TW201105362A - Collagen production enhancer, and external composition for skin and cosmetic each comprising same - Google Patents

Abstract

Disclosed is a collagen production enhancer, which comprises an enzymatically decomposed product of swallow's nest as an active ingredient. Specifically disclosed are: an excellent type-I collagen production enhancer which can be produced with high efficiency and is highly safe; and an external preparation and a cosmetic, each of which comprises the type-I collagen production enhancer.

Description

201105362 VI. INSTRUCTIONS: c. Inventives in the field of technology 3 Inter-references to related applications This application is based on the patent application No. 2009-148754, which was first filed in Japan (application date: June 2009) On the basis of the 23rd, and claiming the benefits of its priority, and its overall disclosure is incorporated by reference. . BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a collagen production promoter which uses an enzyme decomposition product of bird's nest as an effective component. More specifically, the present invention relates to a collagen production promoter which promotes the production of type I collagen of skin dermal fibroblasts. Further, the present invention relates to a skin external composition and a cosmetic comprising an enzyme decomposition product of bird's nest containing the above-mentioned active ingredient. Background Art Skin problems such as dry skin, wrinkles, sagging skin, and poor elasticity are particularly valued and cared for by women. These skin problems are usually thought to be caused by environmental factors, ageing factors such as ageing, and even psychological factors such as stress. In particular, environmental factors that have adversely affected the skin caused by the increase in ultraviolet rays and the drying of the living environment in recent years have been gradually increasing. The skin tissue consists of epidermis, dermis, and subcutaneous tissue. The dermis contains a lot of collagen and hyaluronic acid, which is closely related to the water retention and elasticity of the skin.

S 3 201105362 Related. Collagen and hyaluronic acid are known to be produced by dermal fibroblasts present in the dermis. Collagen is said to account for about 30% of the protein in the body, 70% of which is found in the dermis. Collagen in the dermis contains collagen I and collagen type III, but mainly type I collagen. In the past, in order to improve the skin's elasticity, luster, and wrinkles, it is common to use a collagen-added cosmetic for external use and a method of directly supplementing the skin with collagen. However, when collagen is added by external means, it is difficult to increase the collagen of the skin dermis, so it is difficult to increase the elasticity and gloss of the skin and prevent wrinkles. If the skin is exposed to light containing ultraviolet light, it is known that most of the collagen in the extracellular matrix of the dermis will be significantly reduced and the fiber bundles will be irregularly arranged. Therefore, the increase in the amount of collagen in the dermis contributes to the improvement and prevention of wrinkles (Document 1 "Experimental method for the development of functional cosmetic materials" (Supervised by Suo Xuan Xuan), issued in 2007, published by CMC, No. 45 ~47 pages). In addition, it is also revealed that ultraviolet light (UVB) irradiation will greatly reduce the collagen synthesis of dermal fibroblasts. Among them, the collagen-type synthesis promoter is exemplified by a vitamin C inducer, a whey component, and the like. Another 'Document 2 (Akiko Kojima-Yuasa, et al., Fragrance Journal, April, 2009, No. 346 (Vol. 37, No. 4), pp. l〇3-)t UJ reveals collagen production ability The reduction of the inhibition contributes to the improvement of Q〇L, and the decrease in the amount of collagen in the skin is caused by wrinkles and slacks. The type I collagen production promoter has hitherto been reported to have a large number of reports on the use of specific plant-derived components (such as Japanese Patent Laid-Open No. Hei 2, No. 5,984, No. 4, 2011, 052, and WO2004/085429). The swallow's nest swiftlets form a nest of filaments in their own saliva, and it is known that their constituents contain a large amount of cool protein' and are almost free of lipids. Yan wrote that it is used in Chinese cuisine as a raw material. It is also a raw material for traditional Chinese medicine. In recent years, health foods such as bird's nest have been developed, and applications for external use such as external preparations and cosmetics are still less well known. A cosmetic agent for oral use using an enzyme decomposition product of bird's nest has been disclosed in Japanese Laid-Open Patent Publication No. 2003-95961. However, only oral use is disclosed therein, and no disclosure or suggestion is made as to the possibility of use for external use. Moreover, the effect is that the moisturizing effect of the skin and the realization of the delicate skin of the skin are not disclosed in the ability to promote the growth and elasticity of the skin and to improve the production of type I collagen of wrinkles. In WO 99/022709, a cosmetic comprising an aqueous solvent extract of bird's nest, in particular a hot water extract, has been disclosed. The cosmetic can be used for skin care purposes and is used for external use. Further, it is also revealed that the aqueous extract of bird's nest has a collagen synthesis promoting effect on skin cells. However, the extracts used in the bird's nest are extracted by hot water extraction to remove the solid portion, and the bird's nest enzyme decomposition products which are directly used for the enzyme treatment of the bird's nest are obviously different. Further, regarding the effect, it is considered that there is still room for improvement in consideration of the present inventors. [Immediately, the present invention has found that the enzyme decomposition product of bird's nest has an excellent production-promoting effect of type I collagen of skin dermal fibroblasts. Its effect is better

