200 40 10000 0.90 1/250 50 2000 0.60 1/40 50 3000 0.65 1 /60 50 4000 0.70 1/80 50 5000 0.75 1/100 50 6000 0.80 1/120 50 8000 0.90 1/160 70 2000 0.80 1/29 70 3000 0.85 1/43 70 4000 0.90 1/57 80 200 0.81 1/3 80 300 0.82 1/4 80 400 0.82 1/5 80 500 0.83 1/6 80 600 0.83 1/8 80 800 0.84 1/10 80 1000 0.85 1/13 80 2000 0.90 1/25 100 0 1.00 ...· Microorganism Q· Qb SI A/BC· albicans 10231 · PDB 0 20000 1.00 (24 hours) 2 10000 0.83 1/5000 4 200 0.68 1/50 4 300 0.68 1/75 4 400 0.69 1/100 4 500 0.69 1/125 4 600 0.70 1/150 4 800 0.71 1/200 4 1000 0.72 1/250 31 93646D17 201210506 Microorganism Q. Qb SI Am 4 2000 0.77 1/500 4 3000 0.82 1/750 4 4000 0.87 1/1000 4 5000 0.92 1/1250 6 0 1. 00 — Microbial Q. Qb SI A/B A. niger 16404 - PDB 0 10000 1.00 ---- (3 days) 5 5000 0.83 5 6000 0.93 15 0 1.00 The ratio of BBIT/butanediol measured ranges from 1/ 0.2 to 1/5000. 32 93646D17 201210506 Table 7 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = octanediol

微生物 Qa Qb SI A7B E. co；i 8739 - M9GY (24小時） 040404040405050507070708000 微生物 C. albicans 10231 PDB (24小時）Microorganism Qa Qb SI A7B E. co;i 8739 - M9GY (24 hours) 040404040405050507070708000 Microorganisms C. albicans 10231 PDB (24 hours)

a Q 2 4 4 4 6 6 6 6 6 6a Q 2 4 4 4 6 6 6 6 6 6

A. niger 16404 PDB 2000 1.00 400 0.60 1/10 500 0.65 1/13 600 0.70 1/15 800 0.80 1/20 1000 0.90 1/25 200 0.60 1/4 600 0.80 1/12 800 0.90 1/16 200 0.80 1/3 300 0.85 1/4 400 0.90 1/6 200 0.90 1/3 ' 0 1.00 ---- i SI AJB 3000 1.00 2000 0.92 1/1000 500 0.67 1/125 600 0.70 1/150 800 0.77 1/200 1000 0.83 1/250 40 0.76 1/7 50 0.77 1/8 60 0.77 1/10 80 0.78 1/13 100 0.78 1/17 200 0.82 1/33 300 0.85 1/50 400 0.88 1/67 500 0.92 1/83 0 1.00 3000 1.00 3 3 93646D17 201210506 微生物 Qa Qb SI AJB (7天） 5 1000 0.58 1/200 5 2000 0.92 1/400 10 1000 0.83 1/100 20 0 1.00 所測之ΒΒΙΤ/辛醯二醇之比率範圍自1/0.2至1/5000。 34 93646D17 201210506 表8 第一種成分(A)=N-(正-丁基)-l，2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=氯苯甘醚微生物 Q. Qb SI A/B E. 8739 - M9GY 0 2000 1.00 (24小時） 40 500 0.65 1/13 40 600 0.70 1/115 40 800 0.80 1/20 40 1000 0.90 1/25 50 300 0.65 1/6 50 400 0.70 1/8 50 500 0.75 1/10 50 600 0.80 1/12 50 800 0.90 1/16 70 200 0.80 1/3 70 300 0.85 1/4 70 400 0.90 1/6 80 100 0.85 1/1’ 80 200 0.90 1/3 100 0 1.00 微生物 Q. Qb SI A/B C. albicans 10231 - PDB 0 2000 1.00 (24小時） 2 800 0.65 1/400 2 1000 0.75 1/500 4 300 0.65 1/75 4 400 0.70 1/100 4 500 0.75 1/125 4 600 0.80 1/150 4 800 0.90 1/200 6 10 0.76 1/2 6 20 0.76 1/3 6 30 0.77 1/5 6 40 0.77 1/7 6 50 0.78 1/8 6 60 0.78 1/10 6 80 0.79 1/13 6 100 0.80 1/17 35 93646D17 201210506 微生物 Q- Qb SI A/B 6 200 0.85 1/33 6 300 0.90 1/50 8 0 1.00 微生物 Q. Qb SI A/B A. niger 16404 - PDB 0 2000 1.00 (4天） 5 1000 0.83 1/200 15 0 1.00 所測之ΒΒΙΤ/氯苯甘醚之比率範圍自1/0.2至1/5000。 36 93646D17 201210506 表9 第一種成分(A)=N-(正-丁基)-1,2-苯并異噻唑啉酮作31丁) 第二種成分(B)=DMDM 乙内醯脲(Hydantoin) (DMDMH)A. niger 16404 PDB 2000 1.00 400 0.60 1/10 500 0.65 1/13 600 0.70 1/15 800 0.80 1/20 1000 0.90 1/25 200 0.60 1/4 600 0.80 1/12 800 0.90 1/16 200 0.80 1 /3 300 0.85 1/4 400 0.90 1/6 200 0.90 1/3 ' 0 1.00 ---- i SI AJB 3000 1.00 2000 0.92 1/1000 500 0.67 1/125 600 0.70 1/150 800 0.77 1/200 1000 0.83 1/250 40 0.76 1/7 50 0.77 1/8 60 0.77 1/10 80 0.78 1/13 100 0.78 1/17 200 0.82 1/33 300 0.85 1/50 400 0.88 1/67 500 0.92 1/83 0 1.00 3000 1.00 3 3 93646D17 201210506 Microbial Qa Qb SI AJB (7 days) 5 1000 0.58 1/200 5 2000 0.92 1/400 10 1000 0.83 1/100 20 0 1.00 The ratio of ΒΒΙΤ/octyl diol measured ranges from 1/0.2 to 1/5000. 34 93646D17 201210506 Table 8 First component (A) = N-(n-butyl)-l,2-benzisothiazolinone (BBIT) Second component (B) = chlorphene ether microorganism Q. Qb SI A/B E. 8739 - M9GY 0 2000 1.00 (24 hours) 40 500 0.65 1/13 40 600 0.70 1/115 40 800 0.80 1/20 40 1000 0.90 1/25 50 300 0.65 1/6 50 400 0.70 1/8 50 500 0.75 1/10 50 600 0.80 1/12 50 800 0.90 1/16 70 200 0.80 1/3 70 300 0.85 1/4 70 400 0.90 1/6 80 100 0.85 1/1' 80 200 0.90 1 /3 100 0 1.00 Microbial Q. Qb SI A/B C. albicans 10231 - PDB 0 2000 1.00 (24 hours) 2 800 0.65 1/400 2 1000 0.75 1/500 4 300 0.65 1/75 4 400 0.70 1/100 4 500 0.75 1/125 4 600 0.80 1/150 4 800 0.90 1/200 6 10 0.76 1/2 6 20 0.76 1/3 6 30 0.77 1/5 6 40 0.77 1/7 6 50 0.78 1/8 6 60 0.78 1/10 6 80 0.79 1/13 6 100 0.80 1/17 35 93646D17 201210506 Microorganism Q- Qb SI A/B 6 200 0.85 1/33 6 300 0.90 1/50 8 0 1.00 Microbial Q. Qb SI A/B A. niger 16404 - PDB 0 2000 1.00 (4 days) 5 1000 0.83 1/200 15 The ratio of bismuth/chlorophenylglycol measured from 0 1.00 ranges from 1/0.2 to 1/5000. 36 93646D17 201210506 Table 9 The first component (A) = N-(n-butyl)-1,2-benzisothiazolinone as 31 butyl) The second component (B) = DMDM carbendazim ( Hydantoin) (DMDMH)

