JP4916143B2 - Method for producing a composition having a collagen production promoting action from an alcoholic beverage fermented with brewer's yeast and the composition - Google Patents

Description

  The present invention relates to a method for producing a composition having a collagen production promoting action from an alcoholic beverage fermented with brewer's yeast and the composition.

  Alcoholic beverages fermented with brewer's yeast such as beer and sparkling liquors are representative low alcoholic beverages that are brewed 147 million kl annually worldwide (2003) and are loved by many people.

  Alcoholic beverages fermented with brewer's yeast such as beer and sparkling liquor are made from various grains such as malt, brewer's yeast, hops and water. Therefore, these alcoholic beverages contain a variety of nutritional components in a well-balanced manner, such as sugars, proteins, amino acids, vitamins, minerals, alcohols and hop extracts.

  So far, many cosmetics, bathing agents, quasi-drugs or pharmaceuticals using sake fermented with bran mold have been reported (Patent Documents 1 to 6), but beer is treated with activated carbon. Only a method for producing cosmetics is disclosed (Patent Document 7).

  On the other hand, it has been reported that proline has an action of promoting the production of collagen and keratin in hair and skin (Patent Documents 8 and 9).

JP-A-6-172124 JP-A-8-81350 JP-A-8-133960 JP-A-8-168378 JP 08-048612 A JP-A-9-110668 JP 2001-106610 A JP-A-10-087444 JP 2002-080321 A

  However, the method for producing cosmetics from the beer is intended only to remove aldehydes, ketones, organic acids and the like. The effects of cosmetics produced by this method are limited to a refreshing feeling, a moist feeling, a cleaning effect, and a moisturizing effect.

  It is known in the beer brewing industry that brewer's yeast does not use proline, which is a kind of amino acid in the fermentation process, and it is expected that alcoholic beverages fermented with brewer's yeast contain a relatively large amount of proline. So far, there has been no idea or attempt to produce a composition having a collagen production promoting action from an alcoholic beverage fermented with brewer's yeast.

  Therefore, the present invention intends to provide a method for producing a novel composition having a collagen production promoting action from an alcoholic beverage fermented with brewer's yeast.

  As a result of extensive research, the inventors of the present invention succeeded in producing a composition having a collagen production promoting action from beer, and this composition contains amino acids such as proline and oligosaccharides. I found. Furthermore, surprisingly, this composition has an anti-aging action, a moisturizing action and a whitening action in addition to a collagen production promoting action, and cosmetics containing this composition have a smoothing action and hair protection. As a result, the present invention has been completed.

That is, the present invention includes a first step of removing carbon dioxide gas, a second step of removing alcohol by vacuum concentration and insolubilizing polysaccharides and proteins, and a third step of removing polysaccharides and proteins. And a fourth step of purifying with an ion exchange resin, a method for producing a composition having a collagen production promoting action from an alcoholic beverage fermented with brewer's yeast. This method removes alcohol and polymer polysaccharides and proteins that irritate the skin from alcoholic beverages fermented with brewer's yeast, as well as amino acids such as proline having a collagen production promoting action and oligosaccharides having a moisturizing action. It is possible to extract active ingredients. The novel composition produced by this method has an anti-aging action, a moisturizing action and a whitening action in addition to the collagen production promoting action.

  The alcoholic beverage fermented with the brewer's yeast is preferably at least one alcoholic beverage selected from the group consisting of beer, happoshu and beer-like alcoholic beverages. Since these alcoholic beverages are made from a carbon source, nitrogen source, hops, brewer's yeast and water as raw materials and are produced by a similar production method, one or a mixture of two or more of these alcoholic beverages is used. Compositions manufactured according to the method of the present invention as raw materials are expected to have similar components including proline and oligosaccharides. Therefore, it is possible to produce a novel composition having an anti-aging effect, a moisturizing effect and a whitening effect in addition to the collagen production promoting effect from these alcoholic beverages.

  It is preferable that the 1st process of the method of this invention is performed by stirring of an alcoholic beverage. By stirring the alcoholic beverage, it is possible to generate and remove carbon dioxide without causing a chemical change.

