JP2021031451A - Ginkgo leaf-derived fermentation composition - Google Patents

Abstract

To provide a composition showing an excellent blood flow promoting effect.SOLUTION: The present invention relates to a fermentation composition that contains the ginkgo leaves fermented with acetic bacteria, or extract thereof, and relates to a blood flow promoter containing the fermentation composition.SELECTED DRAWING: None

Description

The present invention relates to a fermentation composition derived from Ginkgo biloba.

Ginkgo biloba is a gymnosperm belonging to the genus Ginkgoaceae and is widely distributed in temperate regions around the world. Its birth is as old as more than 200 million years ago, and while other ginkgoaceae plants have become almost extinct during the ice age, ginkgo continues to inhabit, so it is called a living fossil. Ginkgo is used as a typical roadside tree in Japan, and has also been used as timber for a long time. Ginkgo biloba seeds are popular as ginkgo biloba as one of the ingredients that color autumn in Japan.

Ginkgo biloba (Ginkgo biloba) is known to contain flavonoids and terpenoids as active ingredients. Ginkgo biloba is used as a health food with the expectation of improving blood flow (promoting blood flow), preventing dementia, and improving cognitive function. Furthermore, Ginkgo biloba is expected as a cosmetic product to have an antioxidant effect and an inhibitory effect on lipid peroxidation, in addition to an effect of improving blood flow.

Ginkgo biloba-derived proanthocyanidins are said to be effective in improving blood flow (Patent Document 1). Patent Document 2 states that by combining Ginkgo biloba extract and proanthocyanidin, which is normally significantly lost during the production of Ginkgo biloba extract, physiological activity such as blood flow improving action of Ginkgo biloba extract can be enhanced. It discloses that a combination of such ingredients is used for an external preparation or the like. Patent Document 3 discloses that Apocynum venetum leaf extract having an effect of improving blood flow velocity is mixed with Ginkgo biloba extract and licorice extract and used as a capillary circulation improving agent. However, the development of a composition effective for improving blood flow is still desired.

Acetobacter is an aerobic bacterium having the property of accumulating (oxidative fermentation) alcohol and sugar as organic acids and sugar acids by incomplete oxidation. Acetobacter is mainly classified into the genus Acetobacter and the genus Gluconobacter. Acetobacter is a non-spore-forming obligate aerobic bacillus, and is known to be a bacterium that has been widely used in the production of vinegar since ancient times and a bacterium that produces cellulose or β-glucan. Gluconobacter is also a non-spore-forming obligate aerobic bacillus, which can decompose various sugars, for example, glucose can be decomposed to produce gluconic acid. By using cellulose produced by Acetobacter as a material for cosmetics (Patent Document 4), or by using β-glucan produced by Acetobacter as a base material for cosmetics (Patent Document 5). , The use of acetobacter in the field of cosmetics manufacturing is also advancing.

Japanese Unexamined Patent Publication No. 2004-123622 Japanese Unexamined Patent Publication No. 2006-11617 Japanese Unexamined Patent Publication No. 2008-069114 Japanese Unexamined Patent Publication No. 4-268301 Japanese Unexamined Patent Publication No. 2008-169188

An object of the present invention is to provide a composition having an excellent blood flow promoting action.

As a result of diligent studies to solve the above problems, the present inventors have found that the blood flow promoting action of Ginkgo biloba can be enhanced by fermenting Ginkgo biloba with acetobacter, and have completed the present invention. It was.

That is, the present invention includes the following.
[1] A fermentation composition containing a fermented product of Acetobacter in Ginkgo biloba or an extract thereof.
[2] The fermentation composition according to the above [1], wherein the acetobacter belongs to the genus Acetobacter or the genus Gluconobacter.
[3] The fermentation composition according to the above [1] or [2], wherein the acetobacter is Acetobacter orleanensis or Gluconobacter frateurii.
[4] The fermentation composition according to any one of [1] to [3] above, wherein the extract is an extract from a solvent containing alcohol and / or water.
[5] The fermentation composition according to the above [4], wherein the alcohol is 1,3-butylene glycol or ethanol.
[6] A blood flow promoter containing the fermentation composition according to any one of the above [1] to [5].
[7] A cosmetic for promoting blood flow, which comprises the fermentation composition according to any one of the above [1] to [5].
[8] A pharmaceutical for promoting blood flow, which comprises the fermentation composition according to any one of the above [1] to [5].
[9] A method for producing a fermentation composition in which the blood flow promoting action of Ginkgo biloba is enhanced, which comprises fermenting Ginkgo biloba with acetobacter to obtain a fermented product of Acetobacter.
[10] The method according to [9] above, further comprising obtaining an extract of the fermented acetobacter.

The fermentation composition of the present invention can bring about an excellent blood flow promoting effect.

Hereinafter, the present invention will be described in detail.

The present invention relates to a fermentation composition derived from Ginkgo biloba, particularly a fermentation composition containing an acetobacter fermented product obtained by fermenting Ginkgo biloba with acetobacter or an extract thereof, and its use.

The ginkgo leaf used as a raw material in the present invention is a ginkgo (Ginkgo biloba) leaf. The ginkgo tree is not particularly limited, and may be a ginkgo tree produced in any production area, for example, from China or Japan. Ginkgo biloba may include all or part of the leaf.

In the present invention, Ginkgo biloba is fermented (oxidatively fermented) by acetobacter. The acetobacter used in the present invention is not particularly limited, but is preferably a bacterium belonging to the genus Acetobacter or the genus Gluconobacter. Examples of Acetobacter acetic bacteria include Acetobacter orleanensis, Acetobacter aceti, Acetobacter pasteurianus, Acetobacter estunensis, and Acetobacter estunensis. Examples include, but are not limited to, Acetobacter lovaniensis and Acetobacter orientalis. Gluconobacter acetic acid bacteria include Gluconobacter frateurii, Gluconobacter oxydans, Gluconobacter albidus, and Gluconobacter cerinus. , Gluconobacter asaii and the like, but are not limited thereto. One or more acetobacters may be used for fermentation of Ginkgo biloba in the present invention.

The ginkgo biloba to be fermented may be in any form suitable for fermentation, and may be, for example, a crushed product such as a coarsely crushed product or a finely crushed product, or a processed product such as leaf kneading or pressing. It may be untreated or untreated leaves. In one embodiment, the ginkgo biloba used for fermentation may be dried, for example, crushed after drying.

Ginkgo biloba is preferably sterilized before being used for fermentation. Ginkgo biloba can be sterilized by any sterilization method such as heat sterilization, filter sterilization, ultraviolet sterilization, radiation sterilization, and ozone sterilization. Ginkgo biloba may be sterilized by heating at more than 100 ° C. or 120 ° C. or higher, for example, 110 to 130 ° C. or 120 to 130 ° C., and in one example, it may be sterilized by treating at 121 ° C. for 20 minutes. After heat sterilization, it is preferable to use it for fermentation after cooling it to at least a temperature suitable for fermentation.