微生物 Qe Qb SI E. colj 8739 - M9GY 0 200 1.00 (72小時） 40 8 0.44 1/0.2 40 10 0.45 1/0.3 40 20 0.50 1/0.5 40 30 0.55 1/0.8 40 40 0.60 1/1 40 50 0.65 1/1 40 60 0.70 1/2 40 80 0.80 1/2 40 100 0.90 1/3 50 3 0.52 1/0.06 50 4 0.52 1/0.08 50 5 0.53 1/0.1 50 6 0.53 1/0.1 50 δ 0.54 1/0.2 50 10 0.55 1/0.2 50 20 0.60 1/0.4 50 30 0.65 1/0.6 50 40 0.70 1/0.8 50 50 0.75 . 1/1 50 60 0.80 1/1.2 50 80 0.90 1/1.6 60 6 0.63 1/0.1 60 8 0.64 1/0.1 60 10 0.65 1/0.2 60 20 0.70 1/0.3 60 30 0.75 1/0.5 60 40 0.80 1/0.7 60 50 0.85 1/0.8 60 60 0.90 1/1 80 8 0.84 1/0.1 80 10 0.85 1/0.1 80 20 0.90 1/0.3 37 93646D17 201210506 微生物 Qa Qb SI A/B 100 0 1.00 C. albicans 10231 PDB 0 3000 1.00 (24小時） 5 600 0.87 1/120 5 800 0.93 1/160 7.5 0 1.00 微生物 Qe Qb SI A/B A. niger 16404 - PDB 0 1000 1.00 (7天） 20 80 0.33 1/4 20 100 0.35 1/5 20 200 0.45 1/10 20 300 0.55 1/15 20 400 0.65 1/20 20 500 0.75 1/25 20 600 0.85 1/30 40 100 0.60 1/3 40 200 0.70 1/5 40 300 0.80 1/8 40 400 0.90 1/10 80 0 1.00 所測之ΒΒΠ7 DMDM乙内醯脲之比率範圍自1/0.2至 1/5000。 38 93646D17 201210506 表ίο 第一種成分(A)=N-(正-丁基)-1,2-苯并異噻唑啉酮(ΒΒΙΤ) 第二種成分(Β)=二硫基-2,2’-雙(Ν-甲基苯甲醯胺)(DTBMA) 微生物 Qa Qb SI Am Ε. c〇yy 8739 - M9GY 0 60 1.00 (48小時） 40 6 0.50 1/0.2 40 8 0.53 1/0.2 40 10 0.57 1/0.3 40 20 0.73 1/0.5 40 30 0.90 1/0.8 50 3 0.55 1/1.06 50 4 0.57 1/0.08 50 5 0.58 1/0.1 50 6 0.60 1/0.1 50 8 0.63 1/1.2 50 10 0.67 1/0.2 50 20 0.83 1/0.4 60 3 0.65 1/0.05 60 4 0.67 1/0.07 60 5 0.68 1/008 60 6 0.70 1/0.1 60 8 0.73 1/0.1 60 10 0.77 1/0.2 60 20 0.93 1/0.3 70 3 0.75 1/0.04 70 4 0.77 1/0.06 70 5 0.78 1/0.07 70 6 0.80 1/0.09 70 7 0.82 1/0.1 70 8 0.83 1/0.1 70 10 0.87 1/0.1 80 1 0.82 1/0.01 80 2 0.83 1/0.03 39 93646D17 201210506 微生物 Qa Qb SI AJB 80 3 0.85 1/0.04 80 4 0.87 1/0.05 80 5 0.88 1/0.06 80 6 0.90 1/0.08 80 8 0.93 1/0.1 100 0 1.00 微生物 Q. Qb SI Am C. albicans 10231 - PDB 0 2000 1.00 (24小時） 8 40 0.55 1/5 8 50 0.56 1/6 8 60 0.56 1/8 8 80 0.57 1/10 8 100 0.58 1/13 8 200 0.63 1/13 8 300 0.68 1/38 8 400 0.73 1/50 8 500 0.78 1/63 8 600 0.83 1/75 8 800 0.93 1/100 10 1 0.67 1/0.1 10 2 : 0.67 1/0.2 10 3 0.67 1/0.3 10 4 0.67 1/0.4 10 5 0.67 1/0.5 10 6 0.67 1/0.6 10 8 0.67 1/0.8 10 10 0.67 1/1 10 20 0.68 1/2 10 30 0.68 1/3 10 40 0.69 .1/4 10 50 0.69 1/5 10 60 0.70 1/6 10 80 0.71 1/8 10 100 0.72 1/10 10 200 0.77 1/20 10 300 0.82 1/30 10 400 0.87 1/40 10 500 0.92 1/50 15 0 1.00 A. 16404 - PDB 0 1000 1.00 40 93646D17 201210506 微生物 Qa Qb SI A/B (7天） 5 800 0.93 1/160 40 0 1.00 所測之ΒΒΙΤ/二硫基-2,2’-雙(N-甲基苯曱醯胺）之比率範圍自 1/0.02 至 1/500。 41 93646D17 201210506 表11 第一種成分(A)=N-(正-丁基)-1,2-苯并異噻唑啉酮（ΒΒΙΤ) 第二種成分(Β)=乙二胺四乙酸(EDTA) 微生物 Q· Qb SI Am E. coll 8739 - M9GY 0 40000 1.00 (72小時） 40 800 0.42 1/20 40 1000 0.43 1/25 40 1200 0.43 1/30 40 1600 0.44 1/40 40 2000 0.45 1/50 40 4000 0.50 1/100 40 6000 0.55 1/150 40 8000 0.60 1/200 40 10000 0.65 1/250 40 12000 0.70 1/300 40 16000 0.80 1/400 40 20000 0.90 1/500 50 400 0.51 • 1/8 50 600 0.52 1/12 50 800 0.52 1/16 50 1000 0.53 1/20 50 1200 0.53 1/24 50 1600 0.54 1/32 50 2000 0.55 1/40 50 4000 0.60 1/80 50 6000 0.65 1/120 50 8000 0.70 1/160 50 10000 0.75 1/200 50 12000 0.80 1/240 50 16000 0.90 1/320 60 400 0.61 1/7 60 600 0.62 1/10 60 800 0.62 1/13 60 1000 0.63 1/17 60 1200 0.63 1/20 60 1600 0.64 1/27 60 2000 0.65 1/33 42 93646D17 201210506 微生物 Qa Qb SI A/B 60 4000 0.70 1/67 60 6000 0.75 1/100 60 8000 0.80 1/133 60 10000 0.85 1/167 60 12000 0.90 1/200 70 200 0.71 1/3 70 400 0.71 1/6 70 600 0.72 1/9 70 800 0.72 1/11 70 1000 0.73 1/14 70 1200 0.73 1/17. 70 1600 0.74 1/23 70 2000 0.75 1/29 70 4000 0.80 1/57 70 6000 0.85 1/86 70 8000 0.90 1/114 70 10000 0.95 1/143 80 400 0.81 1/5 80 600 0.82 • 1/8 80 800 0.82 1/40 80 1000 0.83 1/13 80 1200 0.83 1/15 80 1600 0.84 1/20 80 2000 0.85 1/25 80 4000 0-90 1/50 80 6000 0.95 1/75 100 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 600 1.00 (24小時） 2.5 60 0.43 1/24 2.5 80 0.47 1/32 2.5 100 0.50 1/40 2.5 120 0.53 1/48 2.5 160 0.60 1/64 2.5 200 0.67 1/80 5 60 0.77 1/12 5 80 0.80 1/16 5 100 0.83 1/20 5 120 0.87 1/24 5 160 0.93 1/32 43 93646D17 201210506 微生物 Qa Qb SI A/B 7.5 0 1.00 A. niger - PDB 0 1600 1.00 (7天） 5 1000 0.75 1/200 5 1200 0.88 1/240 10 400 0.50 1/40 10 600 0.63 1/60 10 800 0.75 1/80 10 1000 0.88 1/100 15 400 0.63 1/27 15 600 0.75 1/40 15 800 0.88 1/53 20 200 0.63 1/10 20 400 0.75 1/20 20 600 0.88 1/30 40 0 1.00 所測之ΒΒΙΤ/乙二胺四乙酸之比率範圍自1/0.4至1Π0000。 44 93646D17 201210506 表12 第一種成分(A)=N-(正-丁基)-1,2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=對羥苯甲酸乙酯微生物 Qa Qb SI A/B E. coJi 8139 - M9GY 0 2000 1.00 (24小時） 60 1000 0.80 1/17 80 800 0.80 1/10 80 1000 0.90 1/13 100 200 0.60 1/2 100 300 0.65 1/3 100 400 0.70 1/4 100 500 0.75 1/5 100 600 0.80 1/6 100 800 0.90 1/8 200 0 1.00 微生物 Q. Qb SI A/B C. albicans 10231 - PDB 0 800 1.00 (24小時） 2.5 80 0.43 1/32 2.5 100 0.46 1/40 2.5 200 0.58 1/80 2.5 300 0.71 1/120 2.5 400 0.83 1/160 2.5 5〇a 0.96 1/200 5 30 0.70 1/6 5 40 0.72 1/8 5 50 0.73 1/10 5 60 0.74 1/12 5 80 0.77 1/16 5 100 0.79 1/20 5 200 0.92 1/40 7.5 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 800 1.00 (7天） 15 600 0.94 1/40 80 0 1.00 所測之ΒΒΠ7對羥苯曱酸乙酯之比率範圍自1/0.2至1/5000。 45 93646D17 201210506 表13 第一種成分(A)=N-(正-丁基)-1，2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=己脒二羥乙基磺酸鹽微生物 Q. Qb SI A/B E. coli 8739 - M9GY 0 100 1.00 (48小時） 40 1 0.57 1/0.03 40 2 0.73 1/0.05 40 3 0.90 1/0.08 50 1 0.67 1/0.02 ， 50 2 0.83 1/0.04 60 1 0.77 1/0.02 60 2 0.93 1/0.03 70 1 0.87 1/0.01 80 1 0.97 1/0.01 6 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 10 1.00 (24小時） 2 4 0.60 1/2 2 5 0.70 1/2.5 2 6 0.80 1/3 4 2 0.60 1/0.5 4 3 0.70 1/0.75 4 4 0.80 1/1 4 5 0.90 1/1.3 6 1 0.70 1/0.2 6 2 0.80 1/0.3 6 3 0.90 1/0.5 8 1 0.90 1/1.1 10 0 1.00 所測之ΒΒΠ7己脒二羥乙基磺酸鹽之比率範圍自1/0.02至 1/500。 46 93646D17 201210506Microorganism Qe Qb SI E. colj 8739 - M9GY 0 200 1.00 (72 hours) 40 8 0.44 1/0.2 40 10 0.45 1/0.3 40 20 0.50 1/0.5 40 30 0.55 1/0.8 40 40 0.60 1/1 40 50 0.65 1/1 40 60 0.70 1/2 40 80 0.80 1/2 40 100 0.90 1/3 50 3 0.52 1/0.06 50 4 0.52 1/0.08 50 5 0.53 1/0.1 50 6 0.53 1/0.1 50 δ 0.54 1/ 0.2 50 10 0.55 1/0.2 50 20 0.60 1/0.4 50 30 0.65 1/0.6 50 40 0.70 1/0.8 50 50 0.75 . 1/1 50 60 0.80 1/1.2 50 80 0.90 1/1.6 60 6 0.63 1/0.1 60 8 0.64 1/0.1 60 10 0.65 1/0.2 60 20 0.70 1/0.3 60 30 0.75 1/0.5 60 40 0.80 1/0.7 60 50 0.85 1/0.8 60 60 0.90 1/1 80 8 0.84 1/0.1 80 10 0.85 1/0.1 80 20 0.90 1/0.3 37 93646D17 201210506 Microorganism Qa Qb SI A/B 100 0 1.00 C. albicans 10231 PDB 0 3000 1.00 (24 hours) 5 600 0.87 1/120 5 800 0.93 1/160 7.5 0 1.00 Microorganism Qe Qb SI A/B A. niger 16404 - PDB 0 1000 1.00 (7 days) 20 80 0.33 1/4 20 100 0.35 1/5 20 200 0.45 1/10 20 300 0.55 1/15 20 400 0.65 1/20 20 500 0.75 1/25 20 600 0.85 1/30 40 100 0.60 1/3 40 200 0.70 1/5 40 300 0.80 1/8 40 400 0.90 1/10 80 0 1.00 The ratio of ΒΒΠ7 DMDM carbendamide measured from 1/0.2 to 1 /5000. 38 93646D17 201210506 Table ίο The first component (A) = N-(n-butyl)-1,2-benzisothiazolinone (ΒΒΙΤ) The second component (Β) = dithio-2,2 '-Bis(甲基-methylbenzamide) (DTBMA) Microorganism Qa Qb SI Am Ε. c〇yy 8739 - M9GY 0 60 1.00 (48 hours) 40 6 0.50 1/0.2 40 8 0.53 1/0.2 40 10 0.57 1/0.3 40 20 0.73 1/0.5 40 30 0.90 1/0.8 50 3 0.55 1/1.06 50 4 0.57 1/0.08 50 5 0.58 1/0.1 50 6 0.60 1/0.1 50 8 0.63 1/1.2 50 10 0.67 1 /0.2 50 20 0.83 1/0.4 60 3 0.65 1/0.05 60 4 0.67 1/0.07 60 5 0.68 1/008 60 6 0.70 1/0.1 60 8 0.73 1/0.1 60 10 0.77 1/0.2 60 20 0.93 1/0.3 70 3 0.75 1/0.04 70 4 0.77 1/0.06 70 5 0.78 1/0.07 70 6 0.80 1/0.09 70 7 0.82 1/0.1 70 8 0.83 1/0.1 70 10 0.87 1/0.1 80 1 0.82 1/0.01 80 2 0.83 1/0.03 39 93646D17 201210506 Microorganism Qa Qb SI AJB 80 3 0.85 1/0.04 80 4 0.87 1/0.05 80 5 0.88 1/0.06 80 6 0.90 1/0.08 80 8 0.93 1/0.1 100 0 1.00 Microbial Q. Qb SI Am C. albicans 10231 - PDB 0 2000 1.00 (24 hours) 8 40 0. 55 1/5 8 50 0.56 1/6 8 60 0.56 1/8 8 80 0.57 1/10 8 100 0.58 1/13 8 200 0.63 1/13 8 300 0.68 1/38 8 400 0.73 1/50 8 500 0.78 1 /63 8 600 0.83 1/75 8 800 0.93 1/100 10 1 0.67 1/0.1 10 2 : 0.67 1/0.2 10 3 0.67 1/0.3 10 4 0.67 1/0.4 10 5 0.67 1/0.5 10 6 0.67 1/ 0.6 10 8 0.67 1/0.8 10 10 0.67 1/1 10 20 0.68 1/2 10 30 0.68 1/3 10 40 0.69 .1/4 10 50 0.69 1/5 10 60 0.70 1/6 10 80 0.71 1/8 10 100 0.72 1/10 10 200 0.77 1/20 10 300 0.82 1/30 10 400 0.87 1/40 10 500 0.92 1/50 15 0 1.00 A. 16404 - PDB 0 1000 1.00 40 93646D17 201210506 Microbial Qa Qb SI A/ B (7 days) 5 800 0.93 1/160 40 0 1.00 The ratio of bismuth/dithio-2,2'-bis(N-methylbenzoguanamine) measured ranges from 1/0.02 to 1/500 . 41 93646D17 201210506 Table 11 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (ΒΒΙΤ) Second component (Β) = ethylenediaminetetraacetic acid (EDTA) Microorganism Q· Qb SI Am E. coll 8739 - M9GY 0 40000 1.00 (72 hours) 40 800 0.42 1/20 40 1000 0.43 1/25 40 1200 0.43 1/30 40 1600 0.44 1/40 40 2000 0.45 1/50 40 4000 0.50 1/100 40 6000 0.55 1/150 40 8000 0.60 1/200 40 10000 0.65 1/250 40 12000 0.70 1/300 40 16000 0.80 1/400 40 20000 0.90 1/500 50 400 0.51 • 1/8 50 600 0.52 1/12 50 800 0.52 1/16 50 1000 0.53 1/20 50 1200 0.53 1/24 50 1600 0.54 1/32 50 2000 0.55 1/40 50 4000 0.60 1/80 50 6000 0.65 1/120 50 8000 0.70 1/160 50 10000 0.75 1/200 50 12000 0.80 1/240 50 16000 0.90 1/320 60 400 0.61 1/7 60 600 0.62 1/10 60 800 0.62 1/13 60 1000 0.63 1/17 60 1200 0.63 1/ 20 60 1600 0.64 1/27 60 2000 0.65 1/33 42 93646D17 201210506 Microorganism Qa Qb SI A/B 60 4000 0.70 1/67 60 6000 0.75 1/100 60 8000 0.80 1/133 60 10000 0.85 1/167 60 12000 0.90 1/200 70 200 0.71 1/3 70 400 0.71 1/6 70 600 0.72 1/9 70 800 0.72 1/11 70 1000 0.73 1/14 70 1200 0.73 1/17. 70 1600 0.74 1/23 70 2000 0.75 1/29 70 4000 0.80 1/57 70 6000 0.85 1/86 70 8000 0.90 1/114 70 10000 0.95 1/143 80 400 0.81 1/5 80 600 0.82 • 1/8 80 800 0.82 1/40 80 1000 0.83 1/13 80 1200 0.83 1/15 80 1600 0.84 1/20 80 2000 0.85 1/25 80 4000 0-90 1/50 80 6000 0.95 1/75 100 0 1.00 Microbial Qa Qb SI A/B C. albicans 10231 - PDB 0 600 1.00 (24 hours) 2.5 60 0.43 1/24 2.5 80 0.47 1/32 2.5 100 0.50 1/40 2.5 120 0.53 1/48 2.5 160 0.60 1/64 2.5 200 0.67 1/80 5 60 0.77 1/ 12 5 80 0.80 1/16 5 100 0.83 1/20 5 120 0.87 1/24 5 160 0.93 1/32 43 93646D17 201210506 Microorganism Qa Qb SI A/B 7.5 0 1.00 A. niger - PDB 0 1600 1.00 (7 days) 5 1000 0.75 1/200 5 1200 0.88 1/240 10 400 0.50 1/40 10 600 0.63 1/60 10 800 0.75 1/80 10 1000 0.88 1/100 15 400 0.63 1/27 15 600 0.75 1/40 15 800 0.88 1/53 20 200 0.63 1/10 20 400 0.75 1/20 20 600 0.88 1/30 40 0 1.00 The ratio of ruthenium/ethylenediaminetetraacetic acid measured ranges from 1/0.4 to 1Π0000. 44 93646D17 201210506 Table 12 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = ethyl paraben Qa Qb SI A/B E. coJi 8139 - M9GY 0 2000 1.00 (24 hours) 60 1000 0.80 1/17 80 800 0.80 1/10 80 1000 0.90 1/13 100 200 0.60 1/2 100 300 0.65 1/3 100 400 0.70 1/4 100 500 0.75 1/5 100 600 0.80 1/6 100 800 0.90 1/8 200 0 1.00 Microbial Q. Qb SI A/B C. albicans 10231 - PDB 0 800 1.00 (24 hours) 2.5 80 0.43 1/32 2.5 100 0.46 1/40 2.5 200 0.58 1/80 2.5 300 0.71 1/120 2.5 400 0.83 1/160 2.5 5〇a 0.96 1/200 5 30 0.70 1/6 5 40 0.72 1/8 5 50 0.73 1/10 5 60 0.74 1/12 5 80 0.77 1/16 5 100 0.79 1/20 5 200 0.92 1/40 7.5 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 800 1.00 (7 days) 15 600 0.94 1/40 80 0 1.00 The ratio of ΒΒΠ7-p-hydroxybenzoic acid ethyl ester measured ranges from 1/0.2 to 1/5000. 45 93646D17 201210506 Table 13 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = hexamethylene dihydroxyethyl sulfonate Acid salt microorganism Q. Qb SI A/B E. coli 8739 - M9GY 0 100 1.00 (48 hours) 40 1 0.57 1/0.03 40 2 0.73 1/0.05 40 3 0.90 1/0.08 50 1 0.67 1/0.02 , 50 2 0.83 1/0.04 60 1 0.77 1/0.02 60 2 0.93 1/0.03 70 1 0.87 1/0.01 80 1 0.97 1/0.01 6 0 1.00 Microbial Qa Qb SI A/B C. albicans 10231 - PDB 0 10 1.00 (24 hours) ) 2 4 0.60 1/2 2 5 0.70 1/2.5 2 6 0.80 1/3 4 2 0.60 1/0.5 4 3 0.70 1/0.75 4 4 0.80 1/1 4 5 0.90 1/1.3 6 1 0.70 1/0.2 6 2 0.80 1/0.3 6 3 0.90 1/0.5 8 1 0.90 1/1.1 10 0 1.00 The ratio of ΒΒΠ7 hexamethylene disulfonate measured ranges from 1/0.02 to 1/500. 46 93646D17 201210506

表14第一種成分(A)=N-(正-丁基)-1，2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=雙辛氫啶微生物 Qb si A/B E. coll 8139 - M9GY 〇 (72小時） 40 40 40 40 40 40 50 50 50 50 50 60 60 60 60 60 60 60 60 60 70 70 70 70 70 70 70 70 80 80 80 80 00000 0 0 0 0 0004 5 6 8 0 0 0 00 01234 5 6 1 2 345 1 2 3 4 5 5 6 8 10203040 504 6 8 1.00 0.33 1/0.3 0.45 1/1.5 0.58 1/0.8 0.70 1/1 0.83 1/1.3 0.95 1/1.5 0.38 1/0.2 0.50 1/0.4 0.63 1/0.6 0.75 1/0.8 0.88 1/1 0.35 1/0.07 0.36 1/0.08 ' 0.38 1/0.1 0.40 1/0.1 0.43 1/0.2 0.55 1/0.3 0.68 1/0.5 0.80 1/0.7 0.93 1/0.8 0.41 1/0.07 0.43 1/0.09 0.45 1/0.1 0.48 1/0.1 0.60 1/0.3 0.73 1/0.4 0.85 1/0.6 0.98 1/0.7 0.45 1/0.05 0.46 1/0.06 0.48 1/0.08 0.50 1/0.1 93646D17 201210506 微生物 Q·Table 14 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = dioctylhydrogenidine microorganism Qb si A/B E. coll 8139 - M9GY 〇 (72 hours) 40 40 40 40 40 40 50 50 50 50 50 60 60 60 60 60 60 60 60 60 70 70 70 70 70 70 70 70 80 80 80 80 00000 0 0 0 0 0004 5 6 8 0 0 0 00 01234 5 6 1 2 345 1 2 3 4 5 5 6 8 10203040 504 6 8 1.00 0.33 1/0.3 0.45 1/1.5 0.58 1/0.8 0.70 1/1 0.83 1/1.3 0.95 1/1.5 0.38 1/0.2 0.50 1/0.4 0.63 1/0.6 0.75 1/0.8 0.88 1/1 0.35 1/0.07 0.36 1/0.08 ' 0.38 1/0.1 0.40 1/0.1 0.43 1/0.2 0.55 1/0.3 0.68 1/0.5 0.80 1 /0.7 0.93 1/0.8 0.41 1/0.07 0.43 1/0.09 0.45 1/0.1 0.48 1/0.1 0.60 1/0.3 0.73 1/0.4 0.85 1/0.6 0.98 1/0.7 0.45 1/0.05 0.46 1/0.06 0.48 1/0.08 0.50 1/0.1 93646D17 201210506 Microorganism Q·

Qb si oo o o o o oo o oooooo 88 8 8 ο o oo o 000008 1 111 1 1 1 1 11 微生物 C. albicans 10231 - PDB (72小時） 02 2224444666 6888888810101010Qb si oo o o o o oo o oooooo 88 8 8 ο o oo o 000008 1 111 1 1 1 1 11 Microorganisms C. albicans 10231 - PDB (72 hours) 02 2224444666 6888888810101010