S 201105362 The effect of the hot water extract of bird's nest is more pronounced, and the above effect of the high enzyme decomposition product is more obvious. Ben:: Discovery is the basis. In the month of i Xuanqi, the purpose is to provide efficient production and safety: = (4) protein production promotion (4) for use in addition to the former:: collagen production promoter - enzyme decomposition products according to this month - compared In the best case, the aforementioned enzyme decomposes (4) a decomposition product of the protease. More preferably, the molecular weight of the aforementioned enzyme decomposition product is 70 to 200,000 in accordance with the present invention. According to a preferred embodiment of the present invention, the average molecular weight of the aforementioned enzyme decomposition product is 300 to 15,00 Å. According to a preferred embodiment of the present invention, the collagen production promoting agent of the present invention can promote the production of protoproteins of dermal fibroblasts. According to other aspects of the present invention, there is provided a substance comprising the gel eyebrow of the present invention. Residue should be used for skin care. It is more suitable for improving the elasticity and luster of the skin to prevent or improve wrinkles. U's better sample of the skin of the invention. /Wind According to other aspects of this month, the use of the enzyme decomposition of bird's nest can be provided to produce a collagen production promoter for the skin of animal animals including humans. According to another aspect of the present invention, there is provided a use of an enzyme decomposition product of bird's nest, which is useful for producing a skin external composition for skin care. Further, according to another aspect of the present invention, there is provided a method for promoting the production of a collagen protein of the skin comprising: an effective amount of an enzyme decomposition product of the present invention, which is applied to a skin of a mammal which includes humans. A skin care method according to another aspect of the present invention may comprise: applying an effective amount of the present intensive ingredient to the skin of a milking animal including humans. Similarly, a method can be provided comprising: preparing an effective active ingredient of the present invention on the skin of a milk-producing animal including humans; it can enhance the elasticity and luster of the skin, and prevent or improve the crepe . According to the present invention, the use of the enzyme decomposition product of Yangao as an active ingredient can more significantly promote the production of the primordial protein of human skin dermal fibroblasts than in the past. X, based on the collagen production promoting ability of the above dermal fibroblasts, and the use of the enzyme decomposition product for external use or cosmetic use of the skin can enhance the elasticity (elasticity) of the skin and increase the skin's luster, thereby preventing or reducing wrinkles. . Further, it is possible to promote the proliferation of collagen in the state where the collagen synthesis is reduced after the sun exposure, thereby enhancing the elasticity and luster of the skin and improving the enemy pattern. Thus, according to the present invention, the improvement of the consumer's Q〇L can be achieved. X, the enzyme decomposition product used is: It is also known as the bird's nest of the foodstuff, so the safety is high and the consumer acceptance is also high. Further, the enzyme decomposition product can be obtained by directly performing enzyme treatment on the bird's nest, so that it can be efficiently manufactured and the manufacturing cost can be reduced. Simple illustration