  It is preferable that the second step and the third step of the method of the present invention are simultaneously performed by vacuum concentration. By reducing the pressure under reduced pressure, the alcohol can be evaporated and removed without chemical change, and at the same time, the high-molecular polysaccharides and proteins cannot be solubilized and removed as starch.

  The fourth step of the method of the present invention is preferably purification with an anion exchange resin and a cation exchange resin. By using both an anion exchange resin and a cation exchange resin, it is possible to completely remove the color component and scent component to be removed. In addition, amino acids such as proline and oligosaccharides are not removed by this step, and can be included in the composition of the present invention which is the final product.

  The present invention also provides a composition having a collagen production-promoting action obtained by the above method from an alcoholic beverage fermented with brewer's yeast. This composition contains proline and oligosaccharide, and has an anti-aging action, a moisturizing action and a whitening action in addition to the collagen production promoting action. Moreover, it is possible to mix | blend this composition with various cosmetics, a hair restorer, or a quasi drug according to the use.

  The present invention also provides a cosmetic comprising the above composition. The cosmetic product of the present invention contains the composition of the present invention having collagen production promoting action, anti-aging action, moisturizing action and whitening action as active ingredients, thereby providing effects such as skin beautification, moisturizing, whitening, anti-aging and hair protection. It is possible to demonstrate.

  The present invention also provides a hair restorer comprising the above composition. The hair growth agent of the present invention exhibits effects such as hair growth, hair growth and hair growth promotion by including the composition of the present invention having collagen production promoting action, anti-aging action, moisturizing action and whitening action as an active ingredient. Is possible.

  According to the method of the present invention, a novel composition having a collagen production promoting action can be obtained from an alcoholic beverage fermented with brewer's yeast. This composition has an anti-aging action, a moisturizing action and a whitening action in addition to the collagen production promoting action. Cosmetics containing this composition have effects such as smoothing the skin and protecting the hair.

  Hereinafter, embodiments of the present invention will be described in detail.

  First, the alcoholic beverage fermented with brewer's yeast used in the present invention will be described. The alcoholic beverages used in the present invention include alcoholic beverages fermented with brewer's yeast such as beer, sparkling liquor and beer-like alcoholic beverages. These alcoholic beverages may be commercially available, but may be those obtained by removing beer yeast from those after fermentation in the production process. Moreover, although these alcoholic beverages may be used independently, two or more may be mixed and used.

  Beer and happoshu are alcoholic beverages produced by fermenting with brewer's yeast using malt as the main raw material and starches such as rice, corn and starch, hops and water as auxiliary materials. In Japan's liquor tax law, beer has a malt use ratio of 66.7% by mass or more of the above raw materials excluding water, while happoshu has a malt use ratio of less than 66.7% by mass. It is prescribed.

  Beer-like alcoholic beverages do not use wheat and malt, and are made by fermenting with brewer's yeast using syrup, nitrogen source, hops and water containing at least a carbon source as raw materials. And alcoholic beverages that approximate characteristics such as foam and are classified as other miscellaneous sake (2) under the liquor tax law of Japan. Such an alcoholic beverage can be produced using, for example, pea protein as a raw material.

  Next, the manufacturing method of the composition which has the collagen production promotion effect | action of this invention is demonstrated. The method for producing a composition having a collagen production promoting action from an alcoholic beverage fermented with brewer's yeast according to the present invention includes a first step of removing carbon dioxide, a second step of removing alcohol, a polysaccharide and a protein. And a fourth step of purifying with an ion exchange resin. Hereinafter, these steps will be described in detail.

  If carbon dioxide gas is present, it is preferably removed first in order to reduce the purification efficiency of the subsequent purification step using the ion exchange resin. The step of removing carbon dioxide gas is not particularly limited as long as it is a commonly used method, but it is particularly preferable because it is simple to carry out by stirring the alcoholic beverage used in the present invention and does not involve chemical change.

  Alcohol is preferably removed to irritate the skin. The step of removing the alcohol is not particularly limited as long as it is a commonly used method, but it is particularly preferable to carry out by concentration under reduced pressure because no chemical change is involved.