Qb 10 0.53 1/0.1 20 0.65 1/0.3 30 0.78 1/0.4 40 0.90 1/0.5 1 0.51 1/0.01 2 0.53 1/0.02 3 0.54 1/0.03 4 0.55 1/0.04 5 0.56 1/0.05 6 0.58 1/0.06 8 0.60 1/0.08 10 0.63 1/0.1 20 0.75 1/0.2 30 0.88 1/0.3 0 1.00 SI A/B 100 1.00 40 0.53 1/20 50 0.63 1/25 60 0.73 1/30 80 0.93 1/40 30 0.57 1/8 40 0.67 1/10 50 0.77 1/13 60 0.87 1/15 20 0.60 1/3 30 0.70 1/5 40 0.80 1/7 50 0.90 1/9 5 0.58 1/0.6 6 0.59 1/0.8 8 0.61 1/1 10 0.63 1/1 20 0.73 1/3 30 0.83 1/4 40 0.93 1/5 1 0.68 1/0.1 2 0.69 1/0.2 3 0.70 1/0.3 4 0.71 1/0.4 48 93646D17 201210506 微生物 Qb SI. A/B 10 5 0.72 1/0.5 10 6 0.73 1/0.6 10 8 0.75 1/0.8 10 10 0.77 1/1 10 20 0.87 1/2 10 30 0.97 1/3 15 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 · PDB 0 100 1.00 (4天） 5 50 0.75 1/10 5 60 0.85 1/12 10 40 0.90 1/4 20 0 1.00 所測之BBIT/雙辛氫啶之比率範圍自1/0.02至1/500。 49 93646D17 201210506 表15 第一種成分(A)=N-(正-丁基)-1，2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=己二醇微生物 Q. Qb SI Am E. coli 8139 - M9GY 0 20000 1.00 (24小時） 40 20000 1.40 1/500 50 20000 1.50 1/400 60 20000 1.60 1/333 80 20000 1.80 1/250 100 0 1.00 微生物 Q. Qb SI AiB C. albicans 10231 - PDB 0 20000 1.00 (24小時） 2 20000 1.20 1/10000 4 20000 1.40 1/5000 6 20000 1.60 1/3333 8 20000 1.80 1/2800 10 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 20000 1.00 (3天） 5 20000 1.33 1/4000 10 20000 1.67 1/2000 15 0 1.00 所測之BBIT/己二醇之比率範圍自1/0.2至1/5000。BBIT 和己二醇間無觀察到協同作用。 50 93646D17 201210506 表16 第一種成分(A)=N-(正-丁基)-1，2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=對羥苯曱酸曱酯微生物 Q. Qb SI Am E. co7/8739 - M9GY 0 2000 1.00 (72小時） 40 400 0.60 1/10 40 500 0.65 1/13 40 600 0.70 1/15 40 800 0.80 1/20 40 1000 0.90 1/25 50 100 0.55 1/2 50 200 0.60 1/4 50 300 0.65 1/6 50 400 0.70 1/8 50 500 0.75 1/10 50 600 0.80 1/12 50 800 0.90 1/16 60 30 0.62 1/0.5 · 60 40 0.62 1/0.7 60 50 0.63 1/0.8 60 60 0.63 1/1 60 80 0.64 1/1 60 100 0.65 1/2 60 200 0.70 1/3 60 300 0.75 1/5 60 400 0.80 1/7 60 500 0.85 1/8 60 600 0.90 1/10 70 300 0.85 1/4 70 400 0.90 1/6 70 500 0.95 1/7 80 300 0.95 1/4 100 0 1.00 微生物 Qa Qb SI Am C. albicans 10231 - PDB 0 1000 1.00 (24小時） 2.5 100 0.43 1/40 2.5 200 0.53 1/80 51 93646D17 201210506 微生物 Q- Qb SI A/B 2.5 300 0.63 1/120 2.5 400 0.73 1/160 2.5 500 0.83 1/200 2.5 600 0.93 1/240 5 40 0.71 1/8 5 50 0.72 1/10 5 60 0.73 1/12 5 80 0.75 1/16 5 100 0.77 1/20 5 200 0.87 1/40 5 300 0.97 1/60 ‘ 7.5 0 1.00 微生物 Q. Qb SI A/B A. niger 16404 - PDB 0 1000 1.00 (3天） 5 600 0.73 1/120 5 800 0.93 1/160 40 0 1.00 所測之ΒΒΙΤ/對羥苯曱酸曱酯之比率範圍自1/0.2至 1/5000。 52 93646D17 201210506 表17 第一種成分(A)=N-(正-丁基)-1，2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=戊二醇微生物 Qa Qb SI A/B E. <707/8739 - M9GY 0 20000 1.00 (24小時） 40 20000 1.40 1/500 60 20000 1.60 1/333 80 20000 1.80 1/250 100 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 20000 1.00 (24小時） 2 20000 1.02 1/10000 4 20000 1.04 1/5000 8 20000 1.08 10 20000 1.10 15 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 20000 1.00 (4天） 5 20000 1.05 10 20000 1.10 15 20000 1.15 20 0 1.00 所測之BBIT/戊二醇之比率範圍自1/0.2至1/5000。BBIT 和戊二醇間無觀察到協同作用。 53 93646D17 201210506 表18 第一種成分(A)=N-(正-丁基)-1,2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=苯氧基乙醇微生物 Q. Qb SI AJB E. coJi 8739 * M9GY 0 5000 1.00 (72小時） 50 800 0.66 1/16 50 1000 0.70 1/20 50 2000 0.90 1/40 60 60 0.61 1/1 60 80 0.62 1/1 60 100 0.62 1/2 . 60 200 0.64 1/3 60 300 0.66 1/5 60 400 0.68 1/7 60 500 0.70 1/8 60 600 0.72 1/10 60 800 0.76 1/13 60 1000 0.80 1/17 70 1000 0.90 1/14 100 0 1.00 微生物 Q- Qb SI Am C. albicans 10231 - PDB 0 4000 1.00 (24小時） 2.5 1000 0.58 1/400 2.5 2000 0.83 1/800 5 500 0.79 1/100 5 600 0.82 1/120 5 800 0.87 1/160 5 1000 0.92 1/200 7.5 0 1.00 微生物 Qa Qb SI AfB A. niger 16404 - PDB 0 4000 1.00 (7天） 5 3000 0.88 1/600 40 0 1.00 所測之BBIT/苯氧基乙醇之比率範圍自1/0.2至1/5000。 54 93646D17 201210506 表19 第一種成分(A)=N-(正-丁基)-1,2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=亞麻油醯胺丙基丙二醇-氯化二甲基銨磷酸酯（磷脂EFA) 微生物 Qa Qb SI A/B E. coJi 8139 - M9GY 0 600 1.00 (48小時） 40 400 0.87 1/10 50 400 0.92 1/8 200 0 1.00 微生物 Q. Qb SI A/B C. albicans 10231 * PDB 0 2000 1.00 (24小時） 2 800 0.60 1/400 2 1000 0.70 1/500 4 600 0.70 1/150 4 800 0.80 1/200 4 1000 0.90 1/250 6 500 0.85 1/83 6 600 0.90 1/100 10 0 1.00 微生物 Q. Qb SI A/B A. niger 16404 · PDB 0 2000 1.00 (4天） 5 600 0.55 1/120 5 800 0.65 1/160 5 1000 0.75 1/200 10 600 0.80 1/60 10 800 0.90 1/80 20 0 1.00 所測之BBIT/亞麻油醯胺丙基丙二醇-氯化二曱基銨磷酸酯之比率範圍自1/0.02至1/500。 55 93646D17 201210506 表20 第一種成分(A)=N-(正-丁基)-1,2-苯并異噻唑啉酮(丑；8汀）第二種成分（B)=椰子油醯胺丙基丙二醇-氯化二曱基銨磷酸酯（磷脂PTC) 微生物 Q. Qb SI AfB E. coli 8139 - M9GY 0 80 1.00 (72小時） 40 20 0.45 1/0.5 40 30 0.58 1/0.75 40 40 0.70 1/1 40 50 0.83 1/0.3 40 60 0.95 1/2 50 20 0.50 1/0.4 50 30 0.63 1/0.6 50 40 0.75 1/0.8 50 50 0.88 1/1 60 8 0.40 1/0.1 60 10 0.43 1/0.2 60 20 0.55 1/0.3 60 30 0.68 1/0.5 60 40 0.80 1/0.7 60 50 0.93 1/0.8 70 10 0.48 1/0.1 70 20 0.60 1/0.3 70 30 0.73 1/0.4 70 40 0.85 1/0.6 70 50 0.98 1/0.7 80 10 0.53 1/0.1 80 20 0.65 1/0.3 80 30 0.78 1/0.4 80 40 0.90 1/0.5 100 3 0.54 1/0.3 100 4 0.55 1/0.4 100 5 0.56 1/0.5 100 6 0.58 1/0.6 100 8 0.60 1/0.8 100 10 0.63 1/0.1 56 93646D17 201210506Qb 10 0.53 1/0.1 20 0.65 1/0.3 30 0.78 1/0.4 40 0.90 1/0.5 1 0.51 1/0.01 2 0.53 1/0.02 3 0.54 1/0.03 4 0.55 1/0.04 5 0.56 1/0.05 6 0.58 1/ 0.06 8 0.60 1/0.08 10 0.63 1/0.1 20 0.75 1/0.2 30 0.88 1/0.3 0 1.00 SI A/B 100 1.00 40 0.53 1/20 50 0.63 1/25 60 0.73 1/30 80 0.93 1/40 30 0.57 1/8 40 0.67 1/10 50 0.77 1/13 60 0.87 1/15 20 0.60 1/3 30 0.70 1/5 40 0.80 1/7 50 0.90 1/9 5 0.58 1/0.6 6 0.59 1/0.8 8 0.61 1/1 10 0.63 1/1 20 0.73 1/3 30 0.83 1/4 40 0.93 1/5 1 0.68 1/0.1 2 0.69 1/0.2 3 0.70 1/0.3 4 0.71 1/0.4 48 93646D17 201210506 Microbial Qb SI A/B 10 5 0.72 1/0.5 10 6 0.73 1/0.6 10 8 0.75 1/0.8 10 10 0.77 1/1 10 20 0.87 1/2 10 30 0.97 1/3 15 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 · PDB 0 100 1.00 (4 days) 5 50 0.75 1/10 5 60 0.85 1/12 10 40 0.90 1/4 20 0 1.00 The ratio of BBIT/double octane hydrochloride measured ranges from 1/0.02 To 1/500. 49 93646D17 201210506 Table 15 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = hexanediol microorganism Q. Qb SI Am E. coli 8139 - M9GY 0 20000 1.00 (24 hours) 40 20000 1.40 1/500 50 20000 1.50 1/400 60 20000 1.60 1/333 80 20000 1.80 1/250 100 0 1.00 Microbial Q. Qb SI AiB C. Albicans 10231 - PDB 0 20000 1.00 (24 hours) 2 20000 1.20 1/10000 4 20000 1.40 1/5000 6 20000 1.60 1/3333 8 20000 1.80 1/2800 10 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 20000 1.00 (3 days) 5 20000 1.33 1/4000 10 20000 1.67 1/2000 15 0 1.00 The ratio of BBIT/hexanediol measured ranges from 1/0.2 to 1/5000. No synergy was observed between BBIT and hexanediol. 50 93646D17 201210506 Table 16 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = oxime hydroxybenzoate Microorganism Q. Qb SI Am E. co7/8739 - M9GY 0 2000 1.00 (72 hours) 40 400 0.60 1/10 40 500 0.65 1/13 40 600 0.70 1/15 40 800 0.80 1/20 40 1000 0.90 1/25 50 100 0.55 1/2 50 200 0.60 1/4 50 300 0.65 1/6 50 400 0.70 1/8 50 500 0.75 1/10 50 600 0.80 1/12 50 800 0.90 1/16 60 30 0.62 1/0.5 · 60 40 0.62 1/0.7 60 50 0.63 1/0.8 60 60 0.63 1/1 60 80 0.64 1/1 60 100 0.65 1/2 60 200 0.70 1/3 60 300 0.75 1/5 60 400 0.80 1/7 60 500 0.85 1/8 60 600 0.90 1/10 70 300 0.85 1/4 70 400 0.90 1/6 70 500 0.95 1/7 80 300 0.95 1/4 100 0 1.00 Microbial Qa Qb SI Am C. albicans 10231 - PDB 0 1000 1.00 (24 hours) 2.5 100 0.43 1/40 2.5 200 0.53 1/80 51 93646D17 201210506 Microorganism Q- Qb SI A/B 2.5 300 0.63 1/120 2.5 400 0.73 1/160 2.5 500 0.83 1/200 2.5 600 0.93 1/ 240 5 40 0.71 1/8 5 50 0.72 1/10 5 60 0.73 1/12 5 80 0.75 1/16 5 100 0.77 1/20 5 200 0.87 1/40 5 300 0.97 1/60 ' 7.5 0 1.00 Microbial Q. Qb SI A/B A. niger 16404 - PDB 0 1000 1.00 (3 days) 5 600 0.73 1/120 5 800 0.93 1/160 40 0 1.00 The ratio of hydrazine/p-hydroxybenzoate is from 1/0.2 to 1/5000. 52 93646D17 201210506 Table 17 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = pentanediol microorganism Qa Qb SI A/B E. <707/8739 - M9GY 0 20000 1.00 (24 hours) 40 20000 1.40 1/500 60 20000 1.60 1/333 80 20000 1.80 1/250 100 0 1.00 Microbial Qa Qb SI A/B C. albicans 10231 - PDB 0 20000 1.00 (24 hours) 2 20000 1.02 1/10000 4 20000 1.04 1/5000 8 20000 1.08 10 20000 1.10 15 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 20000 1.00 (4 days) 5 20000 1.05 10 20000 1.10 15 20000 1.15 20 0 1.00 The ratio of BBIT/pentanediol measured ranges from 1/0.2 to 1/5000. No synergistic effect was observed between BBIT and pentanediol. 53 93646D17 201210506 Table 18 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = phenoxyethanol microorganism Q. Qb SI AJB E. coJi 8739 * M9GY 0 5000 1.00 (72 hours) 50 800 0.66 1/16 50 1000 0.70 1/20 50 2000 0.90 1/40 60 60 0.61 1/1 60 80 0.62 1/1 60 100 0.62 1 /2 . 60 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 100 0 1.00 Microbial Q- Qb SI Am C. albicans 10231 - PDB 0 4000 1.00 (24 hours) 2.5 1000 0.58 1/400 2.5 2000 0.83 1/800 5 500 0.79 1/100 5 600 0.82 1/120 5 800 0.87 1/160 5 1000 0.92 1/200 7.5 0 1.00 Microbial Qa Qb SI AfB A. niger 16404 - PDB 0 4000 1.00 (7 days) 5 3000 0.88 1/600 40 0 1.00 BBIT/phenoxyethanol ratio measured The range is from 1/0.2 to 1/5000. 54 93646D17 201210506 Table 19 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = linseed oil allylpropyl propylene glycol - dimethylammonium chloride phosphate (phospholipid EFA) microorganism Qa Qb SI A/B E. coJi 8139 - M9GY 0 600 1.00 (48 hours) 40 400 0.87 1/10 50 400 0.92 1/8 200 0 1.00 Microbial Q Qb SI A/B C. albicans 10231 * PDB 0 2000 1.00 (24 hours) 2 800 0.60 1/400 2 1000 0.70 1/500 4 600 0.70 1/150 4 800 0.80 1/200 4 1000 0.90 1/250 6 500 0.85 1/83 6 600 0.90 1/100 10 0 1.00 Microbial Q. Qb SI A/B A. niger 16404 · PDB 0 2000 1.00 (4 days) 5 600 0.55 1/120 5 800 0.65 1/160 5 1000 0.75 1/200 10 600 0.80 1/60 10 800 0.90 1/80 20 0 1.00 The ratio of BBIT/linolenic acid allylpropyl propylene glycol-dianonyl ammonium phosphate measured ranges from 1/0.02 to 1/500. . 55 93646D17 201210506 Table 20 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (ugly; 8 ting) Second component (B) = coconut oil decylamine Propyl propylene glycol-dianonyl ammonium phosphate (phospholipid PTC) microorganism Q. Qb SI AfB E. coli 8139 - M9GY 0 80 1.00 (72 hours) 40 20 0.45 1/0.5 40 30 0.58 1/0.75 40 40 0.70 1/1 40 50 0.83 1/0.3 40 60 0.95 1/2 50 20 0.50 1/0.4 50 30 0.63 1/0.6 50 40 0.75 1/0.8 50 50 0.88 1/1 60 8 0.40 1/0.1 60 10 0.43 1/ 0.2 60 20 0.55 1/0.3 60 30 0.68 1/0.5 60 40 0.80 1/0.7 60 50 0.93 1/0.8 70 10 0.48 1/0.1 70 20 0.60 1/0.3 70 30 0.73 1/0.4 70 40 0.85 1/0.6 70 50 0.98 1/0.7 80 10 0.53 1/0.1 80 20 0.65 1/0.3 80 30 0.78 1/0.4 80 40 0.90 1/0.5 100 3 0.54 1/0.3 100 4 0.55 1/0.4 100 5 0.56 1/0.5 100 6 0.58 1/0.6 100 8 0.60 1/0.8 100 10 0.63 1/0.1 56 93646D17 201210506

