TR200202196T2 - Homolog yeniden kombine etmeyi iyileştirme yöntemleri - Google Patents
Homolog yeniden kombine etmeyi iyileştirme yöntemleriInfo
- Publication number
- TR200202196T2 TR200202196T2 TR2002/02196T TR200202196T TR200202196T2 TR 200202196 T2 TR200202196 T2 TR 200202196T2 TR 2002/02196 T TR2002/02196 T TR 2002/02196T TR 200202196 T TR200202196 T TR 200202196T TR 200202196 T2 TR200202196 T2 TR 200202196T2
- Authority
- TR
- Turkey
- Prior art keywords
- agent
- homologous recombination
- methods
- alteration
- antibody
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4712—Cystic fibrosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Pulmonology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Led Devices (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Electrical Discharge Machining, Electrochemical Machining, And Combined Machining (AREA)
Abstract
Bu bulus, hedef bir DNA'nin seçilen bir bölgesinde, örn. bir hücrenin kromozomal DNA'sinda bir baskalasimi yükseltmek için bir yöntemle ilgilidir. Yöntem, bölgede (a) seçilmis DNA sekansi içeren çift sarmalli DNA sekansi; (b) homolog birlesmeyi destekleyen bir madde, örn. bir Rad52 proteini veya bunun fonksiyonel bir parçasi; ve (c) homolog olmayan uç eklenmesini inhibe eden madde örn. bir anti-Ku antikoru veya bir Ku-birlesme oligomer veya polimeri gibi bir madde saglamak ve baskalasimin gerçeklesmesini mümkün kilmaktan meydana gelir. Homolog olmayan uç eklenmesini inhibe eden madde, örn. bir anti-Ku antikoru gibi Ku'yu etkisizlestiren bir madde tercihen lokal olarak verilir. (a), (b) ve (c) maddeleri tercih edildigi gibi birlikte veya ayri ayri verilebilir.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/525,160 US6569681B1 (en) | 2000-03-14 | 2000-03-14 | Methods of improving homologous recombination |
Publications (1)
Publication Number | Publication Date |
---|---|
TR200202196T2 true TR200202196T2 (tr) | 2003-01-21 |
Family
ID=24092175
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TR2002/02196T TR200202196T2 (tr) | 2000-03-14 | 2001-03-13 | Homolog yeniden kombine etmeyi iyileştirme yöntemleri |
Country Status (10)
Country | Link |
---|---|
US (1) | US6569681B1 (tr) |
EP (1) | EP1263972B1 (tr) |
JP (1) | JP2003526376A (tr) |
AT (1) | ATE525472T1 (tr) |
AU (1) | AU2001245637A1 (tr) |
CA (1) | CA2403113A1 (tr) |
DK (1) | DK1263972T3 (tr) |
MX (1) | MXPA02009044A (tr) |
TR (1) | TR200202196T2 (tr) |
WO (1) | WO2001068882A2 (tr) |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL154099A0 (en) * | 2000-07-27 | 2003-07-31 | Univ Delaware | A method and kit for enhancing oligonucleotide-mediated gene alteration |
DK1395669T3 (da) * | 2001-01-26 | 2009-11-16 | Selexis Sa | Matriks bindingsregioner og fremgangsmåder til anvendelse af disse |
CA2473741C (en) * | 2002-01-18 | 2015-12-22 | Morphotek, Inc. | A method for generating engineered cells for locus specific gene regulation and analysis |
GB0214896D0 (en) * | 2002-06-27 | 2002-08-07 | Novartis Forschungsstiftung | Gene for increased somatic recombination |
FR2846337B1 (fr) | 2002-10-28 | 2007-06-22 | Centre Nat Rech Scient | Procede pour inserer un acide nucleique d'interet dans une cellule procaryote ou eucaryote par recombinaison homologue |
