TW201105362A - Collagen production enhancer, and external composition for skin and cosmetic each comprising same - Google Patents
Collagen production enhancer, and external composition for skin and cosmetic each comprising same Download PDFInfo
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- TW201105362A TW201105362A TW099120421A TW99120421A TW201105362A TW 201105362 A TW201105362 A TW 201105362A TW 099120421 A TW099120421 A TW 099120421A TW 99120421 A TW99120421 A TW 99120421A TW 201105362 A TW201105362 A TW 201105362A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/16—Emollients or protectives, e.g. against radiation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/004—Aftersun preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
Description
201105362 六、發明說明: c發明戶斤屬之技術領域3 相關申請案之交互參照 本申請案係以已先行於日本提申之專利申請案特願 2009-148754號(申請日:2009年6月23日)為基礎,並主張其 優先權之利益,且其整體揭露内容係藉由交互參照而納入 於此。。 技術領域 本發明係有關於一種以燕窩之酵素分解物作為有效成 分之膠原蛋白產生促進劑。更詳而言之,本發明係有關於 一種可促進皮膚真皮纖維母細胞之I型膠原蛋白之產生之 膠原蛋白產生促進劑。進而,本發明係有關於一種包含前 述有效成分之燕窩之酵素分解物之皮膚外用組成物及化粧 品。 背景技術 肌膚乾燥、皺紋、肌膚鬆弛、彈性不佳等肌膚問題, 尤其受到女性重視與關心。該等肌膚問題通常被認為係由 環境因素、老化等增齡之因素、甚至壓力等心理因素等所 導致。尤其,近年紫外線增加及空調之泛用等所伴隨之生 活環境之乾燥化等所導致對肌膚造成不良影響之環境因素 可謂正逐漸增加。 皮膚組織係由表皮、真皮、皮下組織所構成。真皮含 有多量膠原蛋白及玻尿酸,而與皮膚之保水性及彈力息息201105362 VI. INSTRUCTIONS: c. Inventives in the field of technology 3 Inter-references to related applications This application is based on the patent application No. 2009-148754, which was first filed in Japan (application date: June 2009) On the basis of the 23rd, and claiming the benefits of its priority, and its overall disclosure is incorporated by reference. . BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a collagen production promoter which uses an enzyme decomposition product of bird's nest as an effective component. More specifically, the present invention relates to a collagen production promoter which promotes the production of type I collagen of skin dermal fibroblasts. Further, the present invention relates to a skin external composition and a cosmetic comprising an enzyme decomposition product of bird's nest containing the above-mentioned active ingredient. Background Art Skin problems such as dry skin, wrinkles, sagging skin, and poor elasticity are particularly valued and cared for by women. These skin problems are usually thought to be caused by environmental factors, ageing factors such as ageing, and even psychological factors such as stress. In particular, environmental factors that have adversely affected the skin caused by the increase in ultraviolet rays and the drying of the living environment in recent years have been gradually increasing. The skin tissue consists of epidermis, dermis, and subcutaneous tissue. The dermis contains a lot of collagen and hyaluronic acid, which is closely related to the water retention and elasticity of the skin.
S 3 201105362 相關。膠原蛋白及玻尿酸已知係由存在真皮中之真皮纖維 母細胞所產生。 據稱膠原蛋白約占人體内存在之蛋白質之30%,其中 70%存在於真皮中。存在真皮中之膠原蛋白雖包含I蜇膠原 蛋白及III型膠原蛋白,但主要則為I型膠原蛋白。以往,為 提昇肌膚彈性及光澤、皺紋之預防改善之目的,多採用以 外用方式使用添加有膠原蛋白之化粧品,而對皮膚直接補 充膠原蛋白之方法。然而,以外用方式補充膠原蛋白時, 不易使皮膚真皮之膠原蛋白增加,故難謂絕對充分可使肌 膚之彈性及光澤提昇、預防改善皺紋。 皮膚若曝曬於包含紫外線之光線下,已知占真皮之細 胞外基質之大半之膠原蛋白將顯著減少,並使其纖維束排 列不規則。因此’真皮之膠原蛋白量增加有助於皺紋之改 善及預防等(文獻1「用於機能性化粧品材料之開發之實驗 法」(芋皮玄爾監修),2007年發行,CMC出版,第45〜47 頁)。又,其中亦揭露中波紫外線(UVB)之照射將使真皮纖 維母細胞之膠原蛋白合成大幅減少。其中,〗型膠原蛋白合 成促進劑係例示為維他命C誘導體、乳清成分等。 另’文獻 2 (Akiko Kojima-Yuasa, et al.,Fragrance Journal, April, 2009, No. 346 (Vol.37, No. 4), pp. l〇3-)t UJ 揭露了膠原蛋白產生能力之降低之抑制有助於Q〇L之提昇 改善,皮膚之膠原蛋白量之降低則為皺紋及鬆弛等之成因。 I型膠原蛋白之產生促進劑迄今已有著眼於特定之植 物來源成分而加以利用之多數報告(諸如特開2〇〇8_丨〇5984 4 201105362 號公報及W02004/085429)。 燕窩係金絲燕以自己的唾液構成絲狀而築成之巢,已 知其成分包含多量之酷蛋白質’而幾乎不含脂質。燕寫迄 今使用於中菜作為食材’亦為中藥之原料等。近年,亦已 開發以燕窩為原料之健康食品等,至於外用劑及化粧品等 外用用途之應用例則尚少為人知。 特開2003-95961號公報中已揭露使用燕窩之酵素分解 物之口服用之美肌促進劑。然而,其中僅揭露口服用途, 而未就外用用途之利用可能性作任何揭露及暗示。又,其 效果係肌膚保濕感之提昇及肌理細緻肌膚之實現,並未就 可提昇肌膚彈性及光澤並改善皺紋之I型膠原蛋白之產生 促進能力作任何揭露。 WO99/022709中,已揭露包含燕窩之含水溶劑萃取 物’特別是熱水萃取物之化粧品。該化粧品可使用於皮膚 保養用途,係使用於外用用途者。又,其中亦揭露燕窩之 含水萃取物具有皮膚細胞之膠原蛋白合成促進效果。然 而’其使用之萃取物係就燕窩加以熱水萃取而去除固態部 分而成者,與就燕窩整體進行酵素處理而加以直接使用之 燕窩酵素分解物顯然不同。又,關於其效果,依本發明人 等之考量認為仍有改善之空間。 【号务明内3 發明概要 本發明人此次發現了燕窩之酵素分解物具有皮膚真皮 纖維母細胞之I型膠原蛋白之優良產生促進效果。其效果較S 3 201105362 Related. Collagen and hyaluronic acid are known to be produced by dermal fibroblasts present in the dermis. Collagen is said to account for about 30% of the protein in the body, 70% of which is found in the dermis. Collagen in the dermis contains collagen I and collagen type III, but mainly type I collagen. In the past, in order to improve the skin's elasticity, luster, and wrinkles, it is common to use a collagen-added cosmetic for external use and a method of directly supplementing the skin with collagen. However, when collagen is added by external means, it is difficult to increase the collagen of the skin dermis, so it is difficult to increase the elasticity and gloss of the skin and prevent wrinkles. If the skin is exposed to light containing ultraviolet light, it is known that most of the collagen in the extracellular matrix of the dermis will be significantly reduced and the fiber bundles will be irregularly arranged. Therefore, the