微生物 Qa Qb SI 100 20 0.75 1/0.2 100 30 0.88 1/0.3 200 0 1.00 微生物 Q- Qb SI A/B C. albicans 10231 - PDB 0 1000 1.00 (24小時） 2 600 0.73 1/300 2 800 0.93 1/400 4 400 0.67 1/400 4 500 0.77 1/125 4 600 0.87 1/150 6 300 0.70 1/50 6 400 0.80 1/67 6 500 0.90 1/83 8 4 0.54 1/0.5 8 5 . 0.54 1/0.6 8 6 0.54 1/0.8 8 8 0.54 1/1 8 10 0.54 1/1 8 20 0.55 1/6 8 30 0.56 1/4 8 40 0.57 1/5 8 50 0.58 1/6 8 60 0.59 1/8 8 80 0.61 1/10 8 100 0.63 1/13 8 200 0.73 1/25 8 300 0.83 1/38 8 400 0.93 1/50 10 3 0.67 1/0.3 10 4 0.67 1/0.4 10 5 0.67 1/0.5 10 6 0.67 1/0.6 10 8 0.67 1/0.8 10 10 0.68 1/1 10 20 0.69 1/2 10 30 0.70 1/3 57 93646D17 201210506 微生物 Q» Qb SI A/B 10 40 0.71 1/4 10 50 0.72 1/5 10 60 0.73 1/6 10 80 0.75 1/8 10 100 0.77 1/10 10 200 0.87 1/20 10 300 0.97 1/30 15 0 1.00 微生物 Qa Qb SI A/B A. ni^er 16404 - PDB 0 1000 1.00 … . (3天） 5 500 0.83 1/100 5 600 0.93 1/120 10 200 0.87 1/20 10 300 0.97 1/30 15 0 1.00 所測之BBIT/椰子油醯胺丙基丙二醇-氣化二曱基銨磷酸酯之比率範圍自1/0.02至1/500。 58 93646D17 201210506 表21 第一種成分(A)=N-(正-丁基)-l，2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=山梨酸鉀微生物 Qa Qb SI A/B E. coJi 8139 · M9GY 0 20000 1.00 (24小時） 20 20000 1.10 1/1000 40 20000 1.20 1/500 60 20000 1.30 1/333 80 20000 1.40 1/250 100 20000 1.50 1/200 200 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 PDB .0 400 1.00 (24小時） 2.5 100 0.58 1/40 2.5 200 0.83 1/80 5 30 0.74 1/6 5 60 0.82 1/12 5 80 0.87 1/16 5 100 0.92 1/20 7.5 0 1.00 v 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 8000 1.00 (3天) 5 6000 0.88 1/1200 10 4000 0.75 1/400 10 5000 0.88 1/500 15 4000 0.88 1/267 20 600 0.58 1/30 20 800 0.60 1/40 20 1000 0.63 1/50 20 2000 0.75 1/100 20 3000 0.88 1/150 40 0 1.00 所測之BBIT/山梨酸鉀之比率範圍自1/0.2至1/5000。 59 93646D17 201210506 表22 第一種成分(A)=N-(正-丁基)-l，2-笨并異噻唑啉酮(BBIT) 第二種成分(B)=對羥苯曱酸丙酯微生物 Qa Qb SI AfB E. coli B739 - M9GY 0 2000 1.00 (24小時） 20 2000 1,10 1/100 40 2000 1.20 1/50 60 2000 1.30 1/33 80 2000 1.40 1/25 100 2000 1.50 1/25 200 0 1,00 微生物 Qa Qb SI AfB C. albicans 10231 - PDB 0 2000 1.00 (24小時） 2.5 30 0.35 1/12 2.5 40 0.35 1/16 2.5 50 0.36 1/20 2.5 60 0.36 1/24 2.5 80 0.37 1/32 2.5 100 0.38 1/40 2.5 200 0.43 1/80 2.5 300 0.48 1/120 2.5 400 0.53 1/160 2.5 500 0.58 1/200 2.5 600 0.63 1/240 2.5 800 0.73 1/320 2.5 1000 0.83 1/400 5 20 0.68 1/4 5 30 0.68 1/6 5 40 0.69 1/8 5 50 0.69 1/10 5 60 0.70 1/12 5 80 0.71 1/16 5 100 0.72 1/20 5 200 0.77 1/40 5 300 0.82 1/60 5 400 0.87 1/80 5 500 0.92 1/100 60 93646D17 201210506 微生物 Qa Qb SI A/B 5 600 0.97 1/120 7.5 0 1.00 微生物 Q. Qb SI A/B A. niger 16404 · PDB 0 2000 1.00 (7天） 10 1000 0.63 1/100 20 800 0.65 1/40 20 1000 0.75 1/80 40 800 0.90 1/20 80 0 1.00 所測之ΒΒΙΤ/對羥苯曱酸丙酯之比率範圍自1/0.2至 1/5000。 61 93646D17 201210506 表23 第一種成分(A)=N-(正-丁基)-1，2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=Quaternium-15(活化-1-(3-氣烯丙基)-3,5,7-三氮雜-1-氮鏽金剛烷氣化物）微生物 Qa Qb SI A/B E. coli 8139 · M9GY 0 200 1.00 (72小時） 40 50 0.43 1/1 40 60 0.43 1/1 40 80 0.44 1/2 40 100 0.45 1/3 40 200 0.50 1/5 40 300 0.55 1/8 40 400 0.60 1/10 40 500 0.65 1/13 40 600 0.70 1/15 40 800 0.80 1/20 200 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 600 1.00 (24小時） 5 50 0.75 1/10 5 60 0.77 1/12 5 80 0.80 1/16 5 100 0.83 1/20 7.5 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 3000 1.00 (4天〉 10 1000 0.58 1/100 10 2000 0.92 1/200 20 1000 0.83 1/50 40 0 1.00 所測之ΒΒΙΤ/1-(3-氯烯丙基）-3,5,7-三氮雜-1-氮鏽金剛烷氯化物之比率範圍自1/0.02至1/5000。 62 93646D17 201210506 表24 第一種成分(A)=N-(正-丁基)-1,2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=去氫乙酸，鈉鹽（SDHA) 微生物 Qa Qb SI A/B E. coJi 8739 - M9GY 0 20000 1.00 (24小時） 20 20000 1.10 1/1000 40 20000 1.20 1/500 60 20000 1.30 1/333 80 20000 1.40 1/250 100 20000 1.50 1/200 200 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 40 1.00 (24小時） 4 10 0.75 1/3 6 4 0.85 1/0.7 6 5 0.88 1/0.8 6 6 0.90 1/1 6 8 0.95 1/1 8 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 200 1.00 (7天） 20 80 0.65 1/4 20 100 0.75 1/5 40 50 0.75 1/3 40 60 0.80 1/2 40 80 0.90 1/2 80 0 1.00 所測之BBIT/去氫乙酸（鈉鹽）之比率範圍自1/0.02至 1/5000。 63 93646D17 201210506 表25 第一種成分(A)=N-(正-丁基)-1，2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=苯曱酸鈉微生物 Qa Qb SI A/B E. coZi'8739 - M9GY 0 20000 1.00 (24小時） 20 20000 1.10 1/1000 40 20000 1.20 1/500 60 20000 1.30 1/333 80 20000 1.40 1/250 100 20000 1.50 1/200 200 0 1.00 微生物 Qa Q«. SI A/B C. albicans 10231 - PDB 0 2000 1.00 (48小時） 2.5 400 0.53 1/160 2.5 500 0.58 1/200 2.5 600 0.63 1/240 2.5 800 0.73 1/320 2.5 1000 0.83 ι/4〇σ 5 300 0.82 1/60 5 400 0.87 1/80 5 500 0.92 1/100 5 600 0.97 1/120 7.5 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 10000 1.00 (7天） 5 8000 0.86 1/1600 10 3000 0.43 1/300 10 4000 0.53 1/400 10 5000 0.63 1/500 10 6000 0.73 1/600 10 8000 0.93 1/800 15 3000 0.49 1/200 15 4000 0.59 1/267 15 5000 0.69 1/333 15 6000 0.79 1/400 15 7000 0.89 1/467 15 8000 0.99 1/533 64 93646D17 201210506Microorganism Qa Qb SI 100 20 0.75 1/0.2 100 30 0.88 1/0.3 200 0 1.00 Microbial Q- Qb SI A/B C. albicans 10231 - PDB 0 1000 1.00 (24 hours) 2 600 0.73 1/300 2 800 0.93 1 /400 4 400 0.67 1/400 4 500 0.77 1/125 4 600 0.87 1/150 6 300 0.70 1/50 6 400 0.80 1/67 6 500 0.90 1/83 8 4 0.54 1/0.5 8 5 . 0.54 1/ 0.6 8 6 0.54 1/0.8 8 8 0.54 1/1 8 10 0.54 1/1 8 20 0.55 1/6 8 30 0.56 1/4 8 40 0.57 1/5 8 50 0.58 1/6 8 60 0.59 1/8 8 80 0.61 1/10 8 100 0.63 1/13 8 200 0.73 1/25 8 300 0.83 1/38 8 400 0.93 1/50 10 3 0.67 1/0.3 10 4 0.67 1/0.4 10 5 0.67 1/0.5 10 6 0.67 1/0.6 10 8 0.67 1/0.8 10 10 0.68 1/1 10 20 0.69 1/2 10 30 0.70 1/3 57 93646D17 201210506 Microorganism Q» Qb SI A/B 10 40 0.71 1/4 10 50 0.72 1/5 10 60 0.73 1/6 10 80 0.75 1/8 10 100 0.77 1/10 10 200 0.87 1/20 10 300 0.97 1/30 15 0 1.00 Microbial Qa Qb SI A/B A. ni^er 16404 - PDB 0 1000 1.00 ... . (3 days) 5 500 0.83 1/100 5 600 0.93 1/120 10 200 0.87 1/20 10 300 0.97 1/30 15 0 1.00 The ratio of BBIT/coconut oil guanidinium propyl propylene glycol-vaporized decyl ammonium phosphate was measured from 1/0.02 to 1/500. 58 93646D17 201210506 Table 21 First component (A) = N-(n-butyl)-l,2-benzisothiazolinone (BBIT) Second component (B) = potassium sorbate microorganism Qa Qb SI A/B E. coJi 8139 · M9GY 0 20000 1.00 (24 hours) 20 20000 1.10 1/1000 40 20000 1.20 1/500 60 20000 1.30 1/333 80 20000 1.40 1/250 100 20000 1.50 1/200 200 0 1.00 Microorganisms Qa Qb SI A/B C. albicans 10231 PDB .0 400 1.00 (24 hours) 2.5 100 0.58 1/40 2.5 200 0.83 1/80 5 30 0.74 1/6 5 60 0.82 1/12 5 80 0.87 1/16 5 100 0.92 1/20 7.5 0 1.00 v Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 8000 1.00 (3 days) 5 6000 0.88 1/1200 10 4000 0.75 1/400 10 5000 0.88 1/500 15 4000 0.88 1/267 20 600 0.58 1/30 20 800 0.60 1/40 20 1000 0.63 1/50 20 2000 0.75 1/100 20 3000 0.88 1/150 40 0 1.00 The ratio of BBIT/potassium sorbate measured ranges from 1/1 0.2 to 1/5000. 59 93646D17 201210506 Table 22 First component (A) = N-(n-butyl)-l, 2- benzoisothiazolinone (BBIT) Second component (B) = propyl paraben Microorganism Qa Qb SI AfB E. coli B739 - M9GY 0 2000 1.00 (24 hours) 20 2000 1,10 1/100 40 2000 1.20 1/50 60 2000 1.30 1/33 80 2000 1.40 1/25 100 2000 1.50 1/25 200 0 1,00 Microbial Qa Qb SI AfB C. albicans 10231 - PDB 0 2000 1.00 (24 hours) 2.5 30 0.35 1/12 2.5 40 0.35 1/16 2.5 50 0.36 1/20 2.5 60 0.36 1/24 2.5 80 0.37 1/32 2.5 100 0.38 1/40 2.5 200 0.43 1/80 2.5 300 0.48 1/120 2.5 400 0.53 1/160 2.5 500 0.58 1/200 2.5 600 0.63 1/240 2.5 800 0.73 1/320 2.5 1000 0.83 1/ 400 5 20 0.68 1/4 5 30 0.68 1/6 5 40 0.69 1/8 5 50 0.69 1/10 5 60 0.70 1/12 5 80 0.71 1/16 5 100 0.72 1/20 5 200 0.77 1/40 5 300 0.82 1/60 5 400 0.87 1/80 5 500 0.92 1/100 60 93646D17 201210506 Microorganism Qa Qb SI A/B 5 600 0.97 1/120 7.5 0 1.00 Microbial Q. Qb SI A/B A. niger 16404 · PDB 0 2000 1.00 (7 10 1000 0.63 1/100 20 800 0.65 1/40 20 1000 0.75 1/80 40 800 0.90 1/20 80 0 1.00 The ratio of ΒΒΙΤ/p-hydroxybenzoic acid phenol measured ranges from 1/0.2 to 1/1 5000. 61 93646D17 201210506 Table 23 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = Quaternium-15 (activation-1 -(3-Allyl)-3,5,7-triaza-1-nitrogenadamantane gasification) Microorganism Qa Qb SI A/B E. coli 8139 · M9GY 0 200 1.00 (72 hours) 40 50 0.43 1/1 40 60 0.43 1/1 40 80 0.44 1/2 40 100 0.45 1/3 40 200 0.50 1/5 40 300 0.55 1/8 40 400 0.60 1/10 40 500 0.65 1/13 40 600 0.70 1/15 40 800 0.80 1/20 200 0 1.00 Microbial Qa Qb SI A/B C. albicans 10231 - PDB 0 600 1.00 (24 hours) 5 50 0.75 1/10 5 60 0.77 1/12 5 80 0.80 1/16 5 100 0.83 1/20 7.5 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 3000 1.00 (4 days > 10 1000 0.58 1/100 10 2000 0.92 1/200 20 1000 0.83 1/50 40 0 1.00 The ratio of ΒΒΙΤ/1-(3-chloroallyl)-3,5,7-triaza-1-nitrogenadamantane chloride measured ranged from 1/0.02 to 1/5000. 62 93646D17 201210506 24 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = dehydrogenation Acetic acid, sodium salt (SDHA) Microbial Qa Qb SI A/B E. coJi 8739 - M9GY 0 20000 1.00 (24 hours) 20 20000 1.10 1/1000 40 20000 1.20 1/500 60 20000 1.30 1/333 80 20000 1.40 1/ 250 100 20000 1.50 1/200 200 0 1.00 Microbial Qa Qb SI A/B C. albicans 10231 - PDB 0 40 1.00 (24 hours) 4 10 0.75 1/3 6 4 0.85 1/0.7 6 5 0.88 1/0.8 6 6 0.90 1/1 6 8 0.95 1/1 8 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 200 1.00 (7 days) 20 80 0.65 1/4 20 100 0.75 1/5 40 50 0.75 1/ 3 40 60 0.80 1/2 40 80 0.90 1/2 80 0 1.00 The ratio of BBIT/dehydroacetic acid (sodium salt) measured ranges from 1/0.02 to 1/5000. 63 93646D17 201210506 Table 25 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = sodium benzoate microorganism Qa Qb SI A/B E. coZi'8739 - M9GY 0 20000 1.00 (24 hours) 20 20000 1.10 1/1000 40 20000 1.20 1/500 60 20000 1.30 1/333 80 20000 1.40 1/250 100 20000 1.50 1/200 200 0 1.00 Microbial Qa Q«. SI A/B C. albicans 10231 - PDB 0 2000 1.00 (48 hours) 2.5 400 0.53 1/160 2.5 500 0.58 1/200 2.5 600 0.63 1/240 2.5 800 0.73 1/320 2.5 1000 0.83 ι/4〇σ 5 300 0.82 1/60 5 400 0.87 1/80 5 500 0.92 1/100 5 600 0.97 1/120 7.5 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 10000 1.00 (7 Day) 5 8000 0.86 1/1600 10 3000 0.43 1/300 10 4000 0.53 1/400 10 5000 0.63 1/500 10 6000 0.73 1/600 10 8000 0.93 1/800 15 3000 0.49 1/200 15 4000 0.59 1/267 15 5000 0.69 1/333 15 6000 0.79 1/400 15 7000 0.89 1/467 15 8000 0.99 1/533 64 93646D17 201210506