  When polysaccharides and proteins contained in alcoholic beverages used in the present invention, especially polysaccharides and proteins having a molecular weight of 5000 or more are contained in the composition of the present invention, they will precipitate as insoluble components or become cloudy over time. Sometimes. Moreover, since such polysaccharides and proteins may cause allergies, it is preferable to remove them. The step of removing polysaccharides and proteins is not particularly limited as long as it is a commonly used method. For example, it can be removed by ultrafiltration, but it cannot be solubilized by concentration under reduced pressure and is removed as starch. It is also possible to do. Alcohol can be removed simultaneously by concentration under reduced pressure, which is particularly preferable.

  Color components and fragrance components such as aldehydes and ketones contained in the alcoholic beverage used in the present invention may affect the quality of cosmetics and the appearance of hair restorer, and may irritate the skin, It is preferable to remove. The step of removing these is not particularly limited as long as it is a commonly used method, but it is preferable to purify by an ion exchange resin or an adsorption resin into which an ion exchange group is introduced. In this case, in order to increase the efficiency of the ionic resin treatment, it is preferable to carry out after removing carbon dioxide gas, polymer polysaccharide and protein. Moreover, it is preferable to adjust the pH of the sample before the treatment with the ion exchange resin so that amino acids such as proline are not adsorbed and removed by the ion exchange resin. It is preferable that the ion exchange resin used is both an anion exchange resin and a cation exchange resin. As a kind, in the case of a cation exchange resin, for example, a sulfonic acid group or a carboxylic acid group is used as an exchange group. In the case of an anion exchange resin, there is no particular limitation as long as it is normally used, such as those having a quaternary ammonium group or a primary 1, 2, 3 amine group as an exchange group. Although the order in which the anion exchange resin and the cation exchange resin are treated is not particularly limited, it is preferable to adjust the pH of the sample to be added according to the treatment order. For example, when treating with an anion exchange resin first, the pH of the sample is set to 3.0 to 5.0 in advance, and the color component and scent component to be removed are ionized and treated with the anion exchange resin. After removing the anion, the cation is removed by treatment with a cation exchange resin. Conversely, when the cation exchange resin is used first, the pH of the sample is set to about 8.0 in advance.

  The method for producing a composition having an effect of promoting collagen production according to the present invention produces a composition that can be blended in cosmetics, hair restorers, and the like, and therefore further includes steps such as adjusting pH and concentration as necessary. Can be included.

  Cosmetics of the present invention are a lotion, a milky lotion, a cosmetic liquid, a general cream, a cleansing cream, a facial cleanser, a pack, a shaving cream, a tanning cream, a sunscreen cream, a sunscreen lotion, a tanning lotion, a cosmetic soap, a foundation, Includes various cosmetics such as funny, powder, lipstick, lip balm, eyeliner, eye cream, eye shadow, mascara, bath cosmetics, shampoo, rinse, hair dye, and hair cosmetics. Although the compounding quantity of the composition of this invention in cosmetics should just exist in the range of 0.001-100%, Preferably it is 0.1-40%, More preferably, it is 0.5-10%.

  The cosmetics of the present invention are manufactured by arbitrarily selecting and using in combination the various components and additives usually used in cosmetics and quasi-drugs as listed below. can do.

  For example, collagen, collagen hydrolyzate, gelatin, gelatin hydrolyzate, elastin, elastin hydrolyzate, lactoferrin, keratin, keratin hydrolysate, casein, albumin and other proteins and protein hydrolysates.

  Natural polymers such as sodium hyaluronate, xanthan gum, carrageenan, guar gum, alginic acid and salts thereof, pectin, chondroitin sulfate and salts thereof, water-soluble chitin, chitosan derivatives and salts thereof, deoxyribonucleic acid, gum arabic and tragacanth gum and derivatives thereof.

  Plant compositions such as licorice extract, aloe extract, chamomile extract, perilla extract. Microorganisms such as yeast extract. Seaweed powder and seaweed extract. Animal-derived materials such as placenta extract.

  Among carboxyvinyl polymers and salts thereof, polyacrylic acid and salts thereof, acidic polymers such as carboxymethyl cellulose and salts thereof, polyvinyl alcohol, hydroxyethyl cellulose, hydroxypropyl cellulose, methyl cellulose, polyethylene glycol, polyvinyl pyrrolidone, nitrocellulose polyvinyl methyl ether, etc. Polymer. Cationic polymers such as cationized cellulose, polyethyleneimine, and cationized guar gum.