increase in the amount of collagen in the dermis contributes to the improvement and prevention of wrinkles (Document 1 "Experimental method for the development of functional cosmetic materials" (Supervised by Suo Xuan Xuan), issued in 2007, published by CMC, No. 45 ~47 pages). In addition, it is also revealed that ultraviolet light (UVB) irradiation will greatly reduce the collagen synthesis of dermal fibroblasts. Among them, the collagen-type synthesis promoter is exemplified by a vitamin C inducer, a whey component, and the like. Another 'Document 2 (Akiko Kojima-Yuasa, et al., Fragrance Journal, April, 2009, No. 346 (Vol. 37, No. 4), pp. l〇3-)t UJ reveals collagen production ability The reduction of the inhibition contributes to the improvement of Q〇L, and the decrease in the amount of collagen in the skin is caused by wrinkles and slacks. The type I collagen production promoter has hitherto been reported to have a large number of reports on the use of specific plant-derived components (such as Japanese Patent Laid-Open No. Hei 2, No. 5,984, No. 4, 2011, 052, and WO2004/085429). The swallow's nest swiftlets form a nest of filaments in their own saliva, and it is known that their constituents contain a large amount of cool protein' and are almost free of lipids. Yan wrote that it is used in Chinese cuisine as a raw material. It is also a raw material for traditional Chinese medicine. In recent years, health foods such as bird's nest have been developed, and applications for external use such as external preparations and cosmetics are still less well known. A cosmetic agent for oral use using an enzyme decomposition product of bird's nest has been disclosed in Japanese Laid-Open Patent Publication No. 2003-95961. However, only oral use is disclosed therein, and no disclosure or suggestion is made as to the possibility of use for external use. Moreover, the effect is that the moisturizing effect of the skin and the realization of the delicate skin of the skin are not disclosed in the ability to promote the growth and elasticity of the skin and to improve the production of type I collagen of wrinkles. In WO 99/022709, a cosmetic comprising an aqueous solvent extract of bird's nest, in particular a hot water extract, has been disclosed. The cosmetic can be used for skin care purposes and is used for external use. Further, it is also revealed that the aqueous extract of bird's nest has a collagen synthesis promoting effect on skin cells. However, the extracts used in the bird's nest are extracted by hot water extraction to remove the solid portion, and the bird's nest enzyme decomposition products which are directly used for the enzyme treatment of the bird's nest are obviously different. Further, regarding the effect, it is considered that there is still room for improvement in consideration of the present inventors. [Immediately, the present invention has found that the enzyme decomposition product of bird's nest has an excellent production-promoting effect of type I collagen of skin dermal fibroblasts. Its effect is better
S 201105362 燕窩之熱水萃取物之效果更為顯著,又 高之酵素分解物之上述效果則更為明顯。本:: 發現為依據。 月即以i玄荨 較二目的在提供可有效率地生產且安全性 :=㈣蛋白之產生促進㈣含前者之外用 作::膠原蛋白產生促進劑―酵素分解物 依據本發月之—較佳態樣,前述酵素分解㈣蛋白酶 之分解物。 更佳態樣,前述酵素分解物之分子量 依據本發明之 為 70〜200,000。 依據本發明之~最佳態樣,前述酵素分解物之平均分 子量為300〜15,00〇。 依據本發明之較佳態樣,本發明之膠原蛋白產生促進 劑可促進皮膚真皮纖維母細胞之原蛋白產生。 依據本U之其它態樣,可提供— 物,其包含本發明之膠眉^ 產生促_。餘成物宜使 用於皮膚保養’更宜使用於提昇肌膚之彈性與光澤 預防或改善皺紋。 U之其匕更佳態樣本發明之皮 物係化粧品。 /风 據本發月之其它態樣,可提供-種燕窩之酵素分解 物之用途其細於製造涵括人類在内之錢動物之皮膚 201105362 之膠原蛋白產生促進劑。依據本發明之另—態樣,可提供 -種燕窩之酵素分解物之用途,其仙於製造皮膚保養用 之皮膚外用組成物。 進而,依據本發明之其它態樣,可提供一種皮膚之膠 原蛋白之產生促進方法,包含:將有效量之本發明有效成 分之酵素分解物制於涵括人類在内之哺乳祕之皮膚。 依據本發明之另-態樣’可提供—種皮膚保養方法包含: 將有效量之本發财效成分應用於涵括人類在内之鳴乳動 物之皮膚上。同樣地,亦可提供—種方法,包含:將有效 里之本發明有效成分制於涵括人類在内之嗜乳動物之皮 膚上;其可提昇肌膚之彈性與光澤,並預防或改善皴紋。 依據本發明,使用燕高之酵素分解物作為有效成分, 可較以往更為顯著地促進人類皮膚真皮纖維母細胞之勝原 蛋白之產生。X,基於上述真皮纖維母細胞之膠原蛋白之 產生促進能力,而將酵素分解物使用於皮膚外用用途或化 粧品用途,則可提昇肌膚之彈性(彈力)’並增加肌膚光澤, 進而預防或減少皺紋。進而,可使日曬後肌膚在膠原蛋白 合成減少之狀態下促進增生膠原蛋白,而提昇肌膚之彈性 及光澤,並進行敵紋之改善。如此’依據本發明,可實現 消費者之Q〇L之提昇。X,所使用之酵素分解物係使:亦 公知為食材之燕窩,故安全性較高,消費者接受度亦較高。 進而’酵素分解物可直接就燕窩進行酵素處理而取得,故 可有效率進行製造’且降低製造成本。 圖式簡單說明S 201105362 The effect of the hot water extract of bird's nest is more pronounced, and the above effect of the high enzyme decomposition product is more obvious. Ben:: Discovery is the basis. In the month of i Xuanqi, the purpose is to provide efficient production and safety: = (4) protein production promotion (4) for use in addition to the former:: collagen production promoter - enzyme decomposition products according to this month - compared In the best case, the aforementioned enzyme decomposes (4) a decomposition product of the protease. More preferably, the molecular weight of the aforementioned enzyme decomposition product is 70 to 200,000 in accordance with the present invention. According to a preferred embodiment of the present invention, the average molecular weight of the aforementioned enzyme decomposition product is 300 to 15,00 Å. According to a preferred embodiment of the present invention, the collagen production