微生物 Qa Qb SI 20 1000 0.35 1/50 20 2000 0.45 1/100 20 3000 0.55 1/150 20 4000 0.65 1/200 20 5000 0.75 1/250 20 6000 0.85 1/300 20 7000 0.95 1/350 40 500 0.55 1/13 40 600 0.56 1/15 40 800 0.58 1/20 40 1000 0.60 1/25 40 2000 0.70 1/50 40 3000 0.80 1/75 40 4000 0.90 1/100 80 0 1.00 所測之ΒΒΠ7苯曱酸鈉之比率範圍自1/0.2至1/5000。 65 93646D17 201210506 表26 第一種成分(A)=N-(正-丁基)-1，2-苯并異噻唑啉酮(BBIT) 第二種成分(B)=擰檬酸鈉微生物 Q« Qb SI Am E. co/i 8739 - M9GY 0 20000 1.00 (24小時） 20 20000 1.10 1/1000 40 20000 1.20 1/500 60 20000 1.30 1/333 80 20000 1.40 1/250 . 100 20000 1.50 1/200 200 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 10000 1.00 (48小時） 2.5 3000 0.55 1/1200 2.5 4000 0.65 1/1600 2.5 5000 0.75 1/2000 2.5 6000 0.85 1/2400 5 600 0.56 1/120 5 800 0.58 1/160 5 1000 0.60 1/200 5 2000 0.70 1/400 5 3000 0.80 1/600 5 4000 0.90 1/800 10 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 10000 .1.00 (3天） 5 8000 0.93 1/1600 10 5000 0.75 1/500 10 6000 0.85 1/600 15 3000 0.68 1/200 15 4000 0.78 1/267 15 5000 0.88 1/333 15 6000 0.98 1/400 20 1000 0.60 1/50 20 2000 0.70 1/100 20 3000 0.80 1/150 66 93646D17 201210506 微生物 Q. Qb SI A/B 20 4000 0.90 1/200 40 0 1.00 所測之ΒΒΙΤ/檸檬酸鈉之比率範圍自1/0.2至1/5000。 67 93646D17 201210506 表27 第一種成分(A)= N-甲基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=N-(正-丁基)-1,2-苯并異噻唑啉酮(BBIT) 微生物 Qa Qb SI A/B E. coli 8739 - M9GY 0 100 1.00 (24小時） 2.5 50 0.75 1/20 2.5 60 0.85 1/24 5 40 0.90 1/8 7.5 10 0.85 1/1 7.5 20 0.95 1/3 10 0 1.00 微生物 Q. Qb SI A/B C. albicans 10231 - PDB 0 6 1.00 (48小時） 2.5 3 0.75 1/1 2.5 4 0.92 1/2 10 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 40 1.00 (4天） 10 5 0.38 1/0.5 10 6 0.40 1/0.6 10 8 0.45 1/0.8 10 10 0.50 1/1 10 20 0.75 1/2 20 6 0.65 1/0.3 20 8 0.70 1/0.4 20 10 0.75 1/0.5 40 0 1.00 所測之MBIT/BBIT之比率範圍自1/0.01至1/400。 68 93646D17 201210506 表28 第一種成分(A)= N-曱基-1，2-苯并異噻唑啉_3-酮(MBIT) 第二種成分(B)=氯化苯二曱烴銨（海亞敏（Hyamine) 3500) 微生物 Qa Qb SI ATR E. co/i 8739 - M9GY 0 10 1.00 (24小時） 2.5 4 1.25 1/2 5 3 1.25 1/0.6 7.5 3 1.50 1/0.4 100 0 1.00 微生物 Qa Qb SI A/B C albicans 10231 - PDB 0 60 1.00 (24小時） 2 10 0.42 1/5 2 20 0.58 1/10 2 30 0.75 1/15 2 40 0.92 1/20 4 3 0.55 1/0.8 4 4 0.57 1/1 4 5 0.58 1/1 4 6 0.60 1/2 4 8 0.63 1/2 4 10 0.67 1/3 4 20 0.83 1/5 6 3 0.80 1/0.5 6 4 0.82 1/0.7 6 5 0.83 1/0.9 6 6 0.85 1/1 6 8 0.88 1/1 6 10 0.92 1/2 8 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 200 1.00 (4天） 10 30 0.48 1/3 10 40 0.53 1/4 10 50 0.58 1/5 10 60 0.63 1/6 10 80 0.73 1/8 10 100 0.83 1/10 69 93646D17 201210506 微生物 Q. 0»» SI A/B 20 4 0.69 1/0.2 20 5 0.69 1/0.3 20 6 0.70 1/0.3 20 8 0.71 1/0.4 20 10 0.72 1/0.5 20 20 0.77 1/4 20 30 0.82 1/1.5 20 40 0.87 1/2 20 50 0.92 1/3 20 60 0.97 1/3 30 0 1.00 所測之MBIT/氣化苯二甲烴銨之比率範圍自1/0.01至 1/400。 70 93646D17 201210506 表29 第一種成分(A)= N-曱基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=氯化苯銨松寧（海亞敏1622) 微生物 Q- Qb SI A/B E. 8739 - M9GY 0 20 1.00 (24小時） 2.5 20 1.25 1/8 5 20 1.50 1/4 7.5 5 1.00 1/0.7 10 0 1.00 微生物 Q. Qb SI AfB C. albicans 10231 * PDB 0 4 1.00 (24小時） 2 3 1.00 1/2 4 3 1.25 1/0.8 6 1 1.00 1/0.2 8 0 1.00 微生物 Q. Qb SI A/B A, niger 16404 - PDB 0 10 1.00 (4天） 10 4 0.73 1/6.4 10 5 0.83 1/0.5 10 6 0.93 1/0.6 30 0 1.00 所測之MBIT/氯化苯銨松寧之比率範圍自1/0.01至1/400。 71 93646D17 201210506 表30 第一種成分(A)= N-甲基-1，2-苯并異噻唑啉-3 -酮(MBIT) 第二種成分(B)=苯甲醇微生物 Qa Qb SI A/B E. coJi8139 - M9GY 0 5000 1.00 (24小時） 2.5 5000 1.25 1/2000 5 4000 1.30 1/800 7.5 4000 1.55 1/533 10 0 1.00 — 微生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 4000 1.00 (48小時） 2.5 2000 0.75 1/800 5 500 0.63 1/100 5 1000 0.75 1/200 7.5 40 0.76 1/5 7.5 50 0.76 1/7 7.5 60 0.77 1/8 7.5 80 0.77 1/1 i 7.5 100 0.78 1/13 7.5 200 0.80 1/27 7.5 300 0.08 1/40 7.5 400 0.85 1/53 7.5 500 0.88 1/67 7.5 600 0.90 1/80 7.5 800 0.95 1/107 10 0 1.00 微生物 Q. Qb SI A/B A. niger 16404 - PDB 0 6000 1.00 (4天） 10 6000 1.20 1/600 20 6000 1.40 1/300 30 4000 1.27 1/133 50 0 1.00 ---- 所測之MBIT/苯曱醇之比率範圍自1/0.1至1/4000。 72 93646D17 201210506 表31 第一種成分(A)= N-甲基-1,2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=苯并異噻唑啉酮(BIT) 微生物 Qa Qb SI Am E. coli 8139 * M9GY 0 20 1.00 (24小時〉 2.5 10 0.75 1/4 5 5 0.75 1/1 5 6 0.80 1/1 5 8 0.90 1/2 7.5 3 0.90 1/0.4 7.5 4 0.95 1/0.5 10 0 1.00 微生物 Qa Qb SI Am C. albicans 10231 - PDB 0 30 1.00 (48小時） 2.5 10 0.58 1/4 2.5 20 0.92 1/8 5 4 0.63 1/0.8 5 5 0.67 1/1 5 6 0.70 1/1 5 8 0.77 1/2 5 10 0.83 1/2 7.5 2 0.82 1/0.3 7.5 3 0.85 1/0.4 7.5 4 0.88 1/0.5 7.5 5 0.92 1/0.7 7.5 6 0.95 1/0.8 10 0 1.00 微生物 Q· Qb SI Am A. niger 16404 - PDB 0 300 1.00 (7天） 10 100 0.53 1/10 10 200 0.87 1/20 20 30 0.50 1/2 20 40 0.53 1/2 20 50 0.57 1/3 20 60 0.60 1/3 20 80 0.67 1/4 20 100 0.73 1/5 73 93646D17 201210506 微生物 Qa Qb SI ΑΓΆ 30 20 0.67 1/0.7 30 30 0.70 1/1 30 40 0.73 1/1 30 50 0.77 1/2 30 60 0.80 1/2 30 80 0.87 1/3 30 100 0.93 1/3 50 0 1.00 所測之MBIT/苯并異噻唑啉酮之比率範圍自1/0.01至 1/400。 74 93646D17 201210506 表32 第一種成分(A)=N-曱基-1，2-笨并異噻唑啉-3-酮(MBIT) 第二種成分(B)=2-溴-2-硝基丙烷-1，3-二醇)(BNPD) 微生物 SI ： R co/f 8739-M9GY 024小時） ο 5 5 0 7 7 1 微生物 C. albicans 10231 - PDB (24小時）Microorganism Qa Qb SI 20 1000 0.35 1/50 20 2000 0.45 1/100 20 3000 0.55 1/150 20 4000 0.65 1/200 20 5000 0.75 1/250 20 6000 0.85 1/300 20 7000 0.95 1/350 40 500 0.55 1 /13 40 600 0.56 1/15 40 800 0.58 1/20 40 1000 0.60 1/25 40 2000 0.70 1/50 40 3000 0.80 1/75 40 4000 0.90 1/100 80 0 1.00 The measured ΒΒΠ7 sodium benzoate The ratio ranges from 1/0.2 to 1/5000. 65 93646D17 201210506 Table 26 First component (A) = N-(n-butyl)-1,2-benzisothiazolinone (BBIT) Second component (B) = sodium citrate microbe Q« Qb SI Am E. co/i 8739 - M9GY 0 20000 1.00 (24 hours) 20 20000 1.10 1/1000 40 20000 1.20 1/500 60 20000 1.30 1/333 80 20000 1.40 1/250 . 100 20000 1.50 1/200 200 0 1.00 Microbial Qa Qb SI A/B C. albicans 10231 - PDB 0 10000 1.00 (48 hours) 2.5 3000 0.55 1/1200 2.5 4000 0.65 1/1600 2.5 5000 0.75 1/2000 2.5 6000 0.85 1/2400 5 600 0.56 1 /120 5 800 0.58 1/160 5 1000 0.60 1/200 5 2000 0.70 1/400 5 3000 0.80 1/600 5 4000 0.90 1/800 10 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 10000 .1.00 (3 days) 5 8000 0.93 1/1600 10 5000 0.75 1/500 10 6000 0.85 1/600 15 3000 0.68 1/200 15 4000 0.78 1/267 15 5000 0.88 1/333 15 6000 0.98 1/400 20 1000 0.60 1/50 20 2000 0.70 1/100 20 3000 0.80 1/150 66 93646D17 201210506 Microorganism Q. Qb SI A/B 20 4000 0.90 1/200 40 0 1.00 / Na ratio of citrate range from 1 / 0.2 to 1/5000. 67 93646D17 201210506 Table 27 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = N-(n-butyl) -1,2-benzisothiazolinone (BBIT) Microbial Qa Qb SI A/B E. coli 8739 - M9GY 0 100 1.00 (24 hours) 2.5 50 0.75 1/20 2.5 60 0.85 1/24 5 40 0.90 1 /8 7.5 10 0.85 1/1 7.5 20 0.95 1/3 10 0 1.00 Microorganism Q. Qb SI A/B C. albicans 10231 - PDB 0 6 1.00 (48 hours) 2.5 3 0.75 1/1 2.5 4 0.92 1/2 10 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 40 1.00 (4 days) 10 5 0.38 1/0.5 10 6 0.40 1/0.6 10 8 0.45 1/0.8 10 10 0.50 1/1 10 20 0.75 1/2 20 6 0.65 1/0.3 20 8 0.70 1/0.4 20 10 0.75 1/0.5 40 0 1.00 The ratio of MBIT/BBIT measured ranges from 1/0.01 to 1/400. 68 93646D17 201210506 Table 28 First component (A) = N-mercapto-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = chlorinated benzodiazepine ( Hyamine 3500) Microorganism Qa Qb SI ATR E. co/i 8739 - M9GY 0 10 1.00 (24 hours) 2.5 4 1.25 1/2 5 3 1.25 1/0.6 7.5 3 1.50 1/0.4 100 0 1.00 Microorganisms Qa Qb SI A/BC albicans 10231 - PDB 0 60 1.00 (24 hours) 2 10 0.42 1/5 2 20 0.58 1/10 2 30 0.75 1/15 2 40 0.92 1/20 4 3 0.55 1/0.8 4 4 0.57 1/1 4 5 0.58 1/1 4 6 0.60 1/2 4 8 0.63 1/2 4 10 0.67 1/3 4 20 0.83 1/5 6 3 0.80 1/0.5 6 4 0.82 1/0.7 6 5 0.83 1/ 0.9 6 6 0.85 1/1 6 8 0.88 1/1 6 10 0.92 1/2 8 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 200 1.00 (4 days) 10 30 0.48 1/3 10 40 0.53 1/4 10 50 0.58 1/5 10 60 0.63 1/6 10 80 0.73 1/8 10 100 0.83 1/10 69 93646D17 201210506 Microorganism Q. 0»» SI A/B 20 4 0.69 1/0.2 20 5 0.69 1/0.3 20 6 0.70 1/0.3 20 8 0.71 1/0.4 20 10 0.72 1/0.5 20 20 0.77 1/4 20 30 0.82 1/1 .5 20 40 0.87 1/2 20 50 0.92 1/3 20 60 0.97 1/3 30 0 1.00 The ratio of MBIT/gasified benzalkonium chloride measured ranges from 1/0.01 to 1/400. 70 93646D17 201210506 Table 29 First component (A) = N-mercapto-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = Ammonium chloride chlorinated (sea) Yamin 1622) Microorganism Q- Qb SI A/B E. 8739 - M9GY 0 20 1.00 (24 hours) 2.5 20 1.25 1/8 5 20 1.50 1/4 7.5 5 1.00 1/0.7 10 0 1.00 Microbial Q. Qb SI AfB C. albicans 10231 * PDB 0 4 1.00 (24 hours) 2 3 1.00 1/2 4 3 1.25 1/0.8 6 1 1.00 1/0.2 8 0 1.00 Microbial Q. Qb SI A/BA, niger 16404 - PDB 0 10 1.00 (4 days) 10 4 0.73 1/6.4 10 5 0.83 1/0.5 10 6 0.93 1/0.6 30 0 1.00 The ratio of MBIT/ammonium chloride chlorinated was measured from 1/0.01 to 1/400. 71 93646D17 201210506 Table 30 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = benzyl alcohol microbe Qa Qb SI A/ B E. coJi8139 - M9GY 0 5000 1.00 (24 hours) 2.5 5000 1.25 1/2000 5 4000 1.30 1/800 7.5 4000 1.55 1/533 10 0 1.00 — Microbial Qa Qb SI A/B C. albicans 10231 - PDB 0 4000 1.00 (48 hours) 2.5 2000 0.75 1/800 5 500 0.63 1/100 5 1000 0.75 1/200 7.5 40 0.76 1/5 7.5 50 0.76 1/7 7.5 60 0.77 1/8 7.5 80 0.77 1/1 i 7.5 100 0.78 1/13 7.5 200 0.80 1/27 7.5 300 0.08 1/40 7.5 400 0.85 1/53 7.5 500 0.88 1/67 7.5 600 0.90 1/80 7.5 800 0.95 1/107 10 0 1.00 Microbial Q. Qb SI A/ B A. niger 16404 - PDB 0 6000 1.00 (4 days) 10 6000 1.20 1/600 20 6000 1.40 1/300 30 4000 1.27 1/133 50 0 1.00 ---- Measured MBIT/benzool ratio range From 1/0.1 to 1/4000. 72 93646D17 201210506 Table 31 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = Benzoisothiazolinone (BIT Microorganism Qa Qb SI Am E. coli 8139 * M9GY 0 20 1.00 (24 hours > 2.5 10 0.75 1/4 5 5 0.75 1/1 5 6 0.80 1/1 5 8 0.90 1/2 7.5 3 0.90 1/0.4 7.5 4 0.95 1/0.5 10 0 1.00 Microbial Qa Qb SI Am C. albicans 10231 - PDB 0 30 1.00 (48 hours) 2.5 10 0.58 1/4 2.5 20 0.92 1/8 5 4 0.63 1/0.8 5 5 0.67 1/1 5 6 0.70 1/1 5 8 0.77 1/2 5 10 0.83 1/2 7.5 2 0.82 1/0.3 7.5 3 0.85 1/0.4 7.5 4 0.88 1/0.5 7.5 5 0.92 1/0.7 7.5 6 0.95 1/0.8 10 0 1.00 Microbial Q· Qb SI Am A. niger 16404 - PDB 0 300 1.00 (7 days) 10 100 0.53 1/10 10 200 0.87 1/20 20 30 0.50 1/2 20 40 0.53 1/2 20 50 0.57 1/3 20 60 0.60 1/3 20 80 0.67 1/4 20 100 0.73 1/5 73 93646D17 201210506 Microorganism Qa Qb SI ΑΓΆ 30 20 0.67 1/0.7 30 30 0.70 1/1 30 40 0.73 1/1 30 50 0.77 1/2 30 60 0.80 1/2 30 80 0.87 1/3 30 100 0.93 1/3 50 0 1.00 The ratio of MBIT/benzisothiazolinone measured ranges from 1/0.01 to 1/400. 74 93646D17 201210506 Table 32 First component (A) = N-mercapto-1,2-p-isoisothiazoline -3-ketone (MBIT) second component (B) = 2-bromo-2-nitropropane-1,3-diol) (BNPD) microorganism SI: R co/f 8739-M9GY 024 hours) ο 5 5 0 7 7 1 Microorganism C. albicans 10231 - PDB (24 hours)

Qe ο 6 6 6 微生物 A. niger 16404 - PDB (4天）Qe ο 6 6 6 Microorganisms A. niger 16404 - PDB (4 days)

