KR20120106703A - Collagen production enhancer, and external composition for skin and cosmetic each comprising same - Google Patents

Abstract

The collagen production promoter according to the present invention contains an enzyme decomposition product of lead as an active ingredient. According to the present invention, there is provided an agent which can be produced efficiently, has high stability, and has excellent production of collagen type I, and external preparations and cosmetics including the same.

Description

Collagen production promoters, external skin compositions and cosmetics including the same {COLLAGEN PRODUCTION ENHANCER, AND EXTERNAL COMPOSITION FOR SKIN AND COSMETIC EACH COMPRISING SAME}

This application is based on the JP Patent application 2009-148754 of an earlier Japanese patent application (application date: June 23, 2009), and claims the benefit of that priority, The whole content of this indication is taken in here as a reference.

The present invention relates to a collagen production promoter comprising as an active ingredient an enzyme degradation product of Yeonwa. Specifically, the present invention relates to a collagen production promoter, which promotes type I collagen production in skin dermal fibroblasts. The present invention also relates to a composition for external skin and a cosmetic comprising an enzymatic degradation product of lead as its active ingredient.

Skin problems such as skin roughness, wrinkles, sagging skin, and decreased elasticity are of particular concern for women. These skin troubles are usually considered to be caused by environmental factors, factors such as aging, and psychological factors such as stress. In particular, it can be said that environmental factors that adversely affect the skin due to the increase of ultraviolet rays, the drying of the living environment due to the multi-use of the air conditioner, and the like are increasing.

Skin tissue consists of the epidermis, dermis and subcutaneous tissue. The dermis contains a lot of collagen and hyaluronic acid, and is largely involved in skin's water retention and elasticity. Collagen and hyaluronic acid are known to produce dermal fibroblasts present in the dermis.

Collagen accounts for about 30% of the proteins present in human living bodies, 70% of which is known to be present in the dermis. Collagen present in the dermis includes type I collagen and type III collagen, but the main one is type I collagen.

Background Art Conventionally, for the purpose of improving skin elasticity and gloss, and preventing wrinkles, cosmetics containing collagen have been used for external use, and a method of directly supplementing collagen with skin has been widely used. However, when collagen is supplemented externally, it is difficult to increase the amount of collagen in the dermis of the skin. Therefore, it is not always sufficient to improve skin elasticity, glossiness, and wrinkle prevention.

When the skin is exposed to light including ultraviolet rays, it is known that collagen, which occupies most of the extracellular matrix of the dermis, is markedly reduced, causing disruption of its fiber bundles. For this reason, increasing the amount of collagen in the dermis is said to be useful for improving or preventing wrinkles (Document 1 ("Experimental Method for the Development of Functional Cosmetic Materials" (supervised by Genji Amokawa), published in 2007, published by CMC) , Pages 45-47). In addition, it is shown that collagen synthesis of dermal fibroblasts is greatly reduced by irradiation with medium-wave ultraviolet (UVB). Here, as an example of the type I collagen synthesis promoter, a vitamin C derivative, a milk serum component, etc. are illustrated.

On the other hand, in Document 2 [Akiko Kojima-Yuasa, et al., Fragrance Journal, April, 2009, No.346 (Vol.37, No.4), pp.103-], suppressing the decrease in collagen production ability It is useful for improving QOL, and it has been shown that a decrease in the amount of collagen in the skin causes wrinkles and sagging.

As a production accelerator of type I collagen, many reports have been made so far by paying attention to a specific plant-derived component (for example, Japanese Patent Laid-Open No. 2008-105984 or WO 2004/085429).

Lotus is a nest that Geumsayeon makes its saliva in the form of a thread, and it is known that it contains many glycoproteins and contains little lipid. Yeonwa is conventionally used for edible materials in Chinese cuisine, raw materials for herbal medicine, and the like. In recent years, health foods using lead as raw materials have also been developed, but application examples for external use such as external preparations and cosmetics are still insignificant.