promoting agent of the present invention can promote the production of protoproteins of dermal fibroblasts. According to other aspects of the present invention, there is provided a substance comprising the gel eyebrow of the present invention. Residue should be used for skin care. It is more suitable for improving the elasticity and luster of the skin to prevent or improve wrinkles. U's better sample of the skin of the invention. /Wind According to other aspects of this month, the use of the enzyme decomposition of bird's nest can be provided to produce a collagen production promoter for the skin of animal animals including humans. According to another aspect of the present invention, there is provided a use of an enzyme decomposition product of bird's nest, which is useful for producing a skin external composition for skin care. Further, according to another aspect of the present invention, there is provided a method for promoting the production of a collagen protein of the skin comprising: an effective amount of an enzyme decomposition product of the present invention, which is applied to a skin of a mammal which includes humans. A skin care method according to another aspect of the present invention may comprise: applying an effective amount of the present intensive ingredient to the skin of a milking animal including humans. Similarly, a method can be provided comprising: preparing an effective active ingredient of the present invention on the skin of a milk-producing animal including humans; it can enhance the elasticity and luster of the skin, and prevent or improve the crepe . According to the present invention, the use of the enzyme decomposition product of Yangao as an active ingredient can more significantly promote the production of the primordial protein of human skin dermal fibroblasts than in the past. X, based on the collagen production promoting ability of the above dermal fibroblasts, and the use of the enzyme decomposition product for external use or cosmetic use of the skin can enhance the elasticity (elasticity) of the skin and increase the skin's luster, thereby preventing or reducing wrinkles. . Further, it is possible to promote the proliferation of collagen in the state where the collagen synthesis is reduced after the sun exposure, thereby enhancing the elasticity and luster of the skin and improving the enemy pattern. Thus, according to the present invention, the improvement of the consumer's Q〇L can be achieved. X, the enzyme decomposition product used is: It is also known as the bird's nest of the foodstuff, so the safety is high and the consumer acceptance is also high. Further, the enzyme decomposition product can be obtained by directly performing enzyme treatment on the bird's nest, so that it can be efficiently manufactured and the manufacturing cost can be reduced. Simple illustration
S 201105362 第i®係顯示燕窩樣本中之涎酸量之測定結果之圖表。 第2圖係顯示燕窩樣本中之蛋白質量之測定結果之圖 表。 第3圖係顯示採GP C - ΗP L C之燕窩酵素分解物1之測定 結果之圖表。 第4圖係顯示採GPC-HPLC之燕窩酵素分解物2之測定 結果之圖表。 第5圖係|員示膠原蛋白之產生促進能力之測定結果之 圖表。 發明之具體說明 有效成分 本發明之膠原蛋白產生促進劑一如前述,係以燕窩之 酵素分解物作為有效成分。 酵素分解物之原料燕窩係金絲燕將自己的唾液構成絲 狀而築成之巢’其成分包含多量之醣蛋白質,幾乎不含有 脂質。在此,金絲燕係指雨燕科(Apodidae)之金絲燕屬 (Collocalia)下之燕類,可例舉諸如小灰腰金絲燕(Ap〇didae Collocalia francica)、苔巢金絲燕(Apodidae Collocalia salanga)、爪唾金絲燕(Apodidae Collocalia fuciphaga)、印度 食用巢金絲燕(Apodidae Collocalia inexpectat)、褐腰金絲燕 (Apodidae Collocalia vestita)、白腹金絲燕(Apodidae Collocalia esculent)、大金絲燕(Apodidae Collocalia maximus)等。 201105362 本發明中’燕窩可使用市售品。一般市售之燕窩從僅 去除燕毛及糞便等汙垢而經清洗者,至收集燕窩之碎屑而 反覆進行你白與清洗而成形者,涵括多種各類,但本發明 所使用之燕窩則宜使用未在前置處理時經過度清洗及漂白 等之燕窩。 ”、'窩之酵素分解物係指就燕窩進行酵素反應加以分解 所知·者,可使用蛋白酶或含有蛋白酶之複合酵素等分解燕 寫或其處理物而製得。在此,分解處理所使用之酵素宜為 蛋白酶,使用胰酶則更佳。 燕窩之酵素分解物通常可先經加熱殺菌處理,然後於 水中進行酵钱理。㈣處理之條件可適t參考慣用之加 熱殺菌條件㈣定,酵讀理之㈣亦可制所使用之酵 素之種類及燕窩之狀態而適當加以設定。 更具體之例,則可依以下程糊製燕窩之酵素分解物。 舉例言之,對已磨碎之燕窩加入其質量之1〇〜5〇倍之水 而使其膨脹後’在60〜UGtT進行加熱_處理5秒至3〇 分鐘。然後’直接添加適量之酵素,而在酵素之最佳阳、 最佳溫度下進行酵素反應〇·5〜48小時,素反應結束後, 藉加熱處理粒酵素不活性化,並過敍應液W除不溶 物、’即可製得燕窩之酵素分解物。又,上述酵素分解物亦 可視需要而乾践錄1,上述各料巾,亦可適當進 行pH調整、脫色、脫臭等操作。 他例則在就燕寫之處理物加以酵素分解時,就對粒徑 2麵以下、150叫以下更佳之已磨碎之燕寫加入其質量: 9S 201105362 The i® series shows a graph showing the results of the determination of the amount of tannic acid in the bird's nest samples. Figure 2 is a graph showing the results of the determination of the amount of protein in the bird's nest samples. Fig. 3 is a graph showing the results of measurement of the bird's nest enzyme decomposition product 1 of GP C - ΗP L C . Fig. 4 is a graph showing the results of measurement of the bird's nest enzyme decomposition product 2 by GPC-HPLC. Fig. 5 is a graph showing the results of measurement of the ability to promote collagen production. DETAILED DESCRIPTION OF THE INVENTION Active ingredient The collagen production promoter of the present invention, as described above, is an enzyme decomposition product of bird's nest as an active ingredient. The raw material of the enzyme decomposition product, the swiftlet swallows form a nest of their saliva and form a nest. The ingredients contain a large amount of glycoprotein and contain almost no lipids. Here, the