CN109771320A - The skin care item and preparation method thereof of bird's nest peptide and plants essential oil compounding - Google Patents
The skin care item and preparation method thereof of bird's nest peptide and plants essential oil compounding Download PDFInfo
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- CN109771320A CN109771320A CN201811580083.0A CN201811580083A CN109771320A CN 109771320 A CN109771320 A CN 109771320A CN 201811580083 A CN201811580083 A CN 201811580083A CN 109771320 A CN109771320 A CN 109771320A
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Abstract
The present invention relates to the skin care item and preparation method thereof that a kind of bird's nest peptide and plants essential oil compound, and belong to technical field of skin care.The skin care item are prepared by the raw material of following weight percent: being prepared by the raw material of following weight percent: the bird's nest peptide of 0.05%-5.0%, the pearl powder of 0.05%-5.0%, the plants essential oil of 0.5%-5.0%, the emulsifier of 0.1%-5.0%, the moisturizer of 0.5%-5.0%, surplus are water.Wherein, bird's nest peptide can reduce and prevent melanin deposition, have good light speckles, beautification and white and repair function；Bird's nest peptide and pearl powder and plants essential oil are cooperated, can reach freckle removing and whitening and repair the function of skin.
Description
Technical field
The present invention relates to technical field of skin care, a kind of compounded more particularly to bird's nest peptide and plants essential oil skin care item and
Preparation method.
Background technique
Skin splash is the common pigmentation of one kind, damage capacitive skin problem.With age, human skin goes out
The probability of existing color spot also obviously increases, this is because being influenced by ultraviolet light irradiation, endocrine disorder, external environment etc., in epidermis
Melanosome number increases, skin pigment metabolic disorder causes.Skin splash especially facial colour spot influences beauty, generates patient
Psychological burden.With the raising of quality of the life, people increasingly focus on skin care.However existing nti-freckle product is mostly with chemical industry
Sintetics is raw material, causes stimulation to skin to a certain extent, increases skin burden, is not suitable for sensitivity skin quality and makes for a long time
With.Most cosmetics contain all kinds of preservatives currently on the market, such product is used for a long time, makes preservative in human skin
Upper accumulation will accelerate skin aging and pigment deposition.Japan capital mansion founds medical university's life safe medical teaching and research room Ji Chuanmin
One professor once pointed out when being interviewed by Japanese Asahi Shimbun, when Determination of Preservatives in Cosmetics is by strong ultraviolet radiation, was easy
Accelerate skin aging, skin is caused wrinkle and color spot occur.
Summary of the invention
Based on this, it is necessary in view of the above-mentioned problems, providing the skin care item and its system of a kind of bird's nest peptide and plants essential oil compounding
Preparation Method adheres to can lightening the stain using the skin care item, delay skin aging.
A kind of skin care item of bird's nest peptide and plants essential oil compounding, are prepared by the raw material of following weight percent:
Bird's nest is beauty and skin care good merchantable brand from ancient times, and bird's nest is resolved into small-molecular peptides by combination zymolysis technique, it is easier to
It is absorbed by the body and plays biological activity.This is because the molecular weight of peptide is small, these small-molecular peptides are not required to digest direct absorption,
With power, carrier, transport, mediator and trophic function.Good nutrient environment can be provided to cell, be conducive to the new old of cell
Metabolism, enables senile cell and impaired epidermis to be replenished in time and repair.For color spot caused by pigmentation, promotion can be passed through
The generation of neoblast replaces senile cell, to reduce melanosome quantity in epidermis；The good battalion that small-molecular peptides provide simultaneously
Environmental benefits are supported in improving the distribution of subcutaneous capillary net, increasing skin blood flow, accelerates melanin discharge, prevents cell generation
Thank to product siltation.
In one of the embodiments, after the sterilized processing of the skin care item 0.4-0.6g/ be packaged in capsule.Pass through
All raw materials are packaged in capsule, good antibacterial effect has been reached, so as to avoid preservative ingredient is added, avoid being added
Preservative by strong ultraviolet radiation when, lead to the problem of accelerate skin aging.