Japanese Unexamined Patent Publication No. 2003-95961 discloses an oral skin care accelerator using an enzymatic degradation product of a pontoon. However, only oral use is disclosed here, and neither description nor suggestion is available about the use for external use. In addition, the effect here is to improve the skin's moisture, to obtain a fine grained skin, and to describe the production promoting ability of type I collagen accompanied with the improvement of the elasticity and the luster of the skin and the improvement of the wrinkles.

WO 99/022709 discloses a cosmetic comprising a water-containing solvent extract, especially a hot water extract of a lotus fruit. This cosmetic can be used for skin care and is used for external use. Moreover, it also discloses that a hydrate extract of yeonwa exhibits the collagen synthesis promoting effect in skin cells. However, the extract used here removes solids by hot water extraction of lead and is obviously different from lead and enzyme decomposition products which are used as they are by enzymatically treating the whole lead. In addition, it was thought that there was room for improvement as far as the inventors think about the effects.

The present inventors have found that the enzyme digestion of yeonwa has an excellent production promoting effect of type I collagen in skin dermal fibroblasts. This effect was more remarkable than the effect shown in the hot water extract of the yeonwa, and the effect was more remarkable in the enzyme digested product which advanced the enzyme decomposition further. This invention is based on these knowledge.

Accordingly, an object of the present invention is to provide an accelerator for the production of collagen type I, which is highly stable and has excellent stability, and an external preparation and a cosmetic containing the same.

The collagen production promoter according to the present invention contains an enzyme decomposition product of lead as an active ingredient.

According to one preferred embodiment of the invention, the enzymatic digest is a digest by protease.

According to one more preferred aspect of the present invention, the molecular weight of the enzymatic digest is 70 to 200,000.

According to one more preferred aspect of the present invention, the average molecular weight of the enzyme digest is 300 to 15,000.

According to a preferred embodiment of the present invention, the collagen production promoter according to the present invention is for promoting type I collagen production in dermal dermal fibroblasts.

According to another aspect of the present invention, there is provided an external skin composition comprising a collagen production promoter according to the present invention. Preferably, this composition is used for skin care, and more preferably used to improve skin elasticity and gloss, and to prevent or improve wrinkles.

According to another more preferred aspect of the present invention, the external composition for skin according to the present invention is a cosmetic.

According to another aspect of the present invention, there is provided the use of an enzymatic degradation product of a kite for producing a collagen production promoter in the skin of a mammal, including a human. According to another aspect of the present invention, there is provided the use of an enzymatic digest with a kite for preparing a skin care composition for skin care.

According to still another aspect of the present invention, there is provided a method for promoting production of collagen in the skin, comprising applying an effective amount of an enzyme digest which is an active ingredient of the present invention to the skin of a mammal, including a human. According to yet another aspect of the present invention, there is provided a skincare method comprising applying an effective amount of the active ingredient of the present invention to the skin of a mammal, including a human. Similarly, there is also provided a method of improving the elasticity and glossiness of the skin and preventing or improving wrinkles, comprising applying an effective amount of the active ingredient of the present invention to the skin of a mammal, including a human.

According to the present invention, the production of collagen in human dermal dermal fibroblasts can be significantly promoted compared to the conventional one by using the enzyme degradant of lead as an active ingredient. In addition, based on the ability of the dermal fibroblasts to promote collagen production, enzyme degradants can be used for external skin applications or cosmetics to enhance skin elasticity, improve skin luster, and prevent or reduce wrinkles. Can be. In addition, it is possible to promote collagen production in the skin in which the collagen synthesis is deteriorated in the skin after sunburn, thereby improving skin elasticity, glossiness, and wrinkle improvement. In this manner, according to the present invention, it is possible to improve the consumer QOL. In addition, since the enzyme decomposed | degraded product used uses lead which is well-known also as an edible material, safety is high and it is easy to be accepted by a consumer. In addition, since the enzyme decomposed product can be obtained by enzymatically treating the lead as it is, it can be produced efficiently, and the production cost can be suppressed low.