0 Q 0101020 8 1.00 5 0.88 1/2 1 0.63 1/0.2 2 0.75 1/0.4 3 0.88 1/0.6 1 0.88 1/0.1 2 1.00 1/0.3 0 1.00 » SI A/B 1000 1.00 100 0.60 1/25 200 0.70 1/50 300 0.80 1/75 400 0.90 1/100 4 0.75 1/0.7 5 0.76 1/0.8 6 0.76 1/1 8 0.76 1/1 10 0.76 1/2 20 0.77 1/3 30 0.78 1/5 40 0.79 1/7 50 0.80 1/8 60 0.81 1/10 80 0.83 1/13 100 0.85 1/17 200 0.95 1/33 0 1.00 \ SI A/B 2000 1.00 800 0.60 0/80 1000 0.70 1/100 5 0.40 1/0.3 93646D17 75 201210506 微生物 Q- Qb SI A/B 20 6 0.40 1/0.3 20 8 0.40 1/0.4 20 10 0.41 1/0.5 20 20 0.41 1/1 20 30 0.42 1/2 20 40 0.42 1/2 20 50 0.43 1/3 20 60 0.43 1/3 20 80 0.44 1/4 20 100 0.45 1/5 20 200 0.50 1/10 20 300 0.55 1/15 · 20 400 0.60 1/20 20 500 0.65 1/25 20 600 0.70 1/30 20 800 0.80 1/40 20 1000 0.90 1/50 30 0 1.00 所測之MBIT/2-溴-2-硝基丙烷-1，3-二醇之比率範圍自 1/0.01 至 1/400。 76 93646D17 201210506 表33 第一種成分(A)= N-曱基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)= 丁二醇微生物 Q. Qb SI A/B E. co7/8739 - M9GY 0 20000 1.00 (24小時） 2.5 20000 1.33 1/8000 5 20000 1.67 1/4000 7.5 0 1.00 微生物 Q. Qb SI A/B C. albicans 10231 · PDB 0 20000 1.00 (72小時） 8 100 0.81 1/13 8 200 0.81 1/25 8 300 0.82 1/38 8 400 0.82 1/50 8 500 0.83 1/63 8 600 0.83 1/75 8 800 0.84 1/100 8 1000 0.85 1/125 8 2000 0.90 1/250 8 3000 0.95 1/375 10 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 10000 1.00 — (7天） 10 10000 1.25 1/1000 20 10000 1.50 1/500 30 10000 1.75 1/333 40 0 1.00 — 所測之MBIT/丁二醇之比率範圍自1/0.1至1/4000。 77 93646D17 201210506 表34 第一種成分(A)= N-甲基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=辛醯二醇微生物 Qa Qb SI AJB K coJJ S139 M9GY 0 2000 1.00 (24小時） 2.5 2000 1.25 1/800 5 2000 1.50 1/400 7.5 2000 1.75 1/267 10 0 1.00 微生物 Qa Qb SI A/B G albicans 10231 - PDB 0 4000 1.00 (24小時） 2 2000 0.70 1/1000 2 3000 0.95 1/1500 4 2000 0.90 1/500 6 600 0.75 1/100 6 800 0.80 1/133 6 1000 0.85 1/167 8 200 0.85 1/25 8 300 0.88 1/38 8 400 0.90 1/50 8 500 0.93 1/63 8 600 0.95 1/75 10 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 1000 1.00 (4天） 10 1000 0.58 1/100 10 2000 0.83 1/200 20 1000 0.92 1/50 20 0 1.00 所測之MBIT/辛醯二醇之比率範圍自1/0.1至1/4000。 78 93646D17 201210506 表35 第一種成分(A)= N-曱基-1,2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)==氯苯甘鍵微生物 Qa Qb SI Am E. coJj 8739 - M9GY 0 2000 1.00 (24小時） 7.5 800 0.90 1/107 15 0 1.00 微生物 Q- Qb SI AfB C. albicans 10231 PDB 0 2000 1.00 (72小時） 4 200 0.50 1/50 4 300 0.55 1/75 4 400 0.60 1/100 4 500 0.65 1/125 4 600 0.70 1/150 4 800 0.80 1/200 4 1000 0.90 1/250 6 80 0.64 1/13 6 100 0.65 1/17· 6 200 0.70 1/33 6 300 0.75 1/50 6 400 0.80 1/67 6 500 0.85 1/83 6 600 0.90 1/100 8 40 0.82 1/5 8 50 0.83 1/6 8 60 0.83 1/8 8 80 0.84 1/10 8 100 0.85 1/13 8 200 0.90 1/25 8 300 0.95 1/38 10 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 2000 1.00 (3天） 10 2000 1.50 1/200 20 0 1.00 所測之MBIT/氣苯甘醚之比率範圍自1/0.1至1/4000。 79 93646D17 201210506 表36 第一種成分(Α)= Ν·甲基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=DMDM 乙内醯脲(Hydantoin) (DMDMH) 微生物 Q. Qb SI A/B E. coJj8139 - M9GY 0 50 1.0Q (24小時） 2.5 60 1.45 1/24 5 30 1.10 1/6 7.5 10 0.95 1/1 10 0 1.00 ..... 微生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 3000 1.00 (24小時） 2.5 3000 1.33 1/1200 5 1000 1.00 1/200 7.5 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 1000 1.00 (3天） 10 2000 2.25 1/200 20 2000 2.50 1/100 40 0 1.00 所測之MBIT/ DMDM乙内醯脲之比率範圍自1/0.1至 1/4000。MBIT及DMDM乙内醯脲間無觀察到協同作用。 80 93646D17 201210506 表37 第一種成分(A)= N-甲基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=二硫基-2,2’-雙(N-曱基苯曱醯胺)(DTBMA) 微生物 Qa Qb SI A/B E. coJi 8139 M9GY 0 80 1.00 (24小時） 2.5 20 0.42 1/8 2.5 30 0.54 1/12 2.5 40 0.67 1/16 2.5 50 0.79 1/20 2.5 60 0.92 1/24 5 20 0.58 1/4 5 30 0.71 1/6 5 40 0.83 1/8 5 50 0.96 1/10 7.5 3 0.54 1/0.4 7.5 4 0.55 1/0.5 7.5 5 0.56 1/0.7 7.5 6 0.58 1/0.8 7.5 8 0.60 1/1 7.5 10 0.63 1/1 7.5 20 0.75 1/3 7.5 30 0.88 1/4 10 1 0.68 1/0.1 10 2 0.69 1/0.2 10 3 0.70 1/0.3 10 4 0.72 1/0.4 10 5 0.73 1/0.5 10 6 0.74 1/0.6 10 8 0.77 1/0.8 10 10 0.79 1/1 10 20 0.92 1/2 15 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 * PDB 0 2000 1.00 (24小時） 6 60 0.63 1/10 6 80 0.64 1/13 81 93646D17 201210506 微生物 Qa Qb SI Am 6 100 0.65 1/17 6 200 0.70 1/33 6 300 0.75 1/50 6 400 0.80 1/67 6 500 0.85 1/83 6 600 0.90 1/100 8 10 0.81 1/1 8 20 0.81 1/3 8 30 0.82 1/4 8 40 0.82 1/5 8 50 0.83 1/6 8 60 0.83 1/8 8 80 0.84 1/10 8 100 0.85 1/13 8 200 0.90 1/25 8 300 0.95 1/38 10 0 1.00 微生物 Q. Qb SI Am A. niger 16404 * PDB 0 800 1.00 (3天） 10 500 0.96 1/50 30 0 1.00 所測之MBIT/二硫基-2,2’-雙(N-曱基苯曱醯胺）之比率範圍自 1/0.01 至 1/400。 82 93646D17 201210506 表38 第一種成分(A)= N-甲基-1，2-苯并異噻唑啉-3-酮（MBIT) 第二種成分(B)=乙二胺四乙酸(EDTA) 微生物 Q. Qb SI A/B E. coJi S789 - M9GY 0 40000 1.00 (24小時） 5 1600 0.54 1/320 10 0 1.00 微生物 Qa Qb SI A/B C: albicans 10231 · PDB 0 1000 1.00 (48小時） 2.5 60 0.43 1/24 2.5 80 0.47 1/32 2.5 100 0.50 1/40 2.5 120 0.53 1/48 7.5 0 1.00 微生物 Q. Qb SI A/B A. niger 16404 - PDB 0 1600 1.00 (3天） 10 800 0.75 1/80 10 1000 0.88 1/100 20 200 0.63 1/10 20 400 0.75 1/20 20 600 0.88 1/30 40 0 1.00 所測之MBIT/乙二胺四乙酸之比率範圍自1/0.3至1/8000。 83 93646D17 201210506 表39 第一種成分(A)= N-曱基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=對羥苯曱酸乙酯微生物 Q- Qb SI A/B E. coIi8139 - M9GY 0 2000 1.00 (24小時） 2.5 2000 1.25 1/800 5 2000 1.50 1/400 7.5 400 0.95 1/53 10 0 1.00 微生物 Q. Qb SI A/B C. albicans 10231 - PDB 0 2000 1.00 (24小時） 2.5 600 0.63 1/240 2.5 800 0.73 1/320 2.5 1000 0.83 1/400 2.5 2000 1.33 1/800 5 50 0.69 1/10 5 60 0.70 1/12 5 80 0.71 1/16 5 100 0.72 1/20 5 200 0.77 1/40 5 300 0.82 1/60 5 400 0.87 1/80 5 500 0.92 1/100 5 600 0.97 1/120 7.5 0 1.00 微生物 Q. Qb SI Am A. niger 16404 - PDB 0 800 1.00 (7天） 30 200 0.85 1/7 30 300 0.98 1/10 50 0 1.00 所測之MBIT/對羥苯曱酸乙酯之比率範圍自1/0.1至 1/4000。 84 93646D17 201210506 表40 第一種成分(A)=N-甲基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=己脒二羥乙基磺酸鹽微生物 Qa Qb SI Am E. coJi 8739 - M9GY 0 8 1.00 (24小時） 2.5 8 1.17 1/3 5 8 1.33 1/2 7.5 5 1.13 1/0.7 10 3 1.04 1/0.3 15 0 1.00 微生物 Qa Qb SI Am C. albicans 10231 - PDB 0 10 1.00 (24小時） 2 5 0.70 1/3 2 6 0.80 1/3 4 4 0.80 1/1 4 5 0.90 1/1 8 1 0.90 1/0:1 10 0 1.00 所測之MBIT/己脒二羥乙基磺酸鹽之比率範圍自1/0.01至 1/400。 85 93646D17 201210506 表41 第一種成分(A)= N-曱基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=雙辛氫啶0 Q 0101020 8 1.00 5 0.88 1/2 1 0.63 1/0.2 2 0.75 1/0.4 3 0.88 1/0.6 1 0.88 1/0.1 2 1.00 1/0.3 0 1.00 » SI A/B 1000 1.00 100 0.60 1/25 200 0.70 1/50 300 0.80 1/75 400 0.90 1/100 4 0.75 1/0.7 5 0.76 1/0.8 6 0.76 1/1 8 0.76 1/1 10 0.76 1/2 20 0.77 1/3 30 0.78 1/5 40 0.79 1/7 50 0.80 1/8 60 0.81 1/10 80 0.83 1/13 100 0.85 1/17 200 0.95 1/33 0 1.00 \ SI A/B 2000 1.00 800 0.60 0/80 1000 0.70 1/100 5 0.40 1/0.3 93646D17 75 201210506 Microorganism Q- Qb SI A/B 20 6 0.40 1/0.3 20 8 0.40 1/0.4 20 10 0.41 1/0.5 20 20 0.41 1/1 20 30 0.42 1/2 20 40 0.42 1/2 20 50 0.43 1/3 20 60 0.43 1/3 20 80 0.44 1/4 20 100 0.45 1/5 20 200 0.50 1/10 20 300 0.55 1/15 · 20 400 0.60 1/20 20 500 0.65 1/25 20 600 0.70 1/30 20 800 0.80 1/40 20 1000 0.90 1/50 30 0 1.00 The ratio of MBIT/2-bromo-2-nitropropane-1,3-diol measured ranges from 1/0.01 to 1 /400. 76 93646D17 201210506 Table 33 First component (A) = N-mercapto-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = butanediol microorganism Q. Qb SI A/B E. co7/8739 - M9GY 0 20000 1.00 (24 hours) 2.5 20000 1.33 1/8000 5 20000 1.67 1/4000 7.5 0 1.00 Microorganisms Q. Qb SI A/B C. albicans 10231 · PDB 0 20000 1.00 ( 72 hours) 8 100 0.81 1/13 8 200 0.81 1/25 8 300 0.82 1/38 8 400 0.82 1/50 8 500 0.83 1/63 8 600 0.83 1/75 8 800 0.84 1/100 8 1000 0.85 1/ 125 8 2000 0.90 1/250 8 3000 0.95 1/375 10 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 10000 1.00 — (7 days) 10 10000 1.25 1/1000 20 10000 1.50 1/500 30 10000 1.75 1/333 40 0 1.00 — The ratio of MBIT/butanediol measured ranges from 1/0.1 to 1/4000. 77 93646D17 201210506 Table 34 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = Octyl glycol microorganism Qa Qb SI AJB K coJJ S139 M9GY 0 2000 1.00 (24 hours) 2.5 2000 1.25 1/800 5 2000 1.50 1/400 7.5 2000 1.75 1/267 10 0 1.00 Microbial Qa Qb SI A/BG albicans 10231 - PDB 0 4000 1.00 (24 hours) 2 2000 0.70 1/1000 2 3000 0.95 1/1500 4 2000 0.90 1/500 6 600 0.75 1/100 6 800 0.80 1/133 6 1000 0.85 1/167 8 200 0.85 1/25 8 300 0.88 1/38 8 400 0.90 1/50 8 500 0.93 1/63 8 600 0.95 1/75 10 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 1000 1.00 (4 days) 10 1000 0.58 1/100 10 2000 0.83 1 /200 20 1000 0.92 1/50 20 0 1.00 The ratio of MBIT/octyl diol measured ranges from 1/0.1 to 1/4000. 78 93646D17 201210506 Table 35 First component (A) = N-mercapto-1,2-benzisothiazolin-3-one (MBIT) Second component (B) == Chlorophenyl glucoside microorganism Qa Qb SI Am E. coJj 8739 - M9GY 0 2000 1.00 (24 hours) 7.5 800 0.90 1/107 15 0 1.00 Microbial Q- Qb SI AfB C. albicans 10231 PDB 0 2000 1.00 (72 hours) 4 200 0.50 1/50 4 300 0.55 1/75 4 400 0.60 1/100 4 500 0.65 1/125 4 600 0.70 1/150 4 800 0.80 1/200 4 1000 0.90 1/250 6 80 0.64 1/13 6 100 0.65 1/17· 6 200 0.70 1/33 6 300 0.75 1/50 6 400 0.80 1/67 6 500 0.85 1/83 6 600 0.90 1/100 8 40 0.82 1/5 8 50 0.83 1/6 8 60 0.83 1/8 8 80 0.84 1/ 10 8 100 0.85 1/13 8 200 0.90 1/25 8 300 0.95 1/38 10 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 2000 1.00 (3 days) 10 2000 1.50 1/200 20 0 The ratio of MBIT/glycine to 1.00 measured ranges from 1/0.1 to 1/4000. 79 93646D17 201210506 Table 36 First component (Α) = Ν·Methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B)=DMDM Hydantoin (DMDMH) Microorganism Q. Qb SI A/B E. coJj8139 - M9GY 0 50 1.0Q (24 hours) 2.5 60 1.45 1/24 5 30 1.10 1/6 7.5 10 0.95 1/1 10 0 1.00 ..... Microorganism Qa Qb SI A/B C. albicans 10231 - PDB 0 3000 1.00 (24 hours) 2.5 3000 1.33 1/1200 5 1000 1.00 1/200 7.5 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 1000 1.00 (3 days) 10 2000 2.25 1/200 20 2000 2.50 1/100 40 0 1.00 The measured ratio of MBIT/DMDM to carbamide is from 1/0.1 to 1/4000. No synergistic effect was observed between MBIT and DMDM. 80 93646D17 201210506 Table 37 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = disulfo-2,2' - bis(N-mercaptophenylamine) (DTBMA) microorganism Qa Qb SI A/B E. coJi 8139 M9GY 0 80 1.00 (24 hours) 2.5 20 0.42 1/8 2.5 30 0.54 1/12 2.5 40 0.67 1 /16 2.5 50 0.79 1/20 2.5 60 0.92 1/24 5 20 0.58 1/4 5 30 0.71 1/6 5 40 0.83 1/8 5 50 0.96 1/10 7.5 3 0.54 1/0.4 7.5 4 0.55 1/0.5 7.5 5 0.56 1/0.7 7.5 6 0.58 1/0.8 7.5 8 0.60 1/1 7.5 10 0.63 1/1 7.5 20 0.75 1/3 7.5 30 0.88 1/4 10 1 0.68 1/0.1 10 2 0.69 1/0.2 10 3 0.70 1/0.3 10 4 0.72 1/0.4 10 5 0.73 1/0.5 10 6 0.74 1/0.6 10 8 0.77 1/0.8 10 10 0.79 1/1 10 20 0.92 1/2 15 0 1.00 Microbial Qa Qb SI A/B C. albicans 10231 * PDB 0 2000 1.00 (24 hours) 6 60 0.63 1/10 6 80 0.64 1/13 81 93646D17 201210506 Microorganism Qa Qb SI Am 6 100 0.65 1/17 6 200 0.70 1/33 6 300 0.75 1/ 50 6 400 0.80 1/67 6 500 0.85 1/83 6 600 0.90 1/100 8 10 0.81 1/1 8 20 0.81 1/3 8 30 0.82 1/4 8 40 0.82 1/5 8 50 0.83 1/6 8 60 0.83 1/8 8 80 0.84 1/10 8 100 0.85 1/13 8 200 0.90 1/25 8 300 0.95 1 /38 10 0 1.00 Microbial Q. Qb SI Am A. niger 16404 * PDB 0 800 1.00 (3 days) 10 500 0.96 1/50 30 0 1.00 MBIT/dithio-2,2'-double (N) The ratio of -mercaptobenzamine) ranges from 1/0.01 to 1/400. 82 93646D17 201210506 Table 38 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = ethylenediaminetetraacetic acid (EDTA) Microorganism Q. Qb SI A/B E. coJi S789 - M9GY 0 40000 1.00 (24 hours) 5 1600 0.54 1/320 10 0 1.00 Microbial Qa Qb SI A/BC: albicans 10231 · PDB 0 1000 1.00 (48 hours) 2.5 60 0.43 1/24 2.5 80 0.47 1/32 2.5 100 0.50 1/40 2.5 120 0.53 1/48 7.5 0 1.00 Microorganism Q. Qb SI A/B A. niger 16404 - PDB 0 1600 1.00 (3 days) 10 800 0.75 1/80 10 1000 0.88 1/100 20 200 0.63 1/10 20 400 0.75 1/20 20 600 0.88 1/30 40 0 1.00 The ratio of MBIT/ethylenediaminetetraacetic acid measured ranges from 1/0.3 to 1/1 8000. 83 93646D17 201210506 Table 39 First component (A) = N-mercapto-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = p-hydroxybenzoic acid ethyl ester microorganism Q- Qb SI A/B E. coIi8139 - M9GY 0 2000 1.00 (24 hours) 2.5 2000 1.25 1/800 5 2000 1.50 1/400 7.5 400 0.95 1/53 10 0 1.00 Microbial Q. Qb SI A/B C. Albicans 10231 - PDB 0 2000 1.00 (24 hours) 2.5 600 0.63 1/240 2.5 800 0.73 1/320 2.5 1000 0.83 1/400 2.5 2000 1.33 1/800 5 50 0.69 1/10 5 60 0.70 1/12 5 80 0.71 1/16 5 100 0.72 1/20 5 200 0.77 1/40 5 300 0.82 1/60 5 400 0.87 1/80 5 500 0.92 1/100 5 600 0.97 1/120 7.5 0 1.00 Microbial Q. Qb SI Am A. Niger 16404 - PDB 0 800 1.00 (7 days) 30 200 0.85 1/7 30 300 0.98 1/10 50 0 1.00 The ratio of MBIT/p-hydroxybenzoic acid ethyl ester measured ranges from 1/0.1 to 1/4000. 84 93646D17 201210506 Table 40 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = hexamethylene dihydroxyethane sulfonic acid Salt Microorganism Qa Qb SI Am E. coJi 8739 - M9GY 0 8 1.00 (24 hours) 2.5 8 1.17 1/3 5 8 1.33 1/2 7.5 5 1.13 1/0.7 10 3 1.04 1/0.3 15 0 1.00 Microbial Qa Qb SI Am C. albicans 10231 - PDB 0 10 1.00 (24 hours) 2 5 0.70 1/3 2 6 0.80 1/3 4 4 0.80 1/1 4 5 0.90 1/1 8 1 0.90 1/0:1 10 0 1.00 The ratio of MBIT/hexamethylene disulfonate was measured from 1/0.01 to 1/400. 85 93646D17 201210506 Table 41 First component (A) = N-mercapto-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = dioctylhydropyridine