The bird's nest peptide is prepared by the following method to obtain in one of the embodiments:
Enzymatic hydrolysis: taking bird's nest solution, and adjusting temperature is 50-60 DEG C, pH value 7-9, and the first egg of bird's nest dry weight 2-8% is added
White enzyme, after handling 60-100s under the conditions of microwave power 300-600W, enzyme digestion reaction 0.5-3hr；Adjusting temperature again is 45-65
DEG C, pH value 6-9 is added the second protease of bird's nest dry weight 0.2-1%, handles 60- under the conditions of microwave power 300-600W
After 120s, enzyme digestion reaction 0.5-3hr；It is warming up to 90-100 DEG C, enzyme deactivation, obtains enzymolysis liquid；
The first protein enzyme and the second protease is independent is selected from: papain, bromelain, basic protein
Enzyme, trypsase.
Bird's nest peptide is prepared to obtain through the above way, can preferably be absorbed by the skin utilization.
It further include in one of the embodiments, pre-treatment step before the enzymolysis step, the pre-treatment step are as follows:
The bird's nest solution is prepared by the following method to obtain: dry bird's nest taken, is soaked in distilled water by solid-liquid ratio 1g:90-110mL,
Taking-up rinses spare through distilled water after 5-15min；Bird's nest after cleaning is mixed by solid-liquid ratio 1g:20-50mL with distilled water, is set
In 85-95 DEG C of water-bath 3-8min sterilizing to get the bird's nest solution.By above-mentioned pre-treatment, it is more favorable for the hydrolyzed of bird's nest
Journey.
It further include in one of the embodiments, purification step after the enzymolysis step, the purification step are as follows: take enzyme
Liquid is solved, is centrifuged 10-15min under the conditions of 3000-5000r/min, supernatant is taken to pass through molecular cut off 3kDa ultrafiltration membrane ultrafiltration,
The bird's nest Gly-His-Lys are made in filtrate freeze-drying.By above-mentioned purification step, obtained bird's nest peptide can be further purified,
Improve the purity that can be absorbed by the skin using polypeptide.
The plants essential oil is selected from one of the embodiments: Rosa Damascana, Lavender, grape pip essential oil and
At least one of lemon.Other essential oils also can be selected in above-mentioned plants essential oil, with bird's nest peptide and pearl powder phase of the invention
Mutually cooperation.
The emulsifier is selected from one of the embodiments: polyvinyl alcohol, stearyl alcohol, cetostearyl alcohol, stearyl alcohol are poly-
At least one of ether, stearine, Cremophor RH40 and dipropylene glycol.
The moisturizer is selected from one of the embodiments: phytosphingosine, hyaluronic acid, glycerol, propylene glycol and fourth
At least one of glycol.
The pearl powder is obtained by physical crushing in one of the embodiments, and partial size is less than 150,000 mesh.
The invention also discloses the preparation methods for the skin care item that above-mentioned bird's nest peptide and plants essential oil compound, including following step
It is rapid:
Prepared by A phase: water intaking, emulsifier, plants essential oil, moisturizer stir evenly, and are prepared into A phase；
Prepared by B phase: taking bird's nest peptide and pearl powder, be uniformly mixed, be prepared into B phase；
Mixing: being added to A phase for B, is dispersed to uniformly, obtains mixed liquor；
Encapsulation: will after the sterilized processing of above-mentioned mixed liquor be packed into capsule to get.
Compared with prior art, the invention has the following advantages:
The skin care item of a kind of bird's nest peptide and plants essential oil compounding of the invention, by bird's nest peptide, pearl powder and plants essential oil phase
Mutually cooperation, wherein bird's nest peptide can provide good nutrient environment to cell, be conducive to the metabolism of cell, make senile cell
It is able to be replenished in time and repair with impaired epidermis.For color spot caused by pigmentation, the generation generation of promotion neoblast can be passed through
For senile cell, to reduce melanosome quantity in epidermis；The good nutrition environmental benefits that small-molecular peptides provide simultaneously are in changing
Kind subcutaneous capillary net distribution increases skin blood flow, accelerates melanin discharge, prevents products of cellular metabolism from depositing.It will
Bird's nest peptide and pearl powder and plants essential oil cooperate, and can reach freckle removing and whitening and repair the function of skin.