  Lower alcohols such as ethanol and isopropyl alcohol. Polyhydric alcohols such as glycerin, 1,3-butylene glycol and propylene glycol.

  UV absorbers such as paraaminobenzoic acid UV absorbers, salicylic acid UV absorbers, cinnamic acid UV absorbers, anthraniere acid UV absorbers, and benzophenone UV absorbers.

  Vitamin A, vitamin B group, vitamin C, L-ascorbyl magnesium phosphate and derivatives thereof, vitamin D group, acetic acid d-α-tocophenol, vitamin E group, folic acid, β-carotene, γ-oryzanol, nicotinic acid, Vitamins such as pantothenic acids, biotins and ferulic acid.

  Anti-inflammatory agents such as glycyrrhizic acid and its salts, guaiazulene and its derivatives, and allantoin.

  Antioxidants such as stearic acid ester, nordihydrogua seletic acid, dibutylhydroxytoluene, butylhydroxyanisole, parahydroxyanisole, gallic propyl, sesamol, sesamolin, gossypol.

  Preservatives such as parabenzoates such as methyl parabenzoate, ethyl parabenzoate, propyl parabenzoate, and butyl parabenzoate, sorbic acid, dehydroacetic acid, phenoxyethanol, and benzoic acid.

  Metal ion sequestering agents such as edetic acid and edetic acid and its salts, phytic acid and hydroxyethanedisulfonic acid.

  Amino acids such as L-aspartic acid, DL-alanine, L-arginine, L-cysteine, L-glutamic acid, glycine, and salts thereof.

  Sugars such as maltitol, sorbitol, xylobiose, N-acetyl-D-glucosamine.

  Organic acids such as citric acid, lactic acid, α-hydroxyacetic acid, pyrrolidone carboxylic acid, and salts thereof.

  Hydrocarbons such as liquid paraffin, squalane and petrolatum.

  Fats and oils such as olive oil, palm oil, evening primrose oil, jojoba oil, castor oil, hydrogenated castor oil.

  Fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, and behenic acid.

  Higher alcohols such as myristyl alcohol, cetanol, cetostearyl alcohol, stearyl alcohol, and behenyl alcohol.

  Esters such as isopropyl myristate, isopropyl palmitate, cetyl octanoate, glyceryl trioctanoate, octyldodecyl myristate, octyl stearate, stearyl stearate.

  Phospholipids such as lecithin and its derivatives.

  Animal and plant-derived lipids such as bovine bone marrow fat and bovine brain lipid. Alkyl sulfates such as ammonium lauryl sulfate, ethanolamine lauryl sulfate, sodium lauryl sulfate, and triethanolamine lauryl sulfate.

  Polyoxyethylene (2EO) lauryl ether sulfate triethanolamine (where EO is ethylene oxide, the number before EO indicates the number of moles of ethylene oxide added), polyoxyethylene (3EO) alkyl (carbon number 11-15) Or a mixture of two or more thereof) polyoxyethylene alkyl sulfates such as sodium ether sulfate. Polyoxyethylene alkyl ether sulfates such as polyoxyethylene (3EO) tridecyl ether sodium acetate.

  Alkylbenzenesulfonates such as sodium laurylbenzenesulfonate and triethanolamine laurylbenzenesulfonate.