1 is a graph showing a measurement result of amount of sialic acid in a lead and a sample.
2 is a graph showing the results of measurement of the amount of protein in lead and samples.
3 is a graph showing the results of measurement of lead and enzyme digest 1 by GPC-HPLC.
4 is a graph showing the results of measurement of lead and enzyme digest 2 by GPC-HPLC.
5 is a graph showing the measurement results of collagen production promoting ability.

Active ingredient

As described above, the collagen production promoter according to the present invention contains an enzyme decomposition product of lead as an active ingredient.

Yeonwa, which is the raw material of the enzyme decomposition product, is a nest in which Geumsayeon has his saliva in the form of a thread, and contains a large amount of glycoprotein as its component, and contains little lipid. Geumsayeon refers to the larvae belonging to the Collocalia of Apodidae, for example, Apodidae Collocalia francica, Apodidae Collocalia salangana, Apodidae Collocalia francang, Apodidae Collocalia fuciphaga, Apodidae Collocalia inexpectata, Apodidae Collocalia vestita, Apodidae Collocalia vestita, Apodidae Collocalia fuciphaga Apodidae Collocalia esculenta, Apodidae Collocalia maximus, and the like.

In this invention, a commercial item can be used for a lead. In general, there are various types of lead commercially available, from decontamination and washing of hairs and dung, to remnants of bleaching and washing by collecting remnants of kite, but the kites used in the present invention are used for pretreatment. Therefore, it is preferable to use lead that is not subjected to excessive washing or bleaching.

The enzymatic digestion of the confectionary means that it is obtained by decomposing the confectionary by applying an enzymatic reaction, and can be obtained by decomposing the confectionery or its processed product using a protease, a complex enzyme containing a protease, or the like. As an enzyme used for a decomposition process here, Preferably it is a protease, More preferably, it is pancreatin.

The enzyme decomposed product of the edible can usually be subjected to heat sterilization treatment first and then subjected to enzyme treatment in water. The conditions of the sterilization treatment can be appropriately set with reference to the usual heat sterilization conditions, and the conditions of the enzyme treatment can also be appropriately set according to the type of enzyme to be used and the state of the smoke.

More specifically, for example, the enzyme decomposed product of the lead can be produced as follows.

For example, 10 to 50 times the mass of water is added to the pulverized lead, followed by swelling, followed by heat sterilization at 60 to 130 ° C. for 5 seconds to 30 minutes. Thereafter, an appropriate amount of enzyme is added as it is, and the enzyme reaction is carried out for 0.5 to 48 hours at the optimum pH and the optimum temperature of the enzyme. After the end of the enzyme reaction, the enzyme is deactivated by heat treatment or the like, and the reaction solution is filtered to remove the insolubles, whereby the enzymatic degradation product of the lead can be obtained. In addition, this enzyme decomposition product can be dried and powdered as needed. In addition, at each said process, operation, such as pH adjustment, decolorization, deodorization, can also be performed suitably.

As another example, in the case of enzymatically decomposing the treated product of lead, 10 to 100 times the mass of water is added to the mill ground to a particle diameter of 2 mm or less, preferably 150 μm or less, and 1 to 60 ° C, After standing for 0.5 to 48 hours or swelling with stirring, heat sterilization treatment was performed at 60 to 130 ° C. for 5 seconds to 30 minutes, and filtration as necessary, and the obtained solution was added to the enzyme treatment in the same manner as described above to produce an enzyme with lead. Decomposition can be obtained.

In the method for producing an enzymatic digestion product, the longer the reaction time, the more the protein is degraded, so that an enzymatic digestion product containing a lower molecular protein is obtained. In the present invention, the enzymatic digestion product in which the enzymatic reaction is further advanced is preferably set at an enzyme reaction time of 0.5 to 48 hours, preferably 3 to 36 hours, more preferably about 24 hours.