Also, skin care item of the invention have reached good antibacterial effect by the way that all raw materials are packaged in capsule shells,
So as to avoid preservative ingredient is added, when avoiding preservative by strong ultraviolet radiation, it is easy the problem of accelerating skin aging.
Meanwhile this is packaged in the skin care item in capsule shells and is also free of the chemical industry synthesis additive such as pigment, essence, is suitble to be used for a long time.
The preparation method of bird's nest peptide of the invention and the skin care item of plants essential oil compounding, by toward including using water as matrix
Cream is added in skin care item containing solid component (pearl powder), oil-soluble ingredients (plants essential oil) and water soluble ingredient (bird's nest peptide)
Agent forms emulsion oil-in-water, so that product stability is improved, conducive to the storage and use of product.
Detailed description of the invention
Fig. 1 is melanin extinction rate comparing result figure in experimental example.
Specific embodiment
To facilitate the understanding of the present invention, below with reference to embodiment to invention is more fully described.But this hair
It is bright to realize in many different forms, however it is not limited to embodiment described herein.On the contrary, providing these embodiments
Purpose be to make the disclosure of the present invention more thorough and comprehensive.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.
The raw materials used source of following embodiment:
Pearl powder: pure physics production, partial size originate from Zhejiang phoenix Biotechnology Co., Ltd less than 150,000 mesh.
Embodiment 1
A kind of skin care item of bird's nest peptide and plants essential oil compounding, are prepared by the following method to obtain:
One, the preparation of bird's nest peptide.
1, it pre-processes.
It weighs 5g bird's nest to be soaked in distilled water by solid-liquid ratio 1:100 (g:mL), taking-up rinses standby through distilled water after 10min
With；Bird's nest after cleaning is mixed by solid-liquid ratio 1:40 (g:mL) with distilled water, is placed in 90 DEG C of water-bath 5min high-temperature sterilizations, is cooled to
It is spare that room temperature obtains bird's nest mother liquor (i.e. bird's nest solution).
2, it digests.
Above-mentioned bird's nest mother liquor water proof is placed in 65 DEG C of water-baths, pH value is adjusted to 6.5, the spinach of 6% dry bird's nest weight is added
Trailing plants protease (25U/mg, Guangzhou Feng Tian Chemical Co., Ltd.), the microwave treatment 90s for the use of power being 400W, enzyme digestion reaction 2.5
Bath temperature is increased to 95 DEG C of enzyme deactivation 10min after h.
3, it purifies.
After cooling, it is centrifuged 10min under the conditions of 4500r/min, supernatant is taken to pass through molecular cut off 3kDa ultrafiltration membrane ultrafiltration,
Filtrate, which is freeze-dried, is made bird's nest Gly-His-Lys.
Two, prepared by capsule.
Rosa Damascana, cetostearyl alcohol, stearine, glycerol, hyaluronic acid, deionized water are taken, high-speed stirred is equal
It is even, it is prepared into A phase；Then it takes bird's nest peptide, pearl powder to be uniformly mixed, is prepared into B phase, B is added to high-speed rotating A phase and is dispersed
To uniform.
Mass ratio of each component in product is made to be respectively as follows:
0.5g/ loading capsules will be pressed after the sterilized processing of above-mentioned whole mammary fluid, obtain what bird's nest peptide was compounded with Rosa Damascana
Skin care item capsule.
Embodiment 2
A kind of skin care item of bird's nest peptide and plants essential oil compounding, are prepared by the following method to obtain:
One, the preparation of bird's nest peptide.
1, it pre-processes.
It weighs 5g bird's nest to be soaked in distilled water by solid-liquid ratio 1:100 (g:mL), taking-up rinses standby through distilled water after 10min
With；Bird's nest after cleaning is mixed by solid-liquid ratio 1:40 (g:mL) with distilled water, is placed in 90 DEG C of water-bath 5min high-temperature sterilizations, is cooled to
It is spare that room temperature obtains bird's nest mother liquor (i.e. bird's nest solution).
2, it digests.