swiftlet refers to the swallows under the genus Collocalia of Apodidae, and may be exemplified by ApAdidae Collocalia francica, Moss Nest swiftlet ( Apodidae Collocalia salanga), Apodidae Collocalia fuciphaga, Apodidae Collocalia inexpectat, Apodidae Collocalia vestita, Apodidae Collocalia esculent, Apodidae Collocalia maximus and the like. 201105362 In the present invention, a commercially available product can be used for the bird's nest. Generally, the bird's nest that is commercially available from the cleaning of only the dirt such as swallows and feces, to the collection of the waste of the bird's nest, and the white and cleansing of the bird's nest, includes a variety of types, but the bird's nest used in the present invention is It is advisable to use bird's nest that has not been cleaned and bleached prior to pretreatment. "The enzyme decomposition product of the nest is known to be decomposed by the enzyme reaction of the bird's nest. It can be obtained by decomposing swallowing or its treatment with a protease or a compound enzyme containing a protease. Here, the decomposition treatment is used. The enzyme is preferably a protease, and it is better to use pancreatic enzyme. The enzyme decomposition product of bird's nest can usually be heat-sterilized and then fermented in water. (4) The conditions of treatment can be adjusted according to the conventional heat sterilization conditions (4). The yeast (4) can also be appropriately set according to the type of enzyme used and the state of the bird's nest. More specifically, the enzyme decomposition of bird's nest can be pasteed in the following way. For example, the ground is broken. The bird's nest is added with 1质量~5〇 times the mass of the water to make it swell and then heated at 60~UGtT_treatment for 5 seconds to 3 minutes. Then 'add the right amount of enzyme directly, and the best yang in the enzyme, The enzyme reaction is carried out at the optimum temperature for 5 to 48 hours. After the end of the reaction, the granzyme is inactivated by heat treatment, and the insoluble matter is removed by the solution W, and the enzyme decomposition product of the bird's nest can be obtained. The above-mentioned enzyme decomposition products can also be processed as needed. The above-mentioned materials can also be subjected to pH adjustment, decolorization, deodorization, etc. In other cases, when the enzyme is decomposed by the treatment of the Swallow, the particles are granulated. Below the 2nd surface, 150 is better than the grated swallow to write its quality: 9
S 201105362 10~100倍之水’並在ΜΟΙ下予以靜置或攪拌〇5〜48小時 而使其膨服後’在60〜130°c下進行加熱殺菌處理5秒〜30分 鐘’並視需要進行過濾而製得溶液,再與上述同樣施以酵 素處理,而可製得燕窩之酵素分解物。 酵素分解物之調製法係酵素反應之時間愈長,蛋白質 之分解私度愈面’而可獲致包含更低分子之蛋白質之酵素 分解物。本發明中,酵素反應時間為〇 5〜48小時,而3〜36 小時更佳,約24小時程度最佳,並宜使用酵素反應較充分 進行後之酵素分解物。 如此製得之燕窩之酵素分解物之分子量宜為 70〜200,000,70〜180,000則更佳。分子量為7〇〜15〇 〇〇〇則極 佳,70〜120,000則最佳。 又,热窩之酵素分解物之平均分子量(重量平均分子量) 宜為300〜1〇〇,〇〇0,而以3〇〇〜7〇 〇〇〇為更佳。平均分子量 300〜50,000則甚佳,3〇〇〜30,000為極佳,3〇〇〜2〇〇〇〇再更 佳,300〜15,000則最佳。 皮膚外用組成物 本發明之膠原蛋白產生促進劑雖可單獨直接使用,但 亦可内含於純品m準_等各種皮料用組成 物中作為添加劑,而製得具有膠原蛋白產生促進效果之組 成物。所製得之組成物可有效使用於皮膚保養,更宜用於 提昇肌膚之彈性與光澤及預防或改善敏紋。 本發明之有效成分之燕寫之酵素分解物具有人類皮膚 真皮纖維母細胞之〖型膠原蛋白之產生促進活性(後述實施 201105362 例之測試結果)。 因此’本發明中,所謂膠原蛋白之產生促進活性意指 促進或改善人類皮膚真皮纖維母細胞之I型膠原蛋白之產 生能力之作用,意在藉此可增加人類皮膚真皮中之膠原蛋 白量。增加真皮之膠原蛋白量,即可獲致皺紋改善及預防 效果,並可提昇肌膚彈性(彈力)且增加肌膚之光澤,本業之 技術人員尤已周知(諸如前述之文獻(「用於機能性化粧品材 料之開發之貫驗法」(芋皮玄爾監修),2〇〇7年發行,CMC 出版’第45〜47頁(非專利文獻1))及文獻(Akiko Kojima-Yuasa, et al., Fragrance Journal, April, 2009, No. 346 (Vol.37, No. 4),pp. 103_ (非專利文獻2))等中均已明示此 點)。因此,本發明之有效成分已確認具有真皮之膠原蛋白 產生促進活性,故本發明之有效成分明顯有助於皮膚保 養,更有助於提昇肌膚之彈性與光澤及皺紋之預防或改善。 另,本說明書中,所謂肌膚之彈性與光澤、皺紋等肌 膚或皮膚之狀態之「預防或改善」,包含其狀態之調節、惡 化之延緩、減輕、發作預防、復發預防、抑制等在内之意 義。 故而,一如前述,依據本發明之其它態樣,可提供構 成含有本發明之膠原蛋白產生促進劑之皮膚外用組成物。 皮膚外用組成物宜提供作為醫藥品、準藥物或化粧品,而 更宜提供作為化粧品。 本發明之皮膚外用組成物(尤其化粧品)中,有效成分之 燕窩之酵素分解物之含量(乾物換算)並無特別之限制,依組 201105362 成物形態而不同,但一般作為固態部分而為〇·〇〇卜20重量 百分比,而以0.005〜5重量百分比之範圍為佳,可對應組成 物之形態而適當加以變更。 或’本發明之皮膚外用組成物之燕窩之酵素分解物之 含量(乾物換算)宜為可實現每曰於每平方公分之皮膚上使 用O.Olmg以上,而以0 01mg〜2mg為佳之量。 在本發明之皮膚外用組成物包含有效成分,且無損膠 原蛋白之產生促進效果之限度内,本發明之促進劑可進而 包含其它任意成分。上述之任意成分可為諸如皮膚外用 劑、化粧品慣用之各種成分,可例舉諸如保濕劑、低級醇、 多價醇、醣類、界面活性劑、緩衝劑、乳化劑、安定劑、 增黏劑、抗氧化劑、防腐抗菌劑、螫合劑、pH調整劑、香 料色素、紫外線吸收劑、紫外線散射劑、維他命類、胺 基酸類、抗炎劑、水、胜肽、糖醇類、酵素類、植物萃取 類、抗氧化物質類、滑石粉、黏土、花粉、珍珠粉等。 在此保/愚劑可例舉諸如聚乙二醇、聚丙二醇、丙三 醇丙一醇、山梨糖醇'玻尿酸及硫酸軟骨素等酸性黏多S 201105362 10~100 times of water' and let it stand or stir it under the armpit for 5~48 hours to make it swell and then heat-sterilize at 60~130°c for 5 seconds~30 minutes. The solution is prepared by filtration, and then treated with an enzyme as described above to obtain an enzyme decomposition product of bird's nest. The enzyme decomposition product is the longer the reaction time of the enzyme, and the decomposition of the protein becomes more and more pleasing, and the enzyme decomposition product containing the lower molecular protein can be obtained. In the present invention, the reaction time of the enzyme is 〇 5 to 48 hours, and more preferably 3 to 36 hours, and the optimum degree is about 24 hours, and it is preferred to use an enzyme decomposition