  Coconut oil fatty acid sarcosine sodium, lauroyl sarcosine triethanolamine, sodium lauroylmethyl-L-glutamate, coconut oil fatty acid-sodium L-glutamate, coconut oil fatty acid-L-glutamate triethanolamine. N-acyl amino acid salts such as coconut oil fatty acid sodium methyltaurine and sodium lauroylmethyltaurine, ether sulfate sodium alkane sulfonate, hydrogenated coconut oil fatty acid sodium glycerine sulfate, hydrogenated coconut oil fatty acid sodium glycerin sulfate, undecinoylamidoethylsulfosuccinate Sodium, sodium octylphenoxy dientoxyethyl sulfonate, disodium aminosulfosuccinate, dioctyl sodium sulfosuccinate, dioctyl sodium sulfosuccinate, disodium lauryl sulfosuccinate, polyoxyethylene alkyl (carbon chain 12-15) ether phosphate ( 8-10EO), sodium polyoxyethylene oleyl ether phosphate, sodium polyoxyethylene cetyl ether phosphate , Polyoxyethylene sulfo lauryl disodium succinate, sodium polyoxyethylene lauryl ether phosphate, sodium lauryl sulfoacetate, anionic surfactants such as tetradecene sodium sulfonate. Stearyldimethylammonium chloride, di (polyoxyethylene) oleylmethylammonium chloride, stearyldimethylbenzylammonium chloride, stearyltrimethylammonium chloride, tri (polyoxyethylene) stearylammonium chloride, polyoxypropylenemethyldiethylammonium chloride, myristyldimethylbenzylammonium chloride Cationic surfactants such as lauryltrimethylammonium chloride. 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, sodium undecylhydroxyethylimidazolinium betaine, undecyl-N-hydroxyethyl-N-carboxymethylimidazolinium betaine, stearyl dihydroxyethyl betaine, palm Oil fatty acid amidopropyl betaine, coconut oil alkyl-N-carboxyethyl-N-hydroxyethyl imidazolinium betaine sodium, coconut oil alkyl-N-carboxymethoxyethyl-N-carboxymethylimidazolinium disodium lauryl sulfate, N-coconut Amphoteric surfactants such as oil fatty acid acyl-L-arginine ethyl-DL-pyrrolidone carboxylate. Polyoxyethylene alkyl (carbon number 12-14) ether (7EO), polyoxyethylene octyl phenyl ether, polyoxyethylene oleyl ether, polyoxyethylene oleic acid glycerin, polyoxyethylene stearyl ether, polyoxyethylene cetyl ether, polyoxy Ethylene cetyl stearyl diether, polyoxyethylene sorbitol lanolin (40EO), polyoxyethylene nonylphenyl ether, polyoxyethylene polyoxypropylene cetyl ether, polyoxyethylene polyoxypropylene decyl tetradecyl ether, polyoxyethylene lanolin, polyoxy Nonionic surfactants such as ethylene lanolin alcohol and polyoxypropylene stearyl ether.

  Isostearic acid diethanolamide, undecylenic acid monoethanolamide, oleic acid diethanolamide, beef tallow fatty acid monoethanolamide, hardened tallow fatty acid diethanolamide, stearic acid diethanolamide, stearic acid diethylaminoethylamide, stearic acid monoethanolamide, myristic acid diethanolamide, Thickeners such as coconut oil fatty acid diethanolamide, coconut oil fatty acid ethanolamide, lauric acid isopropanolamide, lauric acid ethanolamide, lauric acid diethanolamide, lanolin fatty acid diethanolamide.

  Silicon oils such as linear or cyclic methylpolysiloxane, methylphenylpolysiloxane, dimethylpolysiloxane polyethylene glycol copolymer, dimethylpolysiloxane polypropylene copolymer, amino-modified silicone oil, and quaternary ammonium-modified silicone oil.

  Others, pH adjusters, colorants, fragrances, stabilizers, refreshing agents, blood flow promoters, keratolytic agents, astringents, wound treatment agents, foam boosters, oral preparations, deodorants / deodorants, antiallergies It can also be used together with an agent and a cell activator.

  The hair restorer of the present invention includes those that prevent hair loss, protect hair, promote hair growth, or promote hair growth of new hair after the hair is removed. The form of the hair restorer of the present invention may be lotion, aerosol, gel, jelly, cream, etc., but is not particularly limited to these forms. Although the compounding quantity of the composition of this invention in a hair restorer should just be 0.001 to 100%, Preferably it is 0.1 to 40%, More preferably, it is 0.5 to 10%. You may mix | blend a solubilizing agent, surfactant, an emulsion stabilizer, an absorption promoter, etc. with the hair restorer of this invention as needed.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated more concretely, this invention is not limited to these Examples.