微生物〇ϋ Qb SI E. coJi 8739 * M9GY (72小時）微生物 C. albicans 10231 * PDB (72小時）Microorganisms 〇ϋ Qb SI E. coJi 8739 * M9GY (72 hours) Microorganisms C. albicans 10231 * PDB (72 hours)

a Q 微生物 A. niger 16404 * PDB (3天）a Q Microorganism A. niger 16404 * PDB (3 days)

Qe 0 5 5 5 0222244446666888888810101015o1010 804050080405060803040506020304050568102030401230 14050 QbQbQe 0 5 5 5 0222244446666888888810101015o1010 804050080405060803040506020304050568102030401230 14050 QbQb

0 3 6 0 03333777700008913333890^,0,38 089·°·0·5·6·7·9·5·6·7·8·678·95·5·6·6·7·8·9·6·6·7·0^-·5·5 i0·0·1·1·0·0·0·0·0·0·0·0·0·0·0·0·0·0·0·0·0·0·0·0·0··0·1·1·0·0· SISI 1/8 1/10 1/20 1/25 1/30 1/40 1/8 1/10 1/13 1/15 1/3 1/5 1/7 1/8 1/0.6 1/0.7 1/1 1/1 1/3 1/4 1/5 1/0.1 1/0.2 1/0.3 1/4 1/5 86 93646D17 201210506 微生物 Qa Qb SI A/B 10 60 0.63 1/6 10 80 0.73 1/8 10 100 0.83 1/10 20 20 0.77 1/1 20 30 0.82 1/2 20 40 0.87 1/2 20 50 0.92 1/3 20 60 0.97 1/3 20 0 1.00 所測之MBIT/雙辛氫啶之比率範圍自1/0.01至1/400。 87 93646D17 201210506 表42 第一種成分(A)= N-甲基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=己二醇微生物 Q. Qb SI A/B B.coJj 8739-M9GY 0 20000 1.00 (24小時） 2.5 20000 1.17 1/8000 5 20000 1.33 1/4000 7.5 20000 1.50 1/2667 10 20000 1.67 1/2000 15 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 20000 1.00 (24小時） 2 20000 1.13 1/10000 4 20000 1.27 1/5000 6 20000 1.40 1/3333 8 20000 1.53 1/2500 10 20000 1.67 1/2000 15 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 20000 1.00 (3天） 10 20000 1.33 1/2000 20 20000 1.67 1/1000 30 0 1.00 所測之MBIT/己二醇之比率範圍自1/0.1至1/10000。MBIT 和己二醇間無觀察到協同作用。 88 93646D17 201210506 表43 第一種成分(A)=N-曱基-1,2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=對羥苯曱酸甲酯微生物 Qa Qb SI A/B E. co/j8739 - M9GY 0 2000 1.00 (24小時） 2.5 2000 1.25 1/800 5 2000 1.50 1/400 7.5 800 1.15 1/107 10 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 2000 1.00 (48小時） 2.5 1000 0.83 1/400 5 400 0.87 1/80 5 500 0.92 1/100 5 600 0.97 1/120 7.5 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 · PDB 0 800 1.00 (7天） 30 50 0.66 1/2 30 60 0.68 1/2 30 80 0.70 1/3 30 100 0.73 1/6 30 200 0.85 1/7 30 300 0.98 1/10 50 0 1.00 所測之MBIT/對羥苯曱酸甲酯之比率範圍自1/0.1至 1/4000。 89 93646D17 201210506 表44 第一種成分(A)=N-甲基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=戊二醇微生物 Qa Qb SI A/B R coJi 8739 - M9GY 0 20000 1.00 (24小時） 2.5 20000 1.17 1/8000 5 20000 1.33 1/4000 7.5 20000 1.50 1/2667 10 20000 1.67 1/2000 15 0 1.00 微生物 Q· Qb SI A/B C. albicans 10231 - PDB 0 20000 1.00 (24小時） 2 20000 1.13 1/10000 4 20000 1.27 1/5000 8 20000 1.53 1/2500 10 0 1.00 微生物 Q- Qb SI A/B A. niger 16404 - PDB 0 20000 1.00 (3天） 10 20000 1.67 1/2000 20 20000 2.33 1/1000 30 0 1.00 所測之MBIT/戍二醇之比率範圍自1/0.1至1/10000。MBIT 和戊二醇間無觀察到協同作用。 90 93646D17 201210506 表45 第一種成分(A)= N-甲基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=苯氧基乙醇微生物 Qa Qb SI AIB E. coJi 8139 * M9GY 0 4000 1.00 (24小時） 2.5 4000 1.17 1/1600 5 4000 1.33 1/800 7.5 4000 1.50 1/533 10 3000 1.42 1/300 40 3000 3.42 1/75 15 0 1.00 微生物 Qa Qb SI AfB C. albicans 10231 * PDB 0 3000 1.00 (24小時） 5 600 0.87 1/120 5 800 0.93 1/160 7.5 0 1.00 微生物 Qa Qb SI Am A niger 16404 - PDB 0 4000 1.00 (3天） 10 4000 1.25 1/400 20 2000 1.00 1/100 40 0 1.00 所測之MBIT/苯氧基乙醇之比率範圍自1/0.1至1/4000。 91 93646D17 201210506 表46 第一種成分(A)= N-甲基-1,2 -苯并異噻唑啉-3 -酮(MB IT) 第二種成分(B)=亞麻油醯胺丙基丙二醇-氯化二曱基銨磷酸酯（磷脂EFA) 微生物 Q. Qb SI AiB E. coA* 8739 - M9GY 0 500 1.00 (48小時） 15 40 0.83 1/3 15 50 0.85 1/3 15 60 0.87 1/4 15 80 0.91 1/5 15 100 0.95 1/7 20 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 1000 1.00 (24小時） 2 800 0.93 1/400 4 600 0.87 1/150 6 500 0.90 1/83 15 0 1.00 微生物 Q. Qb SI A/B A. niger 16404 - PDB 0 2000 1.00 (3天） 10 800 0.73 1/80 10 1000 0.83 1/100 20 300 0.82 1/15 20 400 0.87 1/20 20 500 0.92 1/25 20 600 0.97 1/30 30 0 1.00 所測之MBIT/亞麻油醯胺丙基丙二醇_氯化二曱基銨磷酸酯之比率範圍自1/0.01至1/400。 92 93646D17 201210506 表47 第一種成分(A)=N-甲基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=椰子油醯胺丙基丙二醇-氯化二曱基銨磷酸酯（磷脂PTC) 微生物 Qa Qb SI A/B E. coJi 8739 - M9GY 0 100 1.00 (48小時） 2.5 60 0.73 1/24 2.5 80 0.93 1/32 10 30 0.80 1/3 10 40 0.90 1/4 15 10 0.85 1/0.7 15 20 0.95 1/1 20 0 1.00 微生物 Q. Qb SI A/B C. albicans 10231 - PDB 0 1000 1.00 (24小時） 4 500 0.90 1/125 6 300 0.90 1/50 8 3 0.80 1/0.4 8 4 0.80 1/0:5 8 5 0.81 1/0.7 8 6 0.81 1/0.8 8 8 0.81 1/1 8 10 0.81 1/1 8 20 0.82 1/3 8 30 0.83 1/4 8 40 0.84 1/5 8 50 0.85 1/6 8 60 0.86 1/8 8 80 0.88 1/10 8 100 0.90 1/13 10 0 1.00 微生物 Qa Qb SI Am A. niger 16404 - PDB 0 1000 1.00 (4天） 10 500 0.83 1/50 10 600 0.93 1/60 30 0 1.00 所測之MBIT/椰子油醯胺丙基丙二醇-氣化二曱基銨磷酸酯之比率範圍自1/0.01至1/400。 93 93646D17 201210506 表48 第一種成分(A)= N-甲基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=山梨酸鉀微生物 Q· Qb SI A/B E. coJi 8139 - M9GY 0 20000 1.00 (48小時） 10 1000 0.72 1/100 10 2000 0.77 1/200 10 3000 0.82 1/300 10 4000 0.87 1/400 10 5000 0.92 1/500 10 6000 0.97 1/600 15 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 · PDB 0 400 1.00 (24小時） 2.5 200 0.83 1/80 5 40 0.77 1/8 5 50 0.79 1/10 5 60 0.82 1/12 5 80 0.87 1/16 5 100 0.92 1/20 7.5 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 10000 1.00 (4天） 10 6000 0.85 1/600 20 1000 0.60 1/50 20 2000 0.70 1/100 20 3000 0.80 1/150 20 4000 0.90 1/200 40 0 1.00 所測之MBIT/山梨酸鉀之比率範圍自1/0.1至1/4000。 94 93646D17 201210506 表49 第一種成分(A)= N-甲基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=對羥苯曱酸丙酯微生物 Qa Qb SI A/B K coJi 8139 - M9GY 0 4000 1.00 (48小時） 2.5 2000 0.67 1/800 2.5 3000 0.92 1/1200 5 2000 0.83 1/400 15 0 1.00 微生物 Q- Qb SI A/B C. albicans 10231 - PDB 0 2000 1.00 (24小時） 2.5 800 0.73 1/320 2.5 1000 0.83 1/400 5 400 0.87 1/80 5 500 0.92 1/100 5 600 0.97 1/120 7.5 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 2000 1.00 (7天） 10 800 0.60 1/80 10 1000 0.70 1/100 20 1000 0.90 1/50 30 300 0.75 1/10 30 400 0.80 1/13 30 500 0.85 1/17 30 600 0.90 1/20 50 0 1.00 所測之MBIT/對羥苯曱酸丙酯之比率範圍自1/0.1至 1/4000 〇 95 93646D17 201210506 表50 第一種成分(A)= N-曱基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=Quaternium-15(活化-1-(3-氯烯丙基)-3,5,7-三氮雜-1-氮鏽金剛烷氯化物）微生物 Q. Qb SI A/B E. coJJ 8739 - M9GY 0 50 1.00 (24小時） 5 20 0.90 1/400 7.5 2 0.79 1/0.3 7.5 3 0.81 1/0.4 7.5 4 0.83 1/0.5 7.5 5 0.85 1/0.7 7.5 6 0.87 1/0.8 7.5 8 0.91 1/1 7.5 10 0.95 1/1 10 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 500 1.00 (24小時） 2.5 500 1.33 1/200 5 300 1.27 1/60 7.5 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 - PDB 0 3000 1.00 (3天） 10 1000 0.58 1/100 10 2000 0.92 1/200 • 20 1000 0.83 1/50 40 0 1.00 所測之MBIT/1 -(3-氣烯丙基）_3，5,7-三氮雜-1 -氮鑌金剛烷氯化物之比率範圍自1/0.01至1/4000。 96 93646D17 201210506 表51 第一種成分(A)=N-甲基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=去氫乙酸，鈉鹽（SDHA) 微生物 Q· Qb SI AIB E. coh'8139 - M9GY 0 20000 1.00 (24小時） 2.5 20000 1.25 1/8000 5 20000 1.50 1/4000 7.5 20000 1.75 1/2667 10 0 1.00 微生物 Q. Qb SI AIB C. albicans 10231 · PDB 0 40 1.00 (24小時） 2 40 1.25 1/20 4 30 1.25 1/8 6 8 0.95 1/1 8 0 1.00 微生物 Q. Qb SI A/B A. niger 16404 - PDB 0 200 1.00 (7天） 20 80 0.80 1/4 20 100 0.90 1/5 30 50 0.85 1/2 30 60 0.90 1/2 50 0 1.00 所測之MBIT/去氫乙酸（鈉鹽）之比率範圍自1/0.01至 1/4000。 97 93646D17 201210506 表52 第一種成分(A)=N-曱基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=苯曱酸鈉微生物 Q. Qb SI A/B K coZf'8739 - M9GY 0 10000 1.00 (24小時） 2.5 10000 1.25 1/4000 5 10000 1.50 1/2000 7.5 10000 1.75 1/1333 10 0 1.00 微生物 Q- Qb SI A/B C. albicans 10231 - PDB 0 2000 1.00 (48小時） 2.5 800 0.65 1/320 2.5 1000· 0.75 1/400 5 300 0.65 1/60 5 400 0.70 1/80 5 500 0.75 1/100 5 600 0.80 1/120 5 800 0.90 1/160 7.5 30 0.77 1/4 7.5 40 0.77 1/5 7.5 50 0.78 1/7 7.5 60 0.78 1/8 7.5 80 0.79 1/11 7.5 100 0.80 1/13 7.5 200 0.85 1/27 7.5 300 0.90 1/40 7.5 400 0.95 1/53 10 0 1.00 微生物 Q. Qb SI A/B A niger 16404 - PDB 0 10000 1.00 (7天） 10 6000 0.77 1/600 10 8000 0.97 1/800 20 6000 0.93 1/300 30 2000 0.70 1/67 30 3000 0.80 1/100 30 4000 0.90 1/133 40 2000 0.87 1/50 98 93646D17 201210506 微生物 Qa Qb SI A/B 40 3000 0.97 1/75 60 0 1.00 所測之MBIT/苯甲酸鈉之比率範圍自1/0.1至1/4000。 99 93646D17 201210506 表53 第一種成分(A)= N-甲基-1，2-苯并異噻唑啉-3-酮(MBIT) 第二種成分(B)=擰檬酸鈉微生物 Qa Qb SI A/B R coIj S139 - M9GY 0 20000 1.00 (24小時） 2.5 20000 1.25 1/8000 5 20000 1.50 1/4000 7.5 20000 1.75 1/2667 10 0 1.00 微生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 10000 1.00 (24小時） 2.5 3000 0.63 1/1200 2.5 4000 0.73 1/1600 2.5 5000 0.83 1/2000 2.5 6000 0.93 1/2400 5 500 0.72 1/100 5 600 0.73 1/120 5 800 0.75 1/160 5 1000 0.77 1/200 5 2000 0.87 1/400 5 3000 0.97 1/600 7.5 0 1.00 微生物 Qa Qb SI A/B A. niger 16404 * PDB 0 10000 1.00 (7天） 30 1000 0.70 1/33 30 2000 0.80 1/67 30 3000 0.90 1/100 50 0 1.00 所測之MBIT/擰檬酸鈉之比率範圍自1/0.1至1/4000。 100 93646D17 201210506 表54 第一種成分(A)= N-甲基-1,2-苯并異嘆嗤琳_3_酮(MBIT) 第二種成分(B)=°比咬硫酮辞 E. coJi 8139 - M9GY (48小時） 0 2.5 2 0.4 1.00 0.45 1/0.2 2.5 0.5 0.50 1/0.2 2.5 0.6 0.55 1/0.2 2.5 0.8 0.65 1/0.3 2.5 1 0.75 1/0.4 5 0.3 0.65 1/0.06 5 0.4 0.70 1/0.08 5 0.5 0.75 1/0.1 5 0.6 0.80 1/0.1 5 0.8 0.90 1/0.2 7.5 0.3 0.90 1/0.04 7.5 0.4 0.95 1/0.05 10 0 1.00 徵生物 Qa Qb SI A/B C. albicans 10231 - PDB 0 30 1,00 (72小時） 2.5 4 0.47 1/2 2.5 5 0.50 1/2 2.5 6 0.53 1/2 2.5 8 0.60 1/3 2.5 10 0.67 1/4 5 2 0.73 1/0.4 5 3 0.77 1/0.6 5 4 0.80 1/0.8 5 5 0.83 1/1 5 6 0.87 1/1 5 8 0.93 1/2 7.5 0 1.00 A. niger 16404 * PDB 0 20 1.00 (3天） 20 5 0.75 1/3 20 6 0.80 1/0.3 20 8 0.90 1/0.4 40 0 1.00 所測之MBIT/吡啶硫酮鋅之比率範圍自1/〇〇〇1至1/400。 101 93646D17 201210506 【圖式簡單說明】無【主要元件符號說明】0 3 6 0 03333777700008913333890^,0,38 089·°·0·5·6·7·9·5·6·7·8·678·95·5·6·6·7·8·9·6· 6·7·0^-·5·5 i0·0·1·1·0·0·0·0·0·0·0·0·0·0·0·0·0·0·0·0 ·0·0·0·0·0··0·1·1·0·0· SISI 1/8 1/10 1/20 1/25 1/30 1/40 1/8 1/10 1/13 1/15 1/3 1/5 1/7 1/8 1/0.6 1/0.7 1/1 1/1 1/3 1/4 1/5 1/0.1 1/0.2 1/0.3 1/4 1/ 5 86 93646D17 201210506 Microorganism Qa Qb SI A/B 10 60 0.63 1/6 10 80 0.73 1/8 10 100 0.83 1/10 20 20 0.77 1/1 20 30 0.82 1/2 20 40 0.87 1/2 20 50 0.92 1/3 20 60 0.97 1/3 20 0 1.00 The ratio of MBIT/bisoctylhydropyridine measured ranges from 1/0.01 to 1/400. 87 93646D17 201210506 Table 42 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = hexanediol microorganism Q. Qb SI A/B B.coJj 8739-M9GY 0 20000 1.00 (24 hours) 2.5 20000 1.17 1/8000 5 20000 1.33 1/4000 7.5 20000 1.50 1/2667 10 20000 1.67 1/2000 15 0 1.00 Microbial Qa Qb SI A/B C. albicans 10231 - PDB 0 20000 1.00 (24 hours) 2 20000 1.13 1/10000 4 20000 1.27 1/5000 6 20000 1.40 1/3333 8 20000 1.53 1/2500 10 20000 1.67 1/2000 15 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 20000 1.00 (3 days) 10 20000 1.33 1/2000 20 20000 1.67 1/1000 30 0 1.00 The ratio of MBIT/hexanediol measured ranges from 1/0.1 to 1/10000 . No synergistic effect was observed between MBIT and hexanediol. 