Fermentation of Ginkgo biloba with acetic acid bacteria can be carried out using any fermentation method suitable for acetic acid bacteria. In one embodiment, the fermentation of Ginkgo biloba with acetic acid bacteria is preferably carried out by a liquid culture method. Fermentation by the liquid culture method may be carried out using any aqueous medium, but can be carried out using any liquid medium suitable for culturing acetobacter.

Fermentation of Ginkgo biloba with acetic acid bacteria can be carried out by inoculating ginkgo biloba, which is a substrate for fermentation, with acetobacter and culturing it to ferment the ginkgo biloba. In one embodiment, acetobacter may be added to ginkgo biloba to which an aqueous medium such as water or liquid medium has been added, or acetobacter has been added to ginkgo biloba to which an aqueous medium such as water or liquid medium has not been added. May be good. An aqueous medium such as water or a liquid medium may be added in an appropriate amount to the ginkgo biloba, and the weight should be 0.01 to 50 times, 1 to 10 times, for example, 2 to 5 times the weight of the ginkgo biloba. It may be, but it is not limited to the amount. As the liquid medium, any liquid medium suitable for culturing acetic acid bacteria can be used. Ginkgo biloba to which an aqueous medium such as water or a liquid medium has been added may be sterilized as described above and used for fermentation, or an aqueous medium such as sterilized water or a liquid medium may be added to the sterilized ginkgo biloba.

Acetobacter may be directly added (inoculated) to Ginkgo biloba leaves without pre-culture, or liquid culture obtained by pre-culture may be added (inoculated). The efficiency of fermentation by acetobacter can be improved by sufficiently growing the acetic acid bacterium by preculture and then adding it to the ginkgo biloba as a liquid culture. The liquid culture of acetic acid bacteria may be one in which the acetic acid bacteria are cultured (precultured) in an arbitrary liquid medium suitable for culturing the acetic acid bacteria.

The liquid medium suitable for culturing acetic acid bacteria is not particularly limited, but usually contains a carbon source, a nitrogen source, and inorganic salts, and includes, for example, glucose, yeast extract, peptone, casein, soybean decomposition product, ethanol, glycerol, and potato. It may contain an appropriate ingredient selected from extracts and the like. In one embodiment, the liquid medium suitable for culturing acetic acid bacteria may be a liquid medium containing glucose, yeast extract, and inorganic salts.

Fermentation conditions of Ginkgo biloba with acetic acid bacteria are not particularly limited as long as the acetic acid bacteria can grow. The fermentation temperature may be any temperature at which acetobacter can grow and is not particularly limited, but is typically 15 to 45 ° C, preferably 20 to 40 ° C, for example 22 to 28 ° C. The pH at the time of fermentation may be any pH as long as acetobacter can grow, and is not particularly limited, but may be 3.0 to 8.0, preferably 4.0 to 7.0, for example 5.0 to 7.0. Since acetobacter produces organic acids during fermentation, the pH of the medium decreases as fermentation progresses. Therefore, the pH should be within the above range, preferably within the pH range of 4.0 to 7.0, for example, 5.0 to 7.0. It is preferable to carry out fermentation while adjusting the value. The fermentation time is not particularly limited, but may be, for example, 5 hours to 1 month, for example 1 to 10 days or 3 to 7 days. Other fermentation conditions such as the stirring speed of the medium may be conditions suitable for the growth of acetic acid bacteria.

In the present invention, a fermented acetic acid bacterium of Ginkgo biloba can be prepared as described above. The present invention provides a fermentation composition containing such a fermented product of Acetobacter in Ginkgo biloba. The fermented acetic acid bacteria of ginkgo leaves may be used as a fermentation composition as it is (fermentation stock solution when obtained by liquid culture), or concentrated, diluted, solvent exchanged, dried, sterilized / sterilized, and heated. , Cooling and / or deodorization / decolorization, or filtration for removal of impurities, cells and insoluble matter (for example, precision filtration) and / or sample clarification (purification) by centrifugation, etc. The one treated with the above may be used as a fermentation composition, and in either case, it is included in the range of "fermentation composition containing an acetate bacterium fermented product of ginkgo biloba".

In one embodiment, an extract (acetic acid bacterium fermented ginkgo biloba extract) may be prepared from the acetic acid bacterium fermented product of ginkgo biloba prepared as described above. The present invention also provides a fermentation composition containing an extract of a fermented acetobacter of Ginkgo biloba. An extract of the fermented acetobacter of Ginkgo biloba can be obtained by any extraction method. Extraction of Ginkgo biloba from the fermented acetic acid bacterium may be carried out by allowing the above-mentioned fermented acetic acid bacterium fermented product of Ginkgo biloba or a processed product thereof to stand, stir or shake. In this case, extraction is performed using water such as a liquid medium contained in the fermented acetobacter of Ginkgo biloba as a solvent. Alternatively, the extraction of Ginkgo biloba from the fermented acetic acid bacterium may be carried out by further adding a solvent to the above-mentioned fermented acetic acid bacterium fermented product of Ginkgo biloba or a processed product thereof, and allowing it to stand, stirring or shaking. ..

The amount of the solvent added for extraction to the above-mentioned fermented product of Ginkgo biloba or a processed product thereof is not limited to the following, but is usually 0.1 to 10 times the amount of the raw material Ginkgo biloba (dry weight), preferably 0.1 to 10 times. May be 0.7 to 8 times the amount, for example 2 to 6 times the amount (weight ratio). Alternatively, the amount of the solvent added for extraction to the above-mentioned fermented acetic acid bacterium of ginkgo biloba or its processed product is not limited to the following, but the amount of water contained in the fermented acetic acid bacterium or its processed product to be extracted is not limited to the following. , Usually 0.1 to 3 times, preferably 0.25 to 2.4 times, more preferably almost equal (0.8 to 1.2 times) (weight ratio). The total amount of the solvent for extraction (the total amount of the solvent added and the amount of water contained in the fermented acetic acid bacterium used for extraction or the processed product thereof) is not limited to the following, but the raw material ginkgo biloba ( The dry weight) may be 0.1 to 100 times, preferably 0.2 to 50 times, more preferably 1 to 20 times, for example, 2 to 10 times or 4 to 8 times (weight ratio).