AU2003296438A1 (en) * | 2002-12-24 | 2004-07-29 | North Carolina State University | Increasing gamete production with a gene switch |
PL1959011T3 (pl) | 2003-10-24 | 2012-07-31 | Selexis Sa | Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do macierzy |
JP4050712B2 (ja) | 2004-02-27 | 2008-02-20 | 独立行政法人科学技術振興機構 | 相同組換えを行わせる方法 |
US9243043B2 (en) * | 2004-04-02 | 2016-01-26 | Dsm Ip Assets B.V. | Filamentous fungal mutants with improved homologous recombination efficiency |
US20060110390A1 (en) * | 2004-08-25 | 2006-05-25 | Myogen, Inc. | Inhibition of Ku as a treatment for cardiovascular diseases |
JP4625318B2 (ja) * | 2004-12-06 | 2011-02-02 | 財団法人野田産業科学研究所 | 相同組換え頻度が上昇した形質転換菌 |
EP2045327B8 (en) | 2005-03-08 | 2012-03-14 | BASF Plant Science GmbH | Expression enhancing intron sequences |
EP1871868A2 (en) | 2005-04-15 | 2008-01-02 | North Carolina State University | Methods and compositions to modulate adhesion and stress tolerance in bacteria |
JP2007222055A (ja) * | 2006-02-22 | 2007-09-06 | Noda Inst For Scient Res | 染色体領域欠失株の作製方法 |
JP2008263927A (ja) * | 2007-04-25 | 2008-11-06 | Noda Inst For Scient Res | 染色体大領域の欠失方法 |
WO2008138835A1 (en) * | 2007-05-09 | 2008-11-20 | Novozymes A/S | Expression cloning method suitable for selecting library clones producing a polypeptide of interest |
NZ582330A (en) | 2007-06-27 | 2012-05-25 | Marinepolymer Tech Inc | COMPLEXES OF IL-15 AND IL-15Ra (RECEPTOR ALPHA) AND USES THEREOF |
EP2462230B1 (en) * | 2009-08-03 | 2015-07-15 | Recombinetics, Inc. | Methods and compositions for targeted gene modification |
AU2010282340B2 (en) | 2009-08-13 | 2016-12-22 | The Johns Hopkins University | Methods of modulating immune function |
WO2011078662A1 (en) * | 2009-12-21 | 2011-06-30 | Keygene N.V. | Dsrna for improved genetic modification of plant dna |
EP2516652B1 (en) * | 2009-12-21 | 2014-11-05 | Keygene N.V. | Improved techniques for transfecting protoplasts |
GB201009732D0 (en) * | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
US20140304847A1 (en) * | 2011-06-07 | 2014-10-09 | Ralf Kühn | Recombination efficiency by inhibition of nhej dna repair |
US20150307876A1 (en) * | 2012-02-10 | 2015-10-29 | Regents Of The University Of Minnesota | Dna assimilation |
EP2911684B1 (en) | 2012-10-24 | 2019-06-19 | Novartis Ag | Il-15r alpha forms, cells expressing il-15r alpha forms, and therapeutic uses of il-15r alpha and il-15/il-15r alpha complexes |
WO2015066205A1 (en) * | 2013-10-29 | 2015-05-07 | Rutgers, The State University Of New Jersey | Fast genetically modified organism and systems for precise genome editing and targeted genetic manipulation through enhanced homologous recombination |
JP6655061B2 (ja) | 2014-07-29 | 2020-02-26 | ノバルティス アーゲー | 状態を治療するためのil−15およびil−15rアルファヘテロ二量体用量増加レジメン |
AU2015323966A1 (en) | 2014-09-29 | 2017-03-16 | Alexion Pharmaceuticals, Inc. | Methods of treating mucopolysaccharidosis IIIB (MPSIIIB) |
EP3420080B1 (en) | 2016-02-22 | 2019-08-21 | Caribou Biosciences, Inc. | Methods for modulating dna repair outcomes |
EP3570870A1 (en) | 2017-01-20 | 2019-11-27 | Novartis AG | Combination therapy for the treatment of cancer |
CA3046120A1 (en) | 2017-01-20 | 2018-07-26 | Novartis Ag | Combination therapy for the treatment of cancer |