In this way, 70-200,000 are preferable and, as for the molecular weight of the enzyme degradation product of the lead, 70-180,000 are more preferable. More preferably, the molecular weight is 70 to 150,000, even more preferably 70 to 120,000.

Moreover, 300-100,000 are preferable and, as for the average molecular weight (weight average molecular weight) of the enzyme decomposition | strain of lead, 300-70,000 are preferable. More preferably, the average molecular weight is 300 to 50,000, still more preferably 300 to 30,000, still more preferably 300 to 20,000, particularly preferably 300 to 15,000.

External skin  Composition

The collagen production promoter according to the present invention can be used alone as it is, but can be contained as an additive in various external skin compositions such as cosmetics, pharmaceuticals, quasi-drugs, and the like, and a composition having a collagen production promoting effect can be obtained. The obtained composition can be effectively used for skin care, preferably for improving skin elasticity and shine, and for preventing or improving wrinkles.

The enzyme digested product of Yeonwa, which is an active ingredient according to the present invention, has a production promoting activity of type I collagen in human dermal dermal fibroblasts (test results of Examples described later).

Therefore, in the present invention, the production promoting activity of collagen means an action of promoting or improving the production capacity of type I collagen of fibroblasts of human skin dermis, which means that the amount of collagen in human skin dermis can be increased. do. It is well known to those skilled in the art that by increasing the amount of collagen in the dermis, wrinkle improvement and prevention effects can be obtained, and the elasticity (elasticity) of the skin can be improved and the skin gloss can be improved (for example, the above-mentioned) A document ["Experimental methods for the development of functional cosmetic materials" (supervised by Genji Amokawa), published in 2007, published by CMC, pages 45-47 (Non-Patent Document 1)], or by Akiko Kojima-Yuasa, et. al., Fragrance Journal, April, 2009, No. 346 (Vol. 37, No. 4), pp. 103- (Non-Patent Document 2)]. Therefore, since it has been confirmed that the active ingredient according to the present invention has collagen production promoting activity in the dermis, the active ingredient according to the present invention is skincare, preferably improves the elasticity and luster of the skin, and prevents wrinkles or It is clear that it is effective for improvement.

In addition, in the present specification, the "prevention or improvement" of the skin or skin condition such as elasticity, shine, wrinkles, etc. of the skin is meant to include control of the condition, delay of progression, alleviation, prevention of onset, prevention of recurrence, inhibition, and the like. Used.

Therefore, as described above, according to another aspect of the present invention, there is provided an external skin composition comprising a collagen production promoter according to the present invention. Preferably, the external skin composition is provided as a medicine, quasi-drug, or cosmetic, and more preferably as a cosmetic.

In the composition for external application for skin (particularly cosmetics) according to the present invention, the content (in terms of buildings) of lead and enzyme decomposition products which are active ingredients is not particularly limited, and varies depending on the form of the composition, but is generally 0.001 to 20% by weight, Preferably it is 0.005 to 5 weight% of range, and it can change suitably according to the form of a composition.

Alternatively, the content (in terms of buildings) of lead and enzyme decomposition products in the external skin composition according to the present invention is 0.01 mg or more per day, more preferably 0.01 mg to 2 mg, so as to be applicable to the skin per square cm. It is preferable.

The external composition for skin according to the present invention contains the active ingredient, and the accelerator according to the present invention may further comprise other optional ingredients, so long as the production promoting effect of collagen is not impaired. As such optional components, for example, various components commonly used in skin external preparations and cosmetics, for example, moisturizers, lower alcohols, polyhydric alcohols, sugars, surfactants, buffers, emulsifiers, stabilizers, thickeners, antioxidants, antiseptics Fungicides, chelating agents, pH adjusters, fragrances, pigments, ultraviolet absorbers, ultraviolet scatterers, vitamins, amino acids, anti-inflammatory agents, water, peptides, sugar alcohols, enzymes, plant extracts, antioxidants, talc, clay, Pollen, pearls, and the like.