S 201105362 The i® series shows a graph showing the results of the determination of the amount of tannic acid in the bird's nest samples. Figure 2 is a graph showing the results of the determination of the amount of protein in the bird's nest samples. Fig. 3 is a graph showing the results of measurement of the bird's nest enzyme decomposition product 1 of GP C - ΗP L C . Fig. 4 is a graph showing the results of measurement of the bird's nest enzyme decomposition product 2 by GPC-HPLC. Fig. 5 is a graph showing the results of measurement of the ability to promote collagen production. DETAILED DESCRIPTION OF THE INVENTION Active ingredient The collagen production promoter of the present invention, as described above, is an enzyme decomposition product of bird's nest as an active ingredient. The raw material of the enzyme decomposition product, the swiftlet swallows form a nest of their saliva and form a nest. The ingredients contain a large amount of glycoprotein and contain almost no lipids. Here, the swiftlet refers to the swallows under the genus Collocalia of Apodidae, and may be exemplified by ApAdidae Collocalia francica, Moss Nest swiftlet ( Apodidae Collocalia salanga), Apodidae Collocalia fuciphaga, Apodidae Collocalia inexpectat, Apodidae Collocalia vestita, Apodidae Collocalia esculent, Apodidae Collocalia maximus and the like. 201105362 In the present invention, a commercially available product can be used for the bird's nest. Generally, the bird's nest that is commercially available from the cleaning of only the dirt such as swallows and feces, to the collection of the waste of the bird's nest, and the white and cleansing of the bird's nest, includes a variety of types, but the bird's nest used in the present invention is It is advisable to use bird's nest that has not been cleaned and bleached prior to pretreatment. "The enzyme decomposition product of the nest is known to be decomposed by the enzyme reaction of the bird's nest. It can be obtained by decomposing swallowing or its treatment with a protease or a compound enzyme containing a protease. Here, the decomposition treatment is used. The enzyme is preferably a protease, and it is better to use pancreatic enzyme. The enzyme decomposition product of bird's nest can usually be heat-sterilized and then fermented in water. (4) The conditions of treatment can be adjusted according to the conventional heat sterilization conditions (4). The yeast (4) can also be appropriately set according to the type of enzyme used and the state of the bird's nest. More specifically, the enzyme decomposition of bird's nest can be pasteed in the following way. For example, the ground is broken. The bird's nest is added with 1质量~5〇 times the mass of the water to make it swell and then heated at 60~UGtT_treatment for 5 seconds to 3 minutes. Then 'add the right amount of enzyme directly, and the best yang in the enzyme, The enzyme reaction is carried out at the optimum temperature for 5 to 48 hours. After the end of the reaction, the granzyme is inactivated by heat treatment, and the insoluble matter is removed by the solution W, and the enzyme decomposition product of the bird's nest can be obtained. The above-mentioned enzyme decomposition products can also be processed as needed. The above-mentioned materials can also be subjected to pH adjustment, decolorization, deodorization, etc. In other cases, when the enzyme is decomposed by the treatment of the Swallow, the particles are granulated. Below the 2nd surface, 150 is better than the grated swallow to write its quality: 9