Above-mentioned bird's nest mother liquor water proof is placed in 55 DEG C of water-baths, pH value is adjusted to 7, the pawpaw of 3% dry bird's nest weight is added
Protease (11U/mg, Nanning Jie Wo biological products Co., Ltd), the microwave treatment 90s for the use of power being 400W, enzymatic hydrolysis
Bath temperature is adjusted to 60 DEG C after reacting 2.5h, and alkali protease (1150 U/ of 0.5% dry bird's nest weight are added in pH value to 8
Mg, Novozyme), the microwave treatment 120s for the use of power being 400W, bath temperature is increased to 95 DEG C of enzyme deactivations after reacting 1.5h
10min。
3, it purifies.
After cooling, it is centrifuged 10min under the conditions of 4500r/min, supernatant is taken to pass through molecular cut off 3kDa ultrafiltration membrane ultrafiltration,
It is freeze-dried and bird's nest Gly-His-Lys is made.
Two, prepared by capsule.
Rosa Damascana, Lavender, cetostearyl alcohol, stearine, glycerol, hyaluronic acid, deionized water are taken,
High-speed stirred is uniformly prepared into A phase；Then it takes bird's nest peptide, pearl powder to be uniformly mixed, is prepared into B phase；B is added to high speed rotation
A phase be dispersed to uniformly.
Mass ratio of each component in product is made to be respectively as follows:
0.5g/ loading capsules will be pressed after the sterilized processing of above-mentioned whole mammary fluid, obtain what bird's nest peptide was compounded with composite essential oil
Skin care item capsule.
Embodiment 3
A kind of skin care item of bird's nest peptide and plants essential oil compounding, are prepared by the following method to obtain:
One, the preparation of bird's nest peptide.
It is prepared according to the method for embodiment 2.
Two, prepared by capsule.
Take Rosa Damascana, Lavender, lemon, cetostearyl alcohol, stearine, hyaluronic acid, plant
Sphingol, deionized water, high-speed stirred is uniform, is prepared into A phase；Then it takes bird's nest peptide, pearl powder to be uniformly mixed, is prepared into B
Phase；B is added to high-speed rotating A phase to be dispersed to uniformly.
Mass ratio of each component in product is made to be respectively as follows:
0.5g/ loading capsules will be pressed after the sterilized processing of above-mentioned whole mammary fluid, obtain what bird's nest peptide was compounded with composite essential oil
Skin care item capsule.
Experimental example
The above-mentioned skin care item capsule being prepared is taken to carry out freckle effect test, specific as follows:
4 groups will be divided into 30-50 years old 60 Chinese womens, every group of 15 people are (commercially available by embodiment 1-3 and comparative example
Certain nti-freckle product) skin care item be applied to every group, each primary totally 28 days daily morning and evening, wherein subject required: 1, non-pregnancy
Or nursing period, 2, nearly three months and entire test phase non-oral contraceptive.
As a result as follows:
1, color spot dispels rate.
With direct visual perception subject's color spot recession area, as a result as shown in the table.
The test of 1 nti-freckle repairing effect of table
| Nti-freckle test | Compare example | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Number of cases | 15 | 15 | 15 | 15 |
| It is basic to cure | 1 | 1 | 3 | 4 |
| Significantly | 3 | 3 | 4 | 5 |
| It improves | 4 | 5 | 5 | 5 |
| In vain | 7 | 6 | 3 | 1 |
| Total effective rate | 53% | 60% | 80% | 93% |
Basic to cure: color spot disappears substantially, and naked eyes regard color spot area recession > 80%；
Significant: color spot is obviously thin out, and naked eyes regard color spot area recession > 50%；
Improve: color spot is thin out, and naked eyes regard color spot area recession > 20%；
Invalid: Colour patterns are unobvious, and naked eyes regard color spot area recession < 20%；
It improves, is significant, basic cure belongs to effectively.
2, melanin extinction rate is tested.
Using German CK company dermal melanin tester MX 18, measurement uses embodiment 1-3 whitening spot-removing capsule respectively
Skin care item and the skin care item of comparative example before use, 1 week after use, 2 weeks, 3 weeks, the changes of 4 weeks face's fixed position melanin amounts
Change, and calculate extinction rate, is compared with melanin extinction rate, as a result as shown in Figure 1.