product after the enzyme reaction is sufficiently performed. The enzyme decomposition product of the bird's nest thus obtained preferably has a molecular weight of 70 to 200,000, more preferably 70 to 180,000. The molecular weight is 7〇15〇, which is excellent, and 70~120,000 is the best. Further, the average molecular weight (weight average molecular weight) of the enzyme decomposition product of the heat nest is preferably 300 to 1 Torr, 〇〇0, and more preferably 3 〇〇 to 7 〇. The average molecular weight of 300 to 50,000 is very good, 3 to 30,000 is excellent, 3 to 2 is better, and 300 to 15,000 is the best. Skin composition for external use The collagen production promoter of the present invention can be used as it is, but it can also be contained in a composition of various skin materials such as a pure product, and can be used as an additive to produce a collagen production promoting effect. Composition. The resulting composition can be effectively used for skin care, and is more suitable for enhancing the elasticity and luster of the skin and preventing or improving the sensitive texture. The enzyme-decomposing product of the active ingredient of the present invention has a collagen-promoting-promoting activity of human skin dermal fibroblasts (test results of the implementation of 201105362). Therefore, in the present invention, the production-promoting activity of collagen means the action of promoting or improving the production ability of type I collagen of human skin dermal fibroblasts, thereby being intended to increase the amount of collagen protein in the dermis of human skin. By increasing the amount of collagen in the dermis, it is possible to achieve wrinkle improvement and prevention, and to improve the elasticity (elasticity) of the skin and increase the luster of the skin, which is well known to those skilled in the art (such as the aforementioned literature ("for functional cosmetic materials) The development of the "test method" (芋皮玄尔监修), issued in 2〇〇7, CMC published '45th to 47th page (Non-Patent Document 1)) and literature (Akiko Kojima-Yuasa, et al., Fragrance This point is clearly shown in Journal, April, 2009, No. 346 (Vol. 37, No. 4), pp. 103_ (Non-Patent Document 2)) and the like. Therefore, the active ingredient of the present invention has been confirmed to have collagen-promoting activity of the dermis, so that the active ingredient of the present invention contributes significantly to the skin's maintenance and contributes to the prevention or improvement of the elasticity and luster of the skin and wrinkles. In addition, in this manual, the "prevention or improvement" of the skin or skin such as elasticity, luster, and wrinkles of the skin includes adjustment of its state, delay of deterioration, reduction, prevention of seizures, prevention of recurrence, and inhibition. significance. Therefore, as described above, according to another aspect of the present invention, a skin external composition constituting the collagen production promoting agent of the present invention can be provided. The external composition for skin is preferably provided as a pharmaceutical, quasi-drug or cosmetic, and is more preferably provided as a cosmetic. In the skin external composition (especially cosmetic) of the present invention, the content of the enzyme decomposition product (dry matter conversion) of the bird's nest of the active ingredient is not particularly limited, and it differs depending on the form of the composition of the group 201105362, but is generally a solid portion. The content is 20% by weight, and preferably 0.005 to 5% by weight, and can be appropriately changed depending on the form of the composition. Or the content of the enzyme decomposition product of the bird's nest of the external composition for skin of the present invention (dry matter conversion) is preferably such that it can be used in an amount of O.Omg or more per square centimeter of the skin, and preferably 0 01 mg to 2 mg. The accelerator of the present invention may further contain other optional components within the limits of the skin external composition of the present invention containing the active ingredient without impairing the production promoting effect of the collagen. Any of the above components may be various components such as a skin external preparation or a cosmetic, and may, for example, be a moisturizer, a lower alcohol, a polyvalent alcohol, a sugar, a surfactant, a buffer, an emulsifier, a stabilizer, or a tackifier. , antioxidants, antiseptic antibacterial agents, chelating agents, pH adjusters, perfume colors, UV absorbers, UV scattering agents, vitamins, amino acids, anti-inflammatory agents, water, peptides, sugar alcohols, enzymes, plants Extraction, antioxidant substances, talcum powder, clay, pollen, pearl powder, etc. Here, the protective agent can be exemplified by an acid viscosity such as polyethylene glycol, polypropylene glycol, glycerol propanol, sorbitol 'hyaluronic acid, and chondroitin sulfate.