(Example 1 Preparation of beer extract)
20 L of commercial beer was used as a raw material. First, the beer was stirred to generate and remove carbon dioxide. Next, alcohol was removed and beer was concentrated using a vacuum concentrator (Shibata Kagaku, R-250, etc.). After removing the polysaccharides and proteins of the high molecular weight that could not be solubilized by concentration, 1.8 L of concentrated liquid was obtained. After diluting the concentrate to 5 L and adjusting the pH to 5.0, it was treated with an anion exchange resin (Mitsubishi Chemical Diaion (registered trademark) WA21J) at a space velocity of 10 L / hr, and then the cation This was treated with an exchange resin (Mitsubishi Chemical Diaion (registered trademark) WK40). The concentration of the resulting treatment solution was adjusted so that the solid content was 5%, and at the same time, the pH was adjusted to 6.5. A predetermined amount of a preservative such as phenoxyethanol was added, and sterile filtration was performed with a 0.2 μm filter membrane to finally obtain 7 L of a colorless and transparent liquid. Hereinafter, this liquid is referred to as a beer extract.

  The component analysis of the said beer extract was performed. Specifically, the pH value is determined by the glass electrode method, the solid content is determined by the drying loss method at 105 ° C., the total nitrogen amount is determined by the Kjeldahl method, the total sugar amount is determined by the phenol-sulfuric acid method based on glucose, and the linear oligosaccharide amount is determined by The amount of free amino acids was measured with an amino analyzer JCL500 (JEOL Ltd.) by sugar analysis HPLC (Nippon Dionex). The results are shown in Table 1.

(Example 2 Collagen production promotion test)
To a plastic petri dish for cell culture with a diameter of 35 mm, 2 mL of DMEM medium containing 10% FBS and 4 × 10 ⁵ human-derived normal fibroblasts were added at a petri dish and cultured at 37 ° C. for 24 hours at 5% CO ₂ concentration. . After confirming that the cells were confluent, the cells were thoroughly washed with a phosphate buffer, and a serum-free DMEM medium containing a beer extract at a predetermined concentration (v / v) was added and cultured for 72 hours. The amount of collagen produced by the cells was measured by measuring the amount of collagen in the culture supernatant using PIP EIA Kit manufactured by Takara Biomedical. The thing which does not add beer extract was set as control. Table 2 shows the measurement results.

  As shown in Table 2, it was found that the beer extract has a remarkable collagen production promoting action, and the action is concentration-dependent.

(Example 3 Cell activation test)
To a plastic petri dish for cell culture with a diameter of 35 mm, 2 mL of DMEM medium containing 10% FBS and 1.5 × 10 ⁵ human-derived normal fibroblasts were added at a petri dish at 5% CO ₂ concentration at 37 ° C. for 24 hours. Cultured. After confirming cell extension and colonization, the medium is discarded, the cells are washed three times with a phosphate buffer, and a DMEM medium containing 10% FBS containing a beer extract at a predetermined concentration (v / v) is further added. Cultured for 48 hours. Cells were detached by trypsin-EDTA treatment, and the number of cells was counted with a hemocytometer. The results are shown in Table 3.

  As shown in Table 3, the beer extract was found to have a remarkable cell growth promoting action, that is, a cell activation action.

(Example 4 moisturizing effect test)
To a plastic petri dish for cell culture having a diameter of 35 mm, 2 mL of DMEM medium containing 10% FBS and 1.5 × 10 ⁵ human-derived normal fibroblasts were added at a rate of 1.5 × 10 ⁵ / Petri dish, 5% CO ₂ concentration at 37 ° C. for 7 days. Cultured. After confirming that the cells were confluent, the cells were washed 2 × 3 times with a phosphate buffer or a phosphate buffer containing 1% of beer extract. In order to give a dry load to the cells, the petri dish lid was removed, and the cells were dried for 5 hours at a humidity of 95% or higher at 37 ° C. with a 5% CO ₂ concentration. After the drying treatment, the number of viable cells was measured by MTT assay. The ratio of viable cells was calculated according to the formula (viable cell ratio = viable cells of beer extract-containing phosphate buffer treatment / viable cells of phosphate buffer treatment). The results are shown in Table 4.

  As shown in Table 4, the beer extract was found to have a clear moisturizing action.