Examples of the moisturizing agent include polyethylene glycol, polypropylene glycol, glycerin, propylene glycol, sorbitol, acidic mucopolysaccharides such as hyaluronic acid and chondroitin sulfate, amino acids, collagen, elastin, and the like.

As lower alcohol, ethanol, propanol, isopropanol, etc. are mentioned, for example.

As a polyhydric alcohol, glycerin, pentaerythritol, dipentaerythritol, ethylene glycol, propylene glycol, polypropylene glycol, 1, 3- butylene glycol, etc. are mentioned, for example.

Examples of the sugars include glucose, maltose, lactose, D-glucuronic acid, sucrose, D-sorbet, sorbitan, saccharides such as cellulose, starch, oligosaccharides, polysaccharides and derivatives thereof.

The external composition for skin according to the present invention is formulated by appropriately mixing and adjusting a collagen production promoter including lead and an enzyme decomposition product according to a known method, such as a lotion, an emulsion, a cream, a moisturizing solution, a pack, a lotion, and a bath. It can be made into the usual product form in various cosmetics, such as a pigment, a body lotion, and a body cream. The external composition for skin according to the present invention is further used for makeup cosmetics such as foundations, lipsticks, eye shadows, ball touches, medicinal cosmetics such as UV-protecting products and deodorizing cosmetics, and for hair for shampoos, hair rinses, and barbers. It can also be used as cosmetics, skin cleaners, bath cosmetics for baths and the like.

According to another aspect of the present invention, as described above, there is provided the use of an enzymatic degradation product of a kite to prepare a collagen production promoter in the skin of a mammal, including a human. Here, the promotion of collagen production in the skin is preferably to promote the production of type I collagen in dermal dermal fibroblasts.

According to another aspect of the present invention, as described above, there is provided the use of an enzymatic degradation product of a kite for producing a skin care composition for skin care. The composition for external skin is preferably used for the improvement of elasticity and luster of the skin, and the prevention or improvement of wrinkles. In addition, the composition for external skin is preferably a cosmetic.

According to still another aspect of the present invention, as described above, the method for promoting the production of collagen in the skin, comprising applying an effective amount of an enzyme digest which is an active ingredient of the present invention to the skin of a mammal including a human, Is provided. In addition, an "effective amount" is an amount sufficient for being able to exhibit the production | stimulation activity of collagen in a desired area | region in a body by application here. In addition, the production | stimulation of collagen production in the skin said here is what promotes production of type I collagen in dermal dermal fibroblasts.

According to yet another aspect of the present invention, there is provided a skincare method comprising applying an effective amount of the active ingredient of the present invention to the skin of a mammal, including a human. Similarly, there is also provided a method of improving the elasticity and glossiness of the skin and preventing or improving wrinkles, comprising applying an effective amount of the active ingredient of the present invention to the skin of a mammal, including a human. In addition, an "effective amount" is an amount sufficient for obtaining a desired effect here.

In addition, in this specification, expression of the value using "about" and "degree" is the meaning including the change of the value which a person skilled in the art can accept in achieving the objective by setting the value. For example, it means that the variation within 20%, preferably within 10%, and more preferably within 5% of a predetermined value or range can be tolerated.

[Example]

Although this invention is demonstrated concretely based on the following examples, this invention is not limited to these examples.

(One) Yeonwa  Manufacture of samples

(1-1) Yeonwa  enzyme resolvent  One

Commercially available unbleached lead was pulverized with a pulverizer to obtain 100 mesh passes (150 µm or less in diameter) of lead and powder, and about 50 times (mass) of water was added to the lead and powder for swelling at 5 ° C. for 20 hours. After that, heat sterilization was performed at 121 ° C. for 15 minutes.