S 201105362 10~100 times of water' and let it stand or stir it under the armpit for 5~48 hours to make it swell and then heat-sterilize at 60~130°c for 5 seconds~30 minutes. The solution is prepared by filtration, and then treated with an enzyme as described above to obtain an enzyme decomposition product of bird's nest. The enzyme decomposition product is the longer the reaction time of the enzyme, and the decomposition of the protein becomes more and more pleasing, and the enzyme decomposition product containing the lower molecular protein can be obtained. In the present invention, the reaction time of the enzyme is 〇 5 to 48 hours, and more preferably 3 to 36 hours, and the optimum degree is about 24 hours, and it is preferred to use an enzyme decomposition product after the enzyme reaction is sufficiently performed. The enzyme decomposition product of the bird's nest thus obtained preferably has a molecular weight of 70 to 200,000, more preferably 70 to 180,000. The molecular weight is 7〇15〇, which is excellent, and 70~120,000 is the best. Further, the average molecular weight (weight average molecular weight) of the enzyme decomposition product of the heat nest is preferably 300 to 1 Torr, 〇〇0, and more preferably 3 〇〇 to 7 〇. The average molecular weight of 300 to 50,000 is very good, 3 to 30,000 is excellent, 3 to 2 is better, and 300 to 15,000 is the best. Skin composition for external use The collagen production promoter of the present invention can be used as it is, but it can also be contained in a composition of various skin materials such as a pure product, and can be used as an additive to produce a collagen production promoting effect. Composition. The resulting composition can be effectively used for skin care, and is more suitable for enhancing the elasticity and luster of the skin and preventing or improving the sensitive texture. The enzyme-decomposing product of the active ingredient of the present invention has a collagen-promoting-promoting activity of human skin dermal fibroblasts (test results of the implementation of 201105362). Therefore, in the present invention, the production-promoting activity of collagen means the action of promoting or improving the production ability of type I collagen of human skin dermal fibroblasts, thereby being intended to increase the amount of collagen protein in the dermis of human skin. By increasing the amount of collagen in the dermis, it is possible to achieve wrinkle improvement and prevention, and to improve the elasticity (elasticity) of the skin and increase the luster of the skin, which is well known to those skilled in the art (such as the aforementioned literature ("for functional cosmetic materials) The development of the "test method" (芋皮玄尔监修), issued in 2〇〇7, CMC published '45th to 47th page (Non-Patent Document 1)) and literature (Akiko Kojima-Yuasa, et al., Fragrance This point is clearly shown in Journal, April, 2009, No. 346 (Vol. 37, No. 4), pp. 103_ (Non-Patent Document 2)) and the like. Therefore, the active ingredient of the present invention has been confirmed to have collagen-promoting activity of the dermis, so that the active ingredient of the present invention contributes significantly to the skin's maintenance and contributes to the prevention or improvement of the elasticity and luster of the skin and wrinkles. In addition, in this manual, the "prevention or improvement" of the skin or skin such as elasticity, luster, and wrinkles of the skin includes adjustment of its state, delay of deterioration, reduction, prevention of seizures, prevention of recurrence, and inhibition. significance. Therefore, as described above, according to another aspect of the present invention, a skin external composition constituting the collagen production promoting agent of the present invention can be provided. The external composition for skin is preferably provided as a pharmaceutical, quasi-drug or cosmetic, and is more preferably provided as a cosmetic. In the skin external composition (especially cosmetic) of the present invention, the content of the enzyme decomposition product (dry matter conversion) of the bird's nest of the active ingredient is not particularly limited, and it differs depending on the form of the composition of the group 201105362, but is generally a solid portion. The content is 20% by weight, and preferably 0.005 to 5% by weight, and can be appropriately changed depending on the form of the composition. Or the content of the enzyme decomposition product of the bird's nest of the external composition for skin of the present invention (dry matter conversion) is preferably such that it can be used in an amount of O.Omg or more per square centimeter of the skin, and preferably 0 01 mg to 2 mg. The accelerator of the present invention may further contain other optional components within the limits of the skin external composition of the present invention containing the active ingredient without impairing the production promoting effect of the collagen. Any of the above components may be various components such as a skin external preparation or a cosmetic, and may, for example, be a moisturizer, a lower alcohol, a polyvalent alcohol, a sugar, a surfactant, a buffer, an emulsifier, a stabilizer, or a tackifier. , antioxidants, antiseptic antibacterial agents, chelating agents, pH adjusters, perfume colors, UV absorbers, UV scattering agents, vitamins, amino acids, anti-inflammatory agents, water, peptides, sugar alcohols, enzymes, plants Extraction, antioxidant substances, talcum powder, clay, pollen, pearl powder, etc. Here, the protective agent can be exemplified by an acid viscosity such as polyethylene glycol, polypropylene glycol, glycerol propanol, sorbitol 'hyaluronic acid, and chondroitin sulfate.