(Example 5 Melanin production inhibition test)
10 mL of DMEM medium containing 10% FBS in a cell culture bottle having a surface area of 25 cm ² and 1 mL of a suspension of B16 cells that are melanin producing cells (2.5 × 10 ⁴ cells / mL, DMEM medium containing 10% FBS) The mixture was added and cultured at 37 ° C. for 24 hours at a CO ₂ concentration of 5%. A DMEM medium containing 10% FBS containing a beer extract at a predetermined concentration (v / v) was added and cultured for 3 days. Then, the same amount of medium and sample were replaced, and further cultured for 3 days. Cells were detached by trypsin-EDTA treatment, and the obtained cells were centrifuged at 1000 ppm for 1 minute to remove the supernatant, and the hue of the cells was observed. A sample containing 0.01% (v / v) kojic acid was used as a positive control, and a sample containing no sample was used as a control. The results are shown in Table 5.

  As shown in Table 5, the beer extract was found to have a remarkable melanin production inhibitory action, that is, a whitening action.

(Example 6: Production of lotion)
According to the formulation of Table 6, the raw materials (1) to (10) were mixed and stirred at 80 ° C., dissolved, and cooled to room temperature to obtain a lotion. All of the obtained lotions were clear and stable without causing cloudiness even when stored for 3 months under conditions of 40 ° C. and RH 75%.

  This lotion was subjected to a sensory test by 10 professional panelists. The evaluation was carried out in five grades for the following items (1) to (3). Table 7 shows the average score of 10 panelists.

(1) Moist skin 2. Slightly soft 2. Normal 4. Slightly moist 5. Moist (2) Smooth skin 1 Rough 2 Roughly 3 Normal 4 Slightly smooth 5 Smooth (3) Skin stickiness 1 Sticky 2 Slightly sticky 3 Normal 4 Slightly refreshing 5 Refreshing

  As shown in Table 7, it turned out that the implementation goods which mix | blended the beer extract have the moisture retention and skin smoothness which were excellent compared with the comparison goods without a beer extract combination.

(Example 7 Production of shampoo agent)
According to the formulation in Table 8, the raw materials (1) to (8) were mixed and stirred at 70 ° C. and cooled to room temperature to obtain a shampoo agent.

  When the obtained product was used to wash the hair, it was found that the hair feel was smooth and moisturized.

(Example 8 Production of cream)
In accordance with the formulation shown in Table 9, the materials (1) to (7) were mixed and stirred at 80 ° C., and the materials (8) to (12) were mixed and stirred at 80 ° C., and homogenized. The mixture was cooled to room temperature while stirring to obtain a cream.

  The obtained product was found not to be sticky during use and to moisturize the skin.

(Example 9 Production of body gel)
According to the prescription in Table 10, the raw materials (1) to (8) are mixed and stirred and dissolved.

  The obtained product was found not to be sticky during use and to moisturize the skin.

(Example 10 Production of hair pack agent)
According to the formulation shown in Table 11, the materials (1) and (2) were mixed and stirred at 80 ° C., and the materials (3) to (13) were mixed and stirred at 80 ° C., and mixed at 80 ° C. While stirring, the raw materials (14) and (15) were further added, mixed and stirred, and cooled to room temperature to obtain a hair pack agent.

  When the hair was treated with the obtained product, it was found that the hair feels smooth and moisturizes the hair.

Claims (6)

A method for producing a composition having a collagen production promoting action from an alcoholic beverage fermented with brewer's yeast,
A first step of removing carbon dioxide;
A second step of removing alcohol by vacuum concentration and insolubilizing polysaccharides and proteins ;
A third step of removing polysaccharides and proteins;
And a fourth step of purification using an ion exchange resin.   The production method according to claim 1, wherein the alcoholic beverage fermented with brewer's yeast is at least one alcoholic beverage selected from the group consisting of beer, sparkling liquor and beer-like alcoholic beverages.   The method according to claim 1 or 2, wherein the first step is performed by stirring an alcoholic beverage. The fourth step, the manufacturing method according to any one of claims 1 to 3, characterized in that the purification by anion exchange resin and cation exchange resin. The composition obtained by the method of any one of Claims 1-4 . A cosmetic comprising the composition according to claim 5 .