WO2002004666A3 - Decondensation of dna - Google Patents

Decondensation of dna Download PDF

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Publication number
WO2002004666A3
WO2002004666A3 PCT/GB2001/002934 GB0102934W WO0204666A3 WO 2002004666 A3 WO2002004666 A3 WO 2002004666A3 GB 0102934 W GB0102934 W GB 0102934W WO 0204666 A3 WO0204666 A3 WO 0204666A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
sperm
decondensation
thiol
dna
Prior art date
Application number
PCT/GB2001/002934
Other languages
French (fr)
Other versions
WO2002004666A2 (en
Inventor
Willem Rens
Malcolm Andrew Ferguson-Smith
Patricia Caroline Mary Brien
Fengtang Yang
Original Assignee
Univ Cambridge Tech
Willem Rens
Malcolm Andrew Ferguson-Smith
Patricia Caroline Mary Brien
Fengtang Yang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Cambridge Tech, Willem Rens, Malcolm Andrew Ferguson-Smith, Patricia Caroline Mary Brien, Fengtang Yang filed Critical Univ Cambridge Tech
Priority to AU2001266225A priority Critical patent/AU2001266225A1/en
Publication of WO2002004666A2 publication Critical patent/WO2002004666A2/en
Publication of WO2002004666A3 publication Critical patent/WO2002004666A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Decondensation of DNA is accomplished by treating DNA-containing cells with a thiol and, simultaneously or subsequently, with anionic surfactant wherein the cells are exposed to the thiol for less than 20 minutes. The cells are preferably sperm cells and the method is particularly useful in livestock sperm such as bovine sperm. Sperm cells may be subjected to fluorescent in situ hybridisation after decondensation. The invention also provides kits comprising thiol and anionic surfactant and treatment instructions. The methods and kits are useful in assessing the efficiency of sperm sorting procedures.
PCT/GB2001/002934 2000-07-10 2001-07-03 Decondensation of dna WO2002004666A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001266225A AU2001266225A1 (en) 2000-07-10 2001-07-03 Decondensation of dna

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0016920.1A GB0016920D0 (en) 2000-07-10 2000-07-10 Decondensation of DNA
GB0016920.1 2000-07-10

Publications (2)

Publication Number Publication Date
WO2002004666A2 WO2002004666A2 (en) 2002-01-17
WO2002004666A3 true WO2002004666A3 (en) 2003-03-13

Family

ID=9895373

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/002934 WO2002004666A2 (en) 2000-07-10 2001-07-03 Decondensation of dna

Country Status (3)

Country Link
AU (1) AU2001266225A1 (en)
GB (1) GB0016920D0 (en)
WO (1) WO2002004666A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4323571B2 (en) 1997-01-31 2009-09-02 エックスワイ， インコーポレイテッド Optical device
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
CA2822983C (en) 2000-11-29 2017-05-09 Xy, Llc System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
BRPI0313163B1 (en) 2002-08-01 2015-11-17 Univ Colorado State low pressure sperm cell separation system
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
AU2003265471B2 (en) 2002-08-15 2009-08-06 Xy, Llc. High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
DK2305173T3 (en) 2003-03-28 2016-08-22 Inguran Llc Apparatus and method for providing sorted particles
US20060263829A1 (en) 2003-05-15 2006-11-23 Evans Kenneth M Efficient haploid cell sorting flow cytometer systems
AU2005229073B2 (en) 2004-03-29 2010-08-19 Inguran, Llc Sperm suspensions for use in insemination
US7833147B2 (en) 2004-07-22 2010-11-16 Inguran, LLC. Process for enriching a population of sperm cells
US8110185B2 (en) 2008-10-03 2012-02-07 St. Michael's Hospital Method for preventing and treating cardiovascular diseases with BRCA1
GR1008033B (en) * 2012-03-29 2013-11-18 Βασιλειος Τσιλιβακος Method for the exploration of the presence of endocellular infectious agents in sperm cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993022463A2 (en) * 1992-05-07 1993-11-11 Ig Laboratories, Inc. Methods for high resolution gene mapping

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993022463A2 (en) * 1992-05-07 1993-11-11 Ig Laboratories, Inc. Methods for high resolution gene mapping

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
AMERICAN JOURNAL OF VETERINARY RESEARCH. UNITED STATES AUG 1980, vol. 41, no. 8, August 1980 (1980-08-01), pages 1190 - 1196, ISSN: 0002-9645 *
DATABASE MEDLINE [online] April 2001 (2001-04-01), RENS W ET AL: "An X-Y paint set and sperm FISH protocol that can be used for validation of cattle sperm separation procedures.", XP002225062, Database accession no. NLM11277872 *
DATABASE MEDLINE [online] August 1980 (1980-08-01), JOHNSON L ET AL: "Maturation of equine epididymal spermatozoa.", XP002225061, Database accession no. NLM7447113 *
DATABASE MEDLINE [online] October 1992 (1992-10-01), HAN T L ET AL: "Detection of chromosome 17- and X-bearing human spermatozoa using fluorescence in situ hybridization.", XP002225060, Database accession no. NLM1418988 *
KAWARASAKI T ET AL: "Verification of flow cytometrically-sorted X- and Y-bearing porcine spermatozoa and reanalysis of spermatozoa for DNA content using the fluorescence in situ hybridization (FISH) technique.", THERIOGENOLOGY, vol. 50, no. 4, 1998, pages 625 - 635, XP002225058, ISSN: 0093-691X *
KOBAYASHI J ET AL: "Fluorescence in situ hybridization with Y chromosome-specific probe in decondensed bovine spermatozoa.", THERIOGENOLOGY, vol. 52, no. 6, 15 October 1999 (1999-10-15), pages 1043 - 1054, XP002225057, ISSN: 0093-691X *
MOLECULAR REPRODUCTION AND DEVELOPMENT. UNITED STATES OCT 1992, vol. 33, no. 2, October 1992 (1992-10-01), pages 189 - 194, ISSN: 1040-452X *
MOLINA JORGE ET AL: "Influence of incubation on the chromatin condensation and nuclear stability of human spermatozoa by flow cytometry.", HUMAN REPRODUCTION (OXFORD), vol. 10, no. 5, 1995, pages 1280 - 1286, XP009003038, ISSN: 0268-1161 *
REPRODUCTION (CAMBRIDGE, ENGLAND) ENGLAND APR 2001, vol. 121, no. 4, April 2001 (2001-04-01), pages 541 - 546, ISSN: 1470-1626 *
RODRIGUEZ H ET AL: "Nuclear chromatin decondensation of spermatozoa in vitro: a method for evaluating the fertilizing ability of ovine semen.", INTERNATIONAL JOURNAL OF ANDROLOGY. DENMARK APR 1985, vol. 8, no. 2, April 1985 (1985-04-01), pages 147 - 158, XP009003036, ISSN: 0105-6263 *

Also Published As

Publication number Publication date
AU2001266225A1 (en) 2002-01-21
WO2002004666A2 (en) 2002-01-17
GB0016920D0 (en) 2000-08-30

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