JP7084021B2 (ja) * | 2018-01-24 | 2022-06-14 | 公立大学法人大阪 | ターゲティングベクター |
CR20220367A (es) | 2020-02-05 | 2022-08-30 | Novartis Ag | Célula cho que expresa heterodímeros il-15 |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5272071A (en) | 1989-12-22 | 1993-12-21 | Applied Research Systems Ars Holding N.V. | Method for the modification of the expression characteristics of an endogenous gene of a given cell line |
US6010908A (en) | 1992-08-21 | 2000-01-04 | The Regents Of The University Of California | Gene therapy by small fragment homologous replacement |
US5641670A (en) | 1991-11-05 | 1997-06-24 | Transkaryotic Therapies, Inc. | Protein production and protein delivery |
WO1993022443A1 (en) * | 1992-04-24 | 1993-11-11 | Sri International | In vivo homologous sequence targeting in eukaryotic cells |
US5780296A (en) | 1995-01-17 | 1998-07-14 | Thomas Jefferson University | Compositions and methods to promote homologous recombination in eukaryotic cells and organisms |
GB9700574D0 (en) | 1997-01-13 | 1997-03-05 | Cancer Res Campaign Tech | Methods and means relating to retrotransposon and retroviral integration |
ATE237136T1 (de) | 1997-01-13 | 2003-04-15 | Kudos Pharm Ltd | Assay, verbindungen, therapie und nachweisverfahren um zelluläre dns-reparatur- aktivität zu modulieren |
JP4062366B2 (ja) * | 1997-01-17 | 2008-03-19 | マキシジェン,インコーポレイテッド | 再帰的配列組換えによる全細胞および生物の進化 |
US5986055A (en) | 1997-11-13 | 1999-11-16 | Curagen Corporation | CDK2 interactions |
DE19755952C1 (de) | 1997-12-16 | 1998-12-10 | Curt Niebling | Dünnwandiges, elektro-magnetisch abschirmendes, tiefgezogenes Formteil, Verfahren zu dessen Herstellung, und ein bei diesem Verfahren einsetzbares Zwischenprodukt |
US6441158B1 (en) | 1997-12-31 | 2002-08-27 | Medical College Of Georgia Research Institute, Inc. | Oligomers that bind to ku protein |
US6180850B1 (en) | 1998-09-02 | 2001-01-30 | Pioneer Hi-Bred International, Inc. | Maize Ku70 orthologue and uses thereof |
EP1093523A1 (en) | 1999-05-05 | 2001-04-25 | Pioneer Hi-Bred International, Inc. | Maize rad50 orthologue and uses of therof |
-
2000
- 2000-03-14 US US09/525,160 patent/US6569681B1/en not_active Expired - Lifetime
-
2001
- 2001-03-13 DK DK01918578.4T patent/DK1263972T3/da active
- 2001-03-13 JP JP2001567365A patent/JP2003526376A/ja active Pending
- 2001-03-13 AU AU2001245637A patent/AU2001245637A1/en not_active Abandoned
- 2001-03-13 MX MXPA02009044A patent/MXPA02009044A/es active IP Right Grant
- 2001-03-13 AT AT01918578T patent/ATE525472T1/de not_active IP Right Cessation
- 2001-03-13 CA CA002403113A patent/CA2403113A1/en not_active Abandoned
- 2001-03-13 TR TR2002/02196T patent/TR200202196T2/tr unknown
- 2001-03-13 WO PCT/US2001/007870 patent/WO2001068882A2/en active Application Filing
- 2001-03-13 EP EP01918578A patent/EP1263972B1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DK1263972T3 (da) | 2012-01-02 |
AU2001245637A1 (en) | 2001-09-24 |
WO2001068882A2 (en) | 2001-09-20 |
EP1263972A2 (en) | 2002-12-11 |
EP1263972B1 (en) | 2011-09-21 |
US6569681B1 (en) | 2003-05-27 |
CA2403113A1 (en) | 2001-09-20 |
WO2001068882A3 (en) | 2002-03-14 |
MXPA02009044A (es) | 2003-04-25 |
ATE525472T1 (de) | 2011-10-15 |
JP2003526376A (ja) | 2003-09-09 |
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