The solvent for extraction (extraction solvent) is not particularly limited, but a solvent containing alcohol and / or water is preferable, and for example, water, alcohol, or a mixed solvent of water and alcohol (hydrous alcohol) may be used. .. Alternatively, the solvent for extraction may be water such as a liquid medium contained in the fermented acetobacter of Ginkgo biloba, or may be added with alcohol and / or water or the like. Those solvents are also included in the range of "solvents containing alcohol and / or water". The water may be any water such as pure water, ultrapure water, desalted water, sterilized water, distilled water, and tap water. The solvent containing water may be a buffer solution, a physiological saline solution, a dilute acid, a dilute alkali or the like. Examples of the alcohol include monohydric alcohols and polyhydric alcohols (for example, dihydric alcohols or trihydric or higher alcohols). Examples of the monohydric alcohol include ethanol, methanol, propyl alcohol, butyl alcohol, isobutyl alcohol, benzyl alcohol and the like. Examples of the polyhydric alcohol include 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin and the like. In one embodiment, the solvent is preferably a pharmaceutically acceptable solvent in the field of pharmaceuticals or cosmetics, preferably including, for example, a pharmaceutically acceptable alcohol or water in the field of pharmaceuticals or cosmetics. Alternatively, when the solvent is changed by solvent substitution or the like after extraction, a solvent other than that which is pharmaceutically acceptable in the field of pharmaceuticals or cosmetics may be used for extraction. More preferably, the solvent comprises water, alcohol, or hydrous alcohol (mixed solvent of water and alcohol).

In one embodiment, it is preferable to use a solvent having an alcohol concentration of 10 to 90 w / w%, more preferably 20 to 70 w / w%, for example 40 to 60 w / w%, for extraction, for example 20 to 70 w / w%. Alternatively, it is preferable to use a solvent containing 1,3-butylene glycol or ethanol having a concentration of 40 to 60 w / w% for extraction.

Extraction of Ginkgo biloba from the fermented acetic acid bacterium with a solvent is not limited to the following, but may be usually carried out at an extraction temperature of 10 to 80 ° C., preferably 20 to 60 ° C., 20 to 40 ° C., or room temperature. .. Extraction of Ginkgo biloba from the fermented acetic acid bacterium can be carried out, for example, at an extraction temperature of 10 to 80 ° C. for about 1 hour to 6 weeks, for example, an extraction time of 1 hour to 3 weeks or 5 to 24 hours. In one embodiment, the extraction of Ginkgo biloba from the acetic acid bacterium fermented product may be carried out at an extraction temperature of 20 to 60 ° C. for an extraction time of 1 hour to 3 weeks or 5 to 24 hours. In a preferred example, the extraction of Ginkgo biloba from the acetic acid bacterium fermented product may be carried out at an extraction temperature of 20 to 40 ° C. for 1 hour to 1 week, for example, an extraction time of 5 to 24 hours. In one embodiment, when the solvent contains ethanol (eg, an aqueous ethanol solution with a concentration of 20-70 w / w%), the extraction temperature is 20-60 ° C, eg 20-40 ° C, for 1 hour-3 weeks, eg 5-24. Extraction may be carried out over an time extraction time, or may be carried out at an extraction temperature of 20 to 40 ° C. for an extraction time of 1 hour to 1 week, for example, overnight (typically about 8 to 18 hours). .. In another embodiment, if the solvent contains 1,3-butylene glycol (eg, an aqueous solution of 1,3-butylene glycol at a concentration of 20-70 w / w%), extraction at 10-80 ° C, eg 20-40 ° C. Extraction may be carried out at a temperature of 1 hour to 3 weeks, for example 5 to 24 hours, and at an extraction temperature of 20 to 60 ° C. for 1 hour to 2 weeks, for example overnight (typically 8). Extraction may be performed for ~ 18 hours).

The extract of the fermented acetic acid bacterium of Ginkgo biloba in the present invention is an extract of the above-mentioned solvent obtained by the above-mentioned extraction method. The extract with a water-based solvent is an aqueous extract, the extract with an alcohol-based solvent is an alcoholic extract, and the extract with a hydrous alcohol-based solvent is an aqueous-alcoholic extract.

The extract of the acetic acid bacterium fermented product of ginkgo biloba obtained as described above may be used as it is (extract stock solution) as a fermentation composition, or concentrated, diluted, solvent exchanged, dried, sterilized and removed. It may be used as a fermentation composition after one or more treatments such as fungus, deodorization / decolorization, or purification by filtration (for example, precision filtration), centrifugation or the like. The purification method is not particularly limited, and for example, purification may be performed by filtering using a filter such as a membrane filter.

In a preferred embodiment, the obtained ginkgo biloba fermented acetic acid bacterium extract may be filtered, for example, filtered through a filter to remove impurities, bacterial cells, insoluble matter and the like. In one embodiment, the fermented acetic acid bacterium of ginkgo biloba is sterilized and then extracted as it is or by adding a solvent and allowing to stand, stir or shake, and the obtained extract is obtained. By filtering (for example, microfiltration), it is possible to obtain a filtered solution from which contaminants, bacterial cells, insoluble matter and the like have been removed.

In the present invention, the fermentation product of Ginkgo biloba or an extract thereof can be filtered by any filtration method. Filtration may be performed using a filter or other filtration device suitable for removing contaminants, bacterial cells and / or insoluble matter. Passage using a filter or other filtration device may be centrifugal filtration, pressure filtration, suction filtration and the like. The filter used for filtering the fermented acetic acid bacterium of Ginkgo biloba or an extract thereof may be any filter suitable for filtration. The filter is not limited to the following, but may be, for example, a membrane filter, a glass fiber filter, a cellulose filter, a sintered filter, or the like, or a combination of two or more types of filters. The filter may be, but is not limited to, a microfiltration filter, typically less than 1 μm, preferably 0.5 μm or less, more preferably 0.1 μm to 0.5 μm or 0.2 μm to 0.5 μm, eg 0.45. It may be a filter having a pore size of μm, 0.22 μm, or 0.1 μm. In one embodiment, filtration by a microfiltration filter may be performed after filtration by a pre-filter.

In the present invention, the fermentation product of Ginkgo biloba or an extract thereof can be diluted by any dilution method. In one embodiment, the fermented acetic acid bacterium of Ginkgo biloba or an extract thereof may be diluted with, for example, the above-mentioned solvent for extraction.

In the present invention, the concentration and solvent exchange of the fermented acetic acid bacterium of Ginkgo biloba or its extract can be carried out by any concentration method or solvent exchange method, for example, ultrafiltration method, dialysis method, chromatography method and the like. Can be done by.

In the present invention, the sterilization / sterilization of the acetic acid bacterium fermented product of ginkgo leaves or its extract is an arbitrary sterilization or sterilization method, for example, heat sterilization, filter sterilization, heat sterilization, ultraviolet sterilization, radiation sterilization, ozone sterilization. Such a method can be used, and a person skilled in the art can appropriately select a sterilization or sterilization method.