類、寡糖類、 D-山梨醣醇、山梨醇酐、纖維素、澱粉等醣 多醣類及其等之誘導體。 12 201105362 本發明之皮膚外用組成物可採用公知之方法適當調配 包含燕窩酵素分解物之膠原蛋白產生促進劑及上述之任意 成分,而構成洗劑、乳液、乳霜、保濕液、敷面劑、化粧 水、洗面乳、身體乳、身體霜等各種化粧品之慣用產品形 態。本發明之皮膚外用組成物亦可進而提供作為粉底類、 脣膏、眼影、腮紅等彩粧化粧品及防曬用產品、防臭化粧 品等藥用化粧品、洗髮精及潤絲精、造型品等髮粧品、皮 膚清潔劑及入浴劑之浴用化桩品等之使用用途。 依據本發明之其它態樣,—如前述,可提供燕窩之酵 素分解物之使用,目的在製造涵括人類在内之哺乳動物之 皮膚之膠原蛋白產生促進劑。在此,皮膚之膠原蛋白產生 促進宜為皮膚真皮纖維母細胞之〗型膠原蛋白產生之促進。 依據本發明之另一態樣,—如前述,可提供燕寫之酵 素分解物之使用,目的在製造㈣保養用之皮膚外用組成 物。在此’皮膚外用组成物宜使用於肌膚之彈性與光澤之Inducers of sugar polysaccharides such as oligosaccharides, D-sorbitol, sorbitol anhydride, cellulose, starch, and the like. 12 201105362 The skin external composition of the present invention can be appropriately formulated into a collagen production promoter containing a bird's nest enzyme decomposition product and any of the above components by a known method to form a lotion, an emulsion, a cream, a moisturizing liquid, a face lotion, The usual product form of various cosmetics such as lotion, facial cleanser, body lotion, and body cream. The skin external composition of the present invention can further provide hair cosmetic products such as foundations, lipsticks, eye shadows, and blushes, sunscreen products, deodorant cosmetics, and other cosmetic products such as shampoos, moisturizers, and styling products. Uses of skin cleansers and bathing baths for bathing agents, etc. According to another aspect of the present invention, as described above, the use of an enzyme decomposition product of bird's nest can be provided for the purpose of producing a collagen production promoter for the skin of a mammal including humans. Here, the collagen production of the skin promotes the promotion of collagen production which is preferably a type of skin dermal fibroblast. According to another aspect of the present invention, as described above, the use of an enzyme decomposition product of Swallow writing can be provided for the purpose of producing (4) a skin external composition for maintenance. Here, the skin external composition should be used for the elasticity and luster of the skin.
提昇以及敝m改善。又,皮膚外賴成物宜為化 粧品Q 依據本發明之另一態樣,-如前述,可提供皮膚之膠 原蛋白產生促進方法’包含制括人齡内之哺乳動物之 皮膚使用本發明之有效成分之酵素分解物之有效量之程 序。另在此所明「有效量」係指因使用而可於體内之預 定領域内發揮膠原蛋白之產生促進活性之充足量。又,此 之所謂皮狀膠原蛋自之產生促進宜為皮膚真皮纖維母細 胞之I型膠原蛋白產生之促進。Improvement and improvement. Further, the skin-derived product is preferably a cosmetic Q. According to another aspect of the present invention, as described above, a method for promoting collagen production in the skin can be provided, which comprises the use of the skin of a mammal of a human age. The procedure for the effective amount of the enzyme decomposition product of the ingredient. Further, "effective amount" as used herein means a sufficient amount of collagen production promoting activity to be exerted in a predetermined field in the body by use. Further, the so-called skin-like collagen egg produced therefrom promotes the promotion of type I collagen production which is preferably a skin dermal fibroblast.
S 13 201105362 進而,依據本發明之另一其它態樣,可提供一種皮膚 保養方法,包含對涵括人類在内之哺乳動物之皮膚使用本 發明之有效成分之有效量之程序。同樣地,亦可提供包含 對涵括人類在内之哺乳動物之皮膚使用本發明之有效成分 之有效量之程序,而可提昇肌膚之彈性與光澤,並預防或 改善皺紋之方法。另,此之所謂「有效量」係指獲致所欲 之效果所需之充足量。 另,本說明書中,使用「約」及「程度」之用語之值, 包含在達成設定其值之目的之前提下,本業之技術人員所 可容許之值之變動之意義。舉例言之,意指可容許預定值 或範圍之20%以内之變動,而以10%以内為佳,5%以内為 更佳。 實施例 參照以下之例具體說明本發明,但本發明並不受限於 以下之例。 (1)燕窩樣本之調製 (1-1)燕窩酵素分解物1 藉碾磨機磨碎市售之未漂白之燕窩,而製得100篩目大 小(粒徑150μηι以下)之燕窩粉末,再對該燕窩粉末加入約50 倍量(質量)之水而在5°C下使其膨脹20小時後,在121°C下進 行加熱殺菌處理15分鐘。 在製得之處理液冷卻後,調整pH,並對燕窩粉末添加2 質量百分比之量之含蛋白酶酵素(商品名「Pancreatin F」, 天野製藥株式會社出品),而在45°C下進行反應3小時。 14 201105362 將上述酵素反應液調整成PH7.0後,在9(rc下加熱5分 鐘而使酵素不活性化,再加以過據並回收遽液。輯上述 濾液後,予以凍結乾燥而製得「燕窩酵素分解物i」 (1 2)热窩酵素分解物 除酵素反應之時間改為24小時以外,製程均與前迷〇 1 之酵素分解物1相同,而製得「燕窩酵素分解物2」。 〇 (U)热窩熱水萃取物(比較例) 藉碾磨機磨碎市售之未漂白之燕窩(乾燥物),並將其磨 碎物加入l〇〇0ml之蒸餾水中,再藉加熱迴流進行2小時之萃 取處理。加熱迴流後,再予靜置,然後分取上澄液加以過 濾,而回收濾液。殘渣則進而與前述相同,予以加入1〇〇〇如 之蒸餾水中,藉加熱迴流而進行再萃取處理後,過濾固態 物殘渣而加以去除,並回收濾液。所得之濾液(萃取液)則一 同在減壓環境下加以濃縮,所得之濃縮物則予以凍結乾 燥,而製得黃白色之「燕窩熱水萃取物」(比較例)。 (2)燕窩樣本之分析 製得之各燕窩樣本(燕窩酵素分解物1、燕窩酵素分解 物2及燕窩熱水萃取物)中之涎酸量與蛋白質量已測定如 下。 延酸之量係在各試料之酸性水解後藉高速液體層析法 而測定游離N-乙醯神經氨酸所得。 蛋白質之量則使用基於Brad f〇rd法之Bio Rad公司之 Protein Assay Kit 而加以測定。 結果則如第1及第2圖所示。Further, according to still another aspect of the present invention, there is provided a skin care method comprising the step of using an effective amount of the active ingredient of the present invention on the skin of a mammal including a human. Similarly, a method of using an effective amount of the active ingredient of the present invention for the skin of a mammal including humans to enhance the elasticity and luster of the skin and prevent or improve wrinkles can be provided. In addition, the so-called "effective amount" refers to the sufficient amount required to achieve the desired effect. In addition, the values of the terms "approximately" and "degree" are used in this specification to include the meaning of the changes that can be tolerated by the skilled person in the industry before the purpose of setting the value. For example, it means that the change within 20% of the predetermined value or range is allowed, and preferably within 10%, and preferably within 5%. EXAMPLES The present invention will be specifically described with reference to the following examples, but