After cooling the obtained process liquid, pH was adjusted, and the protease containing enzyme (brand name "pancreatin F", Amano Seiyaku Co., Ltd. make) of 2 mass% with respect to lead and powder was added, and it was made into 3 at 45 degreeC. The reaction was carried out for a time.

After adjusting this enzyme reaction liquid to pH 7.0, it heated at 90 degreeC for 5 minutes, inactivated the enzyme, and filtered, and collect | recovered the filtrate. The filtrate was concentrated and freeze-dried to obtain "lead and enzyme digest 1".

(1-2) Yeonwa  enzyme resolvent  2

Except having made the time of the enzyme reaction into 24 hours, it carried out similarly to the enzyme digest 1 of the said (1-1) section, and obtained the "lead and enzyme digest 2".

(1-3) Yeonwa Heat number  extract( Comparative example )

40 g of commercially available unbleached lead (dried) was ground by a mill, and the milled product was added to 1000 ml of distilled water, followed by extraction for 2 hours under reflux. After heating to reflux, after standing still, the supernatant was aliquoted and filtered to recover the filtrate. The residue was further added to 1000 ml of distilled water in the same manner as above, and after re-extracting under heating and reflux, the solid residue was removed by filtration to recover the filtrate. The obtained filtrate (extract) was combined and concentrated under reduced pressure, and the obtained concentrate was freeze-dried to obtain an off-white "Yellow and hot water extract" (Comparative Example).

(2) Yeonwa  Analysis of samples

The amount of sialic acid and the amount of protein in each of the obtained lead samples (lead and enzyme digest 1, lead and enzyme digest 2 and lead and hydrothermal extract) were measured as follows.

The amount of sialic acid was measured as free N-acetylneuraminic acid by high performance liquid chromatography after each hydrolysis of each sample.

The amount of protein was measured using a protein assay kit from Bio Rad, based on the Brad ford method.

The result was as showing to FIG. 1 and FIG.

Next, in order to measure the molecular weight and distribution of the protein contained in the lead and enzyme digest 1 and the lead and enzyme digest 2, analysis by GPC was carried out under the following conditions.

[Measurement condition of HPLC]

Column: Shodex Asahipak GS320HQ (φ7.6 × 300 mm)

Column temperature: 35 ℃

Mobile phase: 50 mM CH ₃ COONH ₃ pH 6.7

Flow rate: 0.4 ml / min

Sample injection volume: 10 μl

Detector: ultraviolet spectrometer (measured at UV 220 nm)

[Molecular Weight Markers Used]

? BSA MW 66,000

? Gly-gly MW 132.1

? Gly-gly-gly MW 189.17

L-aspartic acid MW 133.1

DL-aspartic acid MW 150.1

N-acetylneuraminic acid MW 309.3

The measurement result was as showing to FIG. 3 and FIG.

In addition, the molecular weight of the protein contained in the lead and the enzymatic digest was evaluated using Western blotting. Specifically, first, the amount of protein in the lead and the enzyme digested product was confirmed according to the above-described measuring method. Proteins (100 μg) derived from lead and enzyme digests were separated by 10-15% polyacrylamide using SDS-PAGE. Proteins developed on the gel were transferred to an immunoassay (Immuno-blot) PVDF membrane (Polyvinylidene difluoride, available from Bio-Lad Lab). The PVDF membrane was blocked for 1 hour in PBS containing 3% skim milk and 0.1% Tween-20 and visually determined.

In addition, in evaluation of the molecular weight of a protein, Guo, C. T., Suzuki Y., et al., Antiviral Res. 70, pp. 140-146 (2006).

From these results, it turned out that the protein molecular weight contained in lead and an enzyme degradation product exists in the range of about 70-120,000 (In addition, an upper limit may be about 200,000 depending on evaluation conditions).