In the present invention, the deodorization / decolorization of the fermented acetic acid bacterium of Ginkgo biloba or its extract may be carried out by any deodorization treatment and / or decolorization treatment method, and examples thereof include a method using an activated carbon column or the like.

In the present invention, the fermented acetic acid bacterium of Ginkgo biloba or its extract can be dried by any drying method, for example, by a freeze-drying method such as a vacuum freeze-dried product or a heat-drying method such as a spray-drying method. It can be carried out.

In the present invention, a wintering treatment may be carried out on the fermented acetic acid bacterium of Ginkgo biloba or an extract thereof.

The fermented acetic acid bacterium of Ginkgo biloba or an extract thereof obtained as described above, or a fermentation composition containing the same, has an excellent blood flow promoting effect. The fermented acetic acid bacterium of Ginkgo biloba according to the present invention exhibits a significantly enhanced blood flow promoting effect as compared with unfermented Ginkgo biloba, as shown in Examples described later. It is preferable that the fermented acetic acid bacterium of Ginkgo biloba according to the present invention also exhibits a significantly enhanced blood flow promoting effect as compared with a fermented product of other fermenting microorganisms, particularly fungi such as yeast.

Therefore, the present invention provides a blood flow promoter containing a fermentation composition containing an acetic acid bacterium fermented product of Ginkgo biloba or an extract thereof according to the present invention. The blood flow promoter of the present invention is not particularly limited, but can bring about blood flow promotion when applied to the skin of a living body. In the present invention, "promoting blood flow" means that the blood flow after applying the fermentation composition containing the acetic acid bacterium fermented product of the ginkgo biloba according to the present invention or an extract thereof does not apply the fermentation composition (for example,). It refers to an increase in blood flow compared to the blood flow of a control group) to which water or a solvent is applied instead of the fermentation composition. Regarding the blood flow promoting effect of the fermentation composition containing the acetic acid bacterium fermented product of Ginkgo biloba or an extract thereof according to the present invention, the blood flow rate after applying the fermentation composition is preferably compared with the blood flow rate of the control group. It can be increased by 20% or more, preferably 100% or more, more preferably 200% or more.

In the present invention, the blood flow promoting action of Ginkgo biloba can be enhanced by fermenting Ginkgo biloba with acetic acid bacteria as described above. Therefore, the present invention also provides a method for producing a fermentation composition in which the blood flow promoting action of Ginkgo biloba is enhanced, which comprises fermenting Ginkgo biloba with acetobacter to obtain an acetobacter fermented product as described above. To do. The method may further include obtaining an extract of the fermented acetic acid bacterium as described above.

The fermentation composition containing the fermented acetic acid bacterium of Ginkgo biloba or an extract thereof according to the present invention, or a blood flow promoter containing the same may be used orally or parenterally, but is particularly advantageous as an external preparation for skin. Can be used. The fermented composition containing the acetic acid bacterium fermented product of Ginkgo biloba or an extract thereof according to the present invention can promote the blood flow of the skin and increase the skin temperature by applying it to the skin.

The fermentation composition containing the fermented acetic acid bacterium of Ginkgo biloba or an extract thereof according to the present invention, or the blood flow promoter containing the same can be used, for example, in combination with cosmetics. That is, the present invention also provides a fermentation composition containing an acetobacter fermented product of Ginkgo biloba or an extract thereof according to the present invention, or a cosmetic containing a blood flow promoter containing the same. The cosmetic according to the present invention can bring about a blood flow promoting effect when applied to the skin. Therefore, the present invention also provides cosmetics for promoting blood flow. The cosmetic according to the present invention may be an external preparation for skin.

The fermentation composition containing the fermented acetic acid bacterium of Ginkgo biloba or an extract thereof according to the present invention, or the blood flow promoter containing the same can be used, for example, by blending it in a medicine. That is, the present invention also provides a fermentation composition containing an acetobacter fermented product of Ginkgo biloba or an extract thereof according to the present invention, or a medicine containing a blood flow promoter containing the same. The medicament according to the present invention can bring about a blood flow promoting effect when applied to the skin. Therefore, the present invention also provides a medicine for promoting blood flow. The medicine according to the present invention may be, for example, a medicine or a quasi-drug. The medicament according to the present invention may be an oral drug or a parenteral drug, but is preferably an external preparation, and more preferably a skin external preparation. The medicament according to the present invention may be of any dosage form, and may be, for example, an ointment, a patch, a pap, a liniment, a lotion, a cream, an extract, a flow extract, a tincture, an elixir, or a syrup. Examples thereof include agents, limonade agents, aromatic water agents, liquid agents, suspending agents, emulsions, medicated bathing agents, medicated shampoos, aerosol agents, powders, granules, tablets, capsules and the like.

The cosmetics and medicines according to the present invention may be compositions in any form such as solution, suspension, emulsified, powder, paste, mousse, or gel. The cosmetics and medicines according to the present invention may preferably be in any form that can be used in contact with the skin, and specifically, for example, lotions, milky lotions, beauty liquids, general creams (face creams, etc.). Hand cream, body cream, etc.), wash pigment (cleansing cream, etc.), pack, shaving cream, sunscreen cream, sunscreen cream, sunscreen lotion, sunscreen lotion, cosmetic soap, foundation, whitewash, powder, lipstick, lip cream , Eye liner, eye cream, eye shadow, mascara, bathing agent, shampoo, hair rinse, hair treatment, hair pack, scalp care agent, body rinse, body gel, hair dye, cosmetics for hair and the like. Hand creams, eye creams, shampoos, hair packs, scalp care agents and the like are particularly preferred.

A fermented composition containing an acetic acid bacterium fermented product of Ginkgo biloba or an extract thereof according to the present invention, or a blood flow promoter containing the same, can also be applied to skin that tends to generate an undesired odor, such as the scalp, to cause odor. It is also possible to suppress the occurrence. Therefore, the fermentation composition containing the acetic acid bacterium fermented product of ginkgo biloba or an extract thereof according to the present invention, or the blood flow promoter containing the fermented product, is an active ingredient of cosmetics or pharmaceuticals applied to skin that tends to generate odor such as scalp. For example, it can be suitably used in shampoos, conditioners, hair treatments, hair packs, scalp care agents, hair dyes, hair cosmetics and the like.