88 93646D17 201210506 Table 43 First component (A) = N-mercapto-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = Methylparaben Qa Qb SI A/B E. co/j8739 - M9GY 0 2000 1.00 (24 hours) 2.5 2000 1.25 1/800 5 2000 1.50 1/400 7.5 800 1.15 1/107 10 0 1.00 Microbial Qa Qb SI A/B C. Albicans 10231 - PDB 0 2000 1.00 (48 hours) 2.5 1000 0.83 1/400 5 400 0.87 1/80 5 500 0.92 1/100 5 600 0.97 1/120 7.5 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 · PDB 0 800 1.00 (7 days) 30 50 0.66 1/2 30 60 0.68 1/2 30 80 0.70 1/3 30 100 0.73 1/6 30 200 0.85 1/7 30 300 0.98 1/10 50 0 1.00 The ratio of MBIT/methylparaben is in the range of from 1/0.1 to 1/4000. 89 93646D17 201210506 Table 44 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = pentanediol microorganism Qa Qb SI A /BR coJi 8739 - M9GY 0 20000 1.00 (24 hours) 2.5 20000 1.17 1/8000 5 20000 1.33 1/4000 7.5 20000 1.50 1/2667 10 20000 1.67 1/2000 15 0 1.00 Microbial Q· Qb SI A/B C. Albicans 10231 - PDB 0 20000 1.00 (24 hours) 2 20000 1.13 1/10000 4 20000 1.27 1/5000 8 20000 1.53 1/2500 10 0 1.00 Microbial Q- Qb SI A/B A. niger 16404 - PDB 0 20000 1.00 ( 3 days) 10 20000 1.67 1/2000 20 20000 2.33 1/1000 30 0 1.00 The ratio of MBIT/decanediol measured ranges from 1/0.1 to 1/10000. No synergistic effect was observed between MBIT and pentanediol. 90 93646D17 201210506 Table 45 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = phenoxyethanol microorganism Qa Qb SI AIB E. coJi 8139 * M9GY 0 4000 1.00 (24 hours) 2.5 4000 1.17 1/1600 5 4000 1.33 1/800 7.5 4000 1.50 1/533 10 3000 1.42 1/300 40 3000 3.42 1/75 15 0 1.00 Microbial Qa Qb SI AfB C. albicans 10231 * PDB 0 3000 1.00 (24 hours) 5 600 0.87 1/120 5 800 0.93 1/160 7.5 0 1.00 Microbial Qa Qb SI Am A niger 16404 - PDB 0 4000 1.00 (3 days) 10 4000 1.25 1/400 20 2000 1.00 1/100 40 0 1.00 The ratio of MBIT/phenoxyethanol measured ranges from 1/0.1 to 1/4000. 91 93646D17 201210506 Table 46 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MB IT) Second component (B) = linseed oil allylpropyl propylene glycol - Dimethylammonium chloride phosphate (phospholipid EFA) Microorganism Q. Qb SI AiB E. coA* 8739 - M9GY 0 500 1.00 (48 hours) 15 40 0.83 1/3 15 50 0.85 1/3 15 60 0.87 1/ 4 15 80 0.91 1/5 15 100 0.95 1/7 20 0 1.00 Microbial Qa Qb SI A/B C. albicans 10231 - PDB 0 1000 1.00 (24 hours) 2 800 0.93 1/400 4 600 0.87 1/150 6 500 0.90 1/83 15 0 1.00 Microbial Q. Qb SI A/B A. niger 16404 - PDB 0 2000 1.00 (3 days) 10 800 0.73 1/80 10 1000 0.83 1/100 20 300 0.82 1/15 20 400 0.87 1 /20 20 500 0.92 1/25 20 600 0.97 1/30 30 0 1.00 The ratio of MBIT/linsein guanidinium propylene glycol dimethylammonium chloride phosphate was measured from 1/0.01 to 1/400. 92 93646D17 201210506 Table 47 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = coconut oil allylpropyl propylene glycol - Dimethyl ammonium phosphate (phospholipid PTC) microorganism Qa Qb SI A/B E. coJi 8739 - M9GY 0 100 1.00 (48 hours) 2.5 60 0.73 1/24 2.5 80 0.93 1/32 10 30 0.80 1/3 10 40 0.90 1/4 15 10 0.85 1/0.7 15 20 0.95 1/1 20 0 1.00 Microorganism Q. Qb SI A/B C. albicans 10231 - PDB 0 1000 1.00 (24 hours) 4 500 0.90 1/125 6 300 0.90 1/50 8 3 0.80 1/0.4 8 4 0.80 1/0:5 8 5 0.81 1/0.7 8 6 0.81 1/0.8 8 8 0.81 1/1 8 10 0.81 1/1 8 20 0.82 1/3 8 30 0.83 1/4 8 40 0.84 1/5 8 50 0.85 1/6 8 60 0.86 1/8 8 80 0.88 1/10 8 100 0.90 1/13 10 0 1.00 Microbial Qa Qb SI Am A. niger 16404 - PDB 0 1000 1.00 (4 days) 10 500 0.83 1/50 10 600 0.93 1/60 30 0 1.00 The ratio of MBIT/coconut oil guanidinium propyl propylene glycol-vaporized decyl ammonium phosphate was measured from 1/0.01 to 1 /400. 93 93646D17 201210506 Table 48 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = potassium sorbate microorganism Q· Qb SI A/B E. coJi 8139 - M9GY 0 20000 1.00 (48 hours) 10 1000 0.72 1/100 10 2000 0.77 1/200 10 3000 0.82 1/300 10 4000 0.87 1/400 10 5000 0.92 1/500 10 6000 0.97 1 /600 15 0 1.00 Microbial Qa Qb SI A/B C. albicans 10231 · PDB 0 400 1.00 (24 hours) 2.5 200 0.83 1/80 5 40 0.77 1/8 5 50 0.79 1/10 5 60 0.82 1/12 5 80 0.87 1/16 5 100 0.92 1/20 7.5 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 - PDB 0 10000 1.00 (4 days) 10 6000 0.85 1/600 20 1000 0.60 1/50 20 2000 0.70 1 /100 20 3000 0.80 1/150 20 4000 0.90 1/200 40 0 1.00 The ratio of MBIT/potassium sorbate measured ranges from 1/0.1 to 1/4000. 94 93646D17 201210506 Table 49 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = propyl paraben Qa Qb SI A/BK coJi 8139 - M9GY 0 4000 1.00 (48 hours) 2.5 2000 0.67 1/800 2.5 3000 0.92 1/1200 5 2000 0.83 1/400 15 0 1.00 Microbial Q- Qb SI A/B C. albicans 10231 - PDB 0 2000 1.00 (24 hours) 2.5 800 0.73 1/320 2.5 1000 0.83 1/400 5 400 0.87 1/80 5 500 0.92 1/100 5 600 0.97 1/120 7.5 0 1.00 Microbial Qa Qb SI A/B A . niger 16404 - PDB 0 2000 1.00 (7 days) 10 800 0.60 1/80 10 1000 0.70 1/100 20 1000 0.90 1/50 30 300 0.75 1/10 30 400 0.80 1/13 30 500 0.85 1/17 30 600 0.90 1/20 50 0 1.00 The ratio of MBIT/propylparaben measured ranges from 1/0.1 to 1/4000 〇95 93646D17 201210506 Table 50 First component (A) = N-mercapto-1 , 2-benzisothiazolin-3-one (MBIT) second component (B) = Quaternium-15 (activated-1-(3-chloroallyl)-3,5,7-triaza- 1-Nitrogen adamantane chloride) Microorganism Q. Qb SI A/B E. coJJ 8739 - M9GY 0 50 1.00 (24 hours) 5 20 0.90 1/400 7.5 2 0.79 1/0.3 7.5 3 0.81 1/0.4 7.5 4 0.83 1/0.5 7.5 5 0.85 1/0.7 7.5 6 0.87 1/0.8 7.5 8 0.91 1/1 7.5 10 0.95 1/1 10 0 1.00 Microbial Qa Qb SI A/B C. albicans 10231 - PDB 0 500 1.00 (24 hours) 2.5 500 1.33 1/200 5 300 1.27 1/60 7.5 0 1.00 Microbial Qa Qb SI A/B A . niger 16404 - PDB 0 3000 1.00 (3 days) 10 1000 0.58 1/100 10 2000 0.92 1/200 • 20 1000 0.83 1/50 40 0 1.00 MBIT/1 -(3-Allyl)_3 The ratio of 5,7-triaza-l-indenyl adamantane chloride ranges from 1/0.01 to 1/4000. 96 93646D17 201210506 Table 51 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = dehydroacetic acid, sodium salt (SDHA Microorganism Q· Qb SI AIB E. coh'8139 - M9GY 0 20000 1.00 (24 hours) 2.5 20000 1.25 1/8000 5 20000 1.50 1/4000 7.5 20000 1.75 1/2667 10 0 1.00 Microbial Q. Qb SI AIB C. Albicans 10231 · PDB 0 40 1.00 (24 hours) 2 40 1.25 1/20 4 30 1.25 1/8 6 8 0.95 1/1 8 0 1.00 Microorganism Q. Qb SI A/B A. niger 16404 - PDB 0 200 1.00 ( 7 days) 20 80 0.80 1/4 20 100 0.90 1/5 30 50 0.85 1/2 30 60 0.90 1/2 50 0 1.00 The ratio of MBIT/dehydroacetic acid (sodium salt) measured ranges from 1/0.01 to 1/4000. 97 93646D17 201210506 Table 52 First component (A) = N-mercapto-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = sodium benzoate microorganism Q. Qb SI A/BK coZf'8739 - M9GY 0 10000 1.00 (24 hours) 2.5 10000 1.25 1/4000 5 10000 1.50 1/2000 7.5 10000 1.75 1/1333 10 0 1.00 Microbial Q- Qb SI A/B C. albicans 10231 - PDB 0 2000 1.00 (48 hours) 2.5 800 0.65 1/320 2.5 1000· 0.75 1/400 5 300 0.65 1/60 5 400 0.70 1/80 5 500 0.75 1/100 5 600 0.80 1/120 5 800 0.90 1/ 160 7.5 30 0.77 1/4 7.5 40 0.77 1/5 7.5 50 0.78 1/7 7.5 60 0.78 1/8 7.5 80 0.79 1/11 7.5 100 0.80 1/13 7.5 200 0.85 1/27 7.5 300 0.90 1/40 7.5 400 0.95 1/53 10 0 1.00 Microbial Q. Qb SI A/BA niger 16404 - PDB 0 10000 1.00 (7 days) 10 6000 0.77 1/600 10 8000 0.97 1/800 20 6000 0.93 1/300 30 2000 0.70 1/ 67 30 3000 0.80 1/100 30 4000 0.90 1/133 40 2000 0.87 1/50 98 93646D17 201210506 Microbial Qa Qb SI A/B 40 3000 0.97 1/75 60 0 1.00 MBIT/sodium benzoate ratio measured Range from 1 / 0.1 to 1/4000. 99 93646D17 201210506 Table 53 First component (A) = N-methyl-1,2-benzisothiazolin-3-one (MBIT) Second component (B) = sodium citrate microbe Qa Qb SI A/BR coIj S139 - M9GY 0 20000 1.00 (24 hours) 2.5 20000 1.25 1/8000 5 20000 1.50 1/4000 7.5 20000 1.75 1/2667 10 0 1.00 Microbial Qa Qb SI A/B C. albicans 10231 - PDB 0 10000 1.00 (24 hours) 2.5 3000 0.63 1/1200 2.5 4000 0.73 1/1600 2.5 5000 0.83 1/2000 2.5 6000 0.93 1/2400 5 500 0.72 1/100 5 600 0.73 1/120 5 800 0.75 1/160 5 1000 0.77 1/200 5 2000 0.87 1/400 5 3000 0.97 1/600 7.5 0 1.00 Microbial Qa Qb SI A/B A. niger 16404 * PDB 0 10000 1.00 (7 days) 30 1000 0.70 1/33 30 2000 0.80 1/67 30 3000 0.90 1/100 50 0 1.00 The ratio of MBIT/sodium citrate measured ranges from 1/0.1 to 1/4000. 100 93646D17 201210506 Table 54 The first component (A) = N-methyl-1,2-benzopyranin _3_ketone (MBIT) The second component (B) = ° than the bite thione coJi 8139 - M9GY (48 hours) 0 2.5 2 0.4 1.00 0.45 1/0.2 2.5 0.5 0.50 1/0.2 2.5 0.6 0.55 1/0.2 2.5 0.8 0.65 1/0.3 2.5 1 0.75 1/0.4 5 0.3 0.65 1/0.06 5 0.4 0.70 1/0.08 5 0.5 0.75 1/0.1 5 0.6 0.80 1/0.1 5 0.8 0.90 1/0.2 7.5 0.3 0.90 1/0.04 7.5 0.4 0.95 1/0.05 10 0 1.00 Entity Qa Qb SI A/B C. albicans 10231 - PDB 0 30 1,00 (72 hours) 2.5 4 0.47 1/2 2.5 5 0.50 1/2 2.5 6 0.53 1/2 2.5 8 0.60 1/3 2.5 10 0.67 1/4 5 2 0.73 1/0.4 5 3 0.77 1 /0.6 5 4 0.80 1/0.8 5 5 0.83 1/1 5 6 0.87 1/1 5 8 0.93 1/2 7.5 0 1.00 A. niger 16404 * PDB 0 20 1.00 (3 days) 20 5 0.75 1/3 20 6 0.80 1/0.3 20 8 0.90 1/0.4 40 0 1.00 The ratio of MBIT/zinc pyrithione measured ranges from 1/〇〇〇1 to 1/400. 101 93646D17 201210506 [Simple description of the diagram] None [Key component symbol description]

Claims

201210506 VII. Patent application scope: 1 · An antibacterial composition comprising: (a) N-(n-butyl)-i, 2-benzisothiazoline _3 ketone; and (b) 1,3- Dihydroxyindolyl-5,5-dimethylhydantoin, wherein 'N-(n-butyl)-i,2-benzisothiazolin-3-one and 1,3-di-methyl The weight ratio of a 5,5-dimercaptoindolide is 〇. 〇2 to 1:200. 2' The antibacterial composition as described in claim 1, wherein N-(n-butyl)-1,2-benzopyranin β sits _3 嗣 and 1,3~ di fluorenyl The weight ratio of ~5,5-dimethylhydantoin is 1: 〇〇6 to 1:16 〇. 93646D17 201210506 IV. Designated representative map: (1) The representative representative figure of this case is: (). (There is no picture in this case) (2) A brief description of the symbol of the representative figure: (none) 5. If there is a chemical formula in this case, please disclose the chemical formula that best shows the characteristics of the invention: This case does not represent the chemical formula 2 93646D17