the present invention is not limited by the following examples. (1) Preparation of bird's nest sample (1-1) Bird's nest enzyme decomposition product 1 The unwashed bird's nest is ground by a mill to obtain a bird's nest powder of 100 mesh size (particle size below 150 μηι), and then The bird's nest powder was added to about 50 times by mass of water and expanded at 5 ° C for 20 hours, and then heat-sterilized at 121 ° C for 15 minutes. After the obtained treatment liquid was cooled, the pH was adjusted, and 2% by mass of the protease-containing enzyme (trade name "Pancreatin F", manufactured by Amano Pharmaceutical Co., Ltd.) was added to the bird's nest powder, and the reaction was carried out at 45 ° C. hour. 14 201105362 After adjusting the above enzyme reaction solution to pH 7.0, the enzyme is inactivated by heating at 9 (rc for 5 minutes), and the sputum is recovered. The filtrate is collected and freeze-dried to obtain " Bird's Nest Enzyme Decomposition i" (1 2) The heat-decomposing enzyme decomposition product was changed to the same time as the enzyme decomposition product 1 of the former lost one, except that the enzyme reaction time was changed to 24 hours, and the "bird's nest enzyme decomposition product 2" was obtained. 〇(U) hot water hot water extract (Comparative example) The commercially available unbleached bird's nest (dry matter) is ground by a mill, and the ground material is added to 10 ml of distilled water, and then borrowed. The mixture is heated and refluxed for 2 hours, and after heating and refluxing, it is allowed to stand still, and then the supernatant liquid is separated and filtered, and the filtrate is recovered. The residue is further added to distilled water as in the above. After re-extraction treatment by heating under reflux, the solid residue is filtered and removed, and the filtrate is recovered. The obtained filtrate (extract solution) is concentrated together under reduced pressure, and the resulting concentrate is freeze-dried to obtain a concentrate. Yellow white "Swallow's Nest Hot Water Extract" (Comparative Example). (2) The amount of tannic acid in each bird's nest sample (skin nest enzyme decomposition product 1, bird's nest enzyme decomposition product 2 and bird's nest hot water extract) obtained by analysis of bird's nest samples The protein quality has been determined as follows. The amount of the acid is determined by high-speed liquid chromatography after the acidic hydrolysis of each sample, and the amount of protein is determined by the Brad f〇rd method. The company's Protein Assay Kit was measured. The results are shown in Figures 1 and 2.
S 15 201105362 其次’為測定燕窩酵素分解物1及燕窩酵素分解物2所 含之蛋白質之分子量及其分布,而在以下條件下,進行了 GPC之分析。 [HPLC之測定條件]S 15 201105362 Next, in order to measure the molecular weight and distribution of the protein contained in the bird's nest enzyme decomposition product 1 and the bird's nest enzyme decomposition product 2, GPC analysis was carried out under the following conditions. [HPLC measurement conditions]
•管柱:ShodexAsahipakGS320HQ((/>7.6x300mm) •管柱溫度:35°C •移動相:50mM CH3COONH3 pH6.7 •流迷:〇.4ml/min • s式料注入量:1 〇 μΐ •偵測器:紫外分光偵測器(UV220nm之測定) [使用之分子量標示器] • BSAMW66,000 ’甘胺醯甘胺酸(Gly-gly)MW132.1 •三胜肽(Gly-gly-gly)MW189.17 .L-天冬胺酸(L-Asparaginic acid) MW133.1 .DL-天冬胺酸(DL-Asparaginic acid) MW150.1 N_ 乙酿神經氨酸(N-Acetylneuraminic acid) MW309.3 測定結果則如第3及第4圖所示。 已就燕窩酵素分解物所含之蛋白質之分子量,使用西 方墨點法而加以評價。具體而言,係先依循前述之測定法 而確認燕窩酵素分解物中之蛋白質之量。就燕窩酵素分解 物來源之蛋白質(100呢)使用SDS-PAGE而藉10〜150/〇之聚丙 酿胺加以分離。將已分散於膠體中之蛋白質轉印至 Immimoblot PVDF 膜(聚偏二氟乙烯(p〇iyvinyHdene 16 201105362 difluoride),取自Bio-Lad Lab公司)。再於包含3%之脫脂乳 與0.1%之Tween-20之PBS中對PVDF膜進行阻隔1小時,再 加以進行視覺上之判定。 另,評價蛋白質之分子量時,亦已參考公知文獻(Gu〇, C. T., Suzuki Y., et al., Antiviral Res. 70, pp. 140-146 (2006)) 所揭露之資料。 由上述結果可知’燕窩酵素分解物中所含之蛋白質分 子量存在約70〜120,000程度(另,視評價條件之不同,上限 值可達200,000程度)之範圍内。 進而,由所得結果可知,燕窩酵素分解物丨之蛋白質之 平均分子量約為55,000。又,可知燕窩酵素分解物2之蛋白 質之平均分子量約為33,000。 (3)膠原蛋白之產生促進能力之測定 已使用含有0.5%幼牛血清(FBS)之DMEM培養基(取自 SIGMA)於96孔板上按2χΐ〇4ceiis之細胞密度種植正常人類 皮膚真皮纖維母細胞(KF_4l〇9,取自KURABO公司)。種植 24小時後’更換為内含含有預定濃度之各燕窩樣本之 0.5°/〇FBS之DMEM培養基,再進行培養24小時。 且,已使用左旋C磷酸鎂(VC-PMg)作為正控制(P. C.)。 培養後,回收培養基上澄液,並藉ELISA法測定上澄 液中之I型膠原蛋白量。另,ELISA法係使用Anti-Human Collagen Type 1抗體(Rabit)作為一次抗體,並使用 Hist〇fine PO(Rabit)作為二次抗體,進而發色劑則使用2,2ι_連氮基_雙 -(3-乙基苯並二氫噻唑啉_6_磺酸xABTS),而藉微量盤判讀 5 17 201105362 儀測定了 405nm之吸光度。 培養基中之I型膠原蛋白量已藉培養基中之I型膠原蛋 白量除以全部細胞之蛋白質量而算出各單位蛋白質量之I 型膠原蛋白產生量。 結果則如第5圖所示。 由結果可知,燕窩之酵素分解物可積極使人類皮膚真 皮纖維母細胞之I型膠原蛋白增生。 C圖式簡單說明3 第1圖係顯示燕窩樣本中之涎酸量之測定結果之圖表。 第2圖係顯示燕窩樣本中之蛋白質量之測定結果之圖 表。 第3圖係顯示採GPC-HPLC之燕窩酵素分解物1之測定 結果之圖表。 第4圖係顯示採GPC-HPLC之燕窩酵素分解物2之測定 結果之圖表。 第5圖係顯示膠原蛋白之產生促進能力之測定結果之 圖表。 【主要元件符號說明】 (無) 18• Column: ShodexAsahipakGS320HQ ((/>7.6x300mm) • Column temperature: 35°C • Mobile phase: 50mM CH3COONH3 pH6.7 • Flow fan: 〇.4ml/min • s type material injection amount: 1 〇μΐ • Detector: UV spectrometer (measured by UV220nm) [Molecular weight marker used] • BSAMW66,000 'Gly-gly MW132.1 • Three peptides (Gly-gly-gly) ) MW189.17 .L-Asparaginic acid MW133.1 .DL-Asparaginic acid MW150.1 N_ N-Acetylneuraminic acid MW309. 