The amount of the fermentation composition containing the acetic acid bacterium fermented product of the ginkgo biloba according to the present invention or an extract thereof, or the blood flow promoter containing the same, is the amount of the ginkgo biloba according to the present invention in the composition of cosmetics or pharmaceuticals. The dry weight of the fermented acetic acid bacterium or its extract is 0.0001 to 100 w / w%, preferably 0.001 to 100 w / w%, more preferably 0.01 to 30 w / w%, and more preferably 0.1 to 10 w / w%. It may be an amount that becomes the concentration of. The fermentation composition containing the acetic acid bacterium fermented product of the ginkgo biloba according to the present invention or an extract thereof, or the blood flow promoter containing the same, can be blended in any form with the cosmetic or medicine according to the present invention. For example, it may be simply mixed with other components as it is, or may be blended in cosmetics or pharmaceuticals in the form of liquid, gel, powder, granule, capsule inclusion body or the like.

The cosmetic according to the present invention is a fermentation composition containing an acetobacter fermented product of ginkgo biloba or an extract thereof according to the present invention, or a blood flow promoter containing the same, and an additive permitted in cosmetics. And, if necessary, other active ingredients and the like can be contained. As such additives and other active ingredients, those suitable for the use and form of the cosmetic can be arbitrarily selected and used. The cosmetic according to the present invention can be produced according to any cosmetic production method, for example, by mixing, stirring, molding, filling or the like of raw materials.

The medicament according to the present invention is a fermentation composition containing a fermented acetic acid bacterium of Ginkgo biloba or an extract thereof according to the present invention, or a blood flow promoter containing the same, as well as a pharmaceutically acceptable additive and necessary. Other active ingredients and the like can be included depending on the above. As such additives and other active ingredients, those suitable for the purpose and form of the pharmaceutical can be arbitrarily selected and used. The pharmaceutical product according to the present invention can be produced by mixing, stirring, molding, filling and the like of raw materials according to an arbitrary pharmaceutical production method.

The blood flow promoter, cosmetics and pharmaceuticals according to the present invention include carriers (solid or liquid carriers), excipients, surfactants, binders, disintegrants, lubricants, odorants, solubilizers and suspensions. It may contain one or more pharmaceutical additives such as agents, coating agents, pH regulators, colorants, fragrances, flavoring agents, refreshing agents, stabilizers, foaming agents, preservatives, buffers and the like.

The blood flow promoter, cosmetics and medicine according to the present invention may contain, for example, one or more components arbitrarily selected from any of the following groups.

Proteins such as collagen, collagen hydrolyzate, gelatin, gelatin hydrolyzate, elastin, elastin hydrolyzate, lactoferrin, keratin, keratin hydrolyzate, casein, albumin, royal jelly-derived protein hydrolyzate, honey-derived protein hydrolyzate, etc. And protein hydrolysates.

Natural amounts of sodium hyaluronate and hyaluronic acid derivatives, xanthan gum, carrageenan, guar gum, alginic acid and its salts, pectin, chondroitin sulfate and its salts, water-soluble chitin, chitosan derivatives and their salts, pullulan, deoxyribonucleic acid, arabic rubber, tragant rubber, etc. Molecules and their derivatives.

Fruit-derived products such as aloe, apples, mandarins, thighs, mandarins, melons, strawberries, seek arthurs, figs, seed-derived products such as peanuts, chestnuts, ryugan, millet, barley, rice, goyomatsu, soybeans, Japanese pear, ostrich fern ( Leaf-derived products such as young shoots of apple), rhizome-derived products such as wasabi, and plant-derived products such as cherry blossoms and mandarin oranges.

Humus humic acid and fulvic acid, which are the final products obtained by decomposing plants by microorganisms.

Crude drugs such as physalis, luo han guo, jujube, adlay, licorice, tochu, ginger, and udo.

Seaweed powder, seaweed extract, and seaweed-derived polysaccharides.

Animal-derived products such as placenta extract.

Water such as carbonated water, hot spring water, micro-nano bubble water, purified water, distilled water, etc.

Among acidic polymers such as carboxyvinyl polymer and its salt, polyacrylic acid and its salt, carboxymethyl cellulose and its salt, polyvinyl alcohol, hydroxyethyl cellulose, hydroxypropyl cellulose, methyl cellulose, polyethylene glycol, polyvinyl pyrrolidone, nitrocellulose polyvinyl methyl ether and the like. Sex polymer.

Cationic polymers such as cationized cellulose, polyethyleneimine, and cationized guar gum.

Lower alcohols such as ethanol and isopropyl alcohol.

UV absorbers such as para-aminobenzoic acid-based UV absorbers, salicylic acid-based UV absorbers, cinnamic acid-based UV absorbers, anthranieric acid-based UV absorbers, and benzophenone-based UV absorbers.

Vitamin A, B vitamins, vitamin C, L-ascorvir magnesium phosphate and its derivatives, vitamin D group, d-α-tocophenol acetate, vitamin E group, folic acids, β-carotene, γ-orizanol, nicotinic acid, Vitamins such as pantothenic acids, biotins, and ferulic acid.

Anti-inflammatory agents such as glycyrrhizic acid and its salts, guaiazulene and its derivatives, and allantoin.

Antioxidants such as stearic acid ester, nordihydroguaceretenic acid, dibutylhydroxytoluene, butylhydroxyanisole, parahydroxyanisole, propyl gallate, sesamol, sesamolin, and gossypol.

Preservatives such as parabenzoic acid esters such as methyl parabenzoate, ethyl parabenzoate, propyl parabenzoate, and butyl parabenzoate, sorbic acid, dehydroacetic acid, phenoxyethanol, and benzoic acid.

Edetoic acid such as edetic acid and disodium edetate and salts thereof, and sequestrants such as phytic acid and hydroxyethanedisulfonic acid.

Multivalent alcohols such as glycerin, 1,3-butylene glycol and propylene glycol.

Amino acids such as L-aspartic acid, DL-alanine, L-arginine, L-cysteine, L-glutamic acid, and glycine, and salts thereof.

Sugars such as maltitol, sorbitol, xylobiose, N-acetyl-D-glucosamine, and honey.

Bases such as aqueous ammonia, monoethanolamine, triethanolamine, sodium hydroxide, and potassium hydroxide.

Hydrocarbons such as liquid paraffin, squalane, and petrolatum.

Oils and fats such as olive oil, palm oil, evening primrose oil, jojoba oil, castor oil, and hardened castor oil.

Fatty acids such as lauric acid, myristyl acid, palmitic acid, stearic acid, and behenic acid.

Higher alcohols such as myristyl alcohol, cetanol, cetostearyl alcohol, stearyl alcohol, and behenyl alcohol.

Esters such as isopropyl myristate, isopropyl palmitate, cetyl octanoate, glycerin trioctanoate, octyldodecyl myristate, octyl stearate, and stearyl stearate.

Phospholipids such as lecithin and its derivatives.

Animal and plant-derived lipids such as bovine bone marrow fat and bovine brain lipids.

Alkyl sulfates such as ammonium lauryl sulfate, ethanolamine lauryl sulfate, sodium lauryl sulfate, and triethanolamine lauryl sulfate.