From the obtained results, it was found that the average molecular weight of the protein in lead and enzyme digest 1 was about 55,000. In addition, it turned out that the average molecular weight of the protein in lead and the enzyme degradation product 2 is about 33,000.

(3) production of collagen Facilitating  Measure

Normal human dermal dermal fibroblasts (KF-4109, available from Clavosa), 2 × 10 ⁴ cells in a 96-hole plate using DMEM medium containing 0.5% calf serum (FBS) (obtained from SIGMA) Sowing was carried out with density. After 24 hours of sowing, the cells were exchanged with DMEM medium containing 0.5% FBS containing each kite and sample at a predetermined concentration and incubated for an additional 24 hours.

As positive control (P.C.), magnesium ascorbic acid phosphate (VC-PMg) was used.

The culture medium supernatant was recovered after the culture, and the amount of type I collagen in the supernatant was measured by ELISA. In the ELISA method, an anti-human collagen type I antibody (Rabit) is used as a primary antibody, and histopine PO (Rabit) is used as a secondary antibody, and a coloring agent is further used. Absorbance of 405 nm was measured with a microplate reader using 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).

The amount of type I collagen in the medium was calculated as the amount of type I collagen produced per unit protein by dividing the amount of type I collagen in the medium by the amount of protein in the whole cells.

The result was as shown in FIG.

From the results, it was recognized that enzymatic digestion of yeonwa significantly increased type I collagen production in human dermal dermal fibroblasts.

Claims (22)

Collagen production promoter which uses enzyme degradation product of lead as active ingredient. The collagen production promoter according to claim 1, wherein the enzymatic digest is a digest by protease. The collagen production promoter according to claim 1 or 2, wherein the molecular weight of the enzyme digest is 70 to 200,000. The collagen production promoter according to any one of claims 1 to 3, wherein the average molecular weight of the enzyme digest is 300 to 15,000. The collagen production promoter according to any one of claims 1 to 4, for promoting type I collagen production in dermal dermal fibroblasts. The external composition for skin containing the collagen production promoter in any one of Claims 1-5. The skin external composition according to claim 6, which is used for skin care. The composition for external application of skin according to claim 6 or 7, which is used to improve skin elasticity and gloss, and to prevent or improve wrinkles. The skin external composition according to any one of claims 6 to 8, which is a cosmetic. Use of Enzyme Degradants of Edible To Prepare Collagen Production Promoters in the Skin of Mammals, Including Humans. Use according to claim 10, wherein the enzymatic digestion of edibles in which production of collagen in the skin promotes production of type I collagen in dermal dermal fibroblasts. Use of Enzyme Degradants of Smokeweed for Preparation of Skin Care Compositions for Skin Care. 13. The use of an enzyme degradant with lead according to claim 12, wherein the external skin composition is used to improve skin elasticity and shine and to prevent or improve wrinkles. Use according to any one of claims 10 to 13, wherein the enzymatic digest is an enzymatic digest with a protease. The use of the enzyme degradant according to any one of claims 10 to 14, wherein the molecular weight of the enzyme degradant is 70 to 200,000. The use of lead enzyme degradants according to any one of claims 10 to 15, wherein the average molecular weight of the enzyme digest is 300 to 15,000. The use of the enzyme decomposed product of the edible fruit according to any one of claims 10 to 16, wherein the external skin composition is a cosmetic. A method for promoting the production of collagen in the skin, comprising applying an effective amount of the enzyme decomposed product to the skin of a mammal including a human. The method of claim 18, wherein the promoting production of collagen in the skin promotes the production of type I collagen in dermal dermal fibroblasts. A skin care method comprising applying an effective amount of an enzyme decomposed product to a skin of a mammal including a human. A method for improving elasticity and luster of skin, preventing or improving wrinkles, comprising applying an effective amount of an enzyme decomposed product of a kite to skin of a mammal including a human. 22. The method of any one of claims 18 to 21, wherein the enzymatic digest is a digest by protease.

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