Inducers of sugar polysaccharides such as oligosaccharides, D-sorbitol, sorbitol anhydride, cellulose, starch, and the like. 12 201105362 The skin external composition of the present invention can be appropriately formulated into a collagen production promoter containing a bird's nest enzyme decomposition product and any of the above components by a known method to form a lotion, an emulsion, a cream, a moisturizing liquid, a face lotion, The usual product form of various cosmetics such as lotion, facial cleanser, body lotion, and body cream. The skin external composition of the present invention can further provide hair cosmetic products such as foundations, lipsticks, eye shadows, and blushes, sunscreen products, deodorant cosmetics, and other cosmetic products such as shampoos, moisturizers, and styling products. Uses of skin cleansers and bathing baths for bathing agents, etc. According to another aspect of the present invention, as described above, the use of an enzyme decomposition product of bird's nest can be provided for the purpose of producing a collagen production promoter for the skin of a mammal including humans. Here, the collagen production of the skin promotes the promotion of collagen production which is preferably a type of skin dermal fibroblast. According to another aspect of the present invention, as described above, the use of an enzyme decomposition product of Swallow writing can be provided for the purpose of producing (4) a skin external composition for maintenance. Here, the skin external composition should be used for the elasticity and luster of the skin.

Improvement and improvement. Further, the skin-derived product is preferably a cosmetic Q. According to another aspect of the present invention, as described above, a method for promoting collagen production in the skin can be provided, which comprises the use of the skin of a mammal of a human age. The procedure for the effective amount of the enzyme decomposition product of the ingredient. Further, "effective amount" as used herein means a sufficient amount of collagen production promoting activity to be exerted in a predetermined field in the body by use. Further, the so-called skin-like collagen egg produced therefrom promotes the promotion of type I collagen production which is preferably a skin dermal fibroblast.

Further, according to still another aspect of the present invention, there is provided a skin care method comprising the step of using an effective amount of the active ingredient of the present invention on the skin of a mammal including a human. Similarly, a method of using an effective amount of the active ingredient of the present invention for the skin of a mammal including humans to enhance the elasticity and luster of the skin and prevent or improve wrinkles can be provided. In addition, the so-called "effective amount" refers to the sufficient amount required to achieve the desired effect. In addition, the values of the terms "approximately" and "degree" are used in this specification to include the meaning of the changes that can be tolerated by the skilled person in the industry before the purpose of setting the value. For example, it means that the change within 20% of the predetermined value or range is allowed, and preferably within 10%, and preferably within 5%. EXAMPLES The present invention will be specifically described with reference to the following examples, but the present invention is not limited by the following examples. (1) Preparation of bird's nest sample (1-1) Bird's nest enzyme decomposition product 1 The unwashed bird's nest is ground by a mill to obtain a bird's nest powder of 100 mesh size (particle size below 150 μηι), and then The bird's nest powder was added to about 50 times by mass of water and expanded at 5 ° C for 20 hours, and then heat-sterilized at 121 ° C for 15 minutes. After the obtained treatment liquid was cooled, the pH was adjusted, and 2% by mass of the protease-containing enzyme (trade name "Pancreatin F", manufactured by Amano Pharmaceutical Co., Ltd.) was added to the bird's nest powder, and the reaction was carried out at 45 ° C. hour. 14 201105362 After adjusting the above enzyme reaction solution to pH 7.0, the enzyme is inactivated by heating at 9 (rc for 5 minutes), and the sputum is recovered. The filtrate is collected and freeze-dried to obtain " Bird's Nest Enzyme Decomposition i" (1 2) The heat-decomposing enzyme decomposition product was changed to the same time as the enzyme decomposition product 1 of the former lost one, except that the enzyme reaction time was changed to 24 hours, and the "bird's nest enzyme decomposition product 2" was obtained. 〇(U) hot water hot water extract (Comparative example) The commercially available unbleached bird's nest (dry matter) is ground by a mill, and the ground material is added to 10 ml of distilled water, and then borrowed. The mixture is heated and refluxed for 2 hours, and after heating and refluxing, it is allowed to stand still, and then the supernatant liquid is separated and filtered, and the filtrate is recovered. The residue is further added to distilled water as in the above. After re-extraction treatment by heating under reflux, the solid residue is filtered and removed, and the filtrate is recovered. The obtained filtrate (extract solution) is concentrated together under reduced pressure, and the resulting concentrate is freeze-dried to obtain a concentrate. Yellow white "Swallow's Nest Hot Water Extract" (Comparative Example). (2) The amount of tannic acid in each bird's nest sample (skin nest enzyme decomposition product 1, bird's nest enzyme decomposition product 2 and bird's nest hot water extract) obtained by analysis of bird's nest samples The protein quality has been determined as follows. The amount of the acid is determined by high-speed liquid chromatography after the acidic hydrolysis of each sample, and the amount of protein is determined by the Brad f〇rd method. The company's Protein Assay Kit was measured. The results are shown in Figures 1 and 2.