Polyoxyethylene (2EO) Lauryl Ether Triethanolamine Sulfate (EO is ethylene oxide, and the value before EO indicates the number of moles of ethylene oxide added;), Polyoxyethylene (3EO) alkyl (11 to 15 carbon atoms) Any one or a mixture of two or more) Polyoxyethylene alkyl sulfate such as ether sodium sulfate.

Alkylbenzene sulfonates such as sodium laurylbenzene sulfonate and triethanolamine laurylbenzene sulfonate.

Polyoxyethylene alkyl ether sulfates such as polyoxyethylene (3EO) tridecyl ether sodium acetate.

Palm oil fatty acid sarcosin sodium, lauroyl sarcosin triethanolamine, lauroyl methyl-L-sodium glutamate, coconut oil fatty acid-L-glutamate sodium, coconut oil fatty acid-L-glutamate triethanolamine, coconut oil fatty acid methyl taurine sodium, lauroyl methyl taurine N-acylamino acid salts such as sodium, sodium alkane sulfonate ether sulfate, sodium glycerin sulfate of hardened coconut oil fatty acid, sodium glycerin sulfate of hardened coconut oil, disodium undecinoylamide ethyl sulfosuccinate, sodium octylphenoxydientoxyethyl sulfonate , Amino sulfosuccinate disodium oleate, dioctyl sulfosuccinate, dioctyl sodium sulfosuccinate, disodium lauryl sulfosuccinate, polyoxyethylene alkyl (carbon chains 12-15) ether phosphate (8-10EO), polyoxyethylene oleyl ether Anionic surfactants such as sodium phosphate, sodium polyoxyethylene cetyl ether phosphate, disodium lauryl lauryl polyoxyethylene sulfosuccinate, sodium polyoxyethylene lauryl ether phosphate, sodium lauryl sulfoacetate, and sodium tetradecene sulfonate.

Stearyldimethylammonium chloride, di (polyoxyethylene) oleylmethylammonium, stearyldimethylbenzylammonium chloride, stearyltrimethylammonium chloride, tri (polyoxyethylene) stearylammonium chloride, polyoxypropylene methyldiethylammonium chloride, myristyldimethylbenzylammonium chloride , Cationic surfactants such as lauryltrimethylammonium chloride.

2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine, undecyl hydroxyethyl imidazolinium betaine sodium, undecyl-N-hydroxyethyl-N-carboxymethyl imidazolinium betaine, stearyldihydroxyethyl betaine, palm Oil fatty acid amide propyl betaine, coconut oil alkyl-N-carboxyethyl-N-hydroxyethyl imidazolinium betaine sodium, coconut oil alkyl-N-carboxymethoxyethyl-N-carboxymethyl imidazolinium disodium lauryl sulfate, N-palm An amphoteric surfactant such as the oil fatty acid acyl-L-arginine ethyl-DL-pyrrolidone carboxylate.

Polyoxyethylene alkyl (12-14 carbon atoms) ether (7EO), polyoxyethylene octylphenyl ether, polyoxyethylene oleyl ether, glycerin polyoxyethylene oleate, polyoxyethylene stearyl ether, polyoxyethylene cetyl ether, polyoxy Ethylenecetylstearyldiether, polyoxyethylene sorbitol lanolin (40EO), polyoxyethylene nonylphenyl ether, polyoxyethylene polyoxypropylene cetyl ether, polyoxyethylene polyoxypropylene decyltetradecyl ether, polyoxyethylene lanolin, polyoxy Nonionic surfactants such as ethylene lanolin alcohol and polyoxypropylene stearyl ether.

Isostearic acid diethanolamide, undecylene acid monoethanolamide, oleic acid diethanolamide, beef fatty acid monoethanolamide, hardened beef fatty acid diethanolamide, stearic acid diethanolamide, stearic acid diethylaminoethylamide, stearic acid monoethanolamide, myristate diethanolamide, Thickeners such as coconut oil fatty acid diethanolamide, coconut oil fatty acid ethanolamide, lauric acid isopropanolamide, lauric acid ethanolamide, lauric acid diethanolamide, and lanolin fatty acid diethanolamide.

Silicone oils such as chain or cyclic methylpolysiloxane, methylphenylpolysiloxane, dimethylpolysiloxane polyethylene glycol copolymer, dimethylpolysiloxane polypropylene copolymer, amino-modified silicone oil, and quaternary ammonium-modified silicone oil.

Thioglycolic acid and its salts.

Cysteamine and its salts.

Peroxides such as hydrogen peroxide, persulfates, borates and urea peroxides.

Bromates such as sodium bromate and potassium bromate.

Other active ingredients such as keratolytic agents, astringents, wound healing agents, oral preparations, deodorant / deodorants, antiallergic agents, cell activators, etc.

The blood flow promoter according to the present invention, or the application or administration target of the cosmetic or pharmaceutical according to the present invention is an object (subject) of any mammal including humans, livestock, pet animals, laboratory animals, and the like. Well, especially humans are preferred. The blood flow promoter according to the present invention, or the cosmetic or pharmaceutical according to the present invention, is used for subjects in which blood flow is decreased, the risk of blood flow decrease (for example, genetic factors (predisposition), environment, constitution, age, etc.). It is particularly useful for application or administration to subjects with high risk) and subjects who can be expected to have health or cosmetic benefits by promoting blood flow. The blood flow promoter according to the present invention, or the cosmetic or pharmaceutical according to the present invention, is also applied or administered to a subject who is likely to (frequently occurs) or has a high risk of causing skin odor such as scalp. It is useful.

The blood flow promoter according to the present invention, or the cosmetic or pharmaceutical according to the present invention, is used to promote blood flow, preferably to promote blood flow in the skin, or to bring health or cosmetic benefits thereof. be able to. In one embodiment, the blood flow promoter according to the present invention, or the cosmetic or pharmaceutical according to the present invention, is used for improving, for example, thinning hair, plucking, dark circles, etc. (an effect can be expected by promoting blood flow). Can be used. The blood flow promoter according to the present invention, or the cosmetic or pharmaceutical according to the present invention can also be used to suppress the odor of the skin such as the scalp.

The applicable amount or dose of the blood flow promoter according to the present invention or the cosmetic or pharmaceutical according to the present invention varies depending on the age, body weight, application / administration route, dosage form, number of administrations, etc. of the subject, and is at the discretion of those skilled in the art. Can be changed extensively by. Specifically, the applied amount or dose thereof is not particularly limited, but is converted into the dry weight of the acetic acid bacterium fermented product of the ginkgo biloba according to the present invention, which is the active ingredient, or an extract thereof, for example, 0.001 mg / mg / dose. It can be from 10g / time. The blood flow promoter according to the present invention, or the cosmetic or pharmaceutical according to the present invention is not limited to the following, and may be repeatedly applied or administered, for example, at intervals of 6 to 24 hours.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the technical scope of the present invention is not limited to these examples.