3 The results are shown in Figures 3 and 4. The molecular weight of the protein contained in the bird's nest enzyme decomposition product has been evaluated using the Western blot method. Specifically, the bird's nest is confirmed by the above-mentioned assay. The amount of protein in the enzyme decomposition product. The protein derived from the enzyme decomposition of bird's nest enzyme (100) is separated by 10 to 150/〇 polyacrylamide using SDS-PAGE. The protein dispersed in the colloid is transferred to the protein. Immimoblot PVDF membrane (polyvinylidene fluoride (p〇iyvinyHdene 16 201105362 difluoride), taken from Bio-Lad Lab Company). The PVDF membrane was blocked for 1 hour in PBS containing 3% skim milk and 0.1% Tween-20, and then visually judged. In addition, when evaluating the molecular weight of protein, reference has also been made to the public literature. (Gu〇, CT, Suzuki Y., et al., Antiviral Res. 70, pp. 140-146 (2006)). The above results show that the molecular weight of the protein contained in the bird's nest enzyme decomposition is about The range of 70 to 120,000 (in addition, depending on the evaluation conditions, the upper limit can reach 200,000). Further, from the results, it can be seen that the average molecular weight of the protein of the bird's nest enzyme decomposition product is about 55,000. The average molecular weight of the protein of the enzyme decomposition product 2 is about 33,000. (3) Determination of the ability to promote the production of collagen The DMEM medium (taken from SIGMA) containing 0.5% young bovine serum (FBS) has been used in a 96-well plate. The cell density of 〇4ceiis was implanted in normal human skin dermal fibroblasts (KF_4l〇9, taken from KURABO). After 24 hours of planting, 'replaced with D of 0.5°/〇FBS containing samples of each bird's nest containing a predetermined concentration. The MEM medium was further cultured for 24 hours. Moreover, L-C magnesium phosphate (VC-PMg) has been used as a positive control (P. C.). After the culture, the medium was recovered and the amount of type I collagen in the supernatant was determined by ELISA. In addition, the ELISA method uses Anti-Human Collagen Type 1 antibody (Rabit) as a primary antibody, and Hist〇fine PO (Rabit) as a secondary antibody, and then a color former using 2,2ι_nitrogen-double- (3-ethylbenzodihydrothiazoline_6_sulfonic acid xABTS), and absorbance at 405 nm was determined by microplate interpretation 5 17 201105362. The amount of type I collagen in the medium was calculated by dividing the amount of type I collagen in the medium by the amount of protein in all the cells to calculate the amount of type I collagen produced per unit amount of protein. The result is shown in Figure 5. It can be seen from the results that the enzyme decomposition product of bird's nest can actively proliferate type I collagen of human skin dermal fibroblasts. Brief Description of C Schematic 3 Figure 1 shows a graph showing the results of the determination of the amount of tannic acid in a bird's nest sample. Figure 2 is a graph showing the results of the determination of the amount of protein in the bird's nest samples. Fig. 3 is a graph showing the results of measurement of the bird's nest enzyme decomposition product 1 by GPC-HPLC. Fig. 4 is a graph showing the results of measurement of the bird's nest enzyme decomposition product 2 by GPC-HPLC. Fig. 5 is a graph showing the results of measurement of the ability to promote collagen production. [Main component symbol description] (none) 18
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JP4976629B2 (en) * | 2001-09-27 | 2012-07-18 | コンビ株式会社 | Skin promotion agent |
JP4559089B2 (en) * | 2004-01-23 | 2010-10-06 | コンビ株式会社 | Virus capture spray and virus capture filter |
GB0411387D0 (en) * | 2004-05-21 | 2004-06-23 | Chiron Srl | Analysis of saccharide length |
CN100531708C (en) * | 2007-06-19 | 2009-08-26 | 浙江大学 | Process for preparing edible bird's nest extraction used for cosmetics |
US20100143535A1 (en) * | 2007-07-12 | 2010-06-10 | Hidemasa Motoshima | Method for producing composition containing sialic acid compound |
JP2009073806A (en) * | 2007-08-27 | 2009-04-09 | Iriya Cosmetics Co Ltd | Insulin-like growth factor-1 secretagogue |
CN101606900B (en) * | 2008-06-17 | 2012-12-05 | 深圳绵俪日用化工有限公司 | Whitening facial mask containing cubilose extracting solution |
JP5676077B2 (en) * | 2008-12-22 | 2015-02-25 | コンビ株式会社 | Bone strength improver |
-
2009
- 2009-06-23 JP JP2009148754A patent/JP5815202B2/en active Active
-
2010
- 2010-06-21 CN CN2010800282277A patent/CN102458357A/en active Pending
- 2010-06-21 WO PCT/JP2010/060459 patent/WO2010150737A1/en active Application Filing
- 2010-06-21 CN CN201610983140.4A patent/CN107049907A/en active Pending
- 2010-06-21 KR KR1020127001655A patent/KR20120106703A/en not_active Application Discontinuation
- 2010-06-23 TW TW099120421A patent/TW201105362A/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
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CN107049907A (en) | 2017-08-18 |
JP5815202B2 (en) | 2015-11-17 |
KR20120106703A (en) | 2012-09-26 |
TWI559939B (en) | 2016-12-01 |
JP2011006331A (en) | 2011-01-13 |
WO2010150737A1 (en) | 2010-12-29 |
CN102458357A (en) | 2012-05-16 |
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