Above-mentioned table 1 and Fig. 1's the result shows that, the skin care item capsule of embodiment 1-3 can be obviously improved skin splash and ask
Topic, is had the effect of the skin care item capsule of freckle removing and whitening, especially embodiment 3, is reached using macroscopic nti-freckle effective percentage after 4 weeks
93%, there is remarkable result.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. the skin care item of a kind of bird's nest peptide and plants essential oil compounding, which is characterized in that prepared by the raw material of following weight percent
It forms:
2. the skin care item of bird's nest peptide according to claim 1 and plants essential oil compounding, which is characterized in that the skin care item warp
0.4-0.6g/ are packaged in capsule after sterilization treatment.
3. the skin care item of bird's nest peptide according to claim 1 or 2 and plants essential oil compounding, which is characterized in that the bird's nest
Peptide is prepared by the following method to obtain:
Enzymatic hydrolysis: taking bird's nest solution, and adjusting temperature is 50-65 DEG C, pH value 7-9, and the first protein of bird's nest dry weight 2-8% is added
Enzyme, after handling 60-100s under the conditions of microwave power 300-600W, enzyme digestion reaction 0.5-3hr；Adjusting temperature again is 45-65 DEG C,
PH value is 6-9, and the second protease of bird's nest dry weight 0.2-1% is added, handles 60-120s under the conditions of microwave power 300-600W
Afterwards, enzyme digestion reaction 0.5-3hr；It is warming up to 90-100 DEG C, enzyme deactivation, obtains enzymolysis liquid；
The first protein enzyme and the second protease is independent is selected from: papain, bromelain, alkali protease, pancreas
Protease.
4. the skin care item of bird's nest peptide according to claim 3 and plants essential oil compounding, which is characterized in that the enzymolysis step
It before further include pre-treatment step, the pre-treatment step are as follows: the bird's nest solution is prepared by the following method to obtain: taking dry swallow
Nest is soaked in distilled water by solid-liquid ratio 1g:90-110mL, and taking-up rinses spare through distilled water after 5-15min；Swallow after cleaning
Nest is mixed by solid-liquid ratio 1g:20-50mL with distilled water, is placed in 85-95 DEG C of water-bath 3-8min sterilizing to get the bird's nest solution.
5. the skin care item of bird's nest peptide according to claim 3 and plants essential oil compounding, which is characterized in that the enzymolysis step
It later further include purification step, the purification step are as follows: enzymolysis liquid is taken, is centrifuged 10-15min under the conditions of 3000-5000r/min,
Take supernatant by molecular cut off 3kDa ultrafiltration membrane ultrafiltration, the bird's nest Gly-His-Lys are made in filtrate freeze-drying.
6. the skin care item of bird's nest peptide according to claim 1 or 2 and plants essential oil compounding, which is characterized in that the plant
Essential oil is selected from: at least one of Rosa Damascana, Lavender, grape pip essential oil and lemon.
7. the skin care item of bird's nest peptide according to claim 1 or 2 and plants essential oil compounding, which is characterized in that the emulsification
Agent is selected from: polyvinyl alcohol, stearyl alcohol, cetostearyl alcohol, stereth, stearine, Cremophor RH40 and two
At least one of propylene glycol.
8. the skin care item of bird's nest peptide according to claim 1 or 2 and plants essential oil compounding, which is characterized in that the moisturizing
Agent is selected from: at least one of phytosphingosine, hyaluronic acid, glycerol, propylene glycol and butanediol.
9. the skin care item of bird's nest peptide according to claim 1 or 2 and plants essential oil compounding, which is characterized in that the pearl
Powder is obtained by physical crushing, and partial size is less than 150,000 mesh.
10. the preparation method of the described in any item bird's nest peptides of claim 1-9 and the skin care item of plants essential oil compounding, feature exist
In, comprising the following steps:
Prepared by A phase: water intaking, emulsifier, plants essential oil, moisturizer stir evenly, and are prepared into A phase；
Prepared by B phase: taking bird's nest peptide and pearl powder, be uniformly mixed, be prepared into B phase；
Mixing: being added to A phase for B, is dispersed to uniformly, obtains mixed liquor；
Encapsulation: will after the sterilized processing of above-mentioned mixed liquor be packed into capsule to get.
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Application publication date: 20190521 |
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| RJ01 | Rejection of invention patent application after publication |