[Example 1] Preparation of fermented or unfermented ginkgo biloba extract <Production Example 1>
Ginkgo biloba (150 g) crushed after drying was sterilized at 121 ° C. for 20 minutes by adding 3 times the weight of water (450 g). After cooling to 30 ° C, a liquid culture (about 150 g) of Acetobacter orleanensis, a type of acetobacter that was pre-cultured using a liquid medium containing glucose, yeast extract, and inorganic salts as nutritional components, was added. Then, the ginkgo biloba was fermented at 25 ° C. for 5 days while continuously adjusting the pH to 6.5 with an aqueous sodium hydroxide solution. After the completion of fermentation for 5 days, the fermented product was sterilized by heating at 121 ° C. for 20 minutes. After cooling to room temperature, extraction was performed by stirring overnight under room temperature conditions. The obtained crude extract containing the solid content was filtered through a pre-filter and then a 0.45 μm membrane filter to obtain a filtered solution from which bacterial cells and insoluble solids had been removed. After measuring the dry weight of the filtrate, the mixture was diluted with water so as to have a dry weight of 1 w / w% to prepare an acetobacter fermented ginkgo biloba extract.

<Manufacturing example 2>
In the same manner as in Production Example 1, dried and crushed ginkgo biloba (150 g) was fermented, sterilized by heating, and cooled to obtain a sterilized fermented product. Extraction was carried out by adding 100% 1,3-butylene glycol having the same weight (about 600 g) as the water content in the obtained sterilized fermented product and stirring overnight at room temperature. The obtained crude extract containing the solid content was filtered through a pre-filter and then a 0.45 μm membrane filter to obtain a filtered solution from which bacterial cells and insoluble solids had been removed. After measuring the dry weight of the filtrate, dilute it by adding 50% 1,3-butylene glycol aqueous solution (1,3-BG water) so that the dry weight is 1 w / w%, and acetobacter fermented ginkgo biloba leaves. A 50% BG water extract was prepared.

<Manufacturing example 3>
Ginkgo biloba (150 g) crushed after drying was sterilized at 121 ° C. for 20 minutes by adding 3 times the weight of water (450 g). After cooling to 30 ° C., 100% 1,3-butylene glycol of the same weight (about 450 g) as the water added to the ginkgo biloba was added, and the mixture was stirred overnight at room temperature for extraction. The obtained crude extract containing the solid content was filtered through a pre-filter and then a 0.45 μm membrane filter to obtain a filtered solution from which insoluble solids had been removed. After measuring the dry weight of the filtrate, a 50% 1,3-butylene glycol aqueous solution was added to dilute it so that the dry weight was 1 w / w%, and a 50% BG water extract of ginkgo biloba was prepared.

<Manufacturing example 4>
Ginkgo biloba (150 g) crushed after drying was sterilized at 121 ° C. for 20 minutes by adding 3 times the weight of water (450 g). After cooling to 30 ° C, a liquid culture (about 150 g) of Saccharomyces cerevisiae, a type of yeast pre-cultured using a liquid medium containing glucose, yeast extract, and inorganic salts as nutritional components, was added. Saccharomyces cerevisiae leaves were fermented at 25 ° C. for 5 days while continuously adjusting the pH to 6.5 with an aqueous solution of sodium hydroxide. After the completion of fermentation for 5 days, the mixture was sterilized by heating at 121 ° C. for 20 minutes and cooled to obtain a sterilized fermented product. Extraction was carried out by adding 100% 1,3-butylene glycol having the same weight (about 600 g) as the water content in the obtained sterilized fermented product and stirring overnight at room temperature. The obtained crude extract containing the solid content was filtered through a pre-filter and then a 0.45 μm membrane filter to obtain a filtered solution from which bacterial cells and insoluble solids had been removed. After measuring the dry weight of the filtrate, 50% 1,3-butylene glycol aqueous solution was added and diluted so that the dry weight was 1 w / w% to obtain a 50% BG water extract of Saccharomyces fermented ginkgo biloba. ..

<Manufacturing example 5>
In the same manner as in Production Example 1, dried and crushed ginkgo biloba (150 g) was fermented, sterilized by heating, and cooled to obtain a sterilized fermented product. Extraction was carried out by adding absolute ethanol having the same weight (about 600 g) as the water content in the obtained sterilized fermented product and stirring overnight at room temperature. The obtained crude extract containing the solid content was filtered through a pre-filter and then a 0.45 μm membrane filter to obtain a filtered solution from which bacterial cells and insoluble solids had been removed. After measuring the dry weight of the filtrate, a 50% ethanol aqueous solution was added to dilute it so that the dry weight was 1 w / w%, and an acetobacter fermented ginkgo biloba 50% ethanol water extract was prepared.

<Manufacturing example 6>
Ginkgo biloba (150 g) crushed after drying was sterilized at 121 ° C. for 20 minutes by adding 3 times the weight of water (450 g). Liquid culture of Gluconobacter frateurii, a type of acetobacter, pre-cultured using a liquid medium containing glucose, yeast extract, and inorganic salts as nutritional components after cooling to 30 ° C (about 150 g) Was added, and the ginkgo biloba was fermented at 25 ° C. for 5 days while continuously adjusting the pH to 6.5 with an aqueous solution of sodium hydroxide. After the completion of fermentation for 5 days, the fermented product was sterilized by heating at 121 ° C. for 20 minutes. After cooling to room temperature, extraction was performed by stirring overnight under room temperature conditions. The obtained crude extract containing the solid content was filtered through a pre-filter and then a 0.45 μm membrane filter to obtain a filtered solution from which bacterial cells and insoluble solids had been removed. After measuring the dry weight of the filtrate, the mixture was diluted with water so as to have a dry weight of 1 w / w% to prepare a gluconobacter fermented ginkgo biloba extract.

[Example 2] Evaluation of blood flow promoting effect The blood flow promoting (blood flow improving) effect of the ginkgo biloba extract prepared in Example 1 was tested using 10 healthy adult men and women as subjects.

The subjects were acclimatized in a constant temperature and humidity room (room temperature 20 ° C., humidity 40 ° C.) for 15 minutes, and then the entire left palm was immersed in cold water at 10 ° C. for 1 minute. Moisture in the entire palm was quickly wiped off, a contact-type EG probe of a laser tissue blood flow meter was attached to the tip of the left ring finger, which was the test site, and the tissue blood flow was measured after 1 minute of rest. Next, the tip of the left ring finger, which is the test site, was immersed in the ginkgo biloba extract prepared as in Production Examples 1 to 6 of Example 1 in advance at 30 ° C. for 15 minutes, after which the water was wiped off and the probe was attached. The tissue blood flow was measured after 1 minute of resting. The blood flow improvement rate was calculated for each subject as the ratio (%) of the tissue blood flow after immersion in the Ginkgo biloba extract to the tissue blood flow after immersion in cold water. Similar measurements were performed using a 50% BG aqueous solution instead of the Ginkgo biloba extract as a control.