S 15 201105362 Next, in order to measure the molecular weight and distribution of the protein contained in the bird's nest enzyme decomposition product 1 and the bird's nest enzyme decomposition product 2, GPC analysis was carried out under the following conditions. [HPLC measurement conditions]

• Column: ShodexAsahipakGS320HQ ((/>7.6x300mm) • Column temperature: 35°C • Mobile phase: 50mM CH3COONH3 pH6.7 • Flow fan: 〇.4ml/min • s type material injection amount: 1 〇μΐ • Detector: UV spectrometer (measured by UV220nm) [Molecular weight marker used] • BSAMW66,000 'Gly-gly MW132.1 • Three peptides (Gly-gly-gly) ) MW189.17 .L-Asparaginic acid MW133.1 .DL-Asparaginic acid MW150.1 N_ N-Acetylneuraminic acid MW309. 3 The results are shown in Figures 3 and 4. The molecular weight of the protein contained in the bird's nest enzyme decomposition product has been evaluated using the Western blot method. Specifically, the bird's nest is confirmed by the above-mentioned assay. The amount of protein in the enzyme decomposition product. The protein derived from the enzyme decomposition of bird's nest enzyme (100) is separated by 10 to 150/〇 polyacrylamide using SDS-PAGE. The protein dispersed in the colloid is transferred to the protein. Immimoblot PVDF membrane (polyvinylidene fluoride (p〇iyvinyHdene 16 201105362 difluoride), taken from Bio-Lad Lab Company). The PVDF membrane was blocked for 1 hour in PBS containing 3% skim milk and 0.1% Tween-20, and then visually judged. In addition, when evaluating the molecular weight of protein, reference has also been made to the public literature. (Gu〇, CT, Suzuki Y., et al., Antiviral Res. 70, pp. 140-146 (2006)). The above results show that the molecular weight of the protein contained in the bird's nest enzyme decomposition is about The range of 70 to 120,000 (in addition, depending on the evaluation conditions, the upper limit can reach 200,000). Further, from the results, it can be seen that the average molecular weight of the protein of the bird's nest enzyme decomposition product is about 55,000. The average molecular weight of the protein of the enzyme decomposition product 2 is about 33,000. (3) Determination of the ability to promote the production of collagen The DMEM medium (taken from SIGMA) containing 0.5% young bovine serum (FBS) has been used in a 96-well plate. The cell density of 〇4ceiis was implanted in normal human skin dermal fibroblasts (KF_4l〇9, taken from KURABO). After 24 hours of planting, 'replaced with D of 0.5°/〇FBS containing samples of each bird's nest containing a predetermined concentration. The MEM medium was further cultured for 24 hours. Moreover, L-C magnesium phosphate (VC-PMg) has been used as a positive control (P. C.). After the culture, the medium was recovered and the amount of type I collagen in the supernatant was determined by ELISA. In addition, the ELISA method uses Anti-Human Collagen Type 1 antibody (Rabit) as a primary antibody, and Hist〇fine PO (Rabit) as a secondary antibody, and then a color former using 2,2ι_nitrogen-double- (3-ethylbenzodihydrothiazoline_6_sulfonic acid xABTS), and absorbance at 405 nm was determined by microplate interpretation 5 17 201105362. The amount of type I collagen in the medium was calculated by dividing the amount of type I collagen in the medium by the amount of protein in all the cells to calculate the amount of type I collagen produced per unit amount of protein. The result is shown in Figure 5. It can be seen from the results that the enzyme decomposition product of bird's nest can actively proliferate type I collagen of human skin dermal fibroblasts. Brief Description of C Schematic 3 Figure 1 shows a graph showing the results of the determination of the amount of tannic acid in a bird's nest sample. Figure 2 is a graph showing the results of the determination of the amount of protein in the bird's nest samples. Fig. 3 is a graph showing the results of measurement of the bird's nest enzyme decomposition product 1 by GPC-HPLC. Fig. 4 is a graph showing the results of measurement of the bird's nest enzyme decomposition product 2 by GPC-HPLC. Fig. 5 is a graph showing the results of measurement of the ability to promote collagen production. [Main component symbol description] (none) 18

Claims (1)

201105362 VII. Scope of application for patents: 1. A collagen production promoter, which uses the enzyme decomposition products of bird's nest as an active ingredient. 2. The collagen production promoter according to the first aspect of the patent application, wherein the enzyme decomposition product is a decomposition product of a protease. 3. The collagen production promoter according to claim 1 or 2, wherein the enzyme decomposition product has a molecular weight of 70 to 200,000. 4. The collagen production promoter according to any one of claims 1 to 3, wherein the aforementioned enzyme decomposition product has an average molecular weight of 300 to 15,000 〇 5. as in any one of claims 1 to 4 A collagen production promoter for promoting type I collagen production in dermal fibroblasts of the skin. A skin external composition comprising the collagen production promoter according to any one of claims 1 to 5. 7. For the composition of claim 6 of the patent scope, it is used for skin care. 8. The composition of claim 6 or 7 is used to enhance the elasticity and luster of the skin and to prevent or improve wrinkles. 9. A composition according to any one of claims 6 to 8, which is a cosmetic. 10. The use of an enzyme decomposition product of bird's nest is the use of an enzyme decomposition product of bird's nest for the production of a collagen production promoter for the skin of a mammal including humans. 11. The use of the skin of claim 10, wherein the collagen of the skin S 19 201105362 is used to promote the use of the type 12 collagen of the skin dermal fibroblasts. It is a skin external composition for skin care maintenance. 13. The use of the above-mentioned item 12 of the patent application, wherein the external use of the skin is used to enhance the skin and the use of the _ or the improvement item, wherein the use of the aforementioned item, wherein the aforementioned 14 Any of the enzymes of 10 to 13 is a decomposition product of a protease. 15. If the molecular weight of any of the enzyme decomposition products in the scope of patent application No. 10 to 14 is 70 to 20 〇, 〇〇〇 丫刖 . . 职 职 5 5 5 5 5 5 5 5 5 The use of the enzyme decomposition product has an average molecular weight of 300 to 15,000. ^ For the purpose of applying for the 16th position of the patent scope, the former composition is cosmetics. A method for promoting the production of collagen in a skin, comprising: applying an effective amount of an enzyme decomposition product written by Yan to a skin of a mammal including a human being; 19, such as a compound (four) 18 slave material, The production of collagen, which is described in (4), promotes the production of type I collagen in the dermal fibroblasts. 2. A skin health method comprising: applying an effective amount of the enzyme decomposition product to the skin of a mammal including humans. h.-A way to enhance the skin's miscellaneous and light buckles and to prevent or modify the secret lines. 20 201105362 The method includes: applying an effective amount of the enzyme decomposition product of bird's nest to the skin of a mammal including humans. The method of any one of claims 18 to 21, wherein the aforementioned enzyme decomposition product is a decomposition product of a protease.