Table 1 shows the average of 10 subjects with a blood flow improvement rate.

No increase in blood flow was observed in the control group. On the other hand, in the test group immersed in the extracts prepared in Production Examples 1, 2, 3, 4, 5, and 6, an increase in blood flow was observed. In particular, the acetobacter-fermented ginkgo biloba extract prepared in Production Examples 1, 2, 5 and 6 is compared with the non-fermented Ginkgo biloba extract prepared in Production Example 3 and the yeast-fermented Ginkgo biloba extract prepared in Production Example 4. And increased blood flow more. Furthermore, the extract using 1,3-butylene glycol or ethanol tended to be more effective in increasing blood flow than the water extract.

From these results, by fermenting Ginkgo biloba with acetobacter, it is possible to enhance the blood flow promoting (blood flow improving) action of Ginkgo biloba, and also bring about the enhancement of the blood flow promoting (blood flow improving). It was shown that the components can be extracted from the fermented acetobacter of Ginkgo biloba using water or alcohol.

[Example 3] Evaluation of changes in skin temperature The changes in skin temperature after application of the ginkgo biloba extract prepared in Example 1 were examined using 10 healthy adult men and women as subjects.

After washing the inside of the forearm of the subject and acclimatizing for 30 minutes in a constant temperature and humidity chamber, the ginkgo biloba extract prepared as described in Production Examples 1, 2, 3 and 4 was placed in an area of about 2 × 2 cm inside the forearm. The non-woven fabric was impregnated and 0.5 g was applied. After application, it was left for 10 minutes and wiped off. The skin temperature before application and 30 minutes after wiping was measured by infrared thermography, and the amount of change in skin temperature after application with respect to the skin temperature before application was calculated for each subject. As a control, a group was provided in which distilled water was similarly applied instead of the ginkgo biloba extract.

Table 2 shows the average amount of change in skin temperature before and after application of each extract.

No temperature change was observed in the control group, whereas in the test group to which the acetobacter-fermented ginkgo biloba extract prepared as in Production Examples 1 and 2 was applied, the skin temperature increased significantly as compared with that before application. .. The Acetobacter-fermented ginkgo biloba 50% BG water extract of Production Example 2 further significantly increased the skin temperature as compared with the Acetobacter-fermented ginkgo biloba water extract of Production Example 1.

[Example 4] Shampoo production and evaluation of warmth effect According to the formulation shown in Table 3, components (1) to (9) were stirred at 70 ° C., dissolved, and then cooled to room temperature to produce a shampoo (test sample). .. As a comparative sample, a shampoo was produced by the same method as the test sample except that the gluconobacter fermented ginkgo biloba extract of component (5) was not blended.

These shampoos were subjected to a sensory test by 10 specialized panelists. In the sensory test, the following items were evaluated on a 5-point scale.

A) Warm feeling of the scalp after washing hair 1 Cold 2 Slightly cold 3 Normal 4 Slightly warm 5 Warm B) Scalp odor 1 day after washing hair 1 Smell 2 Slightly odor 3 Normal 4 Not so odor 5 Panelists who do not smell The average score is shown in Table 4.

As shown in Table 4, the shampoo of the test sample containing the gluconobacter fermented ginkgo biloba extract has an excellent warmth compared to the shampoo of the comparative sample (without the gluconobacter fermented ginkgo biloba extract). It showed an effect and an odor suppressing effect.

[Example 5] Production of hair rinse According to the formulation shown in Table 5, components (1) to (9) were mixed and stirred at 80 ° C. and dissolved to produce a hair rinse. When the hair was rinsed with the obtained hair rinse, the feel of the hair was smooth and the hair was moisturized.

[Example 6] Production of cream agent In accordance with the formulation shown in Table 6, components (1) to (7) are mixed and stirred at 80 ° C., and components (8) to (12) are separately added to the mixture at 80 ° C. The mixture obtained by mixing and stirring in the above was added, homogenized, and cooled to room temperature with stirring to produce a cream.

The obtained cream was not sticky during use and moisturized the skin.

[Example 7] Production of body gel agent A body gel agent was produced by stirring and dissolving the components (1) to (8) according to the formulation shown in Table 7.

The obtained body gel agent was not sticky during use and moisturized the skin.

[Example 8] Production of eye cream According to the formulation shown in Table 8, components (1) to (3) were stirred and dissolved to prepare a phase A. Ingredients (4) to (8) were dissolved by heating and added to Phase A with stirring for emulsification. After the temperature was lowered to room temperature, the component (9) was added and the mixture was thoroughly stirred.

The obtained eye cream was excellent in usability.

[Example 9] Production of lipstick According to the formulation shown in Table 9, the components (1) to (7), (10), (15), and (16) are mixed and stirred while heating at 60 ° C. to obtain phase A. Prepared. Separately, the components (8), (9), and (17) are mixed, the component (11) is kneaded therein, then the components (12) to (14) are kneaded, and then heated at 60 ° C. Then, the B phase was prepared. Phase A was added to phase B, and the mixture was mixed and stirred while heating at 60 ° C., poured into a shape, cooled, and solidified.

The obtained lipstick was excellent in usability.

Claims (10)

A fermentation composition containing a fermented product of acetic acid bacteria of Ginkgo biloba or an extract thereof. The fermentation composition according to claim 1, wherein the acetobacter belongs to the genus Acetobacter or the genus Gluconobacter. The fermentation composition according to claim 1 or 2, wherein the acetobacter is Acetobacter orleanensis or Gluconobacter frateurii. The fermentation composition according to any one of claims 1 to 3, wherein the extract is an extract from a solvent containing alcohol and / or water. The fermentation composition according to claim 4, wherein the alcohol is 1,3-butylene glycol or ethanol. A blood flow promoter comprising the fermentation composition according to any one of claims 1 to 5. A cosmetic for promoting blood flow, which comprises the fermentation composition according to any one of claims 1 to 5. A medicine for promoting blood flow, which comprises the fermentation composition according to any one of claims 1 to 5. A method for producing a fermentation composition in which the blood flow promoting action of Ginkgo biloba is enhanced, which comprises fermenting Ginkgo biloba with acetobacter to obtain a fermented product of Acetobacter. The method according to claim 9, further comprising obtaining an extract of the